Mercurial > repos > iuc > ena_upload
comparison ena_upload.xml @ 0:b7356ece7ceb draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ena_upload commit 57b434bcf493554d060a99b65e66f274d5c00e0a"
| author | iuc |
|---|---|
| date | Sat, 28 Nov 2020 09:45:14 +0000 |
| parents | |
| children | bd624f0547fc |
comparison
equal
deleted
inserted
replaced
| -1:000000000000 | 0:b7356ece7ceb |
|---|---|
| 1 <tool id="ena_upload" name="ENA Upload tool" version="0.3" profile="20.01" license="MIT"> | |
| 2 <macros> | |
| 3 <token name="@VERSION@">0.2.4</token> | |
| 4 <import>samples_macros.xml</import> | |
| 5 </macros> | |
| 6 <requirements> | |
| 7 <requirement type="package" version="@VERSION@">ena-upload-cli</requirement> | |
| 8 <requirement type="package" version="1.2.0">xlrd</requirement> | |
| 9 </requirements> | |
| 10 <command detect_errors="exit_code"><![CDATA[ | |
| 11 mkdir ./submission_files; | |
| 12 | |
| 13 #set working_dir = os.getcwd() | |
| 14 #set $dry_run_option = "False" | |
| 15 #set viral_submission = "False" | |
| 16 #if $action_options.input_format_conditional.input_format == "build_tables": | |
| 17 python '$__tool_directory__/extract_tables.py' --action $action_options.action --out_dir ./submission_files --studies $studies_json; | |
| 18 #set $studies_table_path = './submission_files/studies.tsv' | |
| 19 #set $samples_table_path = './submission_files/samples.tsv' | |
| 20 #set $experiments_table_path = './submission_files/experiments.tsv' | |
| 21 #set $runs_table_path = './submission_files/runs.tsv' | |
| 22 #end if | |
| 23 | |
| 24 #if $action_options.input_format_conditional.input_format == "excel_tables": | |
| 25 python '$__tool_directory__/process_xlsx.py' | |
| 26 #if $action_options.input_format_conditional.viral_submission == "true": | |
| 27 --vir | |
| 28 #end if | |
| 29 --action '$action_options.action' --form '$action_options.input_format_conditional.xlsx_file' --out_dir ./submission_files ; | |
| 30 #set $studies_table_path = './submission_files/studies.tsv' | |
| 31 #set $samples_table_path = './submission_files/samples.tsv' | |
| 32 #set $experiments_table_path = './submission_files/experiments.tsv' | |
| 33 #set $runs_table_path = './submission_files/runs.tsv' | |
| 34 #if $action_options.input_format_conditional.dry_run == "true": | |
| 35 #set $dry_run_option = "True" | |
| 36 #end if | |
| 37 #end if | |
| 38 | |
| 39 #if $action_options.input_format_conditional.input_format != "user_generated_tables": | |
| 40 cp $studies_table_path $studies_table_out; | |
| 41 cp $samples_table_path $samples_table_out; | |
| 42 cp $experiments_table_path $experiments_table_out; | |
| 43 cp $runs_table_path $runs_table_out; | |
| 44 #if $action_options.input_format_conditional.dry_run == "true": | |
| 45 #set $dry_run_option = "True" | |
| 46 #end if | |
| 47 #end if | |
| 48 | |
| 49 | |
| 50 #if $dry_run_option == "False" and $action_options.test_submit == "False": | |
| 51 webin_id=`grep 'username' $credentials`; | |
| 52 if [ "\$webin_id" = "" ]; then | |
| 53 ## No credentials in user defined preferences | |
| 54 ## Fallback to global defined credentials (if exist) | |
| 55 #import os | |
| 56 #if os.path.isfile(os.environ.get('GALAXY_ENA_SECRETS', '')): | |
| 57 credentials_path=\${ENA_SECRETS}; | |
| 58 webin_id=`grep 'username' \$GALAXY_ENA_SECRETS`; | |
| 59 if [ "\$webin_id" = "" ]; then | |
| 60 echo "No global credentials defined. Check your GALAXY_ENA_SECRETS file or set your credentials via: User -> Preferences -> Manage Information"; | |
| 61 exit 1; | |
| 62 fi; | |
| 63 #else: | |
