Mercurial > repos > greg > snpeff_v2_from_pablo
diff snpEff_2_1a/scripts/qqplot.pl @ 0:f8eaa3f8194b default tip
Uploaded snpEff_v2_1a_core.tgz from Pablo Cingolani
author | greg |
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date | Fri, 20 Apr 2012 14:47:09 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/snpEff_2_1a/scripts/qqplot.pl Fri Apr 20 14:47:09 2012 -0400 @@ -0,0 +1,80 @@ +#!/usr/bin/perl +#------------------------------------------------------------------------------- +# +# Plot a QQ plot (using R) +# Data is feed as a 1 column of numbers +# +# Note: Any line that does not match a numeric regular expression, is filtered out). +# +# Pablo Cingolani +#------------------------------------------------------------------------------- + +#------------------------------------------------------------------------------- +# Main +#------------------------------------------------------------------------------- + +# Parse command line option (file base name) +$base = 'QQ-plot'; +if( $ARGV[0] ne '' ) { $base = $ARGV[0]; } + +$pngFile = "$base.png"; +$txtFile = "$base.txt"; + +# Read STDIN and create an R vector +open TXT, "> $txtFile" or die "Cannot open output file '$txtFile'\n"; +print TXT "x\n"; +for( $ln = 0 ; $l = <STDIN> ; ) { + chomp $l; + + # Does the string contain exactly one number? (can be float) + if( $l =~ /^[-+]?[0-9]*\.?[0-9]+([eE][-+]?[0-9]+)?$/ ) { print TXT "$l\n"; } +} +close TXT; + +#--- +# Create an R program, save QQ-plot as PNG image +#--- + +open R, "| R --vanilla --slave " or die "Cannot open R program\n"; +print R <<EOF; + +qqplot <- function( x, title ) { + keep <- (x > 0) & (x <= 1) & ( ! is.na(x) ); + x <- x[keep] + s <- sort(x); + ly <- -log10(s); + + n <- length(s); + lx <- -log10( (1:n) / (n+1) ) + + # Show auto range + #par( mfrow=c(2,1) ); + #plot( lx, ly, main=title, xlab="-Log[ rank / (N+1) ]", ylab="-Log[ p ]" ); + #abline( 0 , 1 , col='red'); + + # Show full range in both plots + range <- c(0 , max(lx, ly) ); + plot( lx, ly, xlim=range, ylim=range, main=title, xlab="-Log[ rank / (N+1) ]", ylab="-Log[ p ]" ); + abline( 0 , 1 , col='red'); +} + +png('$pngFile', width = 1024, height = 1024); + +data <- read.csv("$txtFile", sep='\t', header = TRUE); +qqplot( data\$x, "$base" ); + +dev.off(); +quit( save='no' ) +EOF + +close R; + +#--- +# Show figure +#--- + +$os = `uname`; +$show = "eog"; +if( $os =~ "Darwin" ) { $show = "open"; } +`$show $pngFile`; +