annotate lastz.xml @ 2:8e9252994649 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
author iuc
date Fri, 02 Mar 2018 12:06:35 -0500
parents
children c3767eaae954
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
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8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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1 <tool id="lastz_wrapper_2" name="LASTZ" version="1.3">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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2 <description>: align long sequences</description>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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3 <macros>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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4 <import>lastz_macros.xml</import>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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5 </macros>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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6 <requirements>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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7 <requirement type="package" version="@LASTZ_CONDA_VERSION@">lastz</requirement>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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8 <requirement type="package" version="1.7">samtools</requirement>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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9 <requirement type="package" version="3.4.2">r-base</requirement>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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10 </requirements>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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11 <command detect_errors="exit_code"><![CDATA[
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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12 lastz
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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13 @TARGET_INPUT_COMMAND_LINE@
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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14 ## If --self is set: perform self alignment and ignore the query
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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15 #if $where_to_look.self:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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16 '${where_to_look.self}'
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17 #if $where_to_look.nomirror
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18 '${where_to_look.nomirror}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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19 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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20 #else:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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21 '${query}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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22 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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23
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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24 ## WHERE TO LOOK --------------------------------
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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25
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26 '${where_to_look.strand}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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27 #if str( $where_to_look.qhsplimit.qhsplimit_selector ) == "yes":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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28 #if $where_to_look.qhsplimit.qhsplimit_n:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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29 #if $where_to_look.qhsplimit.qhsplimit_nowarn:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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30 #if $where_to_look.qhsplimit.qhsplimit_keep:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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31 '--queryhsplimit=${where_to_look.qhsplimit.qhsplimit_keep},${where_to_look.qhsplimit.qhsplimit_nowarn}:${where_to_look.qhsplimit.qhsplimit_n}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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32 #else:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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33 '--queryhsplimit=${where_to_look.qhsplimit.qhsplimit_nowarn}:${where_to_look.qhsplimit.qhsplimit_n}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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34 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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35 #else:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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36 '--queryhsplimit=${where_to_look.qhsplimit.qhsplimit_n}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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37 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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38 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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39 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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40
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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41 #if $where_to_look.qhspbest:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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42 '--queryhspbest=${where_to_look.qhspbest}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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43 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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44
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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45 #if str( $where_to_look.qdepth.qdepth_selector ) == "yes":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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46 #if $where_to_look.qdepth.qdepth_n:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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47 #if $where_to_look.qdepth.qdepth_nowarn and not $where_to_look.qdepth.qdepth_keep:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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48 '--querydepth=${where_to_look.qdepth.qdepth_nowarn}:${where_to_look.qdepth.qdepth_n}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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49 #elif not $where_to_look.qdepth.qdepth_nowarn and $where_to_look.qdepth.qdepth_keep:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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50 '--querydepth=${where_to_look.qdepth.qdepth_keep}:${where_to_look.qdepth.qdepth_n}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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51 #elif $where_to_look.qdepth.qdepth_nowarn and $where_to_look.qdepth.qdepth_keep:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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52 '--querydepth=${where_to_look.qdepth.qdepth_keep},${where_to_look.qdepth.qdepth_nowarn}:${where_to_look.qdepth.qdepth_n}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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53 #else:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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54 '--querydepth=${where_to_look.qdepth.qdepth_n}'
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55 #end if
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56 #end if
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57 #end if
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58
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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59 ## SCORING --------------------------------
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60
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61 #if $scoring.score_file:
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62 '--scores=${scoring.score_file}'
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63 #end if
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64
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65 #if str( $scoring.match.match_selector ) == "yes":
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66 #if $scoring.match.match_reward:
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67 #if $scoring.match.match_penalty:
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68 '--match=${scoring.match.match_reward},${scoring.match.match_penalty}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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69 #else:
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70 '--match=${scoring.match.match_reward}'
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71 #end if
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72 #end if
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73 #end if
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74 #if str( $scoring.gap.gap_selector ) == "yes":
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75 #if $scoring.gap.gap_extend:
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76 #if $scoring.gap.gap_open:
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77 '--gap=${scoring.gap.gap_open},${scoring.gap.gap_extend}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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78 #else:
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79 '--gap=${scoring.gap.gap_extend}'
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80 #end if
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81 #end if
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82 #end if
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83 #if $scoring.ambigN:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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84 '${scoring.ambigN}'
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85 #end if
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86 #if $scoring.ambigIUPAC:
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87 '${scoring.ambigIUPAC}'
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88 #end if
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89
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90 ## SEEDING --------------------------------
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
91
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
92 #if str( $seeding.seed.seed_selector ) == "pre_set":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