| 64 echo "No ENA credentials defined. Set your credentials via: User -> Preferences -> Manage Information"; | |
| 65 exit 1; | |
| 66 #end if | |
| 67 else | |
| 68 credentials_path='$credentials'; | |
| 69 fi; | |
| 70 #end if | |
| 71 | |
| 72 #if $action_options.test_submit == "True": | |
| 73 credentials_path='test_fake_path'; | |
| 74 #end if | |
| 75 | |
| 76 ## create the list of files to upload and make the symlinks | |
| 77 #import re | |
| 78 #set $files_to_upload = list() | |
| 79 #if $action_options.input_format_conditional.input_format == "build_tables": | |
| 80 #for $study in $action_options.input_format_conditional.conditional_viral_metadata.rep_study: | |
| 81 #for $sample in $study.rep_sample: | |
| 82 #for $experiment in $sample.rep_experiment: | |
| 83 #for $run in $experiment.rep_runs: | |
| 84 #for $file in $run.upload_files: | |
| 85 #set $safename_reads_file = re.sub('[^\w\-_\.]', '_', $file.element_identifier) | |
| 86 ln -s '$file' $safename_reads_file && | |
| 87 $files_to_upload.append(str($safename_reads_file)) | |
| 88 #end for | |
| 89 #end for | |
| 90 #end for | |
| 91 #end for | |
| 92 #end for | |
| 93 #else: | |
| 94 #for $file in $action_options.input_format_conditional.data: | |
| 95 #set $safename_reads_file = re.sub('[^\w\-_\.]', '_', $file.element_identifier) | |
| 96 ln -s '$file' $safename_reads_file && | |
| 97 $files_to_upload.append(str($safename_reads_file)) | |
| 98 #end for | |
| 99 #end if | |
| 100 | |
| 101 | |
| 102 #if $dry_run_option == "False": | |
| 103 ena-upload-cli | |
| 104 --tool 'ena-upload-cli v@VERSION@ @ Galaxy' | |
| 105 --action '$action_options.action' | |
| 106 --center '$action_options.center' | |
| 107 --secret \${credentials_path} | |
| 108 --data | |
| 109 #for $dataset in $files_to_upload: | |
| 110 '$dataset' | |
| 111 #end for | |
| 112 #if $action_options.input_format_conditional.input_format == "user_generated_tables": | |
| 113 --experiment '$action_options.input_format_conditional.experiments_users_table' | |
| 114 --study '$action_options.input_format_conditional.studies_users_table' | |
| 115 --run '$action_options.input_format_conditional.runs_users_table' | |
| 116 --sample '$action_options.input_format_conditional.samples_users_table' | |
| 117 #if "$action_options.input_format_conditional.viral_submission" == "true": | |
| 118 --vir | |
| 119 #end if | |
| 120 #else: | |
| 121 --experiment '$experiments_table_path' | |
| 122 --study '$studies_table_path' | |
| 123 --run '$runs_table_path' | |
| 124 --sample '$samples_table_path' | |
| 125 #if $action_options.input_format_conditional.input_format == "build_tables": | |
| 126 #if $action_options.input_format_conditional.conditional_viral_metadata.viral_sample == "true": | |
| 127 --vir | |
| 128 #end if | |
| 129 #else: | |
| 130 #if $action_options.input_format_conditional.viral_submission == "true": | |
| 131 --vir | |
| 132 #end if | |
| 133 #end if | |
| 134 #end if | |
| 135 | |
| 136 #if $action_options.submit_dev == "true": | |
| 137 -d | |
| 138 #end if | |
| 139 > '$output' | |
| 140 #else: | |
| 141 echo "" | |
| 142 exit 0; | |
| 143 #end if | |
| 144 | |
| 145 ]]></command> | |
| 146 <configfiles> | |
| 147 <configfile name="credentials"><![CDATA[ | |
| 148 #set $webin_id = $__user__.extra_preferences.get('ena_webin_account|webin_id', "").strip() | |
| 149 #set $password = $__user__.extra_preferences.get('ena_webin_account|password', "").strip() | |
| 150 #if $webin_id != "": | |
| 151 username: "$webin_id" | |
| 152 password: "$password" | |
| 153 #end if | |
| 154 ]]></configfile> | |
| 155 <configfile name="studies_json"> | |
| 156 #import json | |
