Mercurial > repos > iuc > data_manager_star_index_builder
comparison data_manager/macros.xml @ 9:5750f6d0d369 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/data_managers/data_manager_star_index_builder commit a8e319862d723654c372a6d71e5de76e052586a9"
author | iuc |
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date | Wed, 05 Aug 2020 13:36:20 +0000 |
parents | d2f4bfcf269a |
children | f639ff7dea45 |
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8:d2f4bfcf269a | 9:5750f6d0d369 |
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3 whenever you make changes to the following two version tokens! | 3 whenever you make changes to the following two version tokens! |
4 The data manager uses a symlink to this macro file to keep the STAR and | 4 The data manager uses a symlink to this macro file to keep the STAR and |
5 the index versions in sync, but you should manually adjust the +galaxy | 5 the index versions in sync, but you should manually adjust the +galaxy |
6 version number. --> | 6 version number. --> |
7 <!-- STAR version to be used --> | 7 <!-- STAR version to be used --> |
8 <token name="@VERSION@">2.7.2b</token> | 8 <token name="@VERSION@">2.7.5b</token> |
9 <!-- STAR index version compatible with this version of STAR | 9 <!-- STAR index version compatible with this version of STAR |
10 This is the STAR version that introduced the index structure expected | 10 This is the STAR version that introduced the index structure expected |
11 by the current version. | 11 by the current version. |
12 It can be found for any specific version of STAR with: | 12 It can be found for any specific version of STAR with: |
13 STAR -h | grep versionGenome | 13 STAR -h | grep versionGenome |
14 or by looking for the versionGenome parameter in source/parametersDefault | 14 or by looking for the versionGenome parameter in source/parametersDefault |
15 of STAR's source code --> | 15 of STAR's source code --> |
16 <token name="@IDX_VERSION@">2.7.1a</token> | 16 <token name="@IDX_VERSION@">2.7.4a</token> |
17 <token name="@IDX_DATA_TABLE@">rnastar_index2x_versioned</token> | |
17 | 18 |
18 <xml name="requirements"> | 19 <xml name="requirements"> |
19 <requirements> | 20 <requirements> |
20 <requirement type="package" version="@VERSION@">star</requirement> | 21 <requirement type="package" version="@VERSION@">star</requirement> |
21 <requirement type="package" version="1.9">samtools</requirement> | 22 <requirement type="package" version="1.9">samtools</requirement> |
25 | 26 |
26 <xml name="index_selection" token_with_gene_model="0"> | 27 <xml name="index_selection" token_with_gene_model="0"> |
27 <param argument="--genomeDir" name="genomeDir" type="select" | 28 <param argument="--genomeDir" name="genomeDir" type="select" |
28 label="Select reference genome" | 29 label="Select reference genome" |
29 help="If your genome of interest is not listed, contact the Galaxy team"> | 30 help="If your genome of interest is not listed, contact the Galaxy team"> |
30 <options from_data_table="rnastar_index2_versioned"> | 31 <options from_data_table="@IDX_DATA_TABLE@"> |
31 <filter type="static_value" column="4" value="@WITH_GENE_MODEL@" /> | 32 <filter type="static_value" column="4" value="@WITH_GENE_MODEL@" /> |
32 <filter type="static_value" column="5" value="@IDX_VERSION@" /> | 33 <filter type="static_value" column="5" value="@IDX_VERSION@" /> |
33 <filter type="sort_by" column="2" /> | 34 <filter type="sort_by" column="2" /> |
34 <validator type="no_options" message="No indexes are available for the selected input dataset" /> | 35 <validator type="no_options" message="No indexes are available for the selected input dataset" /> |
35 </options> | 36 </options> |
51 <xml name="dbKeyActions"> | 52 <xml name="dbKeyActions"> |
52 <actions> | 53 <actions> |
53 <conditional name="refGenomeSource.geneSource"> | 54 <conditional name="refGenomeSource.geneSource"> |
54 <when value="indexed"> | 55 <when value="indexed"> |
55 <action type="metadata" name="dbkey"> | 56 <action type="metadata" name="dbkey"> |
56 <option type="from_data_table" name="rnastar_index2_versioned" column="1" offset="0"> | 57 <option type="from_data_table" name="@IDX_DATA_TABLE@" column="1" offset="0"> |
57 <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/> | 58 <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/> |
58 <filter type="param_value" ref="refGenomeSource.GTFconditional.genomeDir" column="0"/> | 59 <filter type="param_value" ref="refGenomeSource.GTFconditional.genomeDir" column="0"/> |
59 </option> | 60 </option> |
60 </action> | 61 </action> |
61 </when> | 62 </when> |
74 STAR | 75 STAR |
75 --runMode genomeGenerate | 76 --runMode genomeGenerate |
76 --genomeDir 'tempstargenomedir' | 77 --genomeDir 'tempstargenomedir' |
77 --genomeFastaFiles '${refGenomeSource.genomeFastaFiles}' | 78 --genomeFastaFiles '${refGenomeSource.genomeFastaFiles}' |
78 ## Handle difference between indices with/without annotations | 79 ## Handle difference between indices with/without annotations |
79 #if str($refGenomeSource.GTFconditional.GTFselect) == 'with-gtf': | 80 #if 'GTFconditional' in $refGenomeSource: |
80 --sjdbOverhang '${refGenomeSource.GTFconditional.sjdbOverhang}' | 81 ## GTFconditional exists only in STAR, but not STARsolo |
