# HG changeset patch # User iuc # Date 1510153315 18000 # Node ID 6090793a47e85cdfb89ca1d4abdd76a37e66fa8f # Parent 831dcb48efa17000ad4863877268e60dbda96c6c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/minimap2 commit 160b3085e5c9152fc8eaeb2e8afc12cdc1abe739 diff -r 831dcb48efa1 -r 6090793a47e8 minimap2.xml --- a/minimap2.xml Sat Nov 04 05:40:54 2017 -0400 +++ b/minimap2.xml Wed Nov 08 10:01:55 2017 -0500 @@ -1,8 +1,8 @@ - + A fast pairwise aligner for genomic and spliced nucleotide sequences - minimap2 + minimap2 samtools minimap2 --version @@ -429,14 +429,12 @@ recent samtools will throw an error and abort. Older samtools and other tools may create corrupted BAM. -To avoid this issue, you can add option ``-L`` at the minimap2 command -line. This option moves a long CIGAR to the ``CG`` tag and leaves a -fully clipped CIGAR at the SAM CIGAR column. Current tools that don’t -read CIGAR (e.g. merging and sorting) still work with such BAM records; -tools that read CIGAR will effectively ignore these records. I have pull -requests to the SAM spec, htslib, htsjdk, bedtools2, Rsamtools and -igv.js. If they are accepted, future versions of these tools will -seamlessly recognize long-cigar records generated by option ``-L``. +To avoid this issue, you can add option ``-L`` at the minimap2 command line. +This option moves a long CIGAR to the ``CG`` tag and leaves a fully clipped +CIGAR at the SAM CIGAR column. Current tools that don’t read CIGAR +(e.g. merging and sorting) still work with such BAM records; tools that read +CIGAR will effectively ignore these records. It has been decided that future +tools will seamlessly recognize long-cigar records generated by option `-L`. **TD;DR**: if you work with ultra-long reads and use tools that only process BAM files, please add option ``-L``. diff -r 831dcb48efa1 -r 6090793a47e8 test-data/minimap2-test1-fasta.bam Binary file test-data/minimap2-test1-fasta.bam has changed diff -r 831dcb48efa1 -r 6090793a47e8 test-data/minimap2-test1.bam Binary file test-data/minimap2-test1.bam has changed diff -r 831dcb48efa1 -r 6090793a47e8 test-data/minimap2-test2.bam Binary file test-data/minimap2-test2.bam has changed