| 157 #import re | |
| 158 #if $action_options.input_format_conditional.input_format == "build_tables": | |
| 159 #set $files_to_upload = list() | |
| 160 #set $studies = list() | |
| 161 #for $study in $action_options.input_format_conditional.conditional_viral_metadata.rep_study: | |
| 162 #set samples = list() | |
| 163 #for $sample in $study.rep_sample: | |
| 164 #set experiments = list() | |
| 165 #for $experiment in $sample.rep_experiment: | |
| 166 #set runs = list() | |
| 167 #for $run in $experiment.rep_runs: | |
| 168 #set run_files = list() | |
| 169 #for $file in $run.upload_files: | |
| 170 #set $safename_reads_file = re.sub('[^\w\-_\.]', '_', $file.element_identifier) | |
| 171 $run_files.append(str($safename_reads_file)) | |
| 172 #end for | |
| 173 $runs.append($run_files) | |
| 174 #end for | |
| 175 | |
| 176 $experiments.append({'title':str($experiment.experiment_title),'experiment_design':str($experiment.experiment_design),'library_strategy':str($experiment.library_strategy),'library_source':str($experiment.library_source),'library_selection':str($experiment.library_selection),'library_layout':str($experiment.library_layout),'insert_size':str($experiment.insert_size),'library_construction_protocol':str($experiment.library_construction_protocol),'platform':str($experiment.platform),'instrument_model':str($experiment.instrument_model),'runs':$runs}) | |
| 177 #end for | |
| 178 #if $action_options.input_format_conditional.conditional_viral_metadata.viral_sample == "true": | |
| 179 | |
| 180 $samples.append({'title':str($sample.sample_title),'description':str($sample.sample_description),'tax_name':str($sample.scientific_name),'tax_id':str($sample.tax_id),'collection_date':str($sample.collection_date),'geo_location':str($sample.geo_location_country),'host_common_name':str($sample.host_common_name),'host_subject_id':str($sample.host_subject_id),'host_health_state':str($sample.host_health_state),'host_sex':str($sample.host_sex),'host_scientific_name':str($sample.host_scientific_name),'collector_name':str($sample.collector_name),'collecting_institution':str($sample.collecting_institution),'isolate':str($sample.isolate),'experiments':$experiments}) | |
| 181 #else: | |
| 182 | |
| 183 $samples.append({'title':str($sample.sample_title),'description':str($sample.sample_description),'tax_name':str($sample.scientific_name),'tax_id':str($sample.tax_id),'experiments':$experiments}) | |
| 184 #end if | |
| 185 #end for | |
| 186 $studies.append({'title':str($study.study_title),'type':str($study.study_type),'abstract':str($study.study_abstract),'pubmed_id':str($study.study_pubmed_id),'samples':$samples}) | |
| 187 #end for | |
| 188 #echo $json.dumps($studies) | |
| 189 #end if | |
| 190 </configfile> | |
| 191 </configfiles> | |
| 192 <inputs> | |
| 193 <conditional name="action_options"> | |
| 194 <param name="action" type="select" label="Action to execute"> | |
| 195 <option value="add" selected="True">Add new data</option> | |
| 196 <option value="modify">Modify metadata</option> | |
| 197 </param> | |
| 198 <when value="add"> | |
| 199 <param name="submit_dev" type="boolean" label="Submit to test ENA server?" help="By selecting yes the reads will be submitted " /> | |
| 200 <param name="test_submit" type="hidden" value="False" /> | |
| 201 <expand macro="table_inputs_macro" /> | |
| 202 </when> | |
| 203 <when value="modify"> | |
| 204 <expand macro="table_inputs_macro" /> | |
| 205 </when> | |
| 206 </conditional> | |
| 207 </inputs> | |
| 208 <outputs> | |
| 209 <data name="output" format="data" label="${tool.name} on ${on_string}: Upload summary"/> | |
