comparison GDC-DNA-Seq-pipeline-galaxy-wrapper.git @ 3:63456e69f310 draft

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867
868 <article class="markdown-body entry-content" itemprop="text"><h1><a id="user-content-gdc-dna-seq-pipeline-galaxy-wrapper" class="anchor" aria-hidden="true" href="#gdc-dna-seq-pipeline-galaxy-wrapper"><svg class="octicon octicon-link" viewBox="0 0 16 16" version="1.1" width="16" height="16" aria-hidden="true"><path fill-rule="evenodd" d="M4 9h1v1H4c-1.5 0-3-1.69-3-3.5S2.55 3 4 3h4c1.45 0 3 1.69 3 3.5 0 1.41-.91 2.72-2 3.25V8.59c.58-.45 1-1.27 1-2.09C10 5.22 8.98 4 8 4H4c-.98 0-2 1.22-2 2.5S3 9 4 9zm9-3h-1v1h1c1 0 2 1.22 2 2.5S13.98 12 13 12H9c-.98 0-2-1.22-2-2.5 0-.83.42-1.64 1-2.09V6.25c-1.09.53-2 1.84-2 3.25C6 11.31 7.55 13 9 13h4c1.45 0 3-1.69 3-3.5S14.5 6 13 6z"></path></svg></a>GDC-DNA-Seq-pipeline-galaxy-wrapper</h1>
869 <p>The GDC DNA-Seq analysis pipeline identifies somatic variants within whole exome sequencing (WXS) and whole genome sequencing (WGS) data. Somatic variants are identified by comparing allele frequencies in normal and tumor sample alignments, annotating each mutation, and aggregating mutations from multiple cases into one project file.</p>
870 <p>The first pipeline starts with a reference alignment step followed by co-cleaning to increase the alignment quality. Four different variant calling pipelines are then implemented separately to identify somatic mutations. Somatic-caller-identified variants are then annotated. An aggregation pipeline incorporates variants from all cases in one project into a MAF file for each pipeline.</p>
871 <p>DNA-Seq analysis is implemented across six main procedures:</p>
872 <ul>
873 <li>Genome Alignment</li>
874 <li>Alignment Co-Cleaning</li>
875 <li>Somatic Variant Calling</li>
876 <li>Variant Annotation</li>
877 <li>Mutation Aggregation</li>
878 </ul>
879 <p><strong>Genome Alignment</strong></p>
880 <p>Read groups are aligned to the reference genome BWA-MEM if mean read length is greater than or equal to 70 bp. Otherwise BWA-aln is used.
881 Each read group is aligned to the reference genome separately and all read group alignments that belong to a single aliquot are merged using SortSam and MergeSamFiles (Picard Tools).</p>
882 <p><strong>Co-cleaning Workflow</strong></p>
883 <p>Co-cleaning is performed as a separate pipeline as it uses multiple BAM files (i.e. the tumor BAM and normal tissue BAM) associated with the same patient. Both steps of this process are implemented using GATK</p>
884 <pre><code>* Local realignment of insertions and deletions is performed using IndelRealigner
885 * A base quality score recalibration (BQSR) step is then performed using BaseRecalibrator.
886 * A filter reads step is preformed by Printreads
887 </code></pre>
888 <p><strong>Somatic Variant Calling</strong></p>
889 <p>Aligned and co-cleaned BAM files are processed through the Somatic Mutation Calling Workflow as tumor-normal pairs.
890 Variant calling is performed using four separate pipelines:</p>
891 <pre><code>* MuSE
892 * MuTect2
893 * VarScan2
894 * SomaticSniper
895 </code></pre>
896 <p><strong>Variant Call Annotation Workflow</strong></p>
897 <p>Raw VCF files are then annotated in the Somatic Annotation Workflow with the Variant Effect Predictor v84
898 In addition to annotation False Positive Filter is used to label low quality variants in VarScan and SomaticSniper outputs.</p>
899 <p><strong>Somatic Agregation Workflow</strong></p>
900 <p>multiple vcf file can merged and using vcf2maf a MAF file from multiple vcf can be created.</p>
901 <p>#More informations at
902 <a href="https://docs.gdc.cancer.gov/Data/Bioinformatics_Pipelines/DNA_Seq_Variant_Calling_Pipeline/#file-access-and-availability" rel="nofollow">https://docs.gdc.cancer.gov/Data/Bioinformatics_Pipelines/DNA_Seq_Variant_Calling_Pipeline/#file-access-and-availability</a></p>
903 <p><em>All the tools and option used in the command line pipeline can be used throught galaxy wrappers</em></p>
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