# HG changeset patch
# User devteam
# Date 1377014330 14400
# Node ID cb3966035753e18981a90d8181028a4ae9d72e2f
Uploaded tool tarball.
diff -r 000000000000 -r cb3966035753 sam_pileup.py
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/sam_pileup.py Tue Aug 20 11:58:50 2013 -0400
@@ -0,0 +1,163 @@
+#!/usr/bin/env python
+
+"""
+Creates a pileup file from a bam file and a reference.
+
+usage: %prog [options]
+ -p, --input1=p: bam file
+ -o, --output1=o: Output pileup
+ -R, --ref=R: Reference file type
+ -n, --ownFile=n: User-supplied fasta reference file
+ -d, --dbkey=d: dbkey of user-supplied file
+ -x, --indexDir=x: Index directory
+ -b, --bamIndex=b: BAM index file
+ -s, --lastCol=s: Print the mapping quality as the last column
+ -i, --indels=i: Only output lines containing indels
+ -M, --mapCap=M: Cap mapping quality
+ -c, --consensus=c: Call the consensus sequence using MAQ consensu model
+ -T, --theta=T: Theta paramter (error dependency coefficient)
+ -N, --hapNum=N: Number of haplotypes in sample
+ -r, --fraction=r: Expected fraction of differences between a pair of haplotypes
+ -I, --phredProb=I: Phred probability of an indel in sequencing/prep
+
+"""
+
+import os, shutil, subprocess, sys, tempfile
+from galaxy import eggs
+import pkg_resources; pkg_resources.require( "bx-python" )
+from bx.cookbook import doc_optparse
+
+def stop_err( msg ):
+ sys.stderr.write( '%s\n' % msg )
+ sys.exit()
+
+def check_seq_file( dbkey, GALAXY_DATA_INDEX_DIR ):
+ seqFile = '%s/sam_fa_indices.loc' % GALAXY_DATA_INDEX_DIR
+ seqPath = ''
+ for line in open( seqFile ):
+ line = line.rstrip( '\r\n' )
+ if line and not line.startswith( '#' ) and line.startswith( 'index' ):
+ fields = line.split( '\t' )
+ if len( fields ) < 3:
+ continue
+ if fields[1] == dbkey:
+ seqPath = fields[2].strip()
+ break
+ return seqPath
+
+def __main__():
+ #Parse Command Line
+ options, args = doc_optparse.parse( __doc__ )
+ seqPath = check_seq_file( options.dbkey, options.indexDir )
+ # output version # of tool
+ try:
+ tmp = tempfile.NamedTemporaryFile().name
+ tmp_stdout = open( tmp, 'wb' )
+ proc = subprocess.Popen( args='samtools 2>&1', shell=True, stdout=tmp_stdout )
+ tmp_stdout.close()
+ returncode = proc.wait()
+ stdout = None
+ for line in open( tmp_stdout.name, 'rb' ):
+ if line.lower().find( 'version' ) >= 0:
+ stdout = line.strip()
+ break
+ if stdout:
+ sys.stdout.write( 'Samtools %s\n' % stdout )
+ else:
+ raise Exception
+ except:
+ sys.stdout.write( 'Could not determine Samtools version\n' )
+ #prepare file names
+ tmpDir = tempfile.mkdtemp()
+ tmpf0 = tempfile.NamedTemporaryFile( dir=tmpDir )
+ tmpf0_name = tmpf0.name
+ tmpf0.close()
+ tmpf0bam_name = '%s.bam' % tmpf0_name
+ tmpf0bambai_name = '%s.bam.bai' % tmpf0_name
+ tmpf1 = tempfile.NamedTemporaryFile( dir=tmpDir )
+ tmpf1_name = tmpf1.name
+ tmpf1.close()
+ tmpf1fai_name = '%s.fai' % tmpf1_name
+ #link bam and bam index to working directory (can't move because need to leave original)
+ os.symlink( options.input1, tmpf0bam_name )
+ os.symlink( options.bamIndex, tmpf0bambai_name )
+ #get parameters for pileup command
+ if options.lastCol == 'yes':
+ lastCol = '-s'
+ else:
+ lastCol = ''
+ if options.indels == 'yes':
+ indels = '-i'
+ else:
+ indels = ''
+ opts = '%s %s -M %s' % ( lastCol, indels, options.mapCap )
+ if options.consensus == 'yes':
+ opts += ' -c -T %s -N %s -r %s -I %s' % ( options.theta, options.hapNum, options.fraction, options.phredProb )
+ #prepare basic pileup command
+ cmd = 'samtools pileup %s -f %s %s > %s'
+ try:
+ # have to nest try-except in try-finally to handle 2.4
+ try:
+ #index reference if necessary and prepare pileup command
+ if options.ref == 'indexed':
+ if not os.path.exists( "%s.fai" % seqPath ):
+ raise Exception, "No sequences are available for '%s', request them by reporting this error." % options.dbkey
+ cmd = cmd % ( opts, seqPath, tmpf0bam_name, options.output1 )
+ elif options.ref == 'history':
+ os.symlink( options.ownFile, tmpf1_name )
+ cmdIndex = 'samtools faidx %s' % ( tmpf1_name )
+ tmp = tempfile.NamedTemporaryFile( dir=tmpDir ).name
+ tmp_stderr = open( tmp, 'wb' )
+ proc = subprocess.Popen( args=cmdIndex, shell=True, cwd=tmpDir, stderr=tmp_stderr.fileno() )
+ returncode = proc.wait()
+ tmp_stderr.close()
+ # get stderr, allowing for case where it's very large
+ tmp_stderr = open( tmp, 'rb' )
+ stderr = ''
+ buffsize = 1048576
+ try:
+ while True:
+ stderr += tmp_stderr.read( buffsize )
+ if not stderr or len( stderr ) % buffsize != 0:
+ break
+ except OverflowError:
+ pass
+ tmp_stderr.close()
+ #did index succeed?
+ if returncode != 0:
+ raise Exception, 'Error creating index file\n' + stderr
+ cmd = cmd % ( opts, tmpf1_name, tmpf0bam_name, options.output1 )
+ #perform pileup command
+ tmp = tempfile.NamedTemporaryFile( dir=tmpDir ).name
+ tmp_stderr = open( tmp, 'wb' )
+ proc = subprocess.Popen( args=cmd, shell=True, cwd=tmpDir, stderr=tmp_stderr.fileno() )
+ returncode = proc.wait()
+ tmp_stderr.close()
+ #did it succeed?
