Mercurial > repos > devteam > ncbi_blast_plus
annotate tools/ncbi_blast_plus/ncbi_macros.xml @ 17:fc571deae017 draft
planemo upload for repository https://github.com/peterjc/galaxy_blast/tree/master/tools/ncbi_blast_plus commit 9e989fb08bbd04732d94688d00e781d58949508f-dirty
author | peterjc |
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date | Mon, 03 Apr 2017 15:31:27 -0400 |
parents | 4dcc9429d6d2 |
children | c5f20ca77de2 |
rev | line source |
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1 <macros> |
13 | 2 <token name="@WRAPPER_VERSION@">0.2.00</token> |
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3 <xml name="parallelism"> |
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4 <!-- If job splitting is enabled, break up the query file into parts --> |
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5 <parallelism method="multi" split_inputs="query" split_mode="to_size" split_size="1000" merge_outputs="output1" /> |
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6 </xml> |
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e98f6bd363ba
planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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7 <xml name="preamble"> |
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8 <requirements> |
13 | 9 <requirement type="package" version="2.5.0">blast</requirement> |
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10 </requirements> |
3
e98f6bd363ba
planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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11 <stdio> |
e98f6bd363ba
planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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12 <!-- Anything other than zero is an error --> |
e98f6bd363ba
planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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13 <exit_code range="1:" /> |
e98f6bd363ba
planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
peterjc
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14 <!-- Might see negative return codes for Unix signals via Python subprocess --> |
e98f6bd363ba
planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
peterjc
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15 <exit_code range=":-1" /> |
e98f6bd363ba
planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
peterjc
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16 <!-- In case the return code has not been set properly check stderr too --> |
e98f6bd363ba
planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
peterjc
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17 <regex match="Error:" /> |
e98f6bd363ba
planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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18 <regex match="Exception:" /> |
e98f6bd363ba
planemo upload for repository https://github.com/peterjc/galaxy_blast/tools/ncbi_blast_plus commit a567843bb239ee8faec72eb7e52f3b5aa3191631
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19 </stdio> |
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20 <version_command>@BINARY@ -version</version_command> |
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21 </xml> |
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22 <xml name="output_change_format"> |
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23 <change_format> |
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24 <when input="output.out_format" value="0" format="txt"/> |
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25 <when input="output.out_format" value="0 -html" format="html"/> |
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26 <when input="output.out_format" value="2" format="txt"/> |
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27 <when input="output.out_format" value="2 -html" format="html"/> |
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28 <when input="output.out_format" value="4" format="txt"/> |
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29 <when input="output.out_format" value="4 -html" format="html"/> |
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30 <when input="output.out_format" value="5" format="blastxml"/> |
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31 </change_format> |
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32 </xml> |
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33 <xml name="input_out_format"> |
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34 <conditional name="output"> |
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35 <param name="out_format" type="select" label="Output format"> |
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36 <option value="6">Tabular (standard 12 columns)</option> |
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37 <option value="ext" selected="True">Tabular (extended 25 columns)</option> |
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38 <option value="cols">Tabular (select which columns)</option> |
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39 <option value="5">BLAST XML</option> |
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40 <option value="0">Pairwise text</option> |
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41 <option value="0 -html">Pairwise HTML</option> |
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42 <option value="2">Query-anchored text</option> |
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43 <option value="2 -html">Query-anchored HTML</option> |
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44 <option value="4">Flat query-anchored text</option> |
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45 <option value="4 -html">Flat query-anchored HTML</option> |
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46 <!-- |
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47 <option value="-outfmt 11">BLAST archive format (ASN.1)</option> |
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48 --> |
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49 </param> |
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50 <when value="6"/> |
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51 <when value="ext"/> |
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52 <when value="cols"> |
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53 <param name="std_cols" type="select" multiple="true" display="checkboxes" label="Standard columns"> |
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54 <option selected="true" value="qseqid">qseqid = Query Seq-id (ID of your sequence)</option> |
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55 <option selected="true" value="sseqid">sseqid = Subject Seq-id (ID of the database hit)</option> |
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56 <option selected="true" value="pident">pident = Percentage of identical matches</option> |
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57 <option selected="true" value="length">length = Alignment length</option> |
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58 <option selected="true" value="mismatch">mismatch = Number of mismatches</option> |
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59 <option selected="true" value="gapopen">gapopen = Number of gap openings</option> |
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60 <option selected="true" value="qstart">qstart = Start of alignment in query</option> |
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61 <option selected="true" value="qend">qend = End of alignment in query</option> |
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62 <option selected="true" value="sstart">sstart = Start of alignment in subject (database hit)</option> |
