# HG changeset patch # User iuc # Date 1722449235 0 # Node ID 1f380e8a6aecc49839bc0017852da17764e94c26 # Parent 69a12ab93cddc29d0ced2c2aadd4763eb78e482f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit c21958f44b81d740191999fb6015d5ae69538ee0 diff -r 69a12ab93cdd -r 1f380e8a6aec macros.xml --- a/macros.xml Wed Sep 22 21:07:11 2021 +0000 +++ b/macros.xml Wed Jul 31 18:07:15 2024 +0000 @@ -1,12 +1,17 @@ - 1.7.1 - 19.01 - + 1.9.6 + 4 + 21.09 - scanpy - loompy - leidenalg + scanpy + loompy + leidenalg + louvain + pandas + matplotlib + seaborn + magic-impute @@ -15,9 +20,15 @@ scanpy + + + + + 10.1186/s13059-017-1382-0 + 10.1093/gigascience/giaa102 @@ -56,7 +67,7 @@ @@ -66,12 +77,12 @@ - + advanced_common['show_log'] @@ -414,6 +425,7 @@ + @@ -421,6 +433,9 @@ + + + @@ -457,15 +472,17 @@ @@ -491,15 +508,15 @@ var_group_positions=$var_group_positions, var_group_labels=$var_group_labels, #end if -#if $method.var_group_rotation - var_group_rotation=$method.var_group_rotation, -#end if -#if $method.figsize.test == 'yes' - figsize=($method.figsize.width, $method.figsize.height), -#end if -#if $method.layer != '' - layer='$method.layer', -#end if + #if str($method.var_group_rotation) != '' + var_group_rotation=$method.var_group_rotation, + #end if + #if $method.figsize.test == 'yes' + figsize=($method.figsize.width, $method.figsize.height), + #end if + #if $method.layer + layer='$method.layer', + #end if ]]> @@ -652,9 +669,8 @@ - + - @@ -692,17 +708,19 @@ @@ -742,13 +760,7 @@ horizontal - + @@ -778,7 +790,9 @@ orient='$method.seaborn_violinplot.orient', #end if linewidth=$method.seaborn_violinplot.linewidth, + #if $method.seaborn_violinplot.color color='$method.seaborn_violinplot.color', + #end if saturation=$method.seaborn_violinplot.saturation ]]> @@ -787,7 +801,7 @@ - - + - - + @@ -931,7 +943,7 @@ - + @@ -942,7 +954,9 @@ #if str($method.edges.edges) == 'True' edges=True, edges_width=$method.edges.edges_width, + #if $method.edges.edges_color edges_color='$method.edges.edges_color', + #end if #else edges=False, #end if @@ -952,7 +966,7 @@ - + @@ -979,10 +993,10 @@ @CMD_param_legend_fontsize@ legend_fontweight='$method.plot.legend_fontweight', @CMD_param_size@ - #if str($method.plot.color_map) != 'None' + #if $method.plot.color_map color_map='$method.plot.color_map', #end if - #if str($method.plot.palette) != '' + #if $method.plot.palette palette='$method.plot.palette', #end if frameon=$method.plot.frameon, @@ -1052,18 +1066,18 @@ - + - + @@ -1123,7 +1139,9 @@ @@ -1208,7 +1226,7 @@
- + @@ -1220,15 +1238,19 @@
- + @@ -1255,7 +1277,9 @@ swap_axes=$method.swap_axes, @CMD_conditional_stripplot@ scale='$method.violin_plot.scale', + #if $method.row_palette row_palette='$method.row_palette', + #end if #if str($method.standard_scale) != 'None' standard_scale='$method.standard_scale', #end if diff -r 69a12ab93cdd -r 1f380e8a6aec plot.xml --- a/plot.xml Wed Sep 22 21:07:11 2021 +0000 +++ b/plot.xml Wed Jul 31 18:07:15 2024 +0000 @@ -1,9 +1,9 @@ - + with scanpy - macros.xml + @@ -21,23 +21,44 @@ #if $method.method == 'pl.scatter' +#if $method.type.type == 'xy': +x_field='$method.type.x' +y_field='$method.type.y' + +#if $method.type.log: +if x_field in adata.obs or x_field in adata.var: + if x_field in adata.obs: + adata.obs[f"log_{x_field}"] = np.log10(adata.obs[x_field]) + elif x_field in adata.var: + adata.var[f"log_{x_field}"] = np.log10(adata.var[x_field]) + x_field=f"log_{x_field}" + +if y_field in adata.obs or y_field in adata.var: + if y_field in adata.obs: + adata.obs[f"log_{y_field}"] = np.log10(adata.obs[y_field]) + elif y_field in adata.var: + adata.var[f"log_{y_field}"] = np.log10(adata.var[y_field]) + y_field=f"log_{y_field}" +#end if +#end if + sc.pl.scatter( @CMD_param_plot_inputs@ - #if $method.type.type == 'xy' - x='$method.type.x', - y='$method.type.y', - #if str($method.type.color) != '' + #if $method.type.type == 'xy': + x=x_field, + y=y_field, + #if $method.type.color color='$method.type.color', - #end if - #if str('$method.type.layers.use_layers') == 'true' + #end if + #if str('$method.type.layers.use_layers') == 'true' layers=('$method.type.layers.layer_x', '$method.type.layers.layer_y', '$method.type.layers.layer_color'), - #end if + #end if #else basis='$method.type.basis', - #if str($method.type.color) != '' - #set $color = ([x.strip() for x in str($method.type.color).split(',')]) + #if $method.type.color + #set $color = ([x.strip() for x in str($method.type.color).split(',')]) color=$color, - #end if + #end if #end if use_raw=$method.use_raw, sort_order=$method.sort_order, @@ -45,10 +66,10 @@ @CMD_params_pl_components@ projection='$method.plot.projection', legend_loc='$method.plot.legend_loc', - #if $method.plot.palette != 'default' + #if $method.plot.palette palette=['$method.plot.palette'], #end if - #if $method.plot.color_map != 'None' + #if $method.plot.color_map color_map='$method.plot.color_map', #end if @CMD_param_legend_fontsize@ @@ -60,7 +81,7 @@ #else if $method.method == 'pl.heatmap' sc.pl.heatmap( @CMD_param_plot_inputs@ - #if str($method.gene_symbols) != '' + #if $method.gene_symbols gene_symbols='$method.gene_symbols', #end if @CMD_params_inputs@ @@ -70,7 +91,7 @@ #else if $method.method == 'pl.dotplot' sc.pl.dotplot( @CMD_param_plot_inputs@ - #if str($method.gene_symbols) != '' + #if $method.gene_symbols gene_symbols='$method.gene_symbols', #end if @CMD_params_inputs@ @@ -102,7 +123,7 @@ #if $method.xlabel xlabel='$method.xlabel', #end if - #if $method.rotation + #if str($method.rotation) != '' rotation=$method.rotation, #end if @CMD_params_seaborn_violinplot@) @@ -110,7 +131,7 @@ #else if $method.method == 'pl.stacked_violin' sc.pl.stacked_violin( @CMD_param_plot_inputs@ - #if str($method.gene_symbols) != '' + #if $method.gene_symbols gene_symbols='$method.gene_symbols', #end if @CMD_params_inputs@ @@ -120,7 +141,7 @@ #else if $method.method == 'pl.matrixplot' sc.pl.matrixplot( @CMD_param_plot_inputs@ - #if str($method.gene_symbols) != '' + #if $method.gene_symbols gene_symbols='$method.gene_symbols', #end if @CMD_params_inputs@ @@ -135,7 +156,7 @@ adata=adata, #end if show=False, - #if str($method.obs_keys) != '' + #if $method.obs_keys obs_keys='$method.obs_keys', #end if use_raw=$method.use_raw, @@ -154,13 +175,13 @@ sc.pl.highest_expr_genes( @CMD_param_plot_inputs@ n_top=$method.n_top, - #if str($method.gene_symbols) != '' + #if $method.gene_symbols gene_symbols='$method.gene_symbols', #end if - #if str($method.setseaborn_boxplot.color) != '' + #if $method.setseaborn_boxplot.color color='$method.setseaborn_boxplot.color', #end if - #if str($method.setseaborn_boxplot.palette) != 'None' + #if $method.setseaborn_boxplot.palette palette='$method.setseaborn_boxplot.palette', #end if