2
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1 <tool id="rgGSEAcolumns" name="Gene set enrichment" version="0.05">
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2 <description>using a generic tabular file</description>
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3 <requirements>
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3
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4 <requirement type="package" version="2.0.12">gsea_jar</requirement>
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2
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5 <requirement type="set_environment">GSEAJAR_PATH</requirement>
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6 </requirements>
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7 <command interpreter="python">rgGSEA.py --input_tab "$input1" --adjpvalcol "$adjpvalcol" --signcol "$signcol"
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8 --idcol "$idcol" --outhtml "$html_file" --input_name "${input1.name}"
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9 --setMax "$setMax" --setMin "$setMin" --nPerm "$nPerm" --plotTop "$plotTop"
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10 --gsea_jar "\$GSEAJAR_PATH"
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11 --output_dir "$html_file.files_path" --mode "$mode" --title "$title"
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12 #if $makeTab.value=='Yes'
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13 --outtab_pos "$outtab_pos" --outtab_neg "$outtab_neg"
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14 #end if
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15 #if $gmtSource.refgmtSource == "indexed" or $gmtSource.refgmtSource == "both":
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16 --builtin_gmt "${gmtSource.builtinGMT.fields.path}"
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17 #end if
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18 #if $gmtSource.refgmtSource == "history" or $gmtSource.refgmtSource == "both":
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19 --history_gmt "${gmtSource.ownGMT}" --history_gmt_name "${gmtSource.ownGMT.name}"
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20 #end if
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21 #if $chipSource.refchipSource=="builtin"
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22 --chip "${chipSource.builtinChip.fields.path}"
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23 #end if
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24 #if $chipSource.refchipSource=="history"
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25 --chip "${chipSource.ownChip}"
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26 #end if
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2
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27 </command>
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28 <inputs>
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29 <param name="input1" type="data" format="tabular" label="Select a tab delimited file with a probe id, adjusted p value and a signed weight (eg t-test statistic) on each row"
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30 help=""/>
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31 <param name="adjpvalcol" label="Column containing a p value for the DEG statistical test"
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32 help = "Use RAW p-values - not FDR adjusted as these have a non GSEA friendly non-uniform distribution"
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33 type="data_column" data_ref="input1" numerical="True"
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34 multiple="false" use_header_names="true" size="5">
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35 <validator type="no_options" message="Please select a p value column."/>
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36 </param>
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37 <param name="signcol" label="Column containing the DE sign - eg log fold change so positive/negative values = upregulated/downregulated in treatment"
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38 type="data_column" data_ref="input1" numerical="True"
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39 multiple="false" use_header_names="true" size="5">
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40 <validator type="no_options" message="Please select a sign column."/>
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41 </param>
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42 <param name="idcol" label="Column containing a gene id (refseq, symbol or Entrez"
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43 type="data_column" data_ref="input1" numerical="False"
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44 multiple="false" use_header_names="true" size="5">
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45 <validator type="no_options" message="Please select an id column."/>
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46 </param>
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47
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48 <param name="title" type="text" value="GSEA" size="80" label="Title for job outputs" help="Supply a meaningful name here to remind you what the outputs contain"/>
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49 <param name="setMin" type="integer" label="Minimum gene set size to prune (default=15)" size="5" value="15"/>
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50 <param name="setMax" type="integer" label="Maximum gene set size to prune (default=500)" size="5" value="500"/>
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51 <param name="nPerm" type="integer" label="Number of permutations (default=1000)" size="7" value="1000"/>
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52 <param name="plotTop" type="integer" label="Number of top gene sets to plot and present in detailed reports(default=20)" size="10" value="20"/>
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53 <param name="mode" type="select" label="Mode for dealing with duplicated gene ids" >
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54 <option value="Max_probe" selected="true">Use the most extreme value</option>
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55 <option value="Median_of_probes">Use the median of all supplied values</option>
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56 </param>
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57 <param name="makeTab" type="select" label="Create a tabular report containing ALL gene sets for downstream analysis" >
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58 <option value="Yes">Yes</option>
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59 <option value="No" selected="true">No</option>
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60 </param>
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61 <conditional name="chipSource" >
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62 <param name="refchipSource" type="select" label="Translate the rank file IDs (first column) using a GSEA 'chip' lookup table">
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63 <option value="builtin">Use a builtin GSEA 'chip' file</option>
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64 <option value="history">Use one of the GSEA 'chip' files in my history</option>
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65 <option value="no" selected="true">IDs are already gene symbols - no translation needed </option>
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66 </param>
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67 <when value="no">
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68 </when>
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69 <when value="builtin">
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70 <param name="builtinChip" type="select" label="Select a GSEA 'chip' to map each probe id in the input file to a gene symbol">
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71 <options from_data_table="gseaChip_3.1">
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72 <filter type="sort_by" column="2" />
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73 <validator type="no_options" message="No chip tables are available - please see the Galaxy GSEA tool documentation for instructions on installing them" />
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74 </options>
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75 </param>
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76 </when>
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77 <when value="history">
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78 <param name="ownChip" type="data" format="gseachip" label="Select an existing gseachip filefrom your history. Probe_id matching ids in your input file and a gene_symbol must be the first 2 tabular columns. Header row required."
