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comparison annotatePeaks.pl @ 4:82b10f23f1fe draft

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author jbrayet
date Thu, 13 Aug 2015 11:02:47 -0400
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3:db5076a0494f 4:82b10f23f1fe
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51 #
52 # Copyleft ↄ⃝ 2012 Institut Curie
53 # Author(s): Valentina Boeva, Alban Lermine (Institut Curie) 2012
54 # Contact: valentina.boeva@curie.fr, alban.lermine@curie.fr
55 # This software is distributed under the terms of the GNU General
56 # Public License, either Version 2, June 1991 or Version 3, June 2007.
57
58 #!/usr/bin/perl -w
59
60 #for a complete list of genes and a list of sites, sorts genes close to sites
61 #printDistance
62
63 #read only gene files with refSeqGenes
64 #counts only once overlapping transcripts or genes
65
66 #calculates some stats about locations of peaks
67
68 #uses hierarchie : promoter, imUpstream, intragenic,enh, 5kbdownstream, intergenic + for genes: fExon,exon,fIntron,intron,junction+-50kb ONLY FOR "findClosestGene"
69 #outputs fold change of expression is available
70
71 #outputname - corrected
72 #all isoforms are considered, even those that start and end at the same coordinates
73 #uses fluorescence values
74
75 #only one location per gene in mode "all" (but promoter+intragenic if both)
76
77 #read directly Noresp/down/up genes
78
79 #prints distance to TE
80
81 use strict;
82 use POSIX;
83
84 my $SitesFilename ;
85 my $GenesFilename ;
86 my $SelectedGenesFilename = "";
87 my $MirFilename = "";
88 my $minScore = 0;
89 my $BIGNUMBER = 10000000000;
90 my $enhLeft = -30000;
91 my $enhRight = -1500;
92 my $promLeft = $enhRight ;
93 my $immediateDownstream = 2000;
94 my $maxScore = $BIGNUMBER ;
95 my $verbose = 0;
96 my $maxDistance = $BIGNUMBER ;
97 my $downStreamDist = 5000;
98 my $jonctionSize = 50;
99 my $all = 0;
100 my $fluoFile = "";
101 my $regType = "";
102 my $GCislands = "";
103 my $ResFilename = "";
104
105 my $usage = qq{
106 $0
107
108 -----------------------------
109 mandatory parameters:
110
111 -g filename file with all genes
112 -tf filename file with sites of TF
113
114 -----------------------------
115 optional parameters:
116 -v for verbose
117 -mir filename file with positions of miRNA
118 -maxDist value maximal Distance to genes (def. Inf)
119 -minScore value minimal Score (def. 0)
120 -maxScore value maximal Score (def. Inf)
121 -selG filename file with selected genes and their expression levels
122 -all to output all genes intersecting with the peak
123 -fluo filename file with fluorescence
124 -regType valeu down-regulated, up-regulated, no-response
125 -gc filename file with gc-islands
126
127 -lp valeu upstream of TSS region to define promoter
128 -rp valeu downstream of TSS region to define immediate downstream
129 -enh valeu upstream of TSS region to define enhancer
130 -dg valeu downstream of transcription end region to define gene downstream
131
132 -o filename output filename
133
134 };
135
136 if(scalar(@ARGV) == 0){
137 print $usage;
138 exit(0);
139 }
140
141
142 while(scalar(@ARGV) > 0){
143 my $this_arg = shift @ARGV;
144 if ( $this_arg eq '-h') {print "$usage\n"; exit; }
145
146 elsif ( $this_arg eq '-g') {$GenesFilename = shift @ARGV;}
147 elsif ( $this_arg eq '-tf') {$SitesFilename = shift @ARGV;}
148 elsif ( $this_arg eq '-mir') {$MirFilename = shift @ARGV;}
149 elsif ( $this_arg eq '-selG') {$SelectedGenesFilename = shift @ARGV;}
150 elsif ( $this_arg eq '-maxDist') {$maxDistance = 1;}
151 elsif ( $this_arg eq '-maxScore') {$maxScore = shift @ARGV;}
152 elsif ( $this_arg eq '-minScore') {$minScore = shift @ARGV;}
153 elsif ( $this_arg eq '-v') {$verbose = 1;}
154 elsif ( $this_arg eq '-all') {$all = 1;}
155 elsif ( $this_arg eq '-regType') {$regType = shift @ARGV;}
156
157 elsif ( $this_arg eq '-fluo') {$fluoFile = shift @ARGV;}