saturation=$method.setseaborn_boxplot.saturation) @@ -248,7 +269,7 @@ sc.pl.draw_graph( @CMD_param_plot_inputs@ @CMD_param_color@ - #if str($method.gene_symbols) != '' + #if $method.gene_symbols gene_symbols='$method.gene_symbols', #end if use_raw=$method.use_raw, @@ -265,10 +286,33 @@ @CMD_pl_attribute_section@ @CMD_params_matplotlib_pyplot_scatter@) +#else if $method.method == 'pl.embedding_density' +sc.pl.embedding_density( + @CMD_param_plot_inputs@ + basis='$method.basis', + #if $method.key + key='$method.key', + #end if + #if $method.groupby + groupby='$method.groupby', + #end if + #if $method.group + #set $group = ([x.strip() for x in str($method.group).split(',')]) + group=$group, + #end if + #if $method.color_map + color_map='$method.color_map', + #end if + bg_dotsize=$method.bg_dotsize, + fg_dotsize=$method.fg_dotsize, + ncols=$method.ncols, + wspace=$method.wspace, + hspace=$method.hspace) + #else if $method.method == 'pl.dpt_groups_pseudotime' sc.pl.dpt_groups_pseudotime( @CMD_param_plot_inputs@ - #if str($method.color_map) != '' + #if $method.color_map color_map='$method.color_map' #end if ) @@ -277,7 +321,7 @@ sc.pl.dpt_timeseries( @CMD_param_plot_inputs@ #if $method.heatmap.as_heatmap == "True" - #if str($method.heatmap.color_map) != '' + #if $method.heatmap.color_map color_map='$method.heatmap.color_map', #end if #end if @@ -301,15 +345,15 @@ #set $keys=([x.strip() for x in str($method.keys).split(',')]) keys=$keys, use_raw=$method.use_raw, - #if str($method.annotations) != '' + #if $method.annotations #set $annotations=([x.strip() for x in str($method.annotations).split(',')]) annotations=$annotations, #end if - #if str($method.color_map) != '' + #if $method.color_map color_map='$method.color_map', #end if n_avg=$method.n_avg, - #if str($method.annotations) != '' + #if $method.annotations groups_key='$method.groups_key', #end if as_heatmap=$method.as_heatmap, @@ -323,7 +367,7 @@ @CMD_param_plot_inputs@ @CMD_params_groups@ n_genes=$method.n_genes, - #if str($method.gene_symbols) != '' + #if $method.gene_symbols gene_symbols='$method.gene_symbols', #end if fontsize=$method.fontsize, @@ -340,7 +384,7 @@ #set $gene_names = ([x.strip() for x in str($method.genes.gene_names).split(',')]) gene_names=$gene_names, #end if - #if str($method.gene_symbols) != '' + #if $method.gene_symbols gene_symbols='$method.gene_symbols', #end if use_raw=$method.use_raw, @@ -358,6 +402,9 @@ sc.pl.rank_genes_groups_dotplot( @CMD_param_plot_inputs@ @CMD_pl_rank_genes_groups_ext@ + #if $method.gene_symbols + gene_symbols='$method.gene_symbols', + #end if @CMD_params_plots@ @CMD_pl_dotplot@) @@ -366,6 +413,9 @@ @CMD_param_plot_inputs@ @CMD_pl_rank_genes_groups_ext@ @CMD_params_plots@ + #if $method.gene_symbols + gene_symbols='$method.gene_symbols', + #end if @CMD_pl_heatmap@) #else if $method.method == 'pl.rank_genes_groups_matrixplot' @@ -373,6 +423,9 @@ @CMD_param_plot_inputs@ @CMD_pl_rank_genes_groups_ext@ @CMD_params_plots@ + #if $method.gene_symbols + gene_symbols='$method.gene_symbols', + #end if @CMD_pl_matrixplot@) #else if $method.method == 'pl.rank_genes_groups_stacked_violin' @@ -380,6 +433,9 @@ @CMD_param_plot_inputs@ @CMD_pl_rank_genes_groups_ext@ @CMD_params_plots@ + #if $method.gene_symbols + gene_symbols='$method.gene_symbols', + #end if @CMD_pl_stacked_violin@) #end if @@ -397,7 +453,6 @@ - @@ -409,15 +464,14 @@ + - @@ -436,6 +490,7 @@ + @@ -666,6 +721,27 @@ + + + + + + + + + + + + + + + + + + + + @@ -730,7 +806,7 @@ - + @@ -745,45 +821,49 @@ + + + + - + format == 'png' and method['method'] != 'pl.rank_genes_groups_violin' - + format == 'png' and method['method'] == 'pl.rank_genes_groups_violin' - - format == 'pdf' and method['method'] != pl.rank_genes_groups_violin + + format == 'pdf' and method['method'] != 'pl.rank_genes_groups_violin' - + format == 'pdf' and method['method'] == 'pl.rank_genes_groups_violin' - + format == 'svg' and method['method'] != pl.rank_genes_groups_violin - + format == 'svg' and method['method'] == 'pl.rank_genes_groups_violin' @@ -792,8 +872,8 @@ - - + + @@ -837,8 +917,8 @@ - - + + @@ -870,8 +950,8 @@ - - + + @@ -885,8 +965,8 @@ - - + + @@ -933,8 +1013,8 @@ - - + + @@ -983,8 +1063,8 @@ - - + + @@ -1036,14 +1116,14 @@ - - + + - - + + @@ -1080,8 +1160,8 @@ - - + + @@ -1137,8 +1217,8 @@ - - + + @@ -1197,8 +1277,8 @@ - - + + @@ -1232,8 +1312,8 @@ - - + + @@ -1270,14 +1350,14 @@ - + - - + + @@ -1316,8 +1396,8 @@ - - + + @@ -1348,8 +1428,8 @@ - - + + @@ -1375,8 +1455,8 @@ - - + + @@ -1397,8 +1477,8 @@ - - + + @@ -1452,14 +1532,14 @@ - - + + - - + + @@ -1477,8 +1557,8 @@ - - + + @@ -1498,8 +1578,8 @@ - - + + @@ -1541,8 +1621,8 @@ - - + + @@ -1590,14 +1670,14 @@ - - + + - - + + @@ -1650,14 +1730,14 @@ - - + + - - + + @@ -1701,14 +1781,14 @@ - - + + - - + + @@ -1757,28 +1837,54 @@ - - + + - + + + + + + + +
+ +
+ + + + + + + + +
+ + - - - - +
+ +
+ + + + + + -
!--> - - + + + @@ -1800,15 +1906,14 @@ - - - + @@ -1823,19 +1928,40 @@ -
!--> - - + + + + + + + + + + + + + + + + + + + + +
+ - - + --> + + + @@ -1855,8 +1981,8 @@ - @@ -1899,13 +2025,13 @@
- --> - - + + + @@ -1927,18 +2053,19 @@ - - + + - - + + + @@ -1969,12 +2096,13 @@ - - + + + @@ -1998,18 +2126,19 @@ - + - - + + + @@ -2057,6 +2186,53 @@ + + + + + + + + + + + + + + + + + +
+ + + + + + + + +
+
+
+ +
+ + + + + + + + + + + + + + + +
`__ +`__ Generic: Heatmap of the expression values of set of genes (`pl.heatmap`) ======================================================================== @@ -2078,7 +2254,7 @@ specified in `num_categories`. More details on the `scanpy documentation -`__ +`__ Generic: Makes a dot plot of the expression values (`pl.dotplot`) ================================================================= @@ -2095,7 +2271,7 @@ expressing the gene can be visualized for each cluster. More details on the `scanpy documentation -`__ +`__ Generic: Violin plot (`pl.violin`) ================================== @@ -2103,7 +2279,7 @@ Wraps `seaborn.violinplot` for `anndata.AnnData`. More details on the `scanpy documentation -`__ +`__ Generic: Stacked violin plots (`pl.stacked_violin`) =================================================== @@ -2112,7 +2288,7 @@ stacked on top of each other. Useful to visualize gene expression per cluster. More details on the `scanpy documentation -`__ +`__ Generic: Heatmap of the mean expression values per cluster (`pl.matrixplot`) ============================================================================ @@ -2123,7 +2299,7 @@ category. More details on the `scanpy documentation -`__ +`__ Generic: Hierarchically-clustered heatmap (`pl.clustermap`) =========================================================== @@ -2141,7 +2317,7 @@ Column indices, use: clustergrid.dendrogram_col.reordered_ind More details on the `scanpy documentation -`__ +`__ Preprocessing: Plot the fraction of counts assigned to each gene over all cells (`pl.highest_expr_genes`) ========================================================================================================= @@ -2153,7 +2329,7 @@ This plot is similar to the `scater` package function `plotHighestExprs(type= "highest-expression")` More details on the `scanpy documentation -`__ +`__ Preprocessing: Plot dispersions versus means for genes (`pl.highly_variable_genes`) =================================================================================== @@ -2161,25 +2337,25 @@ It produces Supp. Fig. 5c of Zheng et al. (2017) and MeanVarPlot() of Seurat. More details on the `scanpy documentation -`__ +`__ PCA: Scatter plot in PCA coordinates (`pl.pca`) =============================================== More details on the `scanpy documentation -`__ +`__ PCA: Rank genes according to contributions to PCs (`pl.pca_loadings`) ===================================================================== More details on the `scanpy documentation -`__ +`__ PCA: Plot the variance ratio (`pl.pca_variance_ratio`) ====================================================== More details on the `scanpy documentation -`__ +`__ PCA: Plot PCA results (`pl.pca_overview`) ========================================= @@ -2188,37 +2364,37 @@ if you want to change the default settings. More details on the `scanpy documentation -`__ +`__ Embedding: Scatter plot in tSNE basis (`pl.tsne`) ================================================= More details on the `scanpy documentation -`__ +`__ Embeddings: Scatter plot in UMAP basis (`pl.umap`) ================================================== More details on the `scanpy documentation -`__ +`__ Embeddings: Scatter plot in Diffusion Map basis (`pl.diffmap`) ============================================================== More details on the `scanpy documentation -`__ +`__ Branching trajectories and pseudotime, clustering: Plot groups and pseudotime (`pl.dpt_groups_pseudotime`) =========================================================================================================== More details on the `scanpy documentation -`__ +`__ Branching trajectories and pseudotime, clustering: Heatmap of pseudotime series (`pl.dpt_timeseries`) ===================================================================================================== More details on the `scanpy documentation -`__ +`__ Branching trajectories and pseudotime, clustering: Plot the abstracted graph through thresholding low-connectivity edges (`pl.paga`) @@ -2231,56 +2407,56 @@ `maxiter` parameter by 1 if the layout is flipped. More details on the `scanpy documentation -`__ +`__ Branching trajectories and pseudotime, clustering: Scatter and PAGA graph side-by-side (`pl.paga_compare`) ========================================================================================================== More details on the `scanpy documentation -`__ +`__ Branching trajectories and pseudotime, clustering: Gene expression and annotation changes along paths (`pl.paga_path`) ====================================================================================================================== More details on the `scanpy documentation -`__ +`__ Marker genes: Plot ranking of genes using dotplot plot (`pl.rank_genes_groups`) =============================================================================== More details on the `scanpy documentation -`__ +`__ Marker genes: Plot ranking of genes as violin plot (`pl.rank_genes_groups_violin`) ================================================================================== More details on the `scanpy documentation -`__ +`__ Marker genes: Plot ranking of genes as dotplot plot (`pl.rank_genes_groups_dotplot`) ==================================================================================== More details on the `scanpy documentation -`__ +`__ Marker genes: Plot ranking of genes as heatmap plot (`pl.rank_genes_groups_heatmap`) ==================================================================================== More details on the `scanpy documentation -`__ +`__ Marker genes: Plot ranking of genes as matrixplot plot (`pl.rank_genes_groups_matrixplot`) ========================================================================================== More details on the `scanpy documentation -`__ +`__ Marker genes: Plot ranking of genes as stacked violin plot (`pl.rank_genes_groups_stacked_violin`) ================================================================================================== More details on the `scanpy documentation -`__ +`__ ]]>
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FTH1 HCK AIF1 SAT1 CTSS S100A11 MS4A7 TYROBP COTL1 STXBP2 RP11-290F20.3 S100A4 IFITM2 SPI1 DUSP1 SESN2 IFITM3 MPP1 GALE CORO1B RP11-390E23.6 VIMP RSBN1L-AS1 CHD4 CFP GSTP1 PFN1 FCGRT ADTRP ARHGDIB AMICA1 HLA-DRB5 CST3 GRN HLA-DPA1 SSR3 +CD19+ B TNFRSF13B CD79B SMARCB1 PNOC CCDC50 AL928768.3 BANK1 MS4A1 CD79A ISG20 IGLL5 TNFRSF17 KIAA0125 TPD52 PEBP1 FKBP11 CCDC132 SUB1 POU2AF1 MZB1 PTPRCAP UBE2J1 BLK SPIB DERL3 FAM63B MPHOSPH9 IGJ FCRLA XBP1 NCF1 SSR3 CD52 TSHZ2 PDLIM1 VIMP SSR4 S1PR4 SELL HMGA1 NUCB2 JUN CD27 ARHGDIB GYPC CALR ADTRP BTG1 EXOG RARRES3 +CD34+ PRSS57 C19orf77 SPINK2 RP11-620J15.3 SNHG7 CYTL1 EGFL7 NGFRAP1 SOX4 NFE2 EGR1 RP3-467N11.1 H1FX CDK6 SERPINB1 SPINT2 HMGA1 IL1B NUCB2 RPLP0 IGFBP7 RPLP1 ATXN7L3B RPS3 C1orf228 KIAA0125 RPL3 SYPL1 CD63 LDHB SEPT1 JUN FAM101B PRKCQ-AS1 MATK PEBP1 SELL ITM2A SSR3 SPON2 XBP1 UBE2J1 VIMP GYPC STK17A STMN1 VIM MZB1 HOPX CD99 +CD4+/CD25 T Reg IL32 SPOCK2 ACTG1 CD2 CD3D GPR171 ARHGDIB ACOX1 MAL SIT1 GIMAP4 AES CD52 SEPT1 TMSB10 LAT STMN1 LINC00402 CD27 TSHZ2 S1PR4 CD3E PFN1 CD99 AQP3 PTPRCAP CD3G LY9 LCK CD247 S100A4 CCR7 TTC39C CORO1B MPHOSPH9 FYB RPSA FLT3LG B2M GIMAP7 PRKCQ-AS1 SELL BTG1 CCDC132 GYPC DENND2D LDHB IL7R ITM2A RPLP0 +CD4+/CD45RA+/CD25- Naive T EAF2 GNG7 SSR4 CALR DERL3 MANF IGJ XBP1 ATXN7L3B SSR3 UBE2J1 CD79A MZB1 RP3-467N11.1 TNFRSF17 NCF1 CDK6 SUB1 POU2AF1 AL928768.3 FKBP11 VIMP GYPC JUN CD27 PEBP1 SMARCB1 FLT3LG RPLP1 RPLP0 CCDC50 ISG20 IGLL5 HCST GSTP1 GPX1 CD52 VIM PTPRCAP FCGRT CD74 B2M RPL3 CYTL1 SPINK2 PRSS57 C19orf77 RP11-620J15.3 FAM101B CCDC132 +CD4+/CD45RO+ Memory RNF138 NOSIP IFITM1 LCK RARRES3 ALOX5AP FAM63B RAB3IP GZMK CD3G SEPT1 LDHB SELL CD3D EXOG RPSA CD247 AES CD52 TMSB10 NUCB2 DENND2D RPL3 RPLP1 ACTG1 FYB GIMAP7 CORO1B LY9 CD7 PFN1 RPS3 GYPC CD2 ARHGDIB IL32 RPLP0 CD99 CD3E GIMAP4 HCST B2M LAT ISG20 ITM2A FKBP11 SERPINB1 STK17A CCR7 PTPRCAP +CD56+ NK CST7 SPON2 HOPX GNLY NKG7 CTSW KLRC2 CD7 MATK PCIF1 CLIC3 FGFBP2 SYPL1 GZMB C9orf142 PRF1 CD247 HCST GZMA GZMH STMN1 ALOX5AP CD63 CD99 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