annotate tools/ncbi_blast_plus/ncbi_macros.xml @ 17:fc571deae017 draft

planemo upload for repository https://github.com/peterjc/galaxy_blast/tree/master/tools/ncbi_blast_plus commit 9e989fb08bbd04732d94688d00e781d58949508f-dirty
author peterjc
date Mon, 03 Apr 2017 15:31:27 -0400
parents 4dcc9429d6d2
children c5f20ca77de2
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1 <macros>
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2 <token name="@WRAPPER_VERSION@">0.2.00</token>
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3 <xml name="parallelism">
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4 <!-- If job splitting is enabled, break up the query file into parts -->
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5 <parallelism method="multi" split_inputs="query" split_mode="to_size" split_size="1000" merge_outputs="output1" />
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6 </xml>
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e98f6bd363ba planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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7 <xml name="preamble">
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8 <requirements>
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9 <requirement type="package" version="2.5.0">blast</requirement>
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10 </requirements>
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e98f6bd363ba planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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11 <stdio>
e98f6bd363ba planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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12 <!-- Anything other than zero is an error -->
e98f6bd363ba planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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13 <exit_code range="1:" />
e98f6bd363ba planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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14 <!-- Might see negative return codes for Unix signals via Python subprocess -->
e98f6bd363ba planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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15 <exit_code range=":-1" />
e98f6bd363ba planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
peterjc
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16 <!-- In case the return code has not been set properly check stderr too -->
e98f6bd363ba planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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17 <regex match="Error:" />
e98f6bd363ba planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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18 <regex match="Exception:" />
e98f6bd363ba planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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19 </stdio>
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20 <version_command>@BINARY@ -version</version_command>
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21 </xml>
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22 <xml name="output_change_format">
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23 <change_format>
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24 <when input="output.out_format" value="0" format="txt"/>
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25 <when input="output.out_format" value="0 -html" format="html"/>
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26 <when input="output.out_format" value="2" format="txt"/>
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27 <when input="output.out_format" value="2 -html" format="html"/>
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28 <when input="output.out_format" value="4" format="txt"/>
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29 <when input="output.out_format" value="4 -html" format="html"/>
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30 <when input="output.out_format" value="5" format="blastxml"/>
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31 </change_format>
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32 </xml>
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33 <xml name="input_out_format">
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34 <conditional name="output">
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35 <param name="out_format" type="select" label="Output format">
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36 <option value="6">Tabular (standard 12 columns)</option>
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37 <option value="ext" selected="True">Tabular (extended 25 columns)</option>
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38 <option value="cols">Tabular (select which columns)</option>
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39 <option value="5">BLAST XML</option>
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40 <option value="0">Pairwise text</option>
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41 <option value="0 -html">Pairwise HTML</option>
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42 <option value="2">Query-anchored text</option>
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43 <option value="2 -html">Query-anchored HTML</option>
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44 <option value="4">Flat query-anchored text</option>
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45 <option value="4 -html">Flat query-anchored HTML</option>
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46 <!--
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47 <option value="-outfmt 11">BLAST archive format (ASN.1)</option>
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48 -->
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49 </param>
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50 <when value="6"/>
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51 <when value="ext"/>
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52 <when value="cols">
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53 <param name="std_cols" type="select" multiple="true" display="checkboxes" label="Standard columns">
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54 <option selected="true" value="qseqid">qseqid = Query Seq-id (ID of your sequence)</option>
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55 <option selected="true" value="sseqid">sseqid = Subject Seq-id (ID of the database hit)</option>
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56 <option selected="true" value="pident">pident = Percentage of identical matches</option>
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57 <option selected="true" value="length">length = Alignment length</option>
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58 <option selected="true" value="mismatch">mismatch = Number of mismatches</option>
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59 <option selected="true" value="gapopen">gapopen = Number of gap openings</option>
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60 <option selected="true" value="qstart">qstart = Start of alignment in query</option>
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61 <option selected="true" value="qend">qend = End of alignment in query</option>
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62 <option selected="true" value="sstart">sstart = Start of alignment in subject (database hit)</option>
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63 <option selected="true" value="send">send = End of alignment in subject (database hit)</option>
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64 <option selected="true" value="evalue">evalue = Expectation value (E-value)</option>
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65 <option selected="true" value="bitscore">bitscore = Bit score</option>
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66 </param>
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67 <param name="ext_cols" type="select" multiple="true" display="checkboxes" label="Extended columns">
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68 <option value="sallseqid">sallseqid = All subject Seq-id(s), separated by a ';'</option>
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69 <option value="score">score = Raw score</option>
