Mercurial > repos > fubar > data_manager_rnasta
view data_manager/rnastar_index_builder.py @ 5:503097df1670 draft
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author | fubar |
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date | Fri, 09 Jan 2015 22:38:21 -0500 |
parents | 102bdfdda10b |
children | f9d8dc8c2152 |
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#!/usr/bin/env python #Dan Blankenberg # adapted from Dan's BWA one for rna star # ross lazarus sept 2014 # fixed some stupid bugs January 2015 import sys import os import tempfile import optparse import subprocess from galaxy.util.json import from_json_string, to_json_string CHUNK_SIZE = 2**20 ONE_GB = 2**30 # from json import loads, dumps DEFAULT_DATA_TABLE_NAME = "rnastar_index" def get_id_name( params, dbkey, fasta_description=None): #TODO: ensure sequence_id is unique and does not already appear in location file sequence_id = params['param_dict']['sequence_id'] if not sequence_id: sequence_id = dbkey sequence_name = params['param_dict']['sequence_name'] if not sequence_name: sequence_name = fasta_description if not sequence_name: sequence_name = dbkey return sequence_id, sequence_name def _run_command( command, target_directory ): tmp_stderr = tempfile.NamedTemporaryFile( prefix = "tmp-data-manager-rnastar_index_builder-stderr" ) return_code = subprocess.call( command, shell=False, stdout=sys.stdout, stderr=tmp_stderr.fileno() ) if return_code: tmp_stderr.flush() tmp_stderr.seek( 0 ) sys.stderr.write( "### star reports an error:\n" ) while True: chunk = tmp_stderr.read( CHUNK_SIZE ) if not chunk: break sys.stderr.write( chunk ) sys.exit( return_code ) tmp_stderr.close() return return_code def build_rnastar_index( data_manager_dict, fasta_filename, target_directory, dbkey, sequence_id, sequence_name, data_table_name, sjdbOverhang,sjdbGTFfile, sjdbFileChrStartEnd,sjdbGTFtagExonParentTranscript,sjdbGTFfeatureExon,sjdbGTFchrPrefix,n_threads): #TODO: allow multiple FASTA input files fasta_base_name = os.path.basename( fasta_filename ) sym_linked_fasta_filename = os.path.join( target_directory, fasta_base_name ) os.symlink( fasta_filename, sym_linked_fasta_filename ) print >> sys.stdout,'made',sym_linked_fasta_filename cl = ['STAR','--runMode', 'genomeGenerate', '--genomeFastaFiles', sym_linked_fasta_filename, '--genomeDir', target_directory, '--runThreadN',n_threads ] if sjdbGTFfile: cl += [ '--sjdbGTFfeatureExon', sjdbGTFfeatureExon,'--sjdbGTFtagExonParentTranscript',sjdbGTFtagExonParentTranscript] if (sjdbGTFchrPrefix > ''): cl += ['--sjdbGTFchrPrefix', sjdbGTFchrPrefix] cl += ['--sjdbOverhang', sjdbOverhang, '--sjdbGTFfile', sjdbGTFfile] elif sjdbFileChrStartEnd: cl += ['--sjdbFileChrStartEnd', sjdbFileChrStartEnd, '--sjdbOverhang', sjdbOverhang] tmp_stderr = tempfile.NamedTemporaryFile( prefix = "tmp-data-manager-rnastar-index-builder-stderr" ) proc = subprocess.Popen( args=cl, shell=False, cwd=target_directory, stderr=tmp_stderr.fileno() ) return_code = proc.wait() if return_code: tmp_stderr.flush() tmp_stderr.seek(0) print >> sys.stderr, "Error building index:" while True: chunk = tmp_stderr.read( CHUNK_SIZE ) if not chunk: break sys.stderr.write( chunk ) sys.exit( return_code ) tmp_stderr.close() data_table_entry = dict( value=sequence_id, dbkey=dbkey, name=sequence_name, path=fasta_base_name ) _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ) def _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ): data_manager_dict['data_tables'] = data_manager_dict.get( 'data_tables', {} ) data_manager_dict['data_tables'][ data_table_name ] = data_manager_dict['data_tables'].get( data_table_name, [] ) data_manager_dict['data_tables'][ data_table_name ].append( data_table_entry ) return data_manager_dict def main(): #Parse Command Line parser = optparse.OptionParser() parser.add_option( '--fasta_filename', dest='fasta_filename', action='store', type="string", default=None, help='fasta_filename' ) parser.add_option( '--fasta_dbkey', dest='fasta_dbkey', action='store', type="string", default=None, help='fasta_dbkey' ) parser.add_option( '--fasta_description', dest='fasta_description', action='store', type="string", default=None, help='fasta_description' ) parser.add_option( '--data_table_name', dest='data_table_name', action='store', type="string", default=None, help='data_table_name' ) parser.add_option( '--sjdbGTFfile', type="string", default=None ) parser.add_option( '--sjdbGTFchrPrefix', type="string", default=None ) parser.add_option( '--sjdbGTFfeatureExon', type="string", default=None ) parser.add_option( '--sjdbGTFtagExonParentTranscript', type="string", default=None ) parser.add_option( '--sjdbFileChrStartEnd', type="string", default=None ) parser.add_option( '--sjdbOverhang', type="string", default='100' ) parser.add_option( '--runThreadN', type="string", default='4' ) (options, args) = parser.parse_args() filename = args[0] params = from_json_string( open( filename ).read() ) target_directory = params[ 'output_data' ][0]['extra_files_path'].encode('ascii','replace') data_manager_dict = {} dbkey = options.fasta_dbkey if dbkey in [ None, '', '?' ]: raise Exception( '"%s" is not a valid dbkey. You must specify a valid dbkey.' % ( dbkey ) ) sequence_id, sequence_name = get_id_name( params, dbkey=dbkey, fasta_description=options.fasta_description ) try: os.mkdir( target_directory ) except OSError: pass #build the index build_rnastar_index( data_manager_dict = data_manager_dict, fasta_filename = options.fasta_filename, target_directory = target_directory, dbkey = dbkey, sequence_id = sequence_id, sequence_name = sequence_name, data_table_name=options.data_table_name, sjdbOverhang=options.sjdbOverhang,sjdbGTFfile=options.sjdbGTFfile, sjdbFileChrStartEnd=options.sjdbFileChrStartEnd,sjdbGTFtagExonParentTranscript=options.sjdbGTFtagExonParentTranscript, sjdbGTFfeatureExon=options.sjdbGTFfeatureExon,sjdbGTFchrPrefix=options.sjdbGTFchrPrefix, n_threads=options.runThreadN ) open( filename, 'wb' ).write( to_json_string( data_manager_dict ) ) if __name__ == "__main__": main()