93 '${seeding.seed.pre_set_seeds}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
94 #elif str( $seeding.seed.seed_selector ) == "len":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
95 '--seed=match${seeding.seed.seed_match}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
96 #elif str( $seeding.seed.seed_selector ) == "half_len":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
97 '--seed=half${seeding.seed.seed_half}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
98 #elif str( $seeding.seed.seed_selector ) == "pattern":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
99 '--seed=${seeding.seed.pattern}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
100 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
101 #if str( $seeding.transitions ) != "--transition":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
102 '${seeding.transitions}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
103 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
104 #if str( $seeding.seed_filer.seed_filer_selector ) == "yes":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
105 #if $seeding.seed_filer.filter_match:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
106 #if $seeding.seed_filer.filter_tr:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
107 '--filter=${seeding.seed_filer.filter_tr},${seeding.seed_filer.filter_match}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
108 #else:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
109 '--filter=${seeding.seed_filer.filter_match}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
110 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
111 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
112 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
113
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
114 ## FINDING HSPs --------------------------------
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
115
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iuc
parents:
diff changeset
116 #if $hsp.gfextend:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
117 '${hsp.gfextend}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
118 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
119 #if $hsp.nogfextend
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
120 '${hsp.nogfextend}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
121 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
122 #if str( $hsp.hsp_method.hsp_method_selector ) == "match":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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diff changeset
123
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
124 #if $hsp.hsp_method.exact:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
125 '--exact=${hsp.hsp_method.exact}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
126 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
127 #elif str( $hsp.hsp_method.hsp_method_selector ) == "mismatch":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
128
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iuc
parents:
diff changeset
129 #if $hsp.hsp_method.mismatch_count and $hsp.hsp_method.mismatch_length:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
130 '--mismatch=${hsp.hsp_method.mismatch_count},${hsp.hsp_method.mismatch_length}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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diff changeset
131 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
132 #elif str( $hsp.hsp_method.hsp_method_selector ) == "x":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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diff changeset
133
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iuc
parents:
diff changeset
134 #if $hsp.hsp_method.xdrop:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
135 '--xdrop=${hsp.hsp_method.xdrop}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
136 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
137
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iuc
parents:
diff changeset
138 #if $hsp.hsp_method.hspthresh:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
139 '--hspthresh=${hsp.hsp_method.hspthresh}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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diff changeset
140 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
141
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iuc
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diff changeset
142 #if $hsp.hsp_method.hspthresh_top:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
143 '--hspthresh=top${hsp.hsp_method.hspthresh_top}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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diff changeset
144 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
145
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iuc
parents:
diff changeset
146 #if $hsp.hsp_method.hspthresh_top_percent:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
147 '--hspthresh=top${hsp.hsp_method.hspthresh_top_percent}%'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
148 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
149 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
150 #if $hsp.entropy:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
151 '${hsp.entropy}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
152 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
153 #if $hsp.entropy_report:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
154 '${hsp.entropy_report}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
155 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
156 #if $hsp.noentropy:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
157 '${hsp.noentropy}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
158 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
159
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
160 ## CHAINING --------------------------------
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
161
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
162 #if $chaining.chain:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
163 '${chaining.chain}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
164 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
165 #if str( $chaining.chaning_penalties.chaning_penalties_selector ) == "yes":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
166 #if $chaining.chaning_penalties.diag and $chaining.chaning_penalties.anti:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
167 '--chain=${chaining.chaning_penalties.diag},${chaining.chaning_penalties.anti}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
168 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
169 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
170
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
171 ## GAPPED EXTENSION --------------------------------
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
172
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
173 #if $gap_ext.gapped:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
174 '${gap_ext.gapped}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
175 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
176 #if $gap_ext.nogapped:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
177 '${gap_ext.nogapped}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
178 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
179 #if $gap_ext.ydrop:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
180 '--ydrop=${gap_ext.ydrop}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
181 #end if
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
182 #if $gap_ext.noytrim:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
183 '${gap_ext.noytrim}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
184 #end if
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185 #if $gap_ext.gappedthresh:
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186 '--gappedthresh=${gap_ext.gappedthresh}'
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187 #end if
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188 #if $gap_ext.allgappedbounds:
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189 '${gap_ext.allgappedbounds}'
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190 #end if
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191
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192 ## FILTERING --------------------------------
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193
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194 #if $filters.identity.id_min:
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195 #if $filters.identity.id_max:
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196 '--filter=identity:${filters.identity.id_min}..${filters.identity.id_max}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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197 #else:
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198 '--filter=identity:${filters.identity.id_min}'
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199 #end if
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200 #end if
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201 #if $filters.continuity.cont_min:
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iuc
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202 #if $filters.continuity.cont_max:
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iuc
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203 '--filter=continuity:${filters.continuity.cont_min}..${filters.continuity.cont_max}'
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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204 #else:
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205 '--filter=continuity:${filters.continuity.cont_min}'
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206 #end if
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207 #end if
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208 #if $filters.coverage.cov_min:
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209 #if $filters.coverage.cov_max:
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210 '--filter=coverage:${filters.coverage.cov_min}..${filters.coverage.cov_max}'
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211 #else:
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212 '--filter=coverage:${filters.coverage.cov_min}'
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213 #end if
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214 #end if
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215 #if $filters.filter_nmatch:
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216 '--filter=nmatch:${filters.filter_nmatch}'
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217 #end if
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218 #if $filters.filter_nmatch_percent:
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219 '--filter=nmatch:${filters.filter_nmatch_percent}%'
