Mercurial > repos > greg > bam_to_scidx
view bam_to_scidx.py @ 1:24a8165055bd draft default tip
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author | greg |
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date | Wed, 02 Dec 2015 16:13:17 -0500 |
parents | 962b0366a980 |
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""" bam_to_scidx.py Input: BAM file Output: Converted scidx file """ import optparse import os import shutil import subprocess import sys import tempfile BUFF_SIZE = 1048576 def get_stderr_exception(tmp_err, tmp_stderr): """ Return a stderr string of reasonable size. """ tmp_stderr.close() # Get stderr, allowing for case where it's very large. tmp_stderr = open(tmp_err, 'rb') stderr_str = '' buffsize = BUFF_SIZE try: while True: stderr_str += tmp_stderr.read(buffsize) if not stderr_str or len(stderr_str) % buffsize != 0: break except OverflowError: pass tmp_stderr.close() return stderr_str def stop_err(msg): sys.stderr.write(msg) sys.exit(1) parser = optparse.OptionParser() parser.add_option('-j', '--jar_file', dest='jar_file', type='string', help='BAMtoIDX.jar') parser.add_option('-b', '--input_bam', dest='input_bam', type='string', help='Input dataset in BAM format') parser.add_option('-i', '--input_bai', dest='input_bai', type='string', help='Input dataset index') parser.add_option('-r', '--read', dest='read', type='int', default=0, help='Reads.') parser.add_option('-o', '--output', dest='output', type='string', help='Output dataset in scidx format') options, args = parser.parse_args() tmp_dir = tempfile.mkdtemp(prefix='tmp-scidx-') tmp_out = tempfile.NamedTemporaryFile().name tmp_stdout = open(tmp_out, 'wb') tmp_err = tempfile.NamedTemporaryFile().name tmp_stderr = open(tmp_err, 'wb') # Link input BAM file and associated index file into the working directory. input_data_file_name = "input.bam" os.link(options.input_bam, os.path.join(tmp_dir, input_data_file_name)) os.link(options.input_bai, os.path.join(tmp_dir, "%s.bai" % input_data_file_name)) cmd = "java -jar %s -b %s -s %d -o %s" % (options.jar_file, input_data_file_name, options.read, options.output) proc = subprocess.Popen(args=cmd, stdout=tmp_stdout, stderr=tmp_stderr, shell=True, cwd=tmp_dir) return_code = proc.wait() if return_code != 0: error_message = get_stderr_exception(tmp_err, tmp_stderr) stop_err(error_message) if tmp_dir and os.path.exists(tmp_dir): shutil.rmtree(tmp_dir)