Mercurial > repos > devteam > lastz
changeset 2:8e9252994649 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
author | iuc |
---|---|
date | Fri, 02 Mar 2018 12:06:35 -0500 |
parents | 3c13c9c09ad9 |
children | c3767eaae954 |
files | lastz.xml lastz_d.xml lastz_macros.xml lastz_wrapper.py lastz_wrapper.xml test-data/b1.fasta test-data/chrM_human.fa test-data/chrM_mouse.fa test-data/lastz_census.txt test-data/lastz_d_ctrl_file.txt test-data/lastz_d_test1.out test-data/lastz_d_test2.out test-data/lastz_diffs.txt test-data/lastz_diffs_ref_name.txt test-data/lastz_maf.txt test-data/lastz_seqs.loc test-data/lastz_wrapper_out2.sam test-data/lastz_wrapper_out3.tabular test-data/lastz_wrapper_out4.tabular test-data/score_file.txt test-data/test1.out test-data/test2.out test-data/test3.out tool_data_table_conf.xml.test tool_dependencies.xml |
diffstat | 25 files changed, 1572 insertions(+), 10197 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/lastz.xml Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,685 @@ +<tool id="lastz_wrapper_2" name="LASTZ" version="1.3"> + <description>: align long sequences</description> + <macros> + <import>lastz_macros.xml</import> + </macros> + <requirements> + <requirement type="package" version="@LASTZ_CONDA_VERSION@">lastz</requirement> + <requirement type="package" version="1.7">samtools</requirement> + <requirement type="package" version="3.4.2">r-base</requirement> + </requirements> + <command detect_errors="exit_code"><![CDATA[ + lastz + @TARGET_INPUT_COMMAND_LINE@ + ## If --self is set: perform self alignment and ignore the query + #if $where_to_look.self: + '${where_to_look.self}' + #if $where_to_look.nomirror + '${where_to_look.nomirror}' + #end if + #else: + '${query}' + #end if + +## WHERE TO LOOK -------------------------------- + + '${where_to_look.strand}' + #if str( $where_to_look.qhsplimit.qhsplimit_selector ) == "yes": + #if $where_to_look.qhsplimit.qhsplimit_n: + #if $where_to_look.qhsplimit.qhsplimit_nowarn: + #if $where_to_look.qhsplimit.qhsplimit_keep: + '--queryhsplimit=${where_to_look.qhsplimit.qhsplimit_keep},${where_to_look.qhsplimit.qhsplimit_nowarn}:${where_to_look.qhsplimit.qhsplimit_n}' + #else: + '--queryhsplimit=${where_to_look.qhsplimit.qhsplimit_nowarn}:${where_to_look.qhsplimit.qhsplimit_n}' + #end if + #else: + '--queryhsplimit=${where_to_look.qhsplimit.qhsplimit_n}' + #end if + #end if + #end if + + #if $where_to_look.qhspbest: + '--queryhspbest=${where_to_look.qhspbest}' + #end if + + #if str( $where_to_look.qdepth.qdepth_selector ) == "yes": + #if $where_to_look.qdepth.qdepth_n: + #if $where_to_look.qdepth.qdepth_nowarn and not $where_to_look.qdepth.qdepth_keep: + '--querydepth=${where_to_look.qdepth.qdepth_nowarn}:${where_to_look.qdepth.qdepth_n}' + #elif not $where_to_look.qdepth.qdepth_nowarn and $where_to_look.qdepth.qdepth_keep: + '--querydepth=${where_to_look.qdepth.qdepth_keep}:${where_to_look.qdepth.qdepth_n}' + #elif $where_to_look.qdepth.qdepth_nowarn and $where_to_look.qdepth.qdepth_keep: + '--querydepth=${where_to_look.qdepth.qdepth_keep},${where_to_look.qdepth.qdepth_nowarn}:${where_to_look.qdepth.qdepth_n}' + #else: + '--querydepth=${where_to_look.qdepth.qdepth_n}' + #end if + #end if + #end if + +## SCORING -------------------------------- + + #if $scoring.score_file: + '--scores=${scoring.score_file}' + #end if + + #if str( $scoring.match.match_selector ) == "yes": + #if $scoring.match.match_reward: + #if $scoring.match.match_penalty: + '--match=${scoring.match.match_reward},${scoring.match.match_penalty}' + #else: + '--match=${scoring.match.match_reward}' + #end if + #end if + #end if + #if str( $scoring.gap.gap_selector ) == "yes": + #if $scoring.gap.gap_extend: + #if $scoring.gap.gap_open: + '--gap=${scoring.gap.gap_open},${scoring.gap.gap_extend}' + #else: + '--gap=${scoring.gap.gap_extend}' + #end if + #end if + #end if + #if $scoring.ambigN: + '${scoring.ambigN}' + #end if + #if $scoring.ambigIUPAC: + '${scoring.ambigIUPAC}' + #end if + +## SEEDING -------------------------------- + + #if str( $seeding.seed.seed_selector ) == "pre_set": + '${seeding.seed.pre_set_seeds}' + #elif str( $seeding.seed.seed_selector ) == "len": + '--seed=match${seeding.seed.seed_match}' + #elif str( $seeding.seed.seed_selector ) == "half_len": + '--seed=half${seeding.seed.seed_half}' + #elif str( $seeding.seed.seed_selector ) == "pattern": + '--seed=${seeding.seed.pattern}' + #end if + #if str( $seeding.transitions ) != "--transition": + '${seeding.transitions}' + #end if + #if str( $seeding.seed_filer.seed_filer_selector ) == "yes": + #if $seeding.seed_filer.filter_match: + #if $seeding.seed_filer.filter_tr: + '--filter=${seeding.seed_filer.filter_tr},${seeding.seed_filer.filter_match}' + #else: + '--filter=${seeding.seed_filer.filter_match}' + #end if + #end if + #end if + +## FINDING HSPs -------------------------------- + + #if $hsp.gfextend: + '${hsp.gfextend}' + #end if + #if $hsp.nogfextend + '${hsp.nogfextend}' + #end if + #if str( $hsp.hsp_method.hsp_method_selector ) == "match": + + #if $hsp.hsp_method.exact: + '--exact=${hsp.hsp_method.exact}' + #end if + #elif str( $hsp.hsp_method.hsp_method_selector ) == "mismatch": + + #if $hsp.hsp_method.mismatch_count and $hsp.hsp_method.mismatch_length: + '--mismatch=${hsp.hsp_method.mismatch_count},${hsp.hsp_method.mismatch_length}' + #end if + #elif str( $hsp.hsp_method.hsp_method_selector ) == "x": + + #if $hsp.hsp_method.xdrop: + '--xdrop=${hsp.hsp_method.xdrop}' + #end if + + #if $hsp.hsp_method.hspthresh: + '--hspthresh=${hsp.hsp_method.hspthresh}' + #end if + + #if $hsp.hsp_method.hspthresh_top: + '--hspthresh=top${hsp.hsp_method.hspthresh_top}' + #end if + + #if $hsp.hsp_method.hspthresh_top_percent: + '--hspthresh=top${hsp.hsp_method.hspthresh_top_percent}%' + #end if + #end if + #if $hsp.entropy: + '${hsp.entropy}' + #end if + #if $hsp.entropy_report: + '${hsp.entropy_report}' + #end if + #if $hsp.noentropy: + '${hsp.noentropy}' + #end if + +## CHAINING -------------------------------- + + #if $chaining.chain: + '${chaining.chain}' + #end if + #if str( $chaining.chaning_penalties.chaning_penalties_selector ) == "yes": + #if $chaining.chaning_penalties.diag and $chaining.chaning_penalties.anti: + '--chain=${chaining.chaning_penalties.diag},${chaining.chaning_penalties.anti}' + #end if + #end if + +## GAPPED EXTENSION -------------------------------- + + #if $gap_ext.gapped: + '${gap_ext.gapped}' + #end if + #if $gap_ext.nogapped: + '${gap_ext.nogapped}' + #end if + #if $gap_ext.ydrop: + '--ydrop=${gap_ext.ydrop}' + #end if + #if $gap_ext.noytrim: + '${gap_ext.noytrim}' + #end if + #if $gap_ext.gappedthresh: + '--gappedthresh=${gap_ext.gappedthresh}' + #end if + #if $gap_ext.allgappedbounds: + '${gap_ext.allgappedbounds}' + #end if + +## FILTERING -------------------------------- + + #if $filters.identity.id_min: + #if $filters.identity.id_max: + '--filter=identity:${filters.identity.id_min}..${filters.identity.id_max}' + #else: + '--filter=identity:${filters.identity.id_min}' + #end if + #end if + #if $filters.continuity.cont_min: + #if $filters.continuity.cont_max: + '--filter=continuity:${filters.continuity.cont_min}..${filters.continuity.cont_max}' + #else: + '--filter=continuity:${filters.continuity.cont_min}' + #end if + #end if + #if $filters.coverage.cov_min: + #if $filters.coverage.cov_max: + '--filter=coverage:${filters.coverage.cov_min}..${filters.coverage.cov_max}' + #else: + '--filter=coverage:${filters.coverage.cov_min}' + #end if + #end if + #if $filters.filter_nmatch: + '--filter=nmatch:${filters.filter_nmatch}' + #end if + #if $filters.filter_nmatch_percent: + '--filter=nmatch:${filters.filter_nmatch_percent}%' + #end if + #if $filters.filter_nmismatch: + '--filter=nmismatch:0..${filters.filter_nmismatch}' + #end if + #if $filters.filter_ngap: + '--filter=ngap:0..${filters.filter_ngap}' + #end if + #if $filters.filter_cgap: + '--filter=cgap:0..${filters.filter_cgap}' + #end if + #if $filters.notrivial: + '${filters.notrivial}' + #end if + +## INTERPOLATION -------------------------------- + + #if $interpolation.inner: + '--inner=${interpolation.inner}' + #end if + +## OUTPUT FORMATS -------------------------------- + + #if str( $output_format.out.format ) == "bam": + '--format=${output_format.out.bam_options}' + #elif str( $output_format.out.format ) == "general_def": + --format=general- + #elif str( $output_format.out.format ) == "maf": + '--format=${output_format.out.maf_type}' + #elif str( $output_format.out.format ) == "blastn": + --format=BLASTN- + #elif str( $output_format.out.format ) == "general_full": + '--format=general-:${output_format.out.fields}' + #end if + --action:target=multiple + --rdotplot=plot.r + #if str( $output_format.out.format ) == "bam": + | samtools sort -@\${GALAXY_SLOTS:-2} -O bam -o '${output}' && + #else: + > '${output}' && + #end if + Rscript $r_plot > /dev/null 2>&1 + + ]]> + </command> + <configfiles> + <configfile name="r_plot"> + <![CDATA[ + #!/usr/bin/env Rscript + ## Setup R error handling to go to stderr + options( show.error.messages=F, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) + ## Only produce image when 'plot.r' actually contains data + if(file.info('plot.r')\$size>0){ + x <- read.table("plot.r", as.is=TRUE) + png("${out_plot}", width=640, height=640, res=72) + plot(x, type='l', xlab="target", ylab="query", main="plot") + dev.off() + } + ]]> + </configfile> + </configfiles> + <inputs> + <expand macro="target_input"/> + <param name="query" format="fasta,fastq" type="data" label="Select QUERY sequence(s)" help="These are the sequences that you are aligning against TARGET"/> + + <section name="where_to_look" expanded="False" title="Where to look"> + <param name="strand" type="select" display="radio" label="which strand to search" argument="--strand" help="Search both strands or choose plus or minus"> + <option value="--strand=both" selected="True">Both</option> + <option value="--strand=plus">Plus</option> + <option value="--strand=minus">Minus</option> + </param> + <param name="self" type="boolean" display="radio" truevalue="--self" falsevalue="" checked="False" argument="--self" label="Perform a self-alignment: the target sequence is also the query." help="Computation is more efficient than it would be without this option, since only one of each mirror-image pair of alignment blocks is processed (the other, redundant one is skipped during processing, but re-created in the output). Also, the trivial self-alignment block along the main diagonal is omitted from the output. THIS OPTION CANNOT BE USED IF THE TARGET IS COMPRIZED OF MULTIPLE SEQUENCES"/> + <param name="nomirror" type="boolean" display="radio" truevalue="--nomirror" falsevalue="" checked="False" label="Inhibit the re-creation of mirror-image alignments." argument="--nomirror" help="Output consists of only one copy of each meaningful alignment block in a self-alignment. This option is only applicable when the ‑‑self option is used."/> + <conditional name="qhsplimit"> + <param name="qhsplimit_selector" type="select" display="radio" label="Set HSP limit" argument="--queryhsplimit"> + <option value="yes">Yes</option> + <option value="no" selected="true">No</option> + </param> + <when value="yes"> + <param name="qhsplimit_n" optional="true" type="integer" label="Discard queries that have more than N HSPs" help="Any queries that exceed this limit are reported as a warning (to stderr), and no alignments are reported. This is useful for mapping reads to a reference genome, when some reads align to too many places in the reference."/> + <param name="qhsplimit_nowarn" type="boolean" truevalue="nowarn" display="radio" checked="False" label=" Withhold warnings for queries that exceed the limit set above"/> + <param name="qhsplimit_keep" type="boolean" truevalue="keep" display="radio" checked="False" label="Keep queries that exceed the limit and supress warnings" help="For such a query, the first N HSPs found are passed along to downstream processing. Note that the HSPs reported are not the best N HSPs. They are simply the first N found; they very likely have a positional bias."/> + </when> + <when value="no"> + <!-- Do nothing --> + </when> + </conditional> + <param name="qhspbest" type="integer" optional="true" label="For queries that have more than N HSPs, discard any HSPs that score below the Nth best." help="This is useful for mapping reads to a reference genome, when some reads align to too many places in the reference."/> + <conditional name="qdepth"> + <param name="qdepth_selector" type="select" display="radio" label="Set ratio of aligned bases to query length" argument="--querydepth"> + <option value="yes">Yes</option> + <option value="no" selected="true">No</option> + </param> + <when value="yes"> + <param name="qdepth_n" optional="true" type="integer" label="Stop processing gapped alignments for a query/strand if its ratio of aligned bases to query length exceeds" help="This is a real number that corresponds to a depth of coverage threshold. For example, a value of 5.0 would cause termination once a query/strand has an average of five alignments for every base in the query. The numerator is the number of matches or substitutions (but not gaps); the denominator is the length of the query sequence. The purpose of this option is one of saving time. It is useful for automatically terminating the processing of queries with high repeat content, for which other methods of dealing with repetitive content fail. Moreover, back-end filtering options are not considered. In other words, matches are counted for any alignment that meets the scoring threshold, regardless of whether that alignment would be reported. The justification is that we are trying to abort the processing of queries that have too many bounding alignments in the DP matrix, and back-end filtering occurs later in the process."/> + <param name="qdepth_keep" type="boolean" truevalue="keep" display="radio" checked="False" label="Warnings for queries that exceed the limit are witheld" help="Note that the alignments reported are not guaranteed to be the highest scoring alignments that would achieve the threshold. They are simply the first alignments found. In other words, the purpose of this option is one of saving time, not one of finding optimal alignments."/> + <param name="qdepth_nowarn" type="boolean" truevalue="nowarn" display="radio" checked="False" label="Same as above but any alignments discovered for this query/strand, before it exceeds the threshold, are reported."/> + </when> + <when value="no"> + <!-- Do nothing --> + </when> + </conditional> + </section> + <section name="scoring" expanded="false" title="Scoring"> + <param name="score_file" type="data" format="txt" optional="true" argument="--scores" label="Read the substitution scores and gap penalties (and possibly other options) from a scoring file (see help below for file fomat description)." help="This option cannot be used in conjunction with ‑‑match or inference."/> <!--TODO EDIT INFERENCE --> + <conditional name="match"> + <param name="match_selector" type="select" display="radio" label="Set the score values for a match and mismatch" argument="--match" help="Note that specifying ‑‑match changes the defaults for some of the other options (e.g. the scoring penalties for gaps, and various extension thresholds), as described in respective sections of LASTZ manual. The regular defaults are chosen for compatibility with BLASTZ, but since BLASTZ doesn't support ‑‑match, LASTZ infers that you are not expecting BLASTZ compatibility for this run, so it is free to use improved defaults. THIS OPTION CANNOT BE USED WITH --scores"> + <option value="yes">Yes</option> + <option value="no" selected="true">No</option> + </param> + <when value="yes"> + <param name="match_reward" type="integer" optional="true" label="Score value for a match"/> + <param name="match_penalty" type="integer" optional="true" label="Score value for a mismatch"/> + </when> + <when value="no"> + <!-- Do nothing --> + </when> + </conditional> + <conditional name="gap"> + <param name="gap_selector" type="select" display="radio" label="Set the score penalties for opening and extending a gap" argument="--gap" help="These are specified as positive values; subtraction is implicitly assumed. Note that the first base in a gap incurs the sum of both penalties. This option is only valid if gapped extension is being performed, and cannot be used in conjunction with inference. These values specified on the command line override any corresponding values from a file provided with ‑‑scores."> + <option value="yes">Yes</option> + <option value="no" selected="true">No</option> + </param> + <when value="yes"> + <param name="gap_open" type="integer" optional="true" label="Gap opening penalty"/> + <param name="gap_extend" type="integer" optional="true" label="Gap extension penalty"/> + </when> + <when value="no"> + <!-- Do nothing --> + </when> + </conditional> + <param name="ambigN" type="boolean" truevalue="--ambiguous=n" checked="false" label="Treat each N in the input sequences as an ambiguous nucleotide" argument="--ambiguous=n" help="Substitutions with N are scored as zero, instead of using the fill_score value from the scoring file (which is -100 by default)."/> + <param name="ambigIUPAC" type="boolean" truevalue="--ambiguous=iupac" checked="false" label="Treat each of the IUPAC-IUB ambiguity codes (B, D, H, K, M, R, S, V, W, and Y, as well as N) in the input sequences as a completely ambiguous nucleotide." argument="--ambiguous=iupac" help="Substitutions with these characters are scored as zero, instead of using the fill_score value from the scoring file (which is -100 by default)."/> + </section> + <section name="seeding" expanded="false" title="Seeding"> + <conditional name="seed"> + <param name="seed_selector" type="select" display="radio" label="Select seed type"> + <option value="defaults" selected="true">Use defaults</option> + <option value="pre_set">Use pre-set values</option> + <option value="len">Set length</option> + <option value="half_len">Set half length</option> + <option value="pattern">Set pattern</option> + </param> + <when value="defaults"> + <!-- Do nothing --> + </when> + <when value="pre_set"> + <param name="pre_set_seeds" type="select" display="radio" label="Select seed pattern" help="Select between 12of19 seed (19-bp word with matches in 12 specific positions: 1110100110010101111) or 14of22 seed (22-bp word with matches in 14 specific positions: 1110101100110010101111). 0 = mismatch, 1 = match" argument="--seed=12of19,--seed=14of22"> + <option value="--seed=12of19">12of19</option> + <option value="--seed=14of22">14of22</option> + </param> + </when> + <when value="len"> + <param name="seed_match" type="integer" optional="true" label="Set seed length" help="Seeds require a N-bp word with matches in all positions" argument="--seed=match" /> + </when> + <when value="half_len"> + <param name="seed_half" type="integer" optional="true" label="Set seed length" help="Seeds requires N-bp word with matches or transitions in all positions" argument="--seed=half" /> + </when> + <when value="pattern"> + <param name="pattern" type="text" size="25" optional="true" argument="--seed=PATTERN" label="Specify an arbitrary pattern" help="Use 1s, 0s, and Ts for seed discovery (e.g., 1110100110010101111 where 1 = match, 0 = mismatch, T = transition)" /> + </when> + </conditional> + <param name="transitions" type="select" display="radio" label="Allow transitions" help="In each seed, specify how many match positions can be transition(s) instead"> + <option value="--transition" selected="true">One</option> + <option value="--transition=2">Two</option> + <option value="--notransition">None</option> + </param> + <conditional name="seed_filer"> + <param name="seed_filer_selector" type="select" display="radio" label="Filter seeds" argument="--filter"> + <option value="yes">Yes</option> + <option value="no" selected="true">No</option> + </param> + <when value="yes"> + <param name="filter_tr" type="integer" optional="true" label="Number of transitions" help="Allowing no more than this number of transversions. If not specified, any number of transversions is allowed (they are not limited)" /> + <param name="filter_match" type="integer" optional="true" label="Number of matches" help="Require at least this many exact matches"/> + </when> + <when value="no"> + <!-- Do nothing --> + </when> + </conditional> + </section> + <section name="hsp" expanded="false" title="HSPs (Gap-free extension)"> + <param name="gfextend" type="boolean" truevalue="--gfextend" checked="false" argument="--gfextend" label="Perform gap-free extension of seeds to HSPs" help="This will take into account other papermeters in this section"/> + <param name="nogfextend" type="boolean" truevalue="--nogfextend" argument="--nogfextend" label="Skip the gap-free extension stage" help="Instead, pass the seeds along to the next specified stage.It is not recommended to use --nogfextend without also using --nogapped."/> + <conditional name="hsp_method"> + <param name="hsp_method_selector" type="select" display="radio" label="Select HSP finding method"> + <option value="none" selected="true">None</option> + <option value="match">Match extension</option> + <option value="mismatch">Mismatch extension</option> + <option value="x">X-drop extension</option> + </param> + <when value="none"> + <!-- Do nothing --> + </when> + <when value="match"> + <param name="exact" type="integer" optional="true" argument="--exact" label="Find HSPs using the exact match extension method with the given length threshold" help="This is instead of using the x-drop method"/> + </when> + <when value="mismatch"> + <param name="mismatch_count" type="integer" optional="true" label="Specify number of mismatches"/> + <param name="mismatch_length" type="integer" min="1" max="50" optional="true" label="Specify length threshold" help="Find HSPs using the mismatch extension method with the given length threshold and allowing specified number of mismatches" argument="--mismatch"/> + </when> + <when value="x"> + <param name="xdrop" type="integer" optional="true" argument="--xdrop" label="Find HSPs using the x-drop extension method with this threshold" help="The dropoff setting determines the endpoints of each gap-free segment: the extension of each seed is stopped when its cumulative score drops off by more than the given threshold from the maximum seen so far."/> + <param name="hspthresh" type="integer" optional="true" argument="--hspthresh" label="Set the score threshold for the x-drop extension method" help="HSPs scoring lower are discarded"/> + <param name="hspthresh_top" type="integer" optional="true" argument="--hspthresh=top" label="Set an adaptive score threshold for the x-drop extension method" help="HSPs scoring lower are discarded. The score threshold is chosen to limit the number of target sequence bases in HSPs to about this value (or possibly a little higher in case of ties, etc.)."/> + <param name="hspthresh_top_percent" type="integer" optional="true" argument="--hspthresh=top%" label="Set an adaptive score threshold for the x-drop extension method" help="HSPs scoring lower are discarded. The score threshold is chosen to limit the number of target sequence bases in HSPs to about this perentage value (or possibly a little higher in case of ties, etc.)."/> + </when> + </conditional> + <param name="entropy" type="boolean" truevalue="--entropy" checked="false" label="Adjust for entropy when qualifying HSPs in the x-drop extension method" help="Those that score just slightly above the HSP threshold are adjusted downward according to the entropy of their nucleotides, and any that then fall below the threshold are discarded."/> + <param name="entropy_report" type="boolean" truevalue="--entropy=report" checked="false" label="Adjust for entropy when qualifying HSPs in the x-drop extension method, and report (to stderr) any HSPs that are discarded as a result." help="Reported HSPs are printed to stderr"/> + <param name="noentropy" type="boolean" truevalue="--noentropy" checked="false" label="Don't adjust for entropy when qualifying HSPs"/> + </section> + <section name="chaining" expanded="false" title="Chaining"> + <param name="chain" type="boolean" truevalue="--chain" checked="false" argument="--chain" label="Perform chaining of HSPs with no penalties"/> + <conditional name="chaning_penalties"> + <param name="chaning_penalties_selector" type="select" display="radio" argument="--chain=" label="Perform chaining with penalties"> + <option value="yes">Yes</option> + <option value="no" selected="true">No</option> + </param> + <when value="yes"> + <param name="diag" type="integer" optional="true" label="Penalty for diagonal in dynamic programming matrix"/> + <param name="anti" type="integer" optional="true" label="Penalty for anti-diagonal in dynamic programming matrix" help="These are specified as positive values; subtraction from the score is implicitly assumed."/> + </when> + <when value="no"> + <!-- Do nothing --> + </when> + </conditional> + </section> + <section name="gap_ext" expanded="false" title="Gapped extension"> + <param name="gapped" type="boolean" truevalue="--gapped" argument="--gapped" label="Perform gapped extension of HSPs" help="Extension of HSPs (or seeds, if gap-free extension is not performed), is performed after first reducing them to anchor points."/> + <param name="nogapped" type="boolean" truevalue="--nogapped" argument="--nogapped" label="Skip the gapped extension stage" help="This means that interpolation must also be skipped, since it is not allowed without gapped extension"/> + <param name="ydrop" type="integer" optional="true" argument="--ydrop" label="Set the threshold for terminating gapped extension" help="This restricts the endpoints of each local alignment by limiting the local region around each anchor in which extension is performed. The boundary of this region in the dynamic programming matrix is formed by the points where the cumulative score has dropped off by more than the given threshold from the maximum seen so far."/> + <param name="noytrim" type="boolean" truevalue="--noytrim" argument="--noytrim" label="If y-drop extension encounters the end of the sequence, extend the alignment to the end of the sequence rather than trimming it back to the location giving the maximum score" help="This is highly recommended when either the target or query sequences are short reads (say, less than 100 bases), to prevent y-drop mismatch shadow."/> + <param name="gappedthresh" type="integer" optional="true" argument="--gappedthresh" label="Set the threshold for gapped extension" help="Alignments scoring lower than that value are discarded. When used along with the x-drop method for gap-free extension, this value is generally set at least as high as the HSP threshold. Setting it lower has no effect, since at worst the HSP itself would always qualify (both extension stages use the same scoring matrix)."/> + <param name="allgappedbounds" type="boolean" truevalue="--allgappedbounds" argument="--allgappedbounds" label="Revert to handling bounding alignments the way they were handled in BLASTZ."/> + </section> + <section name="filters" expanded="false" title="Filtering"> + <section name="identity" expanded="true" title="Filter alignments by percent identity"> + <param name="id_min" type="integer" min="0" max="100" optional="true" label="Minimum identity"/> + <param name="id_max" type="integer" min="0" max="100" optional="true" argument="--filter=identity" label="Maximum identity (optional)" help="0 ≤ min ≤ max ≤ 100 percent. Identity is the percentage of aligned bases that are matches. Alignment blocks outside the given range are discarded."/> + </section> + <section name="continuity" expanded="false" title="Filter alignments by continuity"> + <param name="cont_min" type="integer" min="0" max="100" optional="true" label="Minimum continuity"/> + <param name="cont_max" type="integer" min="0" max="100" optional="true" argument="--filter=continuity" label="Maximum continuity (optional)" help="0 ≤ min ≤ max ≤ 100 percent. Continuity is the percentage of alignment columns that are not gaps. Alignment blocks outside the given range are discarded."/> + </section> + <section name="coverage" expanded="false" title="Filter alignments by coverage"> + <param name="cov_min" type="integer" min="0" max="100" optional="true" label="Minimum coverage"/> + <param name="cov_max" type="integer" min="0" max="100" optional="true" argument="--filter=coverage" label="Maximum coverage (optional)" help=" 0 ≤ min ≤ max ≤ 100 percent. Coverage is the percentage of the entire target or query sequence (whichever is shorter) that is included in the alignment block. Blocks outside the given range are discarded."/> + </section> + <param name="filter_nmatch" type="integer" min="1" optional="true" argument="--filter=nmatch" label="Filter alignments by how many bases match" help="Requiring at least this number of matched bases, min > 0. Match count is the number of matched bases in the alignment."/> + <param name="filter_nmatch_percent" type="integer" min="1" optional="true" argument="--filter=nmatch%" label="Filter alignments by how many bases match expressed as percentage" help="e.g., percentage of the query length."/> + <param name="filter_nmismatch" type="integer" min="0" optional="true" argument="--filter=nmismatch:0.." label="Filter alignments by the number of mismatches" help="Allow no more than this number of mismatched bases. Mismatch count, or nmismatch, is the number of aligned bases in the alignment that are mismatches (substitutions)."/> + <param name="filter_ngap" type="integer" min="0" optional="true" argument="--filter=ngap:0.." label="Filter alignments by the number of gaps" help="Allow no more than this number of gaps. Gap count, or ngap, is the number of runs of gapped columns in the alignment (each run is counted as one gap)."/> + <param name="filter_cgap" type="integer" min="0" optional="true" argument="--filter=cgap:0.." label="Filter alignments by the number of gap columns" help="Allow no more than this number of gaps. Gap column count, or cgap, is the number of gapped columns in the alignment (each column is counted as one gap)."/> + <param name="notrivial" type="boolean" truevalue="--notrivial" argument="--notrivial" label="Do not output a trivial self-alignment block if the target and query sequences are identical" help="Note that using ‑‑self automatically enables this option."/> + </section> + <section name="interpolation" expanded="false" title="Interpolation"> + <param name="inner" type="integer" optional="true" argument="--inner" label="Perform additional alignment between the gapped alignment blocks, using (presumably) more sensitive alignment parameters" help="This value is used as the threshold for both the gap-free and gapped extension sub-stages; see the discussion of interpolation for more details. This option is only valid if gapped extension is performed."/> + </section> + <section name="output_format" expanded="false" title="Output"> + <conditional name="out"> + <param name="format" type="select" display="radio" label="Specify the output format"> + <option value="bam" selected="true">BAM --format=sam)</option> + <option value="general_def">General default (--format=general)</option> + <option value="general_full">Customized general (‑‑format=general[:fields])</option> + <option value="maf">MAF (--format=maf)</option> + <option value="blastn">blastn (--format=BLASTN)</option> + </param> + <when value="bam"> + <param name="bam_options" type="select" display="radio" argument="--format=sam, --format=softsam" label="Select a BAM flavor to output" help="Lastz actually outputs SAM data but Galaxy converts it into BAM to save space. For alignments that don't reach the end of a query, ‑‑format=sam uses 'hard clipping', while ‑‑format=softsam uses 'soft clipping'. See the section on 'clipped alignment' in the SAM specification for an explanation of what this means. The options ‑‑format=sam- and ‑‑format=softsam- suppress the SAM header lines. This makes them suitable for concatenating output from multiple runs. If you need to specify readgroup information: use AddOrEplaceReadGroups from Picard package"> + <option value="sam" selected="true">BAM</option> + <option value="softsam">soft-clipped BAM</option> + <option value="sam-">BAM without header</option> + <option value="softsam-">soft-clipped BAM without header</option> + </param> + </when> + <when value="general_def"> + <!-- Do nothing --> + </when> + <when value="general_full"> + <param name="fields" type="select" display="checkboxes" multiple="true" label="Select which fields to include" argument="--format=general-[:fields]"> + <option value="score" selected="true">score: Score of the alignment block</option> + <option value="name1" selected="true">name1: Name of the target sequence</option> + <option value="number1">number1: Number of the target sequence within the target file</option> + <option value="strand1" selected="true">strand1: Target sequence strand </option> + <option value="size1" selected="true">size1: Size of the entire target sequence</option> + <option value="start1">start1: Starting position of the alignment block in the target, origin-one</option> + <option value="zstart1" selected="true">zstart1: Starting position of the alignment block in the target, origin-zero</option> + <option value="end1" selected="true">end1: Ending position of the alignment block in the target</option> + <option value="length1">length1: Length of the alignment block in the target (excluding gaps)</option> + <option value="text1">text1: Aligned characters in the target, including gap characters</option> + <option value="qalign1">qalign1: The target quality sequence (if there is one) correpsonding to aligned characters</option> + <option value="nucs1">nucs1: The entire target sequence</option> + <option value="name2" selected="true">name2: Name of the query sequence</option> + <option value="number2">number2: Number of the query sequence within the query file</option> + <option value="strand2" selected="true">strand2: Query sequence strand</option> + <option value="size2" selected="true">size2: Size of the entire query sequence</option> + <option value="start2">start2: Starting position of the alignment block in the query, origin-one</option> + <option value="zstart2" selected="true">zstart2: Starting position of the alignment block in the query, origin-one</option> + <option value="end2" selected="true">end2: Ending position of the alignment block in the query</option> + <option value="length2">length2: Length of the alignment block in the query (excluding gaps)</option> + <option value="text2">text2: Aligned characters in the query, including gap characters</option> + <option value="qalign2">qalign2: The query quality sequence (if there is one) correpsonding to aligned characters</option> + <option value="nucs2">nucs2: The entire query sequence</option> + <option value="nmatch">nmatch: Match count</option> + <option value="nmismatch">nmismatch: Mismatch count</option> + <option value="ncolumn">ncolumn: Number of columns in the block. This includes matches, mismatches (substitutions), and gaps</option> + <option value="npair">npair: Number of aligned bases in the block that are matches or mismatches (substitutions)</option> + <option value="ngap">ngap: Gap count, the number of gaps in the block, counting each run of gapped columns as a single gap</option> + <option value="cgap">cgap: Gap column count, the number of gaps in the block, counting each gapped column as a separate gap</option> + <option value="diff">diff: Differences between what would be written for text1 and text2</option> + <option value="cigar">cigar: A CIGAR-like representation of the alignment’s path</option> + <option value="cigarx">cigarx: Same as cigar, but uses a newer syntax that distinguishes matches from substitutions</option> + <option value="identity" selected="true">identity: Fraction of aligned bases in the block that are matches </option> + <option value="idfrac">idfrac: Fraction of aligned bases in the block that are matches </option> + <option value="id%" selected="true">id% Fraction of aligned bases in the block that are matches (as %)</option> + <option value="blastid%">blastid%: Fraction of the alignment block that is matches, as would be reported by NCBI BLAST</option> + <option value="continuity">continuity: Rate of non-gaps (non-indels) in the alignment block</option> + <option value="confrac">confrac: Rate of non-gaps (non-indels) in the alignment block (as fraction)</option> + <option value="con%">con%: Rate of non-gaps (non-indels) in the alignment block (as %)</option> + <option value="coverage" selected="true">coverage: Fraction of the entire input sequence (target or query, whichever is shorter) that is covered by the alignment block</option> + <option value="covfrac">covfrac: Fraction of the entire input sequence (target or query, whichever is shorter) that is covered by the alignment block (as fraction)</option> + <option value="cov%" selected="true">cov%: Fraction of the entire input sequence (target or query, whichever is shorter) that is covered by the alignment block (as %)</option> + <option value="diagonal">diagonal: The diagonal of the start of the alignment block in the DP matrix, expressed as an identifying number start1-start2</option> + <option value="shingle">shingle: A measurement of the shingle overlap between the target and the query</option> + <option value="number">number: The alignment number, counted as alignments are written to output (1-base)</option> + <option value="znumber">znumber: The alignment number, counted as alignments are written to output (0-base)</option> + <sanitizer invalid_char=""> + <valid initial="string.letters,string.digits"><add value="%" /> </valid> + </sanitizer> + </param> + </when> + <when value="maf"> + <param name="maf_type" type="select" display="radio" argument="--format=maf" label="Seleat MAF flavor" help="MAF is a multiple alignment format developed at UCSC"> + <option value="maf">MAF</option> + <option value="maf+">MAF with additional stats</option> + <option value="maf-">MAF without header and comments</option> + </param> + </when> + <when value="blastn"> + <!-- Do nothing --> + </when> + </conditional> + </section> + </inputs> + <outputs> + <data format="tabular" name="output" label="${tool.name} on ${on_string}: mapped reads"> + <change_format> + <when input="output_format.out.format" value="bam" format="bam" /> + <when input="output_format.out.format" value="maf" format="maf" /> + </change_format> + </data> + <data format="png" name="out_plot" label="${tool.name} on ${on_string}: dot plot"/> + </outputs> + <tests> + <test> + <param name="ref_source" value="cached" /> + <param name="target_2bit" value="phiX174" /> + <param name="query" value="phiX_split.fasta" /> + <param name="strand" value="--strand=both" /> + <param name="format" value="general_def" /> + <output name="output" value="test1.out" /> + </test> + <test> + <param name="ref_source" value="history" /> + <param name="target" value="phiX_split.fasta" /> + <param name="query" value="phiX.fasta" /> + <param name="strand" value="--strand=both" /> + <param name="format" value="general_def" /> + <output name="output" value="test2.out" /> + </test> + <test> + <param name="ref_source" value="history" /> + <param name="target" value="phiX_split.fasta" /> + <param name="query" value="phiX.fasta" /> + <param name="strand" value="--strand=both" /> + <param name="score_file" value="score_file.txt" /> + <param name="format" value="general_def" /> + <output name="output" value="test3.out" /> + </test> + </tests> + + <help><![CDATA[ + + **What is does** + + LASTZ is designed to preprocess one sequence or set of sequences (which we collectively call the *TARGET*) and then align several *QUERY* sequences to it. It was developed by `Bob Harris <http://www.bx.psu.edu/~rsharris/>`_ in the lab of Webb Miller at Penn State. + + .. class:: warningmark + + **Read documentation** before proceeding. LASTZ is a complex tool with many parameter options. Fortunately, there is a `great manual <https://lastz.github.io/lastz/>`_ maintained by its author. Default parameters may be sufficient to obtain the initial idea about how similar your sequences are, but to produce reliable alignments you may need to tweak the parameters. So RTFM! + + **About LASTZ parameters** + + Galaxy's version of LASTZ has nine parameter sections (*Where to look*, *Scoring*, *Seeding*, *HSPs*, *Chaining*, *Gapped extension*, *Filtering*, *Interpolation*, and *Output*). These sections closely follow parameter description in the `manual <https://lastz.github.io/lastz/#syntax>`_. + + **Defaults** + + here are defaults for some of the most important parameters:: + + --seed=<pattern> set seed pattern (12of19, 14of22, or general pattern) + (default is 1110100110010101111) + SEE "Seeding" SECTION -> "Select seed type" + + --[no]transition allow (or don't) one transition in a seed hit + (by default a transition is allowed) + SEE "Seeding" SECTION -> "Allow transitions" + + --[no]chain perform chaining + (by default no chaining is performed) + SEE "Chaining" SECTION + + --[no]gapped perform gapped alignment (instead of gap-free) + (by default gapped alignment is performed) + SEE "Gapped extension" SECTION + + --strand=both search both strands + --strand=plus search + strand only (matching strand of query spec) + (by default both strands are searched) + SEE "Where to look" SECTION + + --scores=<file> read substitution and gap scores from a file + SEE "Scoring" SECTION + + --xdrop=<score> set x-drop threshold (default is 10sub[A][A]) + SEE "HSPs" SECTION + + --ydrop=<score> set y-drop threshold (default is open+300extend) + SEE "Gapped extension" SECTION + + --hspthresh=<score> set threshold for high scoring pairs (default is 3000) + ungapped extensions scoring lower are discarded + <score> can also be a percentage or base count + SEE "HSPs" SECTION + + --gappedthresh=<score> set threshold for gapped alignments + gapped extensions scoring lower are discarded + <score> can also be a percentage or base count + (default is to use same value as --hspthresh) + SEE "Gapped extension" SECTION + + + **Substitution matrix** + + By default the HOXD70 substitution scores are used (from `Chiaromonte et al. 2002 <https://www.ncbi.nlm.nih.gov/pubmed/11928468>`_):: + + bad_score = X:-1000 # used for sub['X'][*] and sub[*]['X'] + fill_score = -100 # used when sub[*][*] is not defined + gap_open_penalty = 400 + gap_extend_penalty = 30 + + A C G T + A 91 -114 -31 -123 + C -114 100 -125 -31 + G -31 -125 100 -114 + T -123 -31 -114 91 + + Matrix can be supplied as an input to **Read the substitution scores** parameter in *Scoring* section. Substitution matrix can be inferred from your data using another LASTZ-based tool (LASTZ_D: Infer substitution scores). + + **Output** + + This version of LASTZ produces two outputs by default: a BAM alignment file and a dot-plot in PNG format. Other formats can be configured in *Output* section. This incarnation of LASTZ produces outputs without comment line starting with '#'. To learn identity of each column, consult `formats section of LASTZ manual <https://lastz.github.io/lastz/#formats>`_. + + ]]> + </help> + <expand macro="citations"/> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/lastz_d.xml Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,82 @@ +<tool id="lastz_d_wrapper" name="LASTZ_D" version="1.3"> + <description>: estimate substitution scores matrix</description> + <macros> + <import>lastz_macros.xml</import> + </macros> + <requirements> + <requirement type="package" version="@LASTZ_CONDA_VERSION@">lastz</requirement> + </requirements> + <command detect_errors="exit_code"><![CDATA[ + lastz_D + @TARGET_INPUT_COMMAND_LINE@ + '${query}' + #if $score_file: + '--inferonly=${score_file}' + #else: + --inferonly + #end if + '--infscores=${output}' + ]]> + </command> + <inputs> + <expand macro="target_input"/> + <param name="query" format="fasta,fastq" type="data" label="Select QUERY sequence(s)" help="These are the sequences that you are aligning against TARGET"/> + <param name="score_file" type="data" format="txt" optional="true" label="Control file for inference" argument="--inferonly[=control_file]" help="Optional controf file. If nothing is selected, LASTZ_D uses default described in the manual"/> + </inputs> + <outputs> + <data format="txt" name="output" label="${tool.name} on ${on_string}: substituion matrix"/> + </outputs> + <tests> + <test> + <param name="ref_source" value="history" /> + <param name="target" value="chrM_human.fa" /> + <param name="query" value="chrM_mouse.fa" /> + <output name="output" value="lastz_d_test1.out" /> + </test> + <test> + <param name="ref_source" value="history" /> + <param name="target" value="chrM_human.fa" /> + <param name="query" value="chrM_mouse.fa" /> + <param name="score_file" value="lastz_d_ctrl_file.txt" /> + <output name="output" value="lastz_d_test2.out" /> + </test> + </tests> + + <help><![CDATA[ + + **What is does** + + LASTZ_D is a non-integer (**D** stands for Double) version of LASTZ that can be used to estimate substitution matrix that will be used to score alignments. It was developed by `Bob Harris <http://www.bx.psu.edu/~rsharris/>`_ in the lab of Webb Miller at Penn State as a part of LASTZ. Matrix computed by this tool is to be used by LASTZ (see below). + + .. class:: warningmark + + **Read documentation** before proceeding. LASTZ is a complex tool with many parameter options. Fortunately, there is a `great manual <https://lastz.github.io/lastz/>`_ maintained by its author. The two sections that are particularly relevant to the inference of substitution matrix are `Inferring Score Sets <http://www.bx.psu.edu/~rsharris/lastz/README.lastz-1.04.00.html#adv_inference>`_ and `Inference Control File <http://www.bx.psu.edu/~rsharris/lastz/README.lastz-1.04.00.html#fmt_inference>`_. + + **Notes on the inference** + + Inference is achieved by computing the probability of each of the 18 different alignment events (gap open, gap extend, and 16 substitutions). These probabilities are estimated from alignments of the sequences. Of course, at first we don't have alignments, so the process begins by using a generic scoring set to create alignments, infer scores from those, then realign, and so on, until the scores stabilize or "converge". Ungapped alignments are performed until the substitution scores converge, then gapped alignments are performed (holding the substitution scores constant) until the gap penalties converge. In the end you get a matrix like this:: + + # (a LASTZ scoring set, created by "LASTZ --infer") + + bad_score = X:-1781 # used for sub[X][*] and sub[*][X] + fill_score = -178 # used when sub[*][*] not otherwise defined + gap_open_penalty = 400 + gap_extend_penalty = 30 + + A C G T + A 72 -79 -49 -97 + C -79 100 -178 -49 + G -49 -178 100 -79 + T -97 -49 -79 72 + + This dataset can then be used as an input to the **Read the substitution scores** parameter of LASTZ (Parameter section *Scoring*). + + The iterative process can fail if there's not a lot of sequence to align. E.g. if after the 4th iteration there's nothing in the central 50% denominators go to zero and the process fails. + + If the sequences you are aligning have GC content different than the usual ACGT 30-20-20-30 split, scoring inference should discover this and give you better alignments. + + + ]]> + </help> + <expand macro="citations"/> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/lastz_macros.xml Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,41 @@ +<macros> + <token name="@LASTZ_CONDA_VERSION@">1.0.4</token> + <token name="@TARGET_INPUT_COMMAND_LINE@"> + #if $source.ref_source=="history": + '${source.target}' + #else: + '${source.target_2bit.fields.path}' + #end if + </token> + <xml name="target_input"> + <conditional name="source"> + <param name="ref_source" type="select" label="Select TARGET sequnce(s) to align against" help="If your TARGET is in history, choose 'from your history' option"> + <option value="cached">locally cached</option> + <option value="history">from your history</option> + </param> + <when value="cached"> + <param name="target_2bit" type="select" label="Using reference genome" help="If your genome of interest is not listed, contact the Galaxy team"> + <options from_data_table="lastz_seqs" /> + </param> + </when> + <when value="history"> + <param name="target" type="data" format="fasta" label="Select a reference dataset" /> + </when> + </conditional> + </xml> + <xml name="citations"> + <citations> + <citation type="bibtex"> + @misc{ + githublastz, + author = {Harris, Robert}, + year = {2007}, + title = {Improved pairwise alignment of genomic DNA}, + publisher = {The Pennsylvania State University}, + journal = {Ph. D. Thesis}, + url = {http://www.bx.psu.edu/~rsharris/rsharris_phd_thesis_2007.pdf}, + } + </citation> + </citations> + </xml> +</macros>