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63 <option selected="true" value="send">send = End of alignment in subject (database hit)</option> |
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64 <option selected="true" value="evalue">evalue = Expectation value (E-value)</option> |
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65 <option selected="true" value="bitscore">bitscore = Bit score</option> |
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66 </param> |
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67 <param name="ext_cols" type="select" multiple="true" display="checkboxes" label="Extended columns"> |
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68 <option value="sallseqid">sallseqid = All subject Seq-id(s), separated by a ';'</option> |
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69 <option value="score">score = Raw score</option> |
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70 <option value="nident">nident = Number of identical matches</option> |
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71 <option value="positive">positive = Number of positive-scoring matches</option> |
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72 <option value="gaps">gaps = Total number of gaps</option> |
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73 <option value="ppos">ppos = Percentage of positive-scoring matches</option> |
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74 <option value="qframe">qframe = Query frame</option> |
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75 <option value="sframe">sframe = Subject frame</option> |
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76 <option value="qseq">qseq = Aligned part of query sequence</option> |
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77 <option value="sseq">sseq = Aligned part of subject sequence</option> |
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78 <option value="qlen">qlen = Query sequence length</option> |
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79 <option value="slen">slen = Subject sequence length</option> |
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80 <option value="salltitles">salltitles = All subject title(s), separated by a '<>'</option> |
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81 </param> |
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82 <param name="ids_cols" type="select" multiple="true" display="checkboxes" label="Other identifier columns"> |
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83 <option value="qgi">qgi = Query GI</option> |
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84 <option value="qacc">qacc = Query accesion</option> |
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85 <option value="qaccver">qaccver = Query accesion.version</option> |
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86 <option value="sallseqid">sallseqid = All subject Seq-id(s), separated by a ';'</option> |
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87 <option value="sgi">sgi = Subject GI</option> |
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88 <option value="sallgi">sallgi = All subject GIs</option> |
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89 <option value="sacc">sacc = Subject accession</option> |
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90 <option value="saccver">saccver = Subject accession.version</option> |
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91 <option value="sallacc">sallacc = All subject accessions</option> |
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92 <option value="stitle">stitle = Subject Title</option> |
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93 </param> |
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94 <param name="misc_cols" type="select" multiple="true" display="checkboxes" label="Miscellaneous columns"> |
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95 <option value="sstrand">sstrand = Subject Strand</option> |
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96 <!-- Is it really worth including 'frames' given have 'qframe' and 'sframe'? --> |
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97 <option value="frames">frames = Query and subject frames separated by a '/'</option> |
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98 <option value="btop">btop = Blast traceback operations (BTOP)</option> |
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99 <option value="qcovs">qcovs = Query Coverage Per Subject</option> |
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100 <option value="qcovhsp">qcovhsp = Query Coverage Per HSP</option> |
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101 </param> |
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102 <param name="tax_cols" type="select" multiple="true" display="checkboxes" label="Taxonomy columns"> |
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103 <option value="staxids">staxids = unique Subject Taxonomy ID(s), separated by a ';' (in numerical order)</option> |
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104 <!-- TODO, how to handle the taxonomy data file dependency? If missing these give N/A --> |
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105 <option value="sscinames">sscinames = unique Subject Scientific Name(s), separated by a ';'</option> |
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106 <option value="scomnames">scomnames = unique Subject Common Name(s), separated by a ';'</option> |
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107 <option value="sblastnames">sblastnames = unique Subject Blast Name(s), separated by a ';' (in alphabetical order)</option> |
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108 <option value="sskingdoms">sskingdoms = unique Subject Super Kingdom(s), separated by a ';' (in alphabetical order)</option> |
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109 </param> |
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110 </when> |
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111 <when value="5"/> |
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112 <when value="0"/> |
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113 <when value="0 -html"/> |
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114 <when value="2"/> |
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115 <when value="2 -html"/> |
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116 <when value="4"/> |
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117 <when value="4 -html"/> |
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118 </conditional> |
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119 </xml> |
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120 <xml name="input_scoring_matrix"> |
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121 <param name="matrix" type="select" label="Scoring matrix"> |
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122 <option value="BLOSUM90">BLOSUM90</option> |
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123 <option value="BLOSUM80">BLOSUM80</option> |
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124 <option value="BLOSUM62" selected="true">BLOSUM62 (default)</option> |
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125 <option value="BLOSUM50">BLOSUM50</option> |
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126 <option value="BLOSUM45">BLOSUM45</option> |
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127 <option value="PAM250">PAM250</option> |
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128 <option value="PAM70">PAM70</option> |
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129 <option value="PAM30">PAM30</option> |
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130 </param> |
