view test-data/tblastn_four_human_vs_rhodopsin.html @ 55:673539b0f155 draft

"planemo upload for repository https://github.com/peterjc/galaxy_blast/tree/master/tools/ncbi_blast_plus commit 47e892eba5a0cbf0874979d079e9da45a108754c-dirty"
author peterjc
date Wed, 17 Mar 2021 19:17:18 +0000
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<HEAD><TITLE>BLAST Search Results</TITLE></HEAD>
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<b>TBLASTN 2.10.1+</b>


<b><a
href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=R
etrieve&list_uids=9254694&dopt=Citation">Reference</a>:</b>
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Sch&auml;ffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.



Database: User specified sequence set (Input: rhodopsin_nucs.fasta).
           6 sequences; 10,296 total letters



<b>Query=</b> sp|Q9BS26|ERP44_HUMAN Endoplasmic reticulum resident protein 44
OS=Homo sapiens GN=ERP44 PE=1 SV=1

Length=406


***** No hits found *****



Lambda      K        H        a         alpha
   0.347    0.182    0.684    0.522     1.92 

Gapped
Lambda      K        H        a         alpha    sigma
   0.299   0.0710    0.270     1.10     13.8     14.5 

Effective search space used: 1239330


<b>Query=</b> sp|Q9NSY1|BMP2K_HUMAN BMP-2-inducible protein kinase OS=Homo sapiens
GN=BMP2K PE=1 SV=2

Length=1161


***** No hits found *****



Lambda      K        H        a         alpha
   0.334    0.170    0.615    0.522     1.92 

Gapped
Lambda      K        H        a         alpha    sigma
   0.299   0.0710    0.270     1.10     13.8     14.5 

Effective search space used: 3667980


<b>Query=</b> sp|P06213|INSR_HUMAN Insulin receptor OS=Homo sapiens GN=INSR PE=1
SV=4

Length=1382


***** No hits found *****



Lambda      K        H        a         alpha
   0.346    0.180    0.700    0.522     1.92 

Gapped
Lambda      K        H        a         alpha    sigma
   0.299   0.0710    0.270     1.10     13.8     14.5 

Effective search space used: 4374000


<b>Query=</b> sp|P08100|OPSD_HUMAN Rhodopsin OS=Homo sapiens GN=RHO PE=1 SV=1

Length=348
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

gi|57163782|ref|NM_001009242.1| Felis catus rhodopsin (RHO), mRNA     <a href=#Subject_1>732</a>     0.0  
gi|18148870|dbj|AB062417.1| Synthetic construct Bos taurus gene f...  <a href=#Subject_5>711</a>     0.0  
gi|283855822|gb|GQ290312.1| Myotis ricketti voucher GQX10 rhodops...  <a href=#Subject_4>658</a>     0.0  
gi|2734705|gb|U59921.1|BBU59921 Bufo bufo rhodopsin mRNA, complet...  <a href=#Subject_2>646</a>     0.0  
gi|12583664|dbj|AB043817.1| Conger myriaster conf gene for fresh ...  <a href=#Subject_6>626</a>     0.0  
gi|283855845|gb|GQ290303.1| Cynopterus brachyotis voucher 2002043...  <a href=#Subject_3>229</a>     3e-67


><a name=Subject_1></a> gi|57163782|ref|NM_001009242.1| Felis catus rhodopsin (RHO), 
mRNA
Length=1047

 Score = 732 bits (1689),  Expect = 0.0, Method: Compositional matrix adjust.
 Identities = 336/348 (97%), Positives = 343/348 (99%), Gaps = 0/348 (0%)
 Frame = +1

Query  1     MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY  60
             MNGTEGPNFYVPFSN TGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY
Sbjct  1     MNGTEGPNFYVPFSNKTGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY  180

Query  61    VTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG  120
             VTVQHKKLRTPLNYILLNLAVADLFMV GGFT+TLYTSLHGYFVFGPTGCNLEGFFATLG
Sbjct  181   VTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLG  360

Query  121   GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIP  180
             GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPL GWSRYIP
Sbjct  361   GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIP  540

