diff monocle3-diffExp.xml @ 0:9261eff5cd98 draft

"planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/ commit 8ef74a1abb7d22b5c18611b8cdd7a5bf56c51b10"

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/monocle3-diffExp.xml	Tue Mar 03 22:58:02 2020 +0000
@@ -0,0 +1,74 @@
+<?xml version="1.0" encoding="utf-8"?>
+<tool id="monocle3_diffExp" name="Monocle3 diffExp" version="@TOOL_VERSION@+galaxy0" profile="@PROFILE@">
+  <description> of genes along a trajectory</description>
+  <macros>
+    <import>monocle3-macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <command detect_errors="exit_code"><![CDATA[
+LANG=en_US.UTF-8 monocle3 diffExp
+    --neighbor-graph '$(neighbor_graph)'
+    --reduction-method '$(reduction_method)'
+#if $knn
+    --knn '$(knn)'
+#end if
+    --method '$(method)'
+    --alternative '$(alternative)'
+
+    @INPUT_OPTS@
+    output.tsv
+
+    @VERBOSE@
+]]></command>
+
+  <inputs>
+    <expand macro="input_object_params"/>
+    <param name="neighbor_graph" argument="--neighbor-graph" type="select" label="What neighbor graph to use, principal graph recommended for trajectory analysis.">
+      <option value="knn" selected="true">KNN</option>
+      <option value="principal_graph">Principal graph</option>
+    </param>
+    <param name="reduction_method" argument="--reduction-method" type="select" label="The reduction method to base the analysis on.">
+      <option value="UMAP" selected="true">UMAP</option>
+    </param>
+    <param name="knn" argument="--knn" optional="true" type="integer" value="20" label="Number of nearest neighbors used for building the kNN graph which is passed to knn2nb function during the Moran's I (Geary's C) test procedure."/>
+    <param name="method" argument="--method" type="select" label="A character string specifying the method for detecting significant genes showing correlated expression along the principal graph embedded in the low dimensional space.">
+      <option value="Moran_I" selected="true">Moran_I</option>
+    </param>
+    <param name="alternative" argument="--alternative" type="select" label="A character string specifying the alternative hypothesis.">
+      <option value="greater" selected="true">greater</option>
+      <option value="less">less</option>
+      <option value="two.sided">two-sided</option>
+    </param>
+    <expand macro="verbose_flag"/>
+  </inputs>
+
+  <outputs>
+    <data name="output_tsv" format="tsv" from_work_dir="output.tsv" label="${tool.name} on ${on_string}: tsv"/>
+  </outputs>
+
+  <tests>
+    <test>
+      <param name="input_object_file" value="input.RDS"/>
+      <param name="input_object_format" value="cds3"/>
+      <param name="neighbor_graph" value="knn"/>
+      <param name="reduction_method" value="UMAP"/>
+      <param name="knn" value="20"/>
+      <param name="method" value="Moran_I"/>
+      <param name="alternative" value="greater"/>
+      <output name="output_tsv" file="output.tsv" ftype="tsv" compare="sim_size"/>
+    </test>
+  </tests>
+
+  <help><![CDATA[
+========================================================================
+Monocle3 differential expression testing along trajectory (`graph_test`)
+========================================================================
+
+Identify differentially expressed genes along the inferred trajectory.
+
+@HELP@
+
+@VERSION_HISTORY@
+]]></help>
+  <expand macro="citations"/>
+</tool>