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planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit bf94a1505c131fb3f67c867b6e1d886780efa42e
author devteam
date Tue, 06 Dec 2016 10:04:26 -0500
parents 08f69add4d06
children 41b8d087a2d2
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1 <tool name="Downsample SAM/BAM" id="picard_DownsampleSam" version="@TOOL_VERSION@.0">
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2 <description>Downsample a file to retain a subset of the reads</description>
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3 <macros>
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4 <import>picard_macros.xml</import>
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5 </macros>
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6 <expand macro="requirements" />
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7 <command detect_errors="exit_code"><![CDATA[
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8 @java_options@
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9 @symlink_element_identifier@
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10 picard
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11 DownsampleSam
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12 INPUT='$inputFile.element_identifier'
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13 OUTPUT="${outFile}"
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14 PROBABILITY=${probability}
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15 RANDOM_SEED=${seed}
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16 QUIET=true
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17 VERBOSITY=ERROR
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18 VALIDATION_STRINGENCY=${validation_stringency}
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19 ]]></command>
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20 <inputs>
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21 <param format="sam,bam" name="inputFile" type="data" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM or BAM dataset" />
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22 <param name="probability" type="float" min="0.0" max="1.0" label="Probability (between 0 and 1) that any given read will be kept" help="PROBABILITY; specify 1 to keep all reads, 0.1 to keep 10% of the reads" value="1" />
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23 <param name="seed" type="integer" label="Random seed value" help="RANDOM_SEED; default=1" value="1" />
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25 <expand macro="VS" />
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27 </inputs>
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30 <outputs>
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31 <data name="outFile" format="bam" label="${tool.name} on ${on_string}: downsampled BAM"/>
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32 </outputs>
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34 <tests>
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35 <test>
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36 <param name="inputFile" value="picard_DownsampleSam.bam" ftype="bam" />
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37 <param name="probability" value="0.1" />
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38 <param name="seed" value="1024" />
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39 <param name="validation_stringency" value="LENIENT" />
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40 <output name="outFile" file="picard_DownsampleSam_test1.bam" ftype="bam"/>
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41 </test>
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42 </tests>
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43 <help>
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45 .. class:: infomark
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47 **Purpose**
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49 Randomly down-sample a SAM or BAM file to retain a random subset of the reads. Mate-pairs are either both kept or both discarded. Reads marked as not primary alignments are all discarded. Each read is given a probability P of being retained - results with the exact same input in the same order and with the same value for RANDOM_SEED will produce the same results.
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51 @dataset_collections@
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53 @description@
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55 INPUT=File
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56 I=File The input SAM or BAM file to downsample. Required.
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58 OUTPUT=File
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59 O=File The output, downsampled, SAM or BAM file to write. Required.
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61 RANDOM_SEED=Long
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62 R=Long Random seed to use if reproducibilty is desired. Setting to null will cause multiple
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63 invocations to produce different results.
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64
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65 PROBABILITY=Double
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66 P=Double The probability of keeping any individual read, between 0 and 1.
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67
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70 @more_info@
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71 </help>
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72 </tool>