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79 help='see docs at http://www.broadinstitute.org/cancer/software/gsea/wiki/index.php/Data_formats#CHIP:_Chip_file_format_.28.2A.chip.29'>
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80 <validator type="no_options" message="Need a gseachip datatype - tabular file, probe_id gene_symbol as first 2 columns. Header row required. Convert datatypes (pencil icon) of existing history tabular files if necessary"/>
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81 </param>
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82 </when>
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83 </conditional>
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84 <conditional name="gmtSource">
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85 <param name="refgmtSource" type="select" label="Use a gene set (.gmt) from your history or use a built-in (MSigDB etc) gene set">
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86 <option value="indexed" selected="true">Use a built-in gene set</option>
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87 <option value="history">Use a gene set from my history</option>
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88 <option value="both">Add a gene set from my history to a built in gene set</option>
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89 </param>
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90 <when value="indexed">
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91 <param name="builtinGMT" type="select" label="Select a gene set matrix (.gmt) file to use for the analysis">
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92 <options from_data_table="gseaGMT_3.1">
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93 <filter type="sort_by" column="2" />
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94 <validator type="no_options" message="No GMT v3.1 files are available - please install them"/>
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95 </options>
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96 </param>
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97 </when>
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98 <when value="history">
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99 <param name="ownGMT" type="data" format="gmt" label="Select a Gene Set from your history" />
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100 </when>
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101 <when value="both">
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102 <param name="ownGMT" type="data" format="gseagmt" label="Select a Gene Set from your history" />
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103 <param name="builtinGMT" type="select" label="Select a gene set matrix (.gmt) file to use for the analysis">
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104 <options from_data_table="gseaGMT_3.1">
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105 <filter type="sort_by" column="2" />
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106 <validator type="no_options" message="No GMT v3.1 files are available - please install them and make sure they match [tool-data]/gseaGMT_3.1.loc, editing as needed"/>
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107 </options>
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108 </param>
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109 </when>
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110 </conditional>
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111 </inputs>
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112 <outputs>
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113 <data format="html" name="html_file" label="${title}.html"/>
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114 <data format="tabular" name="outtab_pos" label="${title}_pos_allsets.xls">
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115 <filter>makeTab=="Yes"</filter>
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116 </data>
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117 <data format="tabular" name="outtab_neg" label="${title}_neg_allsets.xls">
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118 <filter>makeTab=="Yes"</filter>
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119 </data>
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120 </outputs>
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121 <tests>
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122 <test>
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123 <param name="input1" value="gsea_test_DGE.xls"/>
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124 <param name="ownGMT" value="gsea_test_DGE.xls"/>
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125 <param name="adjpvalcol" value="5"/>
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126 <param name="signcol" value="2"/>
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127 <param name="idcol" value="1"/>
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128 <param name="plotTop" value="2"/>
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129 <param name="makeTab" value="No"/>
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130 <param name="refchipSource" value="no"/>
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131 <param name="refgmtSource" value="history"/>
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132 <param name="html_file" value="gseatestout.html">
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133 <assert_contents>
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134 <has_text text="GSEA Report for Dataset" />
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135 <has_text text="enrichment results in html"/>
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136 </assert_contents>
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137 </param>
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138 <param name="setMax" value="500"/>
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139 <param name="setMin" value="15"/>
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140 <param name="nPerm" value="100"/>
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141 <param name="output_dir" value="gseatestout"/>
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142 <param name="mode" value="Max_probe"/>
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143 <param name="title" value="GSEA test"/>
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144 <param name="builtin_gmt" value="gseatestdata.gmt"/>
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145 </test>
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146 </tests>
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147 <help>
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148 **What it does**
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149
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150 Performs Gene set enrichment analysis using GSEA_ from the Broad as described in 2005Paper_ and v3.1 of msigdb
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151
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152 Refer to http://www.pnas.org/cgi/content/abstract/0506580102v1 for details of the method.
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153
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154 **Input**
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155
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156 A tabular file with a header row and some columns containing probe ids or gene names; a p value or t-test statistic reflecting the
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157 strength and a signed quantity such as log fold change or a t-test statistic reflecting the direction of differential gene expression from edgeR for counts
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158 or some other valid analysis.
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159 Probe names can be translated using the chip file option (see below)
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160 A filter has been added to remove NA and null id rows as these can't be used. A summary will appear in stdout in the page linked from the "i" (info) button for the output
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161
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162 **Gene sets**
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163
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164 If you have your own gene sets as .gmt files (see GUIDE_ for details of format) upload them in your history and use them by nominating a history sourced gmt.
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165
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166 Alternatively, use one of the built in MSigDB_ (molecular signatures database) sets.
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167 There may be additional locally provided sets such as starBase
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168
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169 Optionally, a local gmt can be merged with a built in one if you want to see how they perform competitively
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170
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171 Select the most appropriate subset of MSigDB gene sets or use all by choosing msigdb.v3.0.symbols.
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172 A non-cancer subset called c2_c3_c5_all.v3.0.symbols is recommended if you are working with non-cancer biology.
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173
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174
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175 **Chip**
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176
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177 If your input file contains HUGO gene symbols (TEK, SLC38A1...etc) then you do not need to specify a 'chip'.
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178
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179 GSEA software allows you to specify a translation table from common microarray probe IDs to gene symbols - select the option to use a chip file and choose the one that matches your probe id's
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180 Failure to match the translation correctly will generally result in failure of the job since none of your input data will be translated properly.
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181
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182 **Output**
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183
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184 A report as html - see the GUIDE_ for a guide on interpretation
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185
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186
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187 **Attribution**
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188
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189 This Galaxy tool wrapper was written by Ross Lazarus.
