diff tool-data/blastdb_p.loc.sample @ 1:5e9d5e536b79 draft

Uploaded v0.1.02 preview 2, clarify sample blastdb loc files, etc
author peterjc
date Tue, 03 Mar 2015 05:32:18 -0500
parents 432ea9614cc9
children
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--- a/tool-data/blastdb_p.loc.sample	Fri Jan 30 08:27:28 2015 -0500
+++ b/tool-data/blastdb_p.loc.sample	Tue Mar 03 05:32:18 2015 -0500
@@ -1,30 +1,44 @@
-#This is a sample file distributed with Galaxy that is used to define a
-#list of protein BLAST databases, using three columns tab separated
-#(longer whitespace are TAB characters):
+# This is a sample file distributed with Galaxy that is used to define a
+# list of protein BLAST databases, using three columns tab separated:
 #
-#<unique_id>	<database_caption>	<base_name_path>
+# <unique_id>{tab}<database_caption>{tab}<base_name_path>
+#
+# The captions typically contain spaces and might end with the build date.
+# It is important that the actual database name does not have a space in
+# it, and that there are only two tabs on each line.
 #
-#The captions typically contain spaces and might end with the build date.
-#It is important that the actual database name does not have a space in
-#it, and that there are only two tabs on each line.
+# You can download the NCBI provided protein databases like NR from here:
+# ftp://ftp.ncbi.nlm.nih.gov/blast/db/
 #
-#So, for example, if your database is NR and the path to your base name
-#is /data/blastdb/nr, then the blastdb_p.loc entry would look like this:
-#
-#nr{tab}NCBI NR (non redundant){tab}/data/blastdb/nr
+# For simplicity, many Galaxy servers are configured to offer just a live
+# version of each NCBI BLAST database (updated with the NCBI provided
+# Perl scripts or similar). In this case, we recommend using the case
+# sensistive base-name of the NCBI BLAST databases as the unique id.
+# Consistent naming is important for sharing workflows between Galaxy
+# servers.
 #
-#and your /data/blastdb directory would contain all of the files associated
-#with the database, /data/blastdb/nr.*.
+# For example, consider the NCBI "non-redundant" protein BLAST database
+# where you have downloaded and decompressed the files under /data/blastdb/
+# meaning at the command line BLAST+ would be run with something like
+# which would look at the files /data/blastdb/nr.p*:
 #
-#Your blastdb_p.loc file should include an entry per line for each "base name"
-#you have stored. For example:
+# $ blastp -db /data/blastdb/nr -query ...
+#
+# In this case use nr (lower case to match the NCBI file naming) as the
+# unique id in the first column of blastdb_p.loc, giving an entry like
+# this:
+#
+# nr{tab}NCBI non-redundant (nr){tab}/data/blastdb/nr
 #
-#nr_05Jun2010	NCBI NR (non redundant) 05 Jun 2010	/data/blastdb/05Jun2010/nr
-#nr_15Aug2010	NCBI NR (non redundant) 15 Aug 2010	/data/blastdb/15Aug2010/nr
-#...etc...
+# Alternatively, rather than a "live" mirror of the NCBI databases which
+# are updated automatically, for full reproducibility the Galaxy Team
+# recommend saving date-stamped copies of the databases. In this case
+# your blastdb_p.loc file should include an entry per line for each
+# version you have stored. For example:
 #
-#You can download the NCBI provided protein databases like NR from here:
-#ftp://ftp.ncbi.nlm.nih.gov/blast/db/
+# nr_05Jun2010{tab}NCBI NR (non redundant) 05 Jun 2010{tab}/data/blastdb/05Jun2010/nr
+# nr_15Aug2010{tab}NCBI NR (non redundant) 15 Aug 2010{tab}/data/blastdb/15Aug2010/nr
+# ...etc...
 #
-#See also blastdb.loc which is for any nucleotide BLAST database, and
-#blastdb_d.loc which is for any protein domains databases (like CDD).
+# See also blastdb.loc which is for any nucleotide BLAST database, and
+# blastdb_d.loc which is for any protein domains databases (like CDD).