Query  181   EGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQES  240
             EG+QCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMI+IFFCYGQLVFTVKEAAAQQQES
Sbjct  541   EGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIVIFFCYGQLVFTVKEAAAQQQES  720

Query  241   ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAI  300
             ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMT+PAFFAKS++I
Sbjct  721   ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTLPAFFAKSSSI  900

Query  301   YNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA  348
             YNPVIYIMMNKQFRNCMLTT+CCGKNPLGDDEAS T SKTETSQVAPA
Sbjct  901   YNPVIYIMMNKQFRNCMLTTLCCGKNPLGDDEASTTGSKTETSQVAPA  1044


><a name=Subject_5></a> gi|18148870|dbj|AB062417.1| Synthetic construct Bos taurus gene 
for rhodopsin, complete cds
Length=1047

 Score = 711 bits (1640),  Expect = 0.0, Method: Compositional matrix adjust.
 Identities = 325/348 (93%), Positives = 337/348 (97%), Gaps = 0/348 (0%)
 Frame = +1

Query  1     MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY  60
             MNGTEGPNFYVPFSN TGVVRSPFE PQYYLAEPWQFSMLAAYMFLLI+LGFPINFLTLY
Sbjct  1     MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIMLGFPINFLTLY  180

Query  61    VTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG  120
             VTVQHKKLRTPLNYILLNLAVADLFMV GGFT+TLYTSLHGYFVFGPTGCNLEGFFATLG
Sbjct  181   VTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLG  360

Query  121   GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIP  180
             GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPL GWSRYIP
Sbjct  361   GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIP  540

Query  181   EGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQES  240
             EG+QCSCGIDYYT   E NNESFVIYMFVVHF IP+I+IFFCYGQLVFTVKEAAAQQQES
Sbjct  541   EGMQCSCGIDYYTPHEETNNESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQES  720

Query  241   ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAI  300
             ATTQKAEKEVTRMVIIMVIAFLICW+PYA VAFYIFTHQGS+FGPIFMTIPAFFAK++A+
Sbjct  721   ATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAV  900

Query  301   YNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA  348
             YNPVIYIMMNKQFRNCM+TT+CCGKNPLGDDEAS TVSKTETSQVAPA
Sbjct  901   YNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA  1044


><a name=Subject_4></a> gi|283855822|gb|GQ290312.1| Myotis ricketti voucher GQX10 rhodopsin 
(RHO) mRNA, partial cds
Length=983

 Score = 658 bits (1517),  Expect = 0.0, Method: Compositional matrix adjust.
 Identities = 310/326 (95%), Positives = 322/326 (99%), Gaps = 0/326 (0%)
 Frame = +1

Query  11   VPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT  70
            VPFSN TGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT
Sbjct  1    VPFSNKTGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT  180

Query  71   PLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVV  130
            PLNYILLNLAVA+LFMV GGFT+TLYTS+HGYFVFG TGCNLEGFFATLGGEIALWSLVV
Sbjct  181  PLNYILLNLAVANLFMVFGGFTTTLYTSMHGYFVFGATGCNLEGFFATLGGEIALWSLVV  360

Query  131  LAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIPEGLQCSCGID  190
            LAIERYVVVCKPMSNFRFGENHAIMG+AFTWVMALACAAPPLAGWSRYIPEG+QCSCGID
Sbjct  361  LAIERYVVVCKPMSNFRFGENHAIMGLAFTWVMALACAAPPLAGWSRYIPEGMQCSCGID  540

Query  191  YYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQESATTQKAEKEV  250
            YYTLKPEVNNESFVIYMFVVHFTIPMI+IFFCYGQLVFTVKEAAAQQQESATTQKAEKEV
Sbjct  541  YYTLKPEVNNESFVIYMFVVHFTIPMIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEV  720

Query  251  TRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMN  310
            TRMVIIMV+AFLICW+PYASVAFYIFTHQGSNFGP+FMTIPAFFAKS++IYNPVIYIMMN
Sbjct  721  TRMVIIMVVAFLICWLPYASVAFYIFTHQGSNFGPVFMTIPAFFAKSSSIYNPVIYIMMN  900