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190 It executes the GSEA_ java application available using MSigDB and Chip files obtained from the GSEA_ web site
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191
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192
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193 **Chip ID samples**
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194
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195 You may be able to look for probe ids in similar form to the ones you have in the list below to find the correct GSEA 'chip' file if you do not have HUGO gene symbols
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196
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197 ::
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198
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199 AG.chip
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200 Probe Set ID Gene Symbol Gene Title
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201 11986_at --- calcium-dependent lipid-binding protein, putative
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202 11987_at --- zinc finger (C3HC4-type RING finger) family protein
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203 11988_at --- WD-40 repeat family protein
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204
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205 Agilent_Human1A.chip
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206 Probe Set ID Gene Symbol Gene Title
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207 A_23_P100001 LOC400451 hypothetical gene supported by AK075564; BC060873
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208 A_23_P100011 AP3S2 adaptor-related protein complex 3, sigma 2 subunit
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209 A_23_P10002 CEP63 centrosome protein Cep63
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210
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211 Agilent_Human1Av2.chip
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212 Probe Set ID Gene Symbol Gene Title
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213 A_23_P100001 LOC400451 hypothetical gene supported by AK075564; BC060873
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214 A_23_P100011 AP3S2 adaptor-related protein complex 3, sigma 2 subunit
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215 A_23_P10002 CEP63 centrosome protein Cep63
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216
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217 Agilent_Human1B.chip
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218 Probe Set ID Gene Symbol Gene Title
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219 A_32_P10002 TUBA2 tubulin, alpha 2
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220 A_32_P100076 RPL19 ribosomal protein L19
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221 A_32_P100100 THOC3 THO complex 3
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222
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223 Agilent_Human1_cDNA.chip
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224 Probe Set ID Gene Symbol Gene Title
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225 1 RNASEH2A ribonuclease H2, large subunit
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226 10 SF3B1 splicing factor 3b, subunit 1, 155kDa
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227 100 S100A16 S100 calcium binding protein A16
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228
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229 Agilent_HumanGenome.chip
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230 Probe Set ID Gene Symbol Gene Title
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231 A_23_P100001 LOC400451 hypothetical gene supported by AK075564; BC060873
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232 A_23_P100011 NULL NULL
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233 A_23_P100022 SV2B synaptic vesicle glycoprotein 2B
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234
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235 Agilent_Mouse_cDNA.chip
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236 Probe Set ID Gene Symbol Gene Title
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237 1000982 SLC6A9 solute carrier family 6 (neurotransmitter transporter, glycine), member 9
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238 1001007 IGF1 insulin-like growth factor 1
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239 1001011 FCNA ficolin A
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240
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241 Agilent_MouseDev.chip
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242 Probe Set ID Gene Symbol Gene Title
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243 A_65_P00523 NULL NULL
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244 A_65_P00524 NULL NULL
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245 A_65_P00525 SFTPD surfactant associated protein D
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246
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247 Agilent_MouseGenome.chip
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248 Probe Set ID Gene Symbol Gene Title
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249 A_51_P100021 NULL NULL
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250 A_51_P100034 2310075G12RIK RIKEN cDNA 2310075G12 gene
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251 A_51_P100052 SLITRK2 SLIT and NTRK-like family, member 2
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252
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253 Agilent_MouseOligo.chip
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254 Probe Set ID Gene Symbol Gene Title
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255 A_51_P100021 NULL NULL
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256 A_51_P100034 2310075G12RIK RIKEN cDNA 2310075G12 gene
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257 A_51_P100052 SLITRK2 SLIT and NTRK-like family, member 2