+ # get stderr, allowing for case where it's very large
+ tmp_stderr = open( tmp, 'rb' )
+ stderr = ''
+ buffsize = 1048576
+ try:
+ while True:
+ stderr += tmp_stderr.read( buffsize )
+ if not stderr or len( stderr ) % buffsize != 0:
+ break
+ except OverflowError:
+ pass
+ tmp_stderr.close()
+ if returncode != 0:
+ raise Exception, stderr
+ except Exception, e:
+ stop_err( 'Error running Samtools pileup tool\n' + str( e ) )
+ finally:
+ #clean up temp files
+ if os.path.exists( tmpDir ):
+ shutil.rmtree( tmpDir )
+ # check that there are results in the output file
+ if os.path.getsize( options.output1 ) > 0:
+ sys.stdout.write( 'Converted BAM to pileup' )
+ else:
+ stop_err( 'The output file is empty. Your input file may have had no matches, or there may be an error with your input file or settings.' )
+
+if __name__ == "__main__" : __main__()
diff -r 000000000000 -r cb3966035753 sam_pileup.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/sam_pileup.xml Tue Aug 20 11:58:50 2013 -0400
@@ -0,0 +1,190 @@
+
+ from BAM dataset
+
+ samtools
+
+
+ sam_pileup.py
+ --input1=$input1
+ --output=$output1
+ --ref=$refOrHistory.reference
+ #if $refOrHistory.reference == "history":
+ --ownFile=$refOrHistory.ownFile
+ #else:
+ --ownFile="None"
+ #end if
+ --dbkey=${input1.metadata.dbkey}
+ --indexDir=${GALAXY_DATA_INDEX_DIR}
+ --bamIndex=${input1.metadata.bam_index}
+ --lastCol=$lastCol
+ --indels=$indels
+ --mapCap=$mapCap
+ --consensus=$c.consensus
+ #if $c.consensus == "yes":
+ --theta=$c.theta
+ --hapNum=$c.hapNum
+ --fraction=$c.fraction
+ --phredProb=$c.phredProb
+ #else:
+ --theta="None"
+ --hapNum="None"
+ --fraction="None"
+ --phredProb="None"
+ #end if
+
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+
+**What it does**
+
+Uses SAMTools_' pileup command to produce a pileup dataset from a provided BAM dataset. It generates two types of pileup datasets depending on the specified options. If *Call consensus according to MAQ model?* option is set to **No**, the tool produces simple pileup. If the option is set to **Yes**, a ten column pileup dataset with consensus is generated. Both types of datasets are briefly summarized below.
+
+.. _SAMTools: http://samtools.sourceforge.net/samtools.shtml
+
+------
+
+**Types of pileup datasets**
+
+The description of pileup format below is largely based on information that can be found on SAMTools Pileup_ documentation page. The 6- and 10-column variants are described below.
+
+.. _Pileup: http://samtools.sourceforge.net/pileup.shtml
+
+**Six column pileup**::
+
+ 1 2 3 4 5 6
+ ---------------------------------
+ chrM 412 A 2 ., II
+ chrM 413 G 4 ..t, IIIH
+ chrM 414 C 4 ...a III2
+ chrM 415 C 4 TTTt III7
+
+where::
+
+ Column Definition
+ ------- ----------------------------
+ 1 Chromosome
+ 2 Position (1-based)
+ 3 Reference base at that position
+ 4 Coverage (# reads aligning over that position)
+ 5 Bases within reads where (see Galaxy wiki for more info)
+ 6 Quality values (phred33 scale, see Galaxy wiki for more)
+
+**Ten column pileup**
+
+The `ten-column` (consensus_) pileup incorporates additional consensus information generated with *-c* option of *samtools pileup* command::
+
+
+ 1 2 3 4 5 6 7 8 9 10
+ ------------------------------------------------
+ chrM 412 A A 75 0 25 2 ., II
+ chrM 413 G G 72 0 25 4 ..t, IIIH
+ chrM 414 C C 75 0 25 4 ...a III2
+ chrM 415 C T 75 75 25 4 TTTt III7
+
+where::
+
+ Column Definition
+ ------- --------------------------------------------------------
+ 1 Chromosome
+ 2 Position (1-based)
+ 3 Reference base at that position
+ 4 Consensus bases
+ 5 Consensus quality
+ 6 SNP quality
+ 7 Maximum mapping quality
+ 8 Coverage (# reads aligning over that position)
+ 9 Bases within reads where (see Galaxy wiki for more info)
+ 10 Quality values (phred33 scale, see Galaxy wiki for more)
+
+
+.. _consensus: http://samtools.sourceforge.net/cns0.shtml
+
+------
+
+**Citation**
+
+For the underlying tool, please cite `Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. <http://www.ncbi.nlm.nih.gov/pubmed/19505943>`_