158 elsif ( $this_arg eq '-gc') {$GCislands = shift @ARGV;}
159 elsif ( $this_arg eq '-o') {$ResFilename = shift @ARGV;}
160
161 elsif ( $this_arg eq '-lp') {$enhRight = shift @ARGV;$promLeft = $enhRight;}
162 elsif ( $this_arg eq '-rp') {$immediateDownstream = shift @ARGV;}
163 elsif ( $this_arg eq '-enh') {$enhLeft = shift @ARGV;}
164 elsif ( $this_arg eq '-dg') {$downStreamDist = shift @ARGV;}
165
166 elsif ( $this_arg =~ m/^-/ ) { print "unknown flag: $this_arg\n";}
167 }
168
169 if ($maxDistance<0) {
170 die "\tThe maximal distance must be positive!\n";
171 }
172 my $tmpn = $SitesFilename;
173 $tmpn =~ s/.*[\/\\]//;
174 if ($ResFilename eq "") {
175 $ResFilename = $SelectedGenesFilename.$tmpn."_".$minScore."_dists.txt";
176
177 if ($maxScore != $BIGNUMBER) {
178 $ResFilename = $SelectedGenesFilename.$SitesFilename."_".$minScore."_$maxScore"."_dists.txt";
179 }
180
181 if ($all == 1) {
182 $ResFilename =~ s/_dists/_all_dists/;
183 }
184 }
185
186
187 #-----------read selected genes----------------
188 my %selectedGenes;
189 my %selectedGenesFoldChange;
190 if ( $SelectedGenesFilename ne "") {
191 open (FILE, "<$SelectedGenesFilename ") or die "Cannot open file $SelectedGenesFilename !!!!: $!";
192 while (<FILE>) {
193 chomp;
194 my @a = split/\t/;
195 $selectedGenes{$a[1]} = $a[3];
196 $selectedGenesFoldChange{$a[1]} = $a[2];
197 #print "gene:$a[1],reg:$selectedGenes{$a[1]},FC:$selectedGenesFoldChange{$a[1]}\n";
198 }
199
200 close FILE;
201 print "\t\t$SelectedGenesFilename is read!\n" if ($verbose);
202 }
203
204 #-----------read genes with fluorescence---------
205 my %fluoGenes;
206 if ( $fluoFile ne "") {
207 open (FILE, "<$fluoFile ") or die "Cannot open file $fluoFile !!!!: $!";
208 my $gene = "";
209 my $med = 0;
210 my %h;
211 while (<FILE>) {
212 chomp;
213 my @a = split/\t/;
214
215 next if (scalar(@a)<5);
216 next if ($a[0] eq "probesets");
217 next unless ($a[0] =~m/\S/);
218 next unless ($a[4] =~m/\S/);
219 if ($gene ne "" && $gene ne $a[2]) {
220 #calcMed
221 $med = med(keys %h);
222 $fluoGenes{$gene} = $med;
223 $med=0;
224 delete @h{keys %h};
225 } else {
226 #$h{$a[4]} = 1;
227 }
228 $gene = $a[2];
229 $h{$a[4]} = 1;
230 #print "keys ", scalar(keys %h),"\t",keys %h,"\n";
231 }
232 if ($gene ne "") {
233 $med = med(keys %h);
234 $fluoGenes{$gene} = $med;
235 }
236 close FILE;
237 print "\t\t$fluoFile is read!\n" if ($verbose);
238 }
239 #-----------read GC-islands----------------
240 my %GCislands;
241 if ($GCislands ne "") {
242 open (FILE, "<$GCislands ") or die "Cannot open file $GCislands !!!!: $!";
243
244 while (<FILE>) {
245 chomp;
246 my @a = split/\t/;
247 #bin chrom chromStart chromEnd name length cpgNum gcNum perCpg perGc obsExp
248 #107 chr1 36568608 36569851 CpG: 128 1243 128 766 20.6 61.6 1.09
249 my $chr = $a[1];
250 my $start = $a[2];
251 my $end = $a[3];
252 $GCislands{$chr}->{$start}=$end;
253 }
254 close FILE;
255 } elsif ($verbose) {
256 print "you did not specify a file with GC-islands\n";
257 }
258
259 #-----------read genes----------------
260
261 my %genes;
262
263 my $count = 0;
264
265 open (GENES, "<$GenesFilename") or die "Cannot open file $GenesFilename!!!!: $!";
266 <GENES>;
267 while (<GENES>) {
268 chomp;
269 if (/(chr.*)\s([+-])\s(\d+)\s(\d+)\s(\d+)\s(\d+)\s(\d+)\s(\S+)\s(\S+)\s\S+\s(\S+)/){
270 my $name = $10;
271 my $chr = $1;
272 my $strand = $2;
273 if ($strand eq '+') {
274 $strand = 1;
275 }
276 else {
277 $strand = -1;
278 }
279 my $leftPos = $3;
280 my $rightPos = $4;
281 my $cdsStart= $5;
282 my $cdsEnd= $6;
283 my $exonCount= $7;
284 my $exonStarts= $8;
285 my $exonEnds= $9;
286 my $ID = "$name\t$chr:$leftPos-$rightPos;$exonStarts-$exonEnds";
287 my $foldChange = 1;
288 my $reg = "NA";
289 my $fluo = "NA";
290 if ( $SelectedGenesFilename ne "") {
291 #print "$name\t";
292 if (exists($selectedGenes{$name})) {
293 $reg = $selectedGenes{$name};
294 $foldChange = $selectedGenesFoldChange{$name};
