# HG changeset patch # User devteam # Date 1473969879 14400 # Node ID c3a5388628b510a39376adc8b951b8b1bc93636b # Parent 15de96f45d4d144b589e4c654d3cf1bb5fc95598 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfprimers commit 0b9b6512272b82637c2f1e831367e89aed77ae79 diff -r 15de96f45d4d -r c3a5388628b5 macros.xml --- a/macros.xml Thu Nov 12 10:01:54 2015 -0500 +++ b/macros.xml Thu Sep 15 16:04:39 2016 -0400 @@ -1,7 +1,7 @@ - vcflib + vcflib @@ -10,6 +10,7 @@ + 1.0.0_rc1 diff -r 15de96f45d4d -r c3a5388628b5 tool_dependencies.xml --- a/tool_dependencies.xml Thu Nov 12 10:01:54 2015 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,6 +0,0 @@ - - - - - - diff -r 15de96f45d4d -r c3a5388628b5 vcfprimers.xml --- a/vcfprimers.xml Thu Nov 12 10:01:54 2015 -0500 +++ b/vcfprimers.xml Thu Sep 15 16:04:39 2016 -0400 @@ -1,67 +1,67 @@ - - Extract flanking sequences for each VCF record - - macros.xml - - - - - #set $reference_fasta_filename = "localref.fa" - #if str( $reference_source.reference_source_selector ) == "history": - ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" && - #else: - #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path ) - #end if - vcfprimers -f "${reference_fasta_filename}" -l "${primer_length}" "${input_vcf}" > "${out_file1}" - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -For each VCF record, extract the flanking sequences, and write them as FASTA -records suitable for alignment. This tool is intended for use in designing validation -experiments. Primers extracted which would flank all of the alleles at multi-allelic -sites. The name of the FASTA "reads" indicates the VCF record which they apply to. -The form is >CHROM_POS_LEFT for the 3' primer and >CHROM_POS_RIGHT for the 5' primer, -for example:: - - >20_233255_LEFT - CCATTGTATATATAGACCATAATTTCTTTATCCAATCATCTGTTGATGGA - >20_233255_RIGHT - ACTCAGTTGATTCCATACCTTTGCCATCATGAATCATGTTGTAATAAACA - ----- - -Vcfprimers @IS_PART_OF_VCFLIB@ - - - + + Extract flanking sequences for each VCF record + + macros.xml + + + + + #set $reference_fasta_filename = "localref.fa" + #if str( $reference_source.reference_source_selector ) == "history": + ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" && + #else: + #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path ) + #end if + vcfprimers -f "${reference_fasta_filename}" -l "${primer_length}" "${input_vcf}" > "${out_file1}" + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +For each VCF record, extract the flanking sequences, and write them as FASTA +records suitable for alignment. This tool is intended for use in designing validation +experiments. Primers extracted which would flank all of the alleles at multi-allelic +sites. The name of the FASTA "reads" indicates the VCF record which they apply to. +The form is >CHROM_POS_LEFT for the 3' primer and >CHROM_POS_RIGHT for the 5' primer, +for example:: + + >20_233255_LEFT + CCATTGTATATATAGACCATAATTTCTTTATCCAATCATCTGTTGATGGA + >20_233255_RIGHT + ACTCAGTTGATTCCATACCTTTGCCATCATGAATCATGTTGTAATAAACA + +---- + +Vcfprimers @IS_PART_OF_VCFLIB@ + + +