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258
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259 Agilent_RatGenome_G4131A.chip
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260 Probe Set ID Gene Symbol Gene Title
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261 A_42_P453055 LU Lutheran blood group (Auberger b antigen included)
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262 A_42_P453737 NULL NULL
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263 A_42_P453894 RGD1306682_PREDICTED similar to RIKEN cDNA 1810046J19 (predicted)
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264
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265 Agilent_RatOligo.chip
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266 Probe Set ID Gene Symbol Gene Title
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267 A_42_P453131 CNTN1 contactin 1
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268 A_42_P453151 TTC8_PREDICTED tetratricopeptide repeat domain 8 (predicted)
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269 A_42_P453162 NULL NULL
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270
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271 APPLERA_ABI1700.chip
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272 Probe Set ID Gene Symbol Gene Title
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273 100002 NULL NULL
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274 100003 NULL NULL
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275 100027 ZNF192 zinc finger protein 192
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276
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277 ATH1_121501.chip
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278 Probe Set ID Gene Symbol Gene Title
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279 244901_at --- ---
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280 244902_at --- ---
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281 244903_at --- ---
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282
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283 AtlasMouse1.2.chip
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284 Probe Set ID Gene Symbol Gene Title
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285 MA099 HTR1A 5-hydroxytryptamine (serotonin) receptor 1A
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286 MA015 JARID2 jumonji, AT rich interactive domain 2
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287 M443 CDKN2C cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)
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288
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289 AtlasRat1.2.chip
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290 Probe Set ID Gene Symbol Gene Title
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291 R484 TPP2 tripeptidylpeptidase II
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292 RG348 MOS v-mos moloney murine sarcoma viral oncogene homolog
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293 RG119 KCNJ1 potassium inwardly-rectifying channel, subfamily J, member 1
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294
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295 Bovine.chip
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296 Probe Set ID Gene Symbol Gene Title
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297 AFFX-BioB-3_at --- ---
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298 AFFX-BioB-5_at --- ---
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299 AFFX-BioB-M_at --- ---
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300
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301 Caltech16KMouse.chip
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302 Probe Set ID Gene Symbol Gene Title
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303 6712 CCL6 chemokine (C-C motif) ligand 6
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304 8563 ACTL7B actin-like 7b
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305 8434 1700049M11RIK RIKEN cDNA 1700049M11 gene
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306
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307 Caltech16KOligoMouse.chip
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308 Probe Set ID Gene Symbol Gene Title
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309 6712 GCA grancalcin, EF-hand calcium binding protein
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310 8563 MARVELD2 MARVEL (membrane-associating) domain containing 2
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311 8434 BC048825 NULL
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312
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313 Canine_2.chip
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314 Probe Set ID Gene Symbol Gene Title
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315 AFFX-BioB-3_at --- ---
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316 AFFX-BioB-5_at --- ---
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317 AFFX-BioB-M_at --- ---
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318
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319 Clontech_Atlas_13K.chip
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320 Probe Set ID Gene Symbol Gene Title
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321 1-A01 NULL NULL
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322 1-A02 A2M alpha-2-macroglobulin
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323 1-A03 AADAC arylacetamide deacetylase (esterase)
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324
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325 Clontech_BD_Atlas.chip
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326 Probe Set ID Gene Symbol Gene Title
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327 A01a PCP4 Purkinje cell protein 4
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328 A01b NULL NULL
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329 A01c PMP22 peripheral myelin protein
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330