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220 #end if
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221 #if $filters.filter_nmismatch:
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222 '--filter=nmismatch:0..${filters.filter_nmismatch}'
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223 #end if
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224 #if $filters.filter_ngap:
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225 '--filter=ngap:0..${filters.filter_ngap}'
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226 #end if
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227 #if $filters.filter_cgap:
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228 '--filter=cgap:0..${filters.filter_cgap}'
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229 #end if
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230 #if $filters.notrivial:
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231 '${filters.notrivial}'
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232 #end if
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233
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234 ## INTERPOLATION --------------------------------
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235
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236 #if $interpolation.inner:
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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237 '--inner=${interpolation.inner}'
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238 #end if
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239
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240 ## OUTPUT FORMATS --------------------------------
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241
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242 #if str( $output_format.out.format ) == "bam":
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243 '--format=${output_format.out.bam_options}'
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244 #elif str( $output_format.out.format ) == "general_def":
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245 --format=general-
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246 #elif str( $output_format.out.format ) == "maf":
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247 '--format=${output_format.out.maf_type}'
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248 #elif str( $output_format.out.format ) == "blastn":
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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249 --format=BLASTN-
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iuc
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250 #elif str( $output_format.out.format ) == "general_full":
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iuc
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diff changeset
251 '--format=general-:${output_format.out.fields}'
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252 #end if
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253 --action:target=multiple
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254 --rdotplot=plot.r
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255 #if str( $output_format.out.format ) == "bam":
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256 | samtools sort -@\${GALAXY_SLOTS:-2} -O bam -o '${output}' &&
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257 #else:
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258 > '${output}' &&
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259 #end if
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260 Rscript $r_plot > /dev/null 2>&1
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261
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262 ]]>
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diff changeset
263 </command>
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parents:
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264 <configfiles>
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265 <configfile name="r_plot">
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266 <![CDATA[
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parents:
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267 #!/usr/bin/env Rscript
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268 ## Setup R error handling to go to stderr
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269 options( show.error.messages=F, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } )
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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270 ## Only produce image when 'plot.r' actually contains data
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271 if(file.info('plot.r')\$size>0){
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272 x <- read.table("plot.r", as.is=TRUE)
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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273 png("${out_plot}", width=640, height=640, res=72)
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274 plot(x, type='l', xlab="target", ylab="query", main="plot")
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275 dev.off()
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276 }
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parents:
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277 ]]>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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278 </configfile>
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279 </configfiles>
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280 <inputs>
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281 <expand macro="target_input"/>
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282 <param name="query" format="fasta,fastq" type="data" label="Select QUERY sequence(s)" help="These are the sequences that you are aligning against TARGET"/>
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283
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284 <section name="where_to_look" expanded="False" title="Where to look">
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285 <param name="strand" type="select" display="radio" label="which strand to search" argument="--strand" help="Search both strands or choose plus or minus">
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286 <option value="--strand=both" selected="True">Both</option>
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287 <option value="--strand=plus">Plus</option>
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288 <option value="--strand=minus">Minus</option>
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289 </param>
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290 <param name="self" type="boolean" display="radio" truevalue="--self" falsevalue="" checked="False" argument="--self" label="Perform a self-alignment: the target sequence is also the query." help="Computation is more efficient than it would be without this option, since only one of each mirror-image pair of alignment blocks is processed (the other, redundant one is skipped during processing, but re-created in the output). Also, the trivial self-alignment block along the main diagonal is omitted from the output. THIS OPTION CANNOT BE USED IF THE TARGET IS COMPRIZED OF MULTIPLE SEQUENCES"/>
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291 <param name="nomirror" type="boolean" display="radio" truevalue="--nomirror" falsevalue="" checked="False" label="Inhibit the re-creation of mirror-image alignments." argument="--nomirror" help="Output consists of only one copy of each meaningful alignment block in a self-alignment. This option is only applicable when the ‑‑self option is used."/>
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292 <conditional name="qhsplimit">
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293 <param name="qhsplimit_selector" type="select" display="radio" label="Set HSP limit" argument="--queryhsplimit">
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294 <option value="yes">Yes</option>
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295 <option value="no" selected="true">No</option>
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296 </param>
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297 <when value="yes">
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298 <param name="qhsplimit_n" optional="true" type="integer" label="Discard queries that have more than N HSPs" help="Any queries that exceed this limit are reported as a warning (to stderr), and no alignments are reported. This is useful for mapping reads to a reference genome, when some reads align to too many places in the reference."/>
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299 <param name="qhsplimit_nowarn" type="boolean" truevalue="nowarn" display="radio" checked="False" label=" Withhold warnings for queries that exceed the limit set above"/>
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300 <param name="qhsplimit_keep" type="boolean" truevalue="keep" display="radio" checked="False" label="Keep queries that exceed the limit and supress warnings" help="For such a query, the first N HSPs found are passed along to downstream processing. Note that the HSPs reported are not the best N HSPs. They are simply the first N found; they very likely have a positional bias."/>
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301 </when>
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302 <when value="no">
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303 <!-- Do nothing -->
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304 </when>
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305 </conditional>
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306 <param name="qhspbest" type="integer" optional="true" label="For queries that have more than N HSPs, discard any HSPs that score below the Nth best." help="This is useful for mapping reads to a reference genome, when some reads align to too many places in the reference."/>
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307 <conditional name="qdepth">
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308 <param name="qdepth_selector" type="select" display="radio" label="Set ratio of aligned bases to query length" argument="--querydepth">
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309 <option value="yes">Yes</option>
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310 <option value="no" selected="true">No</option>
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311 </param>
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312 <when value="yes">
