changeset 0:1bbde47ebd2a draft

Uploaded
author devteam
date Tue, 20 Aug 2013 12:33:44 -0400
parents
children f92f13ccee76
files samtools_flagstat.xml test-data/3unsorted.bam test-data/samtools_flagstat_out1.txt tool_dependencies.xml
diffstat 4 files changed, 54 insertions(+), 0 deletions(-) [+]
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/samtools_flagstat.xml	Tue Aug 20 12:33:44 2013 -0400
@@ -0,0 +1,37 @@
+<tool id="samtools_flagstat" name="flagstat" version="1.0.0">
+  <requirements>
+    <requirement type="package" version="0.1.18">samtools</requirement>
+  </requirements>
+  <description>provides simple stats on BAM files</description>
+  <command>samtools flagstat "$input1" > "$output1"
+  </command>
+  <inputs>
+    <param name="input1" type="data" format="bam" label="BAM File to Convert" />
+  </inputs>
+  <outputs>
+    <data name="output1" format="txt" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="input1" value="3unsorted.bam" ftype="bam" />
+      <output name="output1" file="samtools_flagstat_out1.txt" />
+    </test>
+  </tests>
+  <help>
+
+**What it does**
+
+This tool uses the SAMTools_ toolkit to produce simple stats on a BAM file.
+
+.. _SAMTools: http://samtools.sourceforge.net/samtools.shtml
+
+------
+
+**Citation**
+
+For the underlying tool, please cite `Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. &lt;http://www.ncbi.nlm.nih.gov/pubmed/19505943&gt;`_
+
+If you use this tool in Galaxy, please cite Blankenberg D, et al. *In preparation.*
+
+  </help>
+</tool>
Binary file test-data/3unsorted.bam has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/samtools_flagstat_out1.txt	Tue Aug 20 12:33:44 2013 -0400
@@ -0,0 +1,11 @@
+10 + 0 in total (QC-passed reads + QC-failed reads)
+0 + 0 duplicates
+10 + 0 mapped (100.00%:nan%)
+0 + 0 paired in sequencing
+0 + 0 read1
+0 + 0 read2
+0 + 0 properly paired (nan%:nan%)
+0 + 0 with itself and mate mapped
+0 + 0 singletons (nan%:nan%)
+0 + 0 with mate mapped to a different chr
+0 + 0 with mate mapped to a different chr (mapQ>=5)
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml	Tue Aug 20 12:33:44 2013 -0400
@@ -0,0 +1,6 @@
+<?xml version="1.0"?>
+<tool_dependency>
+    <package name="samtools" version="0.1.18">
+        <repository changeset_revision="5f7ec5048224" name="package_samtools_0_1_18" owner="devteam" toolshed="http://testtoolshed.g2.bx.psu.edu" />
+    </package>
+</tool_dependency>