| 210 <data name="studies_table_out" format="tabular" label="Studies table"> | |
| 211 <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> | |
| 212 </data> | |
| 213 <data name="samples_table_out" format="tabular" label="Samples table"> | |
| 214 <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> | |
| 215 </data> | |
| 216 <data name="experiments_table_out" format="tabular" label="Experiments table"> | |
| 217 <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> | |
| 218 </data> | |
| 219 <data name="runs_table_out" format="tabular" label="Runs table"> | |
| 220 <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> | |
| 221 </data> | |
| 222 </outputs> | |
| 223 <tests> | |
| 224 <!--Test excel input of VIRAL samples --> | |
| 225 <test> | |
| 226 <conditional name="action_options"> | |
| 227 <param name="action" value="add"/> | |
| 228 <param name="submit_dev" value="False"/> | |
| 229 <conditional name="input_format_conditional"> | |
| 230 <param name="input_format" value="excel_tables"/> | |
| 231 <param name="viral_submission" value="True"/> | |
| 232 <param name="dry_run" value="True"/> | |
| 233 <param name="xlsx_file" value="metadata_test_viral.xlsx"/> | |
| 234 <param name="data" value="sample.fq"/> | |
| 235 </conditional> | |
| 236 </conditional> | |
| 237 <param name="center" value="Some research center"/> | |
| 238 <output name="experiments_table_out"> | |
| 239 <assert_contents> | |
| 240 <has_n_lines n="5"/> | |
| 241 <has_n_columns n="17"/> | |
| 242 <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_alias\tsample_alias\tdesign_description\tlibrary_name\tlibrary_strategy\tlibrary_source\tlibrary_selection\tlibrary_layout\tinsert_size\tlibrary_construction_protocol\tplatform\tinstrument_model\tsubmission_date" /> | |
| 243 <has_line_matching expression="e_(.*)_026\tadd\taccession_ena\tNanopore sequencing\tSARS-CoV-2_genomes_01\ts_(.*)"/> | |
| 244 </assert_contents> | |
| 245 </output> | |
| 246 <output name="studies_table_out"> | |
| 247 <assert_contents> | |
| 248 <has_n_lines n="2"/> | |
| 249 <has_n_columns n="8"/> | |
| 250 <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_type\tstudy_abstract\tpubmed_id\tsubmission_date"/> | |
| 251 <has_line_matching expression="SARS-CoV-2_genomes_01\tadd\tENA_accession\tWhole-genome sequencing of SARS-CoV-2 from Covid-19 patients\tWhole Genome Sequencing\tWhole-genome sequences of SARS-CoV-2 from oro-pharyngeal swabs obtained from Covid-19 patients(.*)"/> | |
| 252 </assert_contents> | |
| 253 </output> | |
| 254 <output name="samples_table_out"> | |
| 255 <assert_contents> | |
| 256 <has_n_lines n="5"/> | |
| 257 <has_n_columns n="18"/> | |
| 258 <has_line_matching expression="alias\tstatus\taccession\ttitle\tscientific_name\ttaxon_id\tsample_description\tcollection_date\tgeographic_location\thost_common_name\thost_subject_id\thost_health_state\thost_sex\thost_scientific_name\tcollector_name\tcollecting_institution\tisolate\tsubmission_date"/> | |
| 259 </assert_contents> | |
| 260 </output> | |
| 261 <output name="runs_table_out"> | |
| 262 <assert_contents> | |
| 263 <has_n_lines n="5"/> | |
| 264 <has_n_columns n="8"/> | |
| 265 <has_line_matching expression="alias\tstatus\taccession\texperiment_alias\tfile_name\tfile_format\tfile_checksum\tsubmission_date"/> | |
| 266 <has_line_matching expression="r_(.*)_026\tadd\tena_run_accession\te_(.*)_026\tC026_exp5_clean.fastq.gz\tfastq\tfile_checksum\tsubmission_date_ENA"/> | |
| 267 </assert_contents> | |
| 268 </output> | |
| 269 </test> | |
| 270 <!--Test excel input of NON-VIRAL samples--> | |
| 271 <test> | |