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313 <param name="qdepth_n" optional="true" type="integer" label="Stop processing gapped alignments for a query/strand if its ratio of aligned bases to query length exceeds" help="This is a real number that corresponds to a depth of coverage threshold. For example, a value of 5.0 would cause termination once a query/strand has an average of five alignments for every base in the query. The numerator is the number of matches or substitutions (but not gaps); the denominator is the length of the query sequence. The purpose of this option is one of saving time. It is useful for automatically terminating the processing of queries with high repeat content, for which other methods of dealing with repetitive content fail. Moreover, back-end filtering options are not considered. In other words, matches are counted for any alignment that meets the scoring threshold, regardless of whether that alignment would be reported. The justification is that we are trying to abort the processing of queries that have too many bounding alignments in the DP matrix, and back-end filtering occurs later in the process."/>
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314 <param name="qdepth_keep" type="boolean" truevalue="keep" display="radio" checked="False" label="Warnings for queries that exceed the limit are witheld" help="Note that the alignments reported are not guaranteed to be the highest scoring alignments that would achieve the threshold. They are simply the first alignments found. In other words, the purpose of this option is one of saving time, not one of finding optimal alignments."/>
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315 <param name="qdepth_nowarn" type="boolean" truevalue="nowarn" display="radio" checked="False" label="Same as above but any alignments discovered for this query/strand, before it exceeds the threshold, are reported."/>
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316 </when>
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317 <when value="no">
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318 <!-- Do nothing -->
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319 </when>
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320 </conditional>
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321 </section>
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322 <section name="scoring" expanded="false" title="Scoring">
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323 <param name="score_file" type="data" format="txt" optional="true" argument="--scores" label="Read the substitution scores and gap penalties (and possibly other options) from a scoring file (see help below for file fomat description)." help="This option cannot be used in conjunction with ‑‑match or inference."/> <!--TODO EDIT INFERENCE -->
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324 <conditional name="match">
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325 <param name="match_selector" type="select" display="radio" label="Set the score values for a match and mismatch" argument="--match" help="Note that specifying ‑‑match changes the defaults for some of the other options (e.g. the scoring penalties for gaps, and various extension thresholds), as described in respective sections of LASTZ manual. The regular defaults are chosen for compatibility with BLASTZ, but since BLASTZ doesn't support ‑‑match, LASTZ infers that you are not expecting BLASTZ compatibility for this run, so it is free to use improved defaults. THIS OPTION CANNOT BE USED WITH --scores">
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326 <option value="yes">Yes</option>
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327 <option value="no" selected="true">No</option>
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328 </param>
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329 <when value="yes">
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330 <param name="match_reward" type="integer" optional="true" label="Score value for a match"/>
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331 <param name="match_penalty" type="integer" optional="true" label="Score value for a mismatch"/>
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332 </when>
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333 <when value="no">
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334 <!-- Do nothing -->
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335 </when>
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336 </conditional>
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337 <conditional name="gap">
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338 <param name="gap_selector" type="select" display="radio" label="Set the score penalties for opening and extending a gap" argument="--gap" help="These are specified as positive values; subtraction is implicitly assumed. Note that the first base in a gap incurs the sum of both penalties. This option is only valid if gapped extension is being performed, and cannot be used in conjunction with inference. These values specified on the command line override any corresponding values from a file provided with ‑‑scores.">
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339 <option value="yes">Yes</option>
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340 <option value="no" selected="true">No</option>
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341 </param>
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342 <when value="yes">
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343 <param name="gap_open" type="integer" optional="true" label="Gap opening penalty"/>
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344 <param name="gap_extend" type="integer" optional="true" label="Gap extension penalty"/>
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345 </when>
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346 <when value="no">
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347 <!-- Do nothing -->
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348 </when>
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349 </conditional>
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350 <param name="ambigN" type="boolean" truevalue="--ambiguous=n" checked="false" label="Treat each N in the input sequences as an ambiguous nucleotide" argument="--ambiguous=n" help="Substitutions with N are scored as zero, instead of using the fill_score value from the scoring file (which is -100 by default)."/>
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351 <param name="ambigIUPAC" type="boolean" truevalue="--ambiguous=iupac" checked="false" label="Treat each of the IUPAC-IUB ambiguity codes (B, D, H, K, M, R, S, V, W, and Y, as well as N) in the input sequences as a completely ambiguous nucleotide." argument="--ambiguous=iupac" help="Substitutions with these characters are scored as zero, instead of using the fill_score value from the scoring file (which is -100 by default)."/>
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352 </section>
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353 <section name="seeding" expanded="false" title="Seeding">
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354 <conditional name="seed">
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355 <param name="seed_selector" type="select" display="radio" label="Select seed type">
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356 <option value="defaults" selected="true">Use defaults</option>
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357 <option value="pre_set">Use pre-set values</option>
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diff changeset
358 <option value="len">Set length</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
359 <option value="half_len">Set half length</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
360 <option value="pattern">Set pattern</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
361 </param>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
362 <when value="defaults">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
363 <!-- Do nothing -->
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
364 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
365 <when value="pre_set">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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366 <param name="pre_set_seeds" type="select" display="radio" label="Select seed pattern" help="Select between 12of19 seed (19-bp word with matches in 12 specific positions: 1110100110010101111) or 14of22 seed (22-bp word with matches in 14 specific positions: 1110101100110010101111). 0 = mismatch, 1 = match" argument="--seed=12of19,--seed=14of22">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
367 <option value="--seed=12of19">12of19</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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diff changeset
368 <option value="--seed=14of22">14of22</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
369 </param>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
370 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
371 <when value="len">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
372 <param name="seed_match" type="integer" optional="true" label="Set seed length" help="Seeds require a N-bp word with matches in all positions" argument="--seed=match" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
373 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
374 <when value="half_len">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
375 <param name="seed_half" type="integer" optional="true" label="Set seed length" help="Seeds requires N-bp word with matches or transitions in all positions" argument="--seed=half" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
376 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
377 <when value="pattern">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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378 <param name="pattern" type="text" size="25" optional="true" argument="--seed=PATTERN" label="Specify an arbitrary pattern" help="Use 1s, 0s, and Ts for seed discovery (e.g., 1110100110010101111 where 1 = match, 0 = mismatch, T = transition)" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
379 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
380 </conditional>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
381 <param name="transitions" type="select" display="radio" label="Allow transitions" help="In each seed, specify how many match positions can be transition(s) instead">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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382 <option value="--transition" selected="true">One</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
383 <option value="--transition=2">Two</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
384 <option value="--notransition">None</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
385 </param>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
386 <conditional name="seed_filer">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
387 <param name="seed_filer_selector" type="select" display="radio" label="Filter seeds" argument="--filter">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