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diff -r 000000000000 -r cb3966035753 test-data/chr_m.fasta
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/chr_m.fasta Tue Aug 20 11:58:50 2013 -0400
@@ -0,0 +1,335 @@
+>chrM
+GTTAATGTAGCTTAATAATATAAAGCAAGGCACTGAAAATGCCTAGATGA
+GTATTCTTACTCCATAAACACATAGGCTTGGTCCTAGCCTTTTTATTAGT
+TATTAATAGAATTACACATGCAAGTATCCGCACCCCAGTGAGAATGCCCT
+CTAAATCACGTCTCTACGATTAAAAGGAGCAGGTATCAAGCACACTAGAA
+AGTAGCTCATAACACCTTGCTCAGCCACACCCCCACGGGACACAGCAGTG
+ATAAAAATTAAGCTATGAACGAAAGTTCGACTAAGTCATATTAAATAAGG
+GTTGGTAAATTTCGTGCCAGCCACCGCGGTCATACGATTAACCCAAATTA
+ATAAATCTCCGGCGTAAAGCGTGTCAAAGACTAATACCAAAATAAAGTTA
+AAACCCAGTTAAGCCGTAAAAAGCTACAACCAAAGTAAAATAGACTACGA
+AAGTGACTTTAATACCTCTGACTACACGATAGCTAAGACCCAAACTGGGA
+TTAGATACCCCACTATGCTTAGCCCTAAACTAAAATAGCTTACCACAACA
+AAGCTATTCGCCAGAGTACTACTAGCAACAGCCTAAAACTCAAAGGACTT
+GGCGGTGCTTTACATCCCTCTAGAGGAGCCTGTTCCATAATCGATAAACC
+CCGATAAACCCCACCATCCCTTGCTAATTCAGCCTATATACCGCCATCTT
+CAGCAAACCCTAAACAAGGTACCGAAGTAAGCACAAATATCCAACATAAA
+AACGTTAGGTCAAGGTGTAGCCCATGGGATGGAGAGAAATGGGCTACATT
+TTCTACCCTAAGAACAAGAACTTTAACCCGGACGAAAGTCTCCATGAAAC
+TGGAGACTAAAGGAGGATTTAGCAGTAAATTAAGAATAGAGAGCTTAATT
+GAATCAGGCCATGAAGCGCGCACACACCGCCCGTCACCCTCCTTAAATAT
+CACAAATCATAACATAACATAAAACCGTGACCCAAACATATGAAAGGAGA
+CAAGTCGTAACAAGGTAAGTATACCGGAAGGTGTACTTGGATAACCAAAG
+TGTAGCTTAAACAAAGCATCCAGCTTACACCTAGAAGATTTCACTCAAAA
+TGAACACTTTGAACTAAAGCTAGCCCAAACAATACCTAATTCAATTACCC
+TTAGTCACTTAACTAAAACATTCACCAAACCATTAAAGTATAGGAGATAG
+AAATTTTAACTTGGCGCTATAGAGAAAGTACCGTAAGGGAACGATGAAAG
+ATGCATTAAAAGTACTAAACAGCAAAGCTTACCCCTTTTACCTTTTGCAT
+AATGATTTAACTAGAATAAACTTAGCAAAGAGAACTTAAGCTAAGCACCC
+CGAAACCAGACGAGCTACCTATGAACAGTTACAAATGAACCAACTCATCT
+ATGTCGCAAAATAGTGAGAAGATTCGTAGGTAGAGGTGAAAAGCCCAACG
+AGCCTGGTGATAGCTGGTTGTCCAGAAACAGAATTTCAGTTCAAATTTAA
+ATTTACCTAAAAACTACTCAATTCTAATGTAAATTTAAATTATAGTCTAA
+AAAGGTACAGCTTTTTAGATACAGGTTACAACCTTCATTAGAGAGTAAGA
+ACAAGATAAACCCATAGTTGGCTTAAAAGCAGCCATCAATTAAGAAAGCG
+TTCAAGCTCAACGACACATCTATCTTAATCCCAACAATCAACCCAAACTA
+ACTCCTAATCTCATACTGGACTATTCTATCAACACATAGAAGCAATAATG
+TTAATATGAGTAACAAGAATTATTTCTCCTTGCATAAGCTTATATCAGAA
+CGAATACTCACTGATAGTTAACAACAAGATAGGGATAATCCAAAAACTAA
+TCATCTATTTAAACCATTGTTAACCCAACACAGGCATGCATCTATAAGGA
+AAGATTAAAAGAAGTAAAAGGAACTCGGCAAACACAAACCCCGCCTGTTT
+ACCAAAAACATCACCTCTAGCATTTCCAGTATTAGAGGCACTGCCTGCCC
+AGTGACATCTGTTtaaacggccgcggtatcctaaccgtgcaaaggtagca
+taatcacttgttccctaaatagggacttgtatgaatggccacacgagggt
+tttactgtctcttacttccaatcagtgaaattgaccttcccgtgaagagg
+cgggaatgactaaataagacgagaagaccctatggagcttTAATTAACTG
+ATTCACAAAAAACAACACACAAACCTTAACCTTCAGGGACAACAAAACTT
+TTGATTGAATCAGCAATTTCGGTTGGGGTGACCTCGGAGAACAAAACAAC
+CTCCGAGTGATTTAAATCCAGACTAACCAGTCAAAATATATAATCACTTA
+TTGATCCAAACCATTGATCAACGGAACAAGTTACCCTAGGGATAACAGCG
+CAATCCTATTCCAGAGTCCATATCGACAATTAGGGTTTACGACCTCGATG
+TTGGATCAAGACATCCTAATGGTGCAACCGCTATTAAGGGTTCGTTTGTT
+CAACGATTAAAGTCTTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGT
+CGGTTTCTATCTATTCTATACTTTTCCCAGTACGAAAGGACAAGAAAAGT
+AGGGCCCACTTTACAAGAAGCGCCCTCAAACTAATAGATGACATAATCTA
+AATCTAACTAATTTATAACTTCTACCGCCCTAGAACAGGGCTCgttaggg
+tggcagagcccggaaattgcataaaacttaaacctttacactcagaggtt
+caactcctctccctaacaacaTGTTCATAATTAACGTCCTCCTCCTAATT
+GTCCCAATCTTGCTCGCCGTAGCATTCCTCACACTAGTTGAACGAAAAGT
+CTTAGGCTATATGCAACTTCGCAAAGGACCCAACATCGTAGGCCCCTATG
+GCCTACTACAACCTATTGCCGATGCCCTCAAACTATTTATCAAAGAGCCA
+CTACAACCACTAACATCATCGACATCCATATTCATCATCGCACCAATCCT
+AGCCCTAACCCTGGCCTTAACCATATGAATCCCTCTGCCCATACCATACC
+CACTAATCAACATAAACCTAGGAATTCTATTCATACTAGCCATGTCCAGC
+CTAGCTGTCTACTCAATCCTTTGATCAGGATGGGCCTCAAACTCAAAATA
+CGCCCTAATTGGAGCTCTACGAGCAGTAGCACAAACCATCTCATACGAAG
+TAACTCTAGCAATCATCCTACTCTCAGTCCTCCTAATAAGCGGATCATTC
+ACATTATCAACACTTATTATTACCCAAGAATACCTCTGATTAATCTTCCC
+ATCATGACCCTTAGCCATAATGTGATTCATCTCAACATTAGCCGAAACCA
+ACCGAGCTCCATTTGACCTAACAGAAGGAGAATCAGAACTCGTCTCTGGA
+TTCAACGTTGAATACGCAGCCGGCCCATTTGCTCTATTCTTCCTAGCAGA
+ATACGCAAACATCATCATGATAAACATCTTCACAACAACCCTATTTCTAG
+GAGCATTTCACAACCCCTACCTGCCAGAACTCTACTCAATTAATTTCACC
+ATTAAAGCTCTCCTTCTAACATGTTCCTTCCTATGAATCCGAGCATCCTA
+CCCACGATTCCGATATGACCAACTTATACACCTCCTATGAAAGAACTTCC
+TACCACTCACACTAGCCCTCTGCATATGACACGTCTCACTTCCAATCATA
