Mercurial > repos > devteam > microsats_mutability

comparison microsats_mutability.py @ 0:0530d2a49487 draft

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Imported from capsule None
author devteam
date Tue, 01 Apr 2014 09:13:04 -0400
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-1:000000000000 0:0530d2a49487
1 #!/usr/bin/env python
2 #Guruprasad Ananda
3 """
4 This tool computes microsatellite mutability for the orthologous microsatellites fetched from 'Extract Orthologous Microsatellites from pair-wise alignments' tool.
5 """
6 import fileinput
7 import string
8 import sys
9 import tempfile
10 from galaxy.tools.util.galaxyops import *
11 from bx.intervals.io import *
12 from bx.intervals.operations import quicksect
13
14 fout = open(sys.argv[2],'w')
15 p_group = int(sys.argv[3]) #primary "group-by" feature
16 p_bin_size = int(sys.argv[4])
17 s_group = int(sys.argv[5]) #sub-group by feature
18 s_bin_size = int(sys.argv[6])
19 mono_threshold = 9
20 non_mono_threshold = 4
21 p_group_cols = [p_group, p_group+7]
22 s_group_cols = [s_group, s_group+7]
23 num_generations = int(sys.argv[7])
24 region = sys.argv[8]
25 int_file = sys.argv[9]
26 if int_file != "None": #User has specified an interval file
27 try:
28 fint = open(int_file, 'r')
29 dbkey_i = sys.argv[10]
30 chr_col_i, start_col_i, end_col_i, strand_col_i = parse_cols_arg( sys.argv[11] )
31 except:
32 stop_err("Unable to open input Interval file")
33
34
35 def stop_err(msg):
36 sys.stderr.write(msg)
37 sys.exit()
38
39
40 def reverse_complement(text):
41 DNA_COMP = string.maketrans( "ACGTacgt", "TGCAtgca" )
42 comp = [ch for ch in text.translate(DNA_COMP)]
43 comp.reverse()
44 return "".join(comp)
45
46
47 def get_unique_elems(elems):
48 seen = set()
49 return[x for x in elems if x not in seen and not seen.add(x)]
50
51
52 def get_binned_lists(uniqlist, binsize):
53 binnedlist = []
54 uniqlist.sort()
55 start = int(uniqlist[0])
56 bin_ind = 0
57 l_ind = 0
58 binnedlist.append([])
59 while l_ind < len(uniqlist):
60 elem = int(uniqlist[l_ind])
61 if elem in range(start, start+binsize):
62 binnedlist[bin_ind].append(elem)
63 else:
64 start += binsize
65 bin_ind += 1
66 binnedlist.append([])
67 binnedlist[bin_ind].append(elem)
68 l_ind += 1
69 return binnedlist
70
71
72 def fetch_weight(H, C, t):
73 if (H-(C-H)) < t:
74 return 2.0
75 else:
76 return 1.0
77
78
79 def mutabilityEstimator(repeats1, repeats2, thresholds):
80 mut_num = 0.0 #Mutability Numerator
81 mut_den = 0.0 #Mutability denominator
82 for ind, H in enumerate(repeats1):
83 C = repeats2[ind]
84 t = thresholds[ind]
85 w = fetch_weight(H, C, t)
86 mut_num += ((H-C)*(H-C)*w)
87 mut_den += w
88 return [mut_num, mut_den]
89
90
91 def output_writer(blk, blk_lines):
92 global winspecies, speciesind
93 all_elems_1 = []
94 all_elems_2 = []
95 all_s_elems_1 = []
96 all_s_elems_2 = []
97 for bline in blk_lines:
98 if not(bline):
99 continue
100 items = bline.split('\t')
101 seq1 = items[1]
102 seq2 = items[8]
103 if p_group_cols[0] == 6:
104 items[p_group_cols[0]] = int(items[p_group_cols[0]])
105 items[p_group_cols[1]] = int(items[p_group_cols[1]])
106 if s_group_cols[0] == 6:
107 items[s_group_cols[0]] = int(items[s_group_cols[0]])
108 items[s_group_cols[1]] = int(items[s_group_cols[1]])
109 all_elems_1.append(items[p_group_cols[0]]) #primary col elements for species 1
110 all_elems_2.append(items[p_group_cols[1]]) #primary col elements for species 2
111 if s_group_cols[0] != -1: #sub-group is not None
112 all_s_elems_1.append(items[s_group_cols[0]]) #secondary col elements for species 1
113 all_s_elems_2.append(items[s_group_cols[1]]) #secondary col elements for species 2
114 uniq_elems_1 = get_unique_elems(all_elems_1)
115 uniq_elems_2 = get_unique_elems(all_elems_2)
116 if s_group_cols[0] != -1:
117 uniq_s_elems_1 = get_unique_elems(all_s_elems_1)
118 uniq_s_elems_2 = get_unique_elems(all_s_elems_2)
119 mut1 = {}
120 mut2 = {}
121 count1 = {}
122 count2 = {}
123 """
124 if p_group_cols[0] == 7: #i.e. the option chosen is group-by unit(AG, GTC, etc)
125 uniq_elems_1 = get_unique_units(j.sort(lambda x, y: len(x)-len(y)))
126 """
127 if p_group_cols[0] == 6: #i.e. the option chosen is group-by repeat number.
