view url2bowtie/tool-data/test-data2 @ 7:8accf8976cf7

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author eduardo
date Mon, 03 Aug 2015 05:36:36 -0400
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  <div class="commit commit-tease js-details-container" >
    <p class="commit-title ">
        <a href="/galaxyproject/tools-devteam/commit/d0e3412c58bd3bdc1a483a1e2f7f9c2aa5c87a1f" class="message" data-pjax="true" title="Unify and collectionify read group handling.

The reusable macro file has:

 - Defines widgets for common read group parameters.
 - Widgets can be used at top-level (e.g. ``picard_AddOrReplaceReadGroups`` where handling is required) of inputs or within a conditional (e.g. in the mappers).
 - The conditional (used by mappers) has fours modes:
    - Set according the specification (only ID required - has all parameters). Current BWA approach.
    - Set Picard-style (presumably useful for broad pipelines?) (``ID``, ``SM``, ``LB``, ``PU``, ``PL`` are all required and there are no params for ``KS``, ``PG``, or ``FO``). In terms of what is shown and what is required - this mirrors ``picard_AddOrReplaceReadGroups``.
    - Just assign the ``ID`` and do so automatically (based either on collection or standalone dataset information). No extra form elements are shown to the user. (Should this be the new default?)
    - Do not set read groups.
 - Utilities for conditionally including parameters and formatting them (eliminates many explicit conditionals in the mappers for instance).
 - An example &quot;mapper&quot; test tool which demonstrates optionally including read groups.
 - An example read group assign test tool which demonstrates unconditionally specifying macros.

In addition to unifying everything, the macro file defines enhanced ``ID``, ``SM``, and ``LB`` parameter inputs that are wrapped in a conditional. That conditional allows manual input but also allows the user to just say Galaxy should use collection information or dataset name (if input is not in a collection) to assign these parameters. This should simplify things and make simple workflows more robust, but is absolutely essential for collection-based workflows and newer tool form options that allow running a tool in parallel over many inputs.

This may look at first glance like it is really complicated - but if you focus just on the mappers for instance and treat the macro file like a library - the client interface is actually a good deal easier than doing this stuff by hand for each tool.

Updates from original version:

 - Tests pass for ``picard_AddOrReplaceReadGroups`` as well as the BWA and Bowtie tools.
 - Remove the changes from tophat - it will require more work (it is less free form and won&#39;t accept certain valid read groups arguments).
 - Deleted empty lines I had previously introduced and idented the Cheetah code (hadn&#39;t realized I could do that).

Smaller Notes:

The new approach for BWA tools excludes empty read groups instead of adding them as empty - hopefully this is fine:

```
-@RG    ID:rg1  SM:     PL:CAPILLARY
-@PG    ID:bwa  PN:bwa  VN:0.7.12-r1039 CL:bwa sampe -r @RG     ID:rg1  SM:     PL:CAPILLARY localref.fa first.sai second.sai /tmp/tmpn_I5mQ/files/000/dataset_9.dat /tmp/tmpn_I5mQ/files/000/dataset_10.dat
+@RG    ID:rg1  PL:CAPILLARY
+@PG    ID:bwa  PN:bwa  VN:0.7.12-r1039 CL:bwa sampe -r @RG     ID:rg1  PL:CAPILLARY localref.fa first.sai second.sai /tmp/tmpmN3j1j/files/000/dataset_9.dat /tmp/tmpmN3j1j/files/000/dataset_10.dat
```">Unify and collectionify read group handling.</a>
        <span class="hidden-text-expander inline"><a href="#" class="js-details-target">…</a></span>
    </p>
      <div class="commit-desc"><pre>The reusable macro file has:

 - Defines widgets for common read group parameters.
 - Widgets can be used at top-level (e.g. ``picard_AddOrReplaceReadGroups`` where handling is required) of inputs or within a conditional (e.g. in the mappers).
 - The conditional (used by mappers) has fours modes:
    - Set according the specification (only ID required - has all parameters). Current BWA approach.
    - Set Picard-style (presumably useful for broad pipelines?) (``ID``, ``SM``, ``LB``, ``PU``, ``PL`` are all required and there are no params for ``KS``, ``PG``, or ``FO``). In terms of what is shown and what is required - this mirrors ``picard_AddOrReplaceReadGroups``.
    - Just assign the ``ID`` and do so automatically (based either on collection or standalone dataset information). No extra form elements are shown to the user. (Should this be the new default?)
    - Do not set read groups.
 - Utilities for conditionally including parameters and formatting them (eliminates many explicit conditionals in the mappers for instance).
 - An example "mapper" test tool which demonstrates optionally including read groups.
 - An example read group assign test tool which demonstrates unconditionally specifying macros.

