# HG changeset patch # User iuc # Date 1492124944 14400 # Node ID a459ead5ab75017a53104c71b3e7b4e03d18a838 # Parent 3d8d70436d02ec142205989b6dcef01cb5045a9d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/picard commit a55cff7dfc145ed17ec2ee9f6a70d51c6f9d74b6 diff -r 3d8d70436d02 -r a459ead5ab75 picard_CollectRnaSeqMetrics.xml --- a/picard_CollectRnaSeqMetrics.xml Wed Feb 08 12:41:25 2017 -0500 +++ b/picard_CollectRnaSeqMetrics.xml Thu Apr 13 19:09:04 2017 -0400 @@ -219,12 +219,12 @@ 9. A new dataset will appear in the current Galaxy history 10. Use this dataset as the input for **Gene annotations in refFlat form** dropdown of this tool -.. _refFlat: http://genome.ucsc.edu/goldenPath/gbdDescriptionsOld.html#RefFlat +.. _refFlat: https://genome.ucsc.edu/FAQ/FAQformat.html#format9 @description@ REF_FLAT=File Gene annotations in refFlat form. Format described here: - http://genome.ucsc.edu/goldenPath/gbdDescriptionsOld.html#RefFlat Required. + https://genome.ucsc.edu/FAQ/FAQformat.html#format9 Required. RIBOSOMAL_INTERVALS=File Location of rRNA sequences in genome, in interval_list format. If not specified no bases will be identified as being ribosomal. Format described here: diff -r 3d8d70436d02 -r a459ead5ab75 picard_MarkDuplicates.xml --- a/picard_MarkDuplicates.xml Wed Feb 08 12:41:25 2017 -0500 +++ b/picard_MarkDuplicates.xml Thu Apr 13 19:09:04 2017 -0400 @@ -1,4 +1,4 @@ - + examine aligned records in BAM datasets to locate duplicate molecules picard_macros.xml @@ -11,22 +11,28 @@ MarkDuplicates INPUT='$escaped_element_identifier' - OUTPUT="${outFile}" + OUTPUT='${outFile}' - METRICS_FILE="${metrics_file}" + METRICS_FILE='${metrics_file}' #for $element in $comments: - COMMENT="${element.comment}" + COMMENT='${element.comment}' #end for - REMOVE_DUPLICATES="${remove_duplicates}" - ASSUME_SORTED="${assume_sorted}" - DUPLICATE_SCORING_STRATEGY="${duplicate_scoring_strategy}" + REMOVE_DUPLICATES='${remove_duplicates}' + ASSUME_SORTED='${assume_sorted}' + + DUPLICATE_SCORING_STRATEGY='${duplicate_scoring_strategy}' #import pipes READ_NAME_REGEX=${ pipes.quote( str( $read_name_regex ) ) or "''" } - OPTICAL_DUPLICATE_PIXEL_DISTANCE="${optical_duplicate_pixel_distance}" + OPTICAL_DUPLICATE_PIXEL_DISTANCE='${optical_duplicate_pixel_distance}' - VALIDATION_STRINGENCY="${validation_stringency}" + # Optional arguments + #if $barcode_tag: + BARCODE_TAG='${barcode_tag}' + #end if + + VALIDATION_STRINGENCY='${validation_stringency}' QUIET=true VERBOSITY=ERROR @@ -53,6 +59,8 @@ + + @@ -116,6 +124,8 @@ unless using later versions of the Illumina pipeline that multiply pixel values by 10, in which case 50-100 is more normal. Default value: 100. + BARCODE_TAG=String Barcode SAM tag (ex. BC for 10X Genomics) Default value: null. + @more_info@