295 }
296 }
297 unless (exists($genes{$chr})) {
298 my %h;
299 $genes{$chr} = \%h;
300 }
301 unless (exists($genes{$chr}->{$ID})) {
302 my %h1;
303 $genes{$chr}->{$ID} = \%h1;
304 $count++;
305 }
306 if ( $fluoFile ne "") {
307 if (exists($fluoGenes{$name})) {
308 $fluo = $fluoGenes{$name};
309 }
310 }
311 $genes{$chr}->{$ID}{'name'} = $name ;
312 $genes{$chr}->{$ID}{'left'} = $leftPos ;
313 $genes{$chr}->{$ID}{'right'} = $rightPos ;
314 $genes{$chr}->{$ID}{'cdsStart'} = $cdsStart;
315 $genes{$chr}->{$ID}{'cdsEnd'} = $cdsEnd;
316 $genes{$chr}->{$ID}{'strand'} = $strand;
317 $genes{$chr}->{$ID}{'exonCount'} = $exonCount;
318 $genes{$chr}->{$ID}{'exonStarts'} = $exonStarts;
319 $genes{$chr}->{$ID}{'exonEnds'} = $exonEnds;
320 $genes{$chr}->{$ID}{'TSS'} = ($strand == 1) ? $leftPos :$rightPos ;
321 $genes{$chr}->{$ID}{'TE'} = ($strand == -1) ? $leftPos :$rightPos ;
322 $genes{$chr}->{$ID}{'reg'} = $reg;
323 $genes{$chr}->{$ID}{'foldChange'} = $foldChange;
324 $genes{$chr}->{$ID}{'length'} = abs ($leftPos-$rightPos);
325 ($genes{$chr}->{$ID}{'firstIntronStart'},$genes{$chr}->{$ID}{'firstIntronEnd'}) = getFirstIntron ($exonCount,$exonStarts,$exonEnds,$strand);
326 ($genes{$chr}->{$ID}{'firstExonStart'},$genes{$chr}->{$ID}{'firstExonEnd'}) = getFirstExon ($exonCount,$exonStarts,$exonEnds,$strand);
327
328 $genes{$chr}->{$ID}{'fluo'} = $fluo;
329
330 $genes{$chr}->{$ID}{'GCisland'} = 0;
331 if ($GCislands ne "") {
332 $genes{$chr}->{$ID}{'GCisland'} = checkIfGC ($genes{$chr}->{$ID}{'TSS'},$strand,2000,$GCislands{$chr});
333 }
334
335 }
336 }
337 print "Total genes : $count\n";
338 close GENES;
339 print "\t\t$GenesFilename is read!\n" if ($verbose);
340 #for my $gName (sort keys %{$genes{'chr18'}}) {
341
342 # print "$gName\t$genes{'chr18'}->{$gName}{'TSS'}\n";
343 #}
344
345 #-----------read file with sites miRNA, store as genes-----
346
347 if ( $MirFilename eq ""){
348 print "you did not specify file with miRNA\n" if ($verbose);;
349 }
350 else {
351
352
353 open (MIR, "<$MirFilename ") or die "Cannot open file $MirFilename !!!!: $!";
354 #chr1 20669090 20669163 mmu-mir-206 960 +
355 while (<MIR>) {
356 chomp;
357 my ($name, $chr, $leftPos, $rightPos, $strand );
358 my $fluo = "NA";
359
360 #1 . miRNA 20669091 20669163 . + . ACC="MI0000249"; ID="mmu-mir-206";
361 if (/([0-9XYM]+)\s.\smiRNA\s(\d+)\s(\d+)\s.\s([+-])\s.\sACC=.*ID=\"(.*)\"/) {
362 $name = $5;
363 $chr = $1;
364 $leftPos = $2;
365 $rightPos = $3;
366 $strand = $4;
367 }
368 elsif (/(.*)\s(\d+)\s(\d+)\s(.*)\s(.*)\s(.*)/){
369 $name = $4;
370 $chr = $1;
371 $leftPos = $2;
372 $rightPos = $3;
373 $strand = $6;
374 } else {
375 next;
376 }
377
378 unless ($chr =~ m/chr/) {
379 $chr = "chr".$chr;
380 }
381 my $ID = "$name\t$chr:$leftPos-$rightPos";
382
383 if ($strand eq '+') {
384 $strand = 1;
385 }
386 else {
387 $strand = -1;
388 }
389
390 unless (exists($genes{$chr})) {
391 my %h;
392 $genes{$chr} = \%h;
393 }
394 unless (exists($genes{$chr}->{$ID})) {
395 my %h1;
396 $genes{$chr}->{$ID} = \%h1;
397 $count++;
398 }
399 $genes{$chr}->{$ID}{'name'} = $name ;
400 $genes{$chr}->{$ID}{'left'} = $leftPos ;
401 $genes{$chr}->{$ID}{'right'} = $rightPos ;
402 $genes{$chr}->{$ID}{'cdsStart'} = $leftPos ;
403 $genes{$chr}->{$ID}{'cdsEnd'} = $rightPos ;
404 $genes{$chr}->{$ID}{'strand'} = $strand;
405 $genes{$chr}->{$ID}{'length'} = abs ($leftPos-$rightPos);
406 $genes{$chr}->{$ID}{'exonCount'} = 1;
407 $genes{$chr}->{$ID}{'exonStarts'} = $leftPos ;
408 $genes{$chr}->{$ID}{'exonEnds'} = $rightPos ;
409 $genes{$chr}->{$ID}{'TSS'} = ($strand == 1) ? $leftPos :$rightPos ;
410 $genes{$chr}->{$ID}{'TE'} = ($strand == -1) ? $leftPos :$rightPos ;
411 $genes{$chr}->{$ID}{'reg'} = "miRNA";
412 $genes{$chr}->{$ID}{'foldChange'} = 1;
413 ($genes{$chr}->{$ID}{'firstIntronStart'},$genes{$chr}->{$ID}{'firstIntronEnd'}) = (0,0);
414 ($genes{$chr}->{$ID}{'firstExonStart'},$genes{$chr}->{$ID}{'firstExonEnd'}) = (0,0);
415
