Mercurial > repos > jjohnson > cistrome_ceas
view heatmapr.xml @ 4:e426a7a39562 default tip
Set version on Conservation Plot tool
author | Jim Johnson <jj@umn.edu> |
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date | Wed, 17 Dec 2014 15:11:38 -0600 |
parents | 0476ee45d253 |
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<tool name="Heatmap" id="ceas_heatmapr" version="0.1.0"> <description>Draw the scores heatmap near a given interval set and use k-means to cluster the intervals</description> <macros> <import>ceas_macros.xml</import> </macros> <expand macro="requirements_bx" /> <command> #set $wfiles = str($wfile) #set $ttitle = "\""+str($title)+"\"" #set $txlabel = "\""+str($xlabel)+"\"" #set $tylabel = "\""+str($ylabel)+"\"" #for $m in $more #set $wfiles = $wfiles + "," + str($m.wfile) ###set $ttitle = $ttitle + ",\"" + str($m.title)+"\"" #set $txlabel = $txlabel + ",\"" + str($m.xlabel)+"\"" #set $tylabel = $tylabel + ",\"" + str($m.ylabel)+"\"" #end for #set $advparam = " --title="+str($ttitle)+" --subtitle="+str($txlabel)+" --y_label="+str($tylabel) #if $methodadvopt.methodadvopt_select == "kmeans" #set $methodopt = " --method="+str($methodadvopt.methodadvopt_select)+" -k "+str($methodadvopt.kmeans)+" --k_wigindex="+str($methodadvopt.k_wigindex) #else if $methodadvopt.methodadvopt_select == "none" #set $methodopt = " --method="+str($methodadvopt.methodadvopt_select) #else #set $methodopt = " --method="+str($methodadvopt.methodadvopt_select)+" --s_wigindex="+str($methodadvopt.s_wigindex) #end if #if $advopt.advopt_select == "yes" #set $advparam = $advparam+" -s "+str($advopt.saturation)+" --upstream="+str($advopt.upstrm)+" --downstream="+str($advopt.dnstrm)+" --pf-res="+str($advopt.step)+" --fontsize="+str($advopt.fontsize)+" --col="+str($advopt.colors)+" --pic_width="+str($advopt.pic_width)+" --pic_height="+str($advopt.pic_height)+" "+str($advopt.horizontal_line)+" "+str($advopt.vertical_line)+" "+str($advopt.dir)+" "+str($advopt.set_seed) #if $advopt.zmax.value #set $advparam = $advparam+" --zmax="+str($advopt.zmax) #end if #if $advopt.zmin.value #set $advparam = $advparam+" --zmin="+str($advopt.zmin) #end if #else #set $advparam = " " #end if ## heatmapr -b "$bfile" -w "$wfiles" #if $wfile.extension == "wig" --wig_format="wig" #elif $wfile.extension == "bigwig" --wig_format="bigwig" #end if --name=$name $methodopt $advparam &> $log && Rscript output_kmeans.r </command> <inputs> <param name="name" type="hidden" value="output"/> <param format="bed" name="bfile" type="data" label="BED file(center will be aligned, 100,000 lines max)" /> <param format="wig,bigwig" name="wfile" type="data" label="wig / bigwig file" /> <param name="title" type="text" label="Title for the whole heatmap plot" value="Heatmap" /> <param name="xlabel" type="text" label="Subtitle for heatmap plot" value="subtitle" /> <param name="ylabel" type="text" label="Y-label for heatmap plot" value="Y-label" /> <repeat name="more" title="More wig / bigwig files"> <param format="wig,bigwig" name="wfile" type="data" label="WIGGLE file" /> <param name="xlabel" type="text" label="Subtitle for heatmap plot" value="subtitle" /> <param name="ylabel" type="text" label="Y-label for heatmap plot" value="Y-label" /> </repeat> <conditional name="methodadvopt"> <param name="methodadvopt_select" type="select" label="choose sort/clustering method"> <option value="kmeans">cluster - kmeans</option> <option value="median">only sort - median</option> <option value="maximum">only sort - maximun</option> <option value="mean">only sort - mean</option> <option value="none">none - keep original order of bed.</option> </param> <when value="kmeans"> <param name="kmeans" type="integer" label="K-means number" value="5"> <validator type="in_range" max="10" min="3" message="K-means is out of range, K-means has to be between 3 to 10" /></param> <param name="k_wigindex" type="text" label="use which wigs to calc kmeans. input 'all' or sth like '1,2,4' (use the 1st, 2nd and 4th wig.)" value="all" /> </when> <when value="median"> <param name="s_wigindex" type="integer" label="key wig file for sorting" value="1" /> </when> <when value="maximum"> <param name="s_wigindex" type="integer" label="key wig file for sorting" value="1" /> </when> <when value="mean"> <param name="s_wigindex" type="integer" label="key wig file for sorting" value="1" /> </when> </conditional> <conditional name="advopt"> <param name="advopt_select" type="select" label="Advanced Options"> <option value="no">No</option> <option value="yes">Yes</option> </param> <when value="yes"> <param name="step" type="integer" label="Step" value="10"> <validator type="in_range" max="1000" min="5" message="step is out of range, step has to be between 5 to 1000" /> </param> <param name="saturation" type="float" label="Saturation" value="0.01" /> <param name="upstrm" type="integer" label="Upstream" value="500"> <validator type="in_range" max="10000" min="100" message="Upstream is