+CTATCCAGCATCCCACCACAAACATAGGAAATATGTCTGACAAAAGAGTT
+ACTTTGATAGAGTAAAACATAGAGGCTCAAACCCTCTTATTTctagaact
+acaggaattgaacctgctcctgagaattcaaaatcctccgtgctaccgaa
+ttacaccatgtcctaCAAGTAAGGTCAGCTAAATAAGCTATCGGGCCCAT
+ACCCCGAAAATGTTGGATTACACCCTTCCCGTACTAATAAATCCCCTTAT
+CTTCACAACTATTCTAATAACAGTTCTTCTAGGAACTATAATCGTTATAA
+TAAGCTCACACTGACTAATAATCTGAATCGGATTTGAAATAAATCTACTA
+GCCATTATCCCTATCCTAATAAAAAAGTACAATCCCCGAACCATAGAAGC
+CTCCACCAAATATTTTCTAACCCAAGCCACCGCATCAATACTCCTCATAA
+TAGCGATCATCATTAACCTCATACACTCAGGCCAATGAACAATCACAAAA
+GTCTTCAACCCCACAGCGTCCATCATTATAACTTCAGCTCTCGCCATAAA
+ACTTGGACTCACACCATTCCACTTCTGAGTACCCGAAGTCACACAGGGCA
+TCTCATTAACATCAGGTCTCATCCTACTTACATGACAAAAACTAGCCCCA
+ATATCAATCCTATATCAAATCTCACCCTCAATTAACCTAAATATCTTATT
+AACTATAGCCGTACTGTCAATCCTAGTAGGAGGCTGAGGCGGTCTCAACC
+AAACCCAACTACGAAAAATCATAGCATACTCGTCAATCGCGCATATAGGA
+TGAATAACAGCTGTCCTAGTATATAACCCAACACTAACAATACTAAACAT
+ATTAATTTACATTATAATAACACTCACAATATTCATACTATTTATCCACA
+GCTCCTCTACTACAACACTATCACTCTCCCACACATGAAACAAAATACCT
+CTAACCACTACACTAATCTTAATTACCTTACTATCCATAGGAGGCCTCCC
+CCCACTATCAGGATTCATACCCAAATGAATAATCATTCAAGAGCTCACCA
+AAAATAGCAGCATCATCCTCCCCACACTAATAGCCATTATAGCACTACTC
+AACCTCTACTTCTACATACGACTAACCTATTCCACCTCACTGACCATATT
+CCCATCCACAAACAACATAAAAATAAAATGACAATTCGAAACCAAACGAA
+TTACTCTCTTACCCCCGTTAATTGTTATATCCTCCCTACTCCTCCCCCTA
+ACCCCCATACTATCAATTTTGGACTAGGAATTTAGGTTAACATCCCAGAC
+CAAGAGCCTTCAAAGCTCTAAGCAAGTGAATCCACTTAATTCCTGCATAC
+TAAGGACTGCGAGACTCTATCTCACATCAATTGAACGCAAATCAAACTCT
+TTTATTAAGCTAAGCCCTTACTAGATTGGTGGGCTACCATCCCACGAAAT
+TTTAGTTAACAGCTAAATACCCTAATCAACTGGCTTCAATCTACTTCTCC
+CGCCGCCTAGAAAAAAAGGCGGGAGAAGCCCCGGCAGAAATTGAAGCTGC
+TCCTTTGAATTTGCAATTCAATGTGAAAATTCACCACGGGACTTGATAAG
+AAGAGGATTCCAACCCCTGTCTTTAGATTTACAGTCTAATGCTTACTCAG
+CCATCTTACCTATGTTCATCAACCGCTGACTATTTTCAACTAACCACAAA
+GACATCGGCACTCTGTACCTCCTATTCGGCGCTTGAGCTGGAATAGTAGG
+AACTGCCCTAAGCCTCCTAATCCGTGCTGAATTAGGCCAACCTGGGACCC
+TACTAGGAGATGATCAGATCTACAATGTCATTGTAACCGCCCATGCATTC
+GTAATAATTTTCTTTATGGTCATACCCATTATAATCGGAGGATTCGGAAA
+CTGATTAGTCCCCCTGATAATTGGAGCACCTGATATAGCTTTCCCCCGAA
+TAAACAACATAAGCTTCTGATTACTTCCCCCATCATTCCTACTTCTTCTC
+GCTTCCTCAATAATTGAAGCAGGTGCCGGAACAGGCTGAACCGTATATCC
+TCCTCTAGCTGGAAATCTGGCGCATGCAGGAGCCTCTGTTGACTTAACCA
+TTTTCTCTCTCCACCTAGCTGGGGTGTCCTCGATTTTAGGTGCCATCAAC
+TTTATTACCACAATCATTAACATAAAACCACCAGCCCTATCCCAATATCA
+AACCCCCCTATTCGTTTGATCTGTCCTTATTACGGCAGTACTCCTTCTCC
+TAGCCCTCCCGGTCCTAGCAGCAGGCATTACCATGCTTCTCACAGACCGT
+AACCTGAACACTACTTTCTTCGACCCCGCAGGAGGAGGGGATCCAATCCT
+TTATCAACACCTATTCTGATTCTTCGGACACCCCGAAGTCTATATTCTTA
+TCCTACCAGGCTTCGGTATAATCTCACACATCGTCACATACTACTCAGGT
+AAAAAGGAACCTTTTGGCTACATGGGTATAGTGTGAGCTATAATATCCAT
+TGGCTTTCTAGGCTTCATCGTATGGGCTCACCACATGTTTACAGTAGGGA
+TAGACGTTGACACACGAGCATACTTCACATCAGCTACCATAATCATCGCT
+ATCCCTACTGGTGTAAAAGTATTCAGCTGACTAGCCACCCTGCACGGAGG
+AAATATCAAATGATCTCCAGCTATACTCTGAGCTCTAGGCTTCATCTTCT
+TATTCACAGTAGGAGGTCTAACAGGAATCGTCCTAGCTAACTCATCCCTA
+GATATTGTTCTCCACGATACTTATTATGTAGTAGCACATTTCCATTATGT
+CCTGTCTATAGGAGCAGTCTTCGCCATTATGGGGGGATTTGTACACTGAT
+TCCCTCTATTCTCAGGATACACACTCAACCAAACCTGAGCAAAAATCCAC
+TTTACAATTATATTCGTAGGGGTAAATATAACCTTCTTCCCACAACATTT
+CCTTGGCCTCTCAGGAATGCCACGACGCTATTCTGATTATCCAGACGCAT
+ATACAACATGAAATACCATCTCATCCATAGGATCTTTTATCTCACTTACA
+GCAGTGATACTAATAATTTTCATAATTTGAGAAGCGTTCGCATCCAAACG
+AGAAGTGTCTACAGTAGAATTAACCTCAACTAATCTGGAATGACTACACG
+GATGCCCCCCACCATACCACACATTTGAAGAACCCACCTACGTAAACCTA
+AAAtaagaaaggaaggaatcgaaccccctctaactggtttcaagccaata
+tcataaccactatgtctttctcCATCAATTGAGGTATTAGTAAAAATTAC
+ATGACTTTGTCAAAGTTAAATTATAGGTTAAACCCCTATATACCTCTATG
+GCCTACCCCTTCCAACTAGGATTCCAAGACGCAACATCCCCTATTATAGA
+AGAACTCCTACACTTCCACGACCACACACTAATAATCGTATTCCTAATTA
+GCTCTCTAGTATTATATATTATCTCATCAATACTAACAACTAAATTAACC
+CATACCAGCACCATAGATGCTCAAGAAGTAGAGACAATTTGAACGATTTT
+ACCAGCCATCATCCTTATTCTAATCGCCCTCCCATCCCTACGAATTCTAT
+ATATAATAGATGAAATCAATAATCCGTCCCTCACAGTCAAAACAATAGGC
+CACCAATGATACTGAAGCTACGAGTATACCGATTACGAAGACTTGACCTT
+TGACTCCTACATGATCCCCACATCAGACCTAAAACCAGGAGAATTACGTC
+TTCTAGAAGTCGACAATCGAGTGGTTCTCCCCATAGAAATAACCATCCGA
+ATGCTAATTTCATCCGAAGACGTCCTACACTCATGAGCTGTGCCCTCCCT
+AGGCCTAAAAACAGACGCTATCCCTGGGCGCCTAAATCAGACAACTCTCG
