Mercurial > repos > devteam > picard1106
diff picard_FilterSamReads.xml @ 113:069428662d9c draft
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author | devteam |
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date | Tue, 25 Feb 2014 01:11:18 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/picard_FilterSamReads.xml Tue Feb 25 01:11:18 2014 -0500 @@ -0,0 +1,83 @@ +<tool name="FilterSamReads" id="picard_FilterSamReads" version="1.106.0"> +<description>Produces a new SAM or BAM file by including or excluding aligned reads or a list of reads names supplied in the READ_LIST_FILE from the INPUT SAM or BAM file.</description> +<requirements><requirement type="package" version="1.106.0">picard</requirement></requirements> + <command interpreter="bash"> + generic_outformat_wrapper.sh FilterSamReads $output1 $outformat + FILTER="${conditionfilter.Filter}" + #if str( $READ_LIST_FILE ): + READ_LIST_FILE="${READ_LIST_FILE}" + #end if + #if str( $SORT_ORDER ): + SORT_ORDER="${SORT_ORDER}" + #end if + #if str( $WRITE_READS_FILES ): + WRITE_READS_FILES="${WRITE_READS_FILES}" + #end if + #if str( $OUTPUT ): + OUTPUT="${OUTPUT}" + #end if + + VALIDATION_STRINGENCY=LENIENT + QUIET=True + TMP_DIR="${__new_file_path__}" + </command> + + <stdio> + <exit_code range="0" level="warning" description="Tool finished correctly" /> + </stdio> + + <inputs> + <param format="bam,sam" name="input_file" type="data" label="SAM/BAM dataset that will be filterd" help="If empty, upload or import a SAM/BAM dataset."/> + <conditional name="conditionfilter"> + <param name="Filter" type="select" help="Required. Possible values: {includeAligned [OUTPUT SAM/BAM will contain aligned reads only. INPUT SAM/BAM must be in queryname SortOrder. (Note that *both* first and second of paired reads must be aligned to be included in the OUTPUT SAM or BAM)], excludeAligned [OUTPUT SAM/BAM will contain un-mapped reads only. INPUT SAM/BAM must be in queryname SortOrder. (Note that *both* first and second of pair must be aligned to be excluded from the OUTPUT SAM or BAM)], includeReadList [OUTPUT SAM/BAM will contain reads that are supplied in the READ_LIST_FILE file], excludeReadList [OUTPUT bam will contain reads that are *not* supplied in the READ_LIST_FILE file]}" + label="Filter"> + <option value="includeAligned" selected ="true">includeAligned</option> + <option value="excludeAligned">excludeAligned</option> + <option value="includeReadList">includeReadList</option> + <option value="excludeReadList">excludeReadList</option> + </param> + <when value="includeReadList"> + <param format="data" name="READ_LIST_FILE" type="data" label="Read List File containing reads that will be included or excluded from the OUTPUT SAM or BAM file. Default value: null." help="" /> + </when> + <when value="excludeReadList"> + <param format="data" name="READ_LIST_FILE" type="data" label="Read List File containing reads that will be included or excluded from the OUTPUT SAM or BAM file. Default value: null." help="" /> + </when> + </conditional> + + <param name="SORT_ORDER" value="Sort order" type="text" label="SortOrder of the OUTPUT SAM or BAM file, otherwise use the SortOrder of the INPUT file. Default value: null." help="Possible values: {unsorted, queryname, coordinate}" size="100" /> + + <param name="allowIncDictConcord" type="boolean" checked="False" truevalue="true" falsevalue="false" label="Allow incomplete dict concordance?" help="Allows a partial overlap of the BAM contigs with the new reference sequence contigs." /> + + <param name="WRITE_READS_FILES" type="boolean" checked="True" truevalue="true" falsevalue="false" label="Create .reads files (for debugging purposes)" help="" /> + + <param name="outputFormat" type="boolean" checked="True" truevalue="bam" falsevalue="sam" label="Output BAM instead of SAM" help="Uncheck for SAM output" /> + + </inputs> + + <outputs> + <data name="outFile" format="bam" label="${tool.name} on ${on_string}: filtered ${outputFormat}"> + <change_format> + <when input="outputFormat" value="sam" format="sam" /> + </change_format> + </data> + </outputs> + + <help> +Picard documentation says: + + +FilterSamReads + +Produces a new SAM or BAM file by including or excluding aligned reads or a list of reads names supplied in the READ_LIST_FILE from the INPUT SAM or BAM file. + +Option Description +INPUT=File The SAM or BAM file that will be filtered. Required. +FILTER=Filter Filter. Required. Possible values: {includeAligned [OUTPUT SAM/BAM will contain aligned reads only. INPUT SAM/BAM must be in queryname SortOrder. (Note that *both* first and second of paired reads must be aligned to be included in the OUTPUT SAM or BAM)], excludeAligned [OUTPUT SAM/BAM will contain un-mapped reads only. INPUT SAM/BAM must be in queryname SortOrder. (Note that *both* first and second of pair must be aligned to be excluded from the OUTPUT SAM or BAM)], includeReadList [OUTPUT SAM/BAM will contain reads that are supplied in the READ_LIST_FILE file], excludeReadList [OUTPUT bam will contain reads that are *not* supplied in the READ_LIST_FILE file]} +READ_LIST_FILE=File Read List File containing reads that will be included or excluded from the OUTPUT SAM or BAM file. Default value: null. +SORT_ORDER=SortOrder SortOrder of the OUTPUT SAM or BAM file, otherwise use the SortOrder of the INPUT file. Default value: null. Possible values: {unsorted, queryname, coordinate} +WRITE_READS_FILES=Boolean Create .reads files (for debugging purposes) Default value: true. This option can be set to 'null' to clear the default value. Possible values: {true, false} +OUTPUT=File SAM or BAM file to write read excluded results to Required. + + + </help> +</tool>