128 uniq_elems_1 = get_binned_lists( uniq_elems_1, p_bin_size )
129 uniq_elems_2 = get_binned_lists( uniq_elems_2, p_bin_size )
130
131 if s_group_cols[0] == 6: #i.e. the option chosen is subgroup-by repeat number.
132 uniq_s_elems_1 = get_binned_lists( uniq_s_elems_1, s_bin_size )
133 uniq_s_elems_2 = get_binned_lists( uniq_s_elems_2, s_bin_size )
134
135 for pitem1 in uniq_elems_1:
136 #repeats1 = []
137 #repeats2 = []
138 thresholds = []
139 if s_group_cols[0] != -1: #Sub-group by feature is not None
140 for sitem1 in uniq_s_elems_1:
141 repeats1 = []
142 repeats2 = []
143 if type(sitem1) == type(''):
144 sitem1 = sitem1.strip()
145 for bline in blk_lines:
146 belems = bline.split('\t')
147 if type(pitem1) == list:
148 if p_group_cols[0] == 6:
149 belems[p_group_cols[0]] = int(belems[p_group_cols[0]])
150 if belems[p_group_cols[0]] in pitem1:
151 if belems[s_group_cols[0]] == sitem1:
152 repeats1.append(int(belems[6]))
153 repeats2.append(int(belems[13]))
154 if belems[4] == 'mononucleotide':
155 thresholds.append(mono_threshold)
156 else:
157 thresholds.append(non_mono_threshold)
158 mut1[str(pitem1)+'\t'+str(sitem1)] = mutabilityEstimator( repeats1, repeats2, thresholds )
159 if region == 'align':
160 count1[str(pitem1)+'\t'+str(sitem1)] = min( sum(repeats1), sum(repeats2) )
161 else:
162 if winspecies == 1:
163 count1["%s\t%s" % ( pitem1, sitem1 )] = sum(repeats1)
164 elif winspecies == 2:
165 count1["%s\t%s" % ( pitem1, sitem1 )] = sum(repeats2)
166 else:
167 if type(sitem1) == list:
168 if s_group_cols[0] == 6:
169 belems[s_group_cols[0]] = int(belems[s_group_cols[0]])
170 if belems[p_group_cols[0]] == pitem1 and belems[s_group_cols[0]] in sitem1:
171 repeats1.append(int(belems[6]))
172 repeats2.append(int(belems[13]))
173 if belems[4] == 'mononucleotide':
174 thresholds.append(mono_threshold)
175 else:
176 thresholds.append(non_mono_threshold)
177 mut1["%s\t%s" % ( pitem1, sitem1 )] = mutabilityEstimator( repeats1, repeats2, thresholds )
178 if region == 'align':
179 count1[str(pitem1)+'\t'+str(sitem1)] = min( sum(repeats1), sum(repeats2) )
180 else:
181 if winspecies == 1:
182 count1[str(pitem1)+'\t'+str(sitem1)] = sum(repeats1)
183 elif winspecies == 2:
184 count1[str(pitem1)+'\t'+str(sitem1)] = sum(repeats2)
185 else:
186 if belems[p_group_cols[0]] == pitem1 and belems[s_group_cols[0]] == sitem1:
187 repeats1.append(int(belems[6]))
188 repeats2.append(int(belems[13]))
189 if belems[4] == 'mononucleotide':
190 thresholds.append(mono_threshold)
191 else:
192 thresholds.append(non_mono_threshold)
193 mut1["%s\t%s" % ( pitem1, sitem1 )] = mutabilityEstimator( repeats1, repeats2, thresholds )
194 if region == 'align':
195 count1[str(pitem1)+'\t'+str(sitem1)] = min( sum(repeats1), sum(repeats2) )
196 else:
197 if winspecies == 1:
198 count1["%s\t%s" % ( pitem1, sitem1 )] = sum(repeats1)
199 elif winspecies == 2:
200 count1["%s\t%s" % ( pitem1, sitem1 )] = sum(repeats2)
201 else: #Sub-group by feature is None
202 for bline in blk_lines:
203 belems = bline.split('\t')
204 if type(pitem1) == list:
205 #print >> sys.stderr, "item: " + str(item1)
206 if p_group_cols[0] == 6:
207 belems[p_group_cols[0]] = int(belems[p_group_cols[0]])
208 if belems[p_group_cols[0]] in pitem1:
209 repeats1.append(int(belems[6]))
210 repeats2.append(int(belems[13]))
211 if belems[4] == 'mononucleotide':
212 thresholds.append(mono_threshold)
213 else:
214 thresholds.append(non_mono_threshold)
215 else:
