# HG changeset patch # User devteam # Date 1393279200 18000 # Node ID 7aa4e1ac9d995baad2f68b1fa8ebc5f5c7883698 # Parent 55b6cd128a410bbc3390c5eaa593da0f54b97140 Uploaded diff -r 55b6cd128a41 -r 7aa4e1ac9d99 picard_FastqToSam.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/picard_FastqToSam.xml Mon Feb 24 17:00:00 2014 -0500 @@ -0,0 +1,154 @@ + + creates an unaligned BAM or SAM file + picard + + + fastq2sam_wrapper.sh + "${outputtype}" + "${output_bam}" + "${sample_name}" + "${read_group_name}" + FASTQ="${input_fastq1}" + #if str( $input_fastq2) != "None": + FASTQ2="${input_fastq2}" + #end if + QUALITY_FORMAT="${ dict( fastqsanger='Standard', fastqcssanger='Standard', fastqillumina='Illumina', fastqsolexa='Solexa' )[ $input_fastq1.ext ] }" ##Solexa, Illumina, Standard + #if $param_type.param_type_selector == "advanced": + #if str( $param_type.library_name ) != "": + LIBRARY_NAME="${param_type.library_name}" + #end if + #if str( $param_type.platform_unit ) != "": + PLATFORM_UNIT="${param_type.platform_unit}" + #end if + #if str( $param_type.platform ) != "": + PLATFORM="${param_type.platform}" + #end if + #if str( $param_type.sequencing_center ) != "": + SEQUENCING_CENTER="${param_type.sequencing_center}" + #end if + #if str( $param_type.predicted_insert_size ) != "": + PREDICTED_INSERT_SIZE="${param_type.predicted_insert_size}" + #end if + #if str( $param_type.description.value ) != "": + DESCRIPTION="${param_type.description}" + #end if + #if str( $param_type.run_date ) != "": + RUN_DATE="${param_type.run_date}" + #end if + #if str( $param_type.min_q ) != "": + MIN_Q="${param_type.min_q}" + #end if + #if str( $param_type.max_q ) != "": + MAX_Q="${param_type.max_q}" + #end if + SORT_ORDER="${param_type.sort_order}" + #else: + SORT_ORDER=coordinate ##unsorted, queryname, coordinate; always use coordinate + #end if + 2>&1 + || echo "Error running Picard FastqToSAM" >&2 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +**What it does** + +Picard: FastqToSam converts FASTQ files to unaligned BAM files. + +------ + +Please cite the website "http://picard.sourceforge.net". + +------ + + +**Input formats** + +FastqToSam accepts FASTQ input files (note: the Fastq-sanger file format does not work with this Picard tool). If using paired-end data, you should select two FASTQ files. + +------ + +**Outputs** + +The output is in BAM or in SAM format, see http://samtools.sourceforge.net for more details. + +------- + +**FastqToSam settings** + +This is list of FastqToSam options:: + + READ_GROUP_NAME=String Read group name Default value: A. This option can be set to 'null' to clear the default value. + SAMPLE_NAME=String Sample name to insert into the read group header Required. + LIBRARY_NAME=String The library name to place into the LB attribute in the read group header Default value: null. + PLATFORM_UNIT=String The platform unit (often run_barcode.lane) to insert into the read group header Default value: null. + PLATFORM=String The platform type (e.g. illumina, solid) to insert into the read group header Default value: null. + SEQUENCING_CENTER=String The sequencing center from which the data originated Default value: null. + PREDICTED_INSERT_SIZE=Integer Predicted median insert size, to insert into the read group header Default value: null. + DESCRIPTION=String Inserted into the read group header Default value: null. + +