| 272 <conditional name="action_options"> | |
| 273 <param name="action" value="add"/> | |
| 274 <param name="submit_dev" value="False"/> | |
| 275 <conditional name="input_format_conditional"> | |
| 276 <param name="input_format" value="excel_tables"/> | |
| 277 <param name="viral_submission" value="False"/> | |
| 278 <param name="dry_run" value="True"/> | |
| 279 <param name="xlsx_file" value="metadata_test_nonviral.xlsx"/> | |
| 280 <param name="data" value="sample.fq"/> | |
| 281 </conditional> | |
| 282 </conditional> | |
| 283 <param name="center" value="Some research center"/> | |
| 284 <output name="experiments_table_out"> | |
| 285 <assert_contents> | |
| 286 <has_n_lines n="5"/> | |
| 287 <has_n_columns n="17"/> | |
| 288 <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_alias\tsample_alias\tdesign_description\tlibrary_name\tlibrary_strategy\tlibrary_source\tlibrary_selection\tlibrary_layout\tinsert_size\tlibrary_construction_protocol\tplatform\tinstrument_model\tsubmission_date" /> | |
| 289 </assert_contents> | |
| 290 </output> | |
| 291 <output name="studies_table_out"> | |
| 292 <assert_contents> | |
| 293 <has_n_lines n="2"/> | |
| 294 <has_n_columns n="8"/> | |
| 295 <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_type\tstudy_abstract\tpubmed_id\tsubmission_date"/> | |
| 296 </assert_contents> | |
| 297 </output> | |
| 298 <output name="samples_table_out"> | |
| 299 <assert_contents> | |
| 300 <has_n_lines n="5"/> | |
| 301 <has_n_columns n="8"/> | |
| 302 <has_line_matching expression="alias\tstatus\taccession\ttitle\tscientific_name\ttaxon_id\tsample_description\tsubmission_date"/> | |
| 303 </assert_contents> | |
| 304 </output> | |
| 305 <output name="runs_table_out"> | |
| 306 <assert_contents> | |
| 307 <has_n_lines n="5"/> | |
| 308 <has_n_columns n="8"/> | |
| 309 <has_line_matching expression="alias\tstatus\taccession\texperiment_alias\tfile_name\tfile_format\tfile_checksum\tsubmission_date"/> | |
| 310 <has_line_matching expression="r_(.*)_026\tadd\tena_run_accession\te_(.*)_026\tC026_exp5_clean.fastq.gz\tfastq\tfile_checksum\tsubmission_date_ENA"/> | |
| 311 </assert_contents> | |
| 312 </output> | |
| 313 </test> | |
| 314 <!--Test build tables from user input fields NON-VIRAL samples--> | |
| 315 <test> | |
| 316 <conditional name="action_options"> | |
| 317 <param name="action" value="add"/> | |
| 318 <param name="submit_dev" value="False"/> | |
| 319 <conditional name="input_format_conditional"> | |
| 320 <param name="input_format" value="build_tables"/> | |
| 321 <param name="dry_run" value="True"/> | |
| 322 <conditional name="conditional_viral_metadata"> | |
| 323 <param name="viral_sample" value="False"/> | |
| 324 <repeat name="rep_study"> | |
| 325 <param name="study_title" value="Test study title"/> | |
| 326 <param name="study_abstract" value="Test study abstract"/> | |
| 327 <param name="study_type" value="Epigenetics"/> | |
| 328 <param name="study_pubmed_id" value="Test study pubmedID"/> | |
| 329 <repeat name="rep_sample"> | |
| 330 <param name="sample_title" value="Test Sample title"/> | |
| 331 <param name="sample_description" value="Test Sample description"/> | |
| 332 <param name="scientific_name" value="Test Sample scientific name"/> | |
| 333 <param name="tax_id" value="Test Sample tax_id"/> | |
| 334 <repeat name="rep_experiment"> | |
| 335 <param name="experiment_title" value="Test experiment title"/> | |
| 336 <param name="experiment_design" value="Test experiment design description"/> | |
| 337 <param name="library_strategy" value="CTS"/> | |
| 338 <param name="library_source" value="GENOMIC"/> | |
