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1 <tool id="picard_mergesam" name="Merge SAM or BAM files" version="1.106.0">
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2 <!-- Documentation: http://picard.sourceforge.net/command-line-overview.shtml#MergeSamFiles
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3 Merges multiple SAM/BAM files into one file.
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4 -->
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5 <description>merges SAM or BAM files together</description>
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6 <requirements><requirement type="package" version="1.106.0">picard</requirement></requirements>
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7 <command interpreter="bash">
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8 mergesam_wrapper.sh $output1 $outformat I=$input1 I=$input2
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9 #for $i in $inputs
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10 I=${i.input}
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11 #end for
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12 MSD=$mergeSD VALIDATION_STRINGENCY=LENIENT TMP_DIR=$__new_file_path__
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13 2> /dev/null
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14 ##|| echo "Error running Picard MergeSamFiles" >&2
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15 </command>
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16 <inputs>
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17 <param name="title" label="Name for the output merged bam file" type="text" default="Merged.bam"
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18 help="This name will appear in your history so use it to remember what the new file in your history contains" />
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19 <param name="outformat" type="select" label="Output format" >
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20 <option value="bam" selected="True">BAM</option>
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21 <option value="sam">SAM</option>
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22 </param>
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23 <param name="mergeSD" value="true" type="boolean" label="Merge all component bam file headers into the merged bam file"
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24 truevalue="true" falsevalue="false" checked="yes"
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25 help="Control the MERGE_SEQUENCE_DICTIONARIES flag for Picard MergeSamFiles. Default (true) correctly propagates read groups and other important metadata" />
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26 <param name="input1" label="First file" type="data" format="bam,sam" />
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27 <param name="input2" label="with file" type="data" format="bam,sam" help="Need to add more files? Use controls below." />
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28 <repeat name="inputs" title="Input Files">
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29 <param name="input" label="Add file" type="data" format="bam,sam" />
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30 </repeat>
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31 </inputs>
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32 <outputs>
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33 <data format="bam" name="output1" label="${title}.${outformat}" >
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34 <change_format>
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35 <when input="outformat" value="sam" format="sam" />
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36 </change_format>
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37 </data>
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38 </outputs>
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39 <tests>
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40 <test>
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41 <!-- command: java -jar MergeSamFiles.jar O=mergesie.bam I=picard_output_fixmate_tiny.bam I=picard_RS_output1.bam MSD=True VALIDATION_STRINGENCY=LENIENT -->
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42 <param name="title" value="test1" />
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43 <param name="mergeSD" value="True" />
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44 <param name="input1" value="picard_output_fixmate_tiny.bam" ftype="bam" />
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45 <param name="input2" value="picard_RS_output1.bam" ftype="bam" />
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46 <output name="output1" file="mergesie.bam" ftype="bam" />
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47 </test>
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48 </tests>
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49 <help>
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50
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51 **What it does**
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52
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53 This tool uses the Picard_ merge command to merge any number of BAM files together into one BAM file while preserving the BAM
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54 metadata such as read groups
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55
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56 .. _Picard: http://picard.sourceforge.net/command-line-overview.shtml#MergeSamFiles
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57
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58 </help>
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59 </tool>
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