Mercurial > repos > galaxyp > custom_pro_db
comparison customProDB.R @ 6:61e45c111ef7 draft
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/customProDB commit 98f3323d4b92a4a3668788b3e1bad1a6529fd6df-dirty
author | galaxyp |
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date | Wed, 03 Feb 2016 12:37:47 -0500 |
parents | 7e078d4e40f8 |
children | b83a4002aab1 |
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5:6db27bef602f | 6:61e45c111ef7 |
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15 | 15 |
16 | 16 |
17 option_list <- list() | 17 option_list <- list() |
18 | 18 |
19 option_list$bam <- make_option('--bam', type='character') | 19 option_list$bam <- make_option('--bam', type='character') |
20 option_list$bai <- make_option('--bai', type='character') | |
20 option_list$vcf <- make_option('--vcf', type='character') | 21 option_list$vcf <- make_option('--vcf', type='character') |
21 option_list$exon_anno <- make_option('--exon_anno', type='character') | 22 option_list$exon_anno <- make_option('--exon_anno', type='character') |
22 option_list$proteinseq <- make_option('--proteinseq', type='character') | 23 option_list$proteinseq <- make_option('--proteinseq', type='character') |
23 option_list$procodingseq <- make_option('--procodingseq', type='character') | 24 option_list$procodingseq <- make_option('--procodingseq', type='character') |
25 option_list$ids <- make_option('--ids', type='character') | |
24 option_list$outputFile <- make_option('--outputFile', type='character') | 26 option_list$outputFile <- make_option('--outputFile', type='character') |
25 | 27 |
26 | 28 |
27 opt <- parse_args(OptionParser(option_list=option_list)) | 29 opt <- parse_args(OptionParser(option_list=option_list)) |
28 | 30 |
29 | 31 |
30 customProDB <- function( | 32 customProDB <- function( |
31 bam_file = GalaxyInputFile(required=TRUE), | 33 bam_file = GalaxyInputFile(required=TRUE), |
34 bai_file = GalaxyInputFile(required=TRUE), | |
32 vcf_file = GalaxyInputFile(required=TRUE), | 35 vcf_file = GalaxyInputFile(required=TRUE), |
33 exon_anno_file = GalaxyInputFile(required=TRUE), | 36 exon_anno_file = GalaxyInputFile(required=TRUE), |
34 proteinseq_file = GalaxyInputFile(required=TRUE), | 37 proteinseq_file = GalaxyInputFile(required=TRUE), |
35 procodingseq_file = GalaxyInputFile(required=TRUE), | 38 procodingseq_file = GalaxyInputFile(required=TRUE), |
39 ids_file = GalaxyInputFile(required=TRUE), | |
36 outputFile = GalaxyOutput("FASTA","fasta")) | 40 outputFile = GalaxyOutput("FASTA","fasta")) |
37 { | 41 { |
38 if (dirname(exon_anno_file) != dirname(proteinseq_file) || | 42 file.symlink(exon_anno_file, paste(dirname(exon_anno_file), "exon_anno.RData", sep="/")) |
39 dirname(exon_anno_file) != dirname(procodingseq_file)) | 43 file.symlink(proteinseq_file, paste(dirname(exon_anno_file), "proseq.RData", sep="/")) |
40 { | 44 file.symlink(procodingseq_file, paste(dirname(exon_anno_file), "procodingseq.RData", sep="/")) |
41 gstop("parent directory of annotation files must all be the same") | 45 file.symlink(ids_file, paste(dirname(exon_anno_file), "ids.RData", sep="/")) |
42 } | 46 |
43 | 47 bamLink = paste(dirname(bam_file), "input.bam", sep="/") |
44 if (file.exists(outputFile)) | 48 file.symlink(bam_file, bamLink) |
45 { | 49 file.symlink(bai_file, paste(bamLink, ".bai", sep="")) |
46 if (file.info(outputFile)$size > 0) { gstop("output file already exists") } | |
47 else | |
48 { | |
49 tryCatch( | |
50 { | |
51 file.remove(outputFile) | |
52 }, error=function(err) | |
53 { | |
54 gstop("failed to remove empty existing file") | |
55 }) | |
56 } | |
57 } | |
58 | 50 |
59 suppressPackageStartupMessages(library(customProDB)) | 51 suppressPackageStartupMessages(library(customProDB)) |
60 | 52 |
61 easyRun(bamFile=bamFile, vcfFile=vcfFile, annotation_path=dirname(exon_anno_file), outfile_path=dirname(outputFile), outfile_name=basename(outputFile)) | 53 easyRun(bamFile=bamLink, vcfFile=vcf_file, annotation_path=dirname(exon_anno_file), |
54 outfile_path=".", outfile_name="output", | |
55 nov_junction=F, INDEL=T, lablersid=F, COSMIC=F) | |
62 } | 56 } |
63 | 57 |
64 | 58 |
65 params <- list() | 59 params <- list() |
66 for(param in names(opt)) | 60 for(param in names(opt)) |