+TGGCCTCTCGACCAGGACTTTACTACGGTCAATGCTCAGAGATCTGCGGA
+TCAAACCACAGCTTTATACCAATTGTCCTTGAACTAGTTCCACTGAAACA
+CTTCGAAGAATGATCTGCATCAATATTATAAAGTCACTAAGAAGCTATTA
+TAGCATTAACCTTTTAAGTTAAAGATTGAGGGTTCAACCCCCTCCCTAGT
+GATATGCCACAGTTGGATACATCAACATGATTTATTAATATCGTCTCAAT
+AATCCTAACTCTATTTATTGTATTTCAACTAAAAATCTCAAAGCACTCCT
+ATCCGACACACCCAGAAGTAAAGACAACCAAAATAACAAAACACTCTGCC
+CCTTGAGAATCAAAATGAACGAAAATCTATTCGCCTCTTTCGCTACCCCA
+ACAATAGTAGGCCTCCCTATTGTAATTCTGATCATCATATTTCCCAGCAT
+CCTATTCCCCTCACCCAACCGACTAATCAACAATCGCCTAATCTCAATTC
+AACAATGGCTAGTCCAACTTACATCAAAACAAATAATAGCTATCCATAAC
+AGCAAAGGACAAACCTGAACTCTTATACTCATATCACTGATCCTATTCAT
+TGGCTCAACAAACTTATTAGGCCTACTACCTCACTCATTTACACCAACAA
+CACAACTATCAATAAACCTAGGCATAGCTATTCCCCTATGGGCAGGGACA
+GTATTCATAGGCTTTCGTCACAAAACAAAAGCAGCCCTAGCCCACTTTCT
+ACCTCAAGGGACGCCCATTTTCCTCATCCCCATACTAGTAATTATCGAGA
+CTATCAGCCTATTTATTCAACCTGTAGCCCTAGCCGTGCGGCTAACCGCT
+AACATTACCGCCGGACACCTCCTAATACACCTCATCGGAGGGGCAACACT
+AGCCCTCATAAGCATCAGCCCCTCAACAGCCCTTATTACGTTTATCATCC
+TAATTCTACTAACTATCCTCGAATTCGCAGTAGCTATAATCCAAGCCTAC
+GTATTCACTCTCCTGGTAAGCCTTTACTTACACGACAACACCTAATGACC
+CACCAAACCCACGCTTACCACATAGTAAACCCCAGCCCATGACCACTTAC
+AGGAGCCCTATCAGCCCTCCTGATAACATCAGGACTAGCCATGTGATTTC
+ACTTTAACTCAACCTTACTTCTAGCTATAGGGCTATTAACTAACATCCTT
+ACCATATATCAATGATGACGAGACATCATCCGAGAAAGCACATTCCAAGG
+CCATCACACATCAATCGTTCAAAAGGGACTCCGATATGGCATAATCCTTT
+TTATTATCTCAGAAGTCTTCTTCTTCTCTGGCTTCTTCTGAGCCTTTTAC
+CACTCAAGCCTAGCCCCCACACCCGAACTAGGCGGCTGCTGACCACCCAC
+AGGTATCCACCCCTTAAACCCCCTAGAAGTCCCCTTACTCAACACCTCAG
+TGCTCCTAGCATCTGGAGTCTCTATCACCTGAGCCCACCATAGCCTAATA
+GAAGGAAACCGTAAAAATATGCTCCAAGGCCTATTCATCACAATTTCACT
+AGGCGTATACTTCACCCTTCTCCAAGCCTCAGAATACTATGAAGCCTCAT
+TTACTATTTCAGATGGAGTATACGGATCAACATTTTTCGTAGCAACAGGG
+TTCCACGGACTACACGTAATTATCGGATCTACCTTCCTCATTGTATGTTT
+CCTACGCCAACTAAAATTCCACTTTACATCCAGCCACCACTTCGGATTCG
+AAGCAGCCGCTTGATACTGACACTTCGTCGACGTAGTCTGACTATTCTTG
+TACGTCTCTATTTATTGATGAGGATCCTATTCTTTTAGTATTGACCAGTA
+CAATTGACTTCCAATCAATCAGCTTCGGTATAACCCGAAAAAGAATAATA
+AACCTCATACTGACACTCCTCACTAACACATTACTAGCCTCGCTACTCGT
+ACTCATCGCATTCTGACTACCACAACTAAACATCTATGCAGAAAAAACCA
+GCCCATATGAATGCGGATTTGACCCTATAGGGTCAGCACGCCTCCCCTTC
+TCAATAAAATTTTTCTTAGTGGCCATTACATTTCTGCTATTCGACTTAGA
+AATTGCCCTCCTATTACCCCTTCCATGAGCATCCCAAACAACTAACCTAA
+ACACTATACTTATCATAGCACTAGTCCTAATCTCTCTTCTAGCCATCAGC
+CTAGCCTACGAATGAACCCAAAAAGGACTAGAATGAACTGAGTATGGTAA
+TTAGTTTAAACCAAAACAAATGATTTCGACTCATTAAACTATGATTAACT
+TCATAATTACCAACATGTCACTAGTCCATATTAATATCTTCCTAGCATTC
+ACAGTATCCCTCGTAGGCCTACTAATGTACCGATCCCACCTAATATCCTC
+ACTCCTATGCCTAGAAGGAATAATACTATCACTATTCGTCATAGCAACCA
+TAATAGTCCTAAACACCCACTTCACACTAGCTAGTATAATACCTATCATC
+TTACTAGTATTTGCTGCCTGCGAACGAGCTCTAGGATTATCCCTACTAGT
+CATAGTCTCCAATACTTATGGAGTAGACCACGTACAAAACCTTAACCTCC
+TCCAATGCTAAAAATTATCATTCCCACAATCATACTTATGCCCCTTACAT
+GACTATCAAAAAAGAATATAATCTGAATCAACACTACAACCTATAGTCTA
+TTAATCAGCCTTATCAGCCTATCCCTCCTAAACCAACCTAGCAACAATAG
+CCTAAACTTCTCACTAATATTCTTCTCCGATCCCCTATCAGCCCCACTTC
+TGGTGTTGACAACATGACTACTGCCACTAATACTCATAGCCAGCCAACAC
+CATCTATCTAAGGAACCACTAATCCGAAAAAAACTCTACATCACCATGCT
+AACCATACTTCAAACTTTCCTAATCATGACTTTTACCGCCACAGAACTAA
+TCTCCTTCTACATCCTATTTGAAGCCACATTAGTTCCAACACTAATTATC
+ATCACCCGCTGAGGCAACCAAACAGAACGCCTGAACGCAGGCCTCTACTT
+CCTATTCTACACACTAATAGGTTCCCTCCCACTCTTAGTTGCACTAATCT
+CTATCCAAAACCTAACAGGCTCACTAAACTTCCTATTAATTCAATACTGA
+AACCAAGCACTACCCGACTCTTGATCCAATATTTTCCTATGACTAGCATG
+TATAATAGCATTCATAGTCAAAATACCGGTATATGGTCTTCACCTCTGAC
+TCCCAAAAGCCCATGTAGAAGCCCCAATTGCCGGATCCATAGTGCTAGCA
+GCCATTCTACTAAAACTAGGAGGCTACGGAATACTACGAATTACAACAAT
+ACTAAACCCCCAAACTAGCTTTATAGCCTACCCCTTCCTCATACTATCCC
+TGTGAGGAATAATCATAACTAGTTCCATCTGCTTGCGACAAACCGATCTA
+AAATCACTTATTGCATACTCCTCTGTCAGCCACATAGCCCTAGTAATCGT
+AGCCGTCCTCATCCAAACACCATGAAGTTATATAGGAGCTACAGCCCTAA
+TAATCGCTCACGGCCTTACATCATCAATACTATTCTGCCTGGCAAACTCA
+AATTACGAACGTACCCATAGCCGAACTATAATCCTAGCCCGCGGGCTTCA
+AACACTTCTTCCCCTTATAGCAGCCTGATGACTATTAGCCAGCCTAACCA
+ACCTGGCCCTCCCTCCCAGCATTAACCTAATTGGAGAGCTATTCGTAGTA
+ATATCATCATTCTCATGATCAAATATTACCATTATCCTAATAGGAGCCAA
+TATCACCATCACCGCCCTCTACTCCCTATACATACTAATCACAACACAAC
+GAGGGAAATACACACACCATATCAACAGCATTAAACCTTCATTTACACGA
+GAAAACGCACTCATGGCCCTCCACATGACTCCCCTACTACTCCTATCACT