--- a/lastz_wrapper.py Tue Oct 13 12:23:37 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,290 +0,0 @@ -#!/usr/bin/env python - -""" -Runs Lastz -Written for Lastz v. 1.01.88. - -usage: lastz_wrapper.py [options] - --ref_name: The reference name to change all output matches to - --ref_source: Whether the reference is cached or from the history - --source_select: Whether to used pre-set or cached reference file - --input1: The name of the reference file if using history or reference base name if using cached - --input2: The reads file to align - --ref_sequences: The number of sequences in the reference file if using one from history - --pre_set_options: Which of the pre set options to use, if using pre-sets - --strand: Which strand of the read to search, if specifying all parameters - --seed: Seeding settings, if specifying all parameters - --gfextend: Whether to perform gap-free extension of seed hits to HSPs (high scoring segment pairs), if specifying all parameters - --chain: Whether to perform chaining of HSPs, if specifying all parameters - --transition: Number of transitions to allow in each seed hit, if specifying all parameters - --O: Gap opening penalty, if specifying all parameters - --E: Gap extension penalty, if specifying all parameters - --X: X-drop threshold, if specifying all parameters - --Y: Y-drop threshold, if specifying all parameters - --K: Threshold for HSPs, if specifying all parameters - --L: Threshold for gapped alignments, if specifying all parameters - --entropy: Whether to involve entropy when filtering HSPs, if specifying all parameters - --identity_min: Minimum identity (don't report matches under this identity) - --identity_max: Maximum identity (don't report matches above this identity) - --coverage: The minimum coverage value (don't report matches covering less than this) - --unmask: Whether to convert lowercase bases to uppercase - --out_format: The format of the output file (sam, diffs, or tabular (general)) - --output: The name of the output file - --lastzSeqsFileDir: Directory of local lastz_seqs.loc file -""" -import optparse, os, subprocess, shutil, sys, tempfile, threading, time -from Queue import Queue - -from galaxy import eggs -import pkg_resources -pkg_resources.require( 'bx-python' ) -from bx.seq.twobit import * -from bx.seq.fasta import FastaReader -from galaxy.util.bunch import Bunch - -STOP_SIGNAL = object() -WORKERS = 4 -SLOTS = 128 - -def stop_err( msg ): - sys.stderr.write( "%s" % msg ) - sys.exit() - -def stop_queues( lastz, combine_data ): - # This method should only be called if an error has been encountered. - # Send STOP_SIGNAL to all worker threads - for t in lastz.threads: - lastz.put( STOP_SIGNAL, True ) - combine_data.put( STOP_SIGNAL, True ) - -class BaseQueue( object ): - def __init__( self, num_threads, slots=-1 ): - # Initialize the queue and worker threads - self.queue = Queue( slots ) - self.threads = [] - for i in range( num_threads ): - worker = threading.Thread( target=self.run_next ) - worker.start() - self.threads.append( worker ) - def run_next( self ): - # Run the next job, waiting until one is available if necessary - while True: - job = self.queue.get() - if job is STOP_SIGNAL: - return self.shutdown() - self.run_job( job ) - time.sleep( 1 ) - def run_job( self, job ): - stop_err( 'Not Implemented' ) - def put( self, job, block=False ): - # Add a job to the queue - self.queue.put( job, block ) - def shutdown( self ): - return - -class LastzJobQueue( BaseQueue ): - """ - A queue that runs commands in parallel. Blocking is done so the queue will - not consume much memory. - """ - def run_job( self, job ): - # Execute the job's command - proc = subprocess.Popen( args=job.command, shell=True, stderr=subprocess.PIPE, ) - proc.wait() - stderr = proc.stderr.read() - proc.wait() - if stderr: - stop_queues( self, job.combine_data_queue ) - stop_err( stderr ) - job.combine_data_queue.put( job ) - -class CombineDataQueue( BaseQueue ): - """ - A queue that concatenates files in serial. Blocking is not done since this - queue is not expected to grow larger than the command queue. - """ - def __init__( self, output_filename, num_threads=1 ): - BaseQueue.__init__( self, num_threads ) - self.CHUNK_SIZE = 2**20 # 1Mb - self.output_file = open( output_filename, 'wb' ) - def run_job( self, job ): - in_file = open( job.output, 'rb' ) - while True: - chunk = in_file.read( self.CHUNK_SIZE ) - if not chunk: - in_file.close() - break - self.output_file.write( chunk ) - for file_name in job.cleanup: - os.remove( file_name ) - def shutdown( self ): - self.output_file.close() - return - -def __main__(): - #Parse Command Line - parser = optparse.OptionParser() - parser.add_option( '', '--ref_name', dest='ref_name', help='The reference name to change all output matches to' ) - parser.add_option( '', '--ref_source', dest='ref_source', help='Whether the reference is cached or from the history' ) - parser.add_option( '', '--ref_sequences', dest='ref_sequences', help='Number of sequences in the reference dataset' ) - parser.add_option( '', '--source_select', dest='source_select', help='Whether to used pre-set or cached reference file' ) - parser.add_option( '', '--input1', dest='input1', help='The name of the reference file if using history or reference base name if using cached' ) - parser.add_option( '', '--input2', dest='input2', help='The reads file to align' ) - parser.add_option( '', '--pre_set_options', dest='pre_set_options', help='Which of the pre set options to use, if using pre-sets' ) - parser.add_option( '', '--strand', dest='strand', help='Which strand of the read to search, if specifying all parameters' ) - parser.add_option( '', '--seed', dest='seed', help='Seeding settings, if specifying all parameters' ) - parser.add_option( '', '--transition', dest='transition', help='Number of transitions to allow in each seed hit, if specifying all parameters' ) - parser.add_option( '', '--gfextend', dest='gfextend', help='Whether to perform gap-free extension of seed hits to HSPs (high scoring segment pairs), if specifying all parameters' ) - parser.add_option( '', '--chain', dest='chain', help='Whether to perform chaining of HSPs, if specifying all parameters' ) - parser.add_option( '', '--O', dest='O', help='Gap opening penalty, if specifying all parameters' ) - parser.add_option( '', '--E', dest='E', help='Gap extension penalty, if specifying all parameters' ) - parser.add_option( '', '--X', dest='X', help='X-drop threshold, if specifying all parameters' ) - parser.add_option( '', '--Y', dest='Y', help='Y-drop threshold, if specifying all parameters' ) - parser.add_option( '', '--K', dest='K', help='Threshold for HSPs, if specifying all parameters' ) - parser.add_option( '', '--L', dest='L', help='Threshold for gapped alignments, if specifying all parameters' ) - parser.add_option( '', '--entropy', dest='entropy', help='Whether to involve entropy when filtering HSPs, if specifying all parameters' ) - parser.add_option( '', '--identity_min', dest='identity_min', help="Minimum identity (don't report matches under this identity)" ) - parser.add_option( '', '--identity_max', dest='identity_max', help="Maximum identity (don't report matches above this identity)" ) - parser.add_option( '', '--coverage', dest='coverage', help="The minimum coverage value (don't report matches covering less than this)" ) - parser.add_option( '', '--unmask', dest='unmask', help='Whether to convert lowercase bases to uppercase' ) - parser.add_option( '', '--out_format', dest='format', help='The format of the output file (sam, diffs, or tabular (general))' ) - parser.add_option( '', '--output', dest='output', help='The output file' ) - parser.add_option( '', '--lastzSeqsFileDir', dest='lastzSeqsFileDir', help='Directory of local lastz_seqs.loc file' ) - ( options, args ) = parser.parse_args() - - # output version # of tool - try: - tmp = tempfile.NamedTemporaryFile().name - tmp_stdout = open( tmp, 'wb' ) - proc = subprocess.Popen( args='lastz -v', shell=True, stdout=tmp_stdout ) - tmp_stdout.close() - returncode = proc.wait() - stdout = None - for line in open( tmp_stdout.name, 'rb' ): - if line.lower().find( 'version' ) >= 0: - stdout = line.strip() - break - if stdout: - sys.stdout.write( '%s\n' % stdout ) - else: - raise Exception - except: - sys.stdout.write( 'Could not determine Lastz version\n' ) - - if options.unmask == 'yes': - unmask = '[unmask]' - else: - unmask = '' - if options.ref_name: - ref_name = '[nickname=%s]' % options.ref_name - else: - ref_name = '' - # Prepare for commonly-used preset options - if options.source_select == 'pre_set': - set_options = '--%s' % options.pre_set_options - # Prepare for user-specified options - else: - set_options = '--%s --%s --gapped --strand=%s --seed=%s --%s O=%s E=%s X=%s Y=%s K=%s L=%s --%s' % \ - ( options.gfextend, options.chain, options.strand, options.seed, options.transition, - options.O, options.E, options.X, options.Y, options.K, options.L, options.entropy ) - # Specify input2 and add [fullnames] modifier if output format is diffs - if options.format == 'diffs': - input2 = '%s[fullnames]' % options.input2 - else: - input2 = options.input2 - if options.format == 'tabular': - # Change output format to general if it's tabular and add field names for tabular output - format = 'general-' - tabular_fields = ':score,name1,strand1,size1,start1,zstart1,end1,length1,text1,name2,strand2,size2,start2,zstart2,end2,start2+,zstart2+,end2+,length2,text2,diff,cigar,identity,coverage,gaprate,diagonal,shingle' - elif options.format == 'sam': - # We currently ALWAYS suppress SAM headers. - format = 'sam-' - tabular_fields = '' - else: - format = options.format - tabular_fields = '' - - # Set up our queues - lastz_job_queue = LastzJobQueue( WORKERS, slots=SLOTS ) - combine_data_queue = CombineDataQueue( options.output ) - - if options.ref_source == 'history': - # Reference is a fasta dataset from the history, so split job across - # the number of sequences in the dataset ( this could be a HUGE number ) - try: - # Ensure there is at least 1 sequence in the dataset ( this may not be necessary ). - error_msg = "The reference dataset is missing metadata, click the pencil icon in the history item and 'auto-detect' the metadata attributes." - ref_sequences = int( options.ref_sequences ) - if ref_sequences < 1: - stop_queues( lastz_job_queue, combine_data_queue ) - stop_err( error_msg ) - except: - stop_queues( lastz_job_queue, combine_data_queue ) - stop_err( error_msg ) - seqs = 0 - fasta_reader = FastaReader( open( options.input1 ) ) - while True: - # Read the next sequence from the reference dataset - seq = fasta_reader.next() - if not seq: - break - seqs += 1 - # Create a temporary file to contain the current sequence as input to lastz - tmp_in_fd, tmp_in_name = tempfile.mkstemp( suffix='.in' ) - tmp_in = os.fdopen( tmp_in_fd, 'wb' ) - # Write the current sequence to the temporary input file - tmp_in.write( '>%s\n%s\n' % ( seq.name, seq.text ) ) - tmp_in.close() - # Create a 2nd temporary file to contain the output from lastz execution on the current sequence - tmp_out_fd, tmp_out_name = tempfile.mkstemp( suffix='.out' ) - os.close( tmp_out_fd ) - # Generate the command line for calling lastz on the current sequence - command = 'lastz %s%s%s %s %s --ambiguousn --nolaj --identity=%s..%s --coverage=%s --format=%s%s > %s' % \ - ( tmp_in_name, unmask, ref_name, input2, set_options, options.identity_min, - options.identity_max, options.coverage, format, tabular_fields, tmp_out_name ) - # Create a job object - job = Bunch() - job.command = command - job.output = tmp_out_name - job.cleanup = [ tmp_in_name, tmp_out_name ] - job.combine_data_queue = combine_data_queue - # Add another job to the lastz_job_queue. Execution - # will wait at this point if the queue is full. - lastz_job_queue.put( job, block=True ) - # Make sure the value of sequences in the metadata is the same as the - # number of sequences read from the dataset ( this may not be necessary ). - if ref_sequences != seqs: - stop_queues( lastz_job_queue, combine_data_queue ) - stop_err( "The value of metadata.sequences (%d) differs from the number of sequences read from the reference (%d)." % ( ref_sequences, seqs ) ) - else: - # Reference is a locally cached 2bit file, split job across number of chroms in 2bit file - tbf = TwoBitFile( open( options.input1, 'r' ) ) - for chrom in tbf.keys(): - # Create a temporary file to contain the output from lastz execution on the current chrom - tmp_out_fd, tmp_out_name = tempfile.mkstemp( suffix='.out' ) - os.close( tmp_out_fd ) - command = 'lastz %s/%s%s%s %s %s --ambiguousn --nolaj --identity=%s..%s --coverage=%s --format=%s%s >> %s' % \ - ( options.input1, chrom, unmask, ref_name, input2, set_options, options.identity_min, - options.identity_max, options.coverage, format, tabular_fields, tmp_out_name ) - # Create a job object - job = Bunch() - job.command = command - job.output = tmp_out_name - job.cleanup = [ tmp_out_name ] - job.combine_data_queue = combine_data_queue - # Add another job to the lastz_job_queue. Execution - # will wait at this point if the queue is full. - lastz_job_queue.put( job, block=True ) - - # Stop the lastz_job_queue - for t in lastz_job_queue.threads: - lastz_job_queue.put( STOP_SIGNAL, True ) - # Although all jobs are submitted to the queue, we can't shut down the combine_data_queue - # until we know that all jobs have been submitted to its queue. We do this by checking - # whether all of the threads in the lastz_job_queue have terminated. - while threading.activeCount() > 2: - time.sleep( 1 ) - # Now it's safe to stop the combine_data_queue - combine_data_queue.put( STOP_SIGNAL ) - -if __name__=="__main__": __main__()
--- a/lastz_wrapper.xml Tue Oct 13 12:23:37 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,523 +0,0 @@ -<tool id="lastz_wrapper_2" name="Lastz" version="1.2.2"> - <requirements> - <requirement type="package" version="1.02.00">lastz</requirement> - </requirements> - <description> map short reads against reference sequence</description> - <command interpreter="python">lastz_wrapper.py - #if $seq_name.how_to_name=="yes": - --ref_name="${seq_name.ref_name}" - #end if - --ref_source="${source.ref_source}" - --source_select="${params.source_select}" - --out_format="${out_format}" - --input2="${input2}" - #if $source.ref_source=="history": - --input1="${source.input1}" - --ref_sequences="${input1.metadata.sequences}" - #else: - --input1="${source.input1_2bit.fields.path}" - --ref_sequences="None" - #end if - #if $params.source_select=="pre_set": - --pre_set_options="${params.pre_set_options}" - #else: - --strand="${params.strand}" - --seed="${params.seed}" - --gfextend="${params.gfextend}" - --chain="${params.chain}" - --transition="${params.transition}" - --O="${params.O}" - --E="${params.E}" - --X="${params.X}" - --Y="${params.Y}" - --K="${params.K}" - --L="${params.L}" - --entropy="${params.entropy}" - #end if - --identity_min="${min_ident}" - --identity_max="${max_ident}" - --coverage="${min_cvrg}" - --output="${output1}" - --unmask="${unmask}" - --lastzSeqsFileDir="${GALAXY_DATA_INDEX_DIR}" - </command> - <inputs> - <param name="input2" format="fasta" type="data" label="Align sequencing reads in" /> - <conditional name="source"> - <param name="ref_source" type="select" label="Against reference sequences that are"> - <option value="cached">locally cached</option> - <option value="history">in your history</option> - </param> - <when value="cached"> - <param name="input1_2bit" type="select" label="Using reference genome" help="If your genome of interest is not listed, contact the Galaxy team"> - <options from_data_table="lastz_seqs" /> - </param> - </when> - <when value="history"> - <param name="input1" type="data" format="fasta" label="Select a reference dataset" /> - </when> - </conditional> - <param name="out_format" type="select" label="Output format"> - <option value="sam">SAM</option> - <option value="diffs">Polymorphisms</option> - <option value="tabular">Tabular</option> - </param> - <conditional name="params"> - <param name="source_select" type="select" label="Lastz settings to use" help="For most mapping needs use Commonly used settings. If you want full control use Full List"> - <option value="pre_set">Commonly used</option> - <option value="full">Full Parameter List</option> - </param> - <when value="pre_set"> - <param name="pre_set_options" type="select" label="Select mapping mode"> - <option value="yasra98">Roche-454 98% identity</option> - <option value="yasra95">Roche-454 95% identity</option> - <option value="yasra90">Roche-454 90% identity</option> - <option value="yasra85">Roche-454 85% identity</option> - <option value="yasra75">Roche-454 75% identity</option> - <option value="yasra95short">Illumina 95% identity</option> - <option value="yasra85short">Illumina 85% identity</option> - </param> - </when> - <when value="full"> - <param name="strand" type="select" label="Which strand to search?"> - <option value="both">Both</option> - <option value="plus">Search forward strand only (the one in the reference)</option> - <option value="minus">Search the reverse complement strand only (opposite of the reference)</option> - </param> - <param name="seed" type="select" label="Select seeding settings" help="allows you set word size and number of mismatches"> - <option value="12of19">Seed hits require a 19 bp word with matches in 12 specific positions</option> - <option value="14of22">Seed hits require a 22 bp word with matches in 14 specific positions</option> - </param> - <param name="transition" type="select" label="Select transition settings" help="affects the number of allowed transition substitutions"> - <option value="transition">Allow one transition in each seed hit</option> - <option value="transition=2">Allow two transitions in a seed hit </option> - <option value="notransition">Don't allow any transitions in seed hits</option> - </param> - <param name="gfextend" type="select" label="Perform gap-free extension of seed hits to HSPs (high scoring segment pairs)?"> - <option value="nogfextend">No</option> - <option value="gfextend">Yes</option> - </param> - <param name="chain" type="select" label="Perform chaining of HSPs?"> - <option value="nochain">No</option> - <option value="chain">Yes</option> - </param> - <param name="O" type="integer" value="400" label="Gap opening penalty"/> - <param name="E" type="integer" value="30" label="Gap extension penalty"/> - <param name="X" type="integer" value="910" label="X-drop threshold"/> - <param name="Y" type="integer" value="9370" label="Y-drop threshold"/> - <param name="K" type="integer" value="3000" label="Set the threshold for HSPs (ungapped extensions scoring lower are discarded)"/> - <param name="L" type="integer" value="3000" label="Set the threshold for gapped alignments (gapped extensions scoring lower are discarded)"/> - <param name="entropy" type="select" label="Involve entropy when filtering HSPs?"> - <option value="noentropy">No</option> - <option value="entropy">Yes</option> - </param> - </when> - </conditional> - <conditional name="seq_name"> - <param name="how_to_name" type="select" label="Do you want to modify the reference name?"> - <option value="no">No</option> - <option value="yes">Yes</option> - </param> - <when value="yes"> - <param name="ref_name" type="text" value="Type sequence name here" label="Enter name for the Reference sequence"/> - </when> - <when value="no" /> - </conditional> - <param name="min_ident" type="integer" value="0" label="Do not report matches below this identity (%)"/> - <param name="max_ident" type="integer" value="100" label="Do not report matches above this identity (%)"/> - <param name="min_cvrg" type="integer" value="0" label="Do not report matches that cover less than this percentage of each read"/> - <param name="unmask" type="select" label="Convert lowercase bases to uppercase"> - <option value="yes">Yes</option> - <option value="no">No</option> - </param> - </inputs> - <outputs> - <data format="tabular" name="output1" label="${tool.name} on ${on_string}: mapped reads"> - <change_format> - <when input="out_format" value="sam" format="sam" /> - </change_format> - </data> - </outputs> - <tests> - <test> - <!-- - Lastz command: - lastz phiX.2bit/phiX174[nickname=Ref] test-data/b1.fasta +nogfextend +nochain +gapped +strand=both +seed=12of19 +transition O=400 E=30 X=910 Y=9370 K=3000 L=3000 +noentropy +ambiguousn +nolaj +identity=0..100 +coverage=0 +format=sam- > lastz_wrapper_out2.sam - You need to point to phiX.2bit somewhere on your system. b1.fasta is located in galaxy's test-data. You will have to replace all the pluses before the - commands with 2 dashes, as double-dash can't appear in an XML comment. - --> - <param name="input2" value="b1.fasta" ftype="fasta" /> - <param name="ref_source" value="cached" /> - <param name="input1_2bit" value="phiX174" /> - <param name="out_format" value="sam" /> - <param name="source_select" value="full" /> - <param name="strand" value="both" /> - <param name="seed" value="12of19" /> - <param name="transition" value="transition" /> - <param name="gfextend" value="nogfextend" /> - <param name="chain" value="nochain" /> - <param name="O" value="400" /> - <param name="E" value="30" /> - <param name="X" value="910" /> - <param name="Y" value="9370" /> - <param name="K" value="3000" /> - <param name="L" value="3000" /> - <param name="entropy" value="noentropy" /> - <!-- - how_to_name is not the default. It is changed to modify - input1_2bit by adding the ref_name as a nickname - --> - <param name="how_to_name" value="yes" /> - <param name="ref_name" value="Ref" /> - <param name="min_ident" value="0" /> - <param name="max_ident" value="100" /> - <param name="min_cvrg" value="0" /> - <param name="unmask" value="yes" /> - <output name="output1" file="lastz_wrapper_out2.sam" /> - </test> - <test> - <!-- - Lastz command: - lastz test-data/phiX.fasta test-data/b1.fasta[fullnames] +yasra95short +ambiguousn +nolaj +identity=0..100 +coverage=0 +format=diffs > lastz_wrapper_out3.tabular - phiX.fasta and b1.fasta are located in galaxy's test-data. You will have to replace all the pluses before the commands with 2 dashes, - as double-dash can't appear in an XML comment. - --> - <param name="input2" value="b1.fasta" ftype="fasta" /> - <param name="ref_source" value="history" /> - <param name="input1" value="phiX.fasta" ftype="fasta" /> - <param name="out_format" value="diffs" /> - <param name="source_select" value="pre_set" /> - <param name="pre_set_options" value="yasra95short" /> - <param name="how_to_name" value="no" /> - <param name="min_ident" value="0" /> - <param name="max_ident" value="100" /> - <param name="min_cvrg" value="0" /> - <param name="unmask" value="yes" /> - <output name="output1" file="lastz_wrapper_out3.tabular" /> - </test> - <test> - <!-- - Lastz command: first you will need to split the file phiX_split.fasta into two files, - phiX1.fasta and phiX2.fasta, each with 1 sequence (phiX1 and phiX2, respectively). Then: - lastz phiX1.fasta test-data/b1.fasta *yasra95short *ambiguousn *nolaj *identity=0..100 *coverage=0 *format=general-:score,name1,strand1,size1,start1,zstart1,end1,length1,text1,name2,strand2,size2,start2,zstart2,end2,start2+,zstart2+,end2+,length2,text2,diff,cigar,identity,coverage,gaprate,diagonal,shingle > lastz_wrapper_out4.tabular - lastz phiX2.fasta test-data/b1.fasta *yasra95short *ambiguousn *nolaj *identity=0..100 *coverage=0 *format=general-:score,name1,strand1,size1,start1,zstart1,end1,length1,text1,name2,strand2,size2,start2,zstart2,end2,start2+,zstart2+,end2+,length2,text2,diff,cigar,identity,coverage,gaprate,diagonal,shingle >> lastz_wrapper_out4.tabular - You need to point to phiX1.fasta and phiX2.fasta somewhere on your system. - phiX_split.fasta and b1.fasta are located in galaxy's test-data - You will have to replace all the asterisks before the commands with 2 dashes, - as double-dash can't appear in an XML comment - - NOTE: since the input file include more than 1 sequence, the output must be sorted in - order for functional test to pass. This is done using the sort="True" attribute on the output. - --> - <param name="input2" value="b1.fasta" ftype="fasta" /> - <param name="ref_source" value="history" /> - <param name="input1" value="phiX_split.fasta" ftype="fasta" /> - <param name="out_format" value="tabular" /> - <param name="source_select" value="pre_set" /> - <param name="pre_set_options" value="yasra95short" /> - <param name="how_to_name" value="no" /> - <param name="min_ident" value="0" /> - <param name="max_ident" value="100" /> - <param name="min_cvrg" value="0" /> - <param name="unmask" value="yes" /> - <output name="output1" file="lastz_wrapper_out4.tabular" sort="True" /> - </test> - </tests> - <help> - -**What it does** - -**LASTZ** is a high performance pairwise sequence aligner derived from BLASTZ. It is written by Bob Harris in Webb Miller's laboratory at Penn State University. Special scoring sets were derived to improve runtime performance and quality. This Galaxy version of LASTZ is geared towards aligning short (Illumina/Solexa, AB/SOLiD) and medium (Roche/454) reads against a reference sequence. There is excellent, extensive documentation on LASTZ available here_. - - .. _here: http://www.bx.psu.edu/miller_lab/dist/README.lastz-1.02.00/README.lastz-1.02.00.html - ------- - -**Input formats** - -LASTZ accepts reference and reads in FASTA format. However, because Galaxy supports implicit format conversion the tool will recognize fastq and other method specific formats. - ------- - -**Outputs** - -LASTZ generates one output. Depending on the choice you make in the *Select output format* drop-down, LASTZ will produce a SAM file showing sequence alignments, a list of differences between the reads and reference (Polymorphisms), or a general table with one line per alignment block (Tabular). Examples of these outputs are shown below. - -**SAM output** - -SAM has 12 columns:: - - 1 2 3 4 5 6 7 8 9 10 11 12 - ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- - HWI-EAS91_1_30788AAXX:1:2:1670:915 99 chr9 58119878 60 36M = 58120234 392 GACCCCTACCCCACCGTGCTCTGGATCTCAGTGTTT IIIIIIIIIIIIIIIIEIIIIIII7IIIIIIIIIII XT:A:U NM:i:0 SM:i:37 AM:i:37 X0:i:1 X1:i:0 XM:i:0 XO:i:0 XG:i:0 MD:Z:36 - HWI-EAS91_1_30788AAXX:1:2:1670:915 147 chr9 58120234 60 36M = 58119878 -392 ATGAGTCGAATTCTATTTTCCAAACTGTTAACAAAA IFIIDI;IIICIIIIIIIIIIIIIIIIIIIIIIIII XT:A:U NM:i:0 SM:i:37 AM:i:37 X0:i:1 X1:i:0 XM:i:0 XO:i:0 XG:i:0 MD:Z:36 - - -where:: - - Column Description - --------- --------------------------------------------------------------------- - 1. QNAME Query (pair) NAME - 2. FLAG bitwise FLAG - 3. RNAME Reference sequence NAME - 4. POS 1-based leftmost POSition/coordinate of clipped sequence - 5. MAPQ MAPping Quality (Phred-scaled) - 6. CIGAR extended CIGAR string - 7. MRNM Mate Reference sequence NaMe ('=' if same as RNAME) - 8. MPOS 1-based Mate POSition - 9. ISIZE Inferred insert SIZE - 10. SEQ query SEQuence on the same strand as the reference - 11. QUAL query QUALity (ASCII-33 gives the Phred base quality) - 12. OPT variable OPTional fields in the format TAG:VTYPE:VALUE, tab-separated - -The flags are as follows:: - - Flag Description - ------ ------------------------------------- - 0x0001 the read is paired in sequencing - 0x0002 the read is mapped in a proper pair - 0x0004 the query sequence itself is unmapped - 0x0008 the mate is unmapped - 0x0010 strand of the query (1 for reverse) - 0x0020 strand of the mate - 0x0040 the read is the first read in a pair - 0x0080 the read is the second read in a pair - 0x0100 the alignment is not primary - -**Polymorphism (SNP or differences) output** - -Polymorphism output contains 14 columns:: - - 1 2 3 4 5 6 7 8 9 10 11 12 13 14 - -------------------------------------------------------------------------------------------------------------------------------------------------------------- - chrM 2490 2491 + 5386 HWI-EAS91_1_306UPAAXX:6:1:486:822 10 11 - 36 C A ACCTGTTTTACAGACACCTAAAGCTACATCGTCAAC ACCTGTTTTAAAGACACCTAAAGCTACATCGTCAAC - chrM 2173 2174 + 5386 HWI-EAS91_1_306UPAAXX:6:1:259:1389 26 27 + 36 G T GCGTACTTATTCGCCACCATGATTATGACCAGTGTT GCGTACTTATTCGCCACCATGATTATTACCAGTGTT - -where:: - - 1. (chrM) - Reference sequence id - 2. (2490) - Start position of the difference in the reference - 3. (2491) - End position of the difference in the reference - 4. (+) - Strand of the reference (always plus) - 5. (5386) - Length of the reference sequence - 6. (HWI...) - read id - 7. (10) - Start position of the difference in the read - 8. (11) - End position of the difference in the read - 9. (+) - Strand of the read - 10. (36) - Length of the read - 11. (C) - Nucleotide in the reference - 12. (A) - Nucleotide in the read - 13. (ACC...) - Reference side os the alignment - 14. (ACC...) - Read side of the alignment - -**Tabular output** - -Tabular output is a tab-separated format with 30 columns:: - - 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 - ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- - 14 PHIX174 + 5386 4648 4647 4661 14 ATTTTCGTGATATT EYKX4VC01BV8HS + 204 154 153 167 154 153 167 14 ATTTTCGTGATATT .............. 14M 14/14 100.0% 14/204 6.9% 0/14 0.0% 4494 NA - 16 PHIX174 + 5386 3363 3362 3378 16 GACGCCGGATTTGAGA EYKX4VC01AWJ88 - 259 36 35 51 209 208 224 16 GACGCCGGATTTGAGA ................ 16M 16/16 100.0% 16/259 6.2% 0/16 0.0% 3327 NA - -The following columns are present:: - - Field Meaning - ---------------- ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- - 1. score Score of the alignment block. The scale and meaning of this number will vary, depending on the final stage performed and other command-line options. - 2. name1 Name of the target sequence. - 3. strand1 Target sequence strand, either "+" or "−". - 4. size1 Size of the entire target sequence. - 5. start1 Starting position of the alignment block in the target, origin-one. - 6. zstart1 Starting position of the alignment block in the target, origin-zero. - 7. end1 Ending position of the alignment block in the target, expressed either as origin-one closed or origin-zero half-open (the ending value is the same in both systems). - 8. length1 Length of the alignment block in the target (excluding gaps). - 9. text1 Aligned characters in the target, including gap characters. - 10. name2 Name of the query sequence. - 11. strand2 Query sequence strand, either "+" or "−". - 12. size2 Size of the entire query sequence. - 13. start2 Starting position of the alignment block in the query, origin-one. - 14. zstart2 Starting position of the alignment block in the query, origin-zero. - 15. end2 Ending position of the alignment block in the query, expressed either as origin-one closed or origin-zero half-open (the ending value is the same in both systems). - 16. start2+ Starting position of the alignment block in the query, counting along the query sequence's positive strand (regardless of which query strand was aligned), origin-one. Note that if strand2 is "−", then this is the other end of the block from start2. - 17. zstart2+ Starting position of the alignment block in the query, counting along the query sequence's positive strand (regardless of which query strand was aligned), origin-zero. Note that if strand2 is "−", then this is the other end of the block from zstart2. - 18. end2+ Ending position of the alignment block in the query, counting along the query sequence's positive strand (regardless of which query strand was aligned), expressed either as origin-one closed or origin-zero half-open (the ending value is the same in both systems). Note that if strand2 is "−", then this is the other end of the block from end2. - 19. length2 Length of the alignment block in the query (excluding gaps). - 20. text2 Aligned characters in the query, including gap characters. - 21. diff Differences between what would be written for text1 and text2. Matches are written as . (period), transitions as : (colon), transversions as X, and gaps as - (hyphen). - 22. cigar A CIGAR-like representation of the alignment's path through the Dynamic Programming matrix. This is the short representation, without spaces, described in the Ensembl CIGAR specification. - 23./24. identity Fraction of aligned bases in the block that are matches (see Identity). This is written as two fields. The first field is a fraction, written as <n>/<d>. The second field contains the same value, computed as a percentage. - 25./26. coverage Fraction of the entire input sequence (target or query, whichever is shorter) that is covered by the alignment block (see Coverage). This is written as two fields. The first field is a fraction, written as <n>/<d>. The second field contains the same value, computed as a percentage. - 27./28. gaprate Rate of gaps (also called indels) in the alignment block. This is written as two fields. The first field is a fraction, written as <n>/<d>, with the numerator being the number of alignment columns containing gaps and the denominator being the number without gaps. The second field contains the same value, computed as a percentage. - 29. diagonal The diagonal of the start of the alignment block in the dynamic programming matrix, expressed as an identifying number start1-start2. - 30. shingle A measurement of the shingle overlap between the target and the query. This is intended for the case where both the target and query are relatively short, and their ends are expected to overlap. - -------- - -**LASTZ Settings** - -There are two setting modes: (1) **Commonly used settings** and (2) **Full Parameter List**. - -**Commonly used settings** - -There are seven modes:: - - Illumina-Solexa/AB-SOLiD 95% identity - Illumina-Solexa/AB-SOLiD 85% identity - Roche-454 98% identity - Roche-454 95% identity - Roche-454 90% identity - Roche-454 85% identity - Roche-454 75% identity - -When deciding which one to use, consider the following: a 36 bp read with two differences will be 34/36 = 94% identical to the reference. - -**Full Parameter List** - -This mode gives you fuller control over lastz. The description of these and other parameters is found at the end of this page. Note that not all parameters are included in this interface. If you would like to make additional options available through Galaxy, e-mail us at galaxy-bugs@bx.psu.edu. - ------- - -**Do you want to modify the reference name?** - -This option allows you to set the name of the reference sequence manually. This is helpful when, for example, you would like to make the reference name compatible with the UCSC naming conventions to be able to display your lastz results as a custom track at the UCSC Genome Browser. - ------- - -**LASTZ parameter list** - -This is an exhaustive list of LASTZ options. Once again, please note that not all options are included in this interface. If you would like to make additional options available through Galaxy, e-mail us at galaxy-bugs@bx.psu.edu:: - - target[[s..e]][-] spec/file containing target sequence (fasta or nib) - [s..e] defines a subrange of the file - - indicates reverse-complement - (use --help=files for more details) - query[[s..e]][-] spec/file containing query sequences (fasta or nib) - if absent, queries come from stdin (unless they - aren't needed, as for --self or --tableonly) - (use --help=files for more details) - --self the target sequence is also the query - --quantum the query sequence contains quantum DNA - --seed=match<length> use a word with no gaps instead of a seed pattern - --seed=half<length> use space-free half-weight word instead of seed pattern - --match=<reward>[,<penalty>] set the score values for a match (+<reward>) - and mismatch (-<penalty>) - --[no]trans[ition][=2] allow one or two transitions in a seed hit - (by default a transition is allowed) - --word=<bits> set max bits for word hash; use this to trade time for - memory, eliminating thrashing for heavy seeds - (default is 28 bits) - --[no]filter=[<T>:]<M> filter half-weight seed hits, requiring at least M - matches and allowing no more than T transversions - (default is no filtering) - --notwins require just one seed hit - --twins=[<min>:]<maxgap> require two nearby seed hits on the same diagonal - (default is twins aren't required) - --notwins allow single, isolated seeds - --[no]recoverseeds avoid losing seeds in hash collisions. Cannot be used with --twins - --seedqueue=<entries> set number of entries in seed hit queue - (default is 262144) - --anchors=<file> read anchors from a file, instead of discovering anchors - via seeding - --recoverhits recover hash-collision seed hits - (default is not to recover seed hits) - --step=<length> set step length (default is 1) - --maxwordcount=<limit> words occurring more often than <limit> in the target - are not eligible for seeds - --strand=both search both strands - --strand=plus search + strand only (matching strand of query spec) - --strand=minus search - strand only (opposite strand of query spec) - (by default both strands are searched) - --ambiguousn treat N as an ambiguous nucleotide - (by default N is treated as a sequence splicing character) - --[no]gfextend perform gap-free extension of seed hits to HSPs - (by default no extension is performed) - --[no]chain perform chaining - --chain=<diag,anti> perform chaining with given penalties for diagonal and - anti-diagonal - (by default no chaining is performed) - --[no]gapped perform gapped alignment (instead of gap-free) - (by default gapped alignment is performed) - --score[s]=<file> read substitution scores from a file - (default is HOXD70) - --unitscore[s] scores are +1/-1 for match/mismatch - --gap=<[open,]extend> set gap open and extend penalties (default is 400,30) - --xdrop=<score> set x-drop threshold (default is 10*sub[A][A]) - --ydrop=<score> set y-drop threshold (default is open+300extend) - --infer[=<control>] infer scores from the sequences, then use them - --inferonly[=<control>] infer scores, but don't use them (requires --infscores) - all inference options are read from the control file - --infscores[=<file>] write inferred scores to a file - --hspthresh=<score> set threshold for high scoring pairs (default is 3000) - ungapped extensions scoring lower are discarded - <score> can also be a percentage or base count - --entropy adjust for entropy when qualifying HSPs in the x-drop extension - method - --noentropy don't adjust for entropy when qualifying HSPs - --exact=<length> set threshold for exact matches - if specified, exact matches are found rather than high - scoring pairs (replaces --hspthresh) - --inner=<score> set threshold for HSPs during interpolation - (default is no interpolation) - --gappedthresh=<score> set threshold for gapped alignments - gapped extensions scoring lower are discarded - <score> can also be a percentage or base count - (default is to use same value as --hspthresh) - --ball=<score> set minimum score required of words 'in' a quantum ball - --[no]entropy involve entropy in filtering high scoring pairs - (default is "entropy") - --[no]mirror report/use mirror image of all gap-free alignments - (default is "mirror" for self-alignments only) - --traceback=<bytes> space for trace-back information - (default is 80.0M) - --masking=<count> mask any position in target hit this many times - zero indicates no masking - (default is no masking) - --targetcapsule=<capsule_file> the target seed word position table and seed - (as well as the target sequence)are read from specified file - --segments=<segment_file> read segments from a file, instead of discovering - them via seeding. Replaces other seeding or gap-free extension - options - --[no]census[=<file>] count/report how many times each target base aligns - (default is to not report census) - --identity=<min>[..<max>] filter alignments by percent identity - 0<=min<=max<=100; blocks (or HSPs) outside min..max - are discarded - (default is no identity filtering) - --coverage=<min>[..<max>] filter alignments by percentage pf query covered - 0<=min<=max<=100; blocks (or HSPs) outside min..max - are discarded - (default is no query coverage filtering) - --notrivial do not output trivial self-alignment block if the target and query - sequences are identical. Using --self enables this option automatically - --output=<output_file> write the alignments to the specified file name instead of stdout - --code=<file> give quantum code for query sequence (only for display) - --format=<type> specify output format; one of lav, axt, maf, maf+, maf-, text, - lav+text, cigar, text, rdplot, general, or general:<fields> - (by default output is LAV) - --rdotplot=<file> create an additional output file suitable for plotting the alignments - with the R statistical package. - --markend Just before normal completion, write "# lastz end-of-file" to output file - --census[=<output_file>] count and report how many times each target base aligns, up - to 255. Ns are included in the count - --census16[=<output_file>] count and report how many times each target base aligns, up - up 65 thousand - --census32[=<output_file>] count and report how many times each target bas aligns, up - to 4 billion - --writecapsule=<capsule_file> just write out a target capsule file and quit; don't - search for seeds or perform subsequent stages - --verbosity=<level> set info level (0 is minimum, 10 is everything) - (default is 0) - --[no]runtime report runtime in the output file - (default is to not report runtime) - --tableonly[=count] just produce the target position table, don't - search for seeds - --[no]stats[=<file>] show search statistics (or don't) - (not available in this build) - --version report the program version and quit - --help list all options - --help=files list information about file specifiers - --help=short[cuts] list blastz-compatible shortcuts - --help=yasra list yasra-specific shortcuts - - </help> -</tool>
