# HG changeset patch # User elixir-it # Date 1541764384 18000 # Node ID 91abc25944cf2d04ef2d669b1a9bc449ec9c0300 # Parent 733c164f2759898f2173b255bc9b9e9741a1078d Uploaded diff -r 733c164f2759 -r 91abc25944cf covacs_VariantRecalibrator.xml --- a/covacs_VariantRecalibrator.xml Fri Nov 09 06:52:10 2018 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,223 +0,0 @@ - - GATK VariantRecalibrator wrapper Version = 3.8 - - bed_macros.xml - vcf_macros.xml - - - gatk - - - $log - ]]> - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -.. class:: warningmark - -**IMPORTANT** to get the wrapper ready to start the admin user have to download gatk GATK version 3.8 from the broadinstitute site https://software.broadinstitute.org/gatk/download/archive and then move it in the conda_prefix folder, the path of the conda_prefix is written in the galaxy.ini(or .yml) file - - **more informations** at https://software.broadinstitute.org/gatk/documentation/tooldocs/3.8-0/org_broadinstitute_gatk_tools_walkers_variantrecalibration_VariantRecalibrator.php - ------ - -**Implemented options** VariantRecalibrator: - -**-L** : One or more genomic intervals over which to operate(file.bed) - -**-ip** Amount of padding (in bp) to add to each interval - -**--resource:NAME,known=true/false,training=true/false,truth=true/false,prior=float $file** :A list of sites for which to apply a prior probability of being correct but which aren't used by the algorithm (training and truth sets are required to run) - -**-mode** : Recalibration mode to employ (SNP|INDEL) - -**-an** : annotations which should used for calculations - -**-tranche** The levels of truth sensitivity at which to slice the data. (in percent, that is 1.0 for 1 percent) - -**in case of indels mode** - -**--minNumBadVariants** : Minimum number of bad variants - -**--maxGaussians** : Max number of Gaussians for the positive model - -**-mNG** : Max number of Gaussians for the negative model - -**OUTPUTS** - --recalFile - --tranchesFile - ------ - -.. class:: infomark - -**Recommended CoVaCS command** - -**-ip** 100 - -**-R** genome.fa - -**-input** VCF - -**-resource**:hapmap,known=false,training=true,truth=true,prior=15.0 hapmap.vcf - -**-resource**:omni,known=false,training=true,truth=true,prior=12.0 omni.vcf - -**-resource**:1000G,known=false,training=false,truth=false,prior=8.0 1000G.vcf - -**-resource**:dbsnp,known=true,training=false,truth=false,prior=2.0 dbsnp.vcf - -**-mode** SNP - -**-an** DP **-an** QD **-an** MQ **-an** MQRankSum **-an** ReadPosRankSum **-an** FS - -**-tranche** 100.0 **-tranche** 99.5 **-tranche** 99.0 **-tranche** 98.5 **-tranche** 90.0 - - - - 10.1186/s12864-018-4508-1 - - - diff -r 733c164f2759 -r 91abc25944cf tool_data_table_conf.xml.sample --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_data_table_conf.xml.sample Fri Nov 09 06:53:04 2018 -0500 @@ -0,0 +1,17 @@ + + + + value, dbkey, name, path + +
+ + + value, dbkey, name, path + +
+ + + value, name, path + +
+