# HG changeset patch # User eduardo # Date 1438595044 14400 # Node ID 48006f05c208071acfd7eb6e7a7663b584ec8eaf # Parent a6bcb0f8f38e268570ac5e9d60ec6bddc5132f0c Deleted selected files diff -r a6bcb0f8f38e -r 48006f05c208 url2bowtie/download_align.xml --- a/url2bowtie/download_align.xml Mon Aug 03 05:43:47 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,53 +0,0 @@ - - Downloads fastq.gz, unzips, trims with cutadapt and alignes to reference - - cutadapt - bowtie2 - samtools - - - #set index_path = '' - #if str($reference_genome.source) == "history": - echo \${PATH} && bowtie2-build "$reference_genome.own_file" genome && - ln -s "$reference_genome.own_file" genome.fa && - #set index_path = 'genome' - #else: - #set index_path = $reference_genome.index.fields.path - #end if - wget -O - $file | gunzip -c | cutadapt -q 20 - | bowtie2 -q - ## index file path - -x $index_path - -U - | samtools view -b - > $output - - - - - - - - - - - - - - - - - - - - - - - - - - - - - This tool extracts reads from sra, runs cutdapt and bowtie and outputs a bam file. - - Contact Eduardo Alves at eduardoalves@abdn.ac.uk for support and bug reports. - -