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70 <option value="nident">nident = Number of identical matches</option>
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71 <option value="positive">positive = Number of positive-scoring matches</option>
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72 <option value="gaps">gaps = Total number of gaps</option>
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73 <option value="ppos">ppos = Percentage of positive-scoring matches</option>
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74 <option value="qframe">qframe = Query frame</option>
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75 <option value="sframe">sframe = Subject frame</option>
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76 <option value="qseq">qseq = Aligned part of query sequence</option>
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77 <option value="sseq">sseq = Aligned part of subject sequence</option>
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78 <option value="qlen">qlen = Query sequence length</option>
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79 <option value="slen">slen = Subject sequence length</option>
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80 <option value="salltitles">salltitles = All subject title(s), separated by a '&lt;&gt;'</option>
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81 </param>
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82 <param name="ids_cols" type="select" multiple="true" display="checkboxes" label="Other identifier columns">
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83 <option value="qgi">qgi = Query GI</option>
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84 <option value="qacc">qacc = Query accesion</option>
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85 <option value="qaccver">qaccver = Query accesion.version</option>
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86 <option value="sallseqid">sallseqid = All subject Seq-id(s), separated by a ';'</option>
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87 <option value="sgi">sgi = Subject GI</option>
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88 <option value="sallgi">sallgi = All subject GIs</option>
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89 <option value="sacc">sacc = Subject accession</option>
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90 <option value="saccver">saccver = Subject accession.version</option>
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91 <option value="sallacc">sallacc = All subject accessions</option>
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92 <option value="stitle">stitle = Subject Title</option>
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93 </param>
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94 <param name="misc_cols" type="select" multiple="true" display="checkboxes" label="Miscellaneous columns">
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95 <option value="sstrand">sstrand = Subject Strand</option>
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96 <!-- Is it really worth including 'frames' given have 'qframe' and 'sframe'? -->
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97 <option value="frames">frames = Query and subject frames separated by a '/'</option>
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98 <option value="btop">btop = Blast traceback operations (BTOP)</option>
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99 <option value="qcovs">qcovs = Query Coverage Per Subject</option>
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100 <option value="qcovhsp">qcovhsp = Query Coverage Per HSP</option>
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101 </param>
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102 <param name="tax_cols" type="select" multiple="true" display="checkboxes" label="Taxonomy columns">
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103 <option value="staxids">staxids = unique Subject Taxonomy ID(s), separated by a ';' (in numerical order)</option>
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104 <!-- TODO, how to handle the taxonomy data file dependency? If missing these give N/A -->
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105 <option value="sscinames">sscinames = unique Subject Scientific Name(s), separated by a ';'</option>
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106 <option value="scomnames">scomnames = unique Subject Common Name(s), separated by a ';'</option>
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107 <option value="sblastnames">sblastnames = unique Subject Blast Name(s), separated by a ';' (in alphabetical order)</option>
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108 <option value="sskingdoms">sskingdoms = unique Subject Super Kingdom(s), separated by a ';' (in alphabetical order)</option>
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109 </param>
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110 </when>
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111 <when value="5"/>
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112 <when value="0"/>
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113 <when value="0 -html"/>
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114 <when value="2"/>
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115 <when value="2 -html"/>
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peterjc
parents:
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116 <when value="4"/>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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117 <when value="4 -html"/>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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118 </conditional>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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119 </xml>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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120 <xml name="input_scoring_matrix">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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121 <param name="matrix" type="select" label="Scoring matrix">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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122 <option value="BLOSUM90">BLOSUM90</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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123 <option value="BLOSUM80">BLOSUM80</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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124 <option value="BLOSUM62" selected="true">BLOSUM62 (default)</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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125 <option value="BLOSUM50">BLOSUM50</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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126 <option value="BLOSUM45">BLOSUM45</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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127 <option value="PAM250">PAM250</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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128 <option value="PAM70">PAM70</option>
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peterjc
parents:
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129 <option value="PAM30">PAM30</option>
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130 </param>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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131 </xml>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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132 <xml name="input_query_gencode">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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133 <param name="query_gencode" type="select" label="Query genetic code">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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134 <!-- See http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi for details -->
12
d8f2c1f560ec Uploaded v0.1.08, can search multiple local databases, fixes a pipe problem in blastdbcmd, and minor internal changes.
