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annotate picard_CollectAlignmentSummaryMetrics.xml @ 3:52fdfc45590a draft

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planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
author devteam
date Thu, 16 Jul 2015 15:32:31 -0400
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children 2589e6207cb4
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1 <tool name="Collect Alignment Summary Metrics" id="picard_CASM" version="@TOOL_VERSION@.0">
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2 <description>writes a file containing summary alignment metrics</description>
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3 <macros>
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4 <import>picard_macros.xml</import>
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5 </macros>
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6 <expand macro="requirements" />
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7 <command>
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8 @java_options@
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9 ##set up input files
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11 #set $reference_fasta_filename = "localref.fa"
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13 #if str( $reference_source.reference_source_selector ) == "history":
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14 ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" &amp;&amp;
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15 #else:
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16 #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path )
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17 #end if
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19 java -jar \$JAVA_JAR_PATH/picard.jar
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20 CollectAlignmentSummaryMetrics
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21 INPUT="${inputFile}"
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22 OUTPUT="${outFile}"
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23 MAX_INSERT_SIZE=${maxinsert}
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24 #for $sequence in $adapters:
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25 ADAPTER_SEQUENCE="${sequence.adapter}"
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26 #end for
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27 METRIC_ACCUMULATION_LEVEL="${metric_accumulation_level}"
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28 IS_BISULFITE_SEQUENCED="${bisulphite}"
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30 REFERENCE_SEQUENCE="${reference_fasta_filename}"
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32 ASSUME_SORTED="${assume_sorted}"
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34 VALIDATION_STRINGENCY="${validation_stringency}"
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35 QUIET=true
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36 VERBOSITY=ERROR
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37
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38 </command>
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39 <inputs>
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40 <param format="sam,bam" name="inputFile" type="data" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset."/>
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41 <conditional name="reference_source">
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42 <param name="reference_source_selector" type="select" label="Load reference genome from">
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43 <option value="cached">Local cache</option>
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44 <option value="history">History</option>
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45 </param>
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46 <when value="cached">
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47 <param name="ref_file" type="select" label="Using reference genome" help="REFERENCE_SEQUENCE">
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48 <options from_data_table="all_fasta">
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49 </options>
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50 <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
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51 </param>
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52 </when>
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53 <when value="history">
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54 <param name="ref_file" type="data" format="fasta" label="Use the following dataset as the reference sequence" help="REFERENCE_SEQUENCE; You can upload a FASTA sequence to the history and use it as reference" />
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55 </when>
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56 </conditional>
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57 <param name="metric_accumulation_level" type="select" label="The level(s) at which to accumulate metrics" multiple="true" help="METRIC_ACCUMULATION_LEVEL">
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58 <option value="ALL_READS" selected="True">All reads</option>
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59 <option value="SAMPLE">Sample</option>
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60 <option value="LIBRARY">Library</option>
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61 <option value="READ_GROUP">Read group</option>
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62 </param>
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63 <param name="assume_sorted" type="boolean" label="Assume the input file is already sorted" checked="true" truevalue="true" falsevalue="false" help="ASSUME_SORTED"/>
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64 <param name="bisulphite" type="boolean" label="Input file contains Bisulphite sequenced reads" checked="false" falsevalue="false" truevalue="true" help="IS_BISULFITE_SEQUENCED"/>
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65 <repeat name="adapters" title="Adapter" min="0" help="You can provide multiple adaptor sequences">
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66 <param name="adapter" type="text" size="50" label="Use this adaptor sequence" help="ADAPTER_SEQUENCE"/>
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67 </repeat>
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68 <param name="maxinsert" value="100000" type="integer" label="Larger paired end reads and inter-chromosomal pairs considered chimeric" size="20" help="MAX_INSERT_SIZE"/>
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69
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70 <expand macro="VS" />
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72 </inputs>
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73
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74 <outputs>
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75 <data format="tabular" name="outFile" label="${tool.name} on ${on_string}: Summary stats"/>
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76 </outputs>
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78 <stdio>
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79 <exit_code range="1:" level="fatal"/>
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80 </stdio>
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81
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83 <tests>
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84 <test>
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85 <param name="bisulphite" value="false" />
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86 <param name="sorted" value="true" />
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87 <param name="adaptors" value="" />
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88 <param name="maxinsert" value="100000" />
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89 <param name="reference_source_selector" value="history" />
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90 <param name="ref_file" value="picard_CASM_ref.fa" />
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91 <param name="inputFile" value="picard_CASM.bam" ftype="bam" />
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92 <output name="outFile" file="picard_CASM_test1.tab" ftype="tabular" lines_diff="4"/>
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93 </test>
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94 </tests>
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95
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96 <help>
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97
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98 .. class:: infomark
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99
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100 **Purpose**
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101
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102 Reads a SAM or BAM file and writes a file containing summary alignment metrics.
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103
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104 @dataset_collections@
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105
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106 @description@
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107
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108 MAX_INSERT_SIZE=Integer Paired end reads above this insert size will be considered chimeric along with
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109 inter-chromosomal pairs. Default value: 100000.
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110
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111 ADAPTER_SEQUENCE=String List of adapter sequences to use when processing the alignment metrics This option may
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112 be specified 0 or more times.
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113
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114 METRIC_ACCUMULATION_LEVEL=MetricAccumulationLevel
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115 LEVEL=MetricAccumulationLevel The level(s) at which to accumulate metrics. Possible values: {ALL_READS, SAMPLE,
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116 LIBRARY, READ_GROUP} This option may be specified 0 or more times.
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117
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118 IS_BISULFITE_SEQUENCED=Boolean
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119 BS=Boolean Whether the SAM or BAM file consists of bisulfite sequenced reads.
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120
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121
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122 REFERENCE_SEQUENCE=File
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123 R=File Reference sequence fasta Default value: null.
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124
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125 ASSUME_SORTED=Boolean
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126 AS=Boolean If true (default), then the sort order in the header file will be ignored.
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127
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128 @more_info@
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129
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130 </help>
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131 </tool>
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132
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133