416 $genes{$chr}->{$ID}{'fluo'} = $fluo;
417 $genes{$chr}->{$ID}{'GCisland'} = 0;
418
419
420
421 }
422
423
424 close MIR;
425 print "\t\t$MirFilename is read!\n" if ($verbose) ;
426 }
427 #-----------read file with sites and find N closest genes-----
428 my $numberOfAllSites = 0;
429
430 open (FILE, "<$SitesFilename") or die "Cannot open file $SitesFilename!!!!: $!";
431 open (OUT , ">$ResFilename") or die "Cannot open file $ResFilename!!!!: $!";
432 print OUT "Chromosome\tStart\tEnd\tMax\tScore\tDistTSS\tType\tTypeIntra\tReg\tFoldChange\tDistTE\tGeneName\tGeneCoordinates\n" ;
433 my $header = <FILE>; #read header
434 my @a = split (/\t/,$header );
435 my $correction = 0;
436 if ($a[1] =~m/chr/) {
437 $correction=1;
438 }
439
440 unless ($header=~m/Chromosome/ || $header=~m/track/|| $header=~m/Start/i) {
441 close FILE;
442 open (FILE, "<$SitesFilename") or die "Cannot open file $SitesFilename!!!!: $!";
443 }
444
445 $count = 0;
446
447 my %typehash;
448 my %typehashIntra;
449
450 $typehashIntra{"f_exon"} = 0;
451 $typehashIntra{"f_intron"} = 0;
452 $typehashIntra{"jonction"} = 0;
453 $typehashIntra{"exon"} = 0;
454 $typehashIntra{"intron"} = 0;
455
456
457 while (<FILE>) {
458 chomp;
459 $numberOfAllSites++;
460 my @a = split /\t/;
461 my $chro = $a[0+$correction];
462 my $fpos = $a[1+$correction];
463 my $lpos = $a[2+$correction];
464 my $maxpos = $a[3+$correction];
465 if ($maxpos =~ m/\D/) {
466 $maxpos = int(($lpos+$fpos)/2);
467 }
468 my $score = $a[4+$correction];
469 next if ($score < $minScore);
470 next if ($score >= $maxScore);
471 $score = $score +1 -1;
472 #print "$score" ;
473
474 #my $site = "$chro:$fpos-$lpos\t$maxpos\t$score";
475 #print $site ;
476 #my $motifNumber = $a[3];
477
478 #my $geneSet = &search_genes($N,$chro,($fpos+$lpos)/2,\%genes);
479 my @b;
480 unless ($all) {
481 @b = &findClosestGene($fpos,$lpos,$score,$chro,$maxpos,\%genes); #findAllClosestGene for all genes overlaping a peak
482 } else {
483 @b = &findAllClosestGeneOneLocation ($fpos,$lpos,$score,$chro,$maxpos,\%genes); #findAllClosestGene
484 }
485 #print "$distTSS\t$distTE\n" unless ($distTSS == $BIGNUMBER);
486 for my $type (@b) {
487 $typehash{$type} = 0 unless (exists($typehash{$type}));
488 $typehash{$type}++;
489 }
490 $count ++;
491
492 }
493 close FILE;
494 close OUT ;
495
496 print "Total Sites: $count\n";
497 my $nEntries = 0;
498 for my $type (sort keys %typehash) {
499 $nEntries += $typehash{$type};
500 }
501 print "Type\tCount\tFrequency\n";
502 for my $type (sort keys %typehash) {
503 print $type,"\t",$typehash{$type},"\t",$typehash{$type}/$nEntries,"\n";
504 }
505 for my $type (sort keys %typehashIntra) {
506 print $type,"\t",$typehashIntra{$type},"\t",$typehashIntra{$type}/$nEntries,"\n";
507 }
508
509
510 #my @arr = @{$genes{'chr'}};
511
512 sub findClosestGene {
513 my ($fpos,$lpos,$score,$chro,$pos,$genes) = @_;
514 my ($distTSS,$distTE,$type,$reg,$geneName,$foldChange) = ($BIGNUMBER,$BIGNUMBER,"intergenic",0,0,0);
515 my $locDistTSS = 0;
516 my $locDistTE = 0;
517 my %hashForOverlapingGenes;
518 my @array2return;
519 my $typeIntra = "NA";
520 for my $gName (keys %{$genes->{$chro}}) {
521 my $TSS = $genes->{$chro}{$gName}{'TSS'};
522 my $strand = $genes->{$chro}{$gName}{'strand'};
523 my $TE = $genes->{$chro}{$gName}{'TE'};
524
525 $locDistTE = ($pos-$TE)*$strand;
526 $locDistTSS = ($pos-$TSS)*$strand;
527 #print "$pos-$genes->{$chro}{$gName}{'TSS'})*$genes->{$chro}{$gName}{'strand'} = $locDistTSS\n";
528 #my $locDistTE = ($pos-$genes->{$chro}{$gName}{'TE'})*$genes->{$chro}{$gName}{'strand'};
529
530 #print "$gName $maxDistance $pos-$genes->{$chro}{$gName}{'TSS'} $genes->{$chro}{$gName}{'strand'}\n";
531 #print "$locDistTSS $enhLeft $enhRight\n";
532
533 if (($locDistTSS>= $enhLeft)&&($locDistTSS<$enhRight)) {
534 $type = "enhancer";
535 } elsif (($locDistTSS>= $enhRight)&&($locDistTSS<=0)) {
536 $type = "promoter";