+TAACCCTAAAATTATCCTAGGCTTTACGTACTGTAAATATAGTTTAACAA
+AAACACTAGATTGTGGATCTAGAAACAGAAACTTAATATTTCTTATTTAC
+CGAGAAAGTATGCAAGAACTGCTAATTCATGCCCCCATGTCCAACAAACA
+TGGCTCTCTCAAACTTTTAAAGGATAGGAGCTATCCGTTGGTCTTAGGAA
+CCAAAAAATTGGTGCAACTCCAAATAAAAGTAATCAACATGTTCTCCTCC
+CTCATACTAGTTTCACTATTAGTACTAACCCTCCCAATCATATTATCAAT
+CTTCAATACCTACAAAAACAGCACGTTCCCGCATCATGTAAAAAACACTA
+TCTCATATGCCTTCATTACTAGCCTAATTCCCACTATAATATTTATTCAC
+TCTGGACAAGAAACAATTATCTCAAACTGACACTGAATAACCATACAAAC
+CCTCAAACTATCCCTAAGCTTCAAACTAGATTACTTCTCAATAATTTTCG
+TACCAGTAGCCCTATTCGTAACATGATCTATTATGGAATTCTCCCTATGA
+TACATGCACTCAGATCCTTACATTACTCGATTTTTTAAATACTTACTTAC
+ATTCCTCATCACTATAATAATTCTAGTCACAGCTAACAACCTTTTCCAAC
+TGTTCATCGGATGGGAGGGAGTAGGCATCATGTCATTCTTACTAATCGGA
+TGATGATACGGCCGAACAGATGCCAACACCGCGGCCCTTCAAGCAATCCT
+TTATAACCGCATCGGGGATATCGGCTTCATCATGGCCATAGCCTGATTCC
+TATTCAACACCAACACATGAGACCTCCAACAAATCTTCATACTCGACCCC
+AACCTTACCAACCTCCCGCTCCTAGGCCTCCTCCTAGCCGCAACTGGCAA
+ATCCGCTCAATTTGGACTCCACCCATGACTTCCTTCAGCCATAGAGGGCC
+CTACACCAGTCTCAGCCCTACTCCACTCCAGCACAATAGTTGTAGCAGGC
+GTCTTCCTGCTAATCCGCTTCCATCCACTAATAGAAAACAACAAAACAAT
+CCAGTCACTTACCCTATGCCTAGGAGCCATCACCACACTATTCACAGCAA
+TCTGCGCACTCACTCAAAACGATATCAAAAAAATCATTGCTTTCTCCACC
+TCCAGCCAACTAGGCCTGATAATCGTAACCATCGGTATCAATCAACCCTA
+CCTAGCATTCCTCCACATTTGCACTCACGCATTCTTCAAAGCTATACTAT
+TTATATGTTCCGGATCCATTATCCACAGCCTAAATGACGAGCAAGATATC
+CGAAAAATAGGCGGACTATTTAATGCAATACCCTTCACCACCACATCTCT
+AATTATTGGCAGCCTTGCACTCACCGGAATTCCTTTCCTCACAGGCTTCT
+ACTCCAAAGACCTCATCATCGAAACCGCCAACACATCGTACACCAACGCC
+TGAGCCCTACTAATAACTCTCATTGCCACATCCCTCACAGCTGTCTACAG
+TACCCGAATCATCTTCTTTGCACTCCTAGGGCAACCCCGCTTCCTCCCTC
+TGACCTCAATCAACGAAAATAACCCCTTTCTAATTAACTCCATCAAACGC
+CTCTTAATTGGCAGCATTTTTGCCGGATTCTTCATCTCCAACAATATCTA
+CCCCACAACCGTCCCAGAAATAACCATACCTACTTACATAAAACTCACCG
+CCCTCGCAGTAACCATCCTAGGATTTACACTAGCCCTAGAACTAAGCTTG
+ATAACCCATAACTTAAAACTAGAACACTCCACCAACGTATTCAAATTCTC
+CAACCTCCTAGGATACTACCCAACAATTATACACCGACTCCCACCGCTCG
+CTAACCTATCAATAAGCCAAAAATCAGCATCACTTCTACTAGACTCAATC
+TGACTAGAAAACATCCTGCCAAAATCTATCTCCCAGTTCCAAATAAAAAC
+CTCGATCCTAATTTCCACCCAAAAAGGACAAATCAAATTATATTTCCTCT
+CATTCCTCATCACCCTTACCCTAAGCATACTACTTTTTAATCTCCACGAG
+TAACCTCTAAAATTACCAAGACCCCAACAAGCAACGATCAACCAGTCACA
+ATCACAACCCAAGCCCCATAACTATACAATGCAGCAGCCCCTATAATTTC
+CTCACTAAACGCCCCAGAATCTCCAGTATCATAAATAGCTCAAGCCCCCA
+CACCACTAAACTTAAACACTACCCCCACTTCCTCACTCTTCAGAACATAT
+AAAACCAACATAACCTCCATCAACAACCCTAAAAGAAATACCCCCATAAC
+AGTCGTATTAGACACCCATACCTCAGGATACTGCTCAGTAGCCATAGCCG
+TTGTATAACCAAAAACAACCAACATTCCTCCCAAATAAATCAAAAACACC
+ATCAACCCCAAAAAGGACCCTCCAAAATTCATAATAATACCACAACCTAC
+CCCTCCACTTACAATCAGCACTAAACCCCCATAAATAGGTGAAGGTTTTG
+AAGAAAACCCCACAAAACTAACAACAAAAATAACACTCAAAATAAACACA
+ATATATGTCATCATTATTCCCACGTGGAATCTAACCACGACCAATGACAT
+GAAAAATCATCGTTGTATTTCAACTATAAGAACACCAATGACAAACATCC
+GGAAATCTCACCCACTAATTAAAATCATCAATCACTCTTTTATTGACCTA
+CCAGCCCCCTCAAACATTTCATCATGATGAAACTTCGGCTCCCTCCTAGG
+AATCTGCCTAATCCTCCAAATCTTAACAGGCCTATTCCTAGCCATACACT
+ACACATCAGACACGACAACTGCCTTCTCATCCGTCACTCACATCTGCCGA
+GACGTTAACTACGGATGAATTATTCGCTACCTCCATGCCAACGGAGCATC
+AATATTTTTTATCTGCCTCTTCATTCACGTAGGACGCGGCCTCTACTACG
+GCTCTTACACATTCCTAGAGACATGAAACATTGGAATCATCCTACTTTTC
+ACAGTTATAGCTACAGCATTCATGGGCTATGTCCTACCATGAGGCCAAAT
+ATCCTTTTGAGGAGCAACAGTCATCACGAACCTCCTATCAGCAATTCCCT
+ACATCGGTACTACCCTCGTCGAGTGAATCTGAGGTGGATTCTCAGTAGAC
+AAAGCCACCCTTACCCGATTTTTTGCTTTCCACTTCATCCTACCCTTCAT
+CATCACAGCCCTGGTAGTCGTACATTTACTATTTCTTCACGAAACAGGAT
+CTAATAACCCCTCAGGAATCCCATCCGATATGGACAAAATCCCATTCCAC
+CCATATTATACAATTAAAGACATCCTAGGACTCCTCCTCCTGATCTTGCT
+CCTACTAACTCTAGTATTATTCTCCCCCGACCTCCTAGGAGACCCAGACA
+ACTACACCCCAGCTAACCCTCTCAGCACTCCCCCTCATATTAAACCAGAA
+TGGTACTTCCTGTTTGCCTACGCCATCCTACGCTCCATTCCCAACAAACT
+AGGCGGCGTATTAGCCCTAATCCTCTCCATCCTGATCCTAGCACTCATCC
+CCACCCTCCACATATCAAAACAACGAAGCATAATATTCCGGCCTCTCAGC
+CAATGCGTATTCTGACTCTTAGTGGCAGACTTACTGACACTAACATGAAT
+CGGCGGACAGCCAGTGGAACACCCATACGTAATTATCGGCCAACTGGCCT
+CAATCCTCTACTTCTCCCTAATTCTCATTTTTATACCACTCGCAAGCACC
+ATCGAAAACAATCTTCTAAAATGAAGAGTCCCTGTAGTATATCGCACATT
+ACCCTGGTCTTGTAAACCAGAAAAGGGGGAAAACGTTTCCTCCCAAGGAC
+TATCAAGGAAGAAGCTCTAGCTCCACCATCAACACCCAAAGCTGAAATTC