388 <option value="yes">Yes</option>
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diff changeset
389 <option value="no" selected="true">No</option>
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parents:
diff changeset
390 </param>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
391 <when value="yes">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
392 <param name="filter_tr" type="integer" optional="true" label="Number of transitions" help="Allowing no more than this number of transversions. If not specified, any number of transversions is allowed (they are not limited)" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
393 <param name="filter_match" type="integer" optional="true" label="Number of matches" help="Require at least this many exact matches"/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
394 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
395 <when value="no">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
396 <!-- Do nothing -->
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parents:
diff changeset
397 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
398 </conditional>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
399 </section>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
400 <section name="hsp" expanded="false" title="HSPs (Gap-free extension)">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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401 <param name="gfextend" type="boolean" truevalue="--gfextend" checked="false" argument="--gfextend" label="Perform gap-free extension of seeds to HSPs" help="This will take into account other papermeters in this section"/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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402 <param name="nogfextend" type="boolean" truevalue="--nogfextend" argument="--nogfextend" label="Skip the gap-free extension stage" help="Instead, pass the seeds along to the next specified stage.It is not recommended to use --nogfextend without also using --nogapped."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
403 <conditional name="hsp_method">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
404 <param name="hsp_method_selector" type="select" display="radio" label="Select HSP finding method">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
405 <option value="none" selected="true">None</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
406 <option value="match">Match extension</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
407 <option value="mismatch">Mismatch extension</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
408 <option value="x">X-drop extension</option>
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parents:
diff changeset
409 </param>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
410 <when value="none">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
411 <!-- Do nothing -->
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
412 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
413 <when value="match">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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414 <param name="exact" type="integer" optional="true" argument="--exact" label="Find HSPs using the exact match extension method with the given length threshold" help="This is instead of using the x-drop method"/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
415 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
416 <when value="mismatch">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
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417 <param name="mismatch_count" type="integer" optional="true" label="Specify number of mismatches"/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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418 <param name="mismatch_length" type="integer" min="1" max="50" optional="true" label="Specify length threshold" help="Find HSPs using the mismatch extension method with the given length threshold and allowing specified number of mismatches" argument="--mismatch"/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
419 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
420 <when value="x">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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421 <param name="xdrop" type="integer" optional="true" argument="--xdrop" label="Find HSPs using the x-drop extension method with this threshold" help="The dropoff setting determines the endpoints of each gap-free segment: the extension of each seed is stopped when its cumulative score drops off by more than the given threshold from the maximum seen so far."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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422 <param name="hspthresh" type="integer" optional="true" argument="--hspthresh" label="Set the score threshold for the x-drop extension method" help="HSPs scoring lower are discarded"/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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diff changeset
423 <param name="hspthresh_top" type="integer" optional="true" argument="--hspthresh=top" label="Set an adaptive score threshold for the x-drop extension method" help="HSPs scoring lower are discarded. The score threshold is chosen to limit the number of target sequence bases in HSPs to about this value (or possibly a little higher in case of ties, etc.)."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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424 <param name="hspthresh_top_percent" type="integer" optional="true" argument="--hspthresh=top%" label="Set an adaptive score threshold for the x-drop extension method" help="HSPs scoring lower are discarded. The score threshold is chosen to limit the number of target sequence bases in HSPs to about this perentage value (or possibly a little higher in case of ties, etc.)."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
425 </when>
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diff changeset
426 </conditional>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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427 <param name="entropy" type="boolean" truevalue="--entropy" checked="false" label="Adjust for entropy when qualifying HSPs in the x-drop extension method" help="Those that score just slightly above the HSP threshold are adjusted downward according to the entropy of their nucleotides, and any that then fall below the threshold are discarded."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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428 <param name="entropy_report" type="boolean" truevalue="--entropy=report" checked="false" label="Adjust for entropy when qualifying HSPs in the x-drop extension method, and report (to stderr) any HSPs that are discarded as a result." help="Reported HSPs are printed to stderr"/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
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429 <param name="noentropy" type="boolean" truevalue="--noentropy" checked="false" label="Don't adjust for entropy when qualifying HSPs"/>
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diff changeset
430 </section>
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431 <section name="chaining" expanded="false" title="Chaining">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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432 <param name="chain" type="boolean" truevalue="--chain" checked="false" argument="--chain" label="Perform chaining of HSPs with no penalties"/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
433 <conditional name="chaning_penalties">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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434 <param name="chaning_penalties_selector" type="select" display="radio" argument="--chain=" label="Perform chaining with penalties">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
diff changeset
435 <option value="yes">Yes</option>
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parents:
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436 <option value="no" selected="true">No</option>
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diff changeset
437 </param>
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iuc
parents:
diff changeset
438 <when value="yes">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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parents:
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439 <param name="diag" type="integer" optional="true" label="Penalty for diagonal in dynamic programming matrix"/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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440 <param name="anti" type="integer" optional="true" label="Penalty for anti-diagonal in dynamic programming matrix" help="These are specified as positive values; subtraction from the score is implicitly assumed."/>
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441 </when>
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442 <when value="no">
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443 <!-- Do nothing -->
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444 </when>
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445 </conditional>
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446 </section>
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447 <section name="gap_ext" expanded="false" title="Gapped extension">
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448 <param name="gapped" type="boolean" truevalue="--gapped" argument="--gapped" label="Perform gapped extension of HSPs" help="Extension of HSPs (or seeds, if gap-free extension is not performed), is performed after first reducing them to anchor points."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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449 <param name="nogapped" type="boolean" truevalue="--nogapped" argument="--nogapped" label="Skip the gapped extension stage" help="This means that interpolation must also be skipped, since it is not allowed without gapped extension"/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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450 <param name="ydrop" type="integer" optional="true" argument="--ydrop" label="Set the threshold for terminating gapped extension" help="This restricts the endpoints of each local alignment by limiting the local region around each anchor in which extension is performed. The boundary of this region in the dynamic programming matrix is formed by the points where the cumulative score has dropped off by more than the given threshold from the maximum seen so far."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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451 <param name="noytrim" type="boolean" truevalue="--noytrim" argument="--noytrim" label="If y-drop extension encounters the end of the sequence, extend the alignment to the end of the sequence rather than trimming it back to the location giving the maximum score" help="This is highly recommended when either the target or query sequences are short reads (say, less than 100 bases), to prevent y-drop mismatch shadow."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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452 <param name="gappedthresh" type="integer" optional="true" argument="--gappedthresh" label="Set the threshold for gapped extension" help="Alignments scoring lower than that value are discarded. When used along with the x-drop method for gap-free extension, this value is generally set at least as high as the HSP threshold. Setting it lower has no effect, since at worst the HSP itself would always qualify (both extension stages use the same scoring matrix)."/>