537 } elsif (($locDistTSS> 0)&&($locDistTSS<=$immediateDownstream)&&($locDistTE<=0)) {
538 $type = "immediateDownstream";
539 } elsif (($locDistTSS >= $immediateDownstream)&&($locDistTE<=0)) {
540 $type = "intragenic";
541 } elsif (($locDistTE >= 0)&&($locDistTE<=$downStreamDist)) {
542 $type = "5kbDownstream";
543 } elsif (abs($locDistTSS)<abs($distTSS)) {
544 $distTSS = $locDistTSS;
545 $distTE = $locDistTE;
546 ($reg,$geneName,$foldChange) = ($genes->{$chro}{$gName}{'reg'},$gName,$genes->{$chro}{$gName}{'foldChange'});
547 }
548
549 if (($type ne "intergenic")&&($type ne "NA")) {
550 unless (exists ($hashForOverlapingGenes{$type})) {
551 my %h;
552 $hashForOverlapingGenes{$type} = \%h;
553 }
554 $hashForOverlapingGenes{$type}->{$locDistTSS}=$gName;
555
556 #print OUT "$chro\t$fpos\t$lpos\t$pos\t$score\t$locDistTSS\t$type\t$genes->{$chro}{$gName}{'reg'}\t$gName\n" ;
557 $type = "NA";
558 #unless ($gName =~ m/$TSS/) {
559 # print "$gName\t$TSS\n";
560 #}
561 }
562
563 }
564 if ($type eq "intergenic") {
565 print OUT "$chro\t$fpos\t$lpos\t$pos\t$score\t$distTSS\t$type\t$typeIntra\t$reg\t$foldChange\t$distTE\t$geneName\n" ;
566 #print "$chro\t$fpos\t$lpos\t$pos\t$score\t$distTSS\t$type\t$reg\t$geneName\n" ;
567 push(@array2return,$type);
568
569 } else {
570 my $selectedType;
571 if (exists ($hashForOverlapingGenes{"promoter"})) {
572 $selectedType = "promoter";
573 } elsif (exists ($hashForOverlapingGenes{"immediateDownstream"})) {
574 $selectedType = "immediateDownstream";
575 } elsif (exists ($hashForOverlapingGenes{"intragenic"})) {
576 $selectedType = "intragenic";
577 } elsif (exists ($hashForOverlapingGenes{"enhancer"})) {
578 $selectedType = "enhancer";
579 } elsif (exists ($hashForOverlapingGenes{"5kbDownstream"})) {
580 $selectedType = "5kbDownstream";
581 }
582 my $bestGene = printBest($hashForOverlapingGenes{$selectedType},$chro,$fpos,$lpos,$pos,$score,$genes,$selectedType); #print "$type\n";
583 push(@array2return,$selectedType);
584 my %otherGenes;
585 for my $type ("promoter","immediateDownstream","intragenic","enhancer","5kbDownstream") { #"firstIntron","exon","intron",
586 for my $locDistTSS (sort {$a<=>$b} keys %{$hashForOverlapingGenes{$type}}) {
587 my $otherGene = $hashForOverlapingGenes{$type}->{$locDistTSS};
588 next if ($genes->{$chro}{$bestGene}{'name'} eq $genes->{$chro}{$otherGene}{'name'});
589 next if (exists ($otherGenes{$genes->{$chro}{$otherGene}{'name'}}));
590 if (overlapGenes($otherGene,$bestGene,$chro,$genes)) {
591
592 if (($type eq "intragenic")||($type eq "immediateDownstream")) {
593 $typeIntra = &getTypeIntra($genes->{$chro}{$otherGene},$pos);
594 $typehashIntra{$typeIntra}++;
595 }else {
596 $typeIntra = "NA"; }
597 print OUT "$chro\t$fpos\t$lpos\t$pos\t$score\t$locDistTSS\t$type\t$typeIntra\t$genes->{$chro}{$otherGene}{'reg'}\t$genes->{$chro}{$otherGene}{'foldChange'}\t",($pos-$genes->{$chro}{$otherGene}{'TE'})*$genes->{$chro}{$otherGene}{'strand'},"\t$otherGene\n" ;
598 push(@array2return,$type);
599
600 #print "$chro\t$fpos\t$lpos\t$pos\t$score\t$locDistTSS\t$type\t$genes->{$chro}{$otherGene}{'reg'}\t$otherGene\n" ;
601 #print $type,"\n";
602 $otherGenes{$genes->{$chro}{$otherGene}{'name'}} = 1;
603 }
604 }
605 }
606
607 }
608 return @array2return;
609 }
610
611 sub findAllClosestGeneOneLocation {
612 my ($fpos,$lpos,$score,$chro,$pos,$genes) = @_;
613 my ($distTSS,$type,$reg,$geneName,$foldChange) = ($BIGNUMBER,"intergenic",0,0,0);
614 my $locDistTSS = 0;
615 my $locDistTE = 0;
616 my %hashForOverlapingGenes;
617 my @array2return;
618 my $typeIntra = "NA";
619 for my $gName (keys %{$genes->{$chro}}) {
620 my $TSS = $genes->{$chro}{$gName}{'TSS'};
621 my $strand = $genes->{$chro}{$gName}{'strand'};
622 my $TE = $genes->{$chro}{$gName}{'TE'};
623
624 $locDistTE = ($pos-$TE)*$strand;
625 $locDistTSS = ($pos-$TSS)*$strand;
626 #print "$pos-$genes->{$chro}{$gName}{'TSS'})*$genes->{$chro}{$gName}{'strand'} = $locDistTSS\n";
