view shear_sv.xml @ 4:a82400332451

Use shear_wrapper.py to generate reference indexes when needed

<?xml version="1.0"?>
<tool id="shear_sv" name="SHEAR-SV" version="0.0.1">
  <description>find SVs and estimate the heterogeneity levels from a BAM</description>
  <requirements>
    <requirement type="package" version="0.1.2">shear</requirement>
    <requirement type="package" version="0.1.18">samtools</requirement>
    <requirement type="package" version="0.6.2">bwa</requirement>
    <requirement type="package" version="1.0.1">crest</requirement>
    <requirement type="package" version="latest">kent</requirement>
  </requirements>
  <!--
  <version_command></version_command>
  -->
  <command interpreter="python">
    shear_wrapper.py -j \$SHEAR_JAR_PATH/SHEAR.jar --command sv 
    -p $prefix 
    -b $bamfile 
     #if $genomeSource.refGenomeSource == 'indexed':
       -f $genomeSource.ref_fastas.fields.path 
       -i $genomeSource.bwa_indices.fields.path 
       -t $genomeSource.twobit_indices.fields.path
     #elif $genomeSource.refGenomeSource == 'svidx':
       -f $genomeSource.svidx.extra_files_path/${genomeSource.svidx.metadata.base_name}.fa
       -i $genomeSource.svidx.extra_files_path/${genomeSource.svidx.metadata.base_name}.fa
       -t $genomeSource.svidx.extra_files_path/${genomeSource.svidx.metadata.base_name}.2bit
     #else:
       -f $genomeSource.ref_fasta
       #if $genomeSource.save_svidx:
         -D $sv_idx.extra_files_path
       #end if
     #end if
     --report $report
     --sdi $sdi
  </command>
  <inputs>
    <param name="bamfile" type="data" format="bam" label="BAM alignment file containing the input sequences to the assembly."/> 
    <param name="prefix" type="text" value="shear_sv" label="Prefix for all generated files">
      <validator type="regex" message="Prefix should start with a letter and contain only letter, digit, and '_' or '-' characters">[a-zA-Z0-9][_a-zA-Z0-9-]*</validator>
    </param>
    <!-- reference data -->
    <conditional name="genomeSource">
      <param name="refGenomeSource" type="select" label="Will you select a reference genome from your history or use a cached file?">
        <option value="indexed" selected="true">Use a cached reference genome</option>
        <option value="history">Use fasta from the history</option>
        <option value="svidx">Use SHEAR sv index from the history</option>
      </param>
      <when value="indexed">
        <param name="ref_fastas" type="select" label="Select a reference genome fasta">
          <options from_data_table="all_fasta">
            <filter type="sort_by" column="2" />
            <validator type="no_options" message="No reference fasta files are available" />
          </options>
        </param>
        <param name="twobit_indices" type="select" label="Select the reference genome 2bit">
          <options from_data_table="twobit">
            <filter type="sort_by" column="0" />
            <!--
            <filter type="data_meta" ref="ref_fastas" key="dbkey" column="0" />
            -->
            <validator type="no_options" message="No indexes are available" />
          </options>
        </param>
        <param name="bwa_indices" type="select" label="Select the reference genome bwa indices">
          <options from_data_table="bwa_indexes">
            <filter type="sort_by" column="2" />
            <!--
            <filter type="data_meta" ref="ref_fastas" key="dbkey" column="0" />
            -->
            <validator type="no_options" message="No indexes are available" />
          </options>
        </param>
      </when>
      <when value="history">
        <param name="ref_fasta" type="data" format="fasta" label="Select a reference from history" />
        <param name="save_svidx" type="boolean" truevalue="yes" falsevalue="no" label="Save sv generated reference indexes"/> 
      </when>
      <when value="svidx">
        <param name="svidx" type="data" format="shear.svidx" label="Select a reference from history" />
      </when>
    </conditional>
  </inputs>
  <stdio>
    <exit_code range="1:" level="fatal" description="Error" />
  </stdio>
  <outputs>
    <data format="shear.sdi" name="sdi" label="${tool.name} on ${on_string}: ${prefix}.sdi" />
    <data format="txt" name="report" label="${tool.name} on ${on_string}: ${prefix}.report" />
    <data format="shear.svidx" name="sv_idx" label="${tool.name} on ${on_string}: ${prefix}.svidx">
      <filter>genomeSource['refGenomeSource'] == 'history' and genomeSource['save_svidx'] == True</filter>
    </data>
  </outputs>
  <tests>
    <test>
      <param name="prefix" value="shear_test" />
      <param name="bamfile" value="simulated-data.bam" ftype="bam" />
      <param name="refGenomeSource" value="history"/>
      <param name="ref_fasta" value="syn.fa" ftype="fasta" />
      <output name="sdi"  file="shear_sv.sdi"/>
      <output name="report"  file="shear_sv.report"/>
    </test>
  </tests>
  <help>
SHEAR-SV will process a BAM alignment file to find SVs and estimate the
heterogeneity level of those SVs. It will output a *.report file reporing the
SVs and their heterogeneity levels, and it will output a *.sdi file that can be
used for SHEAR-Assemble to reconstruct the new genome.
  </help>
</tool>