In addition to unifying everything, the macro file defines enhanced ``ID``, ``SM``, and ``LB`` parameter inputs that are wrapped in a conditional. That conditional allows manual input but also allows the user to just say Galaxy should use collection information or dataset name (if input is not in a collection) to assign these parameters. This should simplify things and make simple workflows more robust, but is absolutely essential for collection-based workflows and newer tool form options that allow running a tool in parallel over many inputs.

This may look at first glance like it is really complicated - but if you focus just on the mappers for instance and treat the macro file like a library - the client interface is actually a good deal easier than doing this stuff by hand for each tool.

Updates from original version:

 - Tests pass for ``picard_AddOrReplaceReadGroups`` as well as the BWA and Bowtie tools.
 - Remove the changes from tophat - it will require more work (it is less free form and won't accept certain valid read groups arguments).
 - Deleted empty lines I had previously introduced and idented the Cheetah code (hadn't realized I could do that).

Smaller Notes:

The new approach for BWA tools excludes empty read groups instead of adding them as empty - hopefully this is fine:

```
-<a href="https://github.com/RG" class="user-mention">@RG</a>    ID:rg1  SM:     PL:CAPILLARY
-<a href="https://github.com/PG" class="user-mention">@PG</a>    ID:bwa  PN:bwa  VN:0.7.12-r1039 CL:bwa sampe -r <a href="https://github.com/RG" class="user-mention">@RG</a>     ID:rg1  SM:     PL:CAPILLARY localref.fa first.sai second.sai /tmp/tmpn_I5mQ/files/000/dataset_9.dat /tmp/tmpn_I5mQ/files/000/dataset_10.dat
+<a href="https://github.com/RG" class="user-mention">@RG</a>    ID:rg1  PL:CAPILLARY
+<a href="https://github.com/PG" class="user-mention">@PG</a>    ID:bwa  PN:bwa  VN:0.7.12-r1039 CL:bwa sampe -r <a href="https://github.com/RG" class="user-mention">@RG</a>     ID:rg1  PL:CAPILLARY localref.fa first.sai second.sai /tmp/tmpmN3j1j/files/000/dataset_9.dat /tmp/tmpmN3j1j/files/000/dataset_10.dat
```</pre></div>
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      <a href="/galaxyproject/tools-devteam/commit/d0e3412c58bd3bdc1a483a1e2f7f9c2aa5c87a1f" class="sha-block" data-pjax>latest commit <span class="sha">d0e3412c58</span></a>

      <div class="authorship">
        <img alt="@jmchilton" class="avatar" height="20" src="https://avatars0.githubusercontent.com/u/216771?v=3&amp;s=40" width="20" />
        <span class="author-name"><a href="/jmchilton" rel="contributor">jmchilton</a></span>
        authored <time class="updated" datetime="2015-04-13T20:08:40Z" is="relative-time">Apr 13, 2015</time>

