# HG changeset patch # User Jim Johnson # Date 1370464677 18000 # Node ID 06675bd664eeba028a8fe3a489cbc690303b815f # Parent f4eadbd2e7c1e217b3324986686c08838e15af09 Update to DeFuse verion 0.6.1 and change tool dependencies for autoinstall diff -r f4eadbd2e7c1 -r 06675bd664ee README --- a/README Fri Jan 18 14:56:40 2013 -0600 +++ b/README Wed Jun 05 15:37:57 2013 -0500 @@ -1,4 +1,4 @@ -The DeFuse galaxy tool is based on DeFuse_Version_0.6.0 +The DeFuse galaxy tool is based on DeFuse_Version_0.6.1 http://sourceforge.net/apps/mediawiki/defuse/index.php?title=Main_Page DeFuse is a software package for gene fusion discovery using RNA-Seq data. The software uses clusters of discordant paired end alignments to inform a split read alignment analysis for finding fusion boundaries. The software also employs a number of heuristic filters in an attempt to reduce the number of false positives and produces a fully annotated output for each predicted fusion. @@ -35,7 +35,9 @@ These datasets should be referenced in the tool-data/defuse.loc file. -External Tools ( http://sourceforge.net/apps/mediawiki/defuse/index.php?title=DeFuse_Version_0.6.0 ) +Galaxy will try to auto-install dependencies: + +External Tools ( http://sourceforge.net/apps/mediawiki/defuse/index.php?title=DeFuse_Version_0.6.1 ) deFuse relies on other publically available tools as part of its pipeline. Some of these tools are not included with the deFuse download. Obtain these tools as detailed below. Download samtools The latest version of samtools can be downloaded from sourceforge: https://sourceforge.net/projects/samtools/files/samtools. diff -r f4eadbd2e7c1 -r 06675bd664ee defuse.xml --- a/defuse.xml Fri Jan 18 14:56:40 2013 -0600 +++ b/defuse.xml Wed Jun 05 15:37:57 2013 -0500 @@ -1,12 +1,11 @@ - + identify fusion transcripts - defuse + defuse samtools - bowtie - gmap - blat - fatotwobit + bowtie + gmap + kent /bin/bash $shscript diff -r f4eadbd2e7c1 -r 06675bd664ee repository_dependencies.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/repository_dependencies.xml Wed Jun 05 15:37:57 2013 -0500 @@ -0,0 +1,7 @@ + + + + + + + diff -r f4eadbd2e7c1 -r 06675bd664ee tool_dependencies.xml --- a/tool_dependencies.xml Fri Jan 18 14:56:40 2013 -0600 +++ b/tool_dependencies.xml Wed Jun 05 15:37:57 2013 -0500 @@ -1,10 +1,31 @@ - + + + + + + + + + + + + + + + + - http://sourceforge.net/projects/defuse/files/defuse/0.6/defuse-0.6.0.tar.gz - cd tools && make + http://sourceforge.net/projects/defuse/files/defuse/0.6/defuse-0.6.1.tar.gz + + + + + + + export CPLUS_INCLUDE_PATH=$BOOST_ROOT_DIR:$CPLUS_INCLUDE_PATH && cd tools && make . $INSTALL_DIR @@ -20,161 +41,4 @@ - - - - http://sourceforge.net/projects/samtools/files/samtools/0.1.18/samtools-0.1.18.tar.bz2 - sed -i.bak -e 's/-lcurses/-lncurses/g' Makefile - make - - samtools - $INSTALL_DIR/bin - - - misc/maq2sam-long - $INSTALL_DIR/bin - - - $INSTALL_DIR/bin - - - - -Compiling SAMtools requires the ncurses and zlib development libraries. - - - - - - - - http://downloads.sourceforge.net/project/bowtie-bio/bowtie/0.12.7/bowtie-0.12.7-src.zip - make - - bowtie - $INSTALL_DIR/bin - - - bowtie-build - $INSTALL_DIR/bin - - - bowtie-inspect - $INSTALL_DIR/bin - - - $INSTALL_DIR/bin - - - - - Compiling Bowtie requires libpthread to be present on your system. - - - - - - - http://research-pub.gene.com/gmap/src/gmap-gsnap-2012-07-20.v2.tar.gz - ./configure - make - - src/gmap - $INSTALL_DIR/bin - - - src/gmapindex - $INSTALL_DIR/bin - - - src/gsnap - $INSTALL_DIR/bin - - - src/uniqscan - $INSTALL_DIR/bin - - - src/iit_store - $INSTALL_DIR/bin - - - src/iit_get - $INSTALL_DIR/bin - - - src/atoiindex - $INSTALL_DIR/bin - - - src/snpindex - $INSTALL_DIR/bin - - - src/cmetindex - $INSTALL_DIR/bin - - - src/get-genome - $INSTALL_DIR/bin - - - util - $INSTALL_DIR/bin - - - $INSTALL_DIR/bin - - - - - - - - - - - http://hgdownload.cse.ucsc.edu/admin/exe/linux.x86_64/blat/blat - chmod 755 blat - - blat - $INSTALL_DIR/bin - - - $INSTALL_DIR/bin - - - - -This only handles blat for a non-commercial linux system. - -Please note that the Blat source and executables are freely available for -academic, nonprofit and personal use. Commercial licensing information is -available on the Kent Informatics website (http://www.kentinformatics.com/). - - - - - - - http://hgdownload.cse.ucsc.edu/admin/exe/linux.x86_64/faToTwoBit - chmod 755 faToTwoBit - - faToTwoBit - $INSTALL_DIR/bin - - - $INSTALL_DIR/bin - - - - -This only handles faToTwoBit for a non-commercial linux system. - -Please note that the source and executables are freely available for -academic, nonprofit and personal use. Commercial licensing information is -available on the Kent Informatics website (http://www.kentinformatics.com/). - - -