627 #my $locDistTE = ($pos-$genes->{$chro}{$gName}{'TE'})*$genes->{$chro}{$gName}{'strand'};
628
629 #print "$gName $maxDistance $pos-$genes->{$chro}{$gName}{'TSS'} $genes->{$chro}{$gName}{'strand'}\n";
630 #print "$locDistTSS $enhLeft $enhRight\n";
631
632 if (($locDistTSS>= $enhLeft)&&($locDistTSS<$enhRight)) {
633 $type = "enhancer";
634 } elsif (($locDistTSS>= $enhRight)&&($locDistTSS<=0)) {
635 $type = "promoter";
636 } elsif (($locDistTSS> 0)&&($locDistTSS<=$immediateDownstream)&&($locDistTE<=0)) {
637 $type = "immediateDownstream";
638 } elsif (($locDistTSS >= $immediateDownstream)&&($locDistTE<=0)) {
639 $type = "intragenic";
640 } elsif (($locDistTE >= 0)&&($locDistTE<=$downStreamDist)) {
641 $type = "5kbDownstream";
642 } elsif (abs($locDistTSS)<abs($distTSS)) {
643 $distTSS = $locDistTSS;
644 ($reg,$geneName,$foldChange) = ($genes->{$chro}{$gName}{'reg'},$gName,$genes->{$chro}{$gName}{'foldChange'});
645 }
646
647 if (($type ne "intergenic")&&($type ne "NA")) {
648 unless (exists ($hashForOverlapingGenes{$type})) {
649 my %h;
650 $hashForOverlapingGenes{$type} = \%h;
651 $hashForOverlapingGenes{$type}->{$gName}=$locDistTSS;
652
653 if (($type eq "intragenic")||($type eq "immediateDownstream")) {
654 $typeIntra = &getTypeIntra($genes->{$chro}{$gName},$pos);
655 $typehashIntra{$typeIntra}++;
656 }
657 print OUT "$chro\t$fpos\t$lpos\t$pos\t$score\t$locDistTSS\t$type\t$typeIntra\t$genes->{$chro}{$gName}{'reg'}\t$genes->{$chro}{$gName}{'fluo'}\t$genes->{$chro}{$gName}{'foldChange'}\t$genes{$chro}->{$gName}{'GCisland'}\t",($pos-$genes->{$chro}{$gName}{'TE'})*$genes->{$chro}{$gName}{'strand'},"\t$gName\n" ;
658 push(@array2return,$type);
659 }
660 $typeIntra = "NA";
661 $type = "NA";
662 #unless ($gName =~ m/$TSS/) {
663 # print "$gName\t$TSS\n";
664 #}
665 }
666
667 }
668 if ($type eq "intergenic") {
669 print OUT "$chro\t$fpos\t$lpos\t$pos\t$score\t$locDistTSS\t$type\t$typeIntra\t$genes->{$chro}{$geneName}{'reg'}\t$genes->{$chro}{$geneName}{'fluo'}\t$genes->{$chro}{$geneName}{'foldChange'}\t$genes{$chro}->{$geneName}{'GCisland'}\t",($pos-$genes->{$chro}{$geneName}{'TE'})*$genes->{$chro}{$geneName}{'strand'},"\t$geneName\n" ;
670
671 #print "$chro\t$fpos\t$lpos\t$pos\t$score\t$distTSS\t$type\t$reg\t$geneName\n" ;
672 push(@array2return,$type);
673
674 }
675 return @array2return;
676 }
677
678 sub findAllClosestGene {
679 my ($fpos,$lpos,$score,$chro,$pos,$genes) = @_;
680 my ($distTSS,$type,$reg,$geneName,$foldChange) = ($BIGNUMBER,"intergenic",0,0,0);
681 my $locDistTSS = 0;
682 my $locDistTE = 0;
683 my %hashForOverlapingGenes;
684 my @array2return;
685 my $typeIntra = "NA";
686 for my $gName (keys %{$genes->{$chro}}) {
687 my $TSS = $genes->{$chro}{$gName}{'TSS'};
688 my $strand = $genes->{$chro}{$gName}{'strand'};
689 my $TE = $genes->{$chro}{$gName}{'TE'};
690
691 $locDistTE = ($pos-$TE)*$strand;
692 $locDistTSS = ($pos-$TSS)*$strand;
693 #print "$pos-$genes->{$chro}{$gName}{'TSS'})*$genes->{$chro}{$gName}{'strand'} = $locDistTSS\n";
694 #my $locDistTE = ($pos-$genes->{$chro}{$gName}{'TE'})*$genes->{$chro}{$gName}{'strand'};
695
696 #print "$gName $maxDistance $pos-$genes->{$chro}{$gName}{'TSS'} $genes->{$chro}{$gName}{'strand'}\n";
697 #print "$locDistTSS $enhLeft $enhRight\n";
698
699 if (($locDistTSS>= $enhLeft)&&($locDistTSS<$enhRight)) {
700 $type = "enhancer";
701 } elsif (($locDistTSS>= $enhRight)&&($locDistTSS<=0)) {
702 $type = "promoter";
703 } elsif (($locDistTSS> 0)&&($locDistTSS<=$immediateDownstream)&&($locDistTE<=0)) {
704 $type = "immediateDownstream";
705 } elsif (($locDistTSS >= $immediateDownstream)&&($locDistTE<=0)) {
706 $type = "intragenic";
707 } elsif (($locDistTE >= 0)&&($locDistTE<=$downStreamDist)) {
708 $type = "5kbDownstream";
709 } elsif (abs($locDistTSS)<abs($distTSS)) {
710 $distTSS = $locDistTSS;
711 ($reg,$geneName,$foldChange) = ($genes->{$chro}{$gName}{'reg'},$gName,$genes->{$chro}{$gName}{'foldChange'});
712 }
713