+TACTTAAACTATTCCTTGATTTCTTCCCCTAAACGACAACAATTTACCCT
+CATGTGCTATGTCAGTATCAGATTATACCCCCACATAACACCATACCCAC
+CTGACATGCAATATCTTATGAATGGCCTATGTACGTCGTGCATTAAATTG
+TCTGCCCCATGAATAATAAGCATGTACATAATATCATTTATCTTACATAA
+GTACATTATATTATTGATCGTGCATACCCCATCCAAGTCAAATCATTTCC
+AGTCAACACGCATATCACAGCCCATGTTCCACGAGCTTAATCACCAAGCC
+GCGGGAAATCAGCAACCCTCCCAACTACGTGTCCCAATCCTCGCTCCGGG
+CCCATCCAAACGTGGGGGTTTCTACAATGAAACTATACCTGGCATCTGGT
+TCTTTCTTCAGGGCCATTCCCACCCAACCTCGCCCATTCTTTCCCCTTAA
+ATAAGACATCTCGATGGACTAATGACTAATCAGCCCATGCTCACACATAA
+CTGTGATTTCATGCATTTGGTATCTTTTTATATTTGGGGATGCTATGACT
+CAGCTATGGCCGTCAAAGGCCTCGACGCAGTCAATTAAATTGAAGCTGGA
+CTTAAATTGAACGTTATTCCTCCGCATCAGCAACCATAAGGTGTTATTCA
+GTCCATGGTAGCGGGACATAGGAAACAAgtgcacctgtgcacctgtgcac
+ctgtgcacctgtgcacctgtgcacctgtgcacctgtgcacctgtgcacct
+gtgcacctgtgcacctgtgcacctgtgcacctgtgcacctgtgcacctgt
+gcacctgtgcacctgtgcacctgtgcacctgtgcacctgtgcacctgtgc
+acctgtgcacctgtgcacctgtgcacctgtgcacctgtgcacctgtgcac
+ctgtgcacctACCCGCGCAGTAAGCAAGTAATATAGCTTTCTTAATCAAA
+CCCCCCCTACCCCCCATTAAACTCCACATATGTACATTCAACACAATCTT
+GCCAAACCCCAAAAACAAGACTAAACAATGCACAATACTTCATGAAGCTT
+AACCCTCGCATGCCAACCATAATAACTCAACACACCTAACAATCTTAACA
+GAACTTTCCCCCCGCCATTAATACCAACATGCTACTTTAATCAATAAAAT
+TTCCATAGACAGGCATCCCCCTAGATCTAATTTTCTAAATCTGTCAACCC
+TTCTTCCCCC
diff -r 000000000000 -r cb3966035753 test-data/sam_pileup_in1.bam
Binary file test-data/sam_pileup_in1.bam has changed
diff -r 000000000000 -r cb3966035753 test-data/sam_pileup_out1.pileup
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/sam_pileup_out1.pileup Tue Aug 20 11:58:50 2013 -0400
@@ -0,0 +1,3286 @@
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diff -r 000000000000 -r cb3966035753 test-data/sam_pileup_out2.pileup
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/sam_pileup_out2.pileup Tue Aug 20 11:58:50 2013 -0400
@@ -0,0 +1,3286 @@
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+chrM 15790 C C 30 0 25 1 , I
+chrM 15791 T N 0 0 0 1 , -
+chrM 15792 C C 30 0 25 1 , I
+chrM 15793 G G 30 0 25 1 , I
+chrM 15794 C C 30 0 25 1 , H
+chrM 15795 T T 30 0 25 1 , 9
+chrM 15796 C C 30 0 25 1 , I
+chrM 15797 C C 30 0 25 1 , I
+chrM 15798 G G 30 0 25 1 , I
+chrM 15799 G G 30 0 25 1 , I
+chrM 15800 G G 30 0 25 1 , I
+chrM 15801 C C 30 0 25 1 , I
+chrM 15802 C C 30 0 25 1 , I
+chrM 15803 C C 30 0 25 1 , I
+chrM 15804 A A 30 0 25 1 , I
+chrM 15805 T T 30 0 25 1 , D
+chrM 15806 C C 30 0 25 1 , I
+chrM 15807 C C 30 0 25 1 , I
+chrM 15808 A A 30 0 25 1 , I
+chrM 15809 A A 30 0 25 1 , I
+chrM 15810 A A 30 0 25 1 , I
+chrM 15811 C C 30 0 25 1 , I
+chrM 15812 G G 30 0 25 1 , I
+chrM 15813 T T 30 0 25 1 , I
+chrM 15814 G N 0 0 0 1 n "
+chrM 15815 G N 0 0 0 1 n "
+chrM 15816 G G 30 0 25 1 , I
+chrM 15817 G G 30 0 25 1 , I
+chrM 15818 G G 30 0 25 1 , I
+chrM 15819 T T 30 0 25 1 , I
+chrM 15820 T T 30 0 25 1 , I
+chrM 15821 T T 30 0 25 1 , I
+chrM 15822 C C 30 0 25 1 ,$ E
+chrM 16613 G G 30 0 25 1 ^:. E
+chrM 16614 C C 30 0 25 1 . I
+chrM 16615 A A 30 0 25 1 . I
+chrM 16616 T T 30 0 25 1 . I
+chrM 16617 C C 30 0 25 1 . I
+chrM 16618 C C 30 0 25 1 . I
+chrM 16619 C C 30 0 25 1 . I
+chrM 16620 C N 0 0 0 1 N "
+chrM 16621 C N 0 0 0 1 N "
+chrM 16622 T T 30 0 25 1 . I
+chrM 16623 A A 30 0 25 1 . I
+chrM 16624 G G 30 0 25 1 . I
+chrM 16625 A A 30 0 25 1 . @
+chrM 16626 T T 30 0 25 1 . I
+chrM 16627 C C 30 0 25 1 . I
+chrM 16628 T T 30 0 25 1 . I
+chrM 16629 A A 30 0 25 1 . I
+chrM 16630 A N 0 0 0 1 . +
+chrM 16631 T T 30 0 25 1 . I
+chrM 16632 T T 30 0 25 1 . I
+chrM 16633 T T 30 0 25 1 . I
+chrM 16634 T T 30 0 25 1 . I
+chrM 16635 C C 30 0 25 1 . I
+chrM 16636 T T 30 0 25 1 . I
+chrM 16637 A A 30 0 25 1 . 8
+chrM 16638 A A 30 0 25 1 . H
+chrM 16639 A A 30 0 25 1 . 8
+chrM 16640 T T 30 0 25 1 . I
+chrM 16641 C C 30 0 25 1 . I
+chrM 16642 T T 30 0 25 1 . I
+chrM 16643 G G 30 0 25 1 . I
+chrM 16644 T T 30 0 25 1 . I
+chrM 16645 C C 30 0 25 1 . I
+chrM 16646 A A 30 0 25 1 . I
+chrM 16647 A A 30 0 25 1 . C
+chrM 16648 C C 30 0 25 1 .$ >
diff -r 000000000000 -r cb3966035753 test-data/sam_to_bam_in1.sam
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/sam_to_bam_in1.sam Tue Aug 20 11:58:50 2013 -0400