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131 </xml> |
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132 <xml name="input_query_gencode"> |
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133 <param name="query_gencode" type="select" label="Query genetic code"> |
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134 <!-- See http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi for details --> |
12
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135 <option value="1" selected="True">1. Standard</option> |
0
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136 <option value="2">2. Vertebrate Mitochondrial</option> |
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137 <option value="3">3. Yeast Mitochondrial</option> |
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138 <option value="4">4. Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma/Spiroplasma Code</option> |
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139 <option value="5">5. Invertebrate Mitochondrial</option> |
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140 <option value="6">6. Ciliate, Dasycladacean and Hexamita Nuclear Code</option> |
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141 <option value="9">9. Echinoderm Mitochondrial</option> |
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142 <option value="10">10. Euplotid Nuclear</option> |
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143 <option value="11">11. Bacteria and Archaea</option> |
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144 <option value="12">12. Alternative Yeast Nuclear</option> |
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145 <option value="13">13. Ascidian Mitochondrial</option> |
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146 <option value="14">14. Flatworm Mitochondrial</option> |
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147 <option value="15">15. Blepharisma Macronuclear</option> |
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148 <option value="16">16. Chlorophycean Mitochondrial Code</option> |
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149 <option value="21">21. Trematode Mitochondrial Code</option> |
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150 <option value="22">22. Scenedesmus obliquus mitochondrial Code</option> |
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151 <option value="23">23. Thraustochytrium Mitochondrial Code</option> |
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152 <option value="24">24. Pterobranchia mitochondrial code</option> |
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153 </param> |
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154 </xml> |
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155 <xml name="input_db_gencode"> |
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156 <param name="db_gencode" type="select" label="Database/subject genetic code"> |
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157 <!-- See http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi for details --> |
12
d8f2c1f560ec
Uploaded v0.1.08, can search multiple local databases, fixes a pipe problem in blastdbcmd, and minor internal changes.
peterjc
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158 <option value="1" selected="True">1. Standard</option> |
0
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159 <option value="2">2. Vertebrate Mitochondrial</option> |
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160 <option value="3">3. Yeast Mitochondrial</option> |
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161 <option value="4">4. Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma/Spiroplasma Code</option> |
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162 <option value="5">5. Invertebrate Mitochondrial</option> |
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163 <option value="6">6. Ciliate, Dasycladacean and Hexamita Nuclear Code</option> |
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peterjc
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164 <option value="9">9. Echinoderm Mitochondrial</option> |
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165 <option value="10">10. Euplotid Nuclear</option> |
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166 <option value="11">11. Bacteria and Archaea</option> |
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167 <option value="12">12. Alternative Yeast Nuclear</option> |
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168 <option value="13">13. Ascidian Mitochondrial</option> |
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169 <option value="14">14. Flatworm Mitochondrial</option> |
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170 <option value="15">15. Blepharisma Macronuclear</option> |
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peterjc
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171 <option value="16">16. Chlorophycean Mitochondrial Code</option> |
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172 <option value="21">21. Trematode Mitochondrial Code</option> |
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173 <option value="22">22. Scenedesmus obliquus mitochondrial Code</option> |
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174 <option value="23">23. Thraustochytrium Mitochondrial Code</option> |
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175 <option value="24">24. Pterobranchia mitochondrial code</option> |
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176 </param> |
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177 </xml> |
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178 <xml name="input_conditional_nucleotide_db"> |
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179 <conditional name="db_opts"> |
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180 <param name="db_opts_selector" type="select" label="Subject database/sequences"> |
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181 <option value="db" selected="True">Locally installed BLAST database</option> |
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182 <option value="histdb">BLAST database from your history</option> |
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183 <option value="file">FASTA file from your history (see warning note below)</option> |
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184 </param> |
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185 <when value="db"> |
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186 <param name="database" type="select" multiple="true" label="Nucleotide BLAST database"> |
0
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187 <options from_data_table="blastdb" /> |
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188 </param> |
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189 <param name="histdb" type="hidden" value="" /> |
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190 <param name="subject" type="hidden" value="" /> |
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191 </when> |
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192 <when value="histdb"> |
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193 <param name="database" type="hidden" value="" /> |
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194 <param name="histdb" type="data" format="blastdbn" label="Nucleotide BLAST database" /> |
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195 <param name="subject" type="hidden" value="" /> |
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196 </when> |
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197 <when value="file"> |
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198 <param name="database" type="hidden" value="" /> |