81 --sjdbGTFfile '${refGenomeSource.GTFconditional.sjdbGTFfile}' | 82 #if str($refGenomeSource.GTFconditional.GTFselect) == 'with-gtf': |
82 #if str($refGenomeSource.GTFconditional.sjdbGTFfile.ext) == 'gff3': | 83 --sjdbOverhang '${refGenomeSource.GTFconditional.sjdbOverhang}' |
84 --sjdbGTFfile '${refGenomeSource.GTFconditional.sjdbGTFfile}' | |
85 #if str($refGenomeSource.GTFconditional.sjdbGTFfile.ext) == 'gff3': | |
86 --sjdbGTFtagExonParentTranscript Parent | |
87 #end if | |
88 #end if | |
89 #else: | |
90 ## ref genome selection is less complex for STARsolo cause | |
91 ## with-gtf is mandatory there | |
92 --sjdbOverhang '${refGenomeSource.sjdbOverhang}' | |
93 --sjdbGTFfile '${refGenomeSource.sjdbGTFfile}' | |
94 #if str($refGenomeSource.sjdbGTFfile.ext) == 'gff3': | |
83 --sjdbGTFtagExonParentTranscript Parent | 95 --sjdbGTFtagExonParentTranscript Parent |
84 #end if | 96 #end if |
85 #end if | 97 #end if |
86 #if str($refGenomeSource.genomeSAindexNbases): | 98 #if str($refGenomeSource.genomeSAindexNbases): |
87 --genomeSAindexNbases ${refGenomeSource.genomeSAindexNbases} | 99 --genomeSAindexNbases ${refGenomeSource.genomeSAindexNbases} |
108 #end if | 120 #end if |
109 #end if | 121 #end if |
110 #end if | 122 #end if |
111 #end if | 123 #end if |
112 ]]></token> | 124 ]]></token> |
125 <xml name="ref_selection"> | |
126 <param argument="--genomeFastaFiles" type="data" format="fasta" label="Select a reference genome" /> | |
127 <!-- Currently, this parameter is not exposed in the wrapper, | |
128 but used only in the tests to avoid excessive index sizes for | |
129 the tiny test genomes. --> | |
130 <param name="genomeSAindexNbases" type="hidden" value="" /> | |
131 </xml> | |
113 <xml name="stdio" > | 132 <xml name="stdio" > |
114 <stdio> | 133 <stdio> |
115 <regex match="FATAL error" source="both" level="fatal"/> | 134 <regex match="FATAL error" source="both" level="fatal"/> |
116 <regex match="EXITING: FATAL INPUT ERROR:" source="both" level="fatal"/> | 135 <regex match="EXITING: FATAL INPUT ERROR:" source="both" level="fatal"/> |
117 <regex match="EXITING: fatal error trying to allocate genome arrays, exception thrown: std::bad_alloc" source="both" level="fatal"/> | 136 <regex match="EXITING: fatal error trying to allocate genome arrays, exception thrown: std::bad_alloc" source="both" level="fatal"/> |
118 <regex match="\[sam_read1\] missing header\? Abort!" source="both" level="fatal"/> | 137 <regex match="\[sam_read1\] missing header\? Abort!" source="both" level="fatal"/> |
119 <yield /> | 138 <yield /> |
120 </stdio> | 139 </stdio> |
121 </xml> | 140 </xml> |
122 <xml name="refgenomehandling" > | |
123 <conditional name="refGenomeSource"> | |
124 <param name="geneSource" type="select" label="Custom or built-in reference genome" help="Built-ins were indexed using default options"> | |
125 <option value="indexed" selected="true">Use a built-in index</option> | |
126 <option value="history">Use reference genome from history and create temporary index</option> | |
127 </param> | |
128 <when value="indexed"> | |
129 <conditional name="GTFconditional"> | |
130 <param name="GTFselect" type="select" | |
131 label="Reference genome with or without an annotation" | |
132 help="Select the '... with builtin gene-model' option to select from the list of available indexes that were built with splice junction information. Select the '... without builtin gene-model' option to select from the list of available indexes without annotated splice junctions."> | |
133 <option value="without-gtf" selected='true'>use genome reference without builtin gene-model</option> | |
134 <option value="with-gtf">use genome reference with builtin gene-model</option> | |
135 </param> | |
136 <when value="with-gtf"> | |
137 <expand macro="index_selection" with_gene_model="1" /> | |
138 </when> | |
139 <when value="without-gtf"> | |
140 <expand macro="index_selection" with_gene_model="0" /> | |
141 <expand macro="@SJDBOPTIONS@" /> | |
142 </when> | |
143 </conditional> | |
144 </when> | |
145 <when value="history"> | |
146 <param argument="--genomeFastaFiles" type="data" format="fasta" label="Select a reference genome" /> | |
147 <!-- Currently, this parameter is not exposed in the wrapper, | |
148 but used only in the tests to avoid excessive index sizes for | |
149 the tiny test genomes. --> | |
150 <param name="genomeSAindexNbases" type="hidden" value="" /> | |
151 <conditional name="GTFconditional"> | |
152 <param name="GTFselect" type="select" | |
153 label="Build index with our without known splice junctions annotation" | |
154 help="To build an index with known splice junctions annotated, you will have to provide a GTF or GFF3 dataset that describes the gene models (the location of genes, transcripts and exons) known for the reference genome."> | |
155 <option value="without-gtf">build index without gene-model</option> | |
156 <option value="with-gtf">build index with gene-model</option> | |
157 </param> | |
158 <when value="with-gtf"> | |
159 <expand macro="@SJDBOPTIONS@" optional="false"/> | |
160 </when> | |
161 <when value="without-gtf" /> | |
162 </conditional> | |
163 </when> | |
164 </conditional> | |
165 </xml> | |
166 </macros> | 141 </macros> |