Mercurial > repos > devteam > picard1106
changeset 61:b5063e189f57 draft
Deleted selected files
author | devteam |
---|---|
date | Tue, 18 Feb 2014 16:32:46 -0500 |
parents | 5de8c5774a10 |
children | 876ea92b5c28 |
files | picard_DownsampleSam.xml |
diffstat | 1 files changed, 0 insertions(+), 57 deletions(-) [+] |
line wrap: on
line diff
--- a/picard_DownsampleSam.xml Tue Feb 18 15:57:17 2014 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,57 +0,0 @@ -<tool name="Downsample SAM" id="picard_DownsampleSam" version="1.106.0"> - <!-- found on https://bitbucket.org/bwlang/galaxy-dist/src/ca5ded2e18a9ef802c31429e3cb861e8775b24d0/tools/picard/picard_DownsampleSam.xml --> - <requirements><requirement type="package" version="1.106.0">picard</requirement></requirements> - <command interpreter="python"> - picard_wrapper.py - --input=$inputFile - --output=$outFile - --probability=$probability - --seed=$seed - -j "\$JAVA_JAR_PATH/DownsampleSam.jar" - </command> - <inputs> - <param format="sam" name="inputFile" type="data" label="SAM dataset to be downsampled" - help="If empty, upload or import a SAM dataset." /> - <param name="probability" type="float" size="5" label="Probability (between 0 and 1) that any given read will be kept" help="specify 1 to keep all reads, 0.1 to keep 10% of the reads" value="1" /> - <param name="seed" type="integer" size="5" label="Random seed value" help="(same seed + same probability = same set of reads kept)" value="1" /> - - </inputs> - <outputs> - <data name="outFile" format="sam" label="${tool.name} on ${on_string}"> - </data> - </outputs> - <tests> - <test> - <param name="inputFile" value="cleansamtestinput.sam" /> - <param name="probability" value="0.1" /> - <param name="seed" value="2" /> - <output name="outFile" file="downsamplesamoutput.sam" ftype="sam" /> - </test> - </tests> - <help> - -.. class:: infomark - -**Purpose** - -Randomly down-sample a SAM or BAM file to retain a random subset of the reads. Mate-pairs are either both kept or both discarded. Reads marked as not primary alignments are all discarded. Each read is given a probability P of being retained - results with the exact same input in the same order and with the same value for RANDOM_SEED will produce the same results. - -**Picard documentation** - -This is a Galaxy wrapper for DownsampleSam, a part of the external package Picard-tools_. - - .. _Picard-tools: http://www.google.com/search?q=picard+samtools - ------- - -.. class:: warningmark - -**Warning on SAM/BAM quality** - -Many SAM/BAM files produced externally and uploaded to Galaxy do not fully conform to SAM/BAM specifications. Galaxy deals with this by using the **LENIENT** -flag when it runs Picard, which allows reads to be discarded if they're empty or don't map. This appears -to be the only way to deal with SAM/BAM that cannot be parsed. - - - </help> -</tool> \ No newline at end of file