--- a/test-data/b1.fasta Tue Oct 13 12:23:37 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1000 +0,0 @@ ->HWI-EAS91_1_306UPAAXX:6:1:1503:1160 -GGTGGTCTATAGTGTTATTAATATCAAGTTGGGGGG ->HWI-EAS91_1_306UPAAXX:6:1:1564:1179 -GCGAGCAGTAGACTCCTTCTGTTGATAAGCAAGCAT ->HWI-EAS91_1_306UPAAXX:6:1:1704:1082 -GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC ->HWI-EAS91_1_306UPAAXX:6:1:1588:1797 -GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT ->HWI-EAS91_1_306UPAAXX:6:1:1304:1526 -GTAGTTGAAATGGTAATAAGACGACCAATCTGACCT ->HWI-EAS91_1_306UPAAXX:6:1:1490:1582 -GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC ->HWI-EAS91_1_306UPAAXX:6:1:1356:1339 -GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC ->HWI-EAS91_1_306UPAAXX:6:1:1311:853 -GGTTGGTTTATCGTTTTTGACACTCTCACGTTGTCT ->HWI-EAS91_1_306UPAAXX:6:1:1257:1552 -GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC ->HWI-EAS91_1_306UPAAXX:6:1:1486:1402 -GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC ->HWI-EAS91_1_306UPAAXX:6:1:1028:1081 -GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT ->HWI-EAS91_1_306UPAAXX:6:1:1167:752 -GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC ->HWI-EAS91_1_306UPAAXX:6:1:1507:1113 -GTCAACGTTATATTTTGATAGTTTGACGGTTAATTC ->HWI-EAS91_1_306UPAAXX:6:1:1654:1311 -GGATGAAAATGCTCACAATGACAAATCTGTCCACGG ->HWI-EAS91_1_306UPAAXX:6:1:1386:1060 -GTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTT ->HWI-EAS91_1_306UPAAXX:6:1:1070:1356 -GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG ->HWI-EAS91_1_306UPAAXX:6:1:787:1032 -GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA ->HWI-EAS91_1_306UPAAXX:6:1:834:1017 -GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT ->HWI-EAS91_1_306UPAAXX:6:1:1703:1155 -GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT ->HWI-EAS91_1_306UPAAXX:6:1:1406:593 -GTTGAGTTCGATAATGGTGATATGTATGTTTACGGC ->HWI-EAS91_1_306UPAAXX:6:1:1411:886 -GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT ->HWI-EAS91_1_306UPAAXX:6:1:923:972 -GCATGACAAGTAAAGGACGGTTGTCAGCGTCATAAG ->HWI-EAS91_1_306UPAAXX:6:1:1279:1004 -GCCATAGCACCAGAAACAAAACTAGGGGCGGCCTCT ->HWI-EAS91_1_306UPAAXX:6:1:1070:840 -GGTTGTCAGCGTCATAAGAGGTTTTACCTCCAAATG ->HWI-EAS91_1_306UPAAXX:6:1:1595:1040 -GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT ->HWI-EAS91_1_306UPAAXX:6:1:1002:559 -GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG ->HWI-EAS91_1_306UPAAXX:6:1:999:974 -GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC ->HWI-EAS91_1_306UPAAXX:6:1:896:982 -GTGGCTGGAGACAAATAATCTCTTTAATAACCTTAT ->HWI-EAS91_1_306UPAAXX:6:1:1366:741 -GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC ->HWI-EAS91_1_306UPAAXX:6:1:749:1469 -GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT ->HWI-EAS91_1_306UPAAXX:6:1:1010:592 -GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT ->HWI-EAS91_1_306UPAAXX:6:1:1393:650 -GTGACTCATATCTAAACCAGTCCTTGACGAACGTGC ->HWI-EAS91_1_306UPAAXX:6:1:1238:1731 -GAGAAATAAAAGTCTGAAACATGATTAAACTCCTAA ->HWI-EAS91_1_306UPAAXX:6:1:1629:908 -GATGCGGTTATCCATCTGCTTATGGAAGCCAAGCAT ->HWI-EAS91_1_306UPAAXX:6:1:1560:849 -GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG ->HWI-EAS91_1_306UPAAXX:6:1:1029:783 -GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT ->HWI-EAS91_1_306UPAAXX:6:1:1152:1324 -GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG ->HWI-EAS91_1_306UPAAXX:6:1:1614:2042 -GAAATGCCACAAGCCTCAATAGCAGGTTTAAGAGCC ->HWI-EAS91_1_306UPAAXX:6:1:1398:439 -GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG ->HWI-EAS91_1_306UPAAXX:6:1:955:616 -GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC ->HWI-EAS91_1_306UPAAXX:6:1:1672:753 -GAATGCCAGCAATCTCTTTTTGAGTCTCATTTTGCT ->HWI-EAS91_1_306UPAAXX:6:1:1195:1293 -GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC ->HWI-EAS91_1_306UPAAXX:6:1:1074:755 -GCAAGAGTAAACATAGTGCCATGCTCAGGAACAAAG ->HWI-EAS91_1_306UPAAXX:6:1:984:499 -GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT ->HWI-EAS91_1_306UPAAXX:6:1:1452:1833 -GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT ->HWI-EAS91_1_306UPAAXX:6:1:863:710 -GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT ->HWI-EAS91_1_306UPAAXX:6:1:885:649 -GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC ->HWI-EAS91_1_306UPAAXX:6:1:917:1214 -GACAGATGTATCCATCTGAATGCAATGAAGAAAACC ->HWI-EAS91_1_306UPAAXX:6:1:892:1254 -GCTCAGGAAATGCAGCAGCAAGATAATCACGAGTAT ->HWI-EAS91_1_306UPAAXX:6:1:1555:1005 -GCATTTGGCGCATAATCTCGGAAACCTGCTGTTGCT ->HWI-EAS91_1_306UPAAXX:6:1:1637:1413 -GATGCTGTTCAACCACTAATAGGTAAGAAATCATGT ->HWI-EAS91_1_306UPAAXX:6:1:1102:1567 -GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA ->HWI-EAS91_1_306UPAAXX:6:1:799:1337 -GTATATGCACAAAATGAGATGCTTGCTTATCAACAG ->HWI-EAS91_1_306UPAAXX:6:1:1353:1843 -GCAGACCCATAATGTCAATAGATGTGGTAGAAGTCG ->HWI-EAS91_1_306UPAAXX:6:1:1196:789 -GCGGCATACGCTCGGCGCCAGTTTGAATATTAGACA ->HWI-EAS91_1_306UPAAXX:6:1:1056:1676 -GTAAAATACTGACCAGCCGTTTGAGCTTGAGTAAGC ->HWI-EAS91_1_306UPAAXX:6:1:1349:1836 -GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC ->HWI-EAS91_1_306UPAAXX:6:1:1027:788 -GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT ->HWI-EAS91_1_306UPAAXX:6:1:990:1283 -GCTTAGGGATTTTATTGGTATCAGGGTTAATCGTGC ->HWI-EAS91_1_306UPAAXX:6:1:904:939 -GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT ->HWI-EAS91_1_306UPAAXX:6:1:1732:793 -GTCAACATACATATCACCATTATCGAACTCAACGCC ->HWI-EAS91_1_306UPAAXX:6:1:1355:2003 -GTTAGACCAAACCATGAAACCAACATAAACATTATT ->HWI-EAS91_1_306UPAAXX:6:1:1337:977 -GCACCAGAAACAAAACTAGGGGCGGCCTCATCAGGG ->HWI-EAS91_1_306UPAAXX:6:1:1605:1175 -GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT ->HWI-EAS91_1_306UPAAXX:6:1:1763:1192 -GACAGGCCGTTTGAATGTTGACGGGATGAACATAAT ->HWI-EAS91_1_306UPAAXX:6:1:722:483 -GTTATTATACCGTCAAGGACTGTGTGACTATTGACT ->HWI-EAS91_1_306UPAAXX:6:1:1760:1136 -GCAAAGCATTGGGATTATCATAAAACGCCTCTAATC ->HWI-EAS91_1_306UPAAXX:6:1:1088:798 -GGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTC ->HWI-EAS91_1_306UPAAXX:6:1:633:1076 -GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG ->HWI-EAS91_1_306UPAAXX:6:1:673:754 -GTCATGGAAGCGATAAAACTCTGCAGGTTGGATATT ->HWI-EAS91_1_306UPAAXX:6:1:1759:2019 -GTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTAC ->HWI-EAS91_1_306UPAAXX:6:1:1064:1797 -GCGGTTATCCATCTGCTTATGGAAGCCAAGCATTGG ->HWI-EAS91_1_306UPAAXX:6:1:1112:1669 -GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT ->HWI-EAS91_1_306UPAAXX:6:1:510:1447 -GCATTAAGCTCAGGAAATGCAGCAGCAAGATAATCA ->HWI-EAS91_1_306UPAAXX:6:1:877:1573 -GTGCTATTGCTGGCGGTATTTCTTCTTCTTTTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:870:542 -GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC ->HWI-EAS91_1_306UPAAXX:6:1:966:384 -GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA ->HWI-EAS91_1_306UPAAXX:6:1:1186:1903 -GCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAG ->HWI-EAS91_1_306UPAAXX:6:1:1632:1742 -GCATCACCCATGCCTACAGTATTGTTATCGGTAGCC ->HWI-EAS91_1_306UPAAXX:6:1:1521:559 -GAGAGCGCCAACGGCGTCCATCTCGAAGGAGTCGCC ->HWI-EAS91_1_306UPAAXX:6:1:683:454 -GCTTATTATGTTCATCCCGTCAACATTCAAACGTCC ->HWI-EAS91_1_306UPAAXX:6:1:112:1280 -GTTGGCGCTCTCCGTCTTTCTCCATTTCGTCGTGTC ->HWI-EAS91_1_306UPAAXX:6:1:891:381 -GACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAG ->HWI-EAS91_1_306UPAAXX:6:1:1348:958 -GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT ->HWI-EAS91_1_306UPAAXX:6:1:1785:1915 -GCCCCGAAGGGGACNANAAATGGTTTTTAGAGAACG ->HWI-EAS91_1_306UPAAXX:6:1:1418:42 -GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT ->HWI-EAS91_1_306UPAAXX:6:1:1421:743 -GGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAAT ->HWI-EAS91_1_306UPAAXX:6:1:1079:790 -GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT ->HWI-EAS91_1_306UPAAXX:6:1:663:740 -GGTATTAAGGATGAGTGTTCAAGATTGCTGGATGCC ->HWI-EAS91_1_306UPAAXX:6:1:1245:413 -GTTTGAATGTTGACGGGATGAACATAATAAGCAATG ->HWI-EAS91_1_306UPAAXX:6:1:1378:1035 -GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGG ->HWI-EAS91_1_306UPAAXX:6:1:903:1746 -GTACGGGGAAGGACGTCAATAGTCACACAGTCCTTG ->HWI-EAS91_1_306UPAAXX:6:1:1713:1134 -GGCGTACGGGGAAGGACGTCAATAGTCACACAGTCC ->HWI-EAS91_1_306UPAAXX:6:1:1246:1887 -GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG ->HWI-EAS91_1_306UPAAXX:6:1:872:1731 -GGGCGGCCTCATCAGGGTTAGGAACATTAGAGCCTT ->HWI-EAS91_1_306UPAAXX:6:1:1714:1582 -GCTTTCCTGCTCCTGTTGAGTTTATTGCTTCCGTCT ->HWI-EAS91_1_306UPAAXX:6:1:1785:763 -GNCGAGAAATAAAANNNTGAAACATGATTAAANTCC ->HWI-EAS91_1_306UPAAXX:6:1:1684:542 -GAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGT ->HWI-EAS91_1_306UPAAXX:6:1:1581:1665 -GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT ->HWI-EAS91_1_306UPAAXX:6:1:901:1581 -GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT ->HWI-EAS91_1_306UPAAXX:6:1:1128:239 -GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC ->HWI-EAS91_1_306UPAAXX:6:1:969:441 -GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG ->HWI-EAS91_1_306UPAAXX:6:1:630:1087 -GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC ->HWI-EAS91_1_306UPAAXX:6:1:606:1852 -GGAGACAAATAATCTCTTTAATAACCTGATTCAGCG ->HWI-EAS91_1_306UPAAXX:6:1:489:1315 -GAAAGCTCAGTCTCAGGAGGAAGCGGAGCAGTCCAC ->HWI-EAS91_1_306UPAAXX:6:1:465:1983 -GAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAA ->HWI-EAS91_1_306UPAAXX:6:1:559:1028 -GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC ->HWI-EAS91_1_306UPAAXX:6:1:1655:1413 -GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG ->HWI-EAS91_1_306UPAAXX:6:1:980:605 -GCCGTTTGAATGTTGACGGGATGAACATAATAAGCA ->HWI-EAS91_1_306UPAAXX:6:1:1629:1865 -GAAAAGCGGCATGGTCAATATAACCAGTAGTGTTAT ->HWI-EAS91_1_306UPAAXX:6:1:1180:1920 -GCACTCCGTGGACAGATTTGTCATTGTGAGCATTTT ->HWI-EAS91_1_306UPAAXX:6:1:1116:383 -GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG ->HWI-EAS91_1_306UPAAXX:6:1:906:2041 -GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA ->HWI-EAS91_1_306UPAAXX:6:1:1514:157 -GTCAATAGATGTGGTAGAAGTCGTCATTTGGCGTGG ->HWI-EAS91_1_306UPAAXX:6:1:1032:1857 -GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT ->HWI-EAS91_1_306UPAAXX:6:1:638:609 -GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAT ->HWI-EAS91_1_306UPAAXX:6:1:74:750 -GATAATCACGAGTATCCTTTCCTTTATCATCTTCAT ->HWI-EAS91_1_306UPAAXX:6:1:486:822 -GTTGACGATGTAGCTTTAGGTGTCTTTAAAACAGGT ->HWI-EAS91_1_306UPAAXX:6:1:899:473 -GAACAGCATCGGACTCAGATAGTAATCCACGCTCTT ->HWI-EAS91_1_306UPAAXX:6:1:1613:197 -GTGACATTCAGAAGGGTAATAAGAACGAACCATAAA ->HWI-EAS91_1_306UPAAXX:6:1:326:1747 -GTTGAGGCTTTCGTTTATTGTACGCTTTGCTTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:1487:526 -GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT ->HWI-EAS91_1_306UPAAXX:6:1:629:665 -GAAATGCAGCAGCAAGATAATCACGAGTATCCTTTC ->HWI-EAS91_1_306UPAAXX:6:1:766:744 -GGCCGTCAACATACATATCACCATTATCGAACTCAA ->HWI-EAS91_1_306UPAAXX:6:1:391:1771 -GTGGTTGATATTTTTCATGGTATTGATAAATCTTTT ->HWI-EAS91_1_306UPAAXX:6:1:591:1102 -GCTTTGCGTGACTATTTTCGTGATATTGTTCGTTTG ->HWI-EAS91_1_306UPAAXX:6:1:917:664 -GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG ->HWI-EAS91_1_306UPAAXX:6:1:217:737 -GTTCAGTTGTTGCATTGGAATATTCAGTTTAAATTT ->HWI-EAS91_1_306UPAAXX:6:1:1047:839 -GACCATTCAAAGGATAAACATCATAGGCAGTCGGGG ->HWI-EAS91_1_306UPAAXX:6:1:558:1040 -GCCACCAGCAAGAGCAGAAGCAATACCGCCAGCAAT ->HWI-EAS91_1_306UPAAXX:6:1:1207:524 -GCCAATACCATCAGCTTTACCGTCTTTCCAGAAATT ->HWI-EAS91_1_306UPAAXX:6:1:708:1634 -GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG ->HWI-EAS91_1_306UPAAXX:6:1:576:1851 -GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG ->HWI-EAS91_1_306UPAAXX:6:1:906:460 -GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC ->HWI-EAS91_1_306UPAAXX:6:1:693:1260 -GCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCG ->HWI-EAS91_1_306UPAAXX:6:1:1373:286 -GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG ->HWI-EAS91_1_306UPAAXX:6:1:762:41 -GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT ->HWI-EAS91_1_306UPAAXX:6:1:475:1091 -GTCACACAGTCCTTGACGGTATAATAACCACCATCT ->HWI-EAS91_1_306UPAAXX:6:1:791:627 -GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC ->HWI-EAS91_1_306UPAAXX:6:1:336:1791 -GAAGGAGTCGCCAGCGATAACCGGAGTAGTTGAAAT ->HWI-EAS91_1_306UPAAXX:6:1:1483:943 -GCACGTAATTTTTGACGCACGTTTTCTTCTGCGTCT ->HWI-EAS91_1_306UPAAXX:6:1:641:1071 -GATGGGCATACTGTAACCATAAGGCCACGTATTTTG ->HWI-EAS91_1_306UPAAXX:6:1:196:755 -GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT ->HWI-EAS91_1_306UPAAXX:6:1:463:1398 -GTCATAAGAGGTTTTACCTCCAAATGAAGAAATAAC ->HWI-EAS91_1_306UPAAXX:6:1:1559:460 -GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT ->HWI-EAS91_1_306UPAAXX:6:1:1625:1561 -GAGGAGTGGCATTAACACCATCCTTCATGAACTTAC ->HWI-EAS91_1_306UPAAXX:6:1:1729:1588 -GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT ->HWI-EAS91_1_306UPAAXX:6:1:945:393 -GGCCTCATCAGGGTTAGGAACATTAGAGCCTTGAAT ->HWI-EAS91_1_306UPAAXX:6:1:298:1391 -GTAAAGTTAGACCAAACCATGAAACCAACATAAACA ->HWI-EAS91_1_306UPAAXX:6:1:1270:1500 -GAATTACTACTGCTTGTTTACGAATTAAATATATGT ->HWI-EAS91_1_306UPAAXX:6:1:481:1546 -GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG ->HWI-EAS91_1_306UPAAXX:6:1:473:1729 -GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAT ->HWI-EAS91_1_306UPAAXX:6:1:801:1831 -GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT ->HWI-EAS91_1_306UPAAXX:6:1:536:639 -GCCGACCCTAAATTTTTTGCCTGTTTGGTTCTCTTT ->HWI-EAS91_1_306UPAAXX:6:1:259:938 -GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG ->HWI-EAS91_1_306UPAAXX:6:1:907:1513 -GGCATGGGTGATGCTGGTATTAAATCTGCCATTCAC ->HWI-EAS91_1_306UPAAXX:6:1:372:1409 -GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG ->HWI-EAS91_1_306UPAAXX:6:1:485:1626 -GATGGCAGCAACGGAAACCATAACGAGCATCATCTT ->HWI-EAS91_1_306UPAAXX:6:1:583:1679 -GCTCAAAGTCAAAATAATCAGCGTGACATTCAGAAG ->HWI-EAS91_1_306UPAAXX:6:1:690:1610 -GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT ->HWI-EAS91_1_306UPAAXX:6:1:257:918 -GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTTCT ->HWI-EAS91_1_306UPAAXX:6:1:818:33 -GTGTTAATGCCACTCCTCTCCCGACTGTTAACTCTG ->HWI-EAS91_1_306UPAAXX:6:1:541:1242 -GGGATTATCATAAAACGCCTCTAATCGGTCGTCAGC ->HWI-EAS91_1_306UPAAXX:6:1:1014:279 -GTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCC ->HWI-EAS91_1_306UPAAXX:6:1:672:1790 -GGCCGTTTGAATGTTGACGGGATGAACATAATAAGC ->HWI-EAS91_1_306UPAAXX:6:1:708:464 -GGAGACAAATAATCTCTTTAATAACCTGATTCAGCG ->HWI-EAS91_1_306UPAAXX:6:1:633:1486 -GGGAAAGGTCATGCGGCATACGCTCGGCGCCAGTTT ->HWI-EAS91_1_306UPAAXX:6:1:328:696 -GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT ->HWI-EAS91_1_306UPAAXX:6:1:259:1389 -GCGTACTTATTCGCCACCATGATTATTACCAGTGTT ->HWI-EAS91_1_306UPAAXX:6:1:1315:41 -GCTTTCCGTGATGTCACAGCCTGCTTTGATGTGTCG ->HWI-EAS91_1_306UPAAXX:6:1:1647:549 -GCTTAATCCAACTTACCAAGCTGGGTTACGACGCGC ->HWI-EAS91_1_306UPAAXX:6:1:300:886 -GTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTT ->HWI-EAS91_1_306UPAAXX:6:1:317:1411 -GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCTT ->HWI-EAS91_1_306UPAAXX:6:1:321:1819 -GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT ->HWI-EAS91_1_306UPAAXX:6:1:631:70 -GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC ->HWI-EAS91_1_306UPAAXX:6:1:624:1040 -GCTGGCGACTCCTTCGAGATGGACGCCGTTTGCGCT ->HWI-EAS91_1_306UPAAXX:6:1:662:1187 -GGGAGAGGAGTGGCATTAACACCATCCTTCATGACC ->HWI-EAS91_1_306UPAAXX:6:1:1440:1959 -GAATCAGCGGTATGGCTCCTCTCCTATTTTTGCTTC ->HWI-EAS91_1_306UPAAXX:6:1:458:1629 -GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT ->HWI-EAS91_1_306UPAAXX:6:1:216:790 -GGGATGAAAATGCTCACAATGACAAATCTGTCCACG ->HWI-EAS91_1_306UPAAXX:6:1:1407:1174 -TTACCTATTAGTGGTTGAACAGCATCGGACTCAGAT ->HWI-EAS91_1_306UPAAXX:6:1:999:1790 -GTCCTGCGTGTAGCGAACTGCGATGGGCATACTGTC ->HWI-EAS91_1_306UPAAXX:6:1:141:1994 -GGCTTTTTTATGGTTCGTTCTTATTACCCTTCTTAT ->HWI-EAS91_1_306UPAAXX:6:1:225:465 -GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC ->HWI-EAS91_1_306UPAAXX:6:1:649:1760 -GACCCATAATGTCAATAGATGTGGTAGAAGTCGTCT ->HWI-EAS91_1_306UPAAXX:6:1:300:986 -GTTGAACACGACCAGAAAACTGGCCTAACGACGTTT ->HWI-EAS91_1_306UPAAXX:6:1:478:605 -GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC ->HWI-EAS91_1_306UPAAXX:6:1:622:395 -GGTAGCTTTAAGCGGCTCACCTTTAGCATCAACAGG ->HWI-EAS91_1_306UPAAXX:6:1:1701:574 -GTAAAGCCTCTACGCGATTTCATAGTGGAGGCCTCC ->HWI-EAS91_1_306UPAAXX:6:1:646:59 -GGAAGTGTCCGCATAAAATGCACCGCATGGAAATGT ->HWI-EAS91_1_306UPAAXX:6:1:284:2031 -GACAGAATCGTTAGTTGATGGCGAAAGGTCGCAAAG ->HWI-EAS91_1_306UPAAXX:6:1:22:1009 -GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT ->HWI-EAS91_1_306UPAAXX:6:1:47:1826 -GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG ->HWI-EAS91_1_306UPAAXX:6:1:1025:1236 -TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT ->HWI-EAS91_1_306UPAAXX:6:1:773:591 -GAGCAGGAAAGCGAGGGTATCCCACAAAGTCCAGCG ->HWI-EAS91_1_306UPAAXX:6:1:1753:527 -GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC ->HWI-EAS91_1_306UPAAXX:6:1:426:1717 -GTAGCGCCAATATGAGAAGAGCCATACCGCTGATTC ->HWI-EAS91_1_306UPAAXX:6:1:959:818 -TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC ->HWI-EAS91_1_306UPAAXX:6:1:459:1344 -GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT ->HWI-EAS91_1_306UPAAXX:6:1:973:1367 -TTCGTGATGAGTTTGTATCTGTTACTGATAAGTTAT ->HWI-EAS91_1_306UPAAXX:6:1:201:871 -GATTAGAGGCGTTTTATGATAATCCCAATGCTTTTC ->HWI-EAS91_1_306UPAAXX:6:1:713:1672 -GGCGTACGGGGAAGGACGTCAATAGTCACACAGTCC ->HWI-EAS91_1_306UPAAXX:6:1:444:1435 -TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGTG ->HWI-EAS91_1_306UPAAXX:6:1:288:1136 -GCCTTCCATGATGAGACAGGCCGTTTTAATTTTTTC ->HWI-EAS91_1_306UPAAXX:6:1:1653:225 -GCAAGGCCACGACGCAATGGAGAAAGACGGAGAGCG ->HWI-EAS91_1_306UPAAXX:6:1:537:1764 -GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA ->HWI-EAS91_1_306UPAAXX:6:1:196:1854 -GTATCGAGGCTCTTAAACCTGCTATTTAGGCTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:312:1707 -GCGTCATAAGAGGTTTTACCTCCAAATGAAGAAATA ->HWI-EAS91_1_306UPAAXX:6:1:651:183 -GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT ->HWI-EAS91_1_306UPAAXX:6:1:295:694 -GTGATTACTTCATGCAGCGTTACCGTGATGTTATTT ->HWI-EAS91_1_306UPAAXX:6:1:330:1895 -GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG ->HWI-EAS91_1_306UPAAXX:6:1:590:331 -GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT ->HWI-EAS91_1_306UPAAXX:6:1:481:1687 -GCAGATTGCGATAAACGGTCACATTAAATTTAACCT ->HWI-EAS91_1_306UPAAXX:6:1:1112:1279 -TGTGCATATACCTGGTCTTTCGTATTCTGTCGTGAT ->HWI-EAS91_1_306UPAAXX:6:1:1099:1216 -TTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCG ->HWI-EAS91_1_306UPAAXX:6:1:221:1238 -GTATCCTTTCCTTTATCATCGGCAGACTTTTCACCT ->HWI-EAS91_1_306UPAAXX:6:1:1015:364 -GCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAG ->HWI-EAS91_1_306UPAAXX:6:1:735:1806 -TGTTATTAATATCAAGTTGGGGGAGCACATTGTAGC ->HWI-EAS91_1_306UPAAXX:6:1:320:411 -GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGTG ->HWI-EAS91_1_306UPAAXX:6:1:1273:1031 -TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA ->HWI-EAS91_1_306UPAAXX:6:1:1456:1088 -AATAATCAGCGTGACATTCAGAAGGGTAATAAGAAC ->HWI-EAS91_1_306UPAAXX:6:1:1365:307 -GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG ->HWI-EAS91_1_306UPAAXX:6:1:478:252 -GATGCGGTTATCCATCTGCTTATTGAAGCCAAGCAT ->HWI-EAS91_1_306UPAAXX:6:1:915:1232 -TATTAATAACACTATAGACCACCGCCCCGAAGGGGC ->HWI-EAS91_1_306UPAAXX:6:1:680:1357 -TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGC ->HWI-EAS91_1_306UPAAXX:6:1:238:1279 -GCCGAAGCCCCTGCAATTAAAATTGTTGACCACCTA ->HWI-EAS91_1_306UPAAXX:6:1:1583:35 -GCAAATTAGCATAAGCAGCTTGCAGACCCATAATGT ->HWI-EAS91_1_306UPAAXX:6:1:502:283 -GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT ->HWI-EAS91_1_306UPAAXX:6:1:418:1730 -GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT ->HWI-EAS91_1_306UPAAXX:6:1:596:647 -GCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTA ->HWI-EAS91_1_306UPAAXX:6:1:92:1591 -GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC ->HWI-EAS91_1_306UPAAXX:6:1:430:1938 -GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGTCC ->HWI-EAS91_1_306UPAAXX:6:1:212:527 -GGTATTGATAAAGCTGTTGCCGATACTTGTAACAAT ->HWI-EAS91_1_306UPAAXX:6:1:594:942 -GACGACATTAGAAATATCCTTTGCAGTAGCGCCAAT ->HWI-EAS91_1_306UPAAXX:6:1:169:1774 -GCCTTCCATGATGAGACAGGCCGTTTTAATTTTTAC ->HWI-EAS91_1_306UPAAXX:6:1:1090:210 -GGAGAGCGCCAACGGCGTCCATCTCGAAGGAGTCGC ->HWI-EAS91_1_306UPAAXX:6:1:589:96 -GGCGGCCCCATCAGGGTTAGGAACATTAGAGCCTTG ->HWI-EAS91_1_306UPAAXX:6:1:1477:1231 -TAGGAACATTAGAGCCTTGAATGGCAGATTTAATAC ->HWI-EAS91_1_306UPAAXX:6:1:707:1076 -TCTGACGTTCGTGATGAGTTTGTATCTTTTTCTTTG ->HWI-EAS91_1_306UPAAXX:6:1:749:1715 -GAACATAATAAGCAATGACGGCAGCAATAAACTCAA ->HWI-EAS91_1_306UPAAXX:6:1:1738:1884 -GCTCACCTTTAGCATCAACAGGCCACAACCAACCAG ->HWI-EAS91_1_306UPAAXX:6:1:1160:1088 -TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC ->HWI-EAS91_1_306UPAAXX:6:1:517:119 -GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT ->HWI-EAS91_1_306UPAAXX:6:1:1472:716 -TGGTAATGGTGGTTTTCTTCATTTCATTCAGTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:281:441 -GAGCAGTAGACTCCTTCTGTTGATAAGCAAGCATCT ->HWI-EAS91_1_306UPAAXX:6:1:1101:324 -AATACCATCAGCTTTACCGTCTTTCCAGAAATTGTT ->HWI-EAS91_1_306UPAAXX:6:1:1225:1494 -TTCTCAAATCCGGCGTCAACCATACCAGCAGAGGAA ->HWI-EAS91_1_306UPAAXX:6:1:1509:1025 -TTCTTGCTGCCGAGGGTCGCAAGGCTATTGTTTCAC ->HWI-EAS91_1_306UPAAXX:6:1:592:510 -GATACCAATAAAATCCCTAAGCATTTGTTTCTGGTT ->HWI-EAS91_1_306UPAAXX:6:1:324:1729 -GAACAAAGAAACGCGGCACAGAATGTTTATAGGTCT ->HWI-EAS91_1_306UPAAXX:6:1:128:1925 -GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC ->HWI-EAS91_1_306UPAAXX:6:1:786:893 -TACGGGGAAGGACGTCAATAGTCACACAGTCCTTGC ->HWI-EAS91_1_306UPAAXX:6:1:248:955 -GCTACAATGTGCTCCCCCAACTTGATATTAATAACA ->HWI-EAS91_1_306UPAAXX:6:1:388:1127 -GATATTGGTCGTATGGTTCTTGCTGCCTAGTGTCTC ->HWI-EAS91_1_306UPAAXX:6:1:721:1156 -TCTGGTTGGTTGTGGCCTTTTTATGCTAAATGTTAG ->HWI-EAS91_1_306UPAAXX:6:1:1564:1468 -TTACTTTTTATGTCCCTCATCGTCACGTTTATGTTG ->HWI-EAS91_1_306UPAAXX:6:1:750:77 -GGCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTA ->HWI-EAS91_1_306UPAAXX:6:1:405:487 -GTTGGATTAAGCACTCCGTGGACAGATTTGTCATTT ->HWI-EAS91_1_306UPAAXX:6:1:836:1204 -TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT ->HWI-EAS91_1_306UPAAXX:6:1:224:1548 -GCTGCCGTCATTGCTTATTATGTTCATCCCTTCAAC ->HWI-EAS91_1_306UPAAXX:6:1:931:1015 -TTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGT ->HWI-EAS91_1_306UPAAXX:6:1:329:579 -GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT ->HWI-EAS91_1_306UPAAXX:6:1:260:1145 -GCTTGCGTTTATGGTACGCTGGACTTTTTGTGATAC ->HWI-EAS91_1_306UPAAXX:6:1:1523:1253 -TTGGTAAAATACTGACCAGCCGTTTGAGCTTGAGTA ->HWI-EAS91_1_306UPAAXX:6:1:326:1271 -GACCACTCGCGATTCAATCATGACTTCGTGATAAAT ->HWI-EAS91_1_306UPAAXX:6:1:213:622 -GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA ->HWI-EAS91_1_306UPAAXX:6:1:274:712 -GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG ->HWI-EAS91_1_306UPAAXX:6:1:1549:627 -TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGT ->HWI-EAS91_1_306UPAAXX:6:1:1714:737 -TCTTTCGTATTCTGGCGTGAAGTCGCCGACTGAATG ->HWI-EAS91_1_306UPAAXX:6:1:760:1217 -TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTT ->HWI-EAS91_1_306UPAAXX:6:1:174:768 -GTTGGCTGACGACCGATTAGAGGCGTTTTTTTATAT ->HWI-EAS91_1_306UPAAXX:6:1:172:1412 -GGTCGGCAGATTGCGATAAACGTTCACATTAAATTT ->HWI-EAS91_1_306UPAAXX:6:1:1393:869 -TTCATCCCGTCAACATTCAAACGGCCTGTCTCATCT ->HWI-EAS91_1_306UPAAXX:6:1:301:481 -GTTATAGATATTCAAATAACCCTGAAACAAATGCTT ->HWI-EAS91_1_306UPAAXX:6:1:648:1093 -TAACGCTGCATGAAGTAATCACGTTCTTGGTCAGTT ->HWI-EAS91_1_306UPAAXX:6:1:1233:591 -TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCT ->HWI-EAS91_1_306UPAAXX:6:1:540:1415 -TTATTAAAGAGATTATTTTTCTCCAGCCACTTATGT ->HWI-EAS91_1_306UPAAXX:6:1:151:1792 -GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA ->HWI-EAS91_1_306UPAAXX:6:1:748:1378 -TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT ->HWI-EAS91_1_306UPAAXX:6:1:1526:1479 -TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC ->HWI-EAS91_1_306UPAAXX:6:1:985:1093 -TAACCGTCTTCTCGTTCTCTAAAAACCATTTTTCTT ->HWI-EAS91_1_306UPAAXX:6:1:480:1378 -TCAACCTCAGCACTAACCTTGCGAGTCATTTCTTTG ->HWI-EAS91_1_306UPAAXX:6:1:903:753 -TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG ->HWI-EAS91_1_306UPAAXX:6:1:1697:1737 -GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT ->HWI-EAS91_1_306UPAAXX:6:1:803:1037 -TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCTACT ->HWI-EAS91_1_306UPAAXX:6:1:1727:1244 -TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT ->HWI-EAS91_1_306UPAAXX:6:1:253:1162 -GCATTTAGTAGCGGTAAAGTTTGACCAAACCATTAT ->HWI-EAS91_1_306UPAAXX:6:1:216:856 -GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT ->HWI-EAS91_1_306UPAAXX:6:1:825:886 -TCCCACAAAGTCCAGCGTACCATAAACGCAAGCCTC ->HWI-EAS91_1_306UPAAXX:6:1:1699:962 -TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT ->HWI-EAS91_1_306UPAAXX:6:1:1210:625 -TCAGATAGTAATCCACGCTCTTTTAAAATGTCAACA ->HWI-EAS91_1_306UPAAXX:6:1:538:616 -TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT ->HWI-EAS91_1_306UPAAXX:6:1:184:1849 -GCTCACCTTTAGCATCAACAGGCCACAACCAACCAG ->HWI-EAS91_1_306UPAAXX:6:1:1636:1103 -TATCTGACTTTTTGTTAACGTATTTAGCCACATAGA ->HWI-EAS91_1_306UPAAXX:6:1:605:223 -GGTTATTTGAATATCTATAACAACTATTTTAAATCG ->HWI-EAS91_1_306UPAAXX:6:1:256:1052 -GGTAAAGGACTTCTTGAAGGTACGTTGCAGTCTGGC ->HWI-EAS91_1_306UPAAXX:6:1:300:1515 -GCCATGATGGTGGTTATTATACCGTCAAGGACTTTT ->HWI-EAS91_1_306UPAAXX:6:1:1684:1320 -TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA ->HWI-EAS91_1_306UPAAXX:6:1:1186:895 -TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT ->HWI-EAS91_1_306UPAAXX:6:1:1463:754 -TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT ->HWI-EAS91_1_306UPAAXX:6:1:808:1053 -TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG ->HWI-EAS91_1_306UPAAXX:6:1:960:1218 -TTTCTAATGTCGTCACTGATGCTGCTTCTGTTGTTT ->HWI-EAS91_1_306UPAAXX:6:1:521:1646 -GGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCA ->HWI-EAS91_1_306UPAAXX:6:1:289:1885 -GCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAG ->HWI-EAS91_1_306UPAAXX:6:1:471:170 -GGTCAGTTCCATCAACATCATAGCCAGATGCCCAGA ->HWI-EAS91_1_306UPAAXX:6:1:828:754 -TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT ->HWI-EAS91_1_306UPAAXX:6:1:924:1679 -TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT ->HWI-EAS91_1_306UPAAXX:6:1:837:901 -TGCATTTTAGTAAGCTCTTTTTGATTCTCAAATCCG ->HWI-EAS91_1_306UPAAXX:6:1:543:16 -GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC ->HWI-EAS91_1_306UPAAXX:6:1:1482:578 -TCTTTAGCTCCTAGACCTTTAGCAGCAAGGTCCATA ->HWI-EAS91_1_306UPAAXX:6:1:1254:1668 -TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT ->HWI-EAS91_1_306UPAAXX:6:1:1402:898 -TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC ->HWI-EAS91_1_306UPAAXX:6:1:764:1534 -TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT ->HWI-EAS91_1_306UPAAXX:6:1:681:1079 -TGGCGAATAAGTACGCGTTCTTGCAAATCACCAGAA ->HWI-EAS91_1_306UPAAXX:6:1:672:1350 -TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT ->HWI-EAS91_1_306UPAAXX:6:1:1266:493 -TGACCAGCCGTTTGAGCTTGAGTAAGCATTTGGCGC ->HWI-EAS91_1_306UPAAXX:6:1:118:238 -GACGGTATAATAACCACCATCATGGCGACCATTCAA ->HWI-EAS91_1_306UPAAXX:6:1:699:433 -TTATTGCCCGGCGTACGGGGAAGGACGTCAATAGTC ->HWI-EAS91_1_306UPAAXX:6:1:708:1387 -TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC ->HWI-EAS91_1_306UPAAXX:6:1:498:1085 -TTATGATAATCCCAATGCTTTGCGTGACTATTTTCT ->HWI-EAS91_1_306UPAAXX:6:1:1101:1301 -TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAT ->HWI-EAS91_1_306UPAAXX:6:1:261:213 -GAATGGTCGCCATGATGGTGGTTATTATACCGTCAC ->HWI-EAS91_1_306UPAAXX:6:1:1287:1267 -TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGT ->HWI-EAS91_1_306UPAAXX:6:1:744:331 -TTAATGGATGAATTGGCACAATGCTACAATGTGCTC ->HWI-EAS91_1_306UPAAXX:6:1:614:814 -TGTCAGCGTCATAAGAGGTTTTACCTCCAAATGAAG ->HWI-EAS91_1_306UPAAXX:6:1:1362:1063 -TAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAG ->HWI-EAS91_1_306UPAAXX:6:1:1238:1508 -TCAACTAACGATTCTGTCAAAAACTGACGCGTTGGT ->HWI-EAS91_1_306UPAAXX:6:1:904:1130 -TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT ->HWI-EAS91_1_306UPAAXX:6:1:465:216 -GACCATGCCGCTTTTCTTGGCACGATTAACCCTTAT ->HWI-EAS91_1_306UPAAXX:6:1:844:628 -TAATGTCAATAGATGTGGTAGAAGTCGTCATTTGGC ->HWI-EAS91_1_306UPAAXX:6:1:684:1444 -TATCCCACAAAGTCCAGCGTACCATAAACGCAAGCC ->HWI-EAS91_1_306UPAAXX:6:1:515:1373 -TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT ->HWI-EAS91_1_306UPAAXX:6:1:764:1667 -TTGAGTTCGATAATGGTGATATGTATGTTGACGTCC ->HWI-EAS91_1_306UPAAXX:6:1:1722:598 -TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTCT ->HWI-EAS91_1_306UPAAXX:6:1:670:1188 -TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT ->HWI-EAS91_1_306UPAAXX:6:1:1682:1705 -TAGCCACATAGAAACCAACAGCCATATAACTGGTAG ->HWI-EAS91_1_306UPAAXX:6:1:1008:1616 -TCCTTTACTTGTCATGCGCTCTAATCTCTGTGCATC ->HWI-EAS91_1_306UPAAXX:6:1:490:1220 -TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCT ->HWI-EAS91_1_306UPAAXX:6:1:891:1437 -TAATGGTGATATGTATGTTTACGTCCATAAGGCTGT ->HWI-EAS91_1_306UPAAXX:6:1:1310:321 -TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGT ->HWI-EAS91_1_306UPAAXX:6:1:827:1597 -TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC ->HWI-EAS91_1_306UPAAXX:6:1:1062:1158 -TAGAGTCAATAGCAAGGCCACGACGCAATGGAGAAA ->HWI-EAS91_1_306UPAAXX:6:1:1419:208 -TGGCGCATAATCTCGGAAACCTGCTGTTGCTTGGAA ->HWI-EAS91_1_306UPAAXX:6:1:691:1018 -AAATATCAACCACACCAGAAGCAGCATCAGTGACGA ->HWI-EAS91_1_306UPAAXX:6:1:374:113 -GATAAAGCTGTTGCCGATACTTGGAACAATTTCTGT ->HWI-EAS91_1_306UPAAXX:6:1:1720:784 -TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC ->HWI-EAS91_1_306UPAAXX:6:1:1424:1394 -ATAAAAATGATTGGCGTATCCAACCTGCAGAGTTTT ->HWI-EAS91_1_306UPAAXX:6:1:1063:1760 -TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT ->HWI-EAS91_1_306UPAAXX:6:1:1235:729 -TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA ->HWI-EAS91_1_306UPAAXX:6:1:167:1507 -TAGTGTTATTAATATCAAGTTTTTGGAGCACATTGT ->HWI-EAS91_1_306UPAAXX:6:1:717:1569 -TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT ->HWI-EAS91_1_306UPAAXX:6:1:610:765 -TTCAGCGCCTTCCATGATGAGACAGGCCGTTTGAAT ->HWI-EAS91_1_306UPAAXX:6:1:663:380 -TAAACATTCTGTGCCGCGTTTCTTTGTTCCTTATCT ->HWI-EAS91_1_306UPAAXX:6:1:790:1358 -TTATCACCTTATTGAAGGCTTATCATTCATTTAGGT ->HWI-EAS91_1_306UPAAXX:6:1:965:1633 -TAGATGTGGTAGAAGTCGTCATTTGGCGAGAAAGCT ->HWI-EAS91_1_306UPAAXX:6:1:673:319 -TTCTTGCAAATCACCAGAAGGCGGTTCCTGAATGAT ->HWI-EAS91_1_306UPAAXX:6:1:684:371 -TAGCGGTAAAGTTAGACCAAACCATGAAACCAACAT ->HWI-EAS91_1_306UPAAXX:6:1:1147:1444 -ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC ->HWI-EAS91_1_306UPAAXX:6:1:983:678 -ATACCTGGTCTTTCGTATTCTGGCGTGAAGTCGCCG ->HWI-EAS91_1_306UPAAXX:6:1:1608:1119 -TCACGCGGCGGCAAGTTGCCATACAAAACAGGGTCG ->HWI-EAS91_1_306UPAAXX:6:1:1048:1193 -TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCAT ->HWI-EAS91_1_306UPAAXX:6:1:1265:1429 -ATATTTTTCATGGTATTGATAAAGCTGTTGCCGATT ->HWI-EAS91_1_306UPAAXX:6:1:1607:1677 -TGTTGCTTGGAAAGATTGGTGTTTTCCATAATAGAC ->HWI-EAS91_1_306UPAAXX:6:1:1087:1421 -ACGAACGTCAGAAGCAGCCTTATGGCCGTCAACATC ->HWI-EAS91_1_306UPAAXX:6:1:324:490 -GCACCAAACATAAATCACCTCACTTAAGTGGCTGGG ->HWI-EAS91_1_306UPAAXX:6:1:1596:614 -TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGT ->HWI-EAS91_1_306UPAAXX:6:1:343:83 -GTTACGCAGTTTTGCCGCAAGCTGGCTGCTGTACGC ->HWI-EAS91_1_306UPAAXX:6:1:203:667 -GCATGAATGTGCTTAATAGAGGCCAAGGCGGTCTAG ->HWI-EAS91_1_306UPAAXX:6:1:34:480 -GGCAAGTTGCCATACAAAACAGGGTCGCCAGCAATT ->HWI-EAS91_1_306UPAAXX:6:1:606:1743 -TAGCGACAGCTTGGTTTTTAGTGAGTTGTTCCATTC ->HWI-EAS91_1_306UPAAXX:6:1:254:1391 -TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG ->HWI-EAS91_1_306UPAAXX:6:1:1568:1750 -TAACCAGTAGTGTTAACAGTCGGGAGAGGAGTGGCT ->HWI-EAS91_1_306UPAAXX:6:1:1538:869 -TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA ->HWI-EAS91_1_306UPAAXX:6:1:255:38 -GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT ->HWI-EAS91_1_306UPAAXX:6:1:1543:1555 -TAAAACGCCTCTAATCGGTCGTCAGCCAACGTGAGG ->HWI-EAS91_1_306UPAAXX:6:1:1365:733 -AGAATCAGCGGTATGGCTCTTCTCCTTTTTTCGCTT ->HWI-EAS91_1_306UPAAXX:6:1:1604:943 -TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT ->HWI-EAS91_1_306UPAAXX:6:1:1574:1632 -TCAGTATGCAAATTAGCATAAGCAGCTTGCAGACCC ->HWI-EAS91_1_306UPAAXX:6:1:565:1799 -TCTTGGTCAGTATGCAAATTAGCATAAGCAGCTTGC ->HWI-EAS91_1_306UPAAXX:6:1:1004:380 -TATTGACTCTACTGTAGACATTTTTACTTTTTATTT ->HWI-EAS91_1_306UPAAXX:6:1:1345:965 -ATTCAAAGGATAAACATCATAGGCAGTCGGGAGGGT ->HWI-EAS91_1_306UPAAXX:6:1:1704:756 -TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG ->HWI-EAS91_1_306UPAAXX:6:1:310:1346 -TATAACGTTGACGATGTAGCTTTAGTTTTCTTTAAA ->HWI-EAS91_1_306UPAAXX:6:1:900:1858 -TTTACCGCTTCGGCGTTATAACCTCACACTCAATCT ->HWI-EAS91_1_306UPAAXX:6:1:1250:1741 -TAAATCCAAAACGGCAGAAGCCTGAATGAGCTTAAT ->HWI-EAS91_1_306UPAAXX:6:1:1170:1317 -TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC ->HWI-EAS91_1_306UPAAXX:6:1:149:1896 -GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC ->HWI-EAS91_1_306UPAAXX:6:1:1504:494 -TGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCC ->HWI-EAS91_1_306UPAAXX:6:1:395:256 -GTCCATATCTGACTTTTTGTTAACGTATTTATCCAC ->HWI-EAS91_1_306UPAAXX:6:1:1110:1109 -ACCGCTTCGGCGTTATAACCTCACACTCAATCTTTT ->HWI-EAS91_1_306UPAAXX:6:1:895:649 -TTCTGCACGTAATTTTTGACGCACGTTTTCTTCTGC ->HWI-EAS91_1_306UPAAXX:6:1:827:1378 -TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG ->HWI-EAS91_1_306UPAAXX:6:1:1051:1587 -TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG ->HWI-EAS91_1_306UPAAXX:6:1:1656:1549 -AACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCCAT ->HWI-EAS91_1_306UPAAXX:6:1:366:150 -GGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAA ->HWI-EAS91_1_306UPAAXX:6:1:955:1792 -ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA ->HWI-EAS91_1_306UPAAXX:6:1:1340:1403 -ATAAAATGCACCGCATGGAAATGAAGACGGCCATTA ->HWI-EAS91_1_306UPAAXX:6:1:1693:1017 -TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGT ->HWI-EAS91_1_306UPAAXX:6:1:1099:1572 -AATTTTTACCGCTTCGGCGTTATAACCTCACACTCA ->HWI-EAS91_1_306UPAAXX:6:1:218:1148 -TATGCAAATTAGCATAAGCAGCTTGCAGACCCATAT ->HWI-EAS91_1_306UPAAXX:6:1:403:614 -TGGTGCTGATGCTTCCTCTGCTGGTATGGTTTACGC ->HWI-EAS91_1_306UPAAXX:6:1:1651:646 -TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC ->HWI-EAS91_1_306UPAAXX:6:1:1566:499 -TGCGGTGCATTTTATGCGGACACTTCCTACAGGTAG ->HWI-EAS91_1_306UPAAXX:6:1:825:951 -ACAGGCCGTTTGAATGTTTACGGGGTGTACATAATA ->HWI-EAS91_1_306UPAAXX:6:1:1745:1865 -TTAACTTCTGCGTCATGGAAGCGATAAAACTCTGCG ->HWI-EAS91_1_306UPAAXX:6:1:973:1992 -TAGTAATTCCTGCTTTATCAAGATAATTTTTCGACT ->HWI-EAS91_1_306UPAAXX:6:1:171:1653 -TAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGT ->HWI-EAS91_1_306UPAAXX:6:1:397:363 -TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT ->HWI-EAS91_1_306UPAAXX:6:1:1336:1155 -ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG ->HWI-EAS91_1_306UPAAXX:6:1:685:629 -AGTATGCAAATTAGCATAAGCAGCTTGCAGACCCAT ->HWI-EAS91_1_306UPAAXX:6:1:1409:510 -ACATAATAAGCAATGACGGCAGCAATAAACTCAACA ->HWI-EAS91_1_306UPAAXX:6:1:1631:998 -AACCATCAGCATGAGCCTGTCGCATTGCATTCATCC ->HWI-EAS91_1_306UPAAXX:6:1:260:1698 -TTATTATGTTCATCCCGTCAACATTCAAACTGCCTT ->HWI-EAS91_1_306UPAAXX:6:1:578:971 -TTAACGCTACTAAATTCCGCGGATTGGTTTCGTTGT ->HWI-EAS91_1_306UPAAXX:6:1:1613:642 -ATAGAAATTTCACGCGGCGGCAAGTTGCCATACAAA ->HWI-EAS91_1_306UPAAXX:6:1:237:650 -GACGGTATAATAACCACCATCATGGCGACCATTCAA ->HWI-EAS91_1_306UPAAXX:6:1:1100:1875 -TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG ->HWI-EAS91_1_306UPAAXX:6:1:352:32 -GTACCATAAACGCAAGCCTCAACGCAGCGACGAGCC ->HWI-EAS91_1_306UPAAXX:6:1:443:229 -GCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAAC ->HWI-EAS91_1_306UPAAXX:6:1:1131:731 -AGCAGTCGGCGTGTGAATCATTAGCCTTGCGACCCT ->HWI-EAS91_1_306UPAAXX:6:1:133:1089 -AAGGTTAGTGCTGAGGTTGACTTAGTTCATCATCAA ->HWI-EAS91_1_306UPAAXX:6:1:65:1307 -TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT ->HWI-EAS91_1_306UPAAXX:6:1:905:1493 -TCAGCTTTACCGTCTTTCCAGAAATTGTTCCAAGTT ->HWI-EAS91_1_306UPAAXX:6:1:733:540 -TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAT ->HWI-EAS91_1_306UPAAXX:6:1:161:1707 -TAATGTCGTCACTGATGCTGCTTCTGTTGTTGTTTT ->HWI-EAS91_1_306UPAAXX:6:1:380:1861 -TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC ->HWI-EAS91_1_306UPAAXX:6:1:1761:566 -TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAT ->HWI-EAS91_1_306UPAAXX:6:1:1486:651 -TGGCGGTATTTCTTCTTCTCTTTCTTGTTGCGCCCT ->HWI-EAS91_1_306UPAAXX:6:1:508:1380 -TCCATCAACATCATAGCCAGATGCCCAGAGATTAGA ->HWI-EAS91_1_306UPAAXX:6:1:1763:855 -TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC ->HWI-EAS91_1_306UPAAXX:6:1:1553:553 -TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA ->HWI-EAS91_1_306UPAAXX:6:1:1424:507 -TCCACTGCAACAACTGAACGGACTGGAAACACTGGT ->HWI-EAS91_1_306UPAAXX:6:1:988:135 -TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC ->HWI-EAS91_1_306UPAAXX:6:1:810:1918 -TTTTCATCCCGAAGTTGCGGCTCATTCTGATTCTGT ->HWI-EAS91_1_306UPAAXX:6:1:588:559 -TCTGGTTGAACGGCGTCGCGTCGTAACCCAGCTTGG ->HWI-EAS91_1_306UPAAXX:6:1:1264:1214 -ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC ->HWI-EAS91_1_306UPAAXX:6:1:1000:1475 -TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG ->HWI-EAS91_1_306UPAAXX:6:1:1389:160 -TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT ->HWI-EAS91_1_306UPAAXX:6:1:422:1296 -TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGT ->HWI-EAS91_1_306UPAAXX:6:1:1273:856 -TAGCCATAGCACCAGAAACAAAACTAGGGGCGGCCT ->HWI-EAS91_1_306UPAAXX:6:1:450:969 -TGTTTTCCATAATAGACGCAACGCGAGCAGTAGACT ->HWI-EAS91_1_306UPAAXX:6:1:1202:828 -ATCGTCAACGTTATATTTTGATAGTTTGACGTTTAT ->HWI-EAS91_1_306UPAAXX:6:1:1721:1800 -GGGTTAGGGACATTAGAGCCTTGACTGACTGAGTTT ->HWI-EAS91_1_306UPAAXX:6:1:228:2019 -TTGAGTAAGCATTTGGCGCATAATCTCGGAAACCTG ->HWI-EAS91_1_306UPAAXX:6:1:1579:1214 -ACGTTTGGTCAGTTCCATCAACATCATAGCCAGATG ->HWI-EAS91_1_306UPAAXX:6:1:429:1055 -TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTCTTT ->HWI-EAS91_1_306UPAAXX:6:1:1359:1689 -AAGAGCAGAAGCAATACCGCCAGCAATAGCACCAAA ->HWI-EAS91_1_306UPAAXX:6:1:1474:1056 -TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC ->HWI-EAS91_1_306UPAAXX:6:1:105:1818 -TTGGGGATTGAGAAAGAGTAGAAATGCCACAAGCCT ->HWI-EAS91_1_306UPAAXX:6:1:208:1538 -TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA ->HWI-EAS91_1_306UPAAXX:6:1:1361:1623 -AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA ->HWI-EAS91_1_306UPAAXX:6:1:595:1670 -TGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACA ->HWI-EAS91_1_306UPAAXX:6:1:6:1885 -TCTAATGTCGTCACTGATGCTGCTTCTGGTGTGTTT ->HWI-EAS91_1_306UPAAXX:6:1:706:1085 -TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG ->HWI-EAS91_1_306UPAAXX:6:1:1307:825 -AGCGGTAAAGTTAGACCAAACCATGAAACCAACATA ->HWI-EAS91_1_306UPAAXX:6:1:762:802 -TGGCATTAACACCATCCTTCATGAACTTAATCCACT ->HWI-EAS91_1_306UPAAXX:6:1:1657:506 -TTGCGACCCTCGGCAGCAAGAACCATACGACCAATT ->HWI-EAS91_1_306UPAAXX:6:1:184:811 -TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC ->HWI-EAS91_1_306UPAAXX:6:1:1469:1718 -TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTTT ->HWI-EAS91_1_306UPAAXX:6:1:815:1640 -TGGCGGCGATTGCGTACCCGACGACCCAAATTAGGG ->HWI-EAS91_1_306UPAAXX:6:1:1580:1388 -AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG ->HWI-EAS91_1_306UPAAXX:6:1:1617:1554 -TACGGGGAAGGACGTCAATAGTCACACAGTCCTTGA ->HWI-EAS91_1_306UPAAXX:6:1:1544:431 -TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA ->HWI-EAS91_1_306UPAAXX:6:1:1604:1541 -TCAGTGACGACATTAGAAATATCCTTTGCAGTAGCG ->HWI-EAS91_1_306UPAAXX:6:1:1485:741 -ATCAAACGCTGAATAGTAAAGCCTCTACGCGATTTC ->HWI-EAS91_1_306UPAAXX:6:1:1226:393 -TGCCACAAGCCTCAATAGCAGGTTTAAGAGCCTCGA ->HWI-EAS91_1_306UPAAXX:6:1:1506:973 -ATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCAT ->HWI-EAS91_1_306UPAAXX:6:1:890:1838 -TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC ->HWI-EAS91_1_306UPAAXX:6:1:453:1527 -TAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACT ->HWI-EAS91_1_306UPAAXX:6:1:1056:570 -ACATTGTAGCATTGTGCCAATTCATCCATTAACTTC ->HWI-EAS91_1_306UPAAXX:6:1:1736:74 -TATCCGAAAGTGTTAACTTCTGCGTCATGGAAGCGT ->HWI-EAS91_1_306UPAAXX:6:1:169:1896 -GTATGCAAATTAGCATAAGCAGCTTGCAGACCCATA ->HWI-EAS91_1_306UPAAXX:6:1:259:949 -TGAGGATAAATTATGTCTAATATTCAAACTTGCTCC ->HWI-EAS91_1_306UPAAXX:6:1:1205:893 -ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT ->HWI-EAS91_1_306UPAAXX:6:1:732:1335 -TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT ->HWI-EAS91_1_306UPAAXX:6:1:667:664 -TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG ->HWI-EAS91_1_306UPAAXX:6:1:535:587 -TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT ->HWI-EAS91_1_306UPAAXX:6:1:412:446 -GTGTGGTTGATATTTTTCATGGTATTGATAAAGCTT ->HWI-EAS91_1_306UPAAXX:6:1:507:1599 -TTGCTGGCGGTTTTTCTTTTTTTTTTTTTTTTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:575:1818 -TAAAATGCACCGCATGGAAATGAAGACGGCCATTAG ->HWI-EAS91_1_306UPAAXX:6:1:1568:1428 -ACCAGTTATATGGCTGGTTGTTTTTTTTTTTTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:1417:982 -AACAAGAGAATCTCTACCATGAACAAAATGTGACTC ->HWI-EAS91_1_306UPAAXX:6:1:280:1340 -GGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTTT ->HWI-EAS91_1_306UPAAXX:6:1:966:144 -TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT ->HWI-EAS91_1_306UPAAXX:6:1:1391:1987 -TAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGT ->HWI-EAS91_1_306UPAAXX:6:1:1280:278 -ATGGAAATGAAGACGGCCATTAGCTGTACCATACTC ->HWI-EAS91_1_306UPAAXX:6:1:631:858 -TGATATTGGTCGTATGGTTCTTGCTTCCGTGGGTCT ->HWI-EAS91_1_306UPAAXX:6:1:518:573 -TTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGT ->HWI-EAS91_1_306UPAAXX:6:1:54:981 -TTGACATTTTAAAAGAGCGTGGATTACTATCTGATT ->HWI-EAS91_1_306UPAAXX:6:1:218:1165 -TATTGACTCTACTGTAGACATTTTTACTTTTTATTT ->HWI-EAS91_1_306UPAAXX:6:1:1727:1530 -TCAACGCAGCGACGAGCACGAGAGCGGTCAGTAGCA ->HWI-EAS91_1_306UPAAXX:6:1:519:657 -TGAACAGCATCGGACTCAGATAGTAATCCACGCTCT ->HWI-EAS91_1_306UPAAXX:6:1:939:967 -ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC ->HWI-EAS91_1_306UPAAXX:6:1:299:1060 -TATAACTGGTAGCTTTAAGCGGCTCACCTTTAGCAT ->HWI-EAS91_1_306UPAAXX:6:1:438:665 -TAATTCGTAAACAAGCAGTAGTAATTCCTGCTTTAT ->HWI-EAS91_1_306UPAAXX:6:1:1303:1971 -AGCATTGTGCCAATTCATCCATTAACTTCTCAGTAA ->HWI-EAS91_1_306UPAAXX:6:1:214:1264 -TCAGCACCAACAGAAACAACCTGATTAGCGGCGTTG ->HWI-EAS91_1_306UPAAXX:6:1:1454:1423 -AACGGAAAACATCCTTCATAGAAATTTCACGCGGCG ->HWI-EAS91_1_306UPAAXX:6:1:1633:340 -TTCCATAATAGACGCAACGCGAGCAGTAGACTCCTT ->HWI-EAS91_1_306UPAAXX:6:1:671:1196 -ATACGAAAAGACAGAATCTCTTCCAAGAGCTTGATG
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/chrM_human.fa Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,333 @@ +>chrM +GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCAT +TTGGTATTTTCGTCTGGGGGGTATGCACGCGATAGCATTGCGAGACGCTG +GAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATC +CTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTA +AAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAAT +GTCTGCACAGCCACTTTCCACACAGACATCATAACAAAAAATTTCCACCA +AACCCCCCCTCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCA +AACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTT +TATCTTTTGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACATT +ATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCCGC +CCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCA +ACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAA +GCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAAAT +AGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAA +GCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGA +ACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCAC +ACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTT +AACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGC +GGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTA +GATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACT +CCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACAC +ACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCC +TAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGC +CACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGG +AGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTC +AGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAA +GCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTG +GCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTAT +GAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTAAGAGTAGAGTGC +TTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTC +AAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGA +GGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAA +CCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCA +ACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCT +TACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCG +ATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATG +AAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCT +GCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGA +CCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGT +CTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC +CGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTA +AATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTC +CAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGT +AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA +GCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGA +ACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATG +TTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGA +TTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAAC +AAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGA +AAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTA +CCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCA +GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAaaggtagcata +atcacttgttccttaaatagggacctgtatgaatggctccacgagggttc +agctgtctcttacttttaaccagtgaaattgacctgcccgtgaagaggcg +ggcataacacagcaagacgagaagaccctatggagctttaatttaTTAAT +GCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT +AAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGT +ACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCC +AATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCT +ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA +GGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT +AAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCT +ATCTACNTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCT +ACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATT +ATACCCACACCCACCCAAGAACAGGGTTTgttaagatggcagagcccggt +aatcgcataaaacttaaaactttacagtcagaggttcaattcctcttctt +aacaacaTACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAAT +CGCAATGGCATTCCTAATGCTTACCGAACGAAAAATTCTAGGCTATATAC +AACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTACAACCC +TTCGCTGACGCCATAAAACTCTTCACCAAAGAGCCCCTAAAACCCGCCAC +ATCTACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACCATCG +CTCTTCTACTATGAACCCCCCTCCCCATACCCAACCCCCTGGTCAACCTC +AACCTAGGCCTCCTATTTATTCTAGCCACCTCTAGCCTAGCCGTTTACTC +AATCCTCTGATCAGGGTGAGCATCAAACTCAAACTACGCCCTGATCGGCG +CACTGCGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATC +ATTCTACTATCAACATTACTAATAAGTGGCTCCTTTAACCTCTCCACCCT +TATCACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTGG +CCATAATATGATTTATCTCCACACTAGCAGAGACCAACCGAACCCCCTTC +GACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATCGAATA +CGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTA +TTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGAC +GCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACT +TCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCGCT +ACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTA +GCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGCATTCC +CCCTCAAACCTAAGAAATATGTCTGATAAAAGAGTTACTTTGATAGAGTA +AATAATAGGAGCTTAAACCCCCTTATTTctaggactatgagaatcgaacc +catccctgagaatccaaaattctccgtgccacctatcacaccccatccta +AAGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGG +TTATACCCTTCCCGTACTAATTAATCCCCTGGCCCAACCCGTCATCTACT +CTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTT +TTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCT +AACCAAAAAAATAAACCCTCGTTCCACAGAAGCTGCCATCAAGTATTTCC +TCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAAC +AATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTC +ATCATTAATAATCATAATAGCTATAGCAATAAAACTAGGAATAGCCCCCT +TTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGC +CTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTCAATCATATACCA +AATCTCTCCCTCACTAAACGTAAGCCTTCTCCTCACTCTCTCAATCTTAT +CCATCATAGCAGGCAGTTGAGGTGGATTAAACCAAACCCAGCTACGCAAA +ATCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATAGCAGTTCT +ACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCC +TAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACC +CTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAAT +TCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTT +TGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCATC +ATCCCCACCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTACCT +ACGCCTAATCTACTCCACCTCAATCACACTACTCCCCATATCTAACAACG +TAAAAATAAAATGACAGTTTGAACATACAAAACCCACCCCATTCCTCCCC +ACACTCATCGCCCTTACCACGCTACTCCTACCTATCTCCCCTTTTATACT +AATAATCTTATAGAAATTTAGGTTAAATACAGACCAAGAGCCTTCAAAGC +CCTCAGTAAGTTGCAATACTTAATTTCTGTAACAGCTAAGGACTGCAAAA +CCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAG +CCCTTACTAGACCAATGGGACTTAAACCCACAAACACTTAGTTAACAGCT +AAGCACCCTAATCAACTGGCTTCAATCTACTTCTCCCGCCGCCGGGAAAA +AAGGCGGGAGAAGCCCCGGCAGGTTTGAAGCTGCTTCTTCGAATTTGCAA +TTCAATATGAAAATCACCTCGGAGCTGGTAAAAAGAGGCCTAACCCCTGT +CTTTAGATTTACAGTCCAATGCTTCACTCAGCCATTTTACCTCACCCCCA +CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG +AACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTC +TAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGT +AACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAAT +CTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAG +TTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAAC +ATAAGCTTCTGACTCTTACCTCCCTCTCTCCTACTCCTGCTCGCATCTGC +TATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAG +CAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCC +TTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCAC +AACAATTATCAATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCC +TCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTC +CCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAA +CACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAAC +ACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCA +GGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGA +ACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCC +TAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTA +GACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCAC +CGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGA +AATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACC +GTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGT +ACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAA +TAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTA +TTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTAT +CATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCC +TATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACA +TGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAAT +ATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCC +TAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCC +CCACCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAaaa +aaggaaggaatcgaaccccccaaagctggtttcaagccaaccccatggcc +tccatgactttttcAAAAAGGTATTAGAAAAACCATTTCATAACTTTGTC +AAAGTTAAATTATAGGCTAAATCCTATATATCTTAATGGCACATGCAGCG +CAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCAC +CTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCC +TGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATC +TCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCAT +CCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACG +AGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCACCAATGGTAC +TGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACAT +ACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTG +ACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACA +TCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAAC +AGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTACACGAC +CGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGT +TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGG +GCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCCACTGT +AAAGCTAACTTAGCATTAACCTTTTAAGTTAAAGATTAAGAGAACCAACA +CCTCTTTACAGTGAAATGCCCCAACTAAATACTACCGTATGGCCCACCAT +AATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAAATAT +TAAACACAAACTACCACCTACCTCCCTCACCAAAGCCCATAAAAATAAAA +AATTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGCTTCA +TTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCT +ATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGAC +TAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGATA +ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT +AATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCAT +TTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTA +TGAGCGGGCACAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCT +AGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAG +TTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTA +CGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGG +AAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCA +TCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTA +ATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACGACAA +CACATAATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCC +ATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAG +CCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTA +ACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAG +CACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACG +GGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTC +TGAGCCTTTTACCACTCCAGCCTAGCCCCTACCCCCCAATTAGGAGGGCA +CTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCC +TAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCAC +CATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTAT +TACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACT +TCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTT +GTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCT +CACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATC +ACTTTGGCTTCGAAGCCGCCGCCTGATACTGGCATTTTGTAGATGTGGTT +TGACTATTTCTGTATGTCTCCATCTATTGATGAGGGTCTTACTCTTTTAG +TATAAATAGTACCGTTAACTTCCAATTAACTAGTTTTGACAACATTCAAA +AAAGAGTAATAAACTTCGCCTTAATTTTAATAATCAACACCCTCCTAGCC +TTACTACTAATAATTATTACATTTTGACTACCACAACTCAACGGCTACAT +AGAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCC +GCGTCCCTTTCTCCATAAAATTCTTCTTAGTAGCTATTACCTTCTTATTA +TTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCATGAGCCCTACAAAC +AACTAACCTGCCACTAATAGTTATGTCATCCCTCTTATTAATCATCATCC +TAGCCCTAAGTCTGGCCTATGAGTGACTACAAAAAGGATTAGACTGAACC +GAATTGGTATATAGTTTAAACAAAACGAATGATTTCGACTCATTAAATTA +TGATAATCATATTTACCAAATGCCCCTCATTTACATAAATATTATACTAG +CATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCTCATA +TCCTCCCTACTATGCCTAGAAGGAATAATACTATCGCTGTTCATTATAGC +TACTCTCATAACCCTCAACACCCACTCCCTCTTAGCCAATATTGTGCCTA +TTGCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTA +CTAGTCTCAATCTCCAACACATATGGCCTAGACTACGTACATAACCTAAA +CCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTACTACCACT +GACATGACTTTCCAAAAAACACATAATTTGAATCAACACAACCACCCACA +GCCTAATTATTAGCATCATCCCTCTACTATTTTTTAACCAAATCAACAAC +AACCTATTTAGCTGTTCCCCAACCTTTTCCTCCGACCCCCTAACAACCCC +CCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCC +AACGCCACTTATCCAGTGAACCACTATCACGAAAAAAACTCTACCTCTCT +ATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGA +ACTAATCATATTTTATATCTTCTTCGAAACCACACTTATCCCCACCTTGG +CTATCATCACCCGATGAGGCAACCAGCCAGAACGCCTGAACGCAGGCACA +TACTTCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACT +AATTTACACTCACAACACCCTAGGCTCACTAAACATTCTACTACTCACTC +TCACTGCCCAAGAACTATCAAACTCCTGAGCCAACAACTTAATATGACTA +GCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACTT +ATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAATAGTAC +TTGCCGCAGTACTCTTAAAACTAGGCGGCTATGGTATAATACGCCTCACA +CTCATTCTCAACCCCCTGACAAAACACATAGCCTACCCCTTCCTTGTACT +ATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAG +ACCTAAAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGTA +GTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCAT +TCTCATAATCGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAA +ACTCAAACTACGAACGCACTCACAGTCGCATCATAATCCTCTCTCAAGGA +CTTCAAACTCTACTCCCACTAATAGCTTTTTGATGACTTCTAGCAAGCCT +CGCTAACCTCGCCTTACCCCCCACTATTAACCTACTGGGAGAACTCTCTG +TGCTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTTACAGGA +CTCAACATACTAGTCACAGCCCTATACTCCCTCTACATATTTACCACAAC +ACAATGGGGCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCA +CACGAGAAAACACCCTCATGTTCATACACCTATCCCCCATTCTCCTCCTA +TCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATAGTTT +AACCAAAACATCAGATTGTGAATCTGACAACAGAGGCTTACGACCCCTTA +TTTACCGAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAAC +AACATGGCTTTCTCAACTTTTAAAGGATAACAGCTATCCATTGGTCTTAG +GCCCCAAAAATTTTGGTGCAACTCCAAATAAAAGTAATAACCATGCACAC +TACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCA +CCCTCGTTAACCCTAACAAAAAAAACTCATACCCCCATTATGTAAAATCC +ATTGTCGCATCCACCTTTATTATCAGTCTCTTCCCCACAACAATATTCAT +GTGCCTAGACCAAGAAGTTATTATCTCGAACTGACACTGAGCCACAACCC +AAACAACCCAGCTCTCCCTAAGCTTCAAACTAGACTACTTCTCCATAATA +TTCATCCCTGTAGCATTGTTCGTTACATGGTCCATCATAGAATTCTCACT +GTGATATATAAACTCAGACCCAAACATTAATCAGTTCTTCAAATATCTAC +TCATCTTCCTAATTACCATACTAATCTTAGTTACCGCTAACAACCTATTC +CAACTGTTCATCGGCTGAGAGGGCGTAGGAATTATATCCTTCTTGCTCAT +CAGTTGATGATACGCCCGAGCAGATGCCAACACAGCAGCCATTCAAGCAA +TCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATGA +TTTATCCTACACTCCAACTCATGAGACCCACAACAAATAGCCCTTCTAAA +CGCTAATCCAAGCCTCACCCCACTACTAGGCCTCCTCCTAGCAGCAGCAG +GCAAATCAGCCCAATTAGGTCTCCACCCCTGACTCCCCTCAGCCATAGAA +GGCCCCACCCCAGTCTCAGCCCTACTCCACTCAAGCACTATAGTTGTAGC +AGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCAGAAAATAGCCCAC +TAATCCAAACTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCA +GCAGTCTGCGCCCTTACACAAAATGACATCAAAAAAATCGTAGCCTTCTC +CACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCATCAACCAAC +CACACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTTCAAAGCCATA +CTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAACAAGA +TATTCGAAAAATAGGAGGACTACTCAAAACCATACCTCTCACTTCAACCT +CCCTCACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTCACAGGT +TTCTACTCCAAAGACCACATCATCGAAACCGCAAACATATCATACACAAA +CGCCTGAGCCCTATCTATTACTCTCATCGCTACCTCCCTGACAAGCGCCT +ATAGCACTCGAATAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCC +ACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCCATTAA +ACGCCTGGCAGCCGGAAGCCTATTCGCAGGATTTCTCATTACTAACAACA +TTTCCCCCGCATCCCCCTTCCAAACAACAATCCCCCTCTACCTAAAACTC +ACAGCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAA +CTACCTAACCAACAAACTTAAAATAAAATCCCCACTATGCACATTTTATT +TCTCCAACATACTCGGATTCTACCCTAGCATCACACACCGCACAATCCCC +TATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAGACCT +AACCTGACTAGAAAAGCTATTACCTAAAACAATTTCACAGCACCAAATCT +CCACCTCCATCATCACCTCAACCCAAAAAGGCATAATTAAACTTTACTTC +CTCTCTTTCTTCTTCCCACTCATCCTAACCCTACTCCTAATCACATAACC +TATTCCCCCGAGCAATCTCAATTACAATATATACACCAACAAACAATGTT +CAACCAGTAACTACTACTAATCAACGCCCATAATCATACAAAGCCCCCGC +ACCAATAGGATCCTCCCGAATCAACCCTGACCCCTCTCCTTCATAAATTA +TTCAGCTTCCTACACTATTAAAGTTTACCACAACCACCACCCCATCATAC +TCTTTCACCCACAGCACCAATCCTACCTCCATCGCTAACCCCACTAAAAC +ACTCACCAAGACCTCAACCCCTGACCCCCATGCCTCAGGATACTCCTCAA +TAGCCATCGCTGTAGTATATCCAAAGACAACCATCATTCCCCCTAAATAA +ATTAAAAAAACTATTAAACCCATATAACCTCCCCCAAAATTCAGAATAAT +AACACACCCGACCACACCGCTAACAATCAATACTAAACCCCCATAAATAG +GAGAAGGCTTAGAAGAAAACCCCACAAACCCCATTACTAAACCCACACTC +AACAGAAACAAAGCATACATCATTATTCTCGCACGGACTACAACCACGAC +CAATGATATGAAAAACCATCGTTGTATTTCAACTACAAGAACACCAATGA +CCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTC +ATCGACCTCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTC +ACTCCTTGGCGCCTGCCTGATCCTCCAAATCACCACAGGACTATTCCTAG +CCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCAC +ATCACTCGAGACGTAAATTATGGCTGAATCATCCGCTACCTTCACGCCAA +TGGCGCCTCAATATTCTTTATCTGCCTCTTCCTACACATCGGGCGAGGCC +TATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATC +CTCCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTG +AGGCCAAATATCATTCTGAGGGGCCACAGTAATTACAAACTTACTATCCG +CCATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTAC +TCAGTAGACAGTCCCACCCTCACACGATTCTTTACCTTTCACTTCATCTT +GCCCTTCATTATTGCAGCCCTAGCAACACTCCACCTCCTATTCTTGCACG +AAACGGGATCAAACAACCCCCTAGGAATCACCTCCCATTCCGATAAAATC +ACCTTCCACCCTTACTACACAATCAAAGACGCCCTCGGCTTACTTCTCTT +CCTTCTCTCCTTAATGACATTAACACTATTCTCACCAGACCTCCTAGGCG +ACCCAGACAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATC +AAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCGATCCGTCCC +TAACAAACTAGGAGGCGTCCTTGCCCTATTACTATCCATCCTCATCCTAG +CAATAATCCCCATCCTCCATATATCCAAACAACAAAGCATAATATTTCGC +CCACTAAGCCAATCACTTTATTGACTCCTAGCCGCAGACCTCCTCATTCT +AACCTGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGAC +AAGTAGCATCCGTACTATACTTCACAACAATCCTAATCCTAATACCAACT +ATCTCCCTAATTGAAAACAAAATACTCAAATGGGCCTGTCCTTGTAGTAT +AAACTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCCAAGG +ACAAATCAGAGAAAAAGTCTTTAACTCCACCATTAGCACCCAAAGCTAAG +ATTCTAATTTAAACTATTCTCTGTTCTTTCATGGGGAAGCAGATTTGGGT +ACCACCCAAGTATTGACTCACCCATCAACAACCGCTATGTATTTCGTACA +TTACTGCCAGCCACCATGAATATTGTACGGTACCATAAATACTTGACCAC +CTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCTCCCCATGCTTA +CAAGcaagtacagcaatcaaccctcaactatcacacatcaactgcaactC +CAAAGCCACCCCTCACCCACTAGGATACCAACAAACCTACCCACCCTTAA +CAGTACATAGTACATAAAGCCATTTACCGTACATAGCACATTACAGTCAA +ATCCCTTCTCGTCCCCATGGATGACCCCCCTCAGATAGGGGTCCCTTGAC +CACCATCCTCCGTGAAATCAATATCCCGCACAAGAGTGCTACTCTCCTCG +CTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACAT +CTGGTTCCTACTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCCCT +TAAATAAGACATCACGATG
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/chrM_mouse.fa Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,327 @@ +>chrM +GTTAATGTAGCTTAATAACAAAGCAAAGCACTGAAAATGCTTAGATGGAT +AATTGTATCCCATAAACACAAAGGTTTGGTCCTGGCCTTATAATTAATTA +GAGGTAAAATTACACATGCAAACCTCCATAGACCGGTGTAAAATCCCTTA +AACATTTACTTAAAATTTAAGGAGAGGGTATCAAGCACATTAAAATAGCT +TAAGACACCTTGCCTAGCCACACCCCCACGGGACTCAGCAGTGATAAATA +TTAAGCAATAAACGAAAGTTTGACTAAGTTATACCTCTTAGGGTTGGTAA +ATTTCGTGCCAGCCACCGCGGTCATACGATTAACCCAAACTAATTATCTT +CGGCGTAAAACGTGTCAACTATAAATAAATAAATAGAATTAAAATCCAAC +TTATATGTGAAAATTCATTGTTAGGACCTAAACTCAATAACGAAAGTAAT +TCTAGTCATTTATAATACACGACAGCTAAGACCCAAACTGGGATTAGATA +CCCCACTATGCTTAGCCATAAACCTAAATAATTAAATTTAACAAAACTAT +TTGCCAGAGAACTACTAGCCATAGCTTAAAACTCAAAGGACTTGGCGGTA +CTTTATATCCATCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGCTCT +ACCTCACCATCTCTTGCTAATTCAGCCTATATACCGCCATCTTCAGCAAA +CCCTAAAAAGGTATTAAAGTAAGCAAAAGAATCAAACATAAAAACGTTAG +GTCAAGGTGTAGCCAATGAAATGGGAAGAAATGGGCTACATTTTCTTATA +AAAGAACATTACTATACCCTTTATGAAACTAAAGGACTAAGGAGGATTTA +GTAGTAAATTAAGAATAGAGAGCTTAATTGAATTGAGCAATGAAGTACGC +ACACACCGCCCGTCACCCTCCTCAAATTAAATTAAACTTAACATAATTAA +TTTCTAGACATCCGTTTATGAGAGGAGATAAGTCGTAACAAGGTAAGCAT +ACTGGAAAGTGTGCTTGGAATAATCATAGTGTAGCTTAATATTAAAGCAT +CTGGCCTACACCCAGAAGATTTCATGACCAATGAACACTCTGAACTAATC +CTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAAAC +ATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCT +ATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAA +CAAGCAAAGATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAA +CTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCAAACGAGCTAC +CTAAAAACAATTTTATGAATCAACTCGTCTATGTGGCAAAATAGTGAGAA +GATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTA +CCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAA +AATCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGG +AACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCA +TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT +AAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATT +GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG +AATTCCAATTCTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTT +AGTTAATCAGACTATAGGCAATAATCACACTATAAATAATCCACCTATAA +CTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGA +TAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTTACCAAAAACATCAC +CTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTT +aacggccgcggtatcctgaccgtgcaaaggtagcataatcacttgttcct +taattagggactagcatgaacggctaaacgagggtccaactgtctcttat +ctttaatcagtgaaattgacctttcagtgaagaggctgaaatataataat +aagacgagaagaccctatggagcttaaATTATATAACTTATCTATTTAAT +TTATTAAACCTAATGGCCCAAAAACTATAGTATAAGTTTGAAATTTCGGT +TGGGGTGACCTCGGAGAATAAAAAATCCTCCGAATGATTATAACCTAGAC +TTACAAGTCAAAGTAAAATCAACATATCTTATTGACCCAGATATATTTTG +ATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATCCTATTTAAGAG +TTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCC +CAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCT +ACGTGATCTGAGTTCAGACCGGAGCAATCCAGGTCGGTTTCTATCTATTT +ACGATTTCTCCCAGTACGAAAGGACAAGAGAAATAGAGCCACCTTACAAA +TAAGCGCTCTCAACTTAATTTATGAATAAAATCTAAATAAAATATATACG +TACACCCTCTAACCTAGAGAAGGTTAttagggtggcagagccaggaaatt +gcgtaagacttaaaaccttgttcccagaggttcaaatcctctccctaata +GTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCAT +AGCCTTCCTAACATTAGTAGAACGCAAAATCTTAGGGTACATACAACTAC +GAAAAGGCCCTAACATTGTTGGTCCATACGGCATTTTACAACCATTTGCA +GACGCCATAAAATTATTTATAAAAGAACCAATACGCCCTTTAACAACCTC +TATATCCTTATTTATTATTGCACCTACCCTATCACTCACACTAGCATTAA +GTCTATGAGTTCCCCTACCAATACCACACCCATTAATTAATTTAAACCTA +GGGATTTTATTTATTTTAGCAACATCTAGCCTATCAGTTTACTCCATTCT +ATGATCAGGATGAGCCTCAAACTCCAAATACTCACTATTCGGAGCTTTAC +GAGCCGTAGCCCAAACAATTTCATATGAAGTAACCATAGCTATTATCCTT +TTATCAGTTCTATTAATAAATGGATCCTACTCTCTACAAACACTTATTAC +AACCCAAGAACACATATGATTACTTCTGCCAGCCTGACCCATAGCCATAA +TATGATTTATCTCAACCCTAGCAGAAACAAACCGGGCCCCCTTCGACCTG +ACAGAAGGAGAATCAGAATTAGTATCAGGGTTTAACGTAGAATACGCAGC +CGGCCCATTCGCGTTATTCTTTATAGCAGAGTACACTAACATTATTCTAA +TAAACGCCCTAACAACTATTATCTTCCTAGGACCCCTATACTATATCAAT +TTACCAGAACTCTACTCAACTAACTTCATAATAGAAGCTCTACTACTATC +ATCAACATTCCTATGGATCCGAGCATCTTATCCACGCTTCCGTTACGATC +AACTTATACATCTTCTATGAAAAAACTTTCTACCCCTAACACTAGCATTA +TGTATGTGACATATTTCTTTACCAATTTTTACAGCGGGAGTACCACCATA +CATATAGAAATATGTCTGATAAAAGAATTACTTTGATAGAGTAAATTATA +GAGGTTCAAGCCCTCTTATTTctaggacaataggaattgaacctacactt +aagaattcaaaattctccgtgctacctaaacaccttatcctaATAGTAAG +GTCAGCTAATTAAGCTATCGGGCCCATACCCCGAAAACGTTGGTTTAAAT +CCTTCCCGTACTAATAAATCCTATCACCCTTGCCATCATCTACTTCACAA +TCTTCTTAGGTCCTGTAATCACAATATCCAGCACCAACCTAATACTAATA +TGAGTAGGCCTGGAATTCAGCCTACTAGCAATTATCCCCATACTAATCAA +CAAAAAAAACCCACGATCAACTGAAGCAGCAACAAAATACTTCGTCACAC +AAGCAACAGCCTCAATAATTATCCTCCTGGCCATCGTACTCAACTATAAA +CAACTAGGAACATGAATATTTCAACAACAAACAAACGGTCTTATCCTTAA +CATAACATTAATAGCCCTATCCATAAAACTAGGCCTCGCCCCATTCCACT +TCTGATTACCAGAAGTAACTCAAGGGATCCCACTGCACATAGGACTTATT +CTTCTTACATGACAAAAAATTGCTCCCCTATCAATTTTAATTCAAATTTA +CCCGCTACTCAACTCTACTATCATTTTAATACTAGCAATTACTTCTATTT +TCATAGGGGCATGAGGAGGACTTAACCAAACACAAATACGAAAAATTATA +GCCTATTCATCAATTGCCCACATAGGATGAATATTAGCAATTCTTCCTTA +CAACCCATCCCTCACTCTACTCAACCTCATAATCTATATTATTCTTACAG +CCCCTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCA +ATCTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACT +GATATTACTATCCCTAGGAGGCCTTCCACCACTAACAGGATTCTTACCAA +AATGAATTATCATCACAGAACTTATAAAAAACAACTGTCTAATTATAGCA +ACACTCATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCGCCT +AATTTATTCCACTTCACTAACAATATTTCCAACCAACAATAACTCAAAAA +TAATAACTCACCAAACAAAAACTAAACCCAACCTAATATTTTCCACCCTA +GCTATCATAAGCACAATAACCCTACCCCTAGCCCCCCAACTAATTACCTA +GAAGTTTAGGATATACTAGTCCGCGAGCCTTCAAAGCCCTAAGAAAACAC +ACAAGTTTAACTTCTGATAAGGACTGTAAGACTTCATCCTACATCTATTG +AATGCAAATCAATTGCTTTAATTAAGCTAAGACCTCAACTAGATTGGCAG +GAATTAAACCTACGAAAATTTAGTTAACAGCTAAATACCCTATTACTGGC +TTCAATCTACTTCTACCGCCGAAAAAAAAAAATGGCGGTAGAAGTCTTAG +TAGAGATTTCTCTACACCTTCGAATTTGCAATTCGACATGAATATCACCT +TAAGACCTCTGGTAAAAAGAGGATTTAAACCTCTGTGTTTAGATTTACAG +TCTAATGCTTACTCAGCCATTTTACCTATGTTCATTAATCGTTGATTATT +CTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTATTCGGAGCCT +GAGCGGGAATAGTGGGTACTGCACTAAGTATTTTAATTCGAGCAGAATTA +GGTCAACCAGGTGCACTTTTAGGAGATGACCAAATTTACAATGTTATCGT +AACTGCCCATGCTTTTGTTATAATTTTCTTCATAGTAATACCAATAATAA +TTGGAGGCTTTGGAAACTGACTTGTCCCACTAATAATCGGAGCCCCAGAT +ATAGCATTCCCACGAATAAATAATATAAGTTTTTGACTCCTACCACCATC +ATTTCTCCTTCTCCTAGCATCATCAATAGTAGAAGCAGGAGCAGGAACAG +GATGAACAGTCTACCCACCTCTAGCCGGAAATCTAGCCCATGCAGGAGCA +TCAGTAGACCTAACAATTTTCTCCCTTCATTTAGCTGGAGTGTCATCTAT +TTTAGGTGCAATTAATTTTATTACCACTATTATCAACATGAAACCCCCAG +CCATAACACAGTATCAAACTCCACTATTTGTCTGATCCGTACTTATTACA +GCCGTACTGCTCCTATTATCACTACCAGTGCTAGCCGCAGGCATTACTAT +ACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCCGCTGGAG +GAGGGGACCCAATTCTCTACCAGCATCTGTTCTGATTCTTTGGGCACCCA +GAAGTTTATATTCTTATCCTCCCAGGATTTGGAATTATTTCACATGTAGT +TACTTACTACTCCGGAAAAAAAGAACCTTTCGGCTATATAGGAATAGTAT +GAGCAATAATGTCTATTGGCTTTCTAGGCTTTATTGTATGAGCCCACCAC +ATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAGC +CACTATAATTATCGCAATTCCTACCGGTGTCAAAGTATTTAGCTGACTTG +CAACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCC +TTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCTAACCGGAATTGTTTT +ATCCAACTCATCCCTTGACATCGTGCTTCACGATACATACTATGTAGTAG +CCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTGCTATCATAGCA +GGATTTGTTCACTGATTCCCATTATTTTCAGGCTTCACCCTAGATGACAC +ATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAACATAACAT +TCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCA +GACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATC +ATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGG +CCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAAT +TTAGAATGACTTCATGGCTGCCCTCCACCATATCACACATTCGAGGAACC +AACCTATGTAAAAGTAAAAtaagaaaggaaggaatcgaaccccctaaaat +tggtttcaagccaatctcatatcctatatgtctttctcAATAAGATATTA +GTAAAATCAATTACATAACTTTGTCAAAGTTAAATTATAGATCAATAATC +TATATATCTTATATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCAC +ATCCCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAA +TTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCTAATATTA +ACAACAAAACTAACACATACAAGCACAATAGATGCACAAGAAGTTGAAAC +CATTTGAACTATTCTACCAGCTGTAATCCTTATCATAATTGCTCTCCCCT +CTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTATTAACC +GTTAAAACCATAGGGCACCAATGATACTGAAGCTACGAATATACTGACTA +TGAAGACCTATGCTTTGATTCATATATAATCCCAACAAACGACCTAAAAC +CTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTTCTGCCAATA +GAACTTCCAATCCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATG +AGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAA +ATCAAGCAACAGTAACATCAAACCGACCAGGGTTATTCTATGGCCAATGC +TCTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAAT +GGTTCCACTAAAATATTTCGAAAACTGATCTGCTTCAATAATTTAATTTC +ACTATGAAGCTAAGAGCGTTAACCTTTTAAGTTAAAGTTAGAGACCTTAA +AATCTCCATAGTGATATGCCACAACTAGATACATCAACATGATTTATCAC +AATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCT +CATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATA +AAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCA +TTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTAT +ATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTC +TCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATG +CTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCT +AATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACAT +TTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTA +TGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACT +TGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTA +TTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTC +CGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGG +AGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTA +CATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTA +ATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAA +TACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCC +ATGACCATTAACTGGAGCCTTTTCAGCCCTCCTTCTAACATCAGGTCTAG +TAATATGATTTCACTATAATTCAATTACACTATTAACCCTTGGCCTACTC +ACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGG +AACCTACCAAGGCCACCACACTCCTATTGTACAAAAAGGACTACGATATG +GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC +TGAGCGTTCTATCATTCTAGCCTCGTACCAACACATGATCTAGGAGGCTG +CTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTAC +TTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCAT +CATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAAT +TACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACT +TTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTCTACATTCTTC +ATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCT +TATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATC +ACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTC +TGACTTTTCCTATACGTCTCCATTTATTGATGAGGATCTTACTCCCTTAG +TATAATTAATATAACTGACTTCCAATTAGTAGATTCTGAATAAACCCAGA +AGAGAGTAATTAACCTGTACACTGTTATCTTCATTAATATTTTATTATCC +CTAACGCTAATTCTAGTTGCATTCTGACTCCCCCAAATAAATCTGTACTC +AGAAAAAGCAAATCCATATGAATGCGGATTCGACCCTACAAGCTCTGCAC +GTCTACCATTCTCAATAAAATTTTTCTTGGTAGCAATTACATTTCTATTA +TTTGACCTAGAAATTGCTCTTCTACTTCCACTACCATGAGCAATTCAAAC +AATTAAAACCTCTACTATAATAATTATAGCCTTTATTCTAGTCACAATTC +TATCTCTAGGCCTAGCATATGAATGAACACAAAAAGGATTAGAATGAACA +GAGTAAATGGTAATTAGTTTAAAAAAAATTAATGATTTCGACTCATTAGA +TTATGATGATGTTCATAATTACCAATATGCCATCTACCTTCTTCAACCTC +ACCATAGCCTTCTCACTATCACTTCTAGGGACACTTATATTTCGCTCTCA +CCTAATATCCACATTACTATGCCTGGAAGGCATAGTATTATCCTTATTTA +TTATAACTTCAGTAACTTCCCTAAACTCCAACTCCATAAGCTCCATACCA +ATCCCCATCACCATCTTAGTTTTCGCAGCCTGCGAAGCAGCTGTAGGACT +AGCCCTACTAGTAAAAGTTTCAAACACGTACGGAACAGATTACGTCCAAA +ATCTCAACCTACTACAATGCTAAAAATTATTCTTCCCTCACTAATGCTAC +TACCACTAACCTGACTATCAAGCCCTAAAAAAACCTGAACAAACGTAACC +TCATATAGTTTTCTAATTAGTTTAACCAGCCTAACACTTCTATGACAAAC +CGACGAAAATTATAAAAACTTTTCAAATATATTCTCCTCAGACCCCCTAT +CCACACCATTAATTATTTTAACAGCCTGATTACTGCCACTAATATTAATA +GCTAGCCAAAACCACCTAAAAAAAGATAATAACGTACTACAAAAACTCTA +CATCTCAATACTAATCAGCTTACAAATTCTCCTAATCATAACCTTTTCAG +CAACTGAACtaattatattttatattttatttGAAGCAACCTTAATCCCA +ACACTTATTATTATTACCCGATGAGGGAACCAAACTGAACGCCTAAACGC +AGGGATTTATTTCCTATTTTATACCCTAATCGGTTCTATTCCACTGCTAA +TTGCCCTCATCTTAATCCAAAACCATGTAGGAACCCTAAACCTCATAATT +TTATCATTCACAACACACACCTTAGACGCTTCATGATCTAACAACTTACT +ATGGTTGGCATGCATAATAGCATTTCTTATTAAAATACCATTATATGGAG +TTCACCTATGACTACCAAAAGCCCATGTTGAAGCTCCAATTGCTGGGTCA +ATAATTCTAGCAGCTATTCTTCTAAAATTAGGTAGTTACGGAATAATTCG +CATCTCCATTATTCTAGACCCACTAACAAAATATATAGCATACCCCTTCA +TCCTTCTCTCCCTATGAGGAATAATTATAACTAGCTCAATCTGCTTACGC +CAAACAGATTTAAAATCACTAATCGCCTACTCCTCAGTTAGCCACATAGC +ACTTGTTATTGCATCAATCATAATCCAAACTCCATGAAGCTTCATAGGAG +CAACAATACTAATAATCGCACATGGCCTCACATCATCACTCCTATTCTGC +CTAGCAAACTCCAACTACGAACGGATCCACAGCCGTACTATAATCATGGC +CCGAGGACTTCAAATGGTCTTCCCACTTATAGCCACATGATGACTGATAG +CAAGTCTAGCTAATCTAGCTCTACCCCCTTCAATCAATCTAATAGGAGAA +TTATTCATTACCATATCATTATTTTCTTGATCAAACTTTACCATTATTCT +TATAGGAATTAACATTATTATTACAGGTATATACTCAATATACATAATTA +TTACCACCCAACGCGGCAAACTAACCAACCATATAATTAACCTCCAACCC +TCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTAT +TCTTCTAACTACCAGTCCAAAACTAATTACAGGCCTGACAATATGTGAAT +ATAGTTTACAAAAAACATTAGACTGTGAATCTGACAACAGGAAATAAACC +TCCTTATTCACCAAGAAAGATTGCAAGAACTGCTAATTCATGCTTCCATG +TTTAAAAACATGGCTTTCTTACTTTTATAGGATAATAGTAATCCATTGGT +CTTAGGAACCAAAAACCTTGGTGCAAATCCAAATAAAAGTAATCAATATT +TTCACAACCTCAATCTTATTAATCTTCATTCTTCTACTATCCCCAATCCT +AATTTCAATATCAAACCTAATTAAACACATCAACTTCCCACTGTACACCA +CCACATCAATCAAATTCTCCTTCATTATTAGCCTCTTACCCCTATTAATA +TTTTTCCACAATAATATAGAATATATAATTACAACCTGGCACTGAGTCAC +CATAAATTCAATAGAACTTAAAATAAGCTTCAAAACTGACTTTTTCTCTA +TCCTGTTTACATCTGTAGCCCTTTTTGTCACATGATCAATTATACAATTC +TCTTCATGATATATACACTCAGACCCAAACATCAATCGATTCATTAAATA +TCTTACACTATTCCTGATTACCATGCTTATCCTCACCTCAGCCAACAACA +TATTTCAACTTTTCATTGGCTGAGAAGGGGTGGGAATTATATCTTTCCTA +CTAATTGGATGATGGTACGGACGAACAGACGCAAATACTGCAGCCCTACA +AGCAATCCTCTATAACCGCATCGGAGACATCGGATTCATTTTAGCTATAG +TTTGATTTTCCCTAAACATAAACTCATGAGAACTTCAACAGATTATATTC +TCCAACAACAACGACAATCTAATTCCACTTATAGGCCTATTAATCGCAGC +TACAGGAAAATCAGCACAATTTGGCCTCCACCCATGACTACCATCAGCAA +TAGAAGGCCCTACACCAGTTTCAGCACTACTACACTCAAGTACAATAGTA +GTTGCAGGAATTTTCCTACTGGTCCGATTCCACCCCCTCACGACTAATAA +TAACTTTATTTTAACAACTATACTTTGCCTCGGAGCCCTAACCACATTAT +TTACAGCTATTTGTGCTCTCACCCAAAACGACATCAAAAAAATCATTGCC +TTCTCTACATCAAGCCAACTAGGCCTGATAATAGTGACGCTAGGAATAAA +CCAACCACACCTAGCATTCCTACACATCTGTACCCACGCATTCTTCAAAG +CTATACTCTTTATATGCTCTGGCTCAATCATTCATAGCCTGGCAGACGAA +CAAGACATCCGAAAAATAGGAAACATCACAAAAATCATACCATTCACATC +ATCATGCCTAGTAATCGGAAGCCTCGCCCTCACAGGAATACCATTCCTAA +CAGGGTTCTACTCAAAAGACCTAATTATTGAAGCAATTAATACCTGCAAC +ACCAACGCCTGAGCCCTACTAATTACACTAATCGCCACTTCTATAACAGC +TATGTACAGCATACGAATCATTTACTTCGTAACAATAACAAAACCGCGTT +TTCCCCCCCTAATCTCCATTAACGAAAATGACCCAGACCTCATAAACCCA +ATCAAACGCCTAGCATTCGGAAGCATCTTTGCAGGATTTGTCATCTCATA +TAATATTCCACCAACCAGCATTCCAGTCCTCACAATACCATGATTTTTAA +AAACCACAGCCCTAATTATTTCAGTATTAGGATTCCTAATCGCACTAGAA +CTAAACAACCTAACCATAAAACTATCAATAAATAAAGCAAATCCATATTC +ATCCTTCTCAACTTTACTGGGGTTTTTCCCATCTATTATTCACCGCATTA +CACCCATAAAATCTCTCAACCTAAGCCTAAAAACATCCCTAACTCTCCTA +GACTTGATCTGGTTAGAAAAAACCATCCCAAAATCCACCTCAACTCTTCA +CACAAACATAACCACTTTAACAACCAACCAAAAAGGCTTAATTAAATTGT +ACTTTATATCATTCCTAATTAACATCATCTTAATTATTATCTTATACTCA +ATTAATCTCGAGTAATCTCGATAATAATAAAAATACCCGCAAACAAAGAT +CACCCAGCTACTACCATCATTCAAGTAGCACAACTATATATTGCCGCTAC +CCCAATCCCTCCTTCCAACATAACTCCAACATCATCAACCTCATACATCA +ACCAATCTCCCAAACCATCAAGATTAATTACTCCAACTTCATCATAATAA +TTAAGCACACAAATTAAAAAAACCTCTATAATCACCCCCAATACTAAAAA +ACCCAAAATTAATCAGTTAGATCCCCAAGTCTCTGGATATTCCTCAGTAG +CTATAGCAGTCGTATATCCAAACACAACCAACATCCCCCCTAAATAAATT +AAAAAAACTATTAAACCTAAAAACGATCCACCAAACCCTAAAACCATTAA +ACAACCAACAAACCCACTAACAATTAAACCTAAACCTCCATAAATAGGTG +AAGGCTTTAATGCTAACCCAAGACAACCAACCAAAAATAATGAACTTAAA +ACAAAAATATAATTATTCATTATTTCTACACAGCATTCAACTGCGACCAA +TGACATGAAAAATCATCGTTGTAATTCAACTACAGAAACACCTAATGACA +AACATACGAAAAACACACCCATTATTTAAAATTATTAACCACTCATTCAT +TGACCTACCTGCCCCATCCAACATTTCATCATGATGAAACTTTGGGTCCC +TTCTAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCC +ATACACTACACATCAGATACAATAACAGCCTTTTCATCAGTAACACACAT +TTGTCGAGACGTAAATTACGGGTGACTAATCCGATATATACACGCAAACG +GAGCCTCAATATTTTTTATTTGCTTATTCCTTCATGTCGGACGAGGCTTA +TATTATGGATCATATACATTTATAGAAACCTGAAACATTGGAGTACTTCT +ACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCCATGAG +GACAAATATCATTCTGAGGTGCCACAGTTATTACAAACCTCCTATCAGCC +ATCCCATATATTGGAACAACCCTAGTCGAATGAATTTGAGGGGGCTTCTC +AGTAGACAAAGCCACCTTGACCCGATTCTTCGCTTTCCACTTCATCTTAC +CATTTATTATCGCGGCCCTAGCAATCGTTCACCTCCTCTTCCTCCACGAA +ACAGGATCAAACAACCCAACAGGATTAAACTCAGATGCAGATAAAATTCC +ATTTCACCCCTACTATACAATCAAAGATATCCTAGGTATCCTAATCATAT +TCTTAATTCTCATAACCCTAGTATTATTTTTCCCAGACATACTAGGAGAC +CCAGACAACTACATACCAGCTAATCCACTAAACACCCCACCCCATATTAA +ACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAATCCCCA +ATAAACTAGGAGGTGTCCTAGCCTTAATCTTATCTATCCTAATTTTAGCC +CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC +AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA +CCTGAATTGGGGGCCAACCAGTAGAACACCCATTTATTATCATTGGCCAA +CTAGCCTCCATCTCATACTTCTCAATCATCTTAATTCTTATACCAATCTC +AGGAATTATCGAAGACAAAATACTAAAATTATATCCATGTCTTGATAGTA +TAAACATTACTCTGGTCTTGTAAACCTGAAATGAAGATCTTCTCTTCTCA +AGACATCAAGAAGAAGGAGCTACTCCCCACCACCAGCACCCAAAGCTGGT +ATTCTAATTAAACTACTTCTTGAGTACATAAATTTACATAGTACAACAGT +ACATTTATGTATATCGTACATTAAACTATTTTCCCCAAGCATATAAGCTA +GTACATTAAATCAATGGTTCAGGTCATAAAATAATCATCAACATAAATCA +ATATATATACCATGAATATTATCTTAAACACATTAAACTAATGTTATAAG +GACATATCTGTGTTATCTGACATACACCATACAGTCATAAACTCTTCTCT +TCCATATGACTATCCCCTTCCCCATTTGGTCTATTAATCTACCATCCTCC +GTGAAACCAACAACCCGCCCACCAATGCCCCTCTTCTCGCTCCGGGCCCA +TTAAACTTGGGGGTAGCTAAACTGAAACTTTATCAGACATCTGGTTCTTA +CTTCAGGGCCATCAAATGCGTTATCGCCCATACGTTCCCCTTAAATAAGA +CATCTCGATGGTATCGGGTCTAATCAGCCCATGACCAACATAACTGTGGT +GTCATGCATTTGGTATCTTTTTATTTTGGCCTACTTTCATCAACATAGCC +GTCAAGGCATGAAAGGACAGCACACAGTCTAGACGCACCTACGGTGAAGA +ATCATTAGTCCGCAAAACCCAATCACCTAAGGCTAATTATTCATGCTTGT +TAGACATAAATGCTACTCAATACCAAATTTTAACTCTCCAAACCCCCCAC +CCCCTCCTCTTAATGCCAAACCCCAAAAACACTAAGAACTTGAAAGACAT +ATAATATTAACTATCAAACCCTATGTCCTGATCAATTCTAGTAGTTCCCA +AAATATGACTTATATTTTAGTACTTGTAAAAATTTTACAAAATCATGTTC +CGTGAACCAAAACTCTAATCATACTCTATTACGCAATAAACATTAACAA
--- a/test-data/lastz_census.txt Tue Oct 13 12:23:37 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,5386 +0,0 @@ -phiX 1 255 -phiX 2 255 -phiX 3 255 -phiX 4 255 -phiX 5 255 -phiX 6 255 -phiX 7 255 -phiX 8 255 -phiX 9 255 -phiX 10 255 -phiX 11 255 -phiX 12 255 -phiX 13 255 -phiX 14 255 -phiX 15 255 -phiX 16 255 -phiX 17 255 -phiX 18 255 -phiX 19 255 -phiX 20 255 -phiX 21 255 -phiX 22 255 -phiX 23 255 -phiX 24 255 -phiX 25 255 -phiX 26 255 -phiX 27 255 -phiX 28 255 -phiX 29 255 -phiX 30 255 -phiX 31 255 -phiX 32 255 -phiX 33 255 -phiX 34 255 -phiX 35 255 -phiX 36 255 -phiX 37 255 -phiX 38 255 -phiX 39 255 -phiX 40 255 -phiX 41 255 -phiX 42 255 -phiX 43 255 -phiX 44 255 -phiX 45 255 -phiX 46 255 -phiX 47 255 -phiX 48 255 -phiX 49 255 -phiX 50 255 -phiX 51 255 -phiX 52 255 -phiX 53 255 -phiX 54 255 -phiX 55 255 -phiX 56 255 -phiX 57 255 -phiX 58 255 -phiX 59 255 -phiX 60 255 -phiX 61 255 -phiX 62 255 -phiX 63 255 -phiX 64 255 -phiX 65 255 -phiX 66 255 -phiX 67 255 -phiX 68 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-phiX 5065 255 -phiX 5066 255 -phiX 5067 255 -phiX 5068 255 -phiX 5069 255 -phiX 5070 255 -phiX 5071 255 -phiX 5072 255 -phiX 5073 255 -phiX 5074 255 -phiX 5075 255 -phiX 5076 255 -phiX 5077 255 -phiX 5078 255 -phiX 5079 255 -phiX 5080 255 -phiX 5081 255 -phiX 5082 255 -phiX 5083 255 -phiX 5084 255 -phiX 5085 255 -phiX 5086 255 -phiX 5087 255 -phiX 5088 255 -phiX 5089 255 -phiX 5090 255 -phiX 5091 255 -phiX 5092 255 -phiX 5093 255 -phiX 5094 255 -phiX 5095 255 -phiX 5096 255 -phiX 5097 255 -phiX 5098 255 -phiX 5099 255 -phiX 5100 255 -phiX 5101 255 -phiX 5102 255 -phiX 5103 255 -phiX 5104 255 -phiX 5105 255 -phiX 5106 255 -phiX 5107 255 -phiX 5108 255 -phiX 5109 255 -phiX 5110 255 -phiX 5111 255 -phiX 5112 255 -phiX 5113 255 -phiX 5114 255 -phiX 5115 255 -phiX 5116 255 -phiX 5117 255 -phiX 5118 255 -phiX 5119 255 -phiX 5120 255 -phiX 5121 255 -phiX 5122 255 -phiX 5123 255 -phiX 5124 255 -phiX 5125 255 -phiX 5126 255 -phiX 5127 255 -phiX 5128 255 -phiX 5129 255 -phiX 5130 255 -phiX 5131 255 -phiX 5132 255 -phiX 5133 255 -phiX 5134 255 -phiX 5135 255 -phiX 5136 255 -phiX 5137 255 -phiX 5138 255 -phiX 5139 255 -phiX 5140 255 -phiX 5141 255 -phiX 5142 255 -phiX 5143 255 -phiX 5144 255 -phiX 5145 255 -phiX 5146 255 -phiX 5147 255 -phiX 5148 255 -phiX 5149 255 -phiX 5150 255 -phiX 5151 255 -phiX 5152 255 -phiX 5153 255 -phiX 5154 255 -phiX 5155 255 -phiX 5156 255 -phiX 5157 255 -phiX 5158 255 -phiX 5159 255 -phiX 5160 255 -phiX 5161 255 -phiX 5162 255 -phiX 5163 255 -phiX 5164 255 -phiX 5165 255 -phiX 5166 255 -phiX 5167 255 -phiX 5168 255 -phiX 5169 255 -phiX 5170 255 -phiX 5171 255 -phiX 5172 255 -phiX 5173 255 -phiX 5174 255 -phiX 5175 255 -phiX 5176 255 -phiX 5177 255 -phiX 5178 255 -phiX 5179 255 -phiX 5180 255 -phiX 5181 255 -phiX 5182 255 -phiX 5183 255 -phiX 5184 255 -phiX 5185 255 -phiX 5186 255 -phiX 5187 255 -phiX 5188 255 -phiX 5189 255 -phiX 5190 255 -phiX 5191 255 -phiX 5192 255 -phiX 5193 255 -phiX 5194 255 -phiX 5195 255 -phiX 5196 255 -phiX 5197 255 -phiX 5198 255 -phiX 5199 255 -phiX 5200 255 -phiX 5201 255 -phiX 5202 255 -phiX 5203 255 -phiX 5204 255 -phiX 5205 255 -phiX 5206 255 -phiX 5207 255 -phiX 5208 255 -phiX 5209 255 -phiX 5210 255 -phiX 5211 255 -phiX 5212 255 -phiX 5213 255 -phiX 5214 255 -phiX 5215 255 -phiX 5216 255 -phiX 5217 255 -phiX 5218 255 -phiX 5219 255 -phiX 5220 255 -phiX 5221 255 -phiX 5222 255 -phiX 5223 255 -phiX 5224 255 -phiX 5225 255 -phiX 5226 255 -phiX 5227 255 -phiX 5228 255 -phiX 5229 255 -phiX 5230 255 -phiX 5231 255 -phiX 5232 255 -phiX 5233 255 -phiX 5234 255 -phiX 5235 255 -phiX 5236 255 -phiX 5237 255 -phiX 5238 255 -phiX 5239 255 -phiX 5240 255 -phiX 5241 255 -phiX 5242 255 -phiX 5243 255 -phiX 5244 255 -phiX 5245 255 -phiX 5246 255 -phiX 5247 255 -phiX 5248 255 -phiX 5249 255 -phiX 5250 255 -phiX 5251 255 -phiX 5252 255 -phiX 5253 255 -phiX 5254 255 -phiX 5255 255 -phiX 5256 255 -phiX 5257 255 -phiX 5258 255 -phiX 5259 255 -phiX 5260 255 -phiX 5261 255 -phiX 5262 255 -phiX 5263 255 -phiX 5264 255 -phiX 5265 255 -phiX 5266 255 -phiX 5267 255 -phiX 5268 255 -phiX 5269 255 -phiX 5270 255 -phiX 5271 255 -phiX 5272 255 -phiX 5273 255 -phiX 5274 255 -phiX 5275 255 -phiX 5276 255 -phiX 5277 255 -phiX 5278 255 -phiX 5279 255 -phiX 5280 255 -phiX 5281 255 -phiX 5282 255 -phiX 5283 255 -phiX 5284 255 -phiX 5285 255 -phiX 5286 255 -phiX 5287 255 -phiX 5288 255 -phiX 5289 255 -phiX 5290 255 -phiX 5291 255 -phiX 5292 255 -phiX 5293 255 -phiX 5294 255 -phiX 5295 255 -phiX 5296 255 -phiX 5297 255 -phiX 5298 255 -phiX 5299 255 -phiX 5300 255 -phiX 5301 255 -phiX 5302 255 -phiX 5303 255 -phiX 5304 255 -phiX 5305 255 -phiX 5306 255 -phiX 5307 255 -phiX 5308 255 -phiX 5309 255 -phiX 5310 255 -phiX 5311 255 -phiX 5312 255 -phiX 5313 255 -phiX 5314 255 -phiX 5315 255 -phiX 5316 255 -phiX 5317 255 -phiX 5318 255 -phiX 5319 255 -phiX 5320 255 -phiX 5321 255 -phiX 5322 255 -phiX 5323 255 -phiX 5324 255 -phiX 5325 255 -phiX 5326 255 -phiX 5327 255 -phiX 5328 255 -phiX 5329 255 -phiX 5330 255 -phiX 5331 255 -phiX 5332 255 -phiX 5333 255 -phiX 5334 255 -phiX 5335 255 -phiX 5336 255 -phiX 5337 255 -phiX 5338 255 -phiX 5339 255 -phiX 5340 255 -phiX 5341 255 -phiX 5342 255 -phiX 5343 255 -phiX 5344 255 -phiX 5345 255 -phiX 5346 255 -phiX 5347 255 -phiX 5348 255 -phiX 5349 255 -phiX 5350 255 -phiX 5351 255 -phiX 5352 255 -phiX 5353 255 -phiX 5354 255 -phiX 5355 255 -phiX 5356 255 -phiX 5357 255 -phiX 5358 255 -phiX 5359 255 -phiX 5360 255 -phiX 5361 255 -phiX 5362 255 -phiX 5363 255 -phiX 5364 255 -phiX 5365 255 -phiX 5366 255 -phiX 5367 255 -phiX 5368 255 -phiX 5369 255 -phiX 5370 255 -phiX 5371 255 -phiX 5372 255 -phiX 5373 255 -phiX 5374 255 -phiX 5375 255 -phiX 5376 255 -phiX 5377 255 -phiX 5378 255 -phiX 5379 255 -phiX 5380 255 -phiX 5381 255 -phiX 5382 255 -phiX 5383 255 -phiX 5384 255 -phiX 5385 255 -phiX 5386 255
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/lastz_d_ctrl_file.txt Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,23 @@ +# base the inference on alignments in the middle half by identity +min_identity = 25.0% # 25th percentile +max_identity = 75.0% # 75th percentile + +# scale scores so max substitution score will be 100, and only use +# alignments scoring at least as well as 20 ideal matches +inference_scale = 100 # max substitution score +hsp_threshold = 20*inference_scale +gapped_threshold = hsp_threshold + +# allow substitution score inference to iterate at most 20 times; +# don't perform gap penalty inference -- instead hardwire gap penalties +# relative to max substitution +max_sub_iterations = 20 +max_gap_iterations = 0 +gap_open_penalty = 4*inference_scale +gap_extend_penalty = 0.3*inference_scale + +# use all seedword positions (don't sample) +step = 1 + +# adjust for entropy when qualifying HSPs +entropy = on
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/lastz_d_test1.out Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,12 @@ +# (a LASTZ scoring set, created by "LASTZ --infer") + +bad_score = X:-1764 # used for sub[X][*] and sub[*][X] +fill_score = -176 # used when sub[*][*] not otherwise defined +gap_open_penalty = 573 +gap_extend_penalty = 43 + + A C G T +A 73 -83 -52 -100 +C -83 100 -176 -52 +G -52 -176 100 -83 +T -100 -52 -83 73
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/lastz_d_test2.out Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,12 @@ +# (a LASTZ scoring set, created by "LASTZ --infer") + +bad_score = X:-1781 # used for sub[X][*] and sub[*][X] +fill_score = -178 # used when sub[*][*] not otherwise defined +gap_open_penalty = 400 +gap_extend_penalty = 30 + + A C G T +A 72 -79 -49 -97 +C -79 100 -178 -49 +G -49 -178 100 -79 +T -97 -49 -79 72
--- a/test-data/lastz_diffs.txt Tue Oct 13 12:23:37 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,19 +0,0 @@ -phiX 2490 2491 + 5386 HWI-EAS91_1_306UPAAXX:6:1:486:822 10 11 - 36 C A ACCTGTTTTACAGACACCTAAAGCTACATCGTCAAC ACCTGTTTTAAAGACACCTAAAGCTACATCGTCAAC -phiX 2173 2174 + 5386 HWI-EAS91_1_306UPAAXX:6:1:259:1389 26 27 + 36 G T GCGTACTTATTCGCCACCATGATTATGACCAGTGTT GCGTACTTATTCGCCACCATGATTATTACCAGTGTT -phiX 578 579 + 5386 HWI-EAS91_1_306UPAAXX:6:1:317:1411 8 9 + 36 G T GTACGCTGGACTTTGTGGGATACCCTCGCTTTCCT GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCT -phiX 2992 2993 + 5386 HWI-EAS91_1_306UPAAXX:6:1:458:1629 23 24 + 36 G T GCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGT GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT -phiX 4783 4784 + 5386 HWI-EAS91_1_306UPAAXX:6:1:646:59 18 19 - 36 C T CATTTCCATGCGGTGCACTTTATGCGGACACTTCC CATTTCCATGCGGTGCATTTTATGCGGACACTTCC -phiX 1649 1650 + 5386 HWI-EAS91_1_306UPAAXX:6:1:295:694 24 25 + 36 A G GTGATTACTTCATGCAGCGTTACCATGATGTTATTT GTGATTACTTCATGCAGCGTTACCGTGATGTTATTT -phiX 1995 1996 + 5386 HWI-EAS91_1_306UPAAXX:6:1:590:331 14 15 + 36 G T GAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGAT GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT -phiX 3261 3262 + 5386 HWI-EAS91_1_306UPAAXX:6:1:221:1238 17 18 - 36 C A AAGTCTGCCGCTGATAAAGGAAAGGATAC AAGTCTGCCGATGATAAAGGAAAGGATAC -phiX 4109 4110 + 5386 HWI-EAS91_1_306UPAAXX:6:1:478:252 12 13 - 36 C A ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC ATGCTTGGCTTCAATAAGCAGATGGATAACCGCATC -phiX 2972 2973 + 5386 HWI-EAS91_1_306UPAAXX:6:1:836:1204 17 18 + 36 G T TTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAAT TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT -phiX 2234 2235 + 5386 HWI-EAS91_1_306UPAAXX:6:1:172:1412 13 14 - 36 C A AAATTTAATGTGACCGTTTATCGCAATCTGCCGACC AAATTTAATGTGAACGTTTATCGCAATCTGCCGACC -phiX 2902 2903 + 5386 HWI-EAS91_1_306UPAAXX:6:1:540:1415 18 19 + 36 G T TTATTAAAGAGATTATTTGTCTCCAGCCACTTA TTATTAAAGAGATTATTTTTCTCCAGCCACTTA -phiX 2837 2838 + 5386 HWI-EAS91_1_306UPAAXX:6:1:253:1162 14 15 - 36 T A ATGGTTTGGTCTAACTTTACCGCTACTAAATGC ATGGTTTGGTCAAACTTTACCGCTACTAAATGC -phiX 4662 4663 + 5386 HWI-EAS91_1_306UPAAXX:6:1:828:754 26 27 + 36 G T TTTGCGTGACTATTTTCGTGATATTGGTCGTATGGT TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT -phiX 4783 4784 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1566:499 9 10 + 36 C T TGCGGTGCACTTTATGCGGACACTTCCTACAGGTAG TGCGGTGCATTTTATGCGGACACTTCCTACAGGTAG -phiX 2857 2858 + 5386 HWI-EAS91_1_306UPAAXX:6:1:578:971 15 16 + 36 G T CGCTACTAAATGCCGCGGATTGGTTTCG CGCTACTAAATTCCGCGGATTGGTTTCG -phiX 3112 3113 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1721:1800 27 28 - 36 T C TCAAGGCTCTAATGTTCCTAACCC TCAAGGCTCTAATGTCCCTAACCC -phiX 4825 4826 + 5386 HWI-EAS91_1_306UPAAXX:6:1:815:1640 9 10 - 36 T G CCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCA CCCTAATTTGGGTCGTCGGGTACGCAATCGCCGCCA -phiX 4517 4518 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1485:741 19 20 - 36 G A GAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGAT GAAATCGCGTAGAGGCTTTACTATTCAGCGTTTGAT
--- a/test-data/lastz_diffs_ref_name.txt Tue Oct 13 12:23:37 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,19 +0,0 @@ -Ref 2490 2491 + 5386 HWI-EAS91_1_306UPAAXX:6:1:486:822 10 11 - 36 C A ACCTGTTTTACAGACACCTAAAGCTACATCGTCAAC ACCTGTTTTAAAGACACCTAAAGCTACATCGTCAAC -Ref 2173 2174 + 5386 HWI-EAS91_1_306UPAAXX:6:1:259:1389 26 27 + 36 G T GCGTACTTATTCGCCACCATGATTATGACCAGTGTT GCGTACTTATTCGCCACCATGATTATTACCAGTGTT -Ref 578 579 + 5386 HWI-EAS91_1_306UPAAXX:6:1:317:1411 8 9 + 36 G T GTACGCTGGACTTTGTGGGATACCCTCGCTTTCCT GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCT -Ref 2992 2993 + 5386 HWI-EAS91_1_306UPAAXX:6:1:458:1629 23 24 + 36 G T GCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGT GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT -Ref 4783 4784 + 5386 HWI-EAS91_1_306UPAAXX:6:1:646:59 18 19 - 36 C T CATTTCCATGCGGTGCACTTTATGCGGACACTTCC CATTTCCATGCGGTGCATTTTATGCGGACACTTCC -Ref 1649 1650 + 5386 HWI-EAS91_1_306UPAAXX:6:1:295:694 24 25 + 36 A G GTGATTACTTCATGCAGCGTTACCATGATGTTATTT GTGATTACTTCATGCAGCGTTACCGTGATGTTATTT -Ref 1995 1996 + 5386 HWI-EAS91_1_306UPAAXX:6:1:590:331 14 15 + 36 G T GAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGAT GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT -Ref 3261 3262 + 5386 HWI-EAS91_1_306UPAAXX:6:1:221:1238 17 18 - 36 C A AAGTCTGCCGCTGATAAAGGAAAGGATAC AAGTCTGCCGATGATAAAGGAAAGGATAC -Ref 4109 4110 + 5386 HWI-EAS91_1_306UPAAXX:6:1:478:252 12 13 - 36 C A ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC ATGCTTGGCTTCAATAAGCAGATGGATAACCGCATC -Ref 2972 2973 + 5386 HWI-EAS91_1_306UPAAXX:6:1:836:1204 17 18 + 36 G T TTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAAT TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT -Ref 2234 2235 + 5386 HWI-EAS91_1_306UPAAXX:6:1:172:1412 13 14 - 36 C A AAATTTAATGTGACCGTTTATCGCAATCTGCCGACC AAATTTAATGTGAACGTTTATCGCAATCTGCCGACC -Ref 2902 2903 + 5386 HWI-EAS91_1_306UPAAXX:6:1:540:1415 18 19 + 36 G T TTATTAAAGAGATTATTTGTCTCCAGCCACTTA TTATTAAAGAGATTATTTTTCTCCAGCCACTTA -Ref 2837 2838 + 5386 HWI-EAS91_1_306UPAAXX:6:1:253:1162 14 15 - 36 T A ATGGTTTGGTCTAACTTTACCGCTACTAAATGC ATGGTTTGGTCAAACTTTACCGCTACTAAATGC -Ref 4662 4663 + 5386 HWI-EAS91_1_306UPAAXX:6:1:828:754 26 27 + 36 G T TTTGCGTGACTATTTTCGTGATATTGGTCGTATGGT TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT -Ref 4783 4784 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1566:499 9 10 + 36 C T TGCGGTGCACTTTATGCGGACACTTCCTACAGGTAG TGCGGTGCATTTTATGCGGACACTTCCTACAGGTAG -Ref 2857 2858 + 5386 HWI-EAS91_1_306UPAAXX:6:1:578:971 15 16 + 36 G T CGCTACTAAATGCCGCGGATTGGTTTCG CGCTACTAAATTCCGCGGATTGGTTTCG -Ref 3112 3113 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1721:1800 27 28 - 36 T C TCAAGGCTCTAATGTTCCTAACCC TCAAGGCTCTAATGTCCCTAACCC -Ref 4825 4826 + 5386 HWI-EAS91_1_306UPAAXX:6:1:815:1640 9 10 - 36 T G CCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCA CCCTAATTTGGGTCGTCGGGTACGCAATCGCCGCCA -Ref 4517 4518 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1485:741 19 20 - 36 G A GAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGAT GAAATCGCGTAGAGGCTTTACTATTCAGCGTTTGAT