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135 <option value="1" selected="True">1. Standard</option>
0
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parents:
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136 <option value="2">2. Vertebrate Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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137 <option value="3">3. Yeast Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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138 <option value="4">4. Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma/Spiroplasma Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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139 <option value="5">5. Invertebrate Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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140 <option value="6">6. Ciliate, Dasycladacean and Hexamita Nuclear Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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141 <option value="9">9. Echinoderm Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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142 <option value="10">10. Euplotid Nuclear</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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143 <option value="11">11. Bacteria and Archaea</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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144 <option value="12">12. Alternative Yeast Nuclear</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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145 <option value="13">13. Ascidian Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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146 <option value="14">14. Flatworm Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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147 <option value="15">15. Blepharisma Macronuclear</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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148 <option value="16">16. Chlorophycean Mitochondrial Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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149 <option value="21">21. Trematode Mitochondrial Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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150 <option value="22">22. Scenedesmus obliquus mitochondrial Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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151 <option value="23">23. Thraustochytrium Mitochondrial Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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152 <option value="24">24. Pterobranchia mitochondrial code</option>
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peterjc
parents:
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153 </param>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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154 </xml>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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155 <xml name="input_db_gencode">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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156 <param name="db_gencode" type="select" label="Database/subject genetic code">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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157 <!-- See http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi for details -->
12
d8f2c1f560ec Uploaded v0.1.08, can search multiple local databases, fixes a pipe problem in blastdbcmd, and minor internal changes.
peterjc
parents: 10
diff changeset
158 <option value="1" selected="True">1. Standard</option>
0
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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159 <option value="2">2. Vertebrate Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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160 <option value="3">3. Yeast Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
161 <option value="4">4. Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma/Spiroplasma Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
162 <option value="5">5. Invertebrate Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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163 <option value="6">6. Ciliate, Dasycladacean and Hexamita Nuclear Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
164 <option value="9">9. Echinoderm Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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165 <option value="10">10. Euplotid Nuclear</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
166 <option value="11">11. Bacteria and Archaea</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
167 <option value="12">12. Alternative Yeast Nuclear</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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168 <option value="13">13. Ascidian Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
169 <option value="14">14. Flatworm Mitochondrial</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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170 <option value="15">15. Blepharisma Macronuclear</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
171 <option value="16">16. Chlorophycean Mitochondrial Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
172 <option value="21">21. Trematode Mitochondrial Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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173 <option value="22">22. Scenedesmus obliquus mitochondrial Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
174 <option value="23">23. Thraustochytrium Mitochondrial Code</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
175 <option value="24">24. Pterobranchia mitochondrial code</option>
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peterjc
parents:
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176 </param>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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177 </xml>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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178 <xml name="input_conditional_nucleotide_db">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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179 <conditional name="db_opts">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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180 <param name="db_opts_selector" type="select" label="Subject database/sequences">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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181 <option value="db" selected="True">Locally installed BLAST database</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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182 <option value="histdb">BLAST database from your history</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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183 <option value="file">FASTA file from your history (see warning note below)</option>
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peterjc
parents:
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184 </param>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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185 <when value="db">
10
e6337ef07e9a v0.1.08 can search against multiple locally installed databases
peterjc
parents: 9
diff changeset
186 <param name="database" type="select" multiple="true" label="Nucleotide BLAST database">
0
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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187 <options from_data_table="blastdb" />
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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188 </param>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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189 <param name="histdb" type="hidden" value="" />