| 339 <param name="library_selection" value="PCR"/> | |
| 340 <param name="library_layout" value="SINGLE"/> | |
| 341 <param name="insert_size" value="150"/> | |
| 342 <param name="library_construction_protocol" value="Test library construction"/> | |
| 343 <param name="platform" value="ILLUMINA"/> | |
| 344 <param name="instrument_model" value="Illumina HiSeq 4000"/> | |
| 345 <repeat name="rep_runs"> | |
| 346 <param name="upload_files" value="1.fastqsanger.gz,sample.fq" ftype="fastqsanger"/> | |
| 347 </repeat> | |
| 348 </repeat> | |
| 349 </repeat> | |
| 350 </repeat> | |
| 351 </conditional> | |
| 352 </conditional> | |
| 353 </conditional> | |
| 354 <param name="center" value="Some research center"/> | |
| 355 <output name="experiments_table_out"> | |
| 356 <assert_contents> | |
| 357 <has_n_lines n="2"/> | |
| 358 <has_n_columns n="17"/> | |
| 359 </assert_contents> | |
| 360 </output> | |
| 361 <output name="studies_table_out"> | |
| 362 <assert_contents> | |
| 363 <has_n_lines n="2"/> | |
| 364 <has_n_columns n="8"/> | |
| 365 <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_type\tstudy_abstract\tpubmed_id\tsubmission_date"/> | |
| 366 </assert_contents> | |
| 367 </output> | |
| 368 <output name="samples_table_out"> | |
| 369 <assert_contents> | |
| 370 <has_n_lines n="2"/> | |
| 371 <has_n_columns n="8"/> | |
| 372 </assert_contents> | |
| 373 </output> | |
| 374 <output name="runs_table_out"> | |
| 375 <assert_contents> | |
| 376 <has_n_lines n="3"/> | |
| 377 <has_n_columns n="8"/> | |
| 378 <has_line_matching expression="alias\tstatus\taccession\texperiment_alias\tfile_name\tfile_format\tfile_checksum\tsubmission_date"/> | |
| 379 </assert_contents> | |
| 380 </output> | |
| 381 </test> | |
| 382 <!--Test RUN failing build tables from user input fields NON-VIRAL samples--> | |
| 383 <test expect_failure="true"> | |
| 384 <conditional name="action_options"> | |
| 385 <param name="action" value="add"/> | |
| 386 <param name="submit_dev" value="True"/> | |
| 387 <conditional name="input_format_conditional"> | |
| 388 <param name="input_format" value="build_tables"/> | |
| 389 <param name="dry_run" value="False"/> | |
| 390 <conditional name="conditional_viral_metadata"> | |
| 391 <param name="viral_sample" value="False"/> | |
| 392 <repeat name="rep_study"> | |
| 393 <param name="study_title" value="Test study title"/> | |
| 394 <param name="study_abstract" value="Test study abstract"/> | |
| 395 <param name="study_type" value="Epigenetics"/> | |
| 396 <param name="study_pubmed_id" value="Test study pubmedID"/> | |
| 397 <repeat name="rep_sample"> | |
| 398 <param name="sample_title" value="Test Sample title"/> | |
| 399 <param name="sample_description" value="Test Sample description"/> | |
| 400 <param name="scientific_name" value="Test Sample scientific name"/> | |
| 401 <param name="tax_id" value="Test Sample tax_id"/> | |
| 402 <repeat name="rep_experiment"> | |
| 403 <param name="experiment_title" value="Test experiment title"/> | |
| 404 <param name="experiment_design" value="Test experiment design description"/> | |
| 405 <param name="library_strategy" value="CTS"/> | |
| 406 <param name="library_source" value="GENOMIC"/> | |
| 407 <param name="library_selection" value="PCR"/> | |
| 408 <param name="library_layout" value="SINGLE"/> | |
| 409 <param name="insert_size" value="150"/> | |
| 410 <param name="library_construction_protocol" value="Test library construction"/> | |
| 411 <param name="platform" value="ILLUMINA"/> | |
| 412 <param name="instrument_model" value="Illumina HiSeq 4000"/> | |
| 413 <repeat name="rep_runs"> | |