--- a/test-data/lastz_maf.txt Tue Oct 13 12:23:37 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1982 +0,0 @@ -##maf version=1 scoring=lastz.v1.00.23 -# lastz.v1.00.23 --yasra95short --ambiguousn --identity=0..100 --census=/Users/anton/galaxy_central/database/files/000/dataset_128.dat --coverage=0 --format=maf -# -# hsp_threshold = 10 -# gapped_threshold = 14 -# x_drop = 10 -# y_drop = 14 -# gap_open_penalty = 6 -# gap_extend_penalty = 1 -# A C G T -# A 1 -7 -7 -7 -# C -7 1 -7 -7 -# G -7 -7 1 -7 -# T -7 -7 -7 1 -a score=35 -s phiX 4294 35 + 5386 CCCCCAACTTGATATTAATAACACTATAGACCACC -s read 1 35 - 36 CCCCCAACTTGATATTAATAACACTATAGACCACC - -a score=36 -s phiX 3521 36 + 5386 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC -s read 0 36 - 36 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC - -a score=36 -s phiX 228 36 + 5386 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC -s read 0 36 + 36 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC - -a score=36 -s phiX 2070 36 + 5386 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT -s read 0 36 + 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT - -a score=35 -s phiX 1066 35 + 5386 GGTCAGATTGGTCGTCTTATTACCATTTCAACTAC -s read 1 35 - 36 GGTCAGATTGGTCGTCTTATTACCATTTCAACTAC - -a score=36 -s phiX 1787 36 + 5386 GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC -s read 0 36 + 36 GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC - -a score=36 -s phiX 1192 36 + 5386 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC -s read 0 36 + 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC - -a score=33 -s phiX 4562 33 + 5386 GGTTGGTTTATCGTTTTTGACACTCTCACGTTG -s read 0 33 + 36 GGTTGGTTTATCGTTTTTGACACTCTCACGTTG - -a score=36 -s phiX 2667 36 + 5386 GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC -s read 0 36 + 36 GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC - -a score=36 -s phiX 4247 36 + 5386 GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC -s read 0 36 + 36 GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC - -a score=36 -s phiX 2862 36 + 5386 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT -s read 0 36 + 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT - -a score=36 -s phiX 2556 36 + 5386 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC -s read 0 36 + 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC - -a score=34 -s phiX 2510 34 + 5386 GTCAACGTTATATTTTGATAGTTTGACGGTTAAT -s read 0 34 + 36 GTCAACGTTATATTTTGATAGTTTGACGGTTAAT - -a score=36 -s phiX 5151 36 + 5386 GGATGAAAATGCTCACAATGACAAATCTGTCCACGG -s read 0 36 + 36 GGATGAAAATGCTCACAATGACAAATCTGTCCACGG - -a score=36 -s phiX 1589 36 + 5386 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC -s read 0 36 - 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC - -a score=36 -s phiX 4883 36 + 5386 GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG -s read 0 36 + 36 GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG - -a score=36 -s phiX 5051 36 + 5386 GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA -s read 0 36 + 36 GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA - -a score=36 -s phiX 4908 36 + 5386 GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT -s read 0 36 + 36 GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT - -a score=36 -s phiX 2862 36 + 5386 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT -s read 0 36 + 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT - -a score=30 -s phiX 4172 30 + 5386 GTTGAGTTCGATAATGGTGATATGTATGTT -s read 0 30 + 36 GTTGAGTTCGATAATGGTGATATGTATGTT - -a score=36 -s phiX 1700 36 + 5386 GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT -s read 0 36 + 36 GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT - -a score=36 -s phiX 1682 36 + 5386 CTTATGACGCTGACAACCGTCCTTTACTTGTCATGC -s read 0 36 - 36 CTTATGACGCTGACAACCGTCCTTTACTTGTCATGC - -a score=35 -s phiX 3125 35 + 5386 GAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGC -s read 1 35 - 36 GAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGC - -a score=36 -s phiX 1664 36 + 5386 CATTTGGAGGTAAAACCTCTTATGACGCTGACAACC -s read 0 36 - 36 CATTTGGAGGTAAAACCTCTTATGACGCTGACAACC - -a score=36 -s phiX 3212 36 + 5386 GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT -s read 0 36 + 36 GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT - -a score=36 -s phiX 3419 36 + 5386 GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG -s read 0 36 + 36 GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG - -a score=36 -s phiX 512 36 + 5386 GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC -s read 0 36 + 36 GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC - -a score=33 -s phiX 2881 33 + 5386 AGGTTATTAAAGAGATTATTTGTCTCCAGCCAC -s read 3 33 - 36 AGGTTATTAAAGAGATTATTTGTCTCCAGCCAC - -a score=36 -s phiX 4470 36 + 5386 GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC -s read 0 36 + 36 GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC - -a score=36 -s phiX 1228 36 + 5386 GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT -s read 0 36 + 36 GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT - -a score=36 -s phiX 614 36 + 5386 GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT -s read 0 36 + 36 GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT - -a score=36 -s phiX 256 36 + 5386 GCACGTTCGTCAAGGACTGGTTTAGATATGAGTCAC -s read 0 36 - 36 GCACGTTCGTCAAGGACTGGTTTAGATATGAGTCAC - -a score=36 -s phiX 2380 36 + 5386 TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTC -s read 0 36 - 36 TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTC - -a score=36 -s phiX 4097 36 + 5386 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC -s read 0 36 - 36 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC - -a score=36 -s phiX 5098 36 + 5386 GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG -s read 0 36 + 36 GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG - -a score=36 -s phiX 4253 36 + 5386 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT -s read 0 36 + 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT - -a score=36 -s phiX 3407 36 + 5386 GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG -s read 0 36 + 36 GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG - -a score=36 -s phiX 4042 36 + 5386 GGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTC -s read 0 36 - 36 GGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTC - -a score=36 -s phiX 4559 36 + 5386 GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG -s read 0 36 + 36 GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG - -a score=36 -s phiX 1887 36 + 5386 GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC -s read 0 36 + 36 GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC - -a score=35 -s phiX 3433 35 + 5386 GCAAAATGAGACTCAAAAAGAGATTGCTGGCATTC -s read 1 35 - 36 GCAAAATGAGACTCAAAAAGAGATTGCTGGCATTC - -a score=36 -s phiX 4538 36 + 5386 GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC -s read 0 36 + 36 GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC - -a score=36 -s phiX 1827 36 + 5386 CTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGC -s read 0 36 - 36 CTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGC - -a score=36 -s phiX 3707 36 + 5386 GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT -s read 0 36 + 36 GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT - -a score=36 -s phiX 3325 36 + 5386 GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT -s read 0 36 + 36 GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT - -a score=36 -s phiX 4175 36 + 5386 GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT -s read 0 36 + 36 GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT - -a score=36 -s phiX 21 36 + 5386 GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC -s read 0 36 + 36 GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC - -a score=36 -s phiX 2556 36 + 5386 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC -s read 0 36 - 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC - -a score=36 -s phiX 3276 36 + 5386 ATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGC -s read 0 36 - 36 ATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGC - -a score=36 -s phiX 3591 36 + 5386 AGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGC -s read 0 36 - 36 AGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGC - -a score=35 -s phiX 357 35 + 5386 GATGCTGTTCAACCACTAATAGGTAAGAAATCATG -s read 0 35 + 36 GATGCTGTTCAACCACTAATAGGTAAGAAATCATG - -a score=36 -s phiX 1774 36 + 5386 GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA -s read 0 36 + 36 GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA - -a score=36 -s phiX 3503 36 + 5386 GTATATGCACAAAATGAGATGCTTGCTTATCAACAG -s read 0 36 + 36 GTATATGCACAAAATGAGATGCTTGCTTATCAACAG - -a score=36 -s phiX 1553 36 + 5386 CGACTTCTACCACATCTATTGACATTATGGGTCTGC -s read 0 36 - 36 CGACTTCTACCACATCTATTGACATTATGGGTCTGC - -a score=36 -s phiX 1001 36 + 5386 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC -s read 0 36 - 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC - -a score=36 -s phiX 3625 36 + 5386 GCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTAC -s read 0 36 - 36 GCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTAC - -a score=36 -s phiX 3571 36 + 5386 GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC -s read 0 36 + 36 GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC - -a score=36 -s phiX 1264 36 + 5386 GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT -s read 0 36 + 36 GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT - -a score=36 -s phiX 1331 36 + 5386 GCACGATTAACCCTGATACCAATAAAATCCCTAAGC -s read 0 36 - 36 GCACGATTAACCCTGATACCAATAAAATCCCTAAGC - -a score=36 -s phiX 4253 36 + 5386 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT -s read 0 36 + 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT - -a score=36 -s phiX 4169 36 + 5386 GGCGTTGAGTTCGATAATGGTGATATGTATGTTGAC -s read 0 36 - 36 GGCGTTGAGTTCGATAATGGTGATATGTATGTTGAC - -a score=30 -s phiX 2811 30 + 5386 GTTTATGTTGGTTTCATGGTTTGGTCTAAC -s read 6 30 - 36 GTTTATGTTGGTTTCATGGTTTGGTCTAAC - -a score=36 -s phiX 3118 36 + 5386 CCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGC -s read 0 36 - 36 CCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGC - -a score=36 -s phiX 1669 36 + 5386 GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT -s read 0 36 + 36 GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT - -a score=36 -s phiX 639 36 + 5386 ATTATGTTCATCCCGTCAACATTCAAACGGCCTGTC -s read 0 36 - 36 ATTATGTTCATCCCGTCAACATTCAAACGGCCTGTC - -a score=35 -s phiX 2749 35 + 5386 GTTATTATACCGTCAAGGACTGTGTGACTATTGAC -s read 0 35 + 36 GTTATTATACCGTCAAGGACTGTGTGACTATTGAC - -a score=36 -s phiX 4605 36 + 5386 GATTAGAGGCGTTTTATGATAATCCCAATGCTTTGC -s read 0 36 - 36 GATTAGAGGCGTTTTATGATAATCCCAATGCTTTGC - -a score=36 -s phiX 3572 36 + 5386 GAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCC -s read 0 36 - 36 GAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCC - -a score=36 -s phiX 5104 36 + 5386 GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG -s read 0 36 + 36 GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG - -a score=21 -s phiX 0 21 + 5386 GAGTTTTATCGCTTCCATGAC -s read 15 21 - 36 GAGTTTTATCGCTTCCATGAC - -a score=36 -s phiX 1673 36 + 5386 GTAAAACCTCTTATGACGCTGACAACCGTCCTTTAC -s read 0 36 - 36 GTAAAACCTCTTATGACGCTGACAACCGTCCTTTAC - -a score=36 -s phiX 4094 36 + 5386 CCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC -s read 0 36 - 36 CCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC - -a score=36 -s phiX 4550 36 + 5386 GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT -s read 0 36 + 36 GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT - -a score=36 -s phiX 3283 36 + 5386 TGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGC -s read 0 36 - 36 TGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGC - -a score=20 -s phiX 2937 20 + 5386 GTGCTATTGCTGGCGGTATT -s read 0 20 + 36 GTGCTATTGCTGGCGGTATT - -a score=36 -s phiX 4004 36 + 5386 GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC -s read 0 36 + 36 GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC - -a score=36 -s phiX 2478 36 + 5386 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA -s read 0 36 + 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA - -a score=36 -s phiX 1081 36 + 5386 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC -s read 0 36 - 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC - -a score=35 -s phiX 3044 35 + 5386 GCTACCGATAACAATACTGTAGGCATGGGTGATGC -s read 1 35 - 36 GCTACCGATAACAATACTGTAGGCATGGGTGATGC - -a score=36 -s phiX 1114 36 + 5386 GGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTC -s read 0 36 - 36 GGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTC - -a score=33 -s phiX 635 33 + 5386 GCTTATTATGTTCATCCCGTCAACATTCAAACG -s read 0 33 + 36 GCTTATTATGTTCATCCCGTCAACATTCAAACG - -a score=26 -s phiX 1138 26 + 5386 GTTGGCGCTCTCCGTCTTTCTCCATT -s read 0 26 + 36 GTTGGCGCTCTCCGTCTTTCTCCATT - -a score=36 -s phiX 851 36 + 5386 CTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTC -s read 0 36 - 36 CTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTC - -a score=36 -s phiX 5271 36 + 5386 GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT -s read 0 36 + 36 GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT - -a score=30 -s phiX 4329 36 + 5386 GCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACG -s read 0 36 + 36 GCCCCGAAGGGGACNANAAATGGTTTTTAGAGAACG - -a score=36 -s phiX 4890 36 + 5386 GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT -s read 0 36 + 36 GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT - -a score=34 -s phiX 4784 34 + 5386 TTTATGCGGACACTTCCTACAGGTAGCGTTGACC -s read 2 34 - 36 TTTATGCGGACACTTCCTACAGGTAGCGTTGACC - -a score=36 -s phiX 3618 36 + 5386 GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT -s read 0 36 + 36 GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT - -a score=32 -s phiX 4454 32 + 5386 GGTATTAAGGATGAGTGTTCAAGATTGCTGGA -s read 0 32 + 36 GGTATTAAGGATGAGTGTTCAAGATTGCTGGA - -a score=36 -s phiX 631 36 + 5386 CATTGCTTATTATGTTCATCCCGTCAACATTCAAAC -s read 0 36 - 36 CATTGCTTATTATGTTCATCCCGTCAACATTCAAAC - -a score=35 -s phiX 2963 35 + 5386 GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGG -s read 0 35 + 36 GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGG - -a score=36 -s phiX 2762 36 + 5386 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTAC -s read 0 36 - 36 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTAC - -a score=36 -s phiX 2765 36 + 5386 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC -s read 0 36 - 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC - -a score=36 -s phiX 1718 36 + 5386 GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG -s read 0 36 + 36 GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG - -a score=36 -s phiX 3099 36 + 5386 AAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCC -s read 0 36 - 36 AAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCC - -a score=29 -s phiX 597 29 + 5386 GCTTTCCTGCTCCTGTTGAGTTTATTGCT -s read 0 29 + 36 GCTTTCCTGCTCCTGTTGAGTTTATTGCT - -a score=36 -s phiX 4125 36 + 5386 ACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTC -s read 0 36 - 36 ACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTC - -a score=36 -s phiX 4025 36 + 5386 GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT -s read 0 36 + 36 GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT - -a score=36 -s phiX 2620 36 + 5386 GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT -s read 0 36 + 36 GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT - -a score=36 -s phiX 2748 36 + 5386 GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC -s read 0 36 + 36 GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC - -a score=36 -s phiX 378 36 + 5386 GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG -s read 0 36 + 36 GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG - -a score=36 -s phiX 2159 36 + 5386 GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC -s read 0 36 + 36 GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC - -a score=36 -s phiX 2872 36 + 5386 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC -s read 0 36 - 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC - -a score=35 -s phiX 1507 35 + 5386 TGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTC -s read 1 35 - 36 TGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTC - -a score=36 -s phiX 3862 36 + 5386 TTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC -s read 0 36 - 36 TTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC - -a score=36 -s phiX 5186 36 + 5386 GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC -s read 0 36 + 36 GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC - -a score=36 -s phiX 4195 36 + 5386 GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG -s read 0 36 + 36 GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG - -a score=36 -s phiX 634 36 + 5386 TGCTTATTATGTTCATCCCGTCAACATTCAAACGGC -s read 0 36 - 36 TGCTTATTATGTTCATCCCGTCAACATTCAAACGGC - -a score=35 -s phiX 1293 35 + 5386 TAACACTACTGGTTATATTGACCATGCCGCTTTTC -s read 1 35 - 36 TAACACTACTGGTTATATTGACCATGCCGCTTTTC - -a score=36 -s phiX 5156 36 + 5386 AAAATGCTCACAATGACAAATCTGTCCACGGAGTGC -s read 0 36 - 36 AAAATGCTCACAATGACAAATCTGTCCACGGAGTGC - -a score=36 -s phiX 771 36 + 5386 GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG -s read 0 36 + 36 GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG - -a score=36 -s phiX 1223 36 + 5386 GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA -s read 0 36 + 36 GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA - -a score=33 -s phiX 1543 33 + 5386 CGCCAAATGACGACTTCTACCACATCTATTGAC -s read 3 33 - 36 CGCCAAATGACGACTTCTACCACATCTATTGAC - -a score=36 -s phiX 4839 36 + 5386 GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT -s read 0 36 + 36 GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT - -a score=35 -s phiX 203 35 + 5386 GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGA -s read 0 35 + 36 GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGA - -a score=29 -s phiX 3262 29 + 5386 TGATAAAGGAAAGGATACTCGTGATTATC -s read 7 29 - 36 TGATAAAGGAAAGGATACTCGTGATTATC - -a score=28 -s phiX 2480 36 + 5386 ACCTGTTTTACAGACACCTAAAGCTACATCGTCAAC -s read 0 36 - 36 ACCTGTTTTAAAGACACCTAAAGCTACATCGTCAAC - -a score=36 -s phiX 331 36 + 5386 AAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTC -s read 0 36 - 36 AAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTC - -a score=36 -s phiX 3977 36 + 5386 TTTATGGTTCGTTCTTATTACCCTTCTGAATGTCAC -s read 0 36 - 36 TTTATGGTTCGTTCTTATTACCCTTCTGAATGTCAC - -a score=36 -s phiX 4864 36 + 5386 GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT -s read 0 36 + 36 GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT - -a score=36 -s phiX 3270 36 + 5386 GAAAGGATACTCGTGATTATCTTGCTGCTGCATTTC -s read 0 36 - 36 GAAAGGATACTCGTGATTATCTTGCTGCTGCATTTC - -a score=36 -s phiX 4173 36 + 5386 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC -s read 0 36 - 36 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC - -a score=30 -s phiX 3809 30 + 5386 GTGGTTGATATTTTTCATGGTATTGATAAA -s read 0 30 + 36 GTGGTTGATATTTTTCATGGTATTGATAAA - -a score=28 -s phiX 4634 28 + 5386 GCTTTGCGTGACTATTTTCGTGATATTG -s read 0 28 + 36 GCTTTGCGTGACTATTTTCGTGATATTG - -a score=36 -s phiX 2737 36 + 5386 GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG -s read 0 36 + 36 GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG - -a score=14 -s phiX 2191 14 + 5386 GTTCAGTTGTTGCA -s read 0 14 + 36 GTTCAGTTGTTGCA - -a score=35 -s phiX 2702 35 + 5386 CCCGACTGCCTATGATGTTTATCCTTTGAATGGTC -s read 1 35 - 36 CCCGACTGCCTATGATGTTTATCCTTTGAATGGTC - -a score=36 -s phiX 2942 36 + 5386 ATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGC -s read 0 36 - 36 ATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGC - -a score=36 -s phiX 3860 36 + 5386 AATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGC -s read 0 36 - 36 AATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGC - -a score=36 -s phiX 3092 36 + 5386 GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG -s read 0 36 + 36 GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG - -a score=36 -s phiX 3150 36 + 5386 GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG -s read 0 36 + 36 GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG - -a score=36 -s phiX 1192 36 + 5386 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC -s read 0 36 + 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC - -a score=36 -s phiX 156 36 + 5386 CGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGC -s read 0 36 - 36 CGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGC - -a score=36 -s phiX 4791 36 + 5386 GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG -s read 0 36 + 36 GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG - -a score=36 -s phiX 3848 36 + 5386 GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT -s read 0 36 + 36 GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT - -a score=35 -s phiX 2742 35 + 5386 GATGGTGGTTATTATACCGTCAAGGACTGTGTGAC -s read 1 35 - 36 GATGGTGGTTATTATACCGTCAAGGACTGTGTGAC - -a score=36 -s phiX 3014 36 + 5386 GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC -s read 0 36 + 36 GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC - -a score=36 -s phiX 1090 36 + 5386 ATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTC -s read 0 36 - 36 ATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTC - -a score=35 -s phiX 797 35 + 5386 GACGCAGAAGAAAACGTGCGTCAAAAATTACGTGC -s read 1 35 - 36 GACGCAGAAGAAAACGTGCGTCAAAAATTACGTGC - -a score=36 -s phiX 4865 36 + 5386 CAAAATACGTGGCCTTATGGTTACAGTATGCCCATC -s read 0 36 - 36 CAAAATACGTGGCCTTATGGTTACAGTATGCCCATC - -a score=36 -s phiX 4403 36 + 5386 GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT -s read 0 36 + 36 GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT - -a score=36 -s phiX 1654 36 + 5386 GTTATTTCTTCATTTGGAGGTAAAACCTCTTATGAC -s read 0 36 - 36 GTTATTTCTTCATTTGGAGGTAAAACCTCTTATGAC - -a score=36 -s phiX 5161 36 + 5386 GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT -s read 0 36 + 36 GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT - -a score=35 -s phiX 1248 35 + 5386 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTC -s read 1 35 - 36 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTC - -a score=36 -s phiX 3260 36 + 5386 GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT -s read 0 36 + 36 GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT - -a score=36 -s phiX 3095 36 + 5386 ATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCC -s read 0 36 - 36 ATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCC - -a score=35 -s phiX 2811 35 + 5386 GTTTATGTTGGTTTCATGGTTTGGTCTAACTTTAC -s read 1 35 - 36 GTTTATGTTGGTTTCATGGTTTGGTCTAACTTTAC - -a score=30 -s phiX 80 30 + 5386 GAATTACTACTGCTTGTTTACGAATTAAAT -s read 0 30 + 36 GAATTACTACTGCTTGTTTACGAATTAAAT - -a score=36 -s phiX 3458 36 + 5386 GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG -s read 0 36 + 36 GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG - -a score=35 -s phiX 788 35 + 5386 GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAA -s read 0 35 + 36 GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAA - -a score=36 -s phiX 3698 36 + 5386 GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT -s read 0 36 + 36 GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT - -a score=31 -s phiX 2640 31 + 5386 GCCGACCCTAAATTTTTTGCCTGTTTGGTTC -s read 0 31 + 36 GCCGACCCTAAATTTTTTGCCTGTTTGGTTC - -a score=36 -s phiX 304 36 + 5386 GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG -s read 0 36 + 36 GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG - -a score=35 -s phiX 3065 35 + 5386 GGCATGGGTGATGCTGGTATTAAATCTGCCATTCA -s read 0 35 + 36 GGCATGGGTGATGCTGGTATTAAATCTGCCATTCA - -a score=36 -s phiX 4427 36 + 5386 GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG -s read 0 36 + 36 GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG - -a score=36 -s phiX 1460 36 + 5386 AAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATC -s read 0 36 - 36 AAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATC - -a score=36 -s phiX 3999 36 + 5386 CTTCTGAATGTCACGCTGATTATTTTGACTTTGAGC -s read 0 36 - 36 CTTCTGAATGTCACGCTGATTATTTTGACTTTGAGC - -a score=36 -s phiX 219 36 + 5386 GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT -s read 0 36 + 36 GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT - -a score=33 -s phiX 3193 33 + 5386 GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTT -s read 0 33 + 36 GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTT - -a score=32 -s phiX 1265 32 + 5386 GTGTTAATGCCACTCCTCTCCCGACTGTTAAC -s read 0 32 + 36 GTGTTAATGCCACTCCTCTCCCGACTGTTAAC - -a score=36 -s phiX 4595 36 + 5386 GCTGACGACCGATTAGAGGCGTTTTATGATAATCCC -s read 0 36 - 36 GCTGACGACCGATTAGAGGCGTTTTATGATAATCCC - -a score=35 -s phiX 1171 35 + 5386 GGCCTTGCTATTGACTCTACTGTAGACATTTTTAC -s read 1 35 - 36 GGCCTTGCTATTGACTCTACTGTAGACATTTTTAC - -a score=36 -s phiX 635 36 + 5386 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC -s read 0 36 - 36 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC - -a score=36 -s phiX 2872 36 + 5386 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC -s read 0 36 - 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC - -a score=36 -s phiX 1013 36 + 5386 AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCC -s read 0 36 - 36 AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCC - -a score=36 -s phiX 2688 36 + 5386 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT -s read 0 36 + 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT - -a score=28 -s phiX 2147 36 + 5386 GCGTACTTATTCGCCACCATGATTATGACCAGTGTT -s read 0 36 + 36 GCGTACTTATTCGCCACCATGATTATTACCAGTGTT - -a score=35 -s phiX 5190 35 + 5386 GCTTAATCCAACTTACCAAGCTGGGTTACGACGCG -s read 0 35 + 36 GCTTAATCCAACTTACCAAGCTGGGTTACGACGCG - -a score=36 -s phiX 1589 36 + 5386 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC -s read 0 36 - 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC - -a score=27 -s phiX 570 35 + 5386 GTACGCTGGACTTTGTGGGATACCCTCGCTTTCCT -s read 0 35 + 36 GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCT - -a score=36 -s phiX 241 36 + 5386 GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT -s read 0 36 + 36 GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT - -a score=36 -s phiX 337 36 + 5386 GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC -s read 0 36 + 36 GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC - -a score=30 -s phiX 1111 30 + 5386 GCTGGCGACTCCTTCGAGATGGACGCCGTT -s read 0 30 + 36 GCTGGCGACTCCTTCGAGATGGACGCCGTT - -a score=34 -s phiX 1253 34 + 5386 TCATGAAGGATGGTGTTAATGCCACTCCTCTCCC -s read 2 34 - 36 TCATGAAGGATGGTGTTAATGCCACTCCTCTCCC - -a score=18 -s phiX 3730 18 + 5386 GAATCAGCGGTATGGCTC -s read 0 18 + 36 GAATCAGCGGTATGGCTC - -a score=28 -s phiX 2969 36 + 5386 GCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGT -s read 0 36 + 36 GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT - -a score=36 -s phiX 5150 36 + 5386 GGGATGAAAATGCTCACAATGACAAATCTGTCCACG -s read 0 36 + 36 GGGATGAAAATGCTCACAATGACAAATCTGTCCACG - -a score=36 -s phiX 347 36 + 5386 ATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAA -s read 0 36 - 36 ATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAA - -a score=35 -s phiX 4887 35 + 5386 ACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC -s read 1 35 - 36 ACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC - -a score=33 -s phiX 3971 33 + 5386 GGCTTTTTTATGGTTCGTTCTTATTACCCTTCT -s read 0 33 + 36 GGCTTTTTTATGGTTCGTTCTTATTACCCTTCT - -a score=36 -s phiX 5032 36 + 5386 GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC -s read 0 36 + 36 GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC - -a score=35 -s phiX 1551 35 + 5386 GACGACTTCTACCACATCTATTGACATTATGGGTC -s read 1 35 - 36 GACGACTTCTACCACATCTATTGACATTATGGGTC - -a score=36 -s phiX 1763 36 + 5386 AAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAAC -s read 0 36 - 36 AAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAAC - -a score=36 -s phiX 1528 36 + 5386 GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC -s read 0 36 + 36 GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC - -a score=36 -s phiX 4949 36 + 5386 CCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACC -s read 0 36 - 36 CCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACC - -a score=34 -s phiX 4483 34 + 5386 GGAGGCCTCCACTATGAAATCGCGTAGAGGCTTT -s read 0 34 - 36 GGAGGCCTCCACTATGAAATCGCGTAGAGGCTTT - -a score=27 -s phiX 4766 35 + 5386 CATTTCCATGCGGTGCACTTTATGCGGACACTTCC -s read 1 35 - 36 CATTTCCATGCGGTGCATTTTATGCGGACACTTCC - -a score=36 -s phiX 176 36 + 5386 CTTTGCGACCTTTCGCCATCAACTAACGATTCTGTC -s read 0 36 - 36 CTTTGCGACCTTTCGCCATCAACTAACGATTCTGTC - -a score=36 -s phiX 2576 36 + 5386 GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT -s read 0 36 + 36 GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT - -a score=36 -s phiX 155 36 + 5386 GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG -s read 0 36 + 36 GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG - -a score=36 -s phiX 226 36 + 5386 TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT -s read 0 36 + 36 TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT - -a score=36 -s phiX 573 36 + 5386 CGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC -s read 0 36 - 36 CGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC - -a score=36 -s phiX 3020 36 + 5386 GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC -s read 0 36 + 36 GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC - -a score=36 -s phiX 3730 36 + 5386 GAATCAGCGGTATGGCTCTTCTCATATTGGCGCTAC -s read 0 36 - 36 GAATCAGCGGTATGGCTCTTCTCATATTGGCGCTAC - -a score=36 -s phiX 2435 36 + 5386 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC -s read 0 36 + 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC - -a score=36 -s phiX 2709 36 + 5386 GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT -s read 0 36 + 36 GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT - -a score=28 -s phiX 4227 28 + 5386 TTCGTGATGAGTTTGTATCTGTTACTGA -s read 0 28 + 36 TTCGTGATGAGTTTGTATCTGTTACTGA - -a score=34 -s phiX 4605 34 + 5386 GATTAGAGGCGTTTTATGATAATCCCAATGCTTT -s read 0 34 + 36 GATTAGAGGCGTTTTATGATAATCCCAATGCTTT - -a score=36 -s phiX 2765 36 + 5386 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC -s read 0 36 - 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC - -a score=34 -s phiX 581 34 + 5386 TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTG -s read 0 34 + 36 TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTG - -a score=26 -s phiX 663 26 + 5386 AAACGGCCTGTCTCATCATGGAAGGC -s read 10 26 - 36 AAACGGCCTGTCTCATCATGGAAGGC - -a score=36 -s phiX 1143 36 + 5386 CGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC -s read 0 36 - 36 CGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC - -a score=36 -s phiX 1513 36 + 5386 GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA -s read 0 36 + 36 GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA - -a score=26 -s phiX 4035 26 + 5386 GTATCGAGGCTCTTAAACCTGCTATT -s read 0 26 + 36 GTATCGAGGCTCTTAAACCTGCTATT - -a score=36 -s phiX 1656 36 + 5386 TATTTCTTCATTTGGAGGTAAAACCTCTTATGACGC -s read 0 36 - 36 TATTTCTTCATTTGGAGGTAAAACCTCTTATGACGC - -a score=36 -s phiX 2070 36 + 5386 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT -s read 0 36 + 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT - -a score=28 -s phiX 1625 36 + 5386 GTGATTACTTCATGCAGCGTTACCATGATGTTATTT -s read 0 36 + 36 GTGATTACTTCATGCAGCGTTACCGTGATGTTATTT - -a score=36 -s phiX 3249 36 + 5386 GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG -s read 0 36 + 36 GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG - -a score=28 -s phiX 1981 36 + 5386 GAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGAT -s read 0 36 + 36 GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT - -a score=36 -s phiX 2216 36 + 5386 AGGTTAAATTTAATGTGACCGTTTATCGCAATCTGC -s read 0 36 - 36 AGGTTAAATTTAATGTGACCGTTTATCGCAATCTGC - -a score=29 -s phiX 3485 29 + 5386 CAGAATACGAAAGACCAGGTATATGCACA -s read 7 29 - 36 CAGAATACGAAAGACCAGGTATATGCACA - -a score=36 -s phiX 1689 36 + 5386 CGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAA -s read 0 36 - 36 CGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAA - -a score=21 -s phiX 3251 29 + 5386 AAGTCTGCCGCTGATAAAGGAAAGGATAC -s read 7 29 - 36 AAGTCTGCCGATGATAAAGGAAAGGATAC - -a score=36 -s phiX 1081 36 + 5386 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC -s read 0 36 - 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC - -a score=36 -s phiX 4281 36 + 5386 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA -s read 0 36 - 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA - -a score=34 -s phiX 4135 34 + 5386 GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG -s read 0 34 + 36 GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG - -a score=36 -s phiX 4413 36 + 5386 TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA -s read 0 36 + 36 TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA - -a score=36 -s phiX 3987 36 + 5386 GTTCTTATTACCCTTCTGAATGTCACGCTGATTATT -s read 0 36 - 36 GTTCTTATTACCCTTCTGAATGTCACGCTGATTATT - -a score=36 -s phiX 4202 36 + 5386 GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG -s read 0 36 + 36 GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG - -a score=28 -s phiX 4097 36 + 5386 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC -s read 0 36 - 36 ATGCTTGGCTTCAATAAGCAGATGGATAACCGCATC - -a score=35 -s phiX 4306 35 + 5386 TATTAATAACACTATAGACCACCGCCCCGAAGGGG -s read 0 35 + 36 TATTAATAACACTATAGACCACCGCCCCGAAGGGG - -a score=35 -s phiX 1520 35 + 5386 TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACG -s read 0 35 + 36 TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACG - -a score=36 -s phiX 944 36 + 5386 TAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC -s read 0 36 - 36 TAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC - -a score=36 -s phiX 1574 36 + 5386 ACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC -s read 0 36 - 36 ACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC - -a score=36 -s phiX 2688 36 + 5386 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT -s read 0 36 + 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT - -a score=36 -s phiX 2098 36 + 5386 GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT -s read 0 36 + 36 GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT - -a score=36 -s phiX 523 36 + 5386 TACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGC -s read 0 36 - 36 TACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGC - -a score=36 -s phiX 1251 36 + 5386 GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC -s read 0 36 + 36 GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC - -a score=33 -s phiX 4915 33 + 5386 GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTG -s read 0 33 + 36 GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTG - -a score=29 -s phiX 3827 29 + 5386 GGTATTGATAAAGCTGTTGCCGATACTTG -s read 0 29 + 36 GGTATTGATAAAGCTGTTGCCGATACTTG - -a score=36 -s phiX 3755 36 + 5386 ATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTC -s read 0 36 - 36 ATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTC - -a score=26 -s phiX 663 26 + 5386 AAACGGCCTGTCTCATCATGGAAGGC -s read 10 26 - 36 AAACGGCCTGTCTCATCATGGAAGGC - -a score=36 -s phiX 1115 36 + 5386 GCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCC -s read 0 36 - 36 GCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCC - -a score=28 -s phiX 3098 28 + 5386 CAAGGCTCTAATGTTCCTAACCCTGATG -s read 0 28 - 36 CAAGGCTCTAATGTTCCTAACCCTGATG - -a score=36 -s phiX 3081 36 + 5386 GTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTA -s read 0 36 - 36 GTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTA - -a score=27 -s phiX 4220 27 + 5386 TCTGACGTTCGTGATGAGTTTGTATCT -s read 0 27 + 36 TCTGACGTTCGTGATGAGTTTGTATCT - -a score=36 -s phiX 612 36 + 5386 TTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC -s read 0 36 - 36 TTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC - -a score=36 -s phiX 4935 36 + 5386 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC -s read 0 36 - 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC - -a score=36 -s phiX 288 36 + 5386 TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC -s read 0 36 + 36 TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC - -a score=36 -s phiX 4689 36 + 5386 GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT -s read 0 36 + 36 GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT - -a score=23 -s phiX 2546 23 + 5386 TGGTAATGGTGGTTTTCTTCATT -s read 0 23 + 36 TGGTAATGGTGGTTTTCTTCATT - -a score=36 -s phiX 3519 36 + 5386 AGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTC -s read 0 36 - 36 AGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTC - -a score=36 -s phiX 3857 36 + 5386 AACAATTTCTGGAAAGACGGTAAAGCTGATGGTATT -s read 0 36 - 36 AACAATTTCTGGAAAGACGGTAAAGCTGATGGTATT - -a score=36 -s phiX 3343 36 + 5386 TTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAA -s read 0 36 - 36 TTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAA - -a score=27 -s phiX 4671 27 + 5386 TTCTTGCTGCCGAGGGTCGCAAGGCTA -s read 0 27 + 36 TTCTTGCTGCCGAGGGTCGCAAGGCTA - -a score=31 -s phiX 1345 31 + 5386 GATACCAATAAAATCCCTAAGCATTTGTTTC -s read 0 31 + 36 GATACCAATAAAATCCCTAAGCATTTGTTTC - -a score=36 -s phiX 1799 36 + 5386 AGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTC -s read 0 36 - 36 AGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTC - -a score=36 -s phiX 5076 36 + 5386 GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC -s read 0 36 + 36 GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC - -a score=35 -s phiX 2762 35 + 5386 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA -s read 1 35 - 36 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA - -a score=36 -s phiX 4281 36 + 5386 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA -s read 0 36 + 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA - -a score=27 -s phiX 4655 27 + 5386 GATATTGGTCGTATGGTTCTTGCTGCC -s read 0 27 + 36 GATATTGGTCGTATGGTTCTTGCTGCC - -a score=18 -s phiX 4934 18 + 5386 TCTGGTTGGTTGTGGCCT -s read 0 18 + 36 TCTGGTTGGTTGTGGCCT - -a score=33 -s phiX 1202 33 + 5386 TTACTTTTTATGTCCCTCATCGTCACGTTTATG -s read 0 33 + 36 TTACTTTTTATGTCCCTCATCGTCACGTTTATG - -a score=36 -s phiX 5106 36 + 5386 TACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCC -s read 0 36 - 36 TACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCC - -a score=35 -s phiX 5167 35 + 5386 AATGACAAATCTGTCCACGGAGTGCTTAATCCAAC -s read 1 35 - 36 AATGACAAATCTGTCCACGGAGTGCTTAATCCAAC - -a score=28 -s phiX 2955 36 + 5386 TTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAAT -s read 0 36 + 36 TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT - -a score=30 -s phiX 623 30 + 5386 GCTGCCGTCATTGCTTATTATGTTCATCCC -s read 0 30 + 36 GCTGCCGTCATTGCTTATTATGTTCATCCC - -a score=35 -s phiX 1876 35 + 5386 CCGCCTACTGCGACTAAAGAGATTCAGTACCTTAA -s read 1 35 - 36 CCGCCTACTGCGACTAAAGAGATTCAGTACCTTAA - -a score=36 -s phiX 1213 36 + 5386 GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT -s read 0 36 + 36 GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT - -a score=27 -s phiX 557 27 + 5386 GCTTGCGTTTATGGTACGCTGGACTTT -s read 0 27 + 36 GCTTGCGTTTATGGTACGCTGGACTTT - -a score=36 -s phiX 3628 36 + 5386 TACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAA -s read 0 36 - 36 TACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAA - -a score=35 -s phiX 2253 35 + 5386 GACCACTCGCGATTCAATCATGACTTCGTGATAAA -s read 0 35 + 36 GACCACTCGCGATTCAATCATGACTTCGTGATAAA - -a score=36 -s phiX 2478 36 + 5386 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA -s read 0 36 + 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA - -a score=36 -s phiX 3000 36 + 5386 GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG -s read 0 36 + 36 GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG - -a score=35 -s phiX 4666 35 + 5386 TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATG -s read 0 35 + 36 TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATG - -a score=36 -s phiX 3463 36 + 5386 CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGA -s read 0 36 - 36 CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGA - -a score=35 -s phiX 4910 35 + 5386 TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTT -s read 0 35 + 36 TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTT - -a score=29 -s phiX 4591 29 + 5386 GTTGGCTGACGACCGATTAGAGGCGTTTT -s read 0 29 + 36 GTTGGCTGACGACCGATTAGAGGCGTTTT - -a score=28 -s phiX 2221 36 + 5386 AAATTTAATGTGACCGTTTATCGCAATCTGCCGACC -s read 0 36 - 36 AAATTTAATGTGAACGTTTATCGCAATCTGCCGACC - -a score=35 -s phiX 645 35 + 5386 TTCATCCCGTCAACATTCAAACGGCCTGTCTCATC -s read 0 35 + 36 TTCATCCCGTCAACATTCAAACGGCCTGTCTCATC - -a score=36 -s phiX 1363 36 + 5386 AAGCATTTGTTTCAGGGTTATTTGAATATCTATAAC -s read 0 36 - 36 AAGCATTTGTTTCAGGGTTATTTGAATATCTATAAC - -a score=35 -s phiX 1612 35 + 5386 ACTGACCAAGAACGTGATTACTTCATGCAGCGTTA -s read 1 35 - 36 ACTGACCAAGAACGTGATTACTTCATGCAGCGTTA - -a score=35 -s phiX 1044 35 + 5386 TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC -s read 0 35 + 36 TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC - -a score=25 -s phiX 2884 33 + 5386 TTATTAAAGAGATTATTTGTCTCCAGCCACTTA -s read 0 33 + 36 TTATTAAAGAGATTATTTTTCTCCAGCCACTTA - -a score=36 -s phiX 1587 36 + 5386 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA -s read 0 36 + 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA - -a score=36 -s phiX 338 36 + 5386 TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT -s read 0 36 + 36 TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT - -a score=36 -s phiX 4936 36 + 5386 TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC -s read 0 36 + 36 TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC - -a score=34 -s phiX 4343 34 + 5386 GAAAAATGGTTTTTAGAGAACGAGAAGACGGTTA -s read 2 34 - 36 GAAAAATGGTTTTTAGAGAACGAGAAGACGGTTA - -a score=36 -s phiX 3673 36 + 5386 CAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGA -s read 0 36 - 36 CAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGA - -a score=36 -s phiX 4944 36 + 5386 TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG -s read 0 36 + 36 TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG - -a score=36 -s phiX 1945 36 + 5386 GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT -s read 0 36 + 36 GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT - -a score=32 -s phiX 2661 32 + 5386 TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCC -s read 0 32 + 36 TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCC - -a score=36 -s phiX 3312 36 + 5386 TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT -s read 0 36 + 36 TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT - -a score=25 -s phiX 2826 33 + 5386 ATGGTTTGGTCTAACTTTACCGCTACTAAATGC -s read 3 33 - 36 ATGGTTTGGTCAAACTTTACCGCTACTAAATGC - -a score=36 -s phiX 726 36 + 5386 GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT -s read 0 36 + 36 GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT - -a score=36 -s phiX 554 36 + 5386 GAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGA -s read 0 36 - 36 GAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGA - -a score=36 -s phiX 484 36 + 5386 TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT -s read 0 36 + 36 TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT - -a score=36 -s phiX 317 36 + 5386 TGTTGACATTTTAAAAGAGCGTGGATTACTATCTGA -s read 0 36 - 36 TGTTGACATTTTAAAAGAGCGTGGATTACTATCTGA - -a score=36 -s phiX 909 36 + 5386 TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT -s read 0 36 + 36 TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT - -a score=36 -s phiX 4935 36 + 5386 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC -s read 0 36 - 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC - -a score=36 -s phiX 5004 36 + 5386 TCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA -s read 0 36 - 36 TCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA - -a score=33 -s phiX 1378 33 + 5386 GGTTATTTGAATATCTATAACAACTATTTTAAA -s read 0 33 + 36 GGTTATTTGAATATCTATAACAACTATTTTAAA - -a score=30 -s phiX 3167 30 + 5386 GGTAAAGGACTTCTTGAAGGTACGTTGCAG -s read 0 30 + 36 GGTAAAGGACTTCTTGAAGGTACGTTGCAG - -a score=33 -s phiX 2737 33 + 5386 GCCATGATGGTGGTTATTATACCGTCAAGGACT -s read 0 33 + 36 GCCATGATGGTGGTTATTATACCGTCAAGGACT - -a score=36 -s phiX 4098 36 + 5386 TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA -s read 0 36 + 36 TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA - -a score=36 -s phiX 2573 36 + 5386 TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT -s read 0 36 + 36 TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT - -a score=36 -s phiX 2452 36 + 5386 TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT -s read 0 36 + 36 TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT - -a score=36 -s phiX 5178 36 + 5386 TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG -s read 0 36 + 36 TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG - -a score=30 -s phiX 3777 30 + 5386 TTTCTAATGTCGTCACTGATGCTGCTTCTG -s read 0 30 + 36 TTTCTAATGTCGTCACTGATGCTGCTTCTG - -a score=36 -s phiX 1842 36 + 5386 TGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCC -s read 0 36 - 36 TGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCC - -a score=36 -s phiX 1081 36 + 5386 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC -s read 0 36 - 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC - -a score=36 -s phiX 1727 36 + 5386 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC -s read 0 36 - 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC - -a score=28 -s phiX 4636 36 + 5386 TTTGCGTGACTATTTTCGTGATATTGGTCGTATGGT -s read 0 36 + 36 TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT - -a score=36 -s phiX 2224 36 + 5386 TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT -s read 0 36 + 36 TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT - -a score=36 -s phiX 3367 36 + 5386 CGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCA -s read 0 36 - 36 CGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCA - -a score=36 -s phiX 3310 36 + 5386 GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC -s read 0 36 + 36 GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC - -a score=36 -s phiX 5038 36 + 5386 TATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGA -s read 0 36 - 36 TATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGA - -a score=36 -s phiX 2058 36 + 5386 TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT -s read 0 36 + 36 TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT - -a score=36 -s phiX 387 36 + 5386 TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC -s read 0 36 + 36 TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC - -a score=36 -s phiX 2753 36 + 5386 TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT -s read 0 36 + 36 TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT - -a score=36 -s phiX 2127 36 + 5386 TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCA -s read 0 36 - 36 TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCA - -a score=36 -s phiX 153 36 + 5386 TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT -s read 0 36 + 36 TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT - -a score=36 -s phiX 3616 36 + 5386 GCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCA -s read 0 36 - 36 GCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCA - -a score=36 -s phiX 2727 36 + 5386 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC -s read 0 36 - 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC - -a score=36 -s phiX 2774 36 + 5386 GACTATTGACGTCCTTCCCCGTACGCCGGGCAATAA -s read 0 36 - 36 GACTATTGACGTCCTTCCCCGTACGCCGGGCAATAA - -a score=36 -s phiX 4216 36 + 5386 TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC -s read 0 36 + 36 TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC - -a score=35 -s phiX 4618 35 + 5386 TTATGATAATCCCAATGCTTTGCGTGACTATTTTC -s read 0 35 + 36 TTATGATAATCCCAATGCTTTGCGTGACTATTTTC - -a score=35 -s phiX 410 35 + 5386 TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA -s read 0 35 + 36 TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA - -a score=35 -s phiX 2729 35 + 5386 GAATGGTCGCCATGATGGTGGTTATTATACCGTCA -s read 0 35 + 36 GAATGGTCGCCATGATGGTGGTTATTATACCGTCA - -a score=35 -s phiX 520 35 + 5386 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTG -s read 0 35 + 36 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTG - -a score=36 -s phiX 4259 36 + 5386 TTAATGGATGAATTGGCACAATGCTACAATGTGCTC -s read 0 36 + 36 TTAATGGATGAATTGGCACAATGCTACAATGTGCTC - -a score=36 -s phiX 1661 36 + 5386 CTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA -s read 0 36 - 36 CTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA - -a score=36 -s phiX 532 36 + 5386 CTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTA -s read 0 36 - 36 CTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTA - -a score=35 -s phiX 194 35 + 5386 TCAACTAACGATTCTGTCAAAAACTGACGCGTTGG -s read 0 35 + 36 TCAACTAACGATTCTGTCAAAAACTGACGCGTTGG - -a score=36 -s phiX 2238 36 + 5386 TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT -s read 0 36 + 36 TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT - -a score=33 -s phiX 1312 33 + 5386 GACCATGCCGCTTTTCTTGGCACGATTAACCCT -s read 0 33 + 36 GACCATGCCGCTTTTCTTGGCACGATTAACCCT - -a score=36 -s phiX 1544 36 + 5386 GCCAAATGACGACTTCTACCACATCTATTGACATTA -s read 0 36 - 36 GCCAAATGACGACTTCTACCACATCTATTGACATTA - -a score=36 -s phiX 556 36 + 5386 GGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATA -s read 0 36 - 36 GGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATA - -a score=36 -s phiX 1248 36 + 5386 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT -s read 0 36 + 36 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT - -a score=33 -s phiX 4173 33 + 5386 TTGAGTTCGATAATGGTGATATGTATGTTGACG -s read 0 33 + 36 TTGAGTTCGATAATGGTGATATGTATGTTGACG - -a score=35 -s phiX 613 35 + 5386 TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC -s read 0 35 + 36 TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC - -a score=36 -s phiX 205 36 + 5386 TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT -s read 0 36 + 36 TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT - -a score=36 -s phiX 4980 36 + 5386 CTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTA -s read 0 36 - 36 CTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTA - -a score=30 -s phiX 1701 30 + 5386 TCCTTTACTTGTCATGCGCTCTAATCTCTG -s read 0 30 + 36 TCCTTTACTTGTCATGCGCTCTAATCTCTG - -a score=35 -s phiX 2320 35 + 5386 TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACC -s read 0 35 + 36 TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACC - -a score=19 -s phiX 