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199 <param name="histdb" type="hidden" value="" /> |
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200 <param name="subject" type="data" format="fasta" label="Nucleotide FASTA file to use as database"/> |
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201 </when> |
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202 </conditional> |
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203 </xml> |
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204 <xml name="input_conditional_protein_db"> |
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205 <conditional name="db_opts"> |
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206 <param name="db_opts_selector" type="select" label="Subject database/sequences"> |
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207 <option value="db" selected="True">Locally installed BLAST database</option> |
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208 <option value="histdb">BLAST database from your history</option> |
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|
209 <option value="file">FASTA file from your history (see warning note below)</option> |
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210 </param> |
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211 <when value="db"> |
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peterjc
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212 <param name="database" type="select" multiple="true" label="Protein BLAST database"> |
0
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213 <options from_data_table="blastdb_p" /> |
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214 </param> |
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215 <param name="histdb" type="hidden" value="" /> |
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216 <param name="subject" type="hidden" value="" /> |
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217 </when> |
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218 <when value="histdb"> |
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219 <param name="database" type="hidden" value="" /> |
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220 <param name="histdb" type="data" format="blastdbp" label="Protein BLAST database" /> |
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221 <param name="subject" type="hidden" value="" /> |
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222 </when> |
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223 <when value="file"> |
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224 <param name="database" type="hidden" value="" /> |
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225 <param name="histdb" type="hidden" value="" /> |
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226 <param name="subject" type="data" format="fasta" label="Protein FASTA file to use as database"/> |
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227 </when> |
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228 </conditional> |
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229 </xml> |
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230 <xml name="input_conditional_pssm"> |
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231 <conditional name="db_opts"> |
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232 <param name="db_opts_selector" type="select" label="Protein domain database (PSSM)"> |
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233 <option value="db" selected="True">Locally installed BLAST protein domain database</option> |
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234 <option value="histdb">BLAST protein domain database from your history</option> |
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235 </param> |
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236 <when value="db"> |
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237 <param name="database" type="select" label="Protein domain database"> |
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238 <options from_data_table="blastdb_d" /> |
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239 </param> |
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240 <param name="histdb" type="hidden" value="" /> |
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241 <param name="subject" type="hidden" value="" /> |
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242 </when> |
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243 <when value="histdb"> |
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244 <param name="database" type="hidden" value="" /> |
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245 <param name="histdb" type="data" format="blastdbd" label="Protein domain database" /> |
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246 <param name="subject" type="hidden" value="" /> |
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247 </when> |
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248 </conditional> |
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249 </xml> |
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250 <xml name="input_conditional_choose_db_type"> |
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251 <conditional name="db_opts"> |
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252 <param name="db_type" type="select" label="Type of BLAST database"> |
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253 <option value="nucl" selected="True">Nucleotide</option> |
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254 <option value="prot">Protein</option> |
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255 </param> |
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256 <when value="nucl"> |
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257 <param name="database" type="select" multiple="true" label="Nucleotide BLAST database"> |
0
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258 <options from_data_table="blastdb" /> |
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259 </param> |
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260 </when> |
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261 <when value="prot"> |
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262 <param name="database" type="select" multiple="true" label="Protein BLAST database"> |
0
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263 <options from_data_table="blastdb_p" /> |
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264 </param> |
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265 </when> |
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266 </conditional> |
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267 </xml> |
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268 <xml name="input_parse_deflines"> |
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269 <param name="parse_deflines" type="boolean" label="Should the query and subject defline(s) be parsed?" truevalue="-parse_deflines" falsevalue="" checked="false" help="This affects the formatting of the query/subject ID strings"/> |
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270 </xml> |
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271 <xml name="input_filter_query_default_false"> |
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272 <param name="filter_query" type="boolean" label="Filter out low complexity regions (with SEG)" truevalue="-seg yes" falsevalue="-seg no" checked="false" /> |
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273 </xml> |
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274 <xml name="input_filter_query_default_true"> |
