# HG changeset patch # User elixir-it # Date 1569086514 14400 # Node ID a517190d2b3d1a70cd8b1accad7c5bcdc43ce6e8 # Parent 467fcccde14d2aecaf89a43c9504a487d767b847 Uploaded diff -r 467fcccde14d -r a517190d2b3d .shed.yml --- a/.shed.yml Thu Oct 25 04:44:53 2018 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,10 +0,0 @@ -categories: -- Variant Analysis -description: 'MuTect2:somatic SNP and indel caller' -homepage_url: https://software.broadinstitute.org/gatk/documentation/tooldocs/3.8-0/org_broadinstitute_gatk_tools_walkers_cancer_m2_MuTect2.php -long_description: | -MuTect2 is a somatic SNP and indel caller that combines the DREAM challenge-winning somatic genotyping engine of the original MuTect (Cibulskis et al., 2013) with the assembly-based machinery of HaplotypeCaller. -name: MuTect2 -owner: elixir-it -remote_repository_url: https://github.com/Laniakea-elixir-it/GDC-DNA-Seq-pipeline-galaxy-wrapper/tree/master/Mutect2-wrapper -type: unrestricted diff -r 467fcccde14d -r a517190d2b3d mutect2.xml --- a/mutect2.xml Thu Oct 25 04:44:53 2018 -0400 +++ b/mutect2.xml Sat Sep 21 13:21:54 2019 -0400 @@ -38,13 +38,13 @@ ##TODO creation of .dict file of the genome required by mutect2 to run #if $reference_source.reference_source_selector == "history" - java -jar \$CONDA_DEFAULT_ENV/share/picard-2.7.1-2/picard.jar CreateSequenceDictionary R= genome.fa O= genome.dict 2>$log && + java -jar \$CONDA_PREFIX/share/picard-2.7.1-2/picard.jar CreateSequenceDictionary R= genome.fa O= genome.dict 2>$log && #end if ##TODO gatk-register take the GenomeAnalysisTK-3.8-0-ge9d806836.tar.bz2 unzip it - ##and move the .jar file to \$CONDA_DEFAULT_ENV/opt/gatk-3.8/ then the mutect2 command is runned + ##and move the .jar file to \$CONDA_PREFIX/opt/gatk-3.8/ then the mutect2 command is runned gatk3-register \$_CONDA_DIR/../GenomeAnalysisTK-3.8-0-ge9d806836.tar.bz2 2>$log ; - java -jar \$CONDA_DEFAULT_ENV/opt/gatk-3.8/GenomeAnalysisTK.jar -nct 4 -T MuTect2 -I:tumor tumor.bam -I:normal normal.bam -o $output + java -jar \$CONDA_PREFIX/opt/gatk-3.8/GenomeAnalysisTK.jar -nct 4 -T MuTect2 -I:tumor tumor.bam -I:normal normal.bam -o $output #if $reference_source.reference_source_selector == "history" -R genome.fa #end if diff -r 467fcccde14d -r a517190d2b3d tool-data/mutect2_indexes.loc.sample --- a/tool-data/mutect2_indexes.loc.sample Thu Oct 25 04:44:53 2018 -0400 +++ b/tool-data/mutect2_indexes.loc.sample Sat Sep 21 13:21:54 2019 -0400 @@ -32,4 +32,3 @@ #is the value stored in the workflow for that parameter. That is why the #hg19 entry above looks odd. New genomes can be better-looking. # -hg38 hg38 hg38_GDC /export/gatkhg38pl/GRCh38.d1.vd1.fa