4183 19 + 5386 TAATGGTGATATGTATGTT -s read 0 19 + 36 TAATGGTGATATGTATGTT - -a score=35 -s phiX 4087 35 + 5386 TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGG -s read 0 35 + 36 TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGG - -a score=36 -s phiX 899 36 + 5386 TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC -s read 0 36 + 36 TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC - -a score=36 -s phiX 1154 36 + 5386 TTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA -s read 0 36 - 36 TTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA - -a score=36 -s phiX 3586 36 + 5386 TTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCA -s read 0 36 - 36 TTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCA - -a score=36 -s phiX 3786 36 + 5386 TCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTT -s read 0 36 - 36 TCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTT - -a score=35 -s phiX 3833 35 + 5386 GATAAAGCTGTTGCCGATACTTGGAACAATTTCTG -s read 0 35 + 36 GATAAAGCTGTTGCCGATACTTGGAACAATTTCTG - -a score=36 -s phiX 988 36 + 5386 TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC -s read 0 36 + 36 TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC - -a score=29 -s phiX 5357 29 + 5386 ATAAAAATGATTGGCGTATCCAACCTGCA -s read 0 29 + 36 ATAAAAATGATTGGCGTATCCAACCTGCA - -a score=36 -s phiX 3711 36 + 5386 TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT -s read 0 36 + 36 TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT - -a score=36 -s phiX 1206 36 + 5386 TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA -s read 0 36 + 36 TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA - -a score=21 -s phiX 4299 21 + 5386 AACTTGATATTAATAACACTA -s read 15 21 - 36 AACTTGATATTAATAACACTA - -a score=36 -s phiX 2115 36 + 5386 TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT -s read 0 36 + 36 TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT - -a score=36 -s phiX 659 36 + 5386 ATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAA -s read 0 36 - 36 ATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAA - -a score=31 -s phiX 1806 31 + 5386 TAAACATTCTGTGCCGCGTTTCTTTGTTCCT -s read 0 31 + 36 TAAACATTCTGTGCCGCGTTTCTTTGTTCCT - -a score=36 -s phiX 1535 36 + 5386 AGCTTTCTCGCCAAATGACGACTTCTACCACATCTA -s read 0 36 - 36 AGCTTTCTCGCCAAATGACGACTTCTACCACATCTA - -a score=35 -s phiX 2111 35 + 5386 TCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAA -s read 1 35 - 36 TCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAA - -a score=36 -s phiX 2815 36 + 5386 ATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTA -s read 0 36 - 36 ATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTA - -a score=36 -s phiX 5175 36 + 5386 ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC -s read 0 36 + 36 ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC - -a score=36 -s phiX 3471 36 + 5386 CGGCGACTTCACGCCAGAATACGAAAGACCAGGTAT -s read 0 36 - 36 CGGCGACTTCACGCCAGAATACGAAAGACCAGGTAT - -a score=36 -s phiX 1947 36 + 5386 CGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGA -s read 0 36 - 36 CGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGA - -a score=35 -s phiX 2211 35 + 5386 TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCA -s read 0 35 + 36 TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCA - -a score=35 -s phiX 3816 35 + 5386 ATATTTTTCATGGTATTGATAAAGCTGTTGCCGAT -s read 0 35 + 36 ATATTTTTCATGGTATTGATAAAGCTGTTGCCGAT - -a score=36 -s phiX 3562 36 + 5386 GTCTATTATGGAAAACACCAATCTTTCCAAGCAACA -s read 0 36 - 36 GTCTATTATGGAAAACACCAATCTTTCCAAGCAACA - -a score=35 -s phiX 4197 35 + 5386 ATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGT -s read 1 35 - 36 ATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGT - -a score=35 -s phiX 2906 35 + 5386 CCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGC -s read 1 35 - 36 CCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGC - -a score=35 -s phiX 2842 35 + 5386 TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTG -s read 0 35 + 36 TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTG - -a score=31 -s phiX 4373 31 + 5386 GTTACGCAGTTTTGCCGCAAGCTGGCTGCTG -s read 0 31 + 36 GTTACGCAGTTTTGCCGCAAGCTGGCTGCTG - -a score=16 -s phiX 431 16 + 5386 TTGGCCTCTATTAAGC -s read 10 16 - 36 TTGGCCTCTATTAAGC - -a score=35 -s phiX 1939 35 + 5386 ATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCC -s read 1 35 - 36 ATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCC - -a score=36 -s phiX 5072 36 + 5386 GAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA -s read 0 36 - 36 GAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA - -a score=36 -s phiX 4433 36 + 5386 TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG -s read 0 36 + 36 TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG - -a score=35 -s phiX 1273 35 + 5386 GCCACTCCTCTCCCGACTGTTAACACTACTGGTTA -s read 1 35 - 36 GCCACTCCTCTCCCGACTGTTAACACTACTGGTTA - -a score=36 -s phiX 4439 36 + 5386 TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA -s read 0 36 + 36 TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA - -a score=36 -s phiX 3919 36 + 5386 GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT -s read 0 36 + 36 GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT - -a score=35 -s phiX 4586 35 + 5386 CTCACGTTGGCTGACGACCGATTAGAGGCGTTTTA -s read 1 35 - 36 CTCACGTTGGCTGACGACCGATTAGAGGCGTTTTA - -a score=24 -s phiX 3729 24 + 5386 AGAATCAGCGGTATGGCTCTTCTC -s read 0 24 + 36 AGAATCAGCGGTATGGCTCTTCTC - -a score=36 -s phiX 1706 36 + 5386 TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT -s read 0 36 + 36 TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT - -a score=36 -s phiX 1581 36 + 5386 GGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGA -s read 0 36 - 36 GGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGA - -a score=36 -s phiX 1587 36 + 5386 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA -s read 0 36 - 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA - -a score=34 -s phiX 1179 34 + 5386 TATTGACTCTACTGTAGACATTTTTACTTTTTAT -s read 0 34 + 36 TATTGACTCTACTGTAGACATTTTTACTTTTTAT - -a score=36 -s phiX 2697 36 + 5386 ACCCTCCCGACTGCCTATGATGTTTATCCTTTGAAT -s read 0 36 - 36 ACCCTCCCGACTGCCTATGATGTTTATCCTTTGAAT - -a score=36 -s phiX 3166 36 + 5386 TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG -s read 0 36 + 36 TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG - -a score=25 -s phiX 2497 25 + 5386 CTAAAGCTACATCGTCAACGTTATA -s read 11 25 - 36 CTAAAGCTACATCGTCAACGTTATA - -a score=36 -s phiX 2288 36 + 5386 AGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAA -s read 0 36 - 36 AGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAA - -a score=36 -s phiX 440 36 + 5386 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA -s read 0 36 - 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA - -a score=36 -s phiX 1011 36 + 5386 TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC -s read 0 36 + 36 TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC - -a score=36 -s phiX 160 36 + 5386 GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC -s read 0 36 + 36 GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC - -a score=35 -s phiX 1164 35 + 5386 GCGTCGTGGCCTTGCTATTGACTCTACTGTAGACA -s read 1 35 - 36 GCGTCGTGGCCTTGCTATTGACTCTACTGTAGACA - -a score=31 -s phiX 5014 31 + 5386 TAAATACGTTAACAAAAAGTCAGATATGGAC -s read 5 31 - 36 TAAATACGTTAACAAAAAGTCAGATATGGAC - -a score=36 -s phiX 2285 36 + 5386 AAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGT -s read 0 36 - 36 AAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGT - -a score=32 -s phiX 800 32 + 5386 GCAGAAGAAAACGTGCGTCAAAAATTACGTGC -s read 0 32 - 36 GCAGAAGAAAACGTGCGTCAAAAATTACGTGC - -a score=36 -s phiX 1586 36 + 5386 TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG -s read 0 36 + 36 TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG - -a score=36 -s phiX 4577 36 + 5386 TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG -s read 0 36 + 36 TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG - -a score=36 -s phiX 3569 36 + 5386 ATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTT -s read 0 36 - 36 ATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTT - -a score=36 -s phiX 495 36 + 5386 TTCTGACGAGTAACAAAGTTTGGATTGCTACTGACC -s read 0 36 - 36 TTCTGACGAGTAACAAAGTTTGGATTGCTACTGACC - -a score=36 -s phiX 440 36 + 5386 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA -s read 0 36 + 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA - -a score=30 -s phiX 4753 30 + 5386 TAATGGCCGTCTTCATTTCCATGCGGTGCA -s read 0 30 - 36 TAATGGCCGTCTTCATTTCCATGCGGTGCA - -a score=35 -s phiX 4234 35 + 5386 TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATG -s read 0 35 + 36 TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATG - -a score=36 -s phiX 2292 36 + 5386 TGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATT -s read 0 36 - 36 TGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATT - -a score=35 -s phiX 1578 35 + 5386 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA -s read 1 35 - 36 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA - -a score=31 -s phiX 3331 31 + 5386 TGGTGCTGATGCTTCCTCTGCTGGTATGGTT -s read 0 31 + 36 TGGTGCTGATGCTTCCTCTGCTGGTATGGTT - -a score=36 -s phiX 4131 36 + 5386 TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC -s read 0 36 + 36 TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC - -a score=28 -s phiX 4774 36 + 5386 TGCGGTGCACTTTATGCGGACACTTCCTACAGGTAG -s read 0 36 + 36 TGCGGTGCATTTTATGCGGACACTTCCTACAGGTAG - -a score=16 -s phiX 650 24 + 5386 CCCGTCAACATTCAAACGGCCTGT -s read 12 24 - 36 CCCGTAAACATTCAAACGGCCTGT - -a score=32 -s phiX 0 32 + 5386 GAGTTTTATCGCTTCCATGACGCAGAAGTTAA -s read 4 32 - 36 GAGTTTTATCGCTTCCATGACGCAGAAGTTAA - -a score=36 -s phiX 53 36 + 5386 AGTCGAAAAATTATCTTGATAAAGCAGGAATTACTA -s read 0 36 - 36 AGTCGAAAAATTATCTTGATAAAGCAGGAATTACTA - -a score=35 -s phiX 679 35 + 5386 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA -s read 1 35 - 36 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA - -a score=36 -s phiX 230 36 + 5386 TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT -s read 0 36 + 36 TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT - -a score=36 -s phiX 4191 36 + 5386 ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG -s read 0 36 + 36 ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG - -a score=36 -s phiX 1579 36 + 5386 ATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACT -s read 0 36 - 36 ATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACT - -a score=36 -s phiX 610 36 + 5386 TGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGT -s read 0 36 - 36 TGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGT - -a score=35 -s phiX 4531 35 + 5386 GATGAATGCAATGCGACAGGCTCATGCTGATGGTT -s read 1 35 - 36 GATGAATGCAATGCGACAGGCTCATGCTGATGGTT - -a score=30 -s phiX 637 30 + 5386 TTATTATGTTCATCCCGTCAACATTCAAAC -s read 0 30 + 36 TTATTATGTTCATCCCGTCAACATTCAAAC - -a score=20 -s phiX 2846 28 + 5386 CGCTACTAAATGCCGCGGATTGGTTTCG -s read 4 28 + 36 CGCTACTAAATTCCGCGGATTGGTTTCG - -a score=36 -s phiX 1956 36 + 5386 TTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTAT -s read 0 36 - 36 TTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTAT - -a score=36 -s phiX 2727 36 + 5386 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC -s read 0 36 - 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC - -a score=36 -s phiX 3978 36 + 5386 TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG -s read 0 36 + 36 TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG - -a score=35 -s phiX 539 35 + 5386 GCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTAC -s read 1 35 - 36 GCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTAC - -a score=36 -s phiX 1851 36 + 5386 GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGC -s read 0 36 - 36 GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGC - -a score=36 -s phiX 4683 36 + 5386 AGGGTCGCAAGGCTAATGATTCACACGCCGACTGCT -s read 0 36 - 36 AGGGTCGCAAGGCTAATGATTCACACGCCGACTGCT - -a score=32 -s phiX 3689 32 + 5386 AAGGTTAGTGCTGAGGTTGACTTAGTTCATCA -s read 0 32 + 36 AAGGTTAGTGCTGAGGTTGACTTAGTTCATCA - -a score=36 -s phiX 583 36 + 5386 TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT -s read 0 36 + 36 TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT - -a score=35 -s phiX 3851 35 + 5386 ACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGA -s read 1 35 - 36 ACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGA - -a score=35 -s phiX 2856 35 + 5386 TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA -s read 0 35 + 36 TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA - -a score=26 -s phiX 3781 26 + 5386 TAATGTCGTCACTGATGCTGCTTCTG -s read 0 26 + 36 TAATGTCGTCACTGATGCTGCTTCTG - -a score=36 -s phiX 1727 36 + 5386 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC -s read 0 36 + 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC - -a score=35 -s phiX 4657 35 + 5386 TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCA -s read 0 35 + 36 TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCA - -a score=36 -s phiX 1720 36 + 5386 TCTAATCTCTGGGCATCTGGCTATGATGTTGATGGA -s read 0 36 - 36 TCTAATCTCTGGGCATCTGGCTATGATGTTGATGGA - -a score=36 -s phiX 1953 36 + 5386 TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC -s read 0 36 + 36 TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC - -a score=36 -s phiX 961 36 + 5386 TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA -s read 0 36 + 36 TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA - -a score=36 -s phiX 2174 36 + 5386 ACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGA -s read 0 36 - 36 ACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGA - -a score=36 -s phiX 2441 36 + 5386 TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC -s read 0 36 + 36 TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC - -a score=35 -s phiX 5125 35 + 5386 CAGAATCAGAATGAGCCGCAACTTCGGGATGAAAA -s read 1 35 - 36 CAGAATCAGAATGAGCCGCAACTTCGGGATGAAAA - -a score=36 -s phiX 5205 36 + 5386 CCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGA -s read 0 36 - 36 CCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGA - -a score=36 -s phiX 2878 36 + 5386 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC -s read 0 36 + 36 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC - -a score=36 -s phiX 2003 36 + 5386 TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG -s read 0 36 + 36 TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG - -a score=36 -s phiX 2153 36 + 5386 TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT -s read 0 36 + 36 TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT - -a score=35 -s phiX 1092 35 + 5386 TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCG -s read 0 35 + 36 TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCG - -a score=36 -s phiX 3126 36 + 5386 AGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTA -s read 0 36 - 36 AGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTA - -a score=36 -s phiX 3543 36 + 5386 AGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACA -s read 0 36 - 36 AGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACA - -a score=31 -s phiX 2507 31 + 5386 ATCGTCAACGTTATATTTTGATAGTTTGACG -s read 0 31 + 36 ATCGTCAACGTTATATTTTGATAGTTTGACG - -a score=16 -s phiX 3097 24 + 5386 TCAAGGCTCTAATGTTCCTAACCC -s read 12 24 - 36 TCAAGGCTCTAATGTCCCTAACCC - -a score=36 -s phiX 3599 36 + 5386 CAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAA -s read 0 36 - 36 CAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAA - -a score=36 -s phiX 1733 36 + 5386 CATCTGGCTATGATGTTGATGGAACTGACCAAACGT -s read 0 36 - 36 CATCTGGCTATGATGTTGATGGAACTGACCAAACGT - -a score=33 -s phiX 2654 33 + 5386 TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTC -s read 0 33 + 36 TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTC - -a score=36 -s phiX 2933 36 + 5386 TTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTT -s read 0 36 - 36 TTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTT - -a score=36 -s phiX 1521 36 + 5386 TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC -s read 0 36 + 36 TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC - -a score=36 -s phiX 4062 36 + 5386 AGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAA -s read 0 36 - 36 AGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAA - -a score=36 -s phiX 3394 36 + 5386 TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA -s read 0 36 + 36 TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA - -a score=36 -s phiX 408 36 + 5386 AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA -s read 0 36 + 36 AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA - -a score=36 -s phiX 1866 36 + 5386 TGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCA -s read 0 36 - 36 TGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCA - -a score=33 -s phiX 3779 33 + 5386 TCTAATGTCGTCACTGATGCTGCTTCTGGTGTG -s read 0 33 + 36 TCTAATGTCGTCACTGATGCTGCTTCTGGTGTG - -a score=36 -s phiX 3981 36 + 5386 TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG -s read 0 36 + 36 TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG - -a score=36 -s phiX 2814 36 + 5386 TATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCT -s read 0 36 - 36 TATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCT - -a score=36 -s phiX 1241 36 + 5386 AGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCA -s read 0 36 - 36 AGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCA - -a score=35 -s phiX 4658 35 + 5386 ATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAA -s read 1 35 - 36 ATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAA - -a score=36 -s phiX 2435 36 + 5386 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC -s read 0 36 + 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC - -a score=35 -s phiX 526 35 + 5386 TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTT -s read 0 35 + 36 TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTT - -a score=28 -s phiX 4816 36 + 5386 CCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCA -s read 0 36 - 36 CCCTAATTTGGGTCGTCGGGTACGCAATCGCCGCCA - -a score=36 -s phiX 2099 36 + 5386 AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG -s read 0 36 + 36 AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG - -a score=36 -s phiX 2761 36 + 5386 TCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA -s read 0 36 - 36 TCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA - -a score=36 -s phiX 4954 36 + 5386 TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA -s read 0 36 + 36 TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA - -a score=36 -s phiX 3760 36 + 5386 CGCTACTGCAAAGGATATTTCTAATGTCGTCACTGA -s read 0 36 - 36 CGCTACTGCAAAGGATATTTCTAATGTCGTCACTGA - -a score=28 -s phiX 4498 36 + 5386 GAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGAT -s read 0 36 - 36 GAAATCGCGTAGAGGCTTTACTATTCAGCGTTTGAT - -a score=36 -s phiX 4038 36 + 5386 TCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCA -s read 0 36 - 36 TCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCA - -a score=36 -s phiX 4787 36 + 5386 ATGCGGACACTTCCTACAGGTAGCGTTGACCCTAAT -s read 0 36 - 36 ATGCGGACACTTCCTACAGGTAGCGTTGACCCTAAT - -a score=36 -s phiX 1001 36 + 5386 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC -s read 0 36 + 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC - -a score=36 -s phiX 4380 36 + 5386 AGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTA -s read 0 36 - 36 AGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTA - -a score=36 -s phiX 4255 36 + 5386 GAAGTTAATGGATGAATTGGCACAATGCTACAATGT -s read 0 36 - 36 GAAGTTAATGGATGAATTGGCACAATGCTACAATGT - -a score=35 -s phiX 9 35 + 5386 CGCTTCCATGACGCAGAAGTTAACACTTTCGGATA -s read 1 35 - 36 CGCTTCCATGACGCAGAAGTTAACACTTTCGGATA - -a score=36 -s phiX 1578 36 + 5386 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATAC -s read 0 36 - 36 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATAC - -a score=30 -s phiX 988 30 + 5386 TGAGGATAAATTATGTCTAATATTCAAACT -s read 0 30 + 36 TGAGGATAAATTATGTCTAATATTCAAACT - -a score=36 -s phiX 3861 36 + 5386 ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT -s read 0 36 + 36 ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT - -a score=36 -s phiX 3277 36 + 5386 TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT -s read 0 36 + 36 TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT - -a score=36 -s phiX 348 36 + 5386 TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG -s read 0 36 + 36 TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG - -a score=36 -s phiX 4607 36 + 5386 TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT -s read 0 36 + 36 TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT - -a score=35 -s phiX 3807 35 + 5386 GTGTGGTTGATATTTTTCATGGTATTGATAAAGCT -s read 0 35 + 36 GTGTGGTTGATATTTTTCATGGTATTGATAAAGCT - -a score=31 -s phiX 4752 31 + 5386 CTAATGGCCGTCTTCATTTCCATGCGGTGCA -s read 0 31 - 36 CTAATGGCCGTCTTCATTTCCATGCGGTGCA - -a score=36 -s phiX 285 36 + 5386 GAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTT -s read 0 36 - 36 GAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTT - -a score=36 -s phiX 2849 36 + 5386 TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT -s read 0 36 + 36 TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT - -a score=35 -s phiX 679 35 + 5386 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA -s read 1 35 - 36 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA - -a score=36 -s phiX 4739 36 + 5386 GAGTATGGTACAGCTAATGGCCGTCTTCATTTCCAT -s read 0 36 - 36 GAGTATGGTACAGCTAATGGCCGTCTTCATTTCCAT - -a score=25 -s phiX 4654 25 + 5386 TGATATTGGTCGTATGGTTCTTGCT -s read 0 25 + 36 TGATATTGGTCGTATGGTTCTTGCT - -a score=35 -s phiX 2466 35 + 5386 CCAGCTTCTTCGGCACCTGTTTTACAGACACCTAA -s read 1 35 - 36 CCAGCTTCTTCGGCACCTGTTTTACAGACACCTAA - -a score=34 -s phiX 319 34 + 5386 TTGACATTTTAAAAGAGCGTGGATTACTATCTGA -s read 0 34 + 36 TTGACATTTTAAAAGAGCGTGGATTACTATCTGA - -a score=34 -s phiX 1179 34 + 5386 TATTGACTCTACTGTAGACATTTTTACTTTTTAT -s read 0 34 + 36 TATTGACTCTACTGTAGACATTTTTACTTTTTAT - -a score=36 -s phiX 520 36 + 5386 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGA -s read 0 36 - 36 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGA - -a score=36 -s phiX 332 36 + 5386 AGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCA -s read 0 36 - 36 AGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCA - -a score=36 -s phiX 2755 36 + 5386 ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC -s read 0 36 + 36 ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC - -a score=36 -s phiX 4956 36 + 5386 ATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATA -s read 0 36 - 36 ATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATA - -a score=36 -s phiX 71 36 + 5386 ATAAAGCAGGAATTACTACTGCTTGTTTACGAATTA -s read 0 36 - 36 ATAAAGCAGGAATTACTACTGCTTGTTTACGAATTA - -a score=36 -s phiX 4248 36 + 5386 TTACTGAGAAGTTAATGGATGAATTGGCACAATGCT -s read 0 36 - 36 TTACTGAGAAGTTAATGGATGAATTGGCACAATGCT - -a score=36 -s phiX 2591 36 + 5386 CAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA -s read 0 36 - 36 CAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA - -a score=36 -s phiX 1973 36 + 5386 CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTT -s read 0 36 - 36 CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTT - -a score=36 -s phiX 3539 36 + 5386 AAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAA -s read 0 36 - 36 AAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAA - -a score=36 -s phiX 4129 36 + 5386 CATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTAT -s read 0 36 - 36 CATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTAT -
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/lastz_seqs.loc Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,31 @@ +#This is a sample file distributed with Galaxy that enables tools +#to use a directory of 2bit genome files for use with Lastz. You will +#need to supply these files and then create a lastz_seqs.loc file +#similar to this one (store it in this directory) that points to +#the directories in which those files are stored. The lastz_seqs.loc +#file has this format (white space characters are TAB characters): +# +#<unique_build_id> <display_name> <file_path> +# +#So, for example, if your lastz_seqs.loc began like this: +# +#hg18 Human (Homo sapiens): hg18 /depot/data2/galaxy/twobit/hg18.2bit +#hg19 Human (Homo sapiens): hg19 /depot/data2/galaxy/twobit/hg19.2bit +#mm9 Mouse (Mus musculus): mm9 /depot/data2/galaxy/twobit/mm9.2bit +# +#then your /depot/data2/galaxy/twobit/ directory +#would need to contain the following 2bit files: +# +#-rw-r--r-- 1 james universe 830134 2005-09-13 10:12 hg18.2bit +#-rw-r--r-- 1 james universe 527388 2005-09-13 10:12 hg19.2bit +#-rw-r--r-- 1 james universe 269808 2005-09-13 10:12 mm9.2bit +# +#Your lastz_seqs.loc file should include an entry per line for +#each file you have stored that you want to be available. Note that +#your files should all have the extension '2bit'. +# +#Note that for backwards compatibility with workflows, the unique ID of +#an entry must be the path that was in the original loc file, because that +#is the value stored in the workflow for that parameter. +# +phiX174 test 2 bit file (phiX174) ${__HERE__}/phiX.2bit
--- a/test-data/lastz_wrapper_out2.sam Tue Oct 13 12:23:37 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,459 +0,0 @@ -HWI-EAS91_1_306UPAAXX 16 phiX174 4295 255 1H35M * 0 0 CCCCCAACTTGATATTAATAACACTATAGACCACC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3522 255 36M * 0 0 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 229 255 36M * 0 0 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2071 255 36M * 0 0 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1067 255 1H35M * 0 0 GGTCAGATTGGTCGTCTTATTACCATTTCAACTAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1788 255 36M * 0 0 GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1193 255 36M * 0 0 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4563 255 36M * 0 0 GGTTGGTTTATCGTTTTTGACACTCTCACGTTGTCT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2668 255 36M * 0 0 GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4248 255 36M * 0 0 GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2863 255 36M * 0 0 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2557 255 36M * 0 0 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2511 255 34M2H * 0 0 GTCAACGTTATATTTTGATAGTTTGACGGTTAAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 5152 255 36M * 0 0 GGATGAAAATGCTCACAATGACAAATCTGTCCACGG * -HWI-EAS91_1_306UPAAXX 16 phiX174 1590 255 36M * 0 0 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4884 255 36M * 0 0 GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG * -HWI-EAS91_1_306UPAAXX 0 phiX174 5052 255 36M * 0 0 GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4909 255 36M * 0 0 GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2863 255 36M * 0 0 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT * -HWI-EAS91_1_306UPAAXX 0 phiX174 4173 255 36M * 0 0 GTTGAGTTCGATAATGGTGATATGTATGTTTACGGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1701 255 36M * 0 0 GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1683 255 36M * 0 0 CTTATGACGCTGACAACCGTCCTTTACTTGTCATGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3126 255 1H35M * 0 0 GAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1665 255 36M * 0 0 CATTTGGAGGTAAAACCTCTTATGACGCTGACAACC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3213 255 36M * 0 0 GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3420 255 36M * 0 0 GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG * -HWI-EAS91_1_306UPAAXX 0 phiX174 513 255 36M * 0 0 GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2879 255 36M * 0 0 ATAAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4471 255 36M * 0 0 GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1229 255 36M * 0 0 GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 615 255 36M * 0 0 GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 257 255 36M * 0 0 GCACGTTCGTCAAGGACTGGTTTAGATATGAGTCAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2381 255 36M * 0 0 TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4098 255 36M * 0 0 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC * -HWI-EAS91_1_306UPAAXX 0 phiX174 5099 255 36M * 0 0 GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG * -HWI-EAS91_1_306UPAAXX 0 phiX174 4254 255 36M * 0 0 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3408 255 36M * 0 0 GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG * -HWI-EAS91_1_306UPAAXX 16 phiX174 4043 255 36M * 0 0 GGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4560 255 36M * 0 0 GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG * -HWI-EAS91_1_306UPAAXX 0 phiX174 1888 255 36M * 0 0 GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3434 255 1H35M * 0 0 GCAAAATGAGACTCAAAAAGAGATTGCTGGCATTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4539 255 36M * 0 0 GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1828 255 36M * 0 0 CTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3708 255 36M * 0 0 GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3326 255 36M * 0 0 GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 4176 255 36M * 0 0 GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 22 255 36M * 0 0 GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2557 255 36M * 0 0 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3277 255 36M * 0 0 ATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3592 255 36M * 0 0 AGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 358 255 35M1H * 0 0 GATGCTGTTCAACCACTAATAGGTAAGAAATCATG * -HWI-EAS91_1_306UPAAXX 0 phiX174 1775 255 36M * 0 0 GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 3504 255 36M * 0 0 GTATATGCACAAAATGAGATGCTTGCTTATCAACAG * -HWI-EAS91_1_306UPAAXX 16 phiX174 1554 255 36M * 0 0 CGACTTCTACCACATCTATTGACATTATGGGTCTGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1002 255 36M * 0 0 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3626 255 36M * 0 0 GCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3572 255 36M * 0 0 GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1265 255 36M * 0 0 GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1332 255 36M * 0 0 GCACGATTAACCCTGATACCAATAAAATCCCTAAGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4254 255 36M * 0 0 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 4170 255 36M * 0 0 GGCGTTGAGTTCGATAATGGTGATATGTATGTTGAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2806 255 36M * 0 0 AATAATGTTTATGTTGGTTTCATGGTTTGGTCTAAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3119 255 36M * 0 0 CCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1670 255 36M * 0 0 GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 640 255 36M * 0 0 ATTATGTTCATCCCGTCAACATTCAAACGGCCTGTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2750 255 35M1H * 0 0 GTTATTATACCGTCAAGGACTGTGTGACTATTGAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4606 255 36M * 0 0 GATTAGAGGCGTTTTATGATAATCCCAATGCTTTGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3573 255 36M * 0 0 GAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 5105 255 36M * 0 0 GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG * -HWI-EAS91_1_306UPAAXX 16 phiX174 1674 255 36M * 0 0 GTAAAACCTCTTATGACGCTGACAACCGTCCTTTAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4095 255 36M * 0 0 CCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4551 255 36M * 0 0 GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT * -HWI-EAS91_1_306UPAAXX 16 phiX174 3284 255 36M * 0 0 TGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4005 255 36M * 0 0 GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2479 255 36M * 0 0 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1082 255 36M * 0 0 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3045 255 1H35M * 0 0 GCTACCGATAACAATACTGTAGGCATGGGTGATGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1115 255 36M * 0 0 GGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 636 255 36M * 0 0 GCTTATTATGTTCATCCCGTCAACATTCAAACGTCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1139 255 34M2H * 0 0 GTTGGCGCTCTCCGTCTTTCTCCATTTCGTCGTG * -HWI-EAS91_1_306UPAAXX 16 phiX174 852 255 36M * 0 0 CTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 5272 255 36M * 0 0 GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 4330 255 36M * 0 0 GCCCCGAAGGGGACNANAAATGGTTTTTAGAGAACG * -HWI-EAS91_1_306UPAAXX 0 phiX174 4891 255 36M * 0 0 GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT * -HWI-EAS91_1_306UPAAXX 16 phiX174 4783 255 36M * 0 0 ATTTTATGCGGACACTTCCTACAGGTAGCGTTGACC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3619 255 36M * 0 0 GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 4455 255 36M * 0 0 GGTATTAAGGATGAGTGTTCAAGATTGCTGGATGCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 632 255 36M * 0 0 CATTGCTTATTATGTTCATCCCGTCAACATTCAAAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2964 255 35M1H * 0 0 GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGG * -HWI-EAS91_1_306UPAAXX 16 phiX174 2763 255 36M * 0 0 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2766 255 36M * 0 0 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1719 255 36M * 0 0 GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG * -HWI-EAS91_1_306UPAAXX 16 phiX174 3100 255 36M * 0 0 AAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 598 255 35M1H * 0 0 GCTTTCCTGCTCCTGTTGAGTTTATTGCTTCCGTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4126 255 36M * 0 0 ACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4026 255 36M * 0 0 GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2621 255 36M * 0 0 GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2749 255 36M * 0 0 GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 379 255 36M * 0 0 GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG * -HWI-EAS91_1_306UPAAXX 0 phiX174 2160 255 36M * 0 0 GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2873 255 36M * 0 0 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1508 255 1H35M * 0 0 TGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3863 255 36M * 0 0 TTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 5187 255 36M * 0 0 GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4196 255 36M * 0 0 GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG * -HWI-EAS91_1_306UPAAXX 16 phiX174 635 255 36M * 0 0 TGCTTATTATGTTCATCCCGTCAACATTCAAACGGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1294 255 1H35M * 0 0 TAACACTACTGGTTATATTGACCATGCCGCTTTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 5157 255 36M * 0 0 AAAATGCTCACAATGACAAATCTGTCCACGGAGTGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 772 255 36M * 0 0 GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG * -HWI-EAS91_1_306UPAAXX 0 phiX174 1224 255 36M * 0 0 GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1544 255 3H33M * 0 0 CGCCAAATGACGACTTCTACCACATCTATTGAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4840 255 36M * 0 0 GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 204 255 35M1H * 0 0 GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGA * -HWI-EAS91_1_306UPAAXX 16 phiX174 2481 255 36M * 0 0 ACCTGTTTTAAAGACACCTAAAGCTACATCGTCAAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 332 255 36M * 0 0 AAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3978 255 36M * 0 0 TTTATGGTTCGTTCTTATTACCCTTCTGAATGTCAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4865 255 36M * 0 0 GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 3271 255 36M * 0 0 GAAAGGATACTCGTGATTATCTTGCTGCTGCATTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4174 255 36M * 0 0 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2738 255 36M * 0 0 GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG * -HWI-EAS91_1_306UPAAXX 16 phiX174 2703 255 1H35M * 0 0 CCCGACTGCCTATGATGTTTATCCTTTGAATGGTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2943 255 36M * 0 0 ATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3861 255 36M * 0 0 AATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3093 255 36M * 0 0 GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG * -HWI-EAS91_1_306UPAAXX 0 phiX174 3151 255 36M * 0 0 GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG * -HWI-EAS91_1_306UPAAXX 0 phiX174 1193 255 36M * 0 0 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 157 255 36M * 0 0 CGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4792 255 36M * 0 0 GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG * -HWI-EAS91_1_306UPAAXX 0 phiX174 3849 255 36M * 0 0 GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 2743 255 1H35M * 0 0 GATGGTGGTTATTATACCGTCAAGGACTGTGTGAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3015 255 36M * 0 0 GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1091 255 36M * 0 0 ATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 798 255 1H35M * 0 0 GACGCAGAAGAAAACGTGCGTCAAAAATTACGTGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4866 255 36M * 0 0 CAAAATACGTGGCCTTATGGTTACAGTATGCCCATC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4404 255 36M * 0 0 GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1655 255 36M * 0 0 GTTATTTCTTCATTTGGAGGTAAAACCTCTTATGAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 5162 255 36M * 0 0 GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1249 255 1H35M * 0 0 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3261 255 36M * 0 0 GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 3096 255 36M * 0 0 ATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2811 255 36M * 0 0 TGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3459 255 36M * 0 0 GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG * -HWI-EAS91_1_306UPAAXX 0 phiX174 789 255 35M1H * 0 0 GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 3699 255 36M * 0 0 GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2641 255 36M * 0 0 GCCGACCCTAAATTTTTTGCCTGTTTGGTTCTCTTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 305 255 36M * 0 0 GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG * -HWI-EAS91_1_306UPAAXX 0 phiX174 3066 255 35M1H * 0 0 GGCATGGGTGATGCTGGTATTAAATCTGCCATTCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4428 255 36M * 0 0 GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG * -HWI-EAS91_1_306UPAAXX 16 phiX174 1461 255 36M * 0 0 AAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4000 255 36M * 0 0 CTTCTGAATGTCACGCTGATTATTTTGACTTTGAGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 220 255 36M * 0 0 GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3194 255 36M * 0 0 GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTTCT * -HWI-EAS91_1_306UPAAXX 0 phiX174 1266 255 35M1H * 0 0 GTGTTAATGCCACTCCTCTCCCGACTGTTAACTCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 4596 255 36M * 0 0 GCTGACGACCGATTAGAGGCGTTTTATGATAATCCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1172 255 1H35M * 0 0 GGCCTTGCTATTGACTCTACTGTAGACATTTTTAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 636 255 36M * 0 0 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2873 255 36M * 0 0 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1014 255 36M * 0 0 AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2689 255 36M * 0 0 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2148 255 36M * 0 0 GCGTACTTATTCGCCACCATGATTATTACCAGTGTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 5191 255 35M1H * 0 0 GCTTAATCCAACTTACCAAGCTGGGTTACGACGCG * -HWI-EAS91_1_306UPAAXX 16 phiX174 1590 255 36M * 0 0 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 571 255 35M1H * 0 0 GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCT * -HWI-EAS91_1_306UPAAXX 0 phiX174 242 255 36M * 0 0 GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 338 255 36M * 0 0 GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1112 255 36M * 0 0 GCTGGCGACTCCTTCGAGATGGACGCCGTTTGCGCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1254 255 2H34M * 0 0 TCATGAAGGATGGTGTTAATGCCACTCCTCTCCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2970 255 36M * 0 0 GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 5151 255 36M * 0 0 GGGATGAAAATGCTCACAATGACAAATCTGTCCACG * -HWI-EAS91_1_306UPAAXX 16 phiX174 348 255 36M * 0 0 ATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 4888 255 1H35M * 0 0 ACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3972 255 33M3H * 0 0 GGCTTTTTTATGGTTCGTTCTTATTACCCTTCT * -HWI-EAS91_1_306UPAAXX 0 phiX174 5033 255 36M * 0 0 GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1552 255 1H35M * 0 0 GACGACTTCTACCACATCTATTGACATTATGGGTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1764 255 36M * 0 0 AAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1529 255 36M * 0 0 GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4950 255 36M * 0 0 CCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4484 255 36M * 0 0 GGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4767 255 1H35M * 0 0 CATTTCCATGCGGTGCATTTTATGCGGACACTTCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 177 255 36M * 0 0 CTTTGCGACCTTTCGCCATCAACTAACGATTCTGTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2577 255 36M * 0 0 GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 156 255 36M * 0 0 GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG * -HWI-EAS91_1_306UPAAXX 0 phiX174 227 255 36M * 0 0 TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 574 255 36M * 0 0 CGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3021 255 36M * 0 0 GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3731 255 36M * 0 0 GAATCAGCGGTATGGCTCTTCTCATATTGGCGCTAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2436 255 36M * 0 0 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2710 255 36M * 0 0 GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 4228 255 35M1H * 0 0 TTCGTGATGAGTTTGTATCTGTTACTGATAAGTTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4606 255 34M2H * 0 0 GATTAGAGGCGTTTTATGATAATCCCAATGCTTT * -HWI-EAS91_1_306UPAAXX 16 phiX174 2766 255 36M * 0 0 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 582 255 34M2H * 0 0 TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTG * -HWI-EAS91_1_306UPAAXX 16 phiX174 1144 255 36M * 0 0 CGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1514 255 36M * 0 0 GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1657 255 36M * 0 0 TATTTCTTCATTTGGAGGTAAAACCTCTTATGACGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2071 255 36M * 0 0 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 1626 255 36M * 0 0 GTGATTACTTCATGCAGCGTTACCGTGATGTTATTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3250 255 36M * 0 0 GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG * -HWI-EAS91_1_306UPAAXX 0 phiX174 1982 255 36M * 0 0 GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 2217 255 36M * 0 0 AGGTTAAATTTAATGTGACCGTTTATCGCAATCTGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3480 255 1H35M * 0 0 TCACGACAGAATACGAAAGACCAGGTATATGCACA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1690 255 36M * 0 0 CGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1082 255 36M * 0 0 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4282 255 36M * 0 0 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4136 255 34M2H * 0 0 GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG * -HWI-EAS91_1_306UPAAXX 0 phiX174 4414 255 36M * 0 0 TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 3988 255 36M * 0 0 GTTCTTATTACCCTTCTGAATGTCACGCTGATTATT * -HWI-EAS91_1_306UPAAXX 0 phiX174 4203 255 36M * 0 0 GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG * -HWI-EAS91_1_306UPAAXX 16 phiX174 4098 255 36M * 0 0 ATGCTTGGCTTCAATAAGCAGATGGATAACCGCATC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4307 255 35M1H * 0 0 TATTAATAACACTATAGACCACCGCCCCGAAGGGG * -HWI-EAS91_1_306UPAAXX 0 phiX174 1521 255 35M1H * 0 0 TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACG * -HWI-EAS91_1_306UPAAXX 16 phiX174 945 255 36M * 0 0 TAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1575 255 36M * 0 0 ACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2689 255 36M * 0 0 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2099 255 36M * 0 0 GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 524 255 36M * 0 0 TACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1252 255 36M * 0 0 GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4916 255 36M * 0 0 GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGTCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3828 255 36M * 0 0 GGTATTGATAAAGCTGTTGCCGATACTTGTAACAAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 3756 255 36M * 0 0 ATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1116 255 36M * 0 0 GCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3099 255 36M * 0 0 CAAGGCTCTAATGTTCCTAACCCTGATGGGGCCGCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3082 255 36M * 0 0 GTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 613 255 36M * 0 0 TTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4936 255 36M * 0 0 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 289 255 36M * 0 0 TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4690 255 36M * 0 0 GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 3520 255 36M * 0 0 AGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3858 255 36M * 0 0 AACAATTTCTGGAAAGACGGTAAAGCTGATGGTATT * -HWI-EAS91_1_306UPAAXX 16 phiX174 3344 255 36M * 0 0 TTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4672 255 36M * 0 0 TTCTTGCTGCCGAGGGTCGCAAGGCTATTGTTTCAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1800 255 36M * 0 0 AGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 5077 255 36M * 0 0 GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2763 255 1H35M * 0 0 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4282 255 36M * 0 0 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1203 255 36M * 0 0 TTACTTTTTATGTCCCTCATCGTCACGTTTATGTTG * -HWI-EAS91_1_306UPAAXX 16 phiX174 5107 255 36M * 0 0 TACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 5168 255 1H35M * 0 0 AATGACAAATCTGTCCACGGAGTGCTTAATCCAAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2956 255 36M * 0 0 TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 624 255 36M * 0 0 GCTGCCGTCATTGCTTATTATGTTCATCCCTTCAAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1877 255 1H35M * 0 0 CCGCCTACTGCGACTAAAGAGATTCAGTACCTTAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1214 255 36M * 0 0 GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT * -HWI-EAS91_1_306UPAAXX 0 phiX174 558 255 36M * 0 0 GCTTGCGTTTATGGTACGCTGGACTTTTTGTGATAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3629 255 36M * 0 0 TACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2254 255 35M1H * 