Query  311  KQFRNCMLTTICCGKNPLGDDEASAT  336
            KQFRNCMLTT+CCGKNPLGDDEAS T
Sbjct  901  KQFRNCMLTTLCCGKNPLGDDEASTT  978


><a name=Subject_2></a> gi|2734705|gb|U59921.1|BBU59921 Bufo bufo rhodopsin mRNA, complete 
cds
Length=1574

 Score = 646 bits (1489),  Expect = 0.0, Method: Compositional matrix adjust.
 Identities = 290/342 (85%), Positives = 320/342 (94%), Gaps = 1/342 (0%)
 Frame = +3

Query  1     MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY  60
             MNGTEGPNFY+P SN TGVVRSPFEYPQYYLAEPWQ+S+L AYMFLLI+LGFPINF+TLY
Sbjct  42    MNGTEGPNFYIPMSNKTGVVRSPFEYPQYYLAEPWQYSILCAYMFLLILLGFPINFMTLY  221

Query  61    VTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG  120
             VT+QHKKLRTPLNYILLNLA A+ FMVL GFT T+Y+S+ GYF+ G TGC +EGFFATLG
Sbjct  222   VTIQHKKLRTPLNYILLNLAFANHFMVLCGFTVTMYSSMNGYFILGATGCYVEGFFATLG  401

Query  121   GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIP  180
             GEIALWSLVVLAIERYVVVCKPMSNFRF ENHA+MGVAFTW+MAL+CA PPL GWSRYIP
Sbjct  402   GEIALWSLVVLAIERYVVVCKPMSNFRFSENHAVMGVAFTWIMALSCAVPPLLGWSRYIP  581

Query  181   EGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQES  240
             EG+QCSCG+DYYTLKPEVNNESFVIYMFVVHFTIP+IIIFFCYG+LV TVKEAAAQQQES
Sbjct  582   EGMQCSCGVDYYTLKPEVNNESFVIYMFVVHFTIPLIIIFFCYGRLVCTVKEAAAQQQES  761

Query  241   ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAI  300
             ATTQKAEKEVTRMVIIMV+ FLICWVPYASVAF+IF+ QGS FGPIFMT+PAFFAKS++I
Sbjct  762   ATTQKAEKEVTRMVIIMVVFFLICWVPYASVAFFIFSNQGSEFGPIFMTVPAFFAKSSSI  941

Query  301   YNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEA-SATVSKTE  341
             YNPVIYIM+NKQFRNCM+TT+CCGKNP G+D+A SA  SKTE
Sbjct  942   YNPVIYIMLNKQFRNCMITTLCCGKNPFGEDDASSAATSKTE  1067


><a name=Subject_6></a> gi|12583664|dbj|AB043817.1| Conger myriaster conf gene for fresh 
water form rod opsin, complete cds
Length=1344

 Score = 626 bits (1444),  Expect = 0.0, Method: Compositional matrix adjust.
 Identities = 281/342 (82%), Positives = 311/342 (91%), Gaps = 1/342 (0%)
 Frame = +2

Query  1     MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY  60
             MNGTEGPNFY+P SNATGVVRSPFEYPQYYLAEPW FS L+AYMF LI+ GFPINFLTLY
Sbjct  23    MNGTEGPNFYIPMSNATGVVRSPFEYPQYYLAEPWAFSALSAYMFFLIIAGFPINFLTLY  202

Query  61    VTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG  120
             VT++HKKLRTPLNYILLNLAVADLFMV GGFT+T+YTS+HGYFVFGPTGCN+EGFFATLG
Sbjct  203   VTIEHKKLRTPLNYILLNLAVADLFMVFGGFTTTMYTSMHGYFVFGPTGCNIEGFFATLG  382

Query  121   GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIP  180
             GEIALW LVVLAIER++VVCKP++NFRFGE HAIMGV  TW MALACA PPL GWSRYIP
Sbjct  383   GEIALWCLVVLAIERWMVVCKPVTNFRFGESHAIMGVMVTWTMALACALPPLFGWSRYIP  562

Query  181   EGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQES  240
             EGLQCSCGIDYYT  P +NNESFVIYMF  HF+IP+ +I FCYG+LV TVKEAAAQQQES
Sbjct  563   EGLQCSCGIDYYTRAPGINNESFVIYMFTCHFSIPLAVISFCYGRLVCTVKEAAAQQQES  742