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453 <param name="allgappedbounds" type="boolean" truevalue="--allgappedbounds" argument="--allgappedbounds" label="Revert to handling bounding alignments the way they were handled in BLASTZ."/>
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454 </section>
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455 <section name="filters" expanded="false" title="Filtering">
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456 <section name="identity" expanded="true" title="Filter alignments by percent identity">
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457 <param name="id_min" type="integer" min="0" max="100" optional="true" label="Minimum identity"/>
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458 <param name="id_max" type="integer" min="0" max="100" optional="true" argument="--filter=identity" label="Maximum identity (optional)" help="0 ≤ min ≤ max ≤ 100 percent. Identity is the percentage of aligned bases that are matches. Alignment blocks outside the given range are discarded."/>
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459 </section>
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460 <section name="continuity" expanded="false" title="Filter alignments by continuity">
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461 <param name="cont_min" type="integer" min="0" max="100" optional="true" label="Minimum continuity"/>
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462 <param name="cont_max" type="integer" min="0" max="100" optional="true" argument="--filter=continuity" label="Maximum continuity (optional)" help="0 ≤ min ≤ max ≤ 100 percent. Continuity is the percentage of alignment columns that are not gaps. Alignment blocks outside the given range are discarded."/>
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463 </section>
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464 <section name="coverage" expanded="false" title="Filter alignments by coverage">
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465 <param name="cov_min" type="integer" min="0" max="100" optional="true" label="Minimum coverage"/>
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466 <param name="cov_max" type="integer" min="0" max="100" optional="true" argument="--filter=coverage" label="Maximum coverage (optional)" help=" 0 ≤ min ≤ max ≤ 100 percent. Coverage is the percentage of the entire target or query sequence (whichever is shorter) that is included in the alignment block. Blocks outside the given range are discarded."/>
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467 </section>
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468 <param name="filter_nmatch" type="integer" min="1" optional="true" argument="--filter=nmatch" label="Filter alignments by how many bases match" help="Requiring at least this number of matched bases, min > 0. Match count is the number of matched bases in the alignment."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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469 <param name="filter_nmatch_percent" type="integer" min="1" optional="true" argument="--filter=nmatch%" label="Filter alignments by how many bases match expressed as percentage" help="e.g., percentage of the query length."/>
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470 <param name="filter_nmismatch" type="integer" min="0" optional="true" argument="--filter=nmismatch:0.." label="Filter alignments by the number of mismatches" help="Allow no more than this number of mismatched bases. Mismatch count, or nmismatch, is the number of aligned bases in the alignment that are mismatches (substitutions)."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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471 <param name="filter_ngap" type="integer" min="0" optional="true" argument="--filter=ngap:0.." label="Filter alignments by the number of gaps" help="Allow no more than this number of gaps. Gap count, or ngap, is the number of runs of gapped columns in the alignment (each run is counted as one gap)."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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472 <param name="filter_cgap" type="integer" min="0" optional="true" argument="--filter=cgap:0.." label="Filter alignments by the number of gap columns" help="Allow no more than this number of gaps. Gap column count, or cgap, is the number of gapped columns in the alignment (each column is counted as one gap)."/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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473 <param name="notrivial" type="boolean" truevalue="--notrivial" argument="--notrivial" label="Do not output a trivial self-alignment block if the target and query sequences are identical" help="Note that using ‑‑self automatically enables this option."/>
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474 </section>
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475 <section name="interpolation" expanded="false" title="Interpolation">
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476 <param name="inner" type="integer" optional="true" argument="--inner" label="Perform additional alignment between the gapped alignment blocks, using (presumably) more sensitive alignment parameters" help="This value is used as the threshold for both the gap-free and gapped extension sub-stages; see the discussion of interpolation for more details. This option is only valid if gapped extension is performed."/>
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477 </section>
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478 <section name="output_format" expanded="false" title="Output">
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479 <conditional name="out">
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480 <param name="format" type="select" display="radio" label="Specify the output format">
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481 <option value="bam" selected="true">BAM --format=sam)</option>
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482 <option value="general_def">General default (--format=general)</option>
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483 <option value="general_full">Customized general (‑‑format=general[:fields])</option>
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484 <option value="maf">MAF (--format=maf)</option>
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485 <option value="blastn">blastn (--format=BLASTN)</option>
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486 </param>
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487 <when value="bam">
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488 <param name="bam_options" type="select" display="radio" argument="--format=sam, --format=softsam" label="Select a BAM flavor to output" help="Lastz actually outputs SAM data but Galaxy converts it into BAM to save space. For alignments that don't reach the end of a query, ‑‑format=sam uses 'hard clipping', while ‑‑format=softsam uses 'soft clipping'. See the section on 'clipped alignment' in the SAM specification for an explanation of what this means. The options ‑‑format=sam- and ‑‑format=softsam- suppress the SAM header lines. This makes them suitable for concatenating output from multiple runs. If you need to specify readgroup information: use AddOrEplaceReadGroups from Picard package">
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489 <option value="sam" selected="true">BAM</option>
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490 <option value="softsam">soft-clipped BAM</option>
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491 <option value="sam-">BAM without header</option>
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492 <option value="softsam-">soft-clipped BAM without header</option>
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493 </param>
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494 </when>
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495 <when value="general_def">
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496 <!-- Do nothing -->
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497 </when>
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498 <when value="general_full">
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499 <param name="fields" type="select" display="checkboxes" multiple="true" label="Select which fields to include" argument="--format=general-[:fields]">
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500 <option value="score" selected="true">score: Score of the alignment block</option>
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501 <option value="name1" selected="true">name1: Name of the target sequence</option>
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502 <option value="number1">number1: Number of the target sequence within the target file</option>
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503 <option value="strand1" selected="true">strand1: Target sequence strand </option>
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504 <option value="size1" selected="true">size1: Size of the entire target sequence</option>
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505 <option value="start1">start1: Starting position of the alignment block in the target, origin-one</option>
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506 <option value="zstart1" selected="true">zstart1: Starting position of the alignment block in the target, origin-zero</option>
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507 <option value="end1" selected="true">end1: Ending position of the alignment block in the target</option>
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508 <option value="length1">length1: Length of the alignment block in the target (excluding gaps)</option>
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509 <option value="text1">text1: Aligned characters in the target, including gap characters</option>
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510 <option value="qalign1">qalign1: The target quality sequence (if there is one) correpsonding to aligned characters</option>
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511 <option value="nucs1">nucs1: The entire target sequence</option>
