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64
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1 #!/usr/bin/env python
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2 import argparse
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3 import os
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4 import shutil
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5 import subprocess
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6 import sys
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7 import tempfile
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8
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9 BUFF_SIZE = 1048576
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10 OUTPUT_DIR = 'phylogenomicsAnalysis_dir'
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11
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12 parser = argparse.ArgumentParser()
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13
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14 parser.add_argument('--orthogroup_faa', dest='orthogroup_faa', help="Input dataset files_path")
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15 parser.add_argument('--scaffold', dest='scaffold', default='mode', help='Orthogroups or gene families proteins scaffold')
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16 parser.add_argument('--method', dest='method', help='Protein clustering method')
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17 parser.add_argument('--config_dir', dest='config_dir', help='Directory containing default configuration files')
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18 parser.add_argument('--num_threads', dest='num_threads', type=int, help='Number of threads to use for execution')
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19 parser.add_argument('--orthogroup_fna', dest='orthogroup_fna', default=None, help="Flag for coding sequences associated with orthogroups")
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20 parser.add_argument('--sequence_type', dest='sequence_type', default=None, help="Sequence type used in the phylogenetic inference")
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21 parser.add_argument('--alignments_method', dest='alignments_method', default=None, help='Multiple sequence alignments method')
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22 parser.add_argument('--pasta_script_path', dest='pasta_script_path', default=None, help='Path to script for executing pasta')
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23 parser.add_argument('--pasta_iter_limit', dest='pasta_iter_limit', type=int, default=None, help='"Maximum number of iteration that the PASTA algorithm will execute')
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68
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24 parser.add_argument('--tree_inference', dest='tree_inference', default=None, help='Phylogenetic trees inference method')
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64
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25 parser.add_argument('--rooting_order', dest='rooting_order', default=None, help='Rooting order configuration for rooting trees')
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26 parser.add_argument('--bootstrap_replicates', dest='bootstrap_replicates', type=int, default=None, help='Number of replicates for rapid bootstrap analysis')
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27 parser.add_argument('--max_orthogroup_size', dest='max_orthogroup_size', type=int, default=None, help='Maximum number of sequences in orthogroup alignments')
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28 parser.add_argument('--min_orthogroup_size', dest='min_orthogroup_size', type=int, default=None, help='Minimum number of sequences in orthogroup alignments')
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29 parser.add_argument('--remove_sequences', dest='remove_sequences', default=None, type=float, help='Remove sequences with gaps of')
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30 parser.add_argument('--trim_type', dest='trim_type', default=None, help='Process used for gap trimming')
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31 parser.add_argument('--gap_trimming', dest='gap_trimming', default=None, type=float, help='Remove sites in alignments with gaps of')
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32 parser.add_argument('--output_ptortho', dest='output_ptortho', default=None, help='Output for orthogroups')
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33 parser.add_argument('--output_ptortho_dir', dest='output_ptortho_dir', default=None, help='output_ptortho.files_path')
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34 parser.add_argument('--output_ptorthocs', dest='output_ptorthocs', default=None, help='Output for orthogroups with corresponding coding sequences')
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35 parser.add_argument('--output_ptorthocs_dir', dest='output_ptorthocs_dir', default=None, help='output_ptorthocs.files_path')
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36 parser.add_argument('--output_aln', dest='output_aln', default=None, help='Output for orthogroups alignments')
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37 parser.add_argument('--output_aln_dir', dest='output_aln_dir', default=None, help='output_aln.files_path')
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38 parser.add_argument('--output_tree', dest='output_tree', default=None, help='Output for phylogenetic trees')
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39 parser.add_argument('--output_tree_dir', dest='output_tree_dir', default=None, help='output_tree.files_path')
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40
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41 args = parser.parse_args()
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42
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43
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44 def get_stderr_exception(tmp_err, tmp_stderr, tmp_out, tmp_stdout, include_stdout=False):
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45 tmp_stderr.close()
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46 # Get stderr, allowing for case where it's very large.
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47 tmp_stderr = open(tmp_err, 'rb')
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48 stderr_str = ''
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49 buffsize = BUFF_SIZE
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50 try:
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51 while True:
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52 stderr_str += tmp_stderr.read(buffsize)
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53 if not stderr_str or len(stderr_str) % buffsize != 0:
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54 break
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55 except OverflowError:
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56 pass
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57 tmp_stderr.close()
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58 if include_stdout:
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59 tmp_stdout = open(tmp_out, 'rb')
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60 stdout_str = ''
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61 buffsize = BUFF_SIZE
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62 try:
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63 while True:
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64 stdout_str += tmp_stdout.read(buffsize)
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65 if not stdout_str or len(stdout_str) % buffsize != 0:
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66 break
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67 except OverflowError:
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68 pass
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69 tmp_stdout.close()
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70 if include_stdout:
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71 return 'STDOUT\n%s\n\nSTDERR\n%s\n' % (stdout_str, stderr_str)
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72 return stderr_str
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73
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74
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75 def move_directory_files(source_dir, destination_dir):
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76 source_directory = os.path.abspath(source_dir)
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77 destination_directory = os.path.abspath(destination_dir)
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78 if not os.path.isdir(destination_directory):
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79 os.makedirs(destination_directory)
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80 for dir_entry in os.listdir(source_directory):
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81 source_entry = os.path.join(source_directory, dir_entry)
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82 shutil.move(source_entry, destination_directory)
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83
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84
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85 def stop_err(msg):
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86 sys.stderr.write(msg)
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87 sys.exit(1)
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88
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89
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90 def write_html_output(output, title, dir):
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91 with open(output, 'w') as fh:
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92 fh.write('<html><head><h3>%s</h3></head>\n' % title)
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93 fh.write('<body><p/><table cellpadding="2">\n')
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94 fh.write('<tr><th>Size</th><th>Name</th></tr>\n')
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95 for index, fname in enumerate(sorted(os.listdir(dir))):
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96 if index % 2 == 0:
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97 bgcolor = '#D8D8D8'
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98 else:
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99 bgcolor = '#FFFFFF'
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100 try:
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101 size = str(os.path.getsize(os.path.join(dir, fname)))
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102 except:
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103 size = 'unknown'
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104 link = '<a href="%s" type="text/plain">%s</a>\n' % (fname, fname)
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105 fh.write('<tr bgcolor="%s"><td>%s</td><td>%s</td></tr>\n' % (bgcolor, size, link))
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106 fh.write('</table></body></html>\n')
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107
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108
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109 # Define command response buffers.