216 if belems[p_group_cols[0]] == pitem1:
217 repeats1.append(int(belems[6]))
218 repeats2.append(int(belems[13]))
219 if belems[4] == 'mononucleotide':
220 thresholds.append(mono_threshold)
221 else:
222 thresholds.append(non_mono_threshold)
223 mut1["%s" % (pitem1)] = mutabilityEstimator( repeats1, repeats2, thresholds )
224 if region == 'align':
225 count1["%s" % (pitem1)] = min( sum(repeats1), sum(repeats2) )
226 else:
227 if winspecies == 1:
228 count1[str(pitem1)] = sum(repeats1)
229 elif winspecies == 2:
230 count1[str(pitem1)] = sum(repeats2)
231
232 for pitem2 in uniq_elems_2:
233 #repeats1 = []
234 #repeats2 = []
235 thresholds = []
236 if s_group_cols[0] != -1: #Sub-group by feature is not None
237 for sitem2 in uniq_s_elems_2:
238 repeats1 = []
239 repeats2 = []
240 if type(sitem2)==type(''):
241 sitem2 = sitem2.strip()
242 for bline in blk_lines:
243 belems = bline.split('\t')
244 if type(pitem2) == list:
245 if p_group_cols[0] == 6:
246 belems[p_group_cols[1]] = int(belems[p_group_cols[1]])
247 if belems[p_group_cols[1]] in pitem2 and belems[s_group_cols[1]] == sitem2:
248 repeats2.append(int(belems[13]))
249 repeats1.append(int(belems[6]))
250 if belems[4] == 'mononucleotide':
251 thresholds.append(mono_threshold)
252 else:
253 thresholds.append(non_mono_threshold)
254 mut2["%s\t%s" % ( pitem2, sitem2 )] = mutabilityEstimator( repeats2, repeats1, thresholds )
255 #count2[str(pitem2)+'\t'+str(sitem2)]=len(repeats2)
256 if region == 'align':
257 count2["%s\t%s" % ( pitem2, sitem2 )] = min( sum(repeats1), sum(repeats2) )
258 else:
259 if winspecies == 1:
260 count2["%s\t%s" % ( pitem2, sitem2 )] = len(repeats2)
261 elif winspecies == 2:
262 count2["%s\t%s" % ( pitem2, sitem2 )] = len(repeats1)
263 else:
264 if type(sitem2) == list:
265 if s_group_cols[0] == 6:
266 belems[s_group_cols[1]] = int(belems[s_group_cols[1]])
267 if belems[p_group_cols[1]] == pitem2 and belems[s_group_cols[1]] in sitem2:
268 repeats2.append(int(belems[13]))
269 repeats1.append(int(belems[6]))
270 if belems[4] == 'mononucleotide':
271 thresholds.append(mono_threshold)
272 else:
273 thresholds.append(non_mono_threshold)
274 mut2["%s\t%s" % ( pitem2, sitem2 )] = mutabilityEstimator( repeats2, repeats1, thresholds )
275 if region == 'align':
276 count2["%s\t%s" % ( pitem2, sitem2 )] = min( sum(repeats1), sum(repeats2) )
277 else:
278 if winspecies == 1:
279 count2["%s\t%s" % ( pitem2, sitem2 )] = len(repeats2)
280 elif winspecies == 2:
281 count2["%s\t%s" % ( pitem2, sitem2 )] = len(repeats1)
282 else:
283 if belems[p_group_cols[1]] == pitem2 and belems[s_group_cols[1]] == sitem2:
284 repeats1.append(int(belems[13]))
285 repeats2.append(int(belems[6]))
286 if belems[4] == 'mononucleotide':
287 thresholds.append(mono_threshold)
288 else:
289 thresholds.append(non_mono_threshold)
290 mut2["%s\t%s" % ( pitem2, sitem2 )] = mutabilityEstimator( repeats2, repeats1, thresholds )
291 if region == 'align':
292 count2["%s\t%s" % ( pitem2, sitem2 )] = min( sum(repeats1), sum(repeats2) )
293 else:
294 if winspecies == 1:
295 count2["%s\t%s" % ( pitem2, sitem2 )] = len(repeats2)
296 elif winspecies == 2:
297 count2["%s\t%s" % ( pitem2, sitem2 )] = len(repeats1)
298 else: #Sub-group by feature is None
299 for bline in blk_lines:
300 belems = bline.split('\t')
301 if type(pitem2) == list:
302 if p_group_cols[0] == 6:
303 belems[p_group_cols[1]] = int(belems[p_group_cols[1]])
304 if belems[p_group_cols[1]] in pitem2:
305 repeats2.append(int(belems[13]))