714 if (($type ne "intergenic")&&($type ne "NA")) {
715 unless (exists ($hashForOverlapingGenes{$type})) {
716 my %h;
717 $hashForOverlapingGenes{$type} = \%h;
718 }
719 #$hashForOverlapingGenes{$type}->{$gName}=$locDistTSS;
720
721 $typeIntra = "NA";
722 if (($type eq "intragenic")||($type eq "immediateDownstream")) {
723 $typeIntra = &getTypeIntra($genes->{$chro}{$gName},$pos);
724 $typehashIntra{$typeIntra}++;
725 }
726 print OUT "$chro\t$fpos\t$lpos\t$pos\t$score\t$locDistTSS\t$type\t$typeIntra\t$genes->{$chro}{$gName}{'reg'}\t$genes->{$chro}{$gName}{'fluo'}\t$genes->{$chro}{$gName}{'foldChange'}\t$genes{$chro}->{$gName}{'GCisland'}\t",($pos-$genes->{$chro}{$gName}{'TE'})*$genes->{$chro}{$gName}{'strand'},"\t$gName\n" ;
727 push(@array2return,$type);
728
729 $type = "NA";
730 #unless ($gName =~ m/$TSS/) {
731 # print "$gName\t$TSS\n";
732 #}
733 }
734
735 }
736 if ($type eq "intergenic") {
737 print OUT "$chro\t$fpos\t$lpos\t$pos\t$score\t$locDistTSS\t$type\t$typeIntra\t$genes->{$chro}{$geneName}{'reg'}\t$genes->{$chro}{$geneName}{'fluo'}\t$genes->{$chro}{$geneName}{'foldChange'}\t$genes{$chro}->{$geneName}{'GCisland'}\t",($pos-$genes->{$chro}{$geneName}{'TE'})*$genes->{$chro}{$geneName}{'strand'},"\t$geneName\n" ;
738
739 #print "$chro\t$fpos\t$lpos\t$pos\t$score\t$distTSS\t$type\t$reg\t$geneName\n" ;
740 push(@array2return,$type);
741
742 }
743 return @array2return;
744 }
745
746 sub getTypeIntra {
747
748 my ($geneEntry, $pos) = @_;
749 my $type;
750
751 if (($pos >= $geneEntry->{'firstIntronStart'})&&($pos <=$geneEntry->{'firstIntronEnd'})) {
752 return "f_intron";
753 }
754 if (($pos >= $geneEntry->{'firstExonStart'})&&($pos <=$geneEntry->{'firstExonEnd'})) {
755 return "f_exon";
756 }
757 $type = getIntronExon ($pos, $geneEntry->{'exonCount'},$geneEntry->{'exonStarts'},$geneEntry->{'exonEnds'},$geneEntry->{'strand'});
758 return $type;
759 }
760
761 sub overlapGenes {
762 my ($otherGene,$bestGene,$chro,$genes)= @_;
763 my $a1 = $genes->{$chro}{$otherGene}{'left'};
764 my $a2 = $genes->{$chro}{$bestGene}{'left'};
765 my $e1 = $genes->{$chro}{$otherGene}{'right'};
766 my $e2 = $genes->{$chro}{$bestGene}{'right'};
767 if (($a1 >= $a2)&&($a1 <= $e2)) {
768 return 1;
769 }
770 if (($a2 >= $a1)&&($a2 <= $e1)) {
771 return 1;
772 }
773 return 0;
774 }
775
776 sub printBest {
777 my ($hash,$chro,$fpos,$lpos,$pos,$score,$genes,$type) = @_;
778 for my $locDistTSS (sort {$a<=>$b} keys %{$hash}) {
779 my $gName = $hash->{$locDistTSS};
780 my $typeIntra = "NA";
781 if (($type eq "intragenic")||($type eq "immediateDownstream")) {
782 $typeIntra = &getTypeIntra($genes->{$chro}{$gName},$pos);
783 $typehashIntra{$typeIntra}++;
784 }
785 print OUT "$chro\t$fpos\t$lpos\t$pos\t$score\t$locDistTSS\t$type\t$typeIntra\t$genes->{$chro}{$gName}{'reg'}\t$genes->{$chro}{$gName}{'foldChange'}\t",($pos-$genes->{$chro}{$gName}{'TE'})*$genes->{$chro}{$gName}{'strand'},"\t$gName\n" ;
786 return $gName;
787 }
788 }
789
790 #sub findAllClosestGene {
791 # my ($fpos,$lpos,$score,$chro,$pos,$genes) = @_;
792 # my ($distTSS,$type,$reg,$geneName,$foldChange) = ($BIGNUMBER,"intergenic",0,0,0);
793 # my $locDistTSS = 0;
794 # my $locDistTE = 0;
795 # my @array2return;
796 # for my $gName (keys %{$genes->{$chro}}) {
797 # my $TSS = $genes->{$chro}{$gName}{'TSS'};
798 # my $strand = $genes->{$chro}{$gName}{'strand'};
799 # my $TE = $genes->{$chro}{$gName}{'TE'};
800 # $locDistTSS = ($pos-$TSS)*$strand;
801 # $locDistTE = ($pos-$TE)*$strand;
802 # #print "$pos-$genes->{$chro}{$gName}{'TSS'})*$genes->{$chro}{$gName}{'strand'} = $locDistTSS\n";
803 # #my $locDistTE = ($pos-$genes->{$chro}{$gName}{'TE'})*$genes->{$chro}{$gName}{'strand'};
804 #
805 # #print "$gName $maxDistance $pos-$genes->{$chro}{$gName}{'TSS'} $genes->{$chro}{$gName}{'strand'}\n";
806 # #print "$locDistTSS $enhLeft $enhRight\n";
807 #
808 # if (($locDistTSS>= $enhLeft)&&($locDistTSS<$enhRight)) {
809 # $type = "enhancer";