0 0 GACCACTCGCGATTCAATCATGACTTCGTGATAAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2479 255 36M * 0 0 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 3001 255 36M * 0 0 GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG * -HWI-EAS91_1_306UPAAXX 0 phiX174 4667 255 35M1H * 0 0 TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATG * -HWI-EAS91_1_306UPAAXX 16 phiX174 3464 255 36M * 0 0 CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4911 255 35M1H * 0 0 TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTT * -HWI-EAS91_1_306UPAAXX 16 phiX174 2222 255 36M * 0 0 AAATTTAATGTGAACGTTTATCGCAATCTGCCGACC * -HWI-EAS91_1_306UPAAXX 0 phiX174 646 255 35M1H * 0 0 TTCATCCCGTCAACATTCAAACGGCCTGTCTCATC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1364 255 36M * 0 0 AAGCATTTGTTTCAGGGTTATTTGAATATCTATAAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1613 255 1H35M * 0 0 ACTGACCAAGAACGTGATTACTTCATGCAGCGTTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1045 255 35M1H * 0 0 TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 1588 255 36M * 0 0 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 339 255 36M * 0 0 TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT * -HWI-EAS91_1_306UPAAXX 0 phiX174 4937 255 36M * 0 0 TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4344 255 2H34M * 0 0 GAAAAATGGTTTTTAGAGAACGAGAAGACGGTTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 3674 255 36M * 0 0 CAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4945 255 36M * 0 0 TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG * -HWI-EAS91_1_306UPAAXX 0 phiX174 1946 255 36M * 0 0 GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2662 255 36M * 0 0 TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCTACT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3313 255 36M * 0 0 TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT * -HWI-EAS91_1_306UPAAXX 0 phiX174 727 255 36M * 0 0 GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 555 255 36M * 0 0 GAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 485 255 36M * 0 0 TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 318 255 36M * 0 0 TGTTGACATTTTAAAAGAGCGTGGATTACTATCTGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 910 255 36M * 0 0 TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 4936 255 36M * 0 0 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 5005 255 36M * 0 0 TCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1379 255 36M * 0 0 GGTTATTTGAATATCTATAACAACTATTTTAAATCG * -HWI-EAS91_1_306UPAAXX 0 phiX174 3168 255 36M * 0 0 GGTAAAGGACTTCTTGAAGGTACGTTGCAGTCTGGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2738 255 33M3H * 0 0 GCCATGATGGTGGTTATTATACCGTCAAGGACT * -HWI-EAS91_1_306UPAAXX 0 phiX174 4099 255 36M * 0 0 TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2574 255 36M * 0 0 TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2453 255 36M * 0 0 TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 5179 255 36M * 0 0 TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG * -HWI-EAS91_1_306UPAAXX 0 phiX174 3778 255 34M2H * 0 0 TTTCTAATGTCGTCACTGATGCTGCTTCTGTTGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1843 255 36M * 0 0 TGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1082 255 36M * 0 0 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1728 255 36M * 0 0 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4637 255 36M * 0 0 TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2225 255 36M * 0 0 TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT * -HWI-EAS91_1_306UPAAXX 16 phiX174 3368 255 36M * 0 0 CGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 3311 255 36M * 0 0 GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 5039 255 36M * 0 0 TATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2059 255 36M * 0 0 TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT * -HWI-EAS91_1_306UPAAXX 0 phiX174 388 255 36M * 0 0 TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2754 255 36M * 0 0 TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 2128 255 36M * 0 0 TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 154 255 36M * 0 0 TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT * -HWI-EAS91_1_306UPAAXX 16 phiX174 3617 255 36M * 0 0 GCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCA * -HWI-EAS91_1_306UPAAXX 16 phiX174 2728 255 36M * 0 0 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2775 255 36M * 0 0 GACTATTGACGTCCTTCCCCGTACGCCGGGCAATAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4217 255 36M * 0 0 TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4619 255 35M1H * 0 0 TTATGATAATCCCAATGCTTTGCGTGACTATTTTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 411 255 35M1H * 0 0 TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2730 255 35M1H * 0 0 GAATGGTCGCCATGATGGTGGTTATTATACCGTCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 521 255 35M1H * 0 0 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTG * -HWI-EAS91_1_306UPAAXX 0 phiX174 4260 255 36M * 0 0 TTAATGGATGAATTGGCACAATGCTACAATGTGCTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1662 255 36M * 0 0 CTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA * -HWI-EAS91_1_306UPAAXX 16 phiX174 533 255 36M * 0 0 CTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 195 255 35M1H * 0 0 TCAACTAACGATTCTGTCAAAAACTGACGCGTTGG * -HWI-EAS91_1_306UPAAXX 0 phiX174 2239 255 36M * 0 0 TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 1313 255 36M * 0 0 GACCATGCCGCTTTTCTTGGCACGATTAACCCTTAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1545 255 36M * 0 0 GCCAAATGACGACTTCTACCACATCTATTGACATTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 557 255 36M * 0 0 GGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1249 255 36M * 0 0 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT * -HWI-EAS91_1_306UPAAXX 0 phiX174 4174 255 36M * 0 0 TTGAGTTCGATAATGGTGATATGTATGTTGACGTCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 614 255 35M1H * 0 0 TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 206 255 36M * 0 0 TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 4981 255 36M * 0 0 CTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1702 255 36M * 0 0 TCCTTTACTTGTCATGCGCTCTAATCTCTGTGCATC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2321 255 35M1H * 0 0 TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4088 255 35M1H * 0 0 TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGG * -HWI-EAS91_1_306UPAAXX 0 phiX174 900 255 36M * 0 0 TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1155 255 36M * 0 0 TTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 3587 255 36M * 0 0 TTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCA * -HWI-EAS91_1_306UPAAXX 16 phiX174 3787 255 36M * 0 0 TCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3834 255 35M1H * 0 0 GATAAAGCTGTTGCCGATACTTGGAACAATTTCTG * -HWI-EAS91_1_306UPAAXX 0 phiX174 989 255 36M * 0 0 TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3712 255 36M * 0 0 TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT * -HWI-EAS91_1_306UPAAXX 0 phiX174 1207 255 36M * 0 0 TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2116 255 36M * 0 0 TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 660 255 36M * 0 0 ATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1536 255 36M * 0 0 AGCTTTCTCGCCAAATGACGACTTCTACCACATCTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 2112 255 1H35M * 0 0 TCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 2816 255 36M * 0 0 ATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 5176 255 36M * 0 0 ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 3472 255 36M * 0 0 CGGCGACTTCACGCCAGAATACGAAAGACCAGGTAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1948 255 36M * 0 0 CGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2212 255 35M1H * 0 0 TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 3817 255 35M1H * 0 0 ATATTTTTCATGGTATTGATAAAGCTGTTGCCGAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 3563 255 36M * 0 0 GTCTATTATGGAAAACACCAATCTTTCCAAGCAACA * -HWI-EAS91_1_306UPAAXX 16 phiX174 4198 255 1H35M * 0 0 ATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 2907 255 1H35M * 0 0 CCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2843 255 35M1H * 0 0 TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTG * -HWI-EAS91_1_306UPAAXX 0 phiX174 4374 255 36M * 0 0 GTTACGCAGTTTTGCCGCAAGCTGGCTGCTGTACGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1940 255 1H35M * 0 0 ATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCC * -HWI-EAS91_1_306UPAAXX 16 phiX174 5073 255 36M * 0 0 GAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4434 255 36M * 0 0 TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG * -HWI-EAS91_1_306UPAAXX 16 phiX174 1274 255 1H35M * 0 0 GCCACTCCTCTCCCGACTGTTAACACTACTGGTTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4440 255 36M * 0 0 TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 3920 255 36M * 0 0 GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 4587 255 1H35M * 0 0 CTCACGTTGGCTGACGACCGATTAGAGGCGTTTTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1707 255 36M * 0 0 TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1582 255 36M * 0 0 GGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1588 255 36M * 0 0 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1180 255 34M2H * 0 0 TATTGACTCTACTGTAGACATTTTTACTTTTTAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 2698 255 36M * 0 0 ACCCTCCCGACTGCCTATGATGTTTATCCTTTGAAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3167 255 36M * 0 0 TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG * -HWI-EAS91_1_306UPAAXX 16 phiX174 2289 255 36M * 0 0 AGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 441 255 36M * 0 0 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1012 255 36M * 0 0 TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 161 255 36M * 0 0 GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1165 255 1H35M * 0 0 GCGTCGTGGCCTTGCTATTGACTCTACTGTAGACA * -HWI-EAS91_1_306UPAAXX 16 phiX174 5010 255 36M * 0 0 GTGGATAAATACGTTAACAAAAAGTCAGATATGGAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2286 255 36M * 0 0 AAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 801 255 36M * 0 0 GCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1587 255 36M * 0 0 TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG * -HWI-EAS91_1_306UPAAXX 0 phiX174 4578 255 36M * 0 0 TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG * -HWI-EAS91_1_306UPAAXX 16 phiX174 3570 255 36M * 0 0 ATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTT * -HWI-EAS91_1_306UPAAXX 16 phiX174 496 255 36M * 0 0 TTCTGACGAGTAACAAAGTTTGGATTGCTACTGACC * -HWI-EAS91_1_306UPAAXX 0 phiX174 441 255 36M * 0 0 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 4754 255 36M * 0 0 TAATGGCCGTCTTCATTTCCATGCGGTGCATTTTAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 4235 255 35M1H * 0 0 TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATG * -HWI-EAS91_1_306UPAAXX 16 phiX174 2293 255 36M * 0 0 TGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1579 255 1H35M * 0 0 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA * -HWI-EAS91_1_306UPAAXX 0 phiX174 3332 255 36M * 0 0 TGGTGCTGATGCTTCCTCTGCTGGTATGGTTTACGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4132 255 36M * 0 0 TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4775 255 36M * 0 0 TGCGGTGCATTTTATGCGGACACTTCCTACAGGTAG * -HWI-EAS91_1_306UPAAXX 16 phiX174 1 255 4H32M * 0 0 GAGTTTTATCGCTTCCATGACGCAGAAGTTAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 54 255 36M * 0 0 AGTCGAAAAATTATCTTGATAAAGCAGGAATTACTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 680 255 1H35M * 0 0 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 231 255 36M * 0 0 TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 4192 255 36M * 0 0 ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG * -HWI-EAS91_1_306UPAAXX 16 phiX174 1580 255 36M * 0 0 ATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACT * -HWI-EAS91_1_306UPAAXX 16 phiX174 611 255 36M * 0 0 TGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 4532 255 1H35M * 0 0 GATGAATGCAATGCGACAGGCTCATGCTGATGGTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 638 255 35M1H * 0 0 TTATTATGTTCATCCCGTCAACATTCAAACTGCCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1957 255 36M * 0 0 TTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 2728 255 36M * 0 0 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3979 255 36M * 0 0 TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG * -HWI-EAS91_1_306UPAAXX 16 phiX174 540 255 1H35M * 0 0 GCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 1852 255 36M * 0 0 GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4684 255 36M * 0 0 AGGGTCGCAAGGCTAATGATTCACACGCCGACTGCT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3690 255 36M * 0 0 AAGGTTAGTGCTGAGGTTGACTTAGTTCATCATCAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 584 255 36M * 0 0 TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT * -HWI-EAS91_1_306UPAAXX 16 phiX174 3852 255 1H35M * 0 0 ACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2857 255 35M1H * 0 0 TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1728 255 36M * 0 0 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC * -HWI-EAS91_1_306UPAAXX 0 phiX174 4658 255 35M1H * 0 0 TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1721 255 36M * 0 0 TCTAATCTCTGGGCATCTGGCTATGATGTTGATGGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1954 255 36M * 0 0 TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC * -HWI-EAS91_1_306UPAAXX 0 phiX174 962 255 36M * 0 0 TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 2175 255 36M * 0 0 ACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2442 255 36M * 0 0 TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 5126 255 1H35M * 0 0 CAGAATCAGAATGAGCCGCAACTTCGGGATGAAAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 5206 255 36M * 0 0 CCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2879 255 36M * 0 0 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 2004 255 36M * 0 0 TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG * -HWI-EAS91_1_306UPAAXX 0 phiX174 2154 255 36M * 0 0 TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 1093 255 35M1H * 0 0 TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCG * -HWI-EAS91_1_306UPAAXX 16 phiX174 3127 255 36M * 0 0 AGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 3544 255 36M * 0 0 AGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2508 255 35M1H * 0 0 ATCGTCAACGTTATATTTTGATAGTTTGACGTTTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 3600 255 36M * 0 0 CAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1734 255 36M * 0 0 CATCTGGCTATGATGTTGATGGAACTGACCAAACGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2655 255 33M3H * 0 0 TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 2934 255 36M * 0 0 TTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 1522 255 36M * 0 0 TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4063 255 36M * 0 0 AGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 3395 255 36M * 0 0 TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 409 255 36M * 0 0 AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1867 255 36M * 0 0 TGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 3780 255 36M * 0 0 TCTAATGTCGTCACTGATGCTGCTTCTGGTGTGTTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3982 255 36M * 0 0 TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG * -HWI-EAS91_1_306UPAAXX 16 phiX174 2815 255 36M * 0 0 TATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 1242 255 36M * 0 0 AGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCA * -HWI-EAS91_1_306UPAAXX 16 phiX174 4659 255 1H35M * 0 0 ATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2436 255 36M * 0 0 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC * -HWI-EAS91_1_306UPAAXX 0 phiX174 527 255 35M1H * 0 0 TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTT * -HWI-EAS91_1_306UPAAXX 16 phiX174 4817 255 36M * 0 0 CCCTAATTTGGGTCGTCGGGTACGCAATCGCCGCCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2100 255 36M * 0 0 AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG * -HWI-EAS91_1_306UPAAXX 16 phiX174 2762 255 36M * 0 0 TCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA * -HWI-EAS91_1_306UPAAXX 0 phiX174 4955 255 36M * 0 0 TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 3761 255 36M * 0 0 CGCTACTGCAAAGGATATTTCTAATGTCGTCACTGA * -HWI-EAS91_1_306UPAAXX 16 phiX174 4499 255 36M * 0 0 GAAATCGCGTAGAGGCTTTACTATTCAGCGTTTGAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 4039 255 36M * 0 0 TCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCA * -HWI-EAS91_1_306UPAAXX 16 phiX174 4788 255 36M * 0 0 ATGCGGACACTTCCTACAGGTAGCGTTGACCCTAAT * -HWI-EAS91_1_306UPAAXX 0 phiX174 1002 255 36M * 0 0 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4381 255 36M * 0 0 AGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 4256 255 36M * 0 0 GAAGTTAATGGATGAATTGGCACAATGCTACAATGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 10 255 1H35M * 0 0 CGCTTCCATGACGCAGAAGTTAACACTTTCGGATA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1579 255 36M * 0 0 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATAC * -HWI-EAS91_1_306UPAAXX 0 phiX174 3862 255 36M * 0 0 ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3278 255 36M * 0 0 TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT * -HWI-EAS91_1_306UPAAXX 0 phiX174 349 255 36M * 0 0 TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG * -HWI-EAS91_1_306UPAAXX 0 phiX174 4608 255 36M * 0 0 TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT * -HWI-EAS91_1_306UPAAXX 0 phiX174 3808 255 35M1H * 0 0 GTGTGGTTGATATTTTTCATGGTATTGATAAAGCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 4753 255 36M * 0 0 CTAATGGCCGTCTTCATTTCCATGCGGTGCATTTTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 286 255 36M * 0 0 GAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTT * -HWI-EAS91_1_306UPAAXX 0 phiX174 2850 255 36M * 0 0 TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT * -HWI-EAS91_1_306UPAAXX 16 phiX174 680 255 1H35M * 0 0 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 4740 255 36M * 0 0 GAGTATGGTACAGCTAATGGCCGTCTTCATTTCCAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 2467 255 1H35M * 0 0 CCAGCTTCTTCGGCACCTGTTTTACAGACACCTAA * -HWI-EAS91_1_306UPAAXX 0 phiX174 320 255 34M2H * 0 0 TTGACATTTTAAAAGAGCGTGGATTACTATCTGA * -HWI-EAS91_1_306UPAAXX 0 phiX174 1180 255 34M2H * 0 0 TATTGACTCTACTGTAGACATTTTTACTTTTTAT * -HWI-EAS91_1_306UPAAXX 16 phiX174 521 255 36M * 0 0 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGA * -HWI-EAS91_1_306UPAAXX 16 phiX174 333 255 36M * 0 0 AGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCA * -HWI-EAS91_1_306UPAAXX 0 phiX174 2756 255 36M * 0 0 ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC * -HWI-EAS91_1_306UPAAXX 16 phiX174 4957 255 36M * 0 0 ATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATA * -HWI-EAS91_1_306UPAAXX 16 phiX174 72 255 36M * 0 0 ATAAAGCAGGAATTACTACTGCTTGTTTACGAATTA * -HWI-EAS91_1_306UPAAXX 16 phiX174 4249 255 36M * 0 0 TTACTGAGAAGTTAATGGATGAATTGGCACAATGCT * -HWI-EAS91_1_306UPAAXX 16 phiX174 2592 255 36M * 0 0 CAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA * -HWI-EAS91_1_306UPAAXX 16 phiX174 1974 255 36M * 0 0 CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTT * -HWI-EAS91_1_306UPAAXX 16 phiX174 3540 255 36M * 0 0 AAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAA * -HWI-EAS91_1_306UPAAXX 16 phiX174 4130 255 36M * 0 0 CATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTAT *
--- a/test-data/lastz_wrapper_out3.tabular Tue Oct 13 12:23:37 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,22 +0,0 @@ -phiX174 1164 1165 + 5386 HWI-EAS91_1_306UPAAXX:6:1:112:1280 26 27 + 36 G T GTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTG GTTGGCGCTCTCCGTCTTTCTCCATTTCGTCGTG -phiX174 2490 2491 + 5386 HWI-EAS91_1_306UPAAXX:6:1:486:822 10 11 - 36 C A ACCTGTTTTACAGACACCTAAAGCTACATCGTCAAC ACCTGTTTTAAAGACACCTAAAGCTACATCGTCAAC -phiX174 2205 2206 + 5386 HWI-EAS91_1_306UPAAXX:6:1:217:737 14 15 + 36 G T GTTCAGTTGTTGCAGTGGAATA GTTCAGTTGTTGCATTGGAATA -phiX174 2173 2174 + 5386 HWI-EAS91_1_306UPAAXX:6:1:259:1389 26 27 + 36 G T GCGTACTTATTCGCCACCATGATTATGACCAGTGTT GCGTACTTATTCGCCACCATGATTATTACCAGTGTT -phiX174 578 579 + 5386 HWI-EAS91_1_306UPAAXX:6:1:317:1411 8 9 + 36 G T GTACGCTGGACTTTGTGGGATACCCTCGCTTTCCT GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCT -phiX174 2992 2993 + 5386 HWI-EAS91_1_306UPAAXX:6:1:458:1629 23 24 + 36 G T GCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGT GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT -phiX174 1995 1996 + 5386 HWI-EAS91_1_306UPAAXX:6:1:590:331 14 15 + 36 G T GAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGAT GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT -phiX174 3261 3262 + 5386 HWI-EAS91_1_306UPAAXX:6:1:221:1238 17 18 - 36 C A AAGTCTGCCGCTGATAAAGGAAAGGATAC AAGTCTGCCGATGATAAAGGAAAGGATAC -phiX174 4109 4110 + 5386 HWI-EAS91_1_306UPAAXX:6:1:478:252 12 13 - 36 C A ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC ATGCTTGGCTTCAATAAGCAGATGGATAACCGCATC -phiX174 3126 3127 + 5386 HWI-EAS91_1_306UPAAXX:6:1:589:96 28 29 - 36 A G CAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCC CAAGGCTCTAATGTTCCTAACCCTGATGGGGCCGCC -phiX174 2569 2570 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1472:716 23 24 + 36 G T TGGTAATGGTGGTTTTCTTCATTGCATTCAG TGGTAATGGTGGTTTTCTTCATTTCATTCAG -phiX174 2972 2973 + 5386 HWI-EAS91_1_306UPAAXX:6:1:836:1204 17 18 + 36 G T TTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAAT TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT -phiX174 2234 2235 + 5386 HWI-EAS91_1_306UPAAXX:6:1:172:1412 13 14 - 36 C A AAATTTAATGTGACCGTTTATCGCAATCTGCCGACC AAATTTAATGTGAACGTTTATCGCAATCTGCCGACC -phiX174 2902 2903 + 5386 HWI-EAS91_1_306UPAAXX:6:1:540:1415 18 19 + 36 G T TTATTAAAGAGATTATTTGTCTCCAGCCACTTA TTATTAAAGAGATTATTTTTCTCCAGCCACTTA -phiX174 2837 2838 + 5386 HWI-EAS91_1_306UPAAXX:6:1:253:1162 14 15 - 36 T A ATGGTTTGGTCTAACTTTACCGCTACTAAATGC ATGGTTTGGTCAAACTTTACCGCTACTAAATGC -phiX174 4662 4663 + 5386 HWI-EAS91_1_306UPAAXX:6:1:828:754 26 27 + 36 G T TTTGCGTGACTATTTTCGTGATATTGGTCGTATGGT TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT -phiX174 4202 4203 + 5386 HWI-EAS91_1_306UPAAXX:6:1:891:1437 19 20 + 36 G T TAATGGTGATATGTATGTTGACGGCCATAAGGCTG TAATGGTGATATGTATGTTTACGTCCATAAGGCTG -phiX174 4206 4207 + 5386 HWI-EAS91_1_306UPAAXX:6:1:891:1437 23 24 + 36 G T TAATGGTGATATGTATGTTGACGGCCATAAGGCTG TAATGGTGATATGTATGTTTACGTCCATAAGGCTG -phiX174 430 431 + 5386 HWI-EAS91_1_306UPAAXX:6:1:203:667 9 10 - 36 T C AGACCGCTTTGGCCTCTATTAAGC AGACCGCCTTGGCCTCTATTAAGC -phiX174 2857 2858 + 5386 HWI-EAS91_1_306UPAAXX:6:1:578:971 15 16 + 36 G T CGCTACTAAATGCCGCGGATTGGTTTCG CGCTACTAAATTCCGCGGATTGGTTTCG -phiX174 3112 3113 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1721:1800 27 28 - 36 T C TCAAGGCTCTAATGTTCCTAACCC TCAAGGCTCTAATGTCCCTAACCC -phiX174 4825 4826 + 5386 HWI-EAS91_1_306UPAAXX:6:1:815:1640 9 10 - 36 T G CCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCA CCCTAATTTGGGTCGTCGGGTACGCAATCGCCGCCA
--- a/test-data/lastz_wrapper_out4.tabular Tue Oct 13 12:23:37 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,491 +0,0 @@ -14 phiX1 + 2310 2192 2191 2213 22 GTTCAGTTGTTGCAGTGGAATA HWI-EAS91_1_306UPAAXX + 36 1 0 22 1 0 22 22 GTTCAGTTGTTGCATTGGAATA ..............x....... 22M 21/22 95.5% 22/36 61.1% 0/22 0.0% 2191 NA -16 phiX1 + 2310 424 423 447 24 AGACCGCTTTGGCCTCTATTAAGC HWI-EAS91_1_306UPAAXX - 36 3 2 26 11 10 34 24 AGACCGCCTTGGCCTCTATTAAGC .......:................ 24M 23/24 95.8% 24/36 66.7% 0/24 0.0% 421 NA -16 phiX2 + 3076 788 787 811 24 TCAAGGCTCTAATGTTCCTAACCC HWI-EAS91_1_306UPAAXX - 36 13 12 36 1 0 24 24 TCAAGGCTCTAATGTCCCTAACCC ...............:........ 24M 23/24 95.8% 24/36 66.7% 0/24 0.0% 775 NA -18 phiX1 + 2310 657 656 674 18 AACATTCAAACGGCCTGT HWI-EAS91_1_306UPAAXX - 36 19 18 36 1 0 18 18 AACATTCAAACGGCCTGT .................. 18M 18/18 100.0% 18/36 50.0% 0/18 0.0% 638 NA -18 phiX2 + 3076 1421 1420 1438 18 GAATCAGCGGTATGGCTC HWI-EAS91_1_306UPAAXX + 36 1 0 18 1 0 18 18 GAATCAGCGGTATGGCTC .................. 18M 18/18 100.0% 18/36 50.0% 0/18 0.0% 1420 NA -18 phiX2 + 3076 2625 2624 2642 18 TCTGGTTGGTTGTGGCCT HWI-EAS91_1_306UPAAXX + 36 1 0 18 1 0 18 18 TCTGGTTGGTTGTGGCCT .................. 18M 18/18 100.0% 18/36 50.0% 0/18 0.0% 2624 NA -19 phiX2 + 3076 1874 1873 1908 35 TAATGGTGATATGTATGTTGACGGCCATAAGGCTG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TAATGGTGATATGTATGTTTACGTCCATAAGGCTG ...................x...x........... 35M 33/35 94.3% 35/36 97.2% 0/35 0.0% 1873 NA -20 phiX2 + 3076 537 536 564 28 CGCTACTAAATGCCGCGGATTGGTTTCG HWI-EAS91_1_306UPAAXX + 36 5 4 32 5 4 32 28 CGCTACTAAATTCCGCGGATTGGTTTCG ...........x................ 28M 27/28 96.4% 28/36 77.8% 0/28 0.0% 532 NA -20 phiX2 + 3076 628 627 647 20 GTGCTATTGCTGGCGGTATT HWI-EAS91_1_306UPAAXX + 36 1 0 20 1 0 20 20 GTGCTATTGCTGGCGGTATT .................... 20M 20/20 100.0% 20/36 55.6% 0/20 0.0% 627 NA -21 phiX1 + 2310 1 0 21 21 GAGTTTTATCGCTTCCATGAC HWI-EAS91_1_306UPAAXX - 36 16 15 36 1 0 21 21 GAGTTTTATCGCTTCCATGAC ..................... 21M 21/21 100.0% 21/36 58.3% 0/21 0.0% -15 -21 -21 phiX2 + 3076 1990 1989 2010 21 AACTTGATATTAATAACACTA HWI-EAS91_1_306UPAAXX - 36 16 15 36 1 0 21 21 AACTTGATATTAATAACACTA ..................... 21M 21/21 100.0% 21/36 58.3% 0/21 0.0% 1974 NA -21 phiX2 + 3076 942 941 970 29 AAGTCTGCCGCTGATAAAGGAAAGGATAC HWI-EAS91_1_306UPAAXX - 36 8 7 36 1 0 29 29 AAGTCTGCCGATGATAAAGGAAAGGATAC ..........x.................. 29M 28/29 96.6% 29/36 80.6% 0/29 0.0% 934 NA -22 phiX1 + 2310 2289 2288 2310 22 AGATTGAGTGTGAGGTTATAAC HWI-EAS91_1_306UPAAXX - 36 1 0 22 15 14 36 22 AGATTGAGTGTGAGGTTATAAC ...................... 22M 22/22 100.0% 22/36 61.1% 0/22 0.0% 2288 22 -23 phiX2 + 3076 237 236 267 31 TGGTAATGGTGGTTTTCTTCATTGCATTCAG HWI-EAS91_1_306UPAAXX + 36 1 0 31 1 0 31 31 TGGTAATGGTGGTTTTCTTCATTTCATTCAG .......................x....... 31M 30/31 96.8% 31/36 86.1% 0/31 0.0% 236 NA -24 phiX2 + 3076 1420 1419 1443 24 AGAATCAGCGGTATGGCTCTTCTC HWI-EAS91_1_306UPAAXX + 36 1 0 24 1 0 24 24 AGAATCAGCGGTATGGCTCTTCTC ........................ 24M 24/24 100.0% 24/36 66.7% 0/24 0.0% 1419 NA -25 phiX1 + 2310 2286 2285 2310 25 AAAAGATTGAGTGTGAGGTTATAAC HWI-EAS91_1_306UPAAXX - 36 1 0 25 12 11 36 25 AAAAGATTGAGTGTGAGGTTATAAC ......................... 25M 25/25 100.0% 25/36 69.4% 0/25 0.0% 2285 25 -25 phiX2 + 3076 188 187 212 25 CTAAAGCTACATCGTCAACGTTATA HWI-EAS91_1_306UPAAXX - 36 12 11 36 1 0 25 25 CTAAAGCTACATCGTCAACGTTATA ......................... 25M 25/25 100.0% 25/36 69.4% 0/25 0.0% 176 NA -25 phiX2 + 3076 2345 2344 2369 25 TGATATTGGTCGTATGGTTCTTGCT HWI-EAS91_1_306UPAAXX + 36 1 0 25 1 0 25 25 TGATATTGGTCGTATGGTTCTTGCT ......................... 25M 25/25 100.0% 25/36 69.4% 0/25 0.0% 2344 NA -25 phiX2 + 3076 517 516 549 33 ATGGTTTGGTCTAACTTTACCGCTACTAAATGC HWI-EAS91_1_306UPAAXX - 36 4 3 36 1 0 33 33 ATGGTTTGGTCAAACTTTACCGCTACTAAATGC ...........x..................... 33M 32/33 97.0% 33/36 91.7% 0/33 0.0% 513 NA -25 phiX2 + 3076 575 574 607 33 TTATTAAAGAGATTATTTGTCTCCAGCCACTTA HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 TTATTAAAGAGATTATTTTTCTCCAGCCACTTA ..................x.............. 33M 32/33 97.0% 33/36 91.7% 0/33 0.0% 574 NA -26 phiX1 + 2310 1139 1138 1172 34 GTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTG HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GTTGGCGCTCTCCGTCTTTCTCCATTTCGTCGTG ..........................x....... 34M 33/34 97.1% 34/36 94.4% 0/34 0.0% 1138 NA -26 phiX1 + 2310 664 663 689 26 AAACGGCCTGTCTCATCATGGAAGGC HWI-EAS91_1_306UPAAXX - 36 11 10 36 1 0 26 26 AAACGGCCTGTCTCATCATGGAAGGC .......................... 26M 26/26 100.0% 26/36 72.2% 0/26 0.0% 653 NA -26 phiX1 + 2310 664 663 689 26 AAACGGCCTGTCTCATCATGGAAGGC HWI-EAS91_1_306UPAAXX - 36 11 10 36 1 0 26 26 AAACGGCCTGTCTCATCATGGAAGGC .......................... 26M 26/26 100.0% 26/36 72.2% 0/26 0.0% 653 NA -26 phiX2 + 3076 1472 1471 1497 26 TAATGTCGTCACTGATGCTGCTTCTG HWI-EAS91_1_306UPAAXX + 36 1 0 26 1 0 26 26 TAATGTCGTCACTGATGCTGCTTCTG .......................... 26M 26/26 100.0% 26/36 72.2% 0/26 0.0% 1471 NA -26 phiX2 + 3076 1726 1725 1751 26 GTATCGAGGCTCTTAAACCTGCTATT HWI-EAS91_1_306UPAAXX + 36 1 0 26 1 0 26 26 GTATCGAGGCTCTTAAACCTGCTATT .......................... 26M 26/26 100.0% 26/36 72.2% 0/26 0.0% 1725 NA -27 phiX1 + 2310 558 557 584 27 GCTTGCGTTTATGGTACGCTGGACTTT HWI-EAS91_1_306UPAAXX + 36 1 0 27 1 0 27 27 GCTTGCGTTTATGGTACGCTGGACTTT ........................... 27M 27/27 100.0% 27/36 75.0% 0/27 0.0% 557 NA -27 phiX1 + 2310 571 570 605 35 GTACGCTGGACTTTGTGGGATACCCTCGCTTTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCT ........x.......................... 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 570 NA -27 phiX2 + 3076 1911 1910 1937 27 TCTGACGTTCGTGATGAGTTTGTATCT HWI-EAS91_1_306UPAAXX + 36 1 0 27 1 0 27 27 TCTGACGTTCGTGATGAGTTTGTATCT ........................... 27M 27/27 100.0% 27/36 75.0% 0/27 0.0% 1910 NA -27 phiX2 + 3076 2346 2345 2372 27 GATATTGGTCGTATGGTTCTTGCTGCC HWI-EAS91_1_306UPAAXX + 36 1 0 27 1 0 27 27 GATATTGGTCGTATGGTTCTTGCTGCC ........................... 27M 27/27 100.0% 27/36 75.0% 0/27 0.0% 2345 NA -27 phiX2 + 3076 2362 2361 2388 27 TTCTTGCTGCCGAGGGTCGCAAGGCTA HWI-EAS91_1_306UPAAXX + 36 1 0 27 1 0 27 27 TTCTTGCTGCCGAGGGTCGCAAGGCTA ........................... 27M 27/27 100.0% 27/36 75.0% 0/27 0.0% 2361 NA -27 phiX2 + 3076 2457 2456 2491 35 CATTTCCATGCGGTGCACTTTATGCGGACACTTCC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CATTTCCATGCGGTGCATTTTATGCGGACACTTCC .................:................. 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 2455 NA -28 phiX1 + 2310 1626 1625 1661 36 GTGATTACTTCATGCAGCGTTACCATGATGTTATTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTGATTACTTCATGCAGCGTTACCGTGATGTTATTT ........................:........... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1625 NA -28 phiX1 + 2310 1982 1981 2017 36 GAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT ..............x..................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1981 NA -28 phiX1 + 2310 2148 2147 2183 36 GCGTACTTATTCGCCACCATGATTATGACCAGTGTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGTACTTATTCGCCACCATGATTATTACCAGTGTT ..........................x......... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2147 NA -28 phiX1 + 2310 2222 2221 2257 36 AAATTTAATGTGACCGTTTATCGCAATCTGCCGACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAATTTAATGTGAACGTTTATCGCAATCTGCCGACC .............x...................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2221 NA -28 phiX2 + 3076 171 170 206 36 ACCTGTTTTACAGACACCTAAAGCTACATCGTCAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCTGTTTTAAAGACACCTAAAGCTACATCGTCAAC ..........x......................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 170 NA -28 phiX2 + 3076 1788 1787 1823 36 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTTGGCTTCAATAAGCAGATGGATAACCGCATC ............x....................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1787 NA -28 phiX2 + 3076 1918 1917 1945 28 TTCGTGATGAGTTTGTATCTGTTACTGA HWI-EAS91_1_306UPAAXX + 36 1 0 28 1 0 28 28 TTCGTGATGAGTTTGTATCTGTTACTGA ............................ 28M 28/28 100.0% 28/36 77.8% 0/28 0.0% 1917 NA -28 phiX2 + 3076 2189 2188 2224 36 GAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAAATCGCGTAGAGGCTTTACTATTCAGCGTTTGAT ...................:................ 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2188 NA -28 phiX2 + 3076 2325 2324 2352 28 GCTTTGCGTGACTATTTTCGTGATATTG HWI-EAS91_1_306UPAAXX + 36 1 0 28 1 0 28 28 GCTTTGCGTGACTATTTTCGTGATATTG ............................ 28M 28/28 100.0% 28/36 77.8% 0/28 0.0% 2324 NA -28 phiX2 + 3076 2327 2326 2362 36 TTTGCGTGACTATTTTCGTGATATTGGTCGTATGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT ..........................x......... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2326 NA -28 phiX2 + 3076 2465 2464 2500 36 TGCGGTGCACTTTATGCGGACACTTCCTACAGGTAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCGGTGCATTTTATGCGGACACTTCCTACAGGTAG .........:.......................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2464 NA -28 phiX2 + 3076 2507 2506 2542 36 CCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCCTAATTTGGGTCGTCGGGTACGCAATCGCCGCCA .........x.......................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2506 NA -28 phiX2 + 3076 646 645 681 36 TTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT .................x.................. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 645 NA -28 phiX2 + 3076 660 659 695 36 GCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT .......................x............ 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 659 NA -28 phiX2 + 3076 789 788 824 36 CAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAGGCTCTAATGTTCCTAACCCTGATGGGGCCGCC ............................:....... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 788 NA -29 phiX1 + 2310 598 597 626 29 GCTTTCCTGCTCCTGTTGAGTTTATTGCT HWI-EAS91_1_306UPAAXX + 36 1 0 29 1 0 29 29 GCTTTCCTGCTCCTGTTGAGTTTATTGCT ............................. 29M 29/29 100.0% 29/36 80.6% 0/29 0.0% 597 NA -29 phiX2 + 3076 1176 1175 1204 29 CAGAATACGAAAGACCAGGTATATGCACA HWI-EAS91_1_306UPAAXX - 36 8 7 36 1 0 29 29 CAGAATACGAAAGACCAGGTATATGCACA ............................. 29M 29/29 100.0% 29/36 80.6% 0/29 0.0% 1168 NA -29 phiX2 + 3076 1518 1517 1546 29 GGTATTGATAAAGCTGTTGCCGATACTTG HWI-EAS91_1_306UPAAXX + 36 1 0 29 1 0 29 29 GGTATTGATAAAGCTGTTGCCGATACTTG ............................. 29M 29/29 100.0% 29/36 80.6% 0/29 0.0% 1517 NA -29 phiX2 + 3076 2282 2281 2310 29 GTTGGCTGACGACCGATTAGAGGCGTTTT HWI-EAS91_1_306UPAAXX + 36 1 0 29 1 0 29 29 GTTGGCTGACGACCGATTAGAGGCGTTTT ............................. 29M 29/29 100.0% 29/36 80.6% 0/29 0.0% 2281 NA -29 phiX2 + 3076 3048 3047 3076 29 ATAAAAATGATTGGCGTATCCAACCTGCA HWI-EAS91_1_306UPAAXX + 36 1 0 29 1 0 29 29 ATAAAAATGATTGGCGTATCCAACCTGCA ............................. 29M 29/29 100.0% 29/36 80.6% 0/29 0.0% 3047 29 -29 phiX2 + 3076 953 952 981 29 TGATAAAGGAAAGGATACTCGTGATTATC HWI-EAS91_1_306UPAAXX - 36 8 7 36 1 0 29 29 TGATAAAGGAAAGGATACTCGTGATTATC ............................. 29M 29/29 100.0% 29/36 80.6% 0/29 0.0% 945 NA -30 phiX1 + 2310 1112 1111 1141 30 GCTGGCGACTCCTTCGAGATGGACGCCGTT HWI-EAS91_1_306UPAAXX + 36 1 0 30 1 0 30 30 GCTGGCGACTCCTTCGAGATGGACGCCGTT .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 1111 NA -30 phiX1 + 2310 1702 1701 1731 30 TCCTTTACTTGTCATGCGCTCTAATCTCTG HWI-EAS91_1_306UPAAXX + 36 1 0 30 1 0 30 30 TCCTTTACTTGTCATGCGCTCTAATCTCTG .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 1701 NA -30 phiX1 + 2310 624 623 653 30 GCTGCCGTCATTGCTTATTATGTTCATCCC HWI-EAS91_1_306UPAAXX + 36 1 0 30 1 0 30 30 GCTGCCGTCATTGCTTATTATGTTCATCCC .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 623 NA -30 phiX1 + 2310 638 637 667 30 TTATTATGTTCATCCCGTCAACATTCAAAC HWI-EAS91_1_306UPAAXX + 36 1 0 30 1 0 30 30 TTATTATGTTCATCCCGTCAACATTCAAAC .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 637 NA -30 phiX1 + 2310 81 80 110 30 GAATTACTACTGCTTGTTTACGAATTAAAT HWI-EAS91_1_306UPAAXX + 36 1 0 30 1 0 30 30 GAATTACTACTGCTTGTTTACGAATTAAAT .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 80 NA -30 phiX1 + 2310 989 988 1018 30 TGAGGATAAATTATGTCTAATATTCAAACT HWI-EAS91_1_306UPAAXX + 36 1 0 30 1 0 30 30 TGAGGATAAATTATGTCTAATATTCAAACT .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 988 NA -30 phiX2 + 3076 1468 1467 1497 30 TTTCTAATGTCGTCACTGATGCTGCTTCTG HWI-EAS91_1_306UPAAXX + 36 1 0 30 1 0 30 30 TTTCTAATGTCGTCACTGATGCTGCTTCTG .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 1467 NA -30 phiX2 + 3076 1500 1499 1529 30 GTGGTTGATATTTTTCATGGTATTGATAAA HWI-EAS91_1_306UPAAXX + 36 1 0 30 1 0 30 30 GTGGTTGATATTTTTCATGGTATTGATAAA .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 1499 NA -30 phiX2 + 3076 1863 1862 1892 30 GTTGAGTTCGATAATGGTGATATGTATGTT HWI-EAS91_1_306UPAAXX + 36 1 0 30 1 0 30 30 GTTGAGTTCGATAATGGTGATATGTATGTT .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 1862 NA -30 phiX2 + 3076 2020 2019 2055 36 GCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCCCGAAGGGGACNANAAATGGTTTTTAGAGAACG ..............X.X................... 36M 34/34 100.0% 36/36 100.0% 0/36 0.0% 2019 NA -30 phiX2 + 3076 2444 2443 2473 30 TAATGGCCGTCTTCATTTCCATGCGGTGCA HWI-EAS91_1_306UPAAXX - 36 1 0 30 7 6 36 30 TAATGGCCGTCTTCATTTCCATGCGGTGCA .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 2443 NA -30 phiX2 + 3076 502 501 531 30 GTTTATGTTGGTTTCATGGTTTGGTCTAAC HWI-EAS91_1_306UPAAXX - 36 7 6 36 1 0 30 30 GTTTATGTTGGTTTCATGGTTTGGTCTAAC .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 495 NA -30 phiX2 + 3076 858 857 887 30 GGTAAAGGACTTCTTGAAGGTACGTTGCAG HWI-EAS91_1_306UPAAXX + 36 1 0 30 1 0 30 30 GGTAAAGGACTTCTTGAAGGTACGTTGCAG .............................. 30M 30/30 100.0% 30/36 83.3% 0/30 0.0% 857 NA -31 phiX1 + 2310 1346 1345 1376 31 GATACCAATAAAATCCCTAAGCATTTGTTTC HWI-EAS91_1_306UPAAXX + 36 1 0 31 1 0 31 31 GATACCAATAAAATCCCTAAGCATTTGTTTC ............................... 31M 31/31 100.0% 31/36 86.1% 0/31 0.0% 1345 NA -31 phiX1 + 2310 1807 1806 1837 31 TAAACATTCTGTGCCGCGTTTCTTTGTTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 31 1 0 31 31 TAAACATTCTGTGCCGCGTTTCTTTGTTCCT ............................... 31M 31/31 100.0% 31/36 86.1% 0/31 0.0% 1806 NA -31 phiX2 + 3076 1022 1021 1052 31 TGGTGCTGATGCTTCCTCTGCTGGTATGGTT HWI-EAS91_1_306UPAAXX + 36 1 0 31 1 0 31 31 TGGTGCTGATGCTTCCTCTGCTGGTATGGTT ............................... 31M 31/31 100.0% 31/36 86.1% 0/31 0.0% 1021 NA -31 phiX2 + 3076 198 197 228 31 ATCGTCAACGTTATATTTTGATAGTTTGACG HWI-EAS91_1_306UPAAXX + 36 1 0 31 1 0 31 31 ATCGTCAACGTTATATTTTGATAGTTTGACG ............................... 31M 31/31 100.0% 31/36 86.1% 0/31 0.0% 197 NA -31 phiX2 + 3076 2064 2063 2094 31 GTTACGCAGTTTTGCCGCAAGCTGGCTGCTG HWI-EAS91_1_306UPAAXX + 36 1 0 31 1 0 31 31 GTTACGCAGTTTTGCCGCAAGCTGGCTGCTG ............................... 31M 31/31 100.0% 31/36 86.1% 0/31 0.0% 2063 NA -31 phiX2 + 3076 2443 2442 2473 31 CTAATGGCCGTCTTCATTTCCATGCGGTGCA HWI-EAS91_1_306UPAAXX - 36 1 0 31 6 5 36 31 CTAATGGCCGTCTTCATTTCCATGCGGTGCA ............................... 31M 31/31 100.0% 31/36 86.1% 0/31 0.0% 2442 NA -31 phiX2 + 3076 2705 2704 2735 31 TAAATACGTTAACAAAAAGTCAGATATGGAC HWI-EAS91_1_306UPAAXX - 36 6 5 36 1 0 31 31 TAAATACGTTAACAAAAAGTCAGATATGGAC ............................... 31M 31/31 100.0% 31/36 86.1% 0/31 0.0% 2699 NA -31 phiX2 + 3076 331 330 361 31 GCCGACCCTAAATTTTTTGCCTGTTTGGTTC HWI-EAS91_1_306UPAAXX + 36 1 0 31 1 0 31 31 GCCGACCCTAAATTTTTTGCCTGTTTGGTTC ............................... 31M 31/31 100.0% 31/36 86.1% 0/31 0.0% 330 NA -32 phiX1 + 2310 1 0 32 32 GAGTTTTATCGCTTCCATGACGCAGAAGTTAA HWI-EAS91_1_306UPAAXX - 36 5 4 36 1 0 32 32 GAGTTTTATCGCTTCCATGACGCAGAAGTTAA ................................ 32M 32/32 100.0% 32/36 88.9% 0/32 0.0% -4 -32 -32 phiX1 + 2310 1266 1265 1297 32 GTGTTAATGCCACTCCTCTCCCGACTGTTAAC HWI-EAS91_1_306UPAAXX + 36 1 0 32 1 0 32 32 GTGTTAATGCCACTCCTCTCCCGACTGTTAAC ................................ 32M 32/32 100.0% 32/36 88.9% 0/32 0.0% 1265 NA -32 phiX1 + 2310 801 800 832 32 GCAGAAGAAAACGTGCGTCAAAAATTACGTGC HWI-EAS91_1_306UPAAXX - 36 1 0 32 5 4 36 32 GCAGAAGAAAACGTGCGTCAAAAATTACGTGC ................................ 32M 32/32 100.0% 32/36 88.9% 0/32 0.0% 800 NA -32 phiX2 + 3076 1380 1379 1411 32 AAGGTTAGTGCTGAGGTTGACTTAGTTCATCA HWI-EAS91_1_306UPAAXX + 36 1 0 32 1 0 32 32 AAGGTTAGTGCTGAGGTTGACTTAGTTCATCA ................................ 32M 32/32 100.0% 32/36 88.9% 0/32 0.0% 1379 NA -32 phiX2 + 3076 2145 2144 2176 32 GGTATTAAGGATGAGTGTTCAAGATTGCTGGA HWI-EAS91_1_306UPAAXX + 36 1 0 32 1 0 32 32 GGTATTAAGGATGAGTGTTCAAGATTGCTGGA ................................ 32M 32/32 100.0% 32/36 88.9% 0/32 0.0% 2144 NA -32 phiX2 + 3076 352 351 383 32 TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCC HWI-EAS91_1_306UPAAXX + 36 1 0 32 1 0 32 32 TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCC ................................ 32M 32/32 100.0% 32/36 88.9% 0/32 0.0% 351 NA -33 phiX1 + 2310 1203 1202 1235 33 TTACTTTTTATGTCCCTCATCGTCACGTTTATG HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 TTACTTTTTATGTCCCTCATCGTCACGTTTATG ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 1202 NA -33 phiX1 + 2310 1313 1312 1345 33 GACCATGCCGCTTTTCTTGGCACGATTAACCCT HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GACCATGCCGCTTTTCTTGGCACGATTAACCCT ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 1312 NA -33 phiX1 + 2310 1379 1378 1411 33 GGTTATTTGAATATCTATAACAACTATTTTAAA HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GGTTATTTGAATATCTATAACAACTATTTTAAA ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 1378 NA -33 phiX1 + 2310 1544 1543 1576 33 CGCCAAATGACGACTTCTACCACATCTATTGAC HWI-EAS91_1_306UPAAXX - 36 4 3 36 1 0 33 33 CGCCAAATGACGACTTCTACCACATCTATTGAC ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 1540 NA -33 phiX1 + 2310 636 635 668 33 GCTTATTATGTTCATCCCGTCAACATTCAAACG HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GCTTATTATGTTCATCCCGTCAACATTCAAACG ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 635 NA -33 phiX2 + 3076 1470 1469 1502 33 TCTAATGTCGTCACTGATGCTGCTTCTGGTGTG HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 TCTAATGTCGTCACTGATGCTGCTTCTGGTGTG ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 1469 NA -33 phiX2 + 3076 1662 1661 1694 33 GGCTTTTTTATGGTTCGTTCTTATTACCCTTCT HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GGCTTTTTTATGGTTCGTTCTTATTACCCTTCT ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 1661 NA -33 phiX2 + 3076 1864 1863 1896 33 TTGAGTTCGATAATGGTGATATGTATGTTGACG HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 TTGAGTTCGATAATGGTGATATGTATGTTGACG ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 1863 NA -33 phiX2 + 3076 2253 2252 2285 33 GGTTGGTTTATCGTTTTTGACACTCTCACGTTG HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GGTTGGTTTATCGTTTTTGACACTCTCACGTTG ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 2252 NA -33 phiX2 + 3076 2606 2605 2638 33 GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTG HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTG ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 2605 NA -33 phiX2 + 3076 345 344 377 33 TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTC ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 344 NA -33 phiX2 + 3076 428 427 460 33 GCCATGATGGTGGTTATTATACCGTCAAGGACT HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GCCATGATGGTGGTTATTATACCGTCAAGGACT ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 427 NA -33 phiX2 + 3076 572 571 604 33 AGGTTATTAAAGAGATTATTTGTCTCCAGCCAC HWI-EAS91_1_306UPAAXX - 36 4 3 36 1 0 33 33 AGGTTATTAAAGAGATTATTTGTCTCCAGCCAC ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 568 NA -33 phiX2 + 3076 884 883 916 33 GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTT HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTT ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 883 NA -34 phiX1 + 2310 1180 1179 1213 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 1179 NA -34 phiX1 + 2310 1180 1179 1213 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 1179 NA -34 phiX1 + 2310 1254 1253 1287 34 TCATGAAGGATGGTGTTAATGCCACTCCTCTCCC HWI-EAS91_1_306UPAAXX - 36 3 2 36 1 0 34 34 TCATGAAGGATGGTGTTAATGCCACTCCTCTCCC .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 1251 NA -34 phiX1 + 2310 320 319 353 34 TTGACATTTTAAAAGAGCGTGGATTACTATCTGA HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TTGACATTTTAAAAGAGCGTGGATTACTATCTGA .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 319 NA -34 phiX1 + 2310 582 581 615 34 TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTG HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTG .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 581 NA -34 phiX2 + 3076 1826 1825 1859 34 GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 1825 NA -34 phiX2 + 3076 201 200 234 34 GTCAACGTTATATTTTGATAGTTTGACGGTTAAT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GTCAACGTTATATTTTGATAGTTTGACGGTTAAT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 200 NA -34 phiX2 + 3076 2034 2033 2067 34 GAAAAATGGTTTTTAGAGAACGAGAAGACGGTTA HWI-EAS91_1_306UPAAXX - 36 3 2 36 1 0 34 34 GAAAAATGGTTTTTAGAGAACGAGAAGACGGTTA .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 2031 NA -34 phiX2 + 3076 2174 2173 2207 34 GGAGGCCTCCACTATGAAATCGCGTAGAGGCTTT HWI-EAS91_1_306UPAAXX - 36 1 0 34 3 2 36 34 GGAGGCCTCCACTATGAAATCGCGTAGAGGCTTT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 2173 NA -34 phiX2 + 3076 2296 2295 2329 34 GATTAGAGGCGTTTTATGATAATCCCAATGCTTT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GATTAGAGGCGTTTTATGATAATCCCAATGCTTT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 2295 NA -34 phiX2 + 3076 2475 2474 2508 34 TTTATGCGGACACTTCCTACAGGTAGCGTTGACC HWI-EAS91_1_306UPAAXX - 36 3 2 36 1 0 34 34 TTTATGCGGACACTTCCTACAGGTAGCGTTGACC .