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331 CNMCMuscleChip.chip
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332 Probe Set ID Gene Symbol Gene Title
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333 HSPD4570_at MBP myelin basic protein
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334 HSPD7328_at MAPK12 mitogen-activated protein kinase 12
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335 HSPD17028_i_at DIA1 diaphorase (NADH) (cytochrome b-5 reductase)
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336
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337 CodeLink_Human_Whole_Genome.chip
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338 Probe Set ID Gene Symbol Gene Title
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339 GE469530 NULL NULL
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340 GE469548 TMEM1 transmembrane protein 1
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341 GE469549 NULL NULL
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342
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343 CodeLink_UniSet_Human_20K_I_Bioarray.chip
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344 Probe Set ID Gene Symbol Gene Title
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345 000106CB1_PROBE1 NDUFA4 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa
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346 000136CB1_PROBE1 MRPS33 mitochondrial ribosomal protein S33
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347 000278CB1_PROBE1 FDFT1 farnesyl-diphosphate farnesyltransferase 1
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348
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349 CodeLink_UniSet_Human_I_Bioarray.chip
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350 Probe Set ID Gene Symbol Gene Title
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351 AA001334_PROBE1 ZHX2 zinc fingers and homeoboxes 2
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352 AA002191_PROBE1 PITPNA phosphatidylinositol transfer protein, alpha
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353 AA004381_PROBE1 NULL NULL
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354
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355 CodeLink_UniSet_Human_II_Bioarray.chip
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356 Probe Set ID Gene Symbol Gene Title
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357 000106CB1_PROBE1 NDUFA4 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa
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358 000136CB1_PROBE1 MRPS33 mitochondrial ribosomal protein S33
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359 000278CB1_PROBE1 FDFT1 farnesyl-diphosphate farnesyltransferase 1
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360
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361 CodeLink_UniSet_Rat_I_Bioarray.chip
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362 Probe Set ID Gene Symbol Gene Title
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363 AA799301_PROBE1 NULL NULL
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364 AA799313_PROBE1 RGD:1303279 sialyltransferase 10 (alpha-2,3-sialyltransferase VI)
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365 AA799329_PROBE1 NULL NULL
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366
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367 DrosGenome1.chip
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368 Probe Set ID Gene Symbol Gene Title
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369 141200_at CG9418 CG9418
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370 141201_at CG17840 CG17840
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371 141202_at QKR58E-3 quaking related 58E-3
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372
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373 Drosophila_2.chip
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374 Probe Set ID Gene Symbol Gene Title
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375 1616608_a_at GPDH Glycerol 3 phosphate dehydrogenase
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376 1622892_s_at CG33057 /// MKG-P CG33057 /// monkey king protein
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377 1622893_at IM3 /// RPS10B Immune induced molecule 3 /// Ribosomal protein S10b
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378
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379 G4110A.chip
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380 Probe Set ID Gene Symbol Gene Title
|
|
381 A_23_P7033 MAGEF1 melanoma antigen, family F, 1
|
|
382 A_23_P170713 A_23_P170713 ***description missing***
|
|
383 A_23_P107801 FLJ21742 hypothetical protein FLJ21742
|
|
384
|
|
385 G4110Av2.chip
|
|
386 Probe Set ID Gene Symbol Gene Title
|
|
387 A_23_P7033 MAGEF1 melanoma antigen, family F, 1
|
|
388 A_23_P170713 A_23_P170713 No description available
|
|
389 A_23_P107801 FLJ21742 hypothetical protein FLJ21742
|
|
390
|
|
391 GENE_SYMBOL.chip
|
|
392 Probe Set ID Gene Symbol Gene Title Aliases
|
|
393 A1BG A1BG alpha-1-B glycoprotein GABDKFZP686F0970ABGHYST2477A1B
|
|
394 A2M A2M alpha-2-macroglobulin DKFZP779B086S863-7ALPHA 2MFWP007CPAMD5
|
|
395 A2ML1 A2ML1 alpha-2-macroglobulin-like 1 FLJ39129DKFZP686O1010FLJ41598DKFZP686G1812FLJ25179CPAMD9DKFZP686D2011FLJ16045FLJ41607DKFZP686C1729FLJ41597DKFZP686L1821
|
|
396
|
|
397 GenosysCytokineV2.chip
|
|
398 Probe Set ID Gene Symbol Gene Title
|
|
399 M05.2 GDF11 growth differentiation factor 11
|
|
400 M22.2 AE000170 No description available
|
|
401 E06.3 IL5 interleukin 5 (colony-stimulating factor, eosinophil)
|
|
402
|
|
403 HC_G110.chip
|
|
404 Probe Set ID Gene Symbol Gene Title
|
|
405 1000_at MAPK3 mitogen-activated protein kinase 3
|
|
406 1001_at TIE1 tyrosine kinase with immunoglobulin-like and EGF-like domains 1
|
|
407 1002_f_at CYP2C19 cytochrome P450, family 2, subfamily C, polypeptide 19
|
|
408
|
|
409 HG_Focus.chip
|
|
410 Probe Set ID Gene Symbol Gene Title
|
|
411 1007_s_at DDR1 discoidin domain receptor family, member 1
|
|
412 1053_at RFC2 replication factor C (activator 1) 2, 40kDa
|
|