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275 <param name="filter_query" type="boolean" label="Filter out low complexity regions (with SEG)" truevalue="-seg yes" falsevalue="-seg no" checked="true" /> |
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276 </xml> |
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277 <xml name="input_max_hits"> |
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278 <param name="max_hits" type="integer" value="0" label="Maximum hits to show" help="Use zero for default limits"> |
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279 <validator type="in_range" min="0" /> |
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280 </param> |
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281 </xml> |
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282 <xml name="input_evalue"> |
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283 <param name="evalue_cutoff" type="float" size="15" value="0.001" label="Set expectation value cutoff" /> |
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284 </xml> |
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285 <xml name="input_word_size"> |
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286 <param name="word_size" type="integer" value="0" label="Word size for wordfinder algorithm" help="Use zero for default, otherwise minimum 2."> |
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287 <validator type="in_range" min="0" /> |
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288 </param> |
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289 </xml> |
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290 <xml name="input_strand"> |
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291 <param name="strand" type="select" label="Query strand(s) to search against database/subject"> |
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292 <option value="-strand both">Both</option> |
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293 <option value="-strand plus">Plus (forward)</option> |
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294 <option value="-strand minus">Minus (reverse complement)</option> |
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295 </param> |
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296 </xml> |
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297 <xml name="input_qcov_hsp_perc"> |
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298 <param name="qcov_hsp_perc" type="float" value="0" min="0" max="100" label="Minimum query coverage per hsp (percentage, 0 to 100)" help="See also the output column qcovhsp"/> |
0
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299 </xml> |
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300 <xml name="advanced_options"> |
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301 <conditional name="adv_opts"> |
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302 <param name="adv_opts_selector" type="select" label="Advanced Options"> |
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303 <option value="basic" selected="True">Hide Advanced Options</option> |
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304 <option value="advanced">Show Advanced Options</option> |
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305 </param> |
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306 <when value="basic" /> |
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307 <when value="advanced"> |
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308 <yield /> |
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309 </when> |
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310 </conditional> |
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311 </xml> |
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312 <xml name="advanced_optional_id_files"> |
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313 <conditional name="adv_optional_id_files_opts"> |
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314 <param name="adv_optional_id_files_opts_selector" type="select" |
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315 label="Restrict search of database to a given set of ID's" |
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316 help="This feature provides a means to exclude ID's from a BLAST database search. The expectation values in the BLAST results are based upon the sequences actually searched, and not on the underlying database. Note this cannot be used when comparing against a FASTA file."> |
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317 <option value="none" selected="True">No restriction, search the entire database</option> |
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318 <option value="gilist">GI identifiers</option> |
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319 <option value="negative_gilist">Negative GI identifiers</option> |
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320 <option value="seqidlist">Sequence identifiers (SeqId's)</option> |
0
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321 </param> |
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322 <when value="none" /> |
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323 <when value="gilist"> |
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324 <param name="gilist" type="data" format="txt" label="Restrict search of database to GI's listed in this file" |
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325 help="This option is only available for database searches."/> |
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326 </when> |
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327 <when value="negative_gilist"> |
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328 <param name="negative_gilist" type="data" format="txt" label="Restrict search of database to everything except the GI's listed in this file" |
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329 help="This option is only available for database searches."/> |
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330 </when> |
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331 <when value="seqidlist"> |
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332 <param name="seqidlist" type="data" format="txt" label=" Restrict search of database to list of SeqId's" |
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333 help="This option is only available for database searches."/> |
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334 </when> |
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335 </conditional> |
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336 </xml> |
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337 <token name="@ADV_QCOV_HSP_PERC@"> |
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338 #if float(str($adv_opts.qcov_hsp_perc)) > 0: |
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339 -qcov_hsp_perc $adv_opts.qcov_hsp_perc |
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340 #end if |
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341 </token> |
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342 <token name="@ADV_ID_LIST_FILTER@"> |
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343 #if $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'negative_gilist': |
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344 -negative_gilist $adv_opts.adv_optional_id_files_opts.negative_gilist |
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345 #elif $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'gilist': |
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346 -gilist $adv_opts.adv_optional_id_files_opts.gilist |
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347 #elif $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'seqidlist': |