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
190 <param name="subject" type="hidden" value="" />
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peterjc
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191 </when>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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192 <when value="histdb">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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193 <param name="database" type="hidden" value="" />
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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194 <param name="histdb" type="data" format="blastdbn" label="Nucleotide BLAST database" />
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
195 <param name="subject" type="hidden" value="" />
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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196 </when>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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197 <when value="file">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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198 <param name="database" type="hidden" value="" />
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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199 <param name="histdb" type="hidden" value="" />
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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200 <param name="subject" type="data" format="fasta" label="Nucleotide FASTA file to use as database"/>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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201 </when>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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202 </conditional>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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203 </xml>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
204 <xml name="input_conditional_protein_db">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
205 <conditional name="db_opts">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
206 <param name="db_opts_selector" type="select" label="Subject database/sequences">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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207 <option value="db" selected="True">Locally installed BLAST database</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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208 <option value="histdb">BLAST database from your history</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
209 <option value="file">FASTA file from your history (see warning note below)</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
210 </param>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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211 <when value="db">
10
e6337ef07e9a v0.1.08 can search against multiple locally installed databases
peterjc
parents: 9
diff changeset
212 <param name="database" type="select" multiple="true" label="Protein BLAST database">
0
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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213 <options from_data_table="blastdb_p" />
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
214 </param>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
215 <param name="histdb" type="hidden" value="" />
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
216 <param name="subject" type="hidden" value="" />
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peterjc
parents:
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217 </when>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
218 <when value="histdb">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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219 <param name="database" type="hidden" value="" />
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
220 <param name="histdb" type="data" format="blastdbp" label="Protein BLAST database" />
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peterjc
parents:
diff changeset
221 <param name="subject" type="hidden" value="" />
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peterjc
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222 </when>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
223 <when value="file">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
224 <param name="database" type="hidden" value="" />
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peterjc
parents:
diff changeset
225 <param name="histdb" type="hidden" value="" />
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peterjc
parents:
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226 <param name="subject" type="data" format="fasta" label="Protein FASTA file to use as database"/>
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peterjc
parents:
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227 </when>
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peterjc
parents:
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228 </conditional>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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229 </xml>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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230 <xml name="input_conditional_pssm">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
231 <conditional name="db_opts">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
232 <param name="db_opts_selector" type="select" label="Protein domain database (PSSM)">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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233 <option value="db" selected="True">Locally installed BLAST protein domain database</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
234 <option value="histdb">BLAST protein domain database from your history</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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235 </param>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
236 <when value="db">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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237 <param name="database" type="select" label="Protein domain database">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
238 <options from_data_table="blastdb_d" />
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peterjc
parents:
diff changeset
239 </param>
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240 <param name="histdb" type="hidden" value="" />
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241 <param name="subject" type="hidden" value="" />
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242 </when>
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243 <when value="histdb">
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244 <param name="database" type="hidden" value="" />
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245 <param name="histdb" type="data" format="blastdbd" label="Protein domain database" />
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246 <param name="subject" type="hidden" value="" />
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247 </when>
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peterjc
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248 </conditional>
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249 </xml>
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peterjc
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250 <xml name="input_conditional_choose_db_type">
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peterjc
parents:
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251 <conditional name="db_opts">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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252 <param name="db_type" type="select" label="Type of BLAST database">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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parents:
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253 <option value="nucl" selected="True">Nucleotide</option>
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peterjc
parents:
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254 <option value="prot">Protein</option>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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255 </param>
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256 <when value="nucl">
10
e6337ef07e9a v0.1.08 can search against multiple locally installed databases
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diff changeset
257 <param name="database" type="select" multiple="true" label="Nucleotide BLAST database">
0
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258 <options from_data_table="blastdb" />
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259 </param>
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260 </when>
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261 <when value="prot">
10
e6337ef07e9a v0.1.08 can search against multiple locally installed databases
peterjc
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diff changeset
262 <param name="database" type="select" multiple="true" label="Protein BLAST database">
0
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peterjc
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263 <options from_data_table="blastdb_p" />
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264 </param>
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265 </when>
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266 </conditional>
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267 </xml>
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268 <xml name="input_parse_deflines">
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269 <param name="parse_deflines" type="boolean" label="Should the query and subject defline(s) be parsed?" truevalue="-parse_deflines" falsevalue="" checked="false" help="This affects the formatting of the query/subject ID strings"/>
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270 </xml>
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271 <xml name="input_filter_query_default_false">
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272 <param name="filter_query" type="boolean" label="Filter out low complexity regions (with SEG)" truevalue="-seg yes" falsevalue="-seg no" checked="false" />
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273 </xml>
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274 <xml name="input_filter_query_default_true">
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275 <param name="filter_query" type="boolean" label="Filter out low complexity regions (with SEG)" truevalue="-seg yes" falsevalue="-seg no" checked="true" />
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276 </xml>
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277 <xml name="input_max_hits">
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278 <param name="max_hits" type="integer" value="0" label="Maximum hits to show" help="Use zero for default limits">
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279 <validator type="in_range" min="0" />
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280 </param>
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281 </xml>
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282 <xml name="input_evalue">
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283 <param name="evalue_cutoff" type="float" size="15" value="0.001" label="Set expectation value cutoff" />
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284 </xml>
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285 <xml name="input_word_size">
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286 <param name="word_size" type="integer" value="0" label="Word size for wordfinder algorithm" help="Use zero for default, otherwise minimum 2.">
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peterjc
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287 <validator type="in_range" min="0" />
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288 </param>
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289 </xml>
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290 <xml name="input_strand">
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peterjc
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291 <param name="strand" type="select" label="Query strand(s) to search against database/subject">
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292 <option value="-strand both">Both</option>
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293 <option value="-strand plus">Plus (forward)</option>
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294 <option value="-strand minus">Minus (reverse complement)</option>
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295 </param>
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296 </xml>
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297 <xml name="input_qcov_hsp_perc">
12
d8f2c1f560ec Uploaded v0.1.08, can search multiple local databases, fixes a pipe problem in blastdbcmd, and minor internal changes.
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parents: 10
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298 <param name="qcov_hsp_perc" type="float" value="0" min="0" max="100" label="Minimum query coverage per hsp (percentage, 0 to 100)" help="See also the output column qcovhsp"/>
0
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299 </xml>
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peterjc
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300 <xml name="advanced_options">
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peterjc
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301 <conditional name="adv_opts">
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302 <param name="adv_opts_selector" type="select" label="Advanced Options">
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peterjc
parents:
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303 <option value="basic" selected="True">Hide Advanced Options</option>
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peterjc
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304 <option value="advanced">Show Advanced Options</option>
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305 </param>
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306 <when value="basic" />
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307 <when value="advanced">
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308 <yield />
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309 </when>
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310 </conditional>
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311 </xml>
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312 <xml name="advanced_optional_id_files">
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313 <conditional name="adv_optional_id_files_opts">
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314 <param name="adv_optional_id_files_opts_selector" type="select"
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315 label="Restrict search of database to a given set of ID's"
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316 help="This feature provides a means to exclude ID's from a BLAST database search. The expectation values in the BLAST results are based upon the sequences actually searched, and not on the underlying database. Note this cannot be used when comparing against a FASTA file.">
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317 <option value="none" selected="True">No restriction, search the entire database</option>
2
6119ddccd8a3 Uploaded v0.1.02 preview 3, disable tests using blastdb*.loc since not supported on the ToolShed (yet).
peterjc
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318 <option value="gilist">GI identifiers</option>
6119ddccd8a3 Uploaded v0.1.02 preview 3, disable tests using blastdb*.loc since not supported on the ToolShed (yet).