810 # } elsif (($locDistTSS>= $enhRight)&&($locDistTSS<=0)) {
811 # $type = "promoter";
812 # } elsif (($locDistTSS> 0)&&($locDistTSS<=$immediateDownstream)) {
813 # $type = "immediateDownstream";
814 # } elsif (($pos >= $genes{$chro}->{$gName}{'firstIntronStart'})&&($pos <= $genes{$chro}->{$gName}{'firstIntronEnd'})) {
815 # $type = "firstIntron";
816 # } elsif (($locDistTSS> $immediateDownstream)&&($locDistTSS<=$genes{$chro}->{$gName}{'length'})) {
817 # #$type = "intragenic";
818 # $type = &getIntronExon ($pos, $genes{$chro}->{$gName}{'exonCount'},$genes{$chro}->{$gName}{'exonStarts'},$genes{$chro}->{$gName}{'exonEnds'},$genes{$chro}->{$gName}{'strand'});
819 # } elsif (($locDistTE >= 0)&&($locDistTE<=$downStreamDist)) {
820 # $type = "5kbDownstream";
821 # } elsif (abs($locDistTSS)<abs($distTSS)) {
822 # $distTSS = $locDistTSS;
823 # ($reg,$geneName,$foldChange) = ($genes->{$chro}{$gName}{'reg'},$gName,$genes->{$chro}{$gName}{'foldChange'});
824 # }
825 # if (($type ne "intergenic")&&($type ne "NA")) {
826 # print OUT "$chro\t$fpos\t$lpos\t$pos\t$score\t$locDistTSS\t$type\t$genes->{$chro}{$gName}{'reg'}\t$genes->{$chro}{$gName}{'foldChange'}\t$gName\n" ;
827 # push(@array2return,$type);
828 # $type = "NA";
829 # #unless ($gName =~ m/$TSS/) {
830 # # print "$gName\t$TSS\n";
831 # #}
832 # }
833 #
834 # }
835 # if ($type eq "intergenic") {
836 # print OUT "$chro\t$fpos\t$lpos\t$pos\t$score\t$distTSS\t$type\t$reg\t$foldChange\t$geneName\n" ;
837 # push(@array2return,$type);
838 # #print "$chro\t$fpos\t$lpos\t$pos\t$score\t$distTSS\t$type\t$reg\t$geneName\n" ;
839 #
840 # }
841 # return @array2return;
842 #}
843
844 sub getFirstIntron {
845 my ($exonCount,$exonStarts,$exonEnds,$strand) = @_;
846 my ($left,$right);
847 if ($exonCount == 1) {
848 return (0,0);
849 }
850 if ($strand == 1) {
851 $left = (split ",", $exonEnds)[0]+$jonctionSize;
852 $right = (split (",", $exonStarts))[1]-$jonctionSize;
853 } else {
854 $left = (split (",", $exonEnds))[$exonCount-2]+$jonctionSize;
855 $right = (split (",", $exonStarts))[$exonCount-1]-$jonctionSize;
856 }
857 ($left,$right);
858 }
859
860 sub getFirstExon {
861 my ($exonCount,$exonStarts,$exonEnds,$strand) = @_;
862 my ($left,$right);
863 if ($exonCount == 1) {
864 return (0,0);
865 }
866 if ($strand == 1) {
867 $left = (split ",", $exonStarts)[0];
868 $right = (split (",", $exonEnds))[0]-$jonctionSize;
869 } else {
870 $left = (split (",", $exonStarts))[$exonCount-1]+$jonctionSize;
871 $right = (split (",", $exonEnds))[$exonCount-1];
872 }
873 ($left,$right);
874 }
875
876 sub getIntronExon {
877 my ($pos,$exonCount,$exonStarts,$exonEnds,$strand) = @_;
878 my (@left,@right);
879 @left = (split ",", $exonStarts);
880 @right = (split (",", $exonEnds));
881
882 for (my $i = 0; $i<$exonCount;$i++) {
883 #print "$left[$i] <= $pos ? $pos <= $right[$i]\n";
884 if (($left[$i]+$jonctionSize < $pos) && ($pos < $right[$i]-$jonctionSize)) {
885 #print "URA!\n";
886 return "exon";
887 } elsif (($i+1<$exonCount)&&($right[$i]+$jonctionSize < $pos) && ($pos < $left[$i+1]-$jonctionSize)) {
888 return "intron";
889 }
890 }
891 return "jonction";
892 }
893
894 sub min {
895 return $_[0] if ($_[0]<$_[1]);
896 $_[1];
897 }
898 sub med {
899 my @arr = @_;
900 my $med = 0;
901 @arr = sort {$a <=> $b} @arr;
902 if ((scalar(@arr)/2) =~ m/[\.\,]5/) {
903 return $arr[floor(scalar(@arr)/2)];
904 } else {
905 return ($arr[scalar(@arr)/2]+$arr[scalar(@arr)/2-1])/2;
906 }
907 $med;
908 }
909
910 sub checkIfGC {
911 my ($TSS,$strand,$dist,$GCislandsChr)=@_;
912 my $ifGC = 0;
913 my $leftProm=$TSS-$dist;
914 my $rightProm = $TSS;
915 if ($strand== -1) {
916 my $leftProm=$TSS;
917 my $rightProm = $TSS+$dist;
918 }
919 for my $leftGC (keys %{$GCislandsChr}) {
920 my $rightGC = $GCislandsChr->{$leftGC};
921 if ($leftGC>=$leftProm&&$leftGC<=$rightProm || $rightGC>=$leftProm&&$rightGC<=$rightProm) {
922 return "GC-island";
923 }
924 }
925 return $ifGC ;
926 }