# HG changeset patch # User devteam # Date 1444755373 14400 # Node ID 76449db9ee449791bdcc18d045133113ec48023e # Parent 5c2a97bb018be23e371de5df0dfc9e4fc50024a7 planemo upload commit 33927a87ba2eee9bf0ecdd376a66241b17b3d734 diff -r 5c2a97bb018b -r 76449db9ee44 macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Tue Oct 13 12:56:13 2015 -0400 @@ -0,0 +1,70 @@ + + + + samtools + + + + + + + @misc{SAM_def, + title={Definition of SAM/BAM format}, + url = {https://samtools.github.io/hts-specs/SAMv1.pdf},} + + 10.1093/bioinformatics/btp352 + 10.1093/bioinformatics/btr076 + 10.1093/bioinformatics/btr509 + + @misc{Danecek_et_al, + Author={Danecek, P., Schiffels, S., Durbin, R.}, + title={Multiallelic calling model in bcftools (-m)}, + url = {http://samtools.github.io/bcftools/call-m.pdf},} + + + @misc{Durbin_VCQC, + Author={Durbin, R.}, + title={Segregation based metric for variant call QC}, + url = {http://samtools.github.io/bcftools/rd-SegBias.pdf},} + + + @misc{Li_SamMath, + Author={Li, H.}, + title={Mathematical Notes on SAMtools Algorithms}, + url = {http://www.broadinstitute.org/gatk/media/docs/Samtools.pdf},} + + + @misc{SamTools_github, + title={SAMTools GitHub page}, + url = {https://github.com/samtools/samtools},} + + + + + samtools --version | head -n 1 | awk '{ print $2 }' + + + + + + + +----- + +.. class:: warningmark + +**No options available? How to re-detect metadata** + +If you see a "No options available" within the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop down, you need to re-detect metadata for the dataset you are trying to process. To do this follow these steps: + +1. Click on the **pencil** icon adjacent to the dataset in the history +2. A new menu will appear in the center pane of the interface +3. Click **Datatype** tab +4. Set **New Type** to **BAM** +5. Click **Save** + +The medatada will be re-detected and you will be able to see the list of reference sequences in the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop-down. + + + + diff -r 5c2a97bb018b -r 76449db9ee44 samtools_split.xml --- a/samtools_split.xml Fri Oct 24 16:22:16 2014 -0400 +++ b/samtools_split.xml Tue Oct 13 12:56:13 2015 -0400 @@ -1,9 +1,11 @@ - - a BAM file by read group - - samtools - - samtools --version | head -n 1 | awk '{ print $2 }' + + BAM dataset on readgroups + + macros.xml + + + + - - - - + @@ -38,16 +37,11 @@ **What it does** -This tool runs the ``samtools split`` command in the SAMtools toolkit. - -Split BAM files by read group. - -**Citation** +Splits BAM files on readgroups. -For the underlying tool, please cite `Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. <http://www.ncbi.nlm.nih.gov/pubmed/19505943>`_ - +This tool is based on ``samtools split`` command. It will generate multiple output datasets for each redagroup from the input dataset. -If you use this tool in Galaxy, please cite Blankenberg D, et al. *In preparation.* + diff -r 5c2a97bb018b -r 76449db9ee44 test-data/tmpV04boNsamtools_split_out.bam Binary file test-data/tmpV04boNsamtools_split_out.bam has changed diff -r 5c2a97bb018b -r 76449db9ee44 tool_dependencies.xml --- a/tool_dependencies.xml Fri Oct 24 16:22:16 2014 -0400 +++ b/tool_dependencies.xml Tue Oct 13 12:56:13 2015 -0400 @@ -1,6 +1,6 @@ - - + +