| 414 <param name="upload_files" value="1.fastqsanger.gz,sample.fq" ftype="fastqsanger"/> | |
| 415 </repeat> | |
| 416 </repeat> | |
| 417 </repeat> | |
| 418 </repeat> | |
| 419 </conditional> | |
| 420 </conditional> | |
| 421 </conditional> | |
| 422 <param name="center" value="Some research center"/> | |
| 423 <assert_stdout> | |
| 424 <has_text_matching expression="No ENA credentials defined"/> | |
| 425 </assert_stdout> | |
| 426 </test> | |
| 427 <!--Test with submit_test to skip credentials checksRUN failing build tables from user input fields NON-VIRAL samples--> | |
| 428 <test expect_failure="true"> | |
| 429 <conditional name="action_options"> | |
| 430 <param name="action" value="add"/> | |
| 431 <param name="submit_dev" value="True"/> | |
| 432 <param name="test_submit" value="True"/> | |
| 433 <conditional name="input_format_conditional"> | |
| 434 <param name="input_format" value="build_tables"/> | |
| 435 <param name="dry_run" value="False"/> | |
| 436 <conditional name="conditional_viral_metadata"> | |
| 437 <param name="viral_sample" value="False"/> | |
| 438 <repeat name="rep_study"> | |
| 439 <param name="study_title" value="Test study title"/> | |
| 440 <param name="study_abstract" value="Test study abstract"/> | |
| 441 <param name="study_type" value="Epigenetics"/> | |
| 442 <param name="study_pubmed_id" value="Test study pubmedID"/> | |
| 443 <repeat name="rep_sample"> | |
| 444 <param name="sample_title" value="Test Sample title"/> | |
| 445 <param name="sample_description" value="Test Sample description"/> | |
| 446 <param name="scientific_name" value="Test Sample scientific name"/> | |
| 447 <param name="tax_id" value="Test Sample tax_id"/> | |
| 448 <repeat name="rep_experiment"> | |
| 449 <param name="experiment_title" value="Test experiment title"/> | |
| 450 <param name="experiment_design" value="Test experiment design description"/> | |
| 451 <param name="library_strategy" value="CTS"/> | |
| 452 <param name="library_source" value="GENOMIC"/> | |
| 453 <param name="library_selection" value="PCR"/> | |
| 454 <param name="library_layout" value="SINGLE"/> | |
| 455 <param name="insert_size" value="150"/> | |
| 456 <param name="library_construction_protocol" value="Test library construction"/> | |
| 457 <param name="platform" value="ILLUMINA"/> | |
| 458 <param name="instrument_model" value="Illumina HiSeq 4000"/> | |
| 459 <repeat name="rep_runs"> | |
| 460 <param name="upload_files" value="1.fastqsanger.gz,sample.fq" ftype="fastqsanger"/> | |
| 461 </repeat> | |
| 462 </repeat> | |
| 463 </repeat> | |
| 464 </repeat> | |
| 465 </conditional> | |
| 466 </conditional> | |
| 467 </conditional> | |
| 468 <param name="center" value="Some research center"/> | |
| 469 <assert_command> | |
| 470 <has_text_matching expression="ena-upload-cli"/> | |
| 471 <has_text_matching expression="--data '1.fastqsanger.gz' 'sample.fq'"/> | |
| 472 <has_text_matching expression="--action 'add' --center 'Some research center'"/> | |
| 473 </assert_command> | |
| 474 <assert_stderr> | |
| 475 <has_text_matching expression="ENA_upload: error: Oops, the file test_fake_path does not exist"/> | |
| 476 </assert_stderr> | |
| 477 </test> | |
| 478 <!--test viral submission - User input metadata--> | |
| 479 <test expect_failure="true"> | |
| 480 <conditional name="action_options"> | |
| 481 <param name="action" value="add"/> | |
| 482 <param name="submit_dev" value="False"/> | |
| 483 <param name="test_submit" value="True"/> | |
| 484 <conditional name="input_format_conditional"> | |
| 485 <param name="input_format" value="build_tables"/> | |
| 486 <param name="dry_run" value="False"/> | |