out of range, Upstream has to be between 100 to 10000" /> </param> <param name="dnstrm" type="integer" label="Downstream" value="500"> <validator type="in_range" max="10000" min="100" message="Downstream is out of range, Downstream has to be between 100 to 10000" /> </param> <param name="fontsize" type="integer" label="Image fontsize. (better not change)" value="1" /> <param name="colors" type="text" size="100" label="colorRamp for the legend of heatmap, from low value to high, use ','to split" value="053061,2166AC,4393C3,92C5DE,D1E5F0,F7F7F7,FDDBC7,F4A582,D6604D,B2182B,67001F" /> <param name="pic_width" type="integer" label="Heatmap width" value="1920"> <validator type="in_range" max="16000" min="1600" message="Heatmap wodth is out of range, width has to be between 1600 to 16000" /> </param> <param name="pic_height" type="integer" label="Heatmap height" value="1440"> <validator type="in_range" max="12000" min="1200" message="Heatmap height is out of range, height has to be between 1200 to 12000" /> </param> <param name="zmin" type="text" label="minimum value in image" /> <param name="zmax" type="text" label="maximum value in image" /> <param name="horizontal_line" type="boolean" label="Plot classified lines?" checked="no" truevalue="-z" falsevalue=" " /> <param name="vertical_line" type="boolean" label="Plot vertical line?" checked="no" truevalue="-v" falsevalue=" " /> <param name="dir" type="boolean" label="consider the direction (+/-) while profiling" checked="no" truevalue="--dir" falsevalue=" " /> <param name="set_seed" type="boolean" label="Set seed to make result reproducible (for developers)" checked="no" truevalue="--set-seed" falsevalue=" " /> </when> <when value="no" /> </conditional> </inputs> <outputs> <data format="png" name="outputpng" label="Heatmap image" from_work_dir="output_r.heatmap.png"/> <data format="txt" name="outputr" label="Heatmapr R script" from_work_dir="output_kmeans.r"/> <data format="tabular" name="outputbed" label="Heatmap k-means classified regions" from_work_dir="output_peak_classid"/> <data format="txt" name="log" label="Heatmap log" /> </outputs> <expand macro="stdio" /> <tests> <test> <param name="wfile" value="treatment.wig" /> <param name="bfile" value="peak.bed" /> <param name="title" value="title" /> <param name="xlabel" value="xlabel" /> <param name="ylabel" value="ylabel" /> <param name="methodadvopt_select" value="kmeans" /> <param name="kmeans" value="5" /> <param name="advopt_select" value="yes" /> <param name="k_wigindex" value="1" /> <param name="step" value="10" /> <param name="upstrm" value="500" /> <param name="dnstrm" value="400" /> <param name="fontsize" value="2" /> <param name="colors" value="053061,2166AC,4393C3,92C5DE,D1E5F0,F7F7F7,FDDBC7,F4A582,D6604D,B2182B,67001F" /> <param name="zmin" value="" /> <param name="zmax" value="" /> <param name="pic_width" value="1600" /> <param name="pic_height" value="1200" /> <param name="horizontal_line" value="-z" /> <param name="vertical_line" value="-v" /> <param name="dir" value=" " /> <param name="set_seed" value="--set-seed" /> <output name="outputbed"> <assert_contents> <has_text_matching expression="1\tchr19\t58470227\t58470701\tMACS_peak_9625\t113.34" /> </assert_contents> </output> </test> </tests> <help> This tool draws the scores heatmap near a given interval set..It's based on sitrpro script in Hyunjin Shin's library. Original code is written by Jian Ma. It uses R module for kmeans clustering and draw the heatmap. .. class:: warningmark **NEED IMPROVEMENT** ----- **Parameters** - **BED file** contains the regions to extract the scores from WIGGLE - **K-means (Advanced)** defines the number of classes in k-means clustering. files. - **WIGGLE file** contains the scores for heatmap. - **More WIGGLE file** click the *Add new More WIGGLE files* to add more. All the heatmap for the WIGGLE files will be aligned and clutered together. - **Advanced Options** to turn on advanced settings. You will lose advanced settings and go back to default parameters when you choose 'No'. - **Upstream/Downstream (Advanced)** size around regions in BED file. - **Step (Advanced)** is the profiling resolution in bps. - **Saturation** before the script use the score data to draw heatmap, it will saturate the data to a narrow scale. For example, if you set the option to 0.1, The data smaller than 10% of the data or bigger than 90% of the data will be saturated to 10% or 90% of the data. - **col_low/col_high** is the color of legend for images. - **pic_width/pic_height** is the size of heatmap image. - **zmin/zmax** is the max value and min value for the legend bar. - **horizontal_line** lines in black to separate the clusters. ----- **Outputs** - *PNG file* is the heatmap image - *clustering result file* Contain the clustering results from the tool. The 'Score' column (5th column) contains the cluster indices. - *Log* for job log. If you see errors, please attached this in the bug report </help> </tool>