peterjc
parents: 0
diff changeset
319 <option value="negative_gilist">Negative GI identifiers</option>
6119ddccd8a3 Uploaded v0.1.02 preview 3, disable tests using blastdb*.loc since not supported on the ToolShed (yet).
peterjc
parents: 0
diff changeset
320 <option value="seqidlist">Sequence identifiers (SeqId's)</option>
0
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321 </param>
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322 <when value="none" />
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323 <when value="gilist">
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peterjc
parents:
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324 <param name="gilist" type="data" format="txt" label="Restrict search of database to GI's listed in this file"
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peterjc
parents:
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325 help="This option is only available for database searches."/>
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326 </when>
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peterjc
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327 <when value="negative_gilist">
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parents:
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328 <param name="negative_gilist" type="data" format="txt" label="Restrict search of database to everything except the GI's listed in this file"
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parents:
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329 help="This option is only available for database searches."/>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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330 </when>
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331 <when value="seqidlist">
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peterjc
parents:
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332 <param name="seqidlist" type="data" format="txt" label=" Restrict search of database to list of SeqId's"
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peterjc
parents:
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333 help="This option is only available for database searches."/>
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334 </when>
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335 </conditional>
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336 </xml>
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parents:
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337 <token name="@ADV_QCOV_HSP_PERC@">
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peterjc
parents:
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338 #if float(str($adv_opts.qcov_hsp_perc)) &gt; 0:
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
339 -qcov_hsp_perc $adv_opts.qcov_hsp_perc
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peterjc
parents:
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340 #end if
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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341 </token>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
342 <token name="@ADV_ID_LIST_FILTER@">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
343 #if $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'negative_gilist':
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
344 -negative_gilist $adv_opts.adv_optional_id_files_opts.negative_gilist
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peterjc
parents:
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345 #elif $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'gilist':
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peterjc
parents:
diff changeset
346 -gilist $adv_opts.adv_optional_id_files_opts.gilist
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peterjc
parents:
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347 #elif $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'seqidlist':
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
348 -seqidlist $adv_opts.adv_optional_id_files_opts.seqidlist
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peterjc
parents:
diff changeset
349 #end if
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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parents:
diff changeset
350 </token>
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peterjc
parents:
diff changeset
351 <token name="@THREADS@">-num_threads "\${GALAXY_SLOTS:-8}"</token>
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peterjc
parents:
diff changeset
352 <token name="@BLAST_DB_SUBJECT@">
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peterjc
parents:
diff changeset
353 #if $db_opts.db_opts_selector == "db":
10
e6337ef07e9a v0.1.08 can search against multiple locally installed databases
peterjc
parents: 9
diff changeset
354 -db "${' '.join(str( $db_opts.database.fields.path ).split( ',' ))}"
0
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
355 #elif $db_opts.db_opts_selector == "histdb":
5
188d2aca045b Uploaded v0.1.04, fix regression with BLAST database from history
peterjc
parents: 3
diff changeset
356 -db "${os.path.join($db_opts.histdb.extra_files_path,'blastdb')}"
0
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peterjc
parents:
diff changeset
357 #else:
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
358 -subject "$db_opts.subject"
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peterjc
parents:
diff changeset
359 #end if
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
360 </token>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
361 <token name="@BLAST_OUTPUT@">-out "$output1"
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
362 ##Set the extended list here so when we add things, saved workflows are not affected
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peterjc
parents:
diff changeset
363 #if str($output.out_format)=="ext":
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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364 -outfmt "6 std sallseqid score nident positive gaps ppos qframe sframe qseq sseq qlen slen salltitles"
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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365 #elif str($output.out_format)=="cols"
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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366 ##Pick your own columns. Galaxy gives us it comma separated, BLAST+ wants space separated:
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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367 ##TODO - Can we catch the user picking no columns and raise an error here?