| 487 <conditional name="conditional_viral_metadata"> | |
| 488 <param name="viral_sample" value="True"/> | |
| 489 <repeat name="rep_study"> | |
| 490 <param name="study_title" value="Test study title"/> | |
| 491 <param name="study_abstract" value="Test study abstract"/> | |
| 492 <param name="study_type" value="Epigenetics"/> | |
| 493 <param name="study_pubmed_id" value="Test study pubmedID"/> | |
| 494 <repeat name="rep_sample"> | |
| 495 <param name="sample_title" value="Test Sample title"/> | |
| 496 <param name="sample_description" value="Test Sample description"/> | |
| 497 <param name="scientific_name" value="Test Sample scientific name"/> | |
| 498 <param name="tax_id" value="Test Sample tax_id"/> | |
| 499 <param name="collection_date" value="2020"/> | |
| 500 <param name="geo_location_country" value="Belgium"/> | |
| 501 <param name="host_common_name" value="Human"/> | |
| 502 <param name="host_subject_id" value="Patient_001"/> | |
| 503 <param name="host_health_state" value="healthy"/> | |
| 504 <param name="host_sex" value="female"/> | |
| 505 <param name="host_scientific_name" value="homo sapiens"/> | |
| 506 <param name="collector_name" value="John The Collector"/> | |
| 507 <param name="collecting_institution" value="Hospital 01"/> | |
| 508 <param name="isolate" value="sample_001"/> | |
| 509 <repeat name="rep_experiment"> | |
| 510 <param name="experiment_title" value="Test experiment title"/> | |
| 511 <param name="experiment_design" value="Test experiment design description"/> | |
| 512 <param name="library_strategy" value="CTS"/> | |
| 513 <param name="library_source" value="GENOMIC"/> | |
| 514 <param name="library_selection" value="PCR"/> | |
| 515 <param name="library_layout" value="SINGLE"/> | |
| 516 <param name="insert_size" value="150"/> | |
| 517 <param name="library_construction_protocol" value="Test library construction"/> | |
| 518 <param name="platform" value="ILLUMINA"/> | |
| 519 <param name="instrument_model" value="Illumina HiSeq 4000"/> | |
| 520 <repeat name="rep_runs"> | |
| 521 <param name="upload_files" value="1.fastqsanger.gz,sample.fq" ftype="fastqsanger"/> | |
| 522 </repeat> | |
| 523 </repeat> | |
| 524 </repeat> | |
| 525 </repeat> | |
| 526 </conditional> | |
| 527 </conditional> | |
| 528 </conditional> | |
| 529 <param name="center" value="Some research center"/> | |
| 530 <assert_command> | |
| 531 <has_text_matching expression="ena-upload-cli"/> | |
| 532 <has_text_matching expression="--data '1.fastqsanger.gz' 'sample.fq'"/> | |
| 533 <has_text_matching expression="--action 'add' --center 'Some research center'"/> | |
| 534 <has_text_matching expression="--vir"/> | |
| 535 </assert_command> | |
| 536 <assert_stderr> | |
| 537 <has_text_matching expression="ENA_upload: error: Oops, the file test_fake_path does not exist"/> | |
| 538 </assert_stderr> | |
| 539 </test> | |
| 540 </tests> | |
| 541 <help><![CDATA[ | |
| 542 This is a wrapper for the ENA upload tool in https://github.com/usegalaxy-eu/ena-upload-cli | |
| 543 The input metadata can be submitted following the tabular format of the templates in https://github.com/usegalaxy-eu/ena-upload-cli/tree/master/example_tables | |
| 544 It is also possible to submit an excel file by following the template in https://drive.google.com/file/d/1ncC22--tW2v-EI-te_r86sAZujIPAjlX/view?usp=sharing | |
| 545 For viral submissions a larger set of metadata is required, you can find the template in https://drive.google.com/file/d/1U4VdcczsIecIXxseV8svE1zO_CBUadog/view?usp=sharing | |
| 546 ]]></help> | |
| 547 <citations> | |
| 548 </citations> | |
| 549 </tool> |
