view frag_lens @ 1:20d941ecc1bb draft default tip

"planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/b2b_utils commit 9bf8a0462bd44f170c0371b6cae67dd0c3b3da9f-dirty"

#!/usr/bin/env perl

use strict;
use warnings;
use 5.012;

use Cwd qw/abs_path/;
use File::Temp qw/tempdir tempfile/;
use IPC::Cmd qw/can_run/;
use List::Util qw/sum/;
use Getopt::Long;
use Pod::Usage;

my @good_codes = ( 0x0002, 0x0040 );
my @bad_codes  = ( 0x0004, 0x0100, 0x0800 );

#-inputs---------------------------------------------------------------------#
my $fasta;
my $forward;
my $reverse;
my $sam;
#-knobs----------------------------------------------------------------------#
my $threads = 1;
my $max_align = 10000;

my $PROGRAM = 'frag_lens';
my $VERSION = 0.001;

GetOptions(
    #-inputs-----------------------------------------------------------------#
    'sam=s'     => \$sam,
    'forward=s' => \$forward,
    'reverse=s' => \$reverse,
    'ref=s'     => \$fasta,
    #-knobs------------------------------------------------------------------#
    'threads=i' => \$threads,
    'max_aln=i' => \$max_align,
    'help'      => sub{ pod2usage(-verbose => 2); },
    'version'   => sub{ say "This is $PROGRAM v$VERSION";exit; },

) or pod2usage( -verbose => 1);

my $fh_sam;
my $tmp_fasta;

if (defined $sam) {
    open $fh_sam, '<', $sam or die "failed to open SAM\n";
}

else {

    my $BWA = can_run('bwa')
        // die "BWA is required but not found\n";

    my ($tmp_dir) = tempdir( CLEANUP => 1);

    die "specify forward and reverse read files and reference\n"
        if (! defined $forward || ! defined $reverse || ! defined $fasta); 

    $fasta = abs_path($fasta);
    
    my $res = system(
        'ln',
        '-s',
        $fasta,
        "$tmp_dir/tmp.fasta"
    );
    die "link failed" if ($res);
    $res = system(
        $BWA,
        'index',
        "$tmp_dir/tmp.fasta"
    );
    die "index failed" if ($res);
    open $fh_sam, '-|', $BWA,
        'mem',
        '-t' => $threads,
        '-v' => 1,
        "$tmp_dir/tmp.fasta",
        $forward,
        $reverse
    ;
}

my $c = 0;
while (my $line = <$fh_sam>) {
    next if ($line =~ /^\@/);
    chomp $line;
    my @parts = split "\t", $line;
    my $flags = $parts[1];
    my $sum1 = sum map {$_ & $flags ? 1 : 0} @good_codes;
    my $sum2 = sum map {$_ & $flags ? 1 : 0} @bad_codes;
    if ($sum1 == scalar @good_codes && $sum2 == 0) {
        say abs($parts[8]);
        last if (++$c >= $max_align);
    }
}
close $fh_sam;

__END__

=head1 NAME

frag_lens - Calculate paired end fragment lengths from read alignment

=head1 SYNOPSIS

frag_lens [--sam <in.sam>] OR [--ref <cons.fa> --forward <R1.fq> --reverse <R2.fq>] [options] > frag_lens.txt

=head1 DESCRIPTION

Calculates library fragment lengths based on paired-end read alignment.
Takes as input either a preprepared SAM alignment or a reference and read
files from which it produces an alignment. Outputs calculated fragment
lengths, one per line.

=head1 PREREQUISITES

Requires the following binaries:

=over 1

=item * bwa

=back

=head1 OPTIONS

=head2 Input option one

=over 4

=item B<--sam> I<filename>

Path to input SAM alignment.

=back

=head2 Input option two

=over 4

=item B<--ref> I<filename>

Path to reference sequence (e.g. assembly)

=item B<--forward> I<filename>

Forward reads in FASTQ format

=item B<--reverse> I<filename>

Reverse reads in FASTQ format

=back

=head2 Configuration 

=over 4

=item B<--max_align>

Maximum number of alignment records to read as input. Used to limit run times.

=item B<--threads>

Number of threads to use for alignment (ignored if --sam is given)

=back

=head1 CAVEATS AND BUGS

Please submit bug reports to the issue tracker in the distribution repository.

=head1 AUTHOR

Jeremy Volkening ([e-mail])

=head1 LICENSE AND COPYRIGHT

Copyright 2014-19 Jeremy Volkening

This program is free software: you can redistribute it and/or modify
it under the terms of the GNU General Public License as published by
the Free Software Foundation, either version 3 of the License, or
(at your option) any later version.

This program is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
GNU General Public License for more details.

You should have received a copy of the GNU General Public License
along with this program.  If not, see <http://www.gnu.org/licenses/>.

=cut