view mothur/tools/mothur/dist.seqs.xml @ 3:6b358d0f17b4 draft

Update to Mothur version 1.24
author Jim Johnson <jj@umn.edu>
date Wed, 05 Sep 2012 19:49:43 -0500
parents e076d95dbdb5
children a9d51d14f9e9
line wrap: on
line source

<tool id="mothur_dist_seqs" name="Dist.seqs" version="1.19.0">
 <description>calculate uncorrected pairwise distances between aligned sequences</description>
 <command interpreter="python">
  mothur_wrapper.py 
  --cmd='dist.seqs'
  --result='^mothur.\S+\.logfile$:'$logfile,'^\S+\.dist$:'$out_dist
  --outputdir='$logfile.extra_files_path'
  --fasta=$fasta
  #if len($calc.__str__) > 0:
   --calc=$calc
  #end if
  $countends
  #if float($cutoff.__str__) > 0.0:
   --cutoff=$cutoff
  #end if
  #if len($output.__str__) > 0:
   --output=$output
  #end if
  --processors=8
 </command>
 <inputs>
  <param name="fasta" type="data" format="align" label="fasta - Alignment Fasta"/>
  <param name="calc" type="select" label="calc - Calc Method - Gap Penality" help="">
   <option value="">use default</option>
   <option value="onegap">onegap - counts a string of gaps as a single gap</option>
   <option value="nogaps">nogaps - ignores gaps</option>
   <option value="eachgap ">eachgap - penalize each gap</option>
  </param>
  <param name="countends" type="boolean" checked="true" truevalue="" falsevalue="--countends=false" label="countends - Penalize terminal gaps"/>
  <param name="cutoff" type="float" value="0.0" label="cutoff - Distance Cutoff threshold - ignored if not > 0" 
                 help="Do not save any distances larger than this, a common value would be 0.10"/>
  <param name="output" type="select" label="output - Distance Matrix Output Format" help="">
   <option value="">Default Column-Formatted Matrix</option>
   <option value="lt">Phylip formatted Lower Triangle Matrix</option>
   <option value="square">Phylip formatted Square Matrix</option>
  </param>
  <!--
   oldfasta
   column
  -->
 </inputs>
 <outputs>
  <data format="html" name="logfile" label="${tool.name} on ${on_string}: logfile" />
  <data format="pair.dist" name="out_dist" label="${tool.name} on ${on_string}: dist">
   <change_format>
    <when input="output" value="lt" format="lower.dist" />
    <when input="output" value="square" format="square.dist" />
   </change_format>
  </data>
 </outputs>
 <requirements>
  <requirement type="binary">mothur</requirement>
 </requirements>
 <tests>
 </tests>
 <help>
**Mothur Overview**

Mothur_, initiated by Dr. Patrick Schloss and his software development team
in the Department of Microbiology and Immunology at The University of Michigan,
provides bioinformatics for the microbial ecology community.

.. _Mothur: http://www.mothur.org/wiki/Main_Page

**Command Documenation**

The dist.seqs_ command will calculate uncorrected pairwise distances between aligned sequences.  The command will generate a column-formatted_distance_matrix_ that is compatible with the column option in the read.dist command. The command is also able to generate a phylip-formatted_distance_matrix_. There are several options for how to handle gap comparisons and terminal gaps. 

.. _column-formatted_distance_matrix: http://www.mothur.org/wiki/Column-formatted_distance_matrix
.. _phylip-formatted_distance_matrix: http://www.mothur.org/wiki/Phylip-formatted_distance_matrix
.. _dist.seqs: http://www.mothur.org/wiki/Dist.seqs


 </help>
</tool>