# HG changeset patch # User iuc # Date 1521726196 14400 # Node ID 27e369f4407a828849e03b401dc73dc846663ea5 # Parent 9f27508afc8c1dd285ed492e13b21a6f753b3f0d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/macs2 commit 09ef7ee96fadeef22273029ea23d1e140ce96737 diff -r 9f27508afc8c -r 27e369f4407a macs2_bdgdiff.xml --- a/macs2_bdgdiff.xml Tue Mar 06 07:25:01 2018 -0500 +++ b/macs2_bdgdiff.xml Thu Mar 22 09:43:16 2018 -0400 @@ -1,4 +1,4 @@ - + Differential peak detection based on paired four bedgraph files macs2_macros.xml @@ -23,7 +23,7 @@ -o c1.bed c2.bed both.bed && awk '!/^track name/' "c1.bed" > '${ output_cond1 }' && - awk '!/^track name/' "c2.bed" > '${ output_cond1 }' && + awk '!/^track name/' "c2.bed" > '${ output_cond2 }' && awk '!/^track name/' "both.bed" > '${ output_both }' ]]> @@ -51,7 +51,6 @@ - @@ -61,11 +60,13 @@ - + + + diff -r 9f27508afc8c -r 27e369f4407a macs2_callpeak.xml --- a/macs2_callpeak.xml Tue Mar 06 07:25:01 2018 -0500 +++ b/macs2_callpeak.xml Thu Mar 22 09:43:16 2018 -0400 @@ -1,4 +1,4 @@ - + Call peaks from alignment results macs2_macros.xml @@ -243,34 +243,34 @@ - + outputs and 'peaks_tabular' in outputs - + (( advanced_options['broad_options']['broad_options_selector'] == "broad" )) - + (( advanced_options['broad_options']['broad_options_selector'] == "broad" )) - + (( advanced_options['broad_options']['broad_options_selector'] == "nobroad" )) - + outputs and 'summits' in outputs - + (( outputs and 'pdf' in outputs and @@ -279,13 +279,13 @@ )) - + outputs and 'bdg' in outputs - + outputs and 'bdg' in outputs - + outputs and 'html' in outputs @@ -349,8 +349,6 @@ **callpeak** is the main function of the MACS2_ package. MACS identifies enriched binding sites in ChIP-seq experiments. It captures the influence of genome complexity to evaluate the significance of enriched ChIP regions, and improves the spatial resolution of binding sites through combining the information of both sequencing tag position and orientation. -.. _MACS2: https://github.com/taoliu/MACS - ----- **Inputs** @@ -372,6 +370,8 @@ ce: 9e7 dm: 1.2e8 +Or see the **deepTools** website for updated information on calculating `Effective Genome Size`_. + ----- **Outputs** @@ -587,6 +587,8 @@ If MACS2 fails, it is usually because it cannot build the model for peaks. You may want to extend **mfold** range by increasing the upper bound or play with **Build model** options. For more information, see the MACS2_ website. +.. _MACS2: https://github.com/taoliu/MACS +.. _`Effective Genome Size`: http://deeptools.readthedocs.io/en/latest/content/feature/effectiveGenomeSize.html .. _`UCSC website here`: https://genome.ucsc.edu/goldenPath/help/bedgraph.html @citation@ diff -r 9f27508afc8c -r 27e369f4407a test-data/bdgdiff_output_both.bed diff -r 9f27508afc8c -r 27e369f4407a test-data/bdgdiff_output_c1.bed diff -r 9f27508afc8c -r 27e369f4407a test-data/bdgdiff_output_c2.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/bdgdiff_output_c2.bed Thu Mar 22 09:43:16 2018 -0400 @@ -0,0 +1,1 @@ +chrV 6194 6538 c2.bed1 7.25269