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348 -seqidlist $adv_opts.adv_optional_id_files_opts.seqidlist |
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349 #end if |
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350 </token> |
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351 <token name="@THREADS@">-num_threads "\${GALAXY_SLOTS:-8}"</token> |
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352 <token name="@BLAST_DB_SUBJECT@"> |
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353 #if $db_opts.db_opts_selector == "db": |
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354 -db "${' '.join(str( $db_opts.database.fields.path ).split( ',' ))}" |
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355 #elif $db_opts.db_opts_selector == "histdb": |
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356 -db "${os.path.join($db_opts.histdb.extra_files_path,'blastdb')}" |
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357 #else: |
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358 -subject "$db_opts.subject" |
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359 #end if |
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360 </token> |
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361 <token name="@BLAST_OUTPUT@">-out "$output1" |
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362 ##Set the extended list here so when we add things, saved workflows are not affected |
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363 #if str($output.out_format)=="ext": |
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364 -outfmt "6 std sallseqid score nident positive gaps ppos qframe sframe qseq sseq qlen slen salltitles" |
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365 #elif str($output.out_format)=="cols" |
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366 ##Pick your own columns. Galaxy gives us it comma separated, BLAST+ wants space separated: |
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367 ##TODO - Can we catch the user picking no columns and raise an error here? |
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368 #set cols = (str($output.std_cols)+","+str($output.ext_cols)+","+str($output.ids_cols)+","+str($output.misc_cols)+","+str($output.tax_cols)).replace("None", "").replace(",,", ",").replace(",", " ").strip() |
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369 -outfmt "6 $cols" |
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370 #else: |
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371 -outfmt $output.out_format |
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372 #end if |
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373 </token> |
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374 <token name="@ADV_FILTER_QUERY@">$adv_opts.filter_query</token> |
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375 <token name="@ADV_MAX_HITS@"> |
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376 ## Need int(str(...)) because $adv_opts.max_hits is an InputValueWrapper object not a string |
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377 ## Note -max_target_seqs used to simply override -num_descriptions and -num_alignments |
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378 ## but this was changed in BLAST+ 2.2.27 onwards to force their use (raised with NCBI) |
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379 #if (str($adv_opts.max_hits) and int(str($adv_opts.max_hits)) > 0): |
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380 #if str($output.out_format) in ["6", "ext", "cols", "5"]: |
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381 ## Most output formats use this, including tabular and XML: |
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382 -max_target_seqs $adv_opts.max_hits |
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383 #else |
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384 ## Text and HTML output formats 0-4 currently need this instead: |
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385 -num_descriptions $adv_opts.max_hits -num_alignments $adv_opts.max_hits |
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386 #end if |
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387 #end if |
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388 </token> |
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389 <token name="@ADV_WORD_SIZE@"> |
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390 #if (str($adv_opts.word_size) and int(str($adv_opts.word_size)) > 0): |
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391 -word_size $adv_opts.word_size |
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392 #end if |
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393 $adv_opts.parse_deflines |
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394 </token> |
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395 <!-- @ON_DB_SUBJECT@ is for use with @BLAST_DB_SUBJECT@ --> |
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396 <token name="@ON_DB_SUBJECT@">#if str($db_opts.db_opts_selector)=='db' |
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397 ${db_opts.database} |
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398 #elif str($db_opts.db_opts_selector)=='histdb' |
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399 ${db_opts.histdb.name} |
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400 #else |
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401 ${db_opts.subject.name} |
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402 #end if</token> |
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403 <token name="@REFERENCES@"> |
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404 Peter J. A. Cock, John M. Chilton, Björn Grüning, James E. Johnson, Nicola Soranzo (2015). |
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405 NCBI BLAST+ integrated into Galaxy. *GigaScience* 4:39 |
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406 http://dx.doi.org/10.1186/s13742-015-0080-7 |
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407 |
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408 Christiam Camacho et al. (2009). |
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409 BLAST+: architecture and applications. |
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410 *BMC Bioinformatics* 15;10:421. |
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411 http://dx.doi.org/10.1186/1471-2105-10-421 |
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412 |
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413 This wrapper is available to install into other Galaxy Instances via the Galaxy |
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414 Tool Shed at http://toolshed.g2.bx.psu.edu/view/devteam/ncbi_blast_plus |
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415 </token> |
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416 <xml name="blast_citations"> |
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417 <citations> |
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418 <citation type="doi">10.1186/1471-2105-10-421</citation> |
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419 <citation type="doi">10.1186/s13742-015-0080-7</citation> |
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420 </citations> |
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421 </xml> |
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422 <token name="@OUTPUT_FORMAT@">**Output format** |
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423 |
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424 Because Galaxy focuses on processing tabular data, the default output of this |