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            <span class="css-truncate css-truncate-target"><a href="/galaxyproject/tools-devteam/blob/master/tools/bowtie2/test-data/bowtie2-fq2.fq" class="js-directory-link" id="a93db4f1ea9cdfa0f62c8f46bd3aed01-ff3c56bb5eeef08c79e0c5a774aa521d9ff23ac4" title="bowtie2-fq2.fq">bowtie2-fq2.fq</a></span>
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              <a href="/galaxyproject/tools-devteam/commit/2035dc21f4c3d2a4a12b40b4cbe65714fe06f362" class="message" data-pjax="true" title="added test and test files">added test and test files</a>
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            <span class="css-truncate css-truncate-target"><time datetime="2014-12-01T15:45:35Z" is="time-ago">Dec 1, 2014</time></span>
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              <a href="/galaxyproject/tools-devteam/commit/2035dc21f4c3d2a4a12b40b4cbe65714fe06f362" class="message" data-pjax="true" title="added test and test files">added test and test files</a>
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            <span class="css-truncate css-truncate-target"><time datetime="2014-12-01T15:45:35Z" is="time-ago">Dec 1, 2014</time></span>
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            <span class="css-truncate css-truncate-target"><time datetime="2014-12-01T15:45:35Z" is="time-ago">Dec 1, 2014</time></span>
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              <a href="/galaxyproject/tools-devteam/commit/d0e3412c58bd3bdc1a483a1e2f7f9c2aa5c87a1f" class="message" data-pjax="true" title="Unify and collectionify read group handling.

The reusable macro file has:

 - Defines widgets for common read group parameters.
 - Widgets can be used at top-level (e.g. ``picard_AddOrReplaceReadGroups`` where handling is required) of inputs or within a conditional (e.g. in the mappers).
 - The conditional (used by mappers) has fours modes:
    - Set according the specification (only ID required - has all parameters). Current BWA approach.
    - Set Picard-style (presumably useful for broad pipelines?) (``ID``, ``SM``, ``LB``, ``PU``, ``PL`` are all required and there are no params for ``KS``, ``PG``, or ``FO``). In terms of what is shown and what is required - this mirrors ``picard_AddOrReplaceReadGroups``.
    - Just assign the ``ID`` and do so automatically (based either on collection or standalone dataset information). No extra form elements are shown to the user. (Should this be the new default?)
    - Do not set read groups.
 - Utilities for conditionally including parameters and formatting them (eliminates many explicit conditionals in the mappers for instance).
 - An example &quot;mapper&quot; test tool which demonstrates optionally including read groups.
 - An example read group assign test tool which demonstrates unconditionally specifying macros.

In addition to unifying everything, the macro file defines enhanced ``ID``, ``SM``, and ``LB`` parameter inputs that are wrapped in a conditional. That conditional allows manual input but also allows the user to just say Galaxy should use collection information or dataset name (if input is not in a collection) to assign these parameters. This should simplify things and make simple workflows more robust, but is absolutely essential for collection-based workflows and newer tool form options that allow running a tool in parallel over many inputs.

This may look at first glance like it is really complicated - but if you focus just on the mappers for instance and treat the macro file like a library - the client interface is actually a good deal easier than doing this stuff by hand for each tool.

Updates from original version:

 - Tests pass for ``picard_AddOrReplaceReadGroups`` as well as the BWA and Bowtie tools.
 - Remove the changes from tophat - it will require more work (it is less free form and won&#39;t accept certain valid read groups arguments).
 - Deleted empty lines I had previously introduced and idented the Cheetah code (hadn&#39;t realized I could do that).

Smaller Notes:

The new approach for BWA tools excludes empty read groups instead of adding them as empty - hopefully this is fine:

```
-@RG    ID:rg1  SM:     PL:CAPILLARY
-@PG    ID:bwa  PN:bwa  VN:0.7.12-r1039 CL:bwa sampe -r @RG     ID:rg1  SM:     PL:CAPILLARY localref.fa first.sai second.sai /tmp/tmpn_I5mQ/files/000/dataset_9.dat /tmp/tmpn_I5mQ/files/000/dataset_10.dat
+@RG    ID:rg1  PL:CAPILLARY
+@PG    ID:bwa  PN:bwa  VN:0.7.12-r1039 CL:bwa sampe -r @RG     ID:rg1  PL:CAPILLARY localref.fa first.sai second.sai /tmp/tmpmN3j1j/files/000/dataset_9.dat /tmp/tmpmN3j1j/files/000/dataset_10.dat
```">Unify and collectionify read group handling.</a>
            </span>
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            <span class="css-truncate css-truncate-target"><time datetime="2015-05-07T19:31:09Z" is="time-ago">May 7, 2015</time></span>
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