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 2472 NA -35 phiX1 + 2310 10 9 44 35 CGCTTCCATGACGCAGAAGTTAACACTTTCGGATA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CGCTTCCATGACGCAGAAGTTAACACTTTCGGATA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 8 NA -35 phiX1 + 2310 1045 1044 1079 35 TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1044 NA -35 phiX1 + 2310 1067 1066 1101 35 GGTCAGATTGGTCGTCTTATTACCATTTCAACTAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GGTCAGATTGGTCGTCTTATTACCATTTCAACTAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1065 NA -35 phiX1 + 2310 1093 1092 1127 35 TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1092 NA -35 phiX1 + 2310 1165 1164 1199 35 GCGTCGTGGCCTTGCTATTGACTCTACTGTAGACA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCGTCGTGGCCTTGCTATTGACTCTACTGTAGACA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1163 NA -35 phiX1 + 2310 1172 1171 1206 35 GGCCTTGCTATTGACTCTACTGTAGACATTTTTAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GGCCTTGCTATTGACTCTACTGTAGACATTTTTAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1170 NA -35 phiX1 + 2310 1249 1248 1283 35 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1247 NA -35 phiX1 + 2310 1274 1273 1308 35 GCCACTCCTCTCCCGACTGTTAACACTACTGGTTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCCACTCCTCTCCCGACTGTTAACACTACTGGTTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1272 NA -35 phiX1 + 2310 1294 1293 1328 35 TAACACTACTGGTTATATTGACCATGCCGCTTTTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TAACACTACTGGTTATATTGACCATGCCGCTTTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1292 NA -35 phiX1 + 2310 1508 1507 1542 35 TGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1506 NA -35 phiX1 + 2310 1521 1520 1555 35 TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1520 NA -35 phiX1 + 2310 1552 1551 1586 35 GACGACTTCTACCACATCTATTGACATTATGGGTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GACGACTTCTACCACATCTATTGACATTATGGGTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1550 NA -35 phiX1 + 2310 1579 1578 1613 35 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1577 NA -35 phiX1 + 2310 1613 1612 1647 35 ACTGACCAAGAACGTGATTACTTCATGCAGCGTTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ACTGACCAAGAACGTGATTACTTCATGCAGCGTTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1611 NA -35 phiX1 + 2310 1877 1876 1911 35 CCGCCTACTGCGACTAAAGAGATTCAGTACCTTAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCGCCTACTGCGACTAAAGAGATTCAGTACCTTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1875 NA -35 phiX1 + 2310 1940 1939 1974 35 ATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1938 NA -35 phiX1 + 2310 195 194 229 35 TCAACTAACGATTCTGTCAAAAACTGACGCGTTGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TCAACTAACGATTCTGTCAAAAACTGACGCGTTGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 194 NA -35 phiX1 + 2310 204 203 238 35 GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 203 NA -35 phiX1 + 2310 2112 2111 2146 35 TCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2110 NA -35 phiX1 + 2310 2212 2211 2246 35 TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2211 NA -35 phiX1 + 2310 2254 2253 2288 35 GACCACTCGCGATTCAATCATGACTTCGTGATAAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GACCACTCGCGATTCAATCATGACTTCGTGATAAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2253 NA -35 phiX1 + 2310 358 357 392 35 GATGCTGTTCAACCACTAATAGGTAAGAAATCATG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GATGCTGTTCAACCACTAATAGGTAAGAAATCATG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 357 NA -35 phiX1 + 2310 411 410 445 35 TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 410 NA -35 phiX1 + 2310 521 520 555 35 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 520 NA -35 phiX1 + 2310 527 526 561 35 TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 526 NA -35 phiX1 + 2310 540 539 574 35 GCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 538 NA -35 phiX1 + 2310 614 613 648 35 TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 613 NA -35 phiX1 + 2310 646 645 680 35 TTCATCCCGTCAACATTCAAACGGCCTGTCTCATC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCATCCCGTCAACATTCAAACGGCCTGTCTCATC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 645 NA -35 phiX1 + 2310 680 679 714 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 678 NA -35 phiX1 + 2310 680 679 714 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 678 NA -35 phiX1 + 2310 789 788 823 35 GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 788 NA -35 phiX1 + 2310 798 797 832 35 GACGCAGAAGAAAACGTGCGTCAAAAATTACGTGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GACGCAGAAGAAAACGTGCGTCAAAAATTACGTGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 796 NA -35 phiX2 + 3076 11 10 45 35 TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 10 NA -35 phiX2 + 3076 1124 1123 1158 35 GCAAAATGAGACTCAAAAAGAGATTGCTGGCATTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCAAAATGAGACTCAAAAAGAGATTGCTGGCATTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1122 NA -35 phiX2 + 3076 1498 1497 1532 35 GTGTGGTTGATATTTTTCATGGTATTGATAAAGCT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTGTGGTTGATATTTTTCATGGTATTGATAAAGCT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1497 NA -35 phiX2 + 3076 1507 1506 1541 35 ATATTTTTCATGGTATTGATAAAGCTGTTGCCGAT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 ATATTTTTCATGGTATTGATAAAGCTGTTGCCGAT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1506 NA -35 phiX2 + 3076 1524 1523 1558 35 GATAAAGCTGTTGCCGATACTTGGAACAATTTCTG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GATAAAGCTGTTGCCGATACTTGGAACAATTTCTG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1523 NA -35 phiX2 + 3076 1542 1541 1576 35 ACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1540 NA -35 phiX2 + 3076 157 156 191 35 CCAGCTTCTTCGGCACCTGTTTTACAGACACCTAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCAGCTTCTTCGGCACCTGTTTTACAGACACCTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 155 NA -35 phiX2 + 3076 1778 1777 1812 35 TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1777 NA -35 phiX2 + 3076 1888 1887 1922 35 ATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGT HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1886 NA -35 phiX2 + 3076 1925 1924 1959 35 TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1924 NA -35 phiX2 + 3076 1985 1984 2019 35 CCCCCAACTTGATATTAATAACACTATAGACCACC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCCCCAACTTGATATTAATAACACTATAGACCACC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1983 NA -35 phiX2 + 3076 1997 1996 2031 35 TATTAATAACACTATAGACCACCGCCCCGAAGGGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TATTAATAACACTATAGACCACCGCCCCGAAGGGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1996 NA -35 phiX2 + 3076 2222 2221 2256 35 GATGAATGCAATGCGACAGGCTCATGCTGATGGTT HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GATGAATGCAATGCGACAGGCTCATGCTGATGGTT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2220 NA -35 phiX2 + 3076 2277 2276 2311 35 CTCACGTTGGCTGACGACCGATTAGAGGCGTTTTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CTCACGTTGGCTGACGACCGATTAGAGGCGTTTTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2275 NA -35 phiX2 + 3076 2309 2308 2343 35 TTATGATAATCCCAATGCTTTGCGTGACTATTTTC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTATGATAATCCCAATGCTTTGCGTGACTATTTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2308 NA -35 phiX2 + 3076 2348 2347 2382 35 TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2347 NA -35 phiX2 + 3076 2349 2348 2383 35 ATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2347 NA -35 phiX2 + 3076 2357 2356 2391 35 TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2356 NA -35 phiX2 + 3076 2578 2577 2612 35 ACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2576 NA -35 phiX2 + 3076 2601 2600 2635 35 TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2600 NA -35 phiX2 + 3076 2816 2815 2850 35 CAGAATCAGAATGAGCCGCAACTTCGGGATGAAAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CAGAATCAGAATGAGCCGCAACTTCGGGATGAAAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2814 NA -35 phiX2 + 3076 2858 2857 2892 35 AATGACAAATCTGTCCACGGAGTGCTTAATCCAAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 AATGACAAATCTGTCCACGGAGTGCTTAATCCAAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2856 NA -35 phiX2 + 3076 2881 2880 2915 35 GCTTAATCCAACTTACCAAGCTGGGTTACGACGCG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GCTTAATCCAACTTACCAAGCTGGGTTACGACGCG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2880 NA -35 phiX2 + 3076 393 392 427 35 CCCGACTGCCTATGATGTTTATCCTTTGAATGGTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCCGACTGCCTATGATGTTTATCCTTTGAATGGTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 391 NA -35 phiX2 + 3076 420 419 454 35 GAATGGTCGCCATGATGGTGGTTATTATACCGTCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GAATGGTCGCCATGATGGTGGTTATTATACCGTCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 419 NA -35 phiX2 + 3076 433 432 467 35 GATGGTGGTTATTATACCGTCAAGGACTGTGTGAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GATGGTGGTTATTATACCGTCAAGGACTGTGTGAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 431 NA -35 phiX2 + 3076 440 439 474 35 GTTATTATACCGTCAAGGACTGTGTGACTATTGAC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTTATTATACCGTCAAGGACTGTGTGACTATTGAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 439 NA -35 phiX2 + 3076 453 452 487 35 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 451 NA -35 phiX2 + 3076 502 501 536 35 GTTTATGTTGGTTTCATGGTTTGGTCTAACTTTAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GTTTATGTTGGTTTCATGGTTTGGTCTAACTTTAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 500 NA -35 phiX2 + 3076 533 532 567 35 TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 532 NA -35 phiX2 + 3076 547 546 581 35 TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 546 NA -35 phiX2 + 3076 597 596 631 35 CCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 595 NA -35 phiX2 + 3076 654 653 688 35 GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 653 NA -35 phiX2 + 3076 735 734 769 35 GCTACCGATAACAATACTGTAGGCATGGGTGATGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCTACCGATAACAATACTGTAGGCATGGGTGATGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 733 NA -35 phiX2 + 3076 756 755 790 35 GGCATGGGTGATGCTGGTATTAAATCTGCCATTCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GGCATGGGTGATGCTGGTATTAAATCTGCCATTCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 755 NA -35 phiX2 + 3076 816 815 850 35 GAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 814 NA -36 phiX1 + 2310 1002 1001 1037 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1001 NA -36 phiX1 + 2310 1002 1001 1037 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1001 NA -36 phiX1 + 2310 1012 1011 1047 36 TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1011 NA -36 phiX1 + 2310 1014 1013 1049 36 AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1013 NA -36 phiX1 + 2310 1082 1081 1117 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1081 NA -36 phiX1 + 2310 1082 1081 1117 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1081 NA -36 phiX1 + 2310 1082 1081 1117 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1081 NA -36 phiX1 + 2310 1091 1090 1126 36 ATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1090 NA -36 phiX1 + 2310 1115 1114 1150 36 GGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1114 NA -36 phiX1 + 2310 1116 1115 1151 36 GCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1115 NA -36 phiX1 + 2310 1144 1143 1179 36 CGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1143 NA -36 phiX1 + 2310 1155 1154 1190 36 TTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1154 NA -36 phiX1 + 2310 1193 1192 1228 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1192 NA -36 phiX1 + 2310 1193 1192 1228 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1192 NA -36 phiX1 + 2310 1207 1206 1242 36 TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1206 NA -36 phiX1 + 2310 1214 1213 1249 36 GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1213 NA -36 phiX1 + 2310 1224 1223 1259 36 GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1223 NA -36 phiX1 + 2310 1229 1228 1264 36 GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1228 NA -36 phiX1 + 2310 1242 1241 1277 36 AGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1241 NA -36 phiX1 + 2310 1249 1248 1284 36 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1248 NA -36 phiX1 + 2310 1252 1251 1287 36 GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1251 NA -36 phiX1 + 2310 1265 1264 1300 36 GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1264 NA -36 phiX1 + 2310 1332 1331 1367 36 GCACGATTAACCCTGATACCAATAAAATCCCTAAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCACGATTAACCCTGATACCAATAAAATCCCTAAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1331 NA -36 phiX1 + 2310 1364 1363 1399 36 AAGCATTTGTTTCAGGGTTATTTGAATATCTATAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGCATTTGTTTCAGGGTTATTTGAATATCTATAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1363 NA -36 phiX1 + 2310 1461 1460 1496 36 AAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1460 NA -36 phiX1 + 2310 1514 1513 1549 36 GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1513 NA -36 phiX1 + 2310 1522 1521 1557 36 TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1521 NA -36 phiX1 + 2310 1529 1528 1564 36 GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1528 NA -36 phiX1 + 2310 1536 1535 1571 36 AGCTTTCTCGCCAAATGACGACTTCTACCACATCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGCTTTCTCGCCAAATGACGACTTCTACCACATCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1535 NA -36 phiX1 + 2310 154 153 189 36 TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 153 NA -36 phiX1 + 2310 1545 1544 1580 36 GCCAAATGACGACTTCTACCACATCTATTGACATTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCCAAATGACGACTTCTACCACATCTATTGACATTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1544 NA -36 phiX1 + 2310 1554 1553 1589 36 CGACTTCTACCACATCTATTGACATTATGGGTCTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGACTTCTACCACATCTATTGACATTATGGGTCTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1553 NA -36 phiX1 + 2310 156 155 191 36 GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 155 NA -36 phiX1 + 2310 157 156 192 36 CGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 156 NA -36 phiX1 + 2310 1575 1574 1610 36 ACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1574 NA -36 phiX1 + 2310 1579 1578 1614 36 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1578 NA -36 phiX1 + 2310 1580 1579 1615 36 ATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1579 NA -36 phiX1 + 2310 1582 1581 1617 36 GGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1581 NA -36 phiX1 + 2310 1587 1586 1622 36 TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1586 NA -36 phiX1 + 2310 1588 1587 1623 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1587 NA -36 phiX1 + 2310 1588 1587 1623 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1587 NA -36 phiX1 + 2310 1590 1589 1625 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1589 NA -36 phiX1 + 2310 1590 1589 1625 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1589 NA -36 phiX1 + 2310 161 160 196 36 GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 160 NA -36 phiX1 + 2310 1655 1654 1690 36 GTTATTTCTTCATTTGGAGGTAAAACCTCTTATGAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTTATTTCTTCATTTGGAGGTAAAACCTCTTATGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1654 NA -36 phiX1 + 2310 1657 1656 1692 36 TATTTCTTCATTTGGAGGTAAAACCTCTTATGACGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATTTCTTCATTTGGAGGTAAAACCTCTTATGACGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1656 NA -36 phiX1 + 2310 1662 1661 1697 36 CTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1661 NA -36 phiX1 + 2310 1665 1664 1700 36 CATTTGGAGGTAAAACCTCTTATGACGCTGACAACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATTTGGAGGTAAAACCTCTTATGACGCTGACAACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1664 NA -36 phiX1 + 2310 1670 1669 1705 36 GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1669 NA -36 phiX1 + 2310 1674 1673 1709 36 GTAAAACCTCTTATGACGCTGACAACCGTCCTTTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTAAAACCTCTTATGACGCTGACAACCGTCCTTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1673 NA -36 phiX1 + 2310 1683 1682 1718 36 CTTATGACGCTGACAACCGTCCTTTACTTGTCATGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATGACGCTGACAACCGTCCTTTACTTGTCATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1682 NA -36 phiX1 + 2310 1690 1689 1725 36 CGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1689 NA -36 phiX1 + 2310 1701 1700 1736 36 GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1700 NA -36 phiX1 + 2310 1707 1706 1742 36 TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1706 NA -36 phiX1 + 2310 1719 1718 1754 36 GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1718 NA -36 phiX1 + 2310 1721 1720 1756 36 TCTAATCTCTGGGCATCTGGCTATGATGTTGATGGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCTAATCTCTGGGCATCTGGCTATGATGTTGATGGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1720 NA -36 phiX1 + 2310 1728 1727 1763 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1727 NA -36 phiX1 + 2310 1728 1727 1763 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1727 NA -36 phiX1 + 2310 1734 1733 1769 36 CATCTGGCTATGATGTTGATGGAACTGACCAAACGT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATCTGGCTATGATGTTGATGGAACTGACCAAACGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1733 NA -36 phiX1 + 2310 1764 1763 1799 36 AAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1763 NA -36 phiX1 + 2310 177 176 212 36 CTTTGCGACCTTTCGCCATCAACTAACGATTCTGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTTGCGACCTTTCGCCATCAACTAACGATTCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 176 NA -36 phiX1 + 2310 1775 1774 1810 36 GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1774 NA -36 phiX1 + 2310 1788 1787 1823 36 GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1787 NA -36 phiX1 + 2310 1800 1799 1835 36 AGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1799 NA -36 phiX1 + 2310 1828 1827 1863 36 CTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1827 NA -36 phiX1 + 2310 1843 1842 1878 36 TGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1842 NA -36 phiX1 + 2310 1852 1851 1887 36 GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1851 NA -36 phiX1 + 2310 1867 1866 1902 36 TGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1866 NA -36 phiX1 + 2310 1888 1887 1923 36 GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1887 NA -36 phiX1 + 2310 1946 1945 1981 36 GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1945 NA -36 phiX1 + 2310 1948 1947 1983 36 CGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1947 NA -36 phiX1 + 2310 1954 1953 1989 36 TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1953 NA -36 phiX1 + 2310 1957 1956 1992 36 TTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1956 NA -36 phiX1 + 2310 1974 1973 2009 36 CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1973 NA -36 phiX1 + 2310 2004 2003 2039 36 TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2003 NA -36 phiX1 + 2310 2059 2058 2094 36 TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2058 NA -36 phiX1 + 2310 206 205 241 36 TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 205 NA -36 phiX1 + 2310 2071 2070 2106 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2070 NA -36 phiX1 + 2310 2071 2070 2106 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2070 NA -36 phiX1 + 2310 2099 2098 2134 36 GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2098 NA -36 phiX1 + 2310 2100 2099 2135 36 AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2099 NA -36 phiX1 + 2310 2116 2115 2151 36 TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2115 NA -36 phiX1 + 2310 2128 2127 2163 36 TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2127 NA -36 phiX1 + 2310 2154 2153 2189 36 TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2153 NA -36 phiX1 + 2310 2160 2159 2195 36 GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2159 NA -36 phiX1 + 2310 2175 2174 2210 36 ACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2174 NA -36 phiX1 + 2310 22 21 57 36 GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 21 NA -36 phiX1 + 2310 220 219 255 36 GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 219 NA -36 phiX1 + 2310 2217 2216 2252 36 AGGTTAAATTTAATGTGACCGTTTATCGCAATCTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGTTAAATTTAATGTGACCGTTTATCGCAATCTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2216 NA -36 phiX1 + 2310 2225 2224 2260 36 TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2224 NA -36 phiX1 + 2310 2239 2238 2274 36 TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2238 NA -36 phiX1 + 2310 227 226 262 36 TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 226 NA -36 phiX1 + 2310 229 228 264 36 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 228 NA -36 phiX1 + 2310 231 230 266 36 TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 230 NA -36 phiX1 + 2310 242 241 277 36 GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 241 NA -36 phiX1 + 2310 257 256 292 36 GCACGTTCGTCAAGGACTGGTTTAGATATGAGTCAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCACGTTCGTCAAGGACTGGTTTAGATATGAGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 256 NA -36 phiX1 + 2310 286 285 321 36 GAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 285 NA -36 phiX1 + 2310 289 288 324 36 TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 288 NA -36 phiX1 + 2310 305 304 340 36 GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 304 NA -36 phiX1 + 2310 318 317 353 36 TGTTGACATTTTAAAAGAGCGTGGATTACTATCTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTTGACATTTTAAAAGAGCGTGGATTACTATCTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 317 NA -36 phiX1 + 2310 332 331 367 36 AAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 331 NA -36 phiX1 + 2310 333 332 368 36 AGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 332 NA -36 phiX1 + 2310 338 337 373 36 GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 337 NA -36 phiX1 + 2310 339 338 374 36 TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 338 NA -36 phiX1 + 2310 348 347 383 36 ATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 347 NA -36 phiX1 + 2310 349 348 384 36 TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 348 NA -36 phiX1 + 2310 379 378 414 36 GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 378 NA -36 phiX1 + 2310 388 387 423 36 TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 387 NA -36 phiX1 + 2310 409 408 444 36 AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 408 NA -36 phiX1 + 2310 441 440 476 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 440 NA -36 phiX1 + 2310 441 440 476 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 440 NA -36 phiX1 + 2310 485 484 520 36 TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 484 NA -36 phiX1 + 2310 496 495 531 36 TTCTGACGAGTAACAAAGTTTGGATTGCTACTGACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCTGACGAGTAACAAAGTTTGGATTGCTACTGACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 495 NA -36 phiX1 + 2310 513 512 548 36 GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 512 NA -36 phiX1 + 2310 521 520 556 36 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 520 NA -36 phiX1 + 2310 524 523 559 36 TACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 523 NA -36 phiX1 + 2310 533 532 568 36 CTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 532 NA -36 phiX1 + 2310 54 53 89 36 AGTCGAAAAATTATCTTGATAAAGCAGGAATTACTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTCGAAAAATTATCTTGATAAAGCAGGAATTACTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 53 NA -36 phiX1 + 2310 555 554 590 36 GAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 554 NA -36 phiX1 + 2310 557 556 592 36 GGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 556 NA -36 phiX1 + 2310 574 573 609 36 CGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 573 NA -36 phiX1 + 2310 584 583 619 36 TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 583 NA -36 phiX1 + 2310 611 610 646 36 TGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 610 NA -36 phiX1 + 2310 613 612 648 36 TTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 612 NA -36 phiX1 + 2310 615 614 650 36 GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 614 NA -36 phiX1 + 2310 632 631 667 36 CATTGCTTATTATGTTCATCCCGTCAACATTCAAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATTGCTTATTATGTTCATCCCGTCAACATTCAAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 631 NA -36 phiX1 + 2310 635 634 670 36 TGCTTATTATGTTCATCCCGTCAACATTCAAACGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGCTTATTATGTTCATCCCGTCAACATTCAAACGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 634 NA -36 phiX1 + 2310 636 635 671 36 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 635 NA -36 phiX1 + 2310 640 639 675 36 ATTATGTTCATCCCGTCAACATTCAAACGGCCTGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTATGTTCATCCCGTCAACATTCAAACGGCCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 639 NA -36 phiX1 + 2310 660 659 695 36 ATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 659 NA -36 phiX1 + 2310 72 71 107 36 ATAAAGCAGGAATTACTACTGCTTGTTTACGAATTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATAAAGCAGGAATTACTACTGCTTGTTTACGAATTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 71 NA -36 phiX1 + 2310 727 726 762 36 GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 726 NA -36 phiX1 + 2310 772 771 807 36 GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 771 NA -36 phiX1 + 2310 852 851 887 36 CTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 851 NA -36 phiX1 + 2310 900 899 935 36 TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 899 NA -36 phiX1 + 2310 910 909 945 36 TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 909 NA -36 phiX1 + 2310 945 944 980 36 TAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 944 NA -36 phiX1 + 2310 962 961 997 36 TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 961 NA -36 phiX1 + 2310 989 988 1024 36 TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 988 NA -36 phiX2 + 3076 1001 1000 1036 36 GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1000 NA -36 phiX2 + 3076 1003 1002 1038 36 TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1002 NA -36 phiX2 + 3076 1016 1015 1051 36 GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1015 NA -36 phiX2 + 3076 1034 1033 1069 36 TTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1033 NA -36 phiX2 + 3076 1058 1057 1093 36 CGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1057 NA -36 phiX2 + 3076 1085 1084 1120 36 TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1084 NA -36 phiX2 + 3076 1098 1097 1133 36 GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1097 NA -36 phiX2 + 3076 1110 1109 1145 36 GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1109 NA -36 phiX2 + 3076 1149 1148 1184 36 GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1148 NA -36 phiX2 + 3076 1154 1153 1189 36 CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1153 NA -36 phiX2 + 3076 1162 1161 1197 36 CGGCGACTTCACGCCAGAATACGAAAGACCAGGTAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGGCGACTTCACGCCAGAATACGAAAGACCAGGTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1161 NA -36 phiX2 + 3076 1194 1193 1229 36 GTATATGCACAAAATGAGATGCTTGCTTATCAACAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATATGCACAAAATGAGATGCTTGCTTATCAACAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1193 NA -36 phiX2 + 3076 1210 1209 1245 36 AGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1209 NA -36 phiX2 + 3076 1212 1211 1247 36 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1211 NA -36 phiX2 + 3076 1230 1229 1265 36 AAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1229 NA -36 phiX2 + 3076 1234 1233 1269 36 AGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1233 NA -36 phiX2 + 3076 1253 1252 1288 36 GTCTATTATGGAAAACACCAATCTTTCCAAGCAACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTCTATTATGGAAAACACCAATCTTTCCAAGCAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1252 NA -36 phiX2 + 3076 126 125 161 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 125 NA -36 phiX2 + 3076 126 125 161 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 125 NA -36 phiX2 + 3076 1260 1259 1295 36 ATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1259 NA -36 phiX2 + 3076 1262 1261 1297 36 GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1261 NA -36 phiX2 + 3076 1263 1262 1298 36 GAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1262 NA -36 phiX2 + 3076 1277 1276 1312 36 TTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1276 NA -36 phiX2 + 3076 1282 1281 1317 36 AGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1281 NA -36 phiX2 + 3076 1290 1289 1325 36 CAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1289 NA -36 phiX2 + 3076 1307 1306 1342 36 GCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1306 NA -36 phiX2 + 3076 1309 1308 1344 36 GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1308 NA -36 phiX2 + 3076 1316 1315 1351 36 GCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1315 NA -36 phiX2 + 3076 1319 1318 1354 36 TACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1318 NA -36 phiX2 + 3076 132 131 167 36 TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 131 NA -36 phiX2 + 3076 1364 1363 1399 36 CAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1363 NA -36 phiX2 + 3076 1389 1388 1424 36 GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1388 NA -36 phiX2 + 3076 1398 1397 1433 36 GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1397 NA -36 phiX2 + 3076 1402 1401 1437 36 TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1401 NA -36 phiX2 + 3076 1421 1420 1456 36 GAATCAGCGGTATGGCTCTTCTCATATTGGCGCTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAATCAGCGGTATGGCTCTTCTCATATTGGCGCTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1420 NA -36 phiX2 + 3076 143 142 178 36 TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 142 NA -36 phiX2 + 3076 1446 1445 1481 36 ATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1445 NA -36 phiX2 + 3076 1451 1450 1486 36 CGCTACTGCAAAGGATATTTCTAATGTCGTCACTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTACTGCAAAGGATATTTCTAATGTCGTCACTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1450 NA -36 phiX2 + 3076 1477 1476 1512 36 TCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1476 NA -36 phiX2 + 3076 1539 1538 1574 36 GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1538 NA -36 phiX2 + 3076 1548 1547 1583 36 AACAATTTCTGGAAAGACGGTAAAGCTGATGGTATT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AACAATTTCTGGAAAGACGGTAAAGCTGATGGTATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1547 NA -36 phiX2 + 3076 1551 1550 1586 36 AATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1550 NA -36 phiX2 + 3076 1552 1551 1587 36 ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1551 NA -36 phiX2 + 3076 1553 1552 1588 36 TTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1552 NA -36 phiX2 + 3076 1610 1609 1645 36 GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1609 NA -36 phiX2 + 3076 1668 1667 1703 36 TTTATGGTTCGTTCTTATTACCCTTCTGAATGTCAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTATGGTTCGTTCTTATTACCCTTCTGAATGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1667 NA -36 phiX2 + 3076 1669 1668 1704 36 TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1668 NA -36 phiX2 + 3076 1672 1671 1707 36 TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1671 NA -36 phiX2 + 3076 1678 1677 1713 36 GTTCTTATTACCCTTCTGAATGTCACGCTGATTATT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTTCTTATTACCCTTCTGAATGTCACGCTGATTATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1677 NA -36 phiX2 + 3076 169 168 204 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 168 NA -36 phiX2 + 3076 169 168 204 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 168 NA -36 phiX2 + 3076 1690 1689 1725 36 CTTCTGAATGTCACGCTGATTATTTTGACTTTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTCTGAATGTCACGCTGATTATTTTGACTTTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1689 NA -36 phiX2 + 3076 1695 1694 1730 36 GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1694 NA -36 phiX2 + 3076 1716 1715 1751 36 GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1715 NA -36 phiX2 + 3076 1729 1728 1764 36 TCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1728 NA -36 phiX2 + 3076 1733 1732 1768 36 GGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1732 NA -36 phiX2 + 3076 1753 1752 1788 36 AGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1752 NA -36 phiX2 + 3076 1785 1784 1820 36 CCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1784 NA -36 phiX2 + 3076 1788 1787 1823 36 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1787 NA -36 phiX2 + 3076 1789 1788 1824 36 TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1788 NA -36 phiX2 + 3076 1816 1815 1851 36 ACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1815 NA -36 phiX2 + 3076 1820 1819 1855 36 CATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1819 NA -36 phiX2 + 3076 1822 1821 1857 36 TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1821 NA -36 phiX2 + 3076 1860 1859 1895 36 GGCGTTGAGTTCGATAATGGTGATATGTATGTTGAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCGTTGAGTTCGATAATGGTGATATGTATGTTGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1859 NA -36 phiX2 + 3076 1864 1863 1899 36 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1863 NA -36 phiX2 + 3076 1866 1865 1901 36 GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1865 NA -36 phiX2 + 3076 1882 1881 1917 36 ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1881 NA -36 phiX2 + 3076 1886 1885 1921 36 GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1885 NA -36 phiX2 + 3076 1893 1892 1928 36 GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1892 NA -36 phiX2 + 3076 1907 1906 1942 36 TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1906 NA -36 phiX2 + 3076 1938 1937 1973 36 GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1937 NA -36 phiX2 + 3076 1939 1938 1974 36 TTACTGAGAAGTTAATGGATGAATTGGCACAATGCT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTACTGAGAAGTTAATGGATGAATTGGCACAATGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1938 NA -36 phiX2 + 3076 1944 1943 1979 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1943 NA -36 phiX2 + 3076 1944 1943 1979 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1943 NA -36 phiX2 + 3076 1946 1945 1981 36 GAAGTTAATGGATGAATTGGCACAATGCTACAATGT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAAGTTAATGGATGAATTGGCACAATGCTACAATGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1945 NA -36 phiX2 + 3076 1950 1949 1985 36 TTAATGGATGAATTGGCACAATGCTACAATGTGCTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAATGGATGAATTGGCACAATGCTACAATGTGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1949 NA -36 phiX2 + 3076 1972 1971 2007 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1971 NA -36 phiX2 + 3076 1972 1971 2007 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1971 NA -36 phiX2 + 3076 2071 2070 2106 36 AGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2070 NA -36 phiX2 + 3076 2094 2093 2129 36 GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2093 NA -36 phiX2 + 3076 2104 2103 2139 36 TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2103 NA -36 phiX2 + 3076 2118 2117 2153 36 GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2117 NA -36 phiX2 + 3076 2124 2123 2159 36 TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2123 NA -36 phiX2 + 3076 2130 2129 2165 36 TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2129 NA -36 phiX2 + 3076 2161 2160 2196 36 GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2160 NA -36 phiX2 + 3076 2229 2228 2264 36 GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2228 NA -36 phiX2 + 3076 2241 2240 2276 36 GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2240 NA -36 phiX2 + 3076 2250 2249 2285 36 GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2249 NA -36 phiX2 + 3076 2268 2267 2303 36 TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2267 NA -36 phiX2 + 3076 2286 2285 2321 36 GCTGACGACCGATTAGAGGCGTTTTATGATAATCCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTGACGACCGATTAGAGGCGTTTTATGATAATCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2285 NA -36 phiX2 + 3076 2296 2295 2331 36 GATTAGAGGCGTTTTATGATAATCCCAATGCTTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GATTAGAGGCGTTTTATGATAATCCCAATGCTTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2295 NA -36 phiX2 + 3076 2298 2297 2333 36 TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2297 NA -36 phiX2 + 3076 2374 2373 2409 36 AGGGTCGCAAGGCTAATGATTCACACGCCGACTGCT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGGTCGCAAGGCTAATGATTCACACGCCGACTGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2373 NA -36 phiX2 + 3076 2380 2379 2415 36 GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2379 NA -36 phiX2 + 3076 2430 2429 2465 36 GAGTATGGTACAGCTAATGGCCGTCTTCATTTCCAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAGTATGGTACAGCTAATGGCCGTCTTCATTTCCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2429 NA -36 phiX2 + 3076 247 246 282 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 246 NA -36 phiX2 + 3076 247 246 282 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 246 NA -36 phiX2 + 3076 2478 2477 2513 36 ATGCGGACACTTCCTACAGGTAGCGTTGACCCTAAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCGGACACTTCCTACAGGTAGCGTTGACCCTAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2477 NA -36 phiX2 + 3076 2482 2481 2517 36 GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2481 NA -36 phiX2 + 3076 2530 2529 2565 36 GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2529 NA -36 phiX2 + 3076 2555 2554 2590 36 GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2554 NA -36 phiX2 + 3076 2556 2555 2591 36 CAAAATACGTGGCCTTATGGTTACAGTATGCCCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAAATACGTGGCCTTATGGTTACAGTATGCCCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2555 NA -36 phiX2 + 3076 2574 2573 2609 36 GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2573 NA -36 phiX2 + 3076 2581 2580 2616 36 GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2580 NA -36 phiX2 + 3076 2599 2598 2634 36 GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2598 NA -36 phiX2 + 3076 2626 2625 2661 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2625 NA -36 phiX2 + 3076 2626 2625 2661 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2625 NA -36 phiX2 + 3076 2627 2626 2662 36 TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2626 NA -36 phiX2 + 3076 2635 2634 2670 36 TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2634 NA -36 phiX2 + 3076 264 263 299 36 TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 263 NA -36 phiX2 + 3076 2640 2639 2675 36 CCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2639 NA -36 phiX2 + 3076 2645 2644 2680 36 TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2644 NA -36 phiX2 + 3076 2647 2646 2682 36 ATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2646 NA -36 phiX2 + 3076 267 266 302 36 GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 266 NA -36 phiX2 + 3076 2671 2670 2706 36 CTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2670 NA -36 phiX2 + 3076 2695 2694 2730 36 TCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2694 NA -36 phiX2 + 3076 2723 2722 2758 36 GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2722 NA -36 phiX2 + 3076 2729 2728 2764 36 TATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2728 NA -36 phiX2 + 3076 2742 2741 2777 36 GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2741 NA -36 phiX2 + 3076 2763 2762 2798 36 GAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2762 NA -36 phiX2 + 3076 2767 2766 2802 36 GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2766 NA -36 phiX2 + 3076 2789 2788 2824 36 GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2788 NA -36 phiX2 + 3076 2795 2794 2830 36 GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2794 NA -36 phiX2 + 3076 2797 2796 2832 36 TACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2796 NA -36 phiX2 + 3076 282 281 317 36 CAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 281 NA -36 phiX2 + 3076 2841 2840 2876 36 GGGATGAAAATGCTCACAATGACAAATCTGTCCACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGGATGAAAATGCTCACAATGACAAATCTGTCCACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2840 NA -36 phiX2 + 3076 2842 2841 2877 36 GGATGAAAATGCTCACAATGACAAATCTGTCCACGG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGATGAAAATGCTCACAATGACAAATCTGTCCACGG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2841 NA -36 phiX2 + 3076 2847 2846 2882 36 AAAATGCTCACAATGACAAATCTGTCCACGGAGTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAAATGCTCACAATGACAAATCTGTCCACGGAGTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2846 NA -36 phiX2 + 3076 2852 2851 2887 36 GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2851 NA -36 phiX2 + 3076 2866 2865 2901 36 ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2865 NA -36 phiX2 + 3076 2869 2868 2904 36 TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2868 NA -36 phiX2 + 3076 2877 2876 2912 36 GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2876 NA -36 phiX2 + 3076 2896 2895 2931 36 CCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2895 NA -36 phiX2 + 3076 2962 2961 2997 36 GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2961 NA -36 phiX2 + 3076 311 310 346 36 GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 310 NA -36 phiX2 + 3076 358 357 393 36 GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 357 NA -36 phiX2 + 3076 379 378 414 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 378 NA -36 phiX2 + 3076 379 378 414 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 378 NA -36 phiX2 + 3076 388 387 423 36 ACCCTCCCGACTGCCTATGATGTTTATCCTTTGAAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCCTCCCGACTGCCTATGATGTTTATCCTTTGAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 387 NA -36 phiX2 + 3076 400 399 435 36 GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 399 NA -36 phiX2 + 3076 418 417 453 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 417 NA -36 phiX2 + 3076 418 417 453 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 417 NA -36 phiX2 + 3076 428 427 463 36 GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 427 NA -36 phiX2 + 3076 439 438 474 36 GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 438 NA -36 phiX2 + 3076 444 443 479 36 TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 443 NA -36 phiX2 + 3076 446 445 481 36 ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 445 NA -36 phiX2 + 3076 452 451 487 36 TCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 451 NA -36 phiX2 + 3076 453 452 488 36 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 452 NA -36 phiX2 + 3076 456 455 491 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 455 NA -36 phiX2 + 3076 456 455 491 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 455 NA -36 phiX2 + 3076 465 464 500 36 GACTATTGACGTCCTTCCCCGTACGCCGGGCAATAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GACTATTGACGTCCTTCCCCGTACGCCGGGCAATAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 464 NA -36 phiX2 + 3076 505 504 540 36 TATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 504 NA -36 phiX2 + 3076 506 505 541 36 ATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 505 NA -36 phiX2 + 3076 540 539 575 36 TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 539 NA -36 phiX2 + 3076 553 552 588 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 552 NA -36 phiX2 + 3076 553 552 588 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 552 NA -36 phiX2 + 3076 563 562 598 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 562 NA -36 phiX2 + 3076 563 562 598 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 562 NA -36 phiX2 + 3076 569 568 604 36 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 568 NA -36 phiX2 + 3076 624 623 659 36 TTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 623 NA -36 phiX2 + 3076 633 632 668 36 ATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 632 NA -36 phiX2 + 3076 691 690 726 36 GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 690 NA -36 phiX2 + 3076 705 704 740 36 GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 704 NA -36 phiX2 + 3076 71 70 106 36 TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 70 NA -36 phiX2 + 3076 711 710 746 36 GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 710 NA -36 phiX2 + 3076 772 771 807 36 GTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 771 NA -36 phiX2 + 3076 783 782 818 36 GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 782 NA -36 phiX2 + 3076 786 785 821 36 ATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 785 NA -36 phiX2 + 3076 790 789 825 36 AAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 789 NA -36 phiX2 + 3076 809 808 844 36 CCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 808 NA -36 phiX2 + 3076 817 816 852 36 AGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 816 NA -36 phiX2 + 3076 841 840 876 36 GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 840 NA -36 phiX2 + 3076 857 856 892 36 TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 856 NA -36 phiX2 + 3076 903 902 938 36 GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 902 NA -36 phiX2 + 3076 940 939 975 36 GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 939 NA -36 phiX2 + 3076 951 950 986 36 GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 950 NA -36 phiX2 + 3076 961 960 996 36 GAAAGGATACTCGTGATTATCTTGCTGCTGCATTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAAAGGATACTCGTGATTATCTTGCTGCTGCATTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 960 NA -36 phiX2 + 3076 967 966 1002 36 ATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 966 NA -36 phiX2 + 3076 968 967 1003 36 TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 967 NA -36 phiX2 + 3076 974 973 1009 36 TGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 973 NA
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/score_file.txt Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,12 @@ +# This matches the default scoring set for BLASTZ + +bad_score = X:-1000 # used for sub['X'][*] and sub[*]['X'] +fill_score = -100 # used when sub[*][*] is not defined +gap_open_penalty = 400 +gap_extend_penalty = 30 + + A C G T +A 91 -114 -31 -123 +C -114 100 -125 -31 +G -31 -125 100 -114 +T -123 -31 -114 91
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test1.out Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,2 @@ +219263 phiX174 + 5386 0 2310 phiX1 + 2310 0 2310 2308/2310 99.9% 2310/2310 100.0% +291916 phiX174 + 5386 2310 5386 phiX2 + 3076 0 3076 3073/3076 99.9% 3076/3076 100.0%
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test2.out Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,2 @@ +219263 phiX1 + 2310 0 2310 phiX174 + 5386 0 2310 2308/2310 99.9% 2310/2310 100.0% +291916 phiX2 + 3076 0 3076 phiX174 + 5386 2310 5386 3073/3076 99.9% 3076/3076 100.0%
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test3.out Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,2 @@ +219263 phiX1 + 2310 0 2310 phiX174 + 5386 0 2310 2308/2310 99.9% 2310/2310 100.0% +291916 phiX2 + 3076 0 3076 phiX174 + 5386 2310 5386 3073/3076 99.9% 3076/3076 100.0%
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_data_table_conf.xml.test Fri Mar 02 12:06:35 2018 -0500 @@ -0,0 +1,8 @@ +<!-- Use the file tool_data_table_conf.xml.oldlocstyle if you don't want to update your loc files as changed in revision 4550:535d276c92bc--> +<tables> + <!-- Locations of 2bit sequence files for use in Lastz --> + <table name="lastz_seqs" comment_char="#"> + <columns>value, name, path</columns> + <file path="${__HERE__}/test-data/lastz_seqs.loc" /> + </table> +</tables>
--- a/tool_dependencies.xml Tue Oct 13 12:23:37 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,6 +0,0 @@ -<?xml version="1.0"?> -<tool_dependency> - <package name="lastz" version="1.02.00"> - <repository changeset_revision="28f0a3faa641" name="package_lastz_1_02_00" owner="devteam" toolshed="https://testtoolshed.g2.bx.psu.edu" /> - </package> -</tool_dependency>