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512 <option value="name2" selected="true">name2: Name of the query sequence</option>
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513 <option value="number2">number2: Number of the query sequence within the query file</option>
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514 <option value="strand2" selected="true">strand2: Query sequence strand</option>
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515 <option value="size2" selected="true">size2: Size of the entire query sequence</option>
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iuc
parents:
diff changeset
516 <option value="start2">start2: Starting position of the alignment block in the query, origin-one</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
517 <option value="zstart2" selected="true">zstart2: Starting position of the alignment block in the query, origin-one</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
518 <option value="end2" selected="true">end2: Ending position of the alignment block in the query</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
519 <option value="length2">length2: Length of the alignment block in the query (excluding gaps)</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
520 <option value="text2">text2: Aligned characters in the query, including gap characters</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
521 <option value="qalign2">qalign2: The query quality sequence (if there is one) correpsonding to aligned characters</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
522 <option value="nucs2">nucs2: The entire query sequence</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
523 <option value="nmatch">nmatch: Match count</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
524 <option value="nmismatch">nmismatch: Mismatch count</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
525 <option value="ncolumn">ncolumn: Number of columns in the block. This includes matches, mismatches (substitutions), and gaps</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
526 <option value="npair">npair: Number of aligned bases in the block that are matches or mismatches (substitutions)</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
527 <option value="ngap">ngap: Gap count, the number of gaps in the block, counting each run of gapped columns as a single gap</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
528 <option value="cgap">cgap: Gap column count, the number of gaps in the block, counting each gapped column as a separate gap</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
529 <option value="diff">diff: Differences between what would be written for text1 and text2</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
530 <option value="cigar">cigar: A CIGAR-like representation of the alignment’s path</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
531 <option value="cigarx">cigarx: Same as cigar, but uses a newer syntax that distinguishes matches from substitutions</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
532 <option value="identity" selected="true">identity: Fraction of aligned bases in the block that are matches </option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
533 <option value="idfrac">idfrac: Fraction of aligned bases in the block that are matches </option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
534 <option value="id%" selected="true">id% Fraction of aligned bases in the block that are matches (as %)</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
535 <option value="blastid%">blastid%: Fraction of the alignment block that is matches, as would be reported by NCBI BLAST</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
536 <option value="continuity">continuity: Rate of non-gaps (non-indels) in the alignment block</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
537 <option value="confrac">confrac: Rate of non-gaps (non-indels) in the alignment block (as fraction)</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
538 <option value="con%">con%: Rate of non-gaps (non-indels) in the alignment block (as %)</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
539 <option value="coverage" selected="true">coverage: Fraction of the entire input sequence (target or query, whichever is shorter) that is covered by the alignment block</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
540 <option value="covfrac">covfrac: Fraction of the entire input sequence (target or query, whichever is shorter) that is covered by the alignment block (as fraction)</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
541 <option value="cov%" selected="true">cov%: Fraction of the entire input sequence (target or query, whichever is shorter) that is covered by the alignment block (as %)</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
542 <option value="diagonal">diagonal: The diagonal of the start of the alignment block in the DP matrix, expressed as an identifying number start1-start2</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
543 <option value="shingle">shingle: A measurement of the shingle overlap between the target and the query</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
544 <option value="number">number: The alignment number, counted as alignments are written to output (1-base)</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
545 <option value="znumber">znumber: The alignment number, counted as alignments are written to output (0-base)</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
546 <sanitizer invalid_char="">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
547 <valid initial="string.letters,string.digits"><add value="%" /> </valid>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
548 </sanitizer>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
549 </param>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
550 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
551 <when value="maf">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
552 <param name="maf_type" type="select" display="radio" argument="--format=maf" label="Seleat MAF flavor" help="MAF is a multiple alignment format developed at UCSC">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
553 <option value="maf">MAF</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
554 <option value="maf+">MAF with additional stats</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
555 <option value="maf-">MAF without header and comments</option>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
556 </param>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
557 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
558 <when value="blastn">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
559 <!-- Do nothing -->
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
560 </when>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
561 </conditional>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
562 </section>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
563 </inputs>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
564 <outputs>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
565 <data format="tabular" name="output" label="${tool.name} on ${on_string}: mapped reads">
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
566 <change_format>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
567 <when input="output_format.out.format" value="bam" format="bam" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
568 <when input="output_format.out.format" value="maf" format="maf" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
569 </change_format>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
570 </data>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
571 <data format="png" name="out_plot" label="${tool.name} on ${on_string}: dot plot"/>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
572 </outputs>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
573 <tests>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
574 <test>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
575 <param name="ref_source" value="cached" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
576 <param name="target_2bit" value="phiX174" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
577 <param name="query" value="phiX_split.fasta" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
578 <param name="strand" value="--strand=both" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
579 <param name="format" value="general_def" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
580 <output name="output" value="test1.out" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
581 </test>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
582 <test>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
583 <param name="ref_source" value="history" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
584 <param name="target" value="phiX_split.fasta" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
585 <param name="query" value="phiX.fasta" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
586 <param name="strand" value="--strand=both" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
587 <param name="format" value="general_def" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
588 <output name="output" value="test2.out" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
589 </test>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
590 <test>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
591 <param name="ref_source" value="history" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
592 <param name="target" value="phiX_split.fasta" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
593 <param name="query" value="phiX.fasta" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
594 <param name="strand" value="--strand=both" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
595 <param name="score_file" value="score_file.txt" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
596 <param name="format" value="general_def" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
597 <output name="output" value="test3.out" />
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
598 </test>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
599 </tests>
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
600
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
601 <help><![CDATA[
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
602
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
parents:
diff changeset
603 **What is does**
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604
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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605 LASTZ is designed to preprocess one sequence or set of sequences (which we collectively call the *TARGET*) and then align several *QUERY* sequences to it. It was developed by `Bob Harris <http://www.bx.psu.edu/~rsharris/>`_ in the lab of Webb Miller at Penn State.
