Mercurial > repos > jjohnson > peptideshaker
comparison README.rst @ 1:fa76abf69433 draft
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit e09348a0c372d4355c5dda6519e52ff17e0e8621-dirty
| author | jjohnson |
|---|---|
| date | Fri, 08 Jun 2018 15:58:53 -0400 |
| parents | 8b99cb00e1c4 |
| children | eea7e945f479 |
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| 0:8b99cb00e1c4 | 1:fa76abf69433 |
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| 1 GalaxyP - PeptideShaker | 1 GalaxyP - PeptideShaker |
| 2 ======================= | 2 ======================= |
| 3 | 3 |
| 4 - Home: <https://github.com/galaxyproteomics/tools-galaxyp/> | 4 - Home: <https://github.com/galaxyproteomics/tools-galaxyp/> |
| 5 - Galaxy Tool Shed: <http://toolshed.g2.bx.psu.edu/view/galaxyp/peptideshaker> | 5 - Galaxy Tool Shed: <http://toolshed.g2.bx.psu.edu/view/galaxyp/peptideshaker> |
| 6 - Tool ID: `peptideshaker`, `search_gui` | 6 - Tools ID: `peptide_shaker`, `search_gui` |
| 7 | 7 |
| 8 | 8 |
| 9 Description | 9 Description |
| 10 ----------- | 10 ----------- |
| 11 | 11 |
| 12 Perform protein identification combining numerous search engines (using SearchGUI) followed by peptide and protein inference with PeptideShaker. | 12 Perform protein identification combining numerous search engines (using SearchGUI) followed by peptide and protein inference with PeptideShaker. |
| 13 | 13 |
| 14 Includes tool wrappers for SearchGUI and PeptideShaker. The SearchGUI tool takes any number of mgf files and performs searches on these. It creates a large zip archive with all search results, the original database and spectra. This can then be fed to the PeptideShaker tool which merges the results and performs peptide and protein inference. | 14 Includes tool wrappers for FastaCLI, IdentificationParametersCLI, SearchGUI and PeptideShaker. |
| 15 | |
| 16 | |
| 17 | |
| 18 FastaCLI adds decoy sequences to any fasta file. | |
| 19 | |
| 20 IdentificationParametersCLI allows to create a compressed zip file with a parameters (par) file which can be (re)used later to parameterize SearchGUI or PeptideShaker, and the fasta file referred. | |
| 21 | |
| 22 The SearchGUI tool takes any number of mgf files and performs searches on these. It creates a large zip archive with all search results, the original database and spectra. | |
| 23 | |
| 24 This can then be fed to the PeptideShaker tool which merges the results and performs peptide and protein inference. | |
| 15 | 25 |
| 16 | 26 |
| 17 General Requirements | 27 General Requirements |
| 18 -------------------- | 28 -------------------- |
| 19 | 29 |
| 29 | 39 |
| 30 MSAmanda on linux | 40 MSAmanda on linux |
| 31 ----------------- | 41 ----------------- |
| 32 | 42 |
| 33 Running MS Amanda on Linux requires that you have Mono installed. Mono 3.2.1 or newer is required. If you install via the toolshed Mono should be installed automatically, however if this does not work you can install it manually. | 43 Running MS Amanda on Linux requires that you have Mono installed. Mono 3.2.1 or newer is required. If you install via the toolshed Mono should be installed automatically, however if this does not work you can install it manually. |
| 34 | 44 |
| 35 On ubuntu Mono can be installed as follows | 45 On ubuntu Mono can be installed as follows |
| 36 | 46 |
| 37 sudo apt-get install mono-runtime | 47 sudo apt-get install mono-runtime |
| 38 sudo apt-get install libmono-system-core4.0-cil | 48 sudo apt-get install libmono-system-core4.0-cil |
| 39 | 49 |
| 40 For more help on installing Mono please see http://www.mono-project.com/download. | 50 For more help on installing Mono please see http://www.mono-project.com/download. |
| 41 | 51 |
| 42 Note | 52 Note |
| 43 ---- | 53 ---- |
| 44 | 54 |
| 45 - Requires Galaxy release v15.10 or later, which fixes the installation of package_peptideshaker_1_1 and provides a searchgui_archive datatype that includes a version | 55 - Requires Galaxy release v16.01 or later. |
| 46 | |
| 47 - PeptideShaker may require xvfb to simulate an X environment if this is installed on a headless server. | |
| 48 | 56 |
| 49 See: | 57 See: |
| 50 | 58 |
| 51 * <https://code.google.com/p/peptide-shaker/> | 59 * <http://compomics.github.io/projects/peptide-shaker.html/> |
| 52 * <https://code.google.com/p/searchgui/> | 60 * <http://compomics.github.io/projects/searchgui.html/> |
| 53 | 61 |
| 54 | 62 |
| 55 Search GUI - Fixed and Variable Modifications | 63 Search GUI - Fixed and Variable Modifications |
| 56 ------------------------------------------- | 64 ------------------------------------------- |
| 57 | 65 |
| 58 - Options for modifications are read from local data file: searchgui_mods.loc | 66 - Options for modifications are read from local data file: searchgui_mods.loc |
| 59 This is copied from searchgui_mods.loc.sample on the first installation. | 67 This is copied from searchgui_mods.loc.sample on the first installation. |
| 60 | 68 |
| 61 The modifications are from: https://github.com/compomics/compomics-utilities/blob/master/src/main/java/com/compomics/util/experiment/identification/search_parameters_cli/IdentificationParametersInputBean.java | 69 The modifications are from: https://github.com/compomics/compomics-utilities/blob/master/src/main/java/com/compomics/util/experiment/identification/search_parameters_cli/IdentificationParametersInputBean.java |
| 62 | 70 |
| 63 GalaxyP Community | 71 GalaxyP Community |
| 64 ----------------- | 72 ----------------- |