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110 tmp_out = tempfile.NamedTemporaryFile().name
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111 tmp_stdout = open(tmp_out, 'wb')
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112 tmp_err = tempfile.NamedTemporaryFile().name
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113 tmp_stderr = open(tmp_err, 'wb')
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114 # Build the command line.
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115 cmd = 'PhylogenomicsAnalysis'
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116 cmd += ' --orthogroup_faa %s' % args.orthogroup_faa
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117 cmd += ' --scaffold %s' % args.scaffold
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118 cmd += ' --method %s' % args.method
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119 cmd += ' --config_dir %s' % args.config_dir
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120 cmd += ' --num_threads %d' % args.num_threads
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121
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122 if args.orthogroup_fna is not None:
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123 cmd += ' --orthogroup_fna'
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124 if args.sequence_type is not None:
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125 cmd += ' --sequence_type %s' % args.sequence_type
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126 if args.alignments_method is not None:
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127 if args.alignments_method == 'create_alignments':
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128 cmd += ' --create_alignments'
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129 elif args.alignments_method == 'add_alignments':
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130 cmd += ' --add_alignments'
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131 elif args.alignments_method == 'pasta_alignments':
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132 cmd += ' --pasta_alignments'
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133 cmd += ' --pasta_script_path %s' % args.pasta_script_path
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134 cmd += ' --pasta_iter_limit %d' % args.pasta_iter_limit
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135 if args.tree_inference is not None:
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136 cmd += ' --tree_inference %s' % args.tree_inference
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137 if args.tree_inference == 'raxml':
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138 if args.rooting_order is not None:
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139 cmd += ' --rooting_order %s' % args.rooting_order
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140 cmd += ' --bootstrap_replicates %d' % args.bootstrap_replicates
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141 if args.max_orthogroup_size is not None:
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142 cmd += ' --max_orthogroup_size %d' % args.max_orthogroup_size
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143 if args.min_orthogroup_size is not None:
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144 cmd += ' --min_orthogroup_size %d' % args.min_orthogroup_size
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145 if args.remove_sequences is not None:
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146 cmd += ' --remove_sequences %4f' % args.remove_sequences
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147 if args.trim_type is not None:
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148 if args.trim_type == 'automated_trimming':
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149 cmd += ' --automated_trimming'
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150 else:
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151 cmd += ' --gap_trimming %4f' % args.gap_trimming
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152 # Run the command.
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153 proc = subprocess.Popen(args=cmd, stderr=tmp_stderr, stdout=tmp_stdout, shell=True)
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154 rc = proc.wait()
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155 # Handle execution errors.
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156 if rc != 0:
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157 error_message = get_stderr_exception(tmp_err, tmp_stderr, tmp_out, tmp_stdout)
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158 stop_err( error_message )
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159 # Handle phylogenies for orthogroups outputs.
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160 if args.orthogroup_fna is not None:
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161 out_file = args.output_ptorthocs
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162 orthogroups_dest_dir = args.output_ptorthocs_dir
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163 title = 'Phylogenies files for orthogroups and corresponding coding sequences'
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164 else:
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165 out_file = args.output_ptortho
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166 orthogroups_dest_dir = args.output_ptortho_dir
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167 title = 'Phylogenies files for orthogroups'
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168 orthogroups_src_dir = os.path.join(OUTPUT_DIR, 'orthogroups_fasta')
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169 move_directory_files(orthogroups_src_dir, orthogroups_dest_dir)
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170 write_html_output(out_file, title, orthogroups_dest_dir)
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171 # Handle multiple sequences alignments for orthogroups outputs.
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172 if args.output_aln is not None:
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173 alignments_src_dir = os.path.join(OUTPUT_DIR, 'orthogroups_aln')
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174 alignments_dest_dir = args.output_aln_dir
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175 title = 'Multiple sequence alignments files for orthogroups'
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176 move_directory_files(alignments_src_dir, alignments_dest_dir)
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177 write_html_output(args.output_aln, title, alignments_dest_dir)
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178 # Handle phylogenies for orthogroups outputs.
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179 if args.output_tree is not None:
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180 trees_src_dir = os.path.join(OUTPUT_DIR, 'orthogroups_tree')
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181 trees_dest_dir = args.output_tree_dir
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182 title = 'Phylogenetic tree files for orthogroups'
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183 move_directory_files(trees_src_dir, trees_dest_dir)
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184 write_html_output(args.output_tree, title, trees_dest_dir)
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