# HG changeset patch
# User jfb
# Date 1519335460 18000
# Node ID c39370503745768a3ea3a519093eb772ca7c612c
# Parent  e0fa918f22c79d2c6b709cf64b25bee5add8c040
Update to the xml to explain the purpose and use of this tool

diff -r e0fa918f22c7 -r c39370503745 zippable Kinamine-JJ/Kinamine.xml
--- a/zippable Kinamine-JJ/Kinamine.xml	Thu Feb 22 15:38:48 2018 -0500
+++ b/zippable Kinamine-JJ/Kinamine.xml	Thu Feb 22 16:37:40 2018 -0500
@@ -31,17 +31,26 @@
 
     
     <help><![CDATA[
-This takes a Distinct Peptide Report and extracts from it all phospho-motifs that were discovered at a threshold above a given FDR value.  This tool is intended to be used in conjunction with Negative Motif Finder and Kinatest.R, the three together creating a GalaxyP version of the KINATEST-ID workbook.
+Kinamine takes a Distinct Peptide Report and extracts from it all peptides containing a phospho(Y), along with the 7 amino acids N- and C- terminal to the pY in that peptide (assuming they were discovered at a threshold above the given FDR value).  This file is the *Substrates* file
+Additionally, all discovered peptides are assumed to have come from a protein.  For every phospho(Y) peptide, KinaMine takes the associated protein accession number, searches the FASTA file for that accession number and returns the amino acid percent composition of the protein(s) which that peptide came from.  This file is the *Substrate Background Frequency* file
+This tool is intended to be used in conjunction with Negative Motif Finder and Kinatest.R, the three together creating a GalaxyP version of the KINATEST-ID workbook.
 
 ===========
 Inputs
 ===========
 **Distinct Peptide Report**
-This is... ask Minervo
+This is a the Distinct Peptide Summary of a Protein Pilot search (though the summary must be exported as a .txt file in this case).  The Distinct Peptide Summary is one of the tabs in .xlsx file created by Protein Pilot, it can be exported to a .txt file by normal excel functions
 
 **FASTA reference**
 This should be a FASTA taken from the Reviewed Human Uniprot database, consisting of all human proteins plus a list of common Mass Spectrometry contaminants
 
+**Intended Purpose**
+This tool is intended for Academic use.  
+This tool is intended as part of a KALIP-KINATEST pipeline.  
+Briefly, KALIP involved treating cell lysate first with trypsin, second with phosphotase, and finally with a given kinase, then running that cell lysate on a Mass Spec to discover what peptides were present.  
+KinaMine then extracts only the phospho-Y peptides and the proteins they came from.  Negative Motif finder finds all pY-containing peptides that could have been discovered but weren't.  Kinatest then uses all this data to discover candidate substrates which will be specific for the given kinase and no others.  From the discovered substrates, a biosensor can be made.
+
+
     ]]></help>
     <citations>
         <citation type="doi">10.1021/ja507164a</citation>