Mercurial > repos > devteam > picard
diff picard_CollectBaseDistributionByCycle.xml @ 3:52fdfc45590a draft
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
author | devteam |
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date | Thu, 16 Jul 2015 15:32:31 -0400 |
parents | |
children | 08f69add4d06 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/picard_CollectBaseDistributionByCycle.xml Thu Jul 16 15:32:31 2015 -0400 @@ -0,0 +1,114 @@ +<tool name="CollectBaseDistributionByCycle" id="picard_CollectBaseDistributionByCycle" version="@TOOL_VERSION@.0"> + <description>charts the nucleotide distribution per cycle in a SAM or BAM dataset</description> + <macros> + <import>picard_macros.xml</import> + </macros> + <expand macro="requirements"> + <requirement type="package" version="3.1.2">R</requirement> + </expand> + <command> + @java_options@ + ##set up input files + + #set $reference_fasta_filename = "localref.fa" + + #if str( $reference_source.reference_source_selector ) == "history": + ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" && + #else: + #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path ) + #end if + + java -jar \$JAVA_JAR_PATH/picard.jar + CollectBaseDistributionByCycle + INPUT="${inputFile}" + OUTPUT="${outFile}" + CHART_OUTPUT="${pdfFile}" + ALIGNED_READS_ONLY="${aligned_reads_only}" + PF_READS_ONLY="${pf_reads_only}" + REFERENCE_SEQUENCE="${reference_fasta_filename}" + ASSUME_SORTED="${assume_sorted}" + + VALIDATION_STRINGENCY="${validation_stringency}" + QUIET=true + VERBOSITY=ERROR + + </command> + <inputs> + <param format="sam,bam" name="inputFile" type="data" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset."/> + <conditional name="reference_source"> + <param name="reference_source_selector" type="select" label="Load reference genome from"> + <option value="cached">Local cache</option> + <option value="history">History</option> + </param> + <when value="cached"> + <param name="ref_file" type="select" label="Using reference genome" help="REFERENCE_SEQUENCE"> + <options from_data_table="all_fasta"> + </options> + <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> + </param> + </when> + <when value="history"> + <param name="ref_file" type="data" format="fasta" label="Use the folloing dataset as the reference sequence" help="REFERENCE_SEQUENCE; You can upload a FASTA sequence to the history and use it as reference" /> + </when> + </conditional> + <param name="aligned_reads_only" type="boolean" label="Calculate the base distribution over aligned reads only" checked="true" truevalue="true" falsevalue="false" help="ALIGNED_READS_ONLY"/> + <param name="pf_reads_only" type="boolean" label="Calculate the base distribution over PF (passing filtering) reads only" checked="true" truevalue="true" falsevalue="false" help="PF_READS_ONLY"/> + <param name="assume_sorted" type="boolean" label="Assume the input file is already sorted" checked="true" truevalue="true" falsevalue="false" help="ASSUME_SORTED"/> + + <expand macro="VS" /> + + </inputs> + + <outputs> + <data format="tabular" name="outFile"/> + <data format="pdf" name="pdfFile"/> + </outputs> + + <tests> + <test> + <param name="aligned_reads_only" value="true" /> + <param name="pf_reads_only" value="true" /> + <param name="assume_sorted" value="true" /> + <param name="reference_source_selector" value="history" /> + <param name="ref_file" value="picard_CollectBaseDistributionByCycle_ref.fa" /> + <param name="inputFile" value="picard_CollectBaseDistributionByCycle.bam" ftype="bam" /> + <output name="outFile" file="picard_CollectBaseDistributionByCycle_test1.tab" ftype="tabular" lines_diff="4"/> + </test> + </tests> + + <stdio> + <exit_code range="1:" level="fatal"/> + </stdio> + + <help> + +.. class:: infomark + +**Purpose** + +Program to chart the nucleotide distribution per cycle in a SAM or BAM file. + +@dataset_collections@ + +@description@ + + ALIGNED_READS_ONLY=Boolean If set to true, calculate the base distribution over aligned reads only. Default value: + false. This option can be set to 'null' to clear the default value. Possible values: + {true, false} + + PF_READS_ONLY=Boolean If set to true calculate the base distribution over PF reads only. Default value: false. + This option can be set to 'null' to clear the default value. Possible values: {true, + false} + + REFERENCE_SEQUENCE=File + R=File Reference sequence fasta Default value: null. + + ASSUME_SORTED=Boolean + AS=Boolean If true (default), then the sort order in the header file will be ignored. Default + +@more_info@ + + </help> +</tool> + +