comparison picard_FilterSamReads.xml @ 8:e417b1d6288d draft

planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit bf94a1505c131fb3f67c867b6e1d886780efa42e

7:08f69add4d06

    <import>picard_macros.xml</import>
  </macros>
  <expand macro="requirements" />
  <command detect_errors="exit_code"><![CDATA[
    @java_options@
    
    ##Sam Sorting is performed here because FilterSamReads requires input to be in query-sorted order
    
    picard
    SortSam
    INPUT="${inputFile}"
    OUTPUT=query_sorted_bam.bam
    SORT_ORDER=queryname
    VALIDATION_STRINGENCY=LENIENT
    QUIET=true
    VERBOSITY=ERROR
    
    &&
    
    picard
    FilterSamReads
    INPUT=query_sorted_bam.bam
    FILTER="${filter_type.filter}"
    
    #if ( str( $filter_type.filter ) == "includeReadList" or str( $filter_type.filter ) == "excludeReadList" ):
      READ_LIST_FILE="${filter_type.read_list_file}"
    #end if
    
    OUTPUT="${outFile}"
    SORT_ORDER=coordinate
    VALIDATION_STRINGENCY="${validation_stringency}"
    QUIET=true
    VERBOSITY=ERROR
  
  ]]></command>
  <inputs>
    <param name="inputFile" type="data" format="sam,bam" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset"/>
    <conditional name="filter_type">
      <param name="filter" type="select" label="Select filtering type" help="FILTER; see Help for deatiled info">

      </when>
      <when value="excludeReadList">
        <param name="read_list_file" type="data" format="tabular" label="Dataset containing read names that will be EXCLUDED in the output" help="READ_LIST_FILE"/>
      </when>
    </conditional>
    
    <expand macro="VS" />
    
  </inputs> 
  
  <outputs>
    <data format="bam" name="outFile" label="${tool.name} on ${on_string}: filtered BAM"/>
  </outputs>
  
  <tests>
    <test>
      <param name="inputFile" value="picard_FilterSamReads.bam" ftype="bam"/>
      <param name="filter" value="includeReadList"/>
      <param name="read_list_file" value="picard_FilterSamReads_read_list_file.tab" ftype="tabular"/>

      <param name="read_list_file" value="picard_FilterSamReads_read_list_file.tab" ftype="tabular"/>
      <param name="validation_stringency" value="LENIENT"/>
      <output name="outFile" file="picard_FilterSamReads_exclude_reads_test2.bam" ftype="bam" lines_diff="4"/>
    </test>
  </tests>
  
  
  <help>

**Purpose**

Computes a number of metrics that are useful for evaluating coverage and performance of whole genome sequencing experiments.

.. class:: warningmark

**Warning on using this tool on BWA-MEM output**

This tool will likely fail on BAM datasets generated by BWA MEM as it generates partial read alignemnts. 

@dataset_collections@

@description@

  FILTER=Filter                 Filter.  Required. Possible values:
                                includeAligned [OUTPUT SAM/BAM will contain aligned 
                                reads only. (Note that *both* first and 
                                second of paired reads must be aligned to be included
                                in the OUTPUT SAM or BAM)], 
                                
                                excludeAligned [OUTPUT SAM/BAM will contain un-mapped reads only.
                                (Note that *both* first and second of pair must be aligned to be 
                                excluded from the OUTPUT SAM or BAM)]
                                
                                includeReadList [OUTPUT SAM/BAM will contain reads 
                                that are supplied in the READ_LIST_FILE file]
                                
                                excludeReadList [OUTPUT bam will contain 
                                reads that are *not* supplied in the READ_LIST_FILE file]} 

  READ_LIST_FILE=File
  RLF=File                      Read List File containing reads that will be included or excluded from the OUTPUT SAM or 
                                BAM file.  Default value: null. 
  
@more_info@

  </help>
</tool>

8:e417b1d6288d

    <import>picard_macros.xml</import>
  </macros>
  <expand macro="requirements" />
  <command detect_errors="exit_code"><![CDATA[
    @java_options@
    @symlink_element_identifier@
    ##Sam Sorting is performed here because FilterSamReads requires input to be in query-sorted order

    picard
    SortSam
    INPUT='$inputFile.element_identifier'
    OUTPUT=query_sorted_bam.bam
    SORT_ORDER=queryname
    VALIDATION_STRINGENCY=LENIENT
    QUIET=true
    VERBOSITY=ERROR

    &&

    picard
    FilterSamReads
    INPUT=query_sorted_bam.bam
    FILTER="${filter_type.filter}"

    #if ( str( $filter_type.filter ) == "includeReadList" or str( $filter_type.filter ) == "excludeReadList" ):
      READ_LIST_FILE="${filter_type.read_list_file}"
    #end if

    OUTPUT="${outFile}"
    SORT_ORDER=coordinate
    VALIDATION_STRINGENCY="${validation_stringency}"
    QUIET=true
    VERBOSITY=ERROR

  ]]></command>
  <inputs>
    <param name="inputFile" type="data" format="sam,bam" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset"/>
    <conditional name="filter_type">
      <param name="filter" type="select" label="Select filtering type" help="FILTER; see Help for deatiled info">

      </when>
      <when value="excludeReadList">
        <param name="read_list_file" type="data" format="tabular" label="Dataset containing read names that will be EXCLUDED in the output" help="READ_LIST_FILE"/>
      </when>
    </conditional>

    <expand macro="VS" />

  </inputs>

  <outputs>
    <data format="bam" name="outFile" label="${tool.name} on ${on_string}: filtered BAM"/>
  </outputs>

  <tests>
    <test>
      <param name="inputFile" value="picard_FilterSamReads.bam" ftype="bam"/>
      <param name="filter" value="includeReadList"/>
      <param name="read_list_file" value="picard_FilterSamReads_read_list_file.tab" ftype="tabular"/>

      <param name="read_list_file" value="picard_FilterSamReads_read_list_file.tab" ftype="tabular"/>
      <param name="validation_stringency" value="LENIENT"/>
      <output name="outFile" file="picard_FilterSamReads_exclude_reads_test2.bam" ftype="bam" lines_diff="4"/>
    </test>
  </tests>


  <help>

**Purpose**

Computes a number of metrics that are useful for evaluating coverage and performance of whole genome sequencing experiments.

.. class:: warningmark

**Warning on using this tool on BWA-MEM output**

This tool will likely fail on BAM datasets generated by BWA MEM as it generates partial read alignemnts.

@dataset_collections@

@description@

  FILTER=Filter                 Filter.  Required. Possible values:
                                includeAligned [OUTPUT SAM/BAM will contain aligned
                                reads only. (Note that *both* first and
                                second of paired reads must be aligned to be included
                                in the OUTPUT SAM or BAM)],

                                excludeAligned [OUTPUT SAM/BAM will contain un-mapped reads only.
                                (Note that *both* first and second of pair must be aligned to be
                                excluded from the OUTPUT SAM or BAM)]

                                includeReadList [OUTPUT SAM/BAM will contain reads
                                that are supplied in the READ_LIST_FILE file]

                                excludeReadList [OUTPUT bam will contain
                                reads that are *not* supplied in the READ_LIST_FILE file]}

  READ_LIST_FILE=File
  RLF=File                      Read List File containing reads that will be included or excluded from the OUTPUT SAM or
                                BAM file.  Default value: null.

@more_info@

  </help>
</tool>