306 repeats1.append(int(belems[6]))
307 if belems[4] == 'mononucleotide':
308 thresholds.append(mono_threshold)
309 else:
310 thresholds.append(non_mono_threshold)
311 else:
312 if belems[p_group_cols[1]] == pitem2:
313 repeats2.append(int(belems[13]))
314 repeats1.append(int(belems[6]))
315 if belems[4] == 'mononucleotide':
316 thresholds.append(mono_threshold)
317 else:
318 thresholds.append(non_mono_threshold)
319 mut2["%s" % (pitem2)] = mutabilityEstimator( repeats2, repeats1, thresholds )
320 if region == 'align':
321 count2["%s" % (pitem2)] = min( sum(repeats1), sum(repeats2) )
322 else:
323 if winspecies == 1:
324 count2["%s" % (pitem2)] = sum(repeats2)
325 elif winspecies == 2:
326 count2["%s" % (pitem2)] = sum(repeats1)
327 for key in mut1.keys():
328 if key in mut2.keys():
329 mut = (mut1[key][0]+mut2[key][0])/(mut1[key][1]+mut2[key][1])
330 count = count1[key]
331 del mut2[key]
332 else:
333 unit_found = False
334 if p_group_cols[0] == 7 or s_group_cols[0] == 7: #if it is Repeat Unit (AG, GCT etc.) check for reverse-complements too
335 if p_group_cols[0] == 7:
336 this, other = 0, 1
337 else:
338 this, other = 1, 0
339 groups1 = key.split('\t')
340 mutn = mut1[key][0]
341 mutd = mut1[key][1]
342 count = 0
343 for key2 in mut2.keys():
344 groups2 = key2.split('\t')
345 if groups1[other] == groups2[other]:
346 if groups1[this] in groups2[this]*2 or reverse_complement(groups1[this]) in groups2[this]*2:
347 #mut = (mut1[key][0]+mut2[key2][0])/(mut1[key][1]+mut2[key2][1])
348 mutn += mut2[key2][0]
349 mutd += mut2[key2][1]
350 count += int(count2[key2])
351 unit_found = True
352 del mut2[key2]
353 #break
354 if unit_found:
355 mut = mutn/mutd
356 else:
357 mut = mut1[key][0]/mut1[key][1]
358 count = count1[key]
359 mut = "%.2e" % (mut/num_generations)
360 if region == 'align':
361 print >> fout, str(blk) + '\t'+seq1 + '\t' + seq2 + '\t' +key.strip()+ '\t'+str(mut) + '\t'+ str(count)
362 elif region == 'win':
363 fout.write("%s\t%s\t%s\t%s\n" % ( blk, key.strip(), mut, count ))
364 fout.flush()
365
366 #catch any remaining repeats, for instance if the orthologous position contained different repeat units
367 for remaining_key in mut2.keys():
368 mut = mut2[remaining_key][0]/mut2[remaining_key][1]
369 mut = "%.2e" % (mut/num_generations)
370 count = count2[remaining_key]
371 if region == 'align':
372 print >> fout, str(blk) + '\t'+seq1 + '\t'+seq2 + '\t'+remaining_key.strip()+ '\t'+str(mut)+ '\t'+ str(count)
373 elif region == 'win':
374 fout.write("%s\t%s\t%s\t%s\n" % ( blk, remaining_key.strip(), mut, count ))
375 fout.flush()
376 #print >> fout, blk + '\t'+remaining_key.strip()+ '\t'+str(mut)+ '\t'+ str(count)
377
378
379 def counter(node, start, end, report_func):
380 if start <= node.start < end and start < node.end <= end:
381 report_func(node)
382 if node.right:
383 counter(node.right, start, end, report_func)
384 if node.left:
385 counter(node.left, start, end, report_func)
386 elif node.start < start and node.right:
387 counter(node.right, start, end, report_func)
388 elif node.start >= end and node.left and node.left.maxend > start:
389 counter(node.left, start, end, report_func)
390
391
392 def main():
393 infile = sys.argv[1]
394
395 for i, line in enumerate( file ( infile )):
396 line = line.rstrip('\r\n')
397 if len( line )>0 and not line.startswith( '#' ):
398 elems = line.split( '\t' )
399 break
400 if i == 30:
401 break # Hopefully we'll never get here...