413 117_at HSPA6 /// LOC652878 heat shock 70kDa protein 6 (HSP70B') /// similar to heat shock 70kDa protein 6 (HSP70B)
|
|
414
|
|
415 HG_U133A_2.chip
|
|
416 Probe Set ID Gene Symbol Gene Title
|
|
417 1007_s_at DDR1 discoidin domain receptor family, member 1
|
|
418 1053_at RFC2 replication factor C (activator 1) 2, 40kDa
|
|
419 117_at HSPA6 /// LOC652878 heat shock 70kDa protein 6 (HSP70B') /// similar to heat shock 70kDa protein 6 (HSP70B)
|
|
420
|
|
421 HG_U133AAofAv2.chip
|
|
422 Probe Set ID Gene Symbol Gene Title
|
|
423 1007_s_at DDR1 discoidin domain receptor family, member 1
|
|
424 1053_at RFC2 replication factor C (activator 1) 2, 40kDa
|
|
425 117_at HSPA6 /// LOC652878 heat shock 70kDa protein 6 (HSP70B') /// similar to heat shock 70kDa protein 6 (HSP70B)
|
|
426
|
|
427 HG_U133A.chip
|
|
428 Probe Set ID Gene Symbol Gene Title
|
|
429 1007_s_at DDR1 discoidin domain receptor family, member 1
|
|
430 1053_at RFC2 replication factor C (activator 1) 2, 40kDa
|
|
431 117_at HSPA6 /// LOC652878 heat shock 70kDa protein 6 (HSP70B') /// similar to heat shock 70kDa protein 6 (HSP70B)
|
|
432
|
|
433 HG_U133B.chip
|
|
434 Probe Set ID Gene Symbol Gene Title
|
|
435 200000_s_at PRPF8 PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) /// PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
|
|
436 200001_at CAPNS1 calpain, small subunit 1 /// calpain, small subunit 1
|
|
437 200002_at RPL35 ribosomal protein L35 /// ribosomal protein L35
|
|
438
|
|
439 HG_U133_Plus_2.chip
|
|
440 Probe Set ID Gene Symbol Gene Title
|
|
441 1007_s_at DDR1 discoidin domain receptor family, member 1
|
|
442 1053_at RFC2 replication factor C (activator 1) 2, 40kDa
|
|
443 117_at HSPA6 /// LOC652878 heat shock 70kDa protein 6 (HSP70B') /// similar to heat shock 70kDa protein 6 (HSP70B)
|
|
444
|
|
445 HG_U95Av2.chip
|
|
446 Probe Set ID Gene Symbol Gene Title
|
|
447 1000_at MAPK3 mitogen-activated protein kinase 3
|
|
448 1001_at TIE1 tyrosine kinase with immunoglobulin-like and EGF-like domains 1
|
|
449 1002_f_at CYP2C19 cytochrome P450, family 2, subfamily C, polypeptide 19
|
|
450
|
|
451 HG_U95B.chip
|
|
452 Probe Set ID Gene Symbol Gene Title
|
|
453 41880_at --- Transcribed locus
|
|
454 41881_at FLJ40142 FLJ40142 protein
|
|
455 41882_at FBXO42 F-box protein 42
|
|
456
|
|
457 HG_U95C.chip
|
|
458 Probe Set ID Gene Symbol Gene Title
|
|
459 48609_r_at KIAA1333 KIAA1333
|
|
460 48610_at C20ORF102 chromosome 20 open reading frame 102
|
|
461 48612_at N4BP1 /// LOC653213 Nedd4 binding protein 1 /// similar to Nedd4 binding protein 1
|
|
462
|
|
463 HG_U95D.chip
|
|
464 Probe Set ID Gene Symbol Gene Title
|
|
465 67020_at --- Transcribed locus
|
|
466 67021_at FAM44B Family with sequence similarity 44, member B
|
|
467 67023_at --- Transcribed locus
|
|
468
|
|
469 HG_U95E.chip
|
|
470 Probe Set ID Gene Symbol Gene Title
|
|
471 67022_at PIP5K2B Phosphatidylinositol-4-phosphate 5-kinase, type II, beta
|
|
472 67024_at CASD1 CAS1 domain containing 1
|
|
473 67026_at NFASC neurofascin homolog (chicken)
|
|
474
|
|
475 HPCGGCompugenAnnotations.chip
|
|
476 Probe Set ID Gene Symbol Gene Title
|
|
477 MGI:1913066 SH3MD2 Sh3md2, SH3 multiple domains 2
|
|
478 MGI:1913099 5430413I02RIK 5430413I02Rik, RIKEN cDNA 5430413I02 gene
|
|
479 MGI:98848 TSHB Tshb, thyroid stimulating hormone, beta subunit
|
|
480
|
|
481 HT_HG_U133A.chip
|
|
482 Probe Set ID Gene Symbol Gene Title
|
|
483 1007_s_at DDR1 discoidin domain receptor family, member 1
|
|
484 1053_at RFC2 replication factor C (activator 1) 2, 40kDa
|
|
485 117_at HSPA6 /// LOC652878 heat shock 70kDa protein 6 (HSP70B') /// similar to heat shock 70kDa protein 6 (HSP70B)
|
|
486
|
|
487 Hu35KsubA.chip
|
|
488 Probe Set ID Gene Symbol Gene Title
|
|
489 AA000993_at PRDM8 PR domain containing 8
|
|
490 AA001296_s_at PHF23 PHD finger protein 23
|
|
491 AA002245_at ZC3H11A zinc finger CCCH-type containing 11A
|
|
492
|
|
493 Hu35KsubB.chip
|
|
494 Probe Set ID Gene Symbol Gene Title
|
|
495 AFFX-BioB-3_at --- ---
|
|
496 AFFX-BioB-3_st --- ---
|
|
497 AFFX-BioB-5_at --- ---
|
|
498
|
|
499 Hu35KsubC.chip
|
|
500 Probe Set ID Gene Symbol Gene Title
|
|
501 AFFX-BioB-3_at --- ---
|
|
502 AFFX-BioB-3_st --- ---
|
|
503 AFFX-BioB-5_at --- ---
|
|
504
|
|
505 Hu35KsubD.chip
|
|
506 Probe Set ID Gene Symbol Gene Title
|
|
507 AFFX-BioB-3_at --- ---
|
|
508 AFFX-BioB-3_st --- ---
|
|
509 AFFX-BioB-5_at --- ---
|
|
510
|
|
511 Hu6800.chip
|
|
512 Probe Set ID Gene Symbol Gene Title
|
|
513 A28102_at GABRA3 gamma-aminobutyric acid (GABA) A receptor, alpha 3
|
|
514 AB000114_at OMD osteomodulin
|
|
515 AB000115_at IFI44L interferon-induced protein 44-like
|
|
516
|
|
517 Illumina_Human.chip
|
|
518 Probe Set ID Gene Symbol Gene Title
|
|
519 1-A-1 BCAP31 B-cell receptor-associated protein 31
|
|
520 1-A-10 NSFL1C NSFL1 (p97) cofactor (p47)
|
|
521 1-A-11 C20ORF24 chromosome 20 open reading frame 24
|
|
522
|
|
523 ilmn_HumanHT_12_V4_0_R1_15002873_B.chip
|
|
524 Probe Set ID Gene Symbol Gene Title Entrez Gene ID
|
|
525 ILMN_1725881 LOC23117 PREDICTED: Homo sapiens KIAA0220-like protein, transcript variant 11 (LOC23117), mRNA. 23117
|
|
526 ILMN_1910180 Homo sapiens cDNA: FLJ21027 fis, clone CAE07110 -999
|
|
527 ILMN_1804174 FCGR2B PREDICTED: Homo sapiens Fc fragment of IgG, low affinity IIb, receptor (CD32) (FCGR2B), mRNA. 2213
|
|
528
|
|
529 ilmn_MouseRef_8_V2_0_R3_11278551_A.chip
|
|
530 Probe Set ID Gene Symbol Gene Title Entrez Gene ID
|
|
531 ILMN_2607609 YIF1B Mus musculus Yip1 interacting factor homolog B (S. cerevisiae) (Yif1b), mRNA. 77254
|
|
532 ILMN_1238674 2700007P21RIK Mus musculus RIKEN cDNA 2700007P21 gene (2700007P21Rik), transcript variant 2, mRNA. 212772
|
|
533 ILMN_3062534 2700007P21RIK Mus musculus RIKEN cDNA 2700007P21 gene (2700007P21Rik), transcript variant 2, mRNA. 212772
|
|
534
|
|
535 ilmn_MouseWG_6_V2_0_R3_11278593_A.chip
|
|
536 Probe Set ID Gene Symbol Gene Title Entrez Gene ID
|
|
537 ILMN_1243094 THRSP NULL -999
|
|
538 ILMN_1238674 2700007P21RIK Mus musculus RIKEN cDNA 2700007P21 gene (2700007P21Rik), transcript variant 2, mRNA. 212772
|
|
539 ILMN_2454720 2700007P21RIK Mus musculus RIKEN cDNA 2700007P21 gene (2700007P21Rik), transcript variant 2, mRNA. 212772