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425 tool is tabular. The standard BLAST+ tabular output contains 12 columns: |
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426 |
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427 ====== ========= ============================================ |
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428 Column NCBI name Description |
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429 ------ --------- -------------------------------------------- |
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430 1 qseqid Query Seq-id (ID of your sequence) |
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431 2 sseqid Subject Seq-id (ID of the database hit) |
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432 3 pident Percentage of identical matches |
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433 4 length Alignment length |
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434 5 mismatch Number of mismatches |
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435 6 gapopen Number of gap openings |
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436 7 qstart Start of alignment in query |
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437 8 qend End of alignment in query |
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438 9 sstart Start of alignment in subject (database hit) |
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439 10 send End of alignment in subject (database hit) |
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440 11 evalue Expectation value (E-value) |
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441 12 bitscore Bit score |
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442 ====== ========= ============================================ |
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443 |
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444 The BLAST+ tools can optionally output additional columns of information, |
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445 but this takes longer to calculate. Many commonly used extra columns are |
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446 included by selecting the extended tabular output. The extra columns are |
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447 included *after* the standard 12 columns. This is so that you can write |
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448 workflow filtering steps that accept either the 12 or 25 column tabular |
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449 BLAST output. Galaxy now uses this extended 25 column output by default. |
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450 |
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451 ====== ============= =========================================== |
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452 Column NCBI name Description |
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453 ------ ------------- ------------------------------------------- |
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454 13 sallseqid All subject Seq-id(s), separated by a ';' |
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455 14 score Raw score |
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456 15 nident Number of identical matches |
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457 16 positive Number of positive-scoring matches |
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458 17 gaps Total number of gaps |
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459 18 ppos Percentage of positive-scoring matches |
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460 19 qframe Query frame |
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461 20 sframe Subject frame |
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462 21 qseq Aligned part of query sequence |
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463 22 sseq Aligned part of subject sequence |
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464 23 qlen Query sequence length |
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465 24 slen Subject sequence length |
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466 25 salltitles All subject title(s), separated by a '<>' |
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467 ====== ============= =========================================== |
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468 |
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469 The third option is to customise the tabular output by selecting which |
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470 columns you want, from the standard set of 12, the default set of 25, |
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471 or any of the additional columns BLAST+ offers (including species name). |
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472 |
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473 The fourth option is BLAST XML output, which is designed to be parsed by |
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474 another program, and is understood by some Galaxy tools. |
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475 |
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476 You can also choose several plain text or HTML output formats which are designed to be read by a person (not by another program). |
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477 The HTML versions use basic webpage formatting and can include links to the hits on the NCBI website. |
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478 The pairwise output (the default on the NCBI BLAST website) shows each match as a pairwise alignment with the query. |
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479 The two query anchored outputs show a multiple sequence alignment between the query and all the matches, |
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480 and differ in how insertions are shown (marked as insertions or with gap characters added to the other sequences). |
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481 </token> |
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482 <token name="@FASTA_WARNING@">.. class:: warningmark |
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483 |
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484 You can also search against a FASTA file of subject (target) |
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485 sequences. This is *not* advised because it is slower (only one |
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486 CPU is used), but more importantly gives e-values for pairwise |
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487 searches (very small e-values which will look overly signficiant). |
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488 In most cases you should instead turn the other FASTA file into a |
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489 database first using *makeblastdb* and search against that. |
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490 </token> |
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491 <token name="@SEARCH_TIME_WARNING@">.. class:: warningmark |
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492 |
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493 **Note**. Database searches may take a substantial amount of time. |
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494 For large input datasets it is advisable to allow overnight processing. |
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495 |
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496 ----- |
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497 </token> |
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498 </macros> |