# HG changeset patch # User iuc # Date 1741548433 0 # Node ID 871214ac722eda01b72d781512c32efe272299de # Parent 356bec18c68da9a0e7b6f3b3670ce03ce4343e02 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/decontam commit 8eca6c026e7bd7ab2e471485cb93b8dee1cd2c07 diff -r 356bec18c68d -r 871214ac722e decontam.xml --- a/decontam.xml Mon Dec 09 16:04:03 2024 +0000 +++ b/decontam.xml Sun Mar 09 19:27:13 2025 +0000 @@ -1,4 +1,5 @@ + Identification and removal of contaminants macros.xml @@ -150,6 +151,18 @@ Currently, only the negative control sample method is implemented in this wrapper. +**Method** + +To identify contaminants decontam requires a defined set of “negative control” samples in + which sequencing was performed on blanks without any biological sample added. +Extraction controls are preferred, and in amplicon sequencing the negative controls + should also be carried through the PCR step, as each step in the workflow + has the potential to introduce new contaminants. + +Decontam then uses Fisher’s exact tests to detect weather a feature is a contaminant or not, +by comparing true samples to ngeative controls. For more details on the detection method see +the publication of decontam. + **Output** - If a phyloseq object is provided as input, the output will be a phyloseq