|
|
540
|
|
541 labonweb_human.chip
|
|
542 Probe Set ID Gene Symbol Gene Title
|
|
543 1-A1 GAPD glyceraldehyde-3-phosphate dehydrogenase
|
|
544 1-A10 BMP5 bone morphogenetic protein 5
|
|
545 1-A11 C9ORF78 chromosome 9 open reading frame 78
|
|
546
|
|
547 lymphochip.chip
|
|
548 Probe Set ID Gene Symbol Gene Title
|
|
549 1317148 TNF tumor necrosis factor (TNF superfamily, member 2)
|
|
550 814251 SLAMF1 signaling lymphocytic activation molecule family member 1
|
|
551 712899___1 AA282233 zt12d02.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone
|
|
552
|
|
553 MG_U74Av2.chip
|
|
554 Probe Set ID Gene Symbol Gene Title
|
|
555 100001_at CD3G CD3 antigen, gamma polypeptide
|
|
556 100002_at ITIH3 inter-alpha trypsin inhibitor, heavy chain 3
|
|
557 100003_at RYR1 ryanodine receptor 1, skeletal muscle
|
|
558
|
|
559 MG_U74Bv2.chip
|
|
560 Probe Set ID Gene Symbol Gene Title
|
|
561 104769_at LIMA1 LIM domain and actin binding 1
|
|
562 104770_at --- 16 days neonate heart cDNA, RIKEN full-length enriched library, clone:D830033B01 product:unclassifiable, full insert sequence
|
|
563 104771_at RAC2 RAS-related C3 botulinum substrate 2
|
|
564
|
|
565 MG_U74Cv2.chip
|
|
566 Probe Set ID Gene Symbol Gene Title
|
|
567 128520_at CCDC97 coiled-coil domain containing 97
|
|
568 128523_at A730063M14RIK RIKEN cDNA A730063M14 gene
|
|
569 128529_at A930009G19RIK RIKEN cDNA A930009G19 gene
|
|
570
|
|
571 MOE430A.chip
|
|
572 Probe Set ID Gene Symbol Gene Title
|
|
573 1415670_at COPG coatomer protein complex, subunit gamma
|
|
574 1415671_at ATP6V0D1 ATPase, H+ transporting, lysosomal V0 subunit D1
|
|
575 1415672_at GOLGA7 golgi autoantigen, golgin subfamily a, 7
|
|
576
|
|
577 MOE430B.chip
|
|
578 Probe Set ID Gene Symbol Gene Title
|
|
579 1415670_at COPG coatomer protein complex, subunit gamma
|
|
580 1415671_at ATP6V0D1 ATPase, H+ transporting, lysosomal V0 subunit D1
|
|
581 1415672_at GOLGA7 golgi autoantigen, golgin subfamily a, 7
|
|
582
|
|
583 Mouse430_2.chip
|
|
584 Probe Set ID Gene Symbol Gene Title
|
|
585 1415670_at COPG coatomer protein complex, subunit gamma
|
|
586 1415671_at ATP6V0D1 ATPase, H+ transporting, lysosomal V0 subunit D1
|
|
587 1415672_at GOLGA7 golgi autoantigen, golgin subfamily a, 7
|
|
588
|
|
589 Mouse430A_2.chip
|
|
590 Probe Set ID Gene Symbol Gene Title
|
|
591 1415670_at COPG coatomer protein complex, subunit gamma
|
|
592 1415671_at ATP6V0D1 ATPase, H+ transporting, lysosomal V0 subunit D1
|
|
593 1415672_at GOLGA7 golgi autoantigen, golgin subfamily a, 7
|
|
594
|
|
595 Mu11KsubA.chip
|
|
596 Probe Set ID Gene Symbol Gene Title
|
|
597 aa000148_s_at NIP7 nuclear import 7 homolog (S. cerevisiae)
|
|
598 AA000151_at 1200011I18RIK RIKEN cDNA 1200011I18 gene
|
|
599 aa000380_s_at TARDBP TAR DNA binding protein
|
|
600
|
|
601 Mu11KsubB.chip
|
|
602 Probe Set ID Gene Symbol Gene Title
|
|
603 AFFX-18SRNAMur/X00686_3_at --- ---
|
|
604 AFFX-18SRNAMur/X00686_5_at --- ---
|
|
605 AFFX-18SRNAMur/X00686_M_at --- ---
|
|
606
|
|
607 Mu19KsubA.chip
|
|
608 Probe Set ID Gene Symbol Gene Title
|
|
609 AFFX-18SRNAMur/X00686_3_at --- ---
|
|
610 AFFX-18SRNAMur/X00686_5_at --- ---
|
|
611 AFFX-18SRNAMur/X00686_M_at --- ---
|
|
612
|
|
613 Mu19KsubB.chip
|
|
614 Probe Set ID Gene Symbol Gene Title
|
|
615 AFFX-18SRNAMur/X00686_3_at --- ---
|
|
616 AFFX-18SRNAMur/X00686_5_at --- ---
|
|
617 AFFX-18SRNAMur/X00686_M_at --- ---
|
|
618
|
|
619 Mu19KsubC.chip
|
|
620 Probe Set ID Gene Symbol Gene Title
|
|
621 AFFX-18SRNAMur/X00686_3_at --- ---
|
|
622 AFFX-18SRNAMur/X00686_5_at --- ---
|
|
623 AFFX-18SRNAMur/X00686_M_at --- ---
|
|
624
|
|
625 MWG_Human_30K_A.chip
|
|
626 Probe Set ID Gene Symbol Gene Title
|
|
627 1-A1 IFNA8 interferon, alpha 8
|
|
628 1-A10 AGL amylo-1, 6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III)
|
|
629 1-A11 NULL NULL
|
|
630
|
|
631 MWG_Human_30K_B.chip
|
|
632 Probe Set ID Gene Symbol Gene Title
|
|
633 1-A10 ADAM11 a disintegrin and metalloproteinase domain 11
|
|
634 1-A11 NULL NULL
|
|
635 1-A12 MAST3 microtubule associated serine/threonine kinase 3
|
|
636
|
|
637 Netherland_cancer_institute_operon_human_35k.chip
|
|
638 Probe Set ID Gene Symbol Gene Title
|
|
639 H200001046 ZZZ3 zinc finger, ZZ domain containing 3 [Homo sapiens]. [Source:RefSeq;Acc:NM_015534]
|
|
640 H200011098 ZZEF1 zinc finger, ZZ-type with EF hand domain 1 [Homo sapiens]. [Source:RefSeq;Acc:NM_015113]
|
|
641 H200006223 ZYX Zyxin (Zyxin 2). [Source:Uniprot/SWISSPROT;Acc:Q15942]
|
|
642
|
|
643 Netherland_cancer_institute_operon_mouse_FOOk.chip
|
|
644 Probe Set ID Gene Symbol Gene Title
|
|
645 M300012631 ZZEF1 NULL
|
|
646 M300012632 ZZEF1 NULL
|
|
647 M200002019 ZYX Zyxin. [Source:Uniprot/SWISSPROT;Acc:Q62523]
|
|
648
|
|
649 NIA15k.chip
|
|
650 Probe Set ID Gene Symbol Gene Title
|
|
651 H3051B03 BG067146 H3051B03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA
|
|
652 H3067H02 BG068648 H3067H02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA
|
|
653 H3121E03 AK029878 NULL
|
|
654
|
|
655 OPERON_HUMANv2.chip
|
|
656 Probe Set ID Gene Symbol Gene Title
|
|
657 1-A01 NULL NULL
|
|
658 1-A02 NULL NULL
|
|
659 1-A03 NAT2 N-acetyltransferase 2 (arylamine N-acetyltransferase)
|
|
660
|
|
661 OPERON_HUMANv3.chip
|
|
662 Probe Set ID Gene Symbol Gene Title
|
|
663 1-A03 TSPAN6 tetraspanin 6
|
|
664 1-A04 AK2 adenylate kinase 2
|
|
665 1-A05 NULL NULL
|
|
666
|
|
667 RAE230A.chip
|
|
668 Probe Set ID Gene Symbol Gene Title
|
|
669 1367452_at SUMO2 SMT3 suppressor of mif two 3 homolog 2 (yeast)
|
|
670 1367453_at CDC37 cell division cycle 37 homolog (S. cerevisiae)
|
|
671 1367454_at COPB2 coatomer protein complex, subunit beta 2 (beta prime)
|
|
672
|
|
673 RAE230B.chip
|
|
674 Probe Set ID Gene Symbol Gene Title
|
|
675 1367452_at SUMO2 SMT3 suppressor of mif two 3 homolog 2 (yeast)
|
|
676 1367453_at CDC37 cell division cycle 37 homolog (S. cerevisiae)
|
|
677 1367454_at COPB2 coatomer protein complex, subunit beta 2 (beta prime)
|
|
678
|
|
679 Rat230_2.chip
|
|
680 Probe Set ID Gene Symbol Gene Title
|
|
681 1367452_at SUMO2 SMT3 suppressor of mif two 3 homolog 2 (yeast)
|
|
682 1367453_at CDC37 cell division cycle 37 homolog (S. cerevisiae)