@@ -0,0 +1,13 @@
+@HD VN:1.0 SO:coordinate
+@SQ SN:chrM LN:100001
+@RG ID:rg1 SM:s1
+HWI-EAS91_1_30788AAXX:1:1:1513:715 16 chrM 9563 25 36M * 0 0 CTGACTACCACAACTAAACATCTATGCNNAAAAAAC I+-II?IDIIIIIIIIIIIIIIIIIII""IIIIIII NM:i:1 X1:i:1 MD:Z:7N0N27 RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1698:516 16 chrM 2735 25 36M * 0 0 TTTACACTCAGAGGTTCAACTCCTCTCNNTAACAAC I9IIIII5IIIIIIIIIIIIIIIIIII""IIIIIII NM:i:1 X1:i:1 MD:Z:7N0N27 RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1491:637 16 chrM 10864 25 36M * 0 0 TGTAGAAGCCCCAATTGCCGGATCCATNNTGCTAGC DBAIIIIIIIIIIIFIIIIIIIIIIII""IIIIIII NM:i:1 X1:i:1 MD:Z:7N0N27 RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1711:249 16 chrM 10617 25 36M * 0 0 ACCAAACAGAACGCCTGAACGCAGGCCNNTACTTCC IIIIIIIIIIIIIIIIIIIIIIIIIII""IIIIIII NM:i:1 X1:i:1 MD:Z:7N0N27 RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1634:211 0 chrM 9350 25 36M * 0 0 GAAGCAGNNGCTTGATACTGACACTTCGTCGACGTA IIIIIII""IIIIIIIIIIIIIIIIIIIIII9IIDF NM:i:1 X1:i:1 MD:Z:7N0N27 RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1218:141 16 chrM 14062 25 36M * 0 0 ACAAAACTAACAACAAAAATAACACTCNNAATAAAC I+IIII1IIIIIIIIIIIIIIIIIIII""IIIIIII NM:i:1 X1:i:1 MD:Z:7N0N27 RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1398:854 16 chrM 3921 25 36M * 0 0 CACCCTTCCCGTACTAATAAATCCCCTNNTCTTCAC IIIII=AIIIIIIIIIIIIIIBIIIII""IIIIIII NM:i:1 X1:i:1 MD:Z:7N0N27 RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1310:991 16 chrM 10002 25 36M * 0 0 CTCCTATGCCTAGAAGGAATAATACTANNACTATTC I:2IEI:IIDIIIIII4IIIIIIIIII""IIIIIII NM:i:1 X1:i:1 MD:Z:7N0N27 RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1716:413 0 chrM 6040 25 36M * 0 0 GATCCAANNCTTTATCAACACCTATTCTGATTCTTC IIIIIII""IIIIIIIIIIIIIIIIIIIIIIIIIII NM:i:1 X1:i:1 MD:Z:7N0N27 RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1630:59 16 chrM 12387 25 36M * 0 0 TCATACTCGACCCCAACCTTACCAACCNNCCGCTCC FIIHII;IIIIIIIIIIIIIIIIIIII""IIIIIII NM:i:1 X1:i:1 MD:Z:7N0N27 RG:Z:rg1
diff -r 000000000000 -r cb3966035753 test-data/sam_to_bam_out1.bam
Binary file test-data/sam_to_bam_out1.bam has changed
diff -r 000000000000 -r cb3966035753 test-data/sam_to_bam_out2.bam
Binary file test-data/sam_to_bam_out2.bam has changed
diff -r 000000000000 -r cb3966035753 tool-data/sam_fa_indices.loc.sample
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/tool-data/sam_fa_indices.loc.sample Tue Aug 20 11:58:50 2013 -0400
@@ -0,0 +1,28 @@
+#This is a sample file distributed with Galaxy that enables tools
+#to use a directory of Samtools indexed sequences data files. You will need
+#to create these data files and then create a sam_fa_indices.loc file
+#similar to this one (store it in this directory) that points to
+#the directories in which those files are stored. The sam_fa_indices.loc
+#file has this format (white space characters are TAB characters):
+#
+#index
+#
+#So, for example, if you had hg18 indexed stored in
+#/depot/data2/galaxy/sam/,
+#then the sam_fa_indices.loc entry would look like this:
+#
+#index hg18 /depot/data2/galaxy/sam/hg18.fa
+#
+#and your /depot/data2/galaxy/sam/ directory
+#would contain hg18.fa and hg18.fa.fai files:
+#
+#-rw-r--r-- 1 james universe 830134 2005-09-13 10:12 hg18.fa
+#-rw-r--r-- 1 james universe 527388 2005-09-13 10:12 hg18.fa.fai
+#
+#Your sam_fa_indices.loc file should include an entry per line for
+#each index set you have stored. The file in the path does actually
+#exist, but it should never be directly used. Instead, the name serves
+#as a prefix for the index file. For example:
+#
+#index hg18 /depot/data2/galaxy/sam/hg18.fa
+#index hg19 /depot/data2/galaxy/sam/hg19.fa
diff -r 000000000000 -r cb3966035753 tool-data/tool_data_table_conf.xml.sample
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/tool-data/tool_data_table_conf.xml.sample Tue Aug 20 11:58:50 2013 -0400
@@ -0,0 +1,8 @@
+
+
+
+
+ line_type, value, path
+
+
+
\ No newline at end of file
diff -r 000000000000 -r cb3966035753 tool_dependencies.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml Tue Aug 20 11:58:50 2013 -0400
@@ -0,0 +1,6 @@
+
+
+
+
+
+