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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606
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607 .. class:: warningmark
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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608
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609 **Read documentation** before proceeding. LASTZ is a complex tool with many parameter options. Fortunately, there is a `great manual <https://lastz.github.io/lastz/>`_ maintained by its author. Default parameters may be sufficient to obtain the initial idea about how similar your sequences are, but to produce reliable alignments you may need to tweak the parameters. So RTFM!
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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610
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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611 **About LASTZ parameters**
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612
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613 Galaxy's version of LASTZ has nine parameter sections (*Where to look*, *Scoring*, *Seeding*, *HSPs*, *Chaining*, *Gapped extension*, *Filtering*, *Interpolation*, and *Output*). These sections closely follow parameter description in the `manual <https://lastz.github.io/lastz/#syntax>`_.
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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614
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615 **Defaults**
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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616
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617 here are defaults for some of the most important parameters::
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618
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619 --seed=<pattern> set seed pattern (12of19, 14of22, or general pattern)
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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620 (default is 1110100110010101111)
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621 SEE "Seeding" SECTION -> "Select seed type"
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622
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623 --[no]transition allow (or don't) one transition in a seed hit
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624 (by default a transition is allowed)
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625 SEE "Seeding" SECTION -> "Allow transitions"
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626
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627 --[no]chain perform chaining
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628 (by default no chaining is performed)
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629 SEE "Chaining" SECTION
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630
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631 --[no]gapped perform gapped alignment (instead of gap-free)
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632 (by default gapped alignment is performed)
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633 SEE "Gapped extension" SECTION
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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634
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635 --strand=both search both strands
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636 --strand=plus search + strand only (matching strand of query spec)
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637 (by default both strands are searched)
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638 SEE "Where to look" SECTION
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639
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640 --scores=<file> read substitution and gap scores from a file
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641 SEE "Scoring" SECTION
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642
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643 --xdrop=<score> set x-drop threshold (default is 10sub[A][A])
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644 SEE "HSPs" SECTION
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645
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646 --ydrop=<score> set y-drop threshold (default is open+300extend)
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647 SEE "Gapped extension" SECTION
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diff changeset
648
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649 --hspthresh=<score> set threshold for high scoring pairs (default is 3000)
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650 ungapped extensions scoring lower are discarded
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diff changeset
651 <score> can also be a percentage or base count
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iuc
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diff changeset
652 SEE "HSPs" SECTION
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diff changeset
653
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diff changeset
654 --gappedthresh=<score> set threshold for gapped alignments
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
655 gapped extensions scoring lower are discarded
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
656 <score> can also be a percentage or base count
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diff changeset
657 (default is to use same value as --hspthresh)
8e9252994649 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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diff changeset
658 SEE "Gapped extension" SECTION
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659
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diff changeset
660
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diff changeset
661 **Substitution matrix**
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662
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663 By default the HOXD70 substitution scores are used (from `Chiaromonte et al. 2002 <https://www.ncbi.nlm.nih.gov/pubmed/11928468>`_)::
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664
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diff changeset
665 bad_score = X:-1000 # used for sub['X'][*] and sub[*]['X']
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666 fill_score = -100 # used when sub[*][*] is not defined
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667 gap_open_penalty = 400
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668 gap_extend_penalty = 30
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669
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670 A C G T
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671 A 91 -114 -31 -123
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672 C -114 100 -125 -31
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673 G -31 -125 100 -114
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674 T -123 -31 -114 91
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675
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676 Matrix can be supplied as an input to **Read the substitution scores** parameter in *Scoring* section. Substitution matrix can be inferred from your data using another LASTZ-based tool (LASTZ_D: Infer substitution scores).
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677
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678 **Output**
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679
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diff changeset
680 This version of LASTZ produces two outputs by default: a BAM alignment file and a dot-plot in PNG format. Other formats can be configured in *Output* section. This incarnation of LASTZ produces outputs without comment line starting with '#'. To learn identity of each column, consult `formats section of LASTZ manual <https://lastz.github.io/lastz/#formats>`_.
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681
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682 ]]>
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683 </help>
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684 <expand macro="citations"/>
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685 </tool>