Mercurial > repos > jjohnson > bamtools_split
diff bamtools-split-paired.xml @ 0:8c17ddca0eee draft
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author | jjohnson |
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date | Mon, 15 May 2017 16:27:18 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bamtools-split-paired.xml Mon May 15 16:27:18 2017 -0400 @@ -0,0 +1,56 @@ +<tool id="bamSplitPaired" name="Split BAM by Paired/Single End" version="2.4.0"> + <description></description> + <requirements> + <requirement type="package" version="2.4.0">bamtools</requirement> + </requirements> + <command> + <![CDATA[ + ln -s '${input_bam}' 'localbam.bam' && + ln -s '${input_bam.metadata.bam_index}' 'localbam.bam.bai' && + bamtools split -paired + -in localbam.bam + -stub split_bam + ]]> + </command> + <inputs> + <param name="input_bam" type="data" format="bam" label="BAM dataset to split by single_end/paired_end"/> + </inputs> + <outputs> + <data format="bam" name="single" label="${input_bam.name} mapped" from_work_dir="split_bam.SINGLE_END.bam" /> + <data format="bam" name="paired" label="${input_bam.name} unmapped" from_work_dir="split_bam.PAIRED_END.bam" /> + </outputs> + <tests> + <test> + <param name="input_bam" ftype="bam" value="bamtools-input-paired.bam"/> + <output name="single" file="split_bam.SINGLE_END.bam" compare="sim_size" delta="200" /> + <output name="paired" file="split_bam.PAIRED_END.bam" compare="sim_size" delta="200" /> + </test> + </tests> + <help> +**What is does** + +BAMTools split is a utility for splitting BAM files. It is based on BAMtools suite of tools by Derek Barnett (https://github.com/pezmaster31/bamtools). + +----- + +.. class:: warningmark + + +**How it works** + + +Splits the input BAM file into 2 output files named (SINGLE_END) and (PAIRED_END) containing single_end and paired_end reads, respectively. + +----- + +.. class:: infomark + +**More information** + +Additional information about BAMtools can be found at https://github.com/pezmaster31/bamtools/wiki + + </help> + <citations> + <citation type="doi">10.1093/bioinformatics/btr174</citation> + </citations> +</tool>