|
|
683 1367454_at COPB2 coatomer protein complex, subunit beta 2 (beta prime)
|
|
684
|
|
685 RefSeq_human.chip
|
|
686 Probe Set ID Gene Symbol Gene Title
|
|
687 NM_030625 CXXC6 CXXC finger 6
|
|
688 NM_181776 SLC36A2 solute carrier family 36 (proton/amino acid symporter), member 2
|
|
689 NM_152616 TRIM42 tripartite motif-containing 42
|
|
690
|
|
691 RefSeq_NP_Human.chip
|
|
692 Probe Set ID Gene Symbol Gene Title
|
|
693 NP_000005 A2M alpha-2-macroglobulin
|
|
694 NP_000006 NAT2 N-acetyltransferase 2 (arylamine N-acetyltransferase)
|
|
695 NP_000007 ACADM acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
|
|
696
|
|
697 RefSeq_NP_Mouse.chip
|
|
698 Probe Set ID Gene Symbol Gene Title
|
|
699 AAF62769 NULL NULL
|
|
700 AAF62770 NULL NULL
|
|
701 AAF62771 NULL NULL
|
|
702
|
|
703 RefSeq_NP_Rat.chip
|
|
704 Probe Set ID Gene Symbol Gene Title
|
|
705 NP_007225 NULL NULL
|
|
706 NP_007226 NULL NULL
|
|
707 NP_007227 NULL NULL
|
|
708
|
|
709 Research_Genetics.chip
|
|
710 Probe Set ID Gene Symbol Gene Title
|
|
711 1-a-1 NULL NULL
|
|
712 1-a-10 WNT2 wingless-type MMTV integration site family member 2
|
|
713 1-a-11 VHL von Hippel-Lindau tumor suppressor
|
|
714
|
|
715 RG_U34A.chip
|
|
716 Probe Set ID Gene Symbol Gene Title
|
|
717 A01157cds_s_at LIPF lipase, gastric
|
|
718 A03913cds_s_at SERPINE2 serine (or cysteine) proteinase inhibitor, clade E, member 2
|
|
719 A04674cds_s_at UCP1 uncoupling protein 1 (mitochondrial, proton carrier)
|
|
720
|
|
721 RG_U34B.chip
|
|
722 Probe Set ID Gene Symbol Gene Title
|
|
723 AFFX-BioB-3_at --- ---
|
|
724 AFFX-BioB-3_st --- ---
|
|
725 AFFX-BioB-5_at --- ---
|
|
726
|
|
727 RG_U34C.chip
|
|
728 Probe Set ID Gene Symbol Gene Title
|
|
729 AA012646_i_at --- Transcribed locus
|
|
730 AA012646_r_at --- Transcribed locus
|
|
731 AA012654_at --- Transcribed locus
|
|
732
|
|
733 RN_U34.chip
|
|
734 Probe Set ID Gene Symbol Gene Title
|
|
735 A03913cds_s_at SERPINE2 serine (or cysteine) proteinase inhibitor, clade E, member 2
|
|
736 A17753cds_s_at DRD3 dopamine receptor D3
|
|
737 AA848563_s_at HSPA1A /// HSPA1B_MAPPED heat shock 70kD protein 1A /// heat shock 70kD protein 1B (mapped)
|
|
738
|
|
739 Rosetta50K.chip
|
|
740 Probe Set ID Gene Symbol Gene Title
|
|
741 1360465 RANBP9 RAN binding protein 9
|
|
742 1176829 LOC375449 similar to microtubule associated testis specific serine/threonine protein kinase
|
|
743 1351074 NEDD4L neural precursor cell expressed, developmentally down-regulated 4-like
|
|
744
|
|
745 Rosetta.chip
|
|
746 Probe Set ID Gene Symbol Gene Title
|
|
747 NM_000504 F10 coagulation factor X
|
|
748 Contig32955_RC ARL6IP6 ADP-ribosylation-like factor 6 interacting protein 6
|
|
749 AK000455 MGC16733 hypothetical gene MGC16733 similar to CG12113
|
|
750
|
|
751 RT_U34.chip
|
|
752 Probe Set ID Gene Symbol Gene Title
|
|
753 AA108277_at HSPH1 heat shock 105kDa/110kDa protein 1
|
|
754 AA108308_i_at MDM2_PREDICTED Transformed mouse 3T3 cell double minute 2 homolog (mouse) (predicted)
|
|
755 AA108308_s_at --- ---
|
|
756
|
|
757 RZPD_Human_Ensembl1.1.chip
|
|
758 Probe Set ID Gene Symbol Gene Title
|
|
759 RZPDp203A011001D SARDH sarcosine dehydrogenase
|
|
760 RZPDp203A011002D ARHGAP22 Rho GTPase activating protein 22
|
|
761 RZPDp203A011003D CNGA3 cyclic nucleotide gated channel alpha 3
|
|
762
|
|
763 RZPD_Human_ORF_Clones_Gateway.chip
|
|
764 Probe Set ID Gene Symbol Gene Title
|
|
765 RZPDo834A0110D - Gateway (closed) PTD015 PTD015 protein
|
|
766 RZPDo834A0114D - Gateway (closed) TRIB3 tribbles homolog 3 (Drosophila)
|
|
767 RZPDo834A0116D - Gateway (closed) ORM2 orosomucoid 2
|
|
768
|
|
769 RZPD_Human_Unigene3.1.chip
|
|
770 Probe Set ID Gene Symbol Gene Title
|
|
771 HU3_p983A011001D SARDH sarcosine dehydrogenase
|
|
772 HU3_p983A011002D ARHGAP22 Rho GTPase activating protein 22
|
|
773 HU3_p983A011003D CNGA3 cyclic nucleotide gated channel alpha 3
|
|
774
|
|
775 Seq_Accession.chip
|
|
776 Probe Set ID Gene Symbol
|
|
777 AA017197 C21ORF36
|
|
778 AA191116 MTVR2
|
|
779 AA280701 CXYORF7
|
|
780
|
|
781 Stanford.chip
|
|
782 Probe Set ID Gene Symbol Gene Title
|
|
783 IMAGE:703849 DDB2 damage-specific DNA binding protein 2, 48kDa
|
|
784 IMAGE:1301778 ZFY zinc finger protein, Y-linked
|
|
785 IMAGE:795810 HS.99503HS.520681 Homo sapiens transcribed sequenceHomo sapiens, clone IMAGE:4823270, mRNA
|
|
786
|
|
787 Stanford_Source_Accessions.chip
|
|
788 Probe Set ID Gene Symbol Gene Title
|
|
789 AI848107 0610010K14RIK RIKEN cDNA 0610010K14 gene
|
|
790 AK002491 0610010K14RIK RIKEN cDNA 0610010K14 gene
|
|
791 AK003842 0610010K14RIK RIKEN cDNA 0610010K14 gene
|
|
792
|
|
793 TIGR_31K_Human_Set.chip
|
|
794 Probe Set ID Gene Symbol Gene Title
|
|
795 1-1 NULL NULL
|
|
796 1-10 WNT2 wingless-type MMTV integration site family member 2
|
|
797 1-11 VHL von Hippel-Lindau tumor suppressor
|
|
798
|
|
799 TIGR_40K_Human_Set.chip
|
|
800 Probe Set ID Gene Symbol Gene Title
|
|
801 10 NULL NULL
|
|
802 100 TEX27 testis expressed sequence 27
|
|
803 1000 HOXA1 homeo box A1
|
|
804
|
|
805 U133_X3P.chip
|
|
806 Probe Set ID Gene Symbol Gene Title
|
|
807 1053_3p_at RFC2 replication factor C (activator 1) 2, 40kDa
|
|
808 117_3p_at HSPA6 /// LOC652878 heat shock 70kDa protein 6 (HSP70B') /// similar to heat shock 70kDa protein 6 (HSP70B)
|
|
809 1494_3p_f_at CYP2A6 cytochrome P450, family 2, subfamily A, polypeptide 6
|
|
810
|
|
811 UCLA_NIH_33K.chip
|
|
812 Probe Set ID Gene Symbol Gene Title
|
|
813 1020181 NULL NULL
|
|
814 1020315 VAV2 vav 2 oncogene
|
|
815 1020478 AP1GBP1 AP1 gamma subunit binding protein 1
|
|
816
|
|
817 Zebrafish.chip
|
|
818 Probe Set ID Gene Symbol Gene Title
|
|
819 AFFX-BioB-3_at --- ---
|
|
820 AFFX-BioB-5_at --- ---
|
|
821 AFFX-BioB-M_at --- ---
|
|
822
|
|
823
|
|
824 .. _LGPL: http://www.gnu.org/copyleft/lesser.html
|
|
825 .. _GSEA: http://www.broadinstitute.org/gsea
|
|
826 .. _GUIDE: http://www.broadinstitute.org/gsea/doc/GSEAUserGuideFrame.html?_Interpreting_GSEA_Results
|
|
827 .. _MSigDB: http://www.broadinstitute.org/gsea/msigdb/index.jsp
|
|
828 .. _2005Paper: http://www.pnas.org/content/102/43/15545.full
|
|
829
|
|
830 </help>
|
|
831
|
|
832 </tool>
|
|
833
|
|
834
|