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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368 #set cols = (str($output.std_cols)+","+str($output.ext_cols)+","+str($output.ids_cols)+","+str($output.misc_cols)+","+str($output.tax_cols)).replace("None", "").replace(",,", ",").replace(",", " ").strip()
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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369 -outfmt "6 $cols"
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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diff changeset
370 #else:
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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371 -outfmt $output.out_format
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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372 #end if
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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373 </token>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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374 <token name="@ADV_FILTER_QUERY@">$adv_opts.filter_query</token>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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375 <token name="@ADV_MAX_HITS@">
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peterjc
parents:
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376 ## Need int(str(...)) because $adv_opts.max_hits is an InputValueWrapper object not a string
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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377 ## Note -max_target_seqs used to simply override -num_descriptions and -num_alignments
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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378 ## but this was changed in BLAST+ 2.2.27 onwards to force their use (raised with NCBI)
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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379 #if (str($adv_opts.max_hits) and int(str($adv_opts.max_hits)) > 0):
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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380 #if str($output.out_format) in ["6", "ext", "cols", "5"]:
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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381 ## Most output formats use this, including tabular and XML:
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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diff changeset
382 -max_target_seqs $adv_opts.max_hits
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
383 #else
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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384 ## Text and HTML output formats 0-4 currently need this instead:
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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385 -num_descriptions $adv_opts.max_hits -num_alignments $adv_opts.max_hits
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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386 #end if
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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387 #end if
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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388 </token>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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389 <token name="@ADV_WORD_SIZE@">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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390 #if (str($adv_opts.word_size) and int(str($adv_opts.word_size)) > 0):
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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391 -word_size $adv_opts.word_size
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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392 #end if
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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393 $adv_opts.parse_deflines
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
394 </token>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
395 <!-- @ON_DB_SUBJECT@ is for use with @BLAST_DB_SUBJECT@ -->
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peterjc
parents:
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396 <token name="@ON_DB_SUBJECT@">#if str($db_opts.db_opts_selector)=='db'
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
397 ${db_opts.database}
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
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398 #elif str($db_opts.db_opts_selector)=='histdb'
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
399 ${db_opts.histdb.name}
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
400 #else
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
401 ${db_opts.subject.name}
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
402 #end if</token>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
403 <token name="@REFERENCES@">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
404 Peter J. A. Cock, John M. Chilton, Björn Grüning, James E. Johnson, Nicola Soranzo (2015).
6
856b73016ba1 v0.1.05 - Update citation information now GigaScience paper is out
peterjc
parents: 5
diff changeset
405 NCBI BLAST+ integrated into Galaxy. *GigaScience* 4:39
856b73016ba1 v0.1.05 - Update citation information now GigaScience paper is out
peterjc
parents: 5
diff changeset
406 http://dx.doi.org/10.1186/s13742-015-0080-7
0
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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parents:
diff changeset
407
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
408 Christiam Camacho et al. (2009).
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
409 BLAST+: architecture and applications.
6
856b73016ba1 v0.1.05 - Update citation information now GigaScience paper is out
peterjc
parents: 5
diff changeset
410 *BMC Bioinformatics* 15;10:421.