402
403 if len( elems ) != 15:
404 stop_err( "This tool only works on tabular data output by 'Extract Orthologous Microsatellites from pair-wise alignments' tool. The data in your input dataset is either missing or not formatted properly." )
405 global winspecies, speciesind
406 if region == 'win':
407 if dbkey_i in elems[1]:
408 winspecies = 1
409 speciesind = 1
410 elif dbkey_i in elems[8]:
411 winspecies = 2
412 speciesind = 8
413 else:
414 stop_err("The species build corresponding to your interval file is not present in the Microsatellite file.")
415
416 fin = open(infile, 'r')
417 skipped = 0
418 linestr = ""
419
420 if region == 'win':
421 msats = NiceReaderWrapper( fileinput.FileInput( infile ),
422 chrom_col = speciesind,
423 start_col = speciesind+1,
424 end_col = speciesind+2,
425 strand_col = -1,
426 fix_strand = True)
427 msatTree = quicksect.IntervalTree()
428 for item in msats:
429 if type( item ) is GenomicInterval:
430 msatTree.insert( item, msats.linenum, item.fields )
431
432 for iline in fint:
433 try:
434 iline = iline.rstrip('\r\n')
435 if not(iline) or iline == "":
436 continue
437 ielems = iline.strip("\r\n").split('\t')
438 ichr = ielems[chr_col_i]
439 istart = int(ielems[start_col_i])
440 iend = int(ielems[end_col_i])
441 isrc = "%s.%s" % ( dbkey_i, ichr )
442 if isrc not in msatTree.chroms:
443 continue
444 result = []
445 root = msatTree.chroms[isrc] #root node for the chrom
446 counter(root, istart, iend, lambda node: result.append( node ))
447 if not(result):
448 continue
449 tmpfile1 = tempfile.NamedTemporaryFile('wb+')
450 for node in result:
451 tmpfile1.write("%s\n" % "\t".join( node.other ))
452
453 tmpfile1.seek(0)
454 output_writer(iline, tmpfile1.readlines())
455 except:
456 skipped += 1
457 if skipped:
458 print "Skipped %d intervals as invalid." % (skipped)
459 elif region == 'align':
460 if s_group_cols[0] != -1:
461 print >> fout, "#Window\tSpecies_1\tSpecies_2\tGroupby_Feature\tSubGroupby_Feature\tMutability\tCount"
462 else:
463 print >> fout, "#Window\tSpecies_1\tWindow_Start\tWindow_End\tSpecies_2\tGroupby_Feature\tMutability\tCount"
464 prev_bnum = -1
465 try:
466 for line in fin:
467 line = line.strip("\r\n")
468 if not(line) or line == "":
469 continue
470 elems = line.split('\t')
471 try:
472 assert int(elems[0])
473 assert len(elems) == 15
474 except:
475 continue
476 new_bnum = int(elems[0])
477 if new_bnum != prev_bnum:
478 if prev_bnum != -1:
479 output_writer(prev_bnum, linestr.strip().replace('\r','\n').split('\n'))
480 linestr = line + "\n"
481 else:
482 linestr += line
483 linestr += "\n"
484 prev_bnum = new_bnum
485 output_writer(prev_bnum, linestr.strip().replace('\r','\n').split('\n'))
486 except Exception, ea:
487 print >> sys.stderr, ea
488 skipped += 1
489 if skipped:
490 print "Skipped %d lines as invalid." % (skipped)
491
492
493 if __name__ == "__main__":
494 main()