Query  241   ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAI  300
              TTQ+AE+EVTRMV+IMVI+FL+CWVPYASVA YIFTHQGS FGPIFMTIP+FFAKS+A+
Sbjct  743   ETTQRAEREVTRMVVIMVISFLVCWVPYASVAWYIFTHQGSTFGPIFMTIPSFFAKSSAL  922

Query  301   YNPVIYIMMNKQFRNCMLTTICCGKNPL-GDDEASATVSKTE  341
             YNP+IYI MNKQFR CM+TT+CCGKNP   +D ASAT SKTE
Sbjct  923   YNPMIYICMNKQFRHCMITTLCCGKNPFEEEDGASATSSKTE  1048


><a name=Subject_3></a> gi|283855845|gb|GQ290303.1| Cynopterus brachyotis voucher 20020434 
rhodopsin (RHO) gene, exons 1 through 5 and partial cds
Length=4301

 Score = 151 bits (342),  Expect(2) = 1e-71, Method: Compositional matrix adjust.
 Identities = 69/74 (93%), Positives = 73/74 (99%), Gaps = 0/74 (0%)
 Frame = +3

Query  239   ESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSA  298
             ESATTQKAEKEVTRMVIIMVIAFLICW+PYA VAFYIFTHQGSNFGPIFMT+PAFFAKS+
Sbjct  3147  ESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSNFGPIFMTLPAFFAKSS  3326

Query  299   AIYNPVIYIMMNKQ  312
             +IYNPVIYIMMNKQ
Sbjct  3327  SIYNPVIYIMMNKQ  3368


 Score = 126 bits (284),  Expect(2) = 1e-71, Method: Compositional matrix adjust.
 Identities = 54/59 (92%), Positives = 57/59 (97%), Gaps = 0/59 (0%)
 Frame = +2

Query  177   RYIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAA  235
             RYIPEG+QCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMI+IFFCYGQLVFTVKE  +
Sbjct  2855  RYIPEGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIVIFFCYGQLVFTVKEVRS  3031


 Score = 229 bits (523),  Expect = 3e-67, Method: Compositional matrix adjust.
 Identities = 107/111 (96%), Positives = 109/111 (98%), Gaps = 0/111 (0%)
 Frame = +1

Query  11   VPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT  70
            VPFSN TGVVRSPFE+PQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT
Sbjct  1    VPFSNKTGVVRSPFEHPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT  180

Query  71   PLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLGG  121
            PLNYILLNLAVADLFMV GGFT+TLYTSLHGYFVFGPTGCNLEGFFATLGG
Sbjct  181  PLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGG  333


 Score = 122 bits (276),  Expect = 2e-32, Method: Compositional matrix adjust.
 Identities = 55/59 (93%), Positives = 56/59 (95%), Gaps = 0/59 (0%)
 Frame = +3

Query  119   LGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSR  177
             L GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMG+A TWVMALACAAPPL GWSR
Sbjct  1404  LAGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGLALTWVMALACAAPPLVGWSR  1580


 Score = 57.7 bits (125),  Expect = 4e-12, Method: Compositional matrix adjust.
 Identities = 23/26 (88%), Positives = 24/26 (92%), Gaps = 0/26 (0%)
 Frame = +1

Query  312   QFRNCMLTTICCGKNPLGDDEASATV  337
             QFRNCMLTT+CCGKNPLGDDEAS T 
Sbjct  4222  QFRNCMLTTLCCGKNPLGDDEASTTA  4299



Lambda      K        H        a         alpha
   0.351    0.182    0.707    0.522     1.92 

Gapped
Lambda      K        H        a         alpha    sigma
   0.299   0.0710    0.270     1.10     13.8     14.5 

Effective search space used: 1049670


  Database: User specified sequence set (Input: rhodopsin_nucs.fasta).
    Posted date:  Unknown
  Number of letters in database: 10,296
  Number of sequences in database:  6



Matrix: BLOSUM80
Gap Penalties: Existence: 10, Extension: 1
Neighboring words threshold: 14
Window for multiple hits: 25
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