# HG changeset patch # User greg # Date 1450316140 18000 # Node ID d31ef9abce7475998ee2e04eb817cdaddf124dd9 # Parent 34d134cd4ca9aad830ccacf6233788cbd3e89cf1 Uploaded diff -r 34d134cd4ca9 -r d31ef9abce74 repmatch_gff3.xml --- a/repmatch_gff3.xml Wed Dec 16 14:45:32 2015 -0500 +++ b/repmatch_gff3.xml Wed Dec 16 20:35:40 2015 -0500 @@ -127,6 +127,35 @@ * **Lower Limit** - Lower limit for the Crick-Watson distance filter. * **Upper Limit** - Upper limit for the Crick-Watson distance filter. +----- + +**Output Data Files** + + * **Data MP** - gff file consisting of only peak pairs + + - Columns are **chr**, **script**, **blank**, **peak start**, **peak end**, **blank**, **normalized tag counts**, **blank** and **info**. + - Peak start and end are separated by one coordinate. + - Normalized tag is the occupancy averaged across replicates. + - Attributes include C-W distance, sum total of tag counts, number of replicates merged. + + * **Data D** - tabular file consisting of the list of all matched replicates. + * **Data UP** - tabular file consisting of all unmatched peak-pairs. + +**Output Statistics Files** + + * **Statistics Table** - tabular file providing the description key of **Data D**. + * **Statistics Histogram** - graph of the number of matched locations having the indicated replicate counts. + +**Comments on Replicates** + +Three types of replicates may be considered. Biological replicates represent independently collected biological samples. +At least two biological replicate must be performed for each experiment from which a conclusion is being drawn, and the +conclusion must be evident in both biological replicates when analyzed separately. Technical replicates represent a re-run +of the assay on the same biological material. This is usually done when one replicate fails to produce quality data, and is +used to replace that earlier replicate. Sequencing replicates represent additional sequencing of the same successful library +in order to obtain more reads should the analysis require it. The reads from individual sequencing replicates are usually +merged without need for separate analysis. +