0
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
411 http://dx.doi.org/10.1186/1471-2105-10-421
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peterjc
parents:
diff changeset
412
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
413 This wrapper is available to install into other Galaxy Instances via the Galaxy
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
414 Tool Shed at http://toolshed.g2.bx.psu.edu/view/devteam/ncbi_blast_plus
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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415 </token>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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diff changeset
416 <xml name="blast_citations">
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
417 <citations>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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diff changeset
418 <citation type="doi">10.1186/1471-2105-10-421</citation>
6
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parents: 5
diff changeset
419 <citation type="doi">10.1186/s13742-015-0080-7</citation>
0
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420 </citations>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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421 </xml>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
422 <token name="@OUTPUT_FORMAT@">**Output format**
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
423
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
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diff changeset
424 Because Galaxy focuses on processing tabular data, the default output of this
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
425 tool is tabular. The standard BLAST+ tabular output contains 12 columns:
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
426
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
427 ====== ========= ============================================
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
428 Column NCBI name Description
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
429 ------ --------- --------------------------------------------
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
430 1 qseqid Query Seq-id (ID of your sequence)
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
431 2 sseqid Subject Seq-id (ID of the database hit)
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
432 3 pident Percentage of identical matches
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
433 4 length Alignment length
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
434 5 mismatch Number of mismatches
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
435 6 gapopen Number of gap openings
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
436 7 qstart Start of alignment in query
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
437 8 qend End of alignment in query
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
438 9 sstart Start of alignment in subject (database hit)
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
439 10 send End of alignment in subject (database hit)
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peterjc
parents:
diff changeset
440 11 evalue Expectation value (E-value)
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
441 12 bitscore Bit score
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
442 ====== ========= ============================================
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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parents:
diff changeset
443
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
444 The BLAST+ tools can optionally output additional columns of information,
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
445 but this takes longer to calculate. Many commonly used extra columns are
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
446 included by selecting the extended tabular output. The extra columns are
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
447 included *after* the standard 12 columns. This is so that you can write
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
448 workflow filtering steps that accept either the 12 or 25 column tabular
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
449 BLAST output. Galaxy now uses this extended 25 column output by default.
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peterjc
parents:
diff changeset
450
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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parents:
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451 ====== ============= ===========================================
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
452 Column NCBI name Description
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
453 ------ ------------- -------------------------------------------
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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parents:
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454 13 sallseqid All subject Seq-id(s), separated by a ';'
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peterjc
parents:
diff changeset
455 14 score Raw score
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
456 15 nident Number of identical matches
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
457 16 positive Number of positive-scoring matches
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
458 17 gaps Total number of gaps
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
459 18 ppos Percentage of positive-scoring matches
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peterjc
parents:
diff changeset
460 19 qframe Query frame
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peterjc
parents:
diff changeset
461 20 sframe Subject frame
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
462 21 qseq Aligned part of query sequence
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
463 22 sseq Aligned part of subject sequence
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
464 23 qlen Query sequence length
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
465 24 slen Subject sequence length
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
466 25 salltitles All subject title(s), separated by a '&lt;&gt;'
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peterjc
parents:
diff changeset
467 ====== ============= ===========================================
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diff changeset
468
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
469 The third option is to customise the tabular output by selecting which
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
470 columns you want, from the standard set of 12, the default set of 25,
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
471 or any of the additional columns BLAST+ offers (including species name).
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
472
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
473 The fourth option is BLAST XML output, which is designed to be parsed by
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
474 another program, and is understood by some Galaxy tools.
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
475
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
476 You can also choose several plain text or HTML output formats which are designed to be read by a person (not by another program).
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
477 The HTML versions use basic webpage formatting and can include links to the hits on the NCBI website.
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
478 The pairwise output (the default on the NCBI BLAST website) shows each match as a pairwise alignment with the query.
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
479 The two query anchored outputs show a multiple sequence alignment between the query and all the matches,
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
480 and differ in how insertions are shown (marked as insertions or with gap characters added to the other sequences).
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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parents:
diff changeset
481 </token>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
482 <token name="@FASTA_WARNING@">.. class:: warningmark
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
483
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
484 You can also search against a FASTA file of subject (target)
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
485 sequences. This is *not* advised because it is slower (only one
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
486 CPU is used), but more importantly gives e-values for pairwise
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
487 searches (very small e-values which will look overly signficiant).
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
488 In most cases you should instead turn the other FASTA file into a
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
489 database first using *makeblastdb* and search against that.
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
peterjc
parents:
diff changeset
490 </token>
432ea9614cc9 Uploaded v0.1.02 preview 1, using tool_data_table_conf.xml for loc files, etc
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491 <token name="@SEARCH_TIME_WARNING@">.. class:: warningmark
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492
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493 **Note**. Database searches may take a substantial amount of time.
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494 For large input datasets it is advisable to allow overnight processing.
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495
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496 -----
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497 </token>
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498 </macros>