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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/blob/master/tool_collections/kraken/ commit 1590e274eaa41ea77a6017111f9122e0a58aa75d-dirty"
author iuc
date Tue, 03 Dec 2019 18:29:41 +0000
parents 96f11a194da9
children
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1 <?xml version="1.0"?>
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2 <tool id="kraken" name="Kraken" version="@WRAPPER_VERSION@">
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3 <description>
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4 assign taxonomic labels to sequencing reads
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5 </description>
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6 <macros>
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7 <import>macros.xml</import>
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8 </macros>
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9 <expand macro="requirements" />
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10 <expand macro="version_command" />
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11 <command detect_errors="exit_code">
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12 <![CDATA[
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13 @SET_DATABASE_PATH@ &&
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15 kraken
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16 --threads \${GALAXY_SLOTS:-1}
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17 @INPUT_DATABASE@
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18 ${only_classified_output}
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20 #if str( $quick_operation.quick ) == "yes":
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21 --quick
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22 --min-hits ${quick_operation.min_hits}
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24 #end if
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26 #if $single_paired.single_paired_selector == 'yes'
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27 #if $forward_input.is_of_type( 'fastq' ):
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28 --fastq-input
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29 #else:
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30 --fasta-input
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31 #end if
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32 '${single_paired.forward_input}' '${single_paired.reverse_input}'
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33 ${single_paired.check_names}
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34 #elif $single_paired.single_paired_selector == "collection":
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35 #if $single_paired.input_pair.forward.is_of_type( 'fastq' ):
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36 --fastq-input
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37 #else:
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38 --fasta-input
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39 #end if
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40 '${single_paired.input_pair.forward}' '${single_paired.input_pair.reverse}'
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41 ${single_paired.check_names}
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42 #else:
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43 #if $single_paired.input_sequences.is_of_type('fastq')
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44 --fastq-input
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45 #else:
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46 --fasta-input
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47 #end if
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48 '${single_paired.input_sequences}'
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49 #end if
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51 #if $split_reads:
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52 --classified-out '${classified_out}' --unclassified-out '${unclassified_out}'
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53 #end if
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54
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55 ## The --output option was changed to redirect as it does not work properly is some situations. For example, on test database the tool classifies 4 reads but does not write them into a file if --output is specified. It does however print correct output into STDOUT. This behavior can be re-created with test database provided in test-data/test_db/ folder. This is the reason for incrementing version number from 1.1.2 to 1.1.3
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56
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57 > '${output}'
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58 ##kraken-translate --db '${kraken_database.fields.name}' '${output}' > '${translated}'
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59 ]]></command>
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60 <inputs>
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61 <conditional name="single_paired">
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62 <param name="single_paired_selector" type="select" label="Single or paired reads" help="--paired">
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63 <option value="collection">Collection</option>
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64 <option value="yes">Paired</option>
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65 <option selected="True" value="no">Single</option>
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66 </param>
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67 <when value="collection">
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68 <param format="fasta,fastq" name="input_pair" type="data_collection" collection_type="paired" label="Collection of paired reads" help="FASTA or FASTQ datasets" />
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69 <param name="check_names" argument="--check-names" type="boolean" checked="False" truevalue="--paired --check-names" falsevalue="--paired" label="Verify read names match"/>
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70 </when>
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71 <when value="yes">
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72 <param format="fasta,fastq" name="forward_input" type="data" label="Forward strand" help="FASTA or FASTQ dataset"/>
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73 <param format="fasta,fastq" name="reverse_input" type="data" label="Reverse strand" help="FASTA or FASTQ dataset"/>
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74 <param name="check_names" type="boolean" checked="False" truevalue="--paired --check-names" falsevalue="--paired" label="Verify read names match" help="--check-names" />
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75 </when>
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76 <when value="no">
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77 <param format="fasta,fastq" label="Input sequences" name="input_sequences" type="data" help="FASTA or FASTQ datasets"/>
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78 </when>
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79
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80 </conditional>
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81 <param name="split_reads" type="boolean" label="Output classified and unclassified reads?" help="Sets --unclassified-out and --classified-out"/>
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82
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83 <conditional name="quick_operation">
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84 <param argument="--quick" type="select" label="Enable quick operation"
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85 help="Quick mode: rather than searching all k-mers in a sequence, stop classification after a specified number of database hit">
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86 <option value="yes">Yes</option>
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87 <option selected="True" value="no">No</option>
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88 </param>
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89 <when value="yes">
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90 <param name="min_hits" argument="--min-hits" type="integer" value="1" label="Number of hits required for classification"
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91 help="min-hits will allow you to require multiple hits before declaring a sequence classified, which can be especially useful with custom databases when testing to see if sequences either do or do not belong to a particular genome; default=1"/>
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92 </when>
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93 <when value="no"/><!-- Do absolutely nothing -->
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94 </conditional>
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95
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96 <param name="only_classified_output" argument="--only-classified-output" type="boolean" checked="False" truevalue="--only-classified-output" falsevalue=""
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97 label="Print no Kraken output for unclassified sequences"/>
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98
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99 <expand macro="input_database" />
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100 </inputs>
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101 <outputs>
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102 <data name="classified_out" format_source="input_sequences" label="${tool.name} on ${on_string}: Classified reads">
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103 <filter>(split_reads)</filter>
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104 </data>
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105 <data name="unclassified_out" format_source="input_sequences" label="${tool.name} on ${on_string}: Unclassified reads">
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106 <filter>(split_reads)</filter>
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107 </data>
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108 <data name="output" format="tabular" label="${tool.name} on ${on_string}: Classification"/>
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109 <!--<data format="tabular" label="${tool.name} on ${on_string}: Translated classification" name="translated" />-->
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110 </outputs>
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111
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112 <tests>
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113 <test>
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114 <param name="single_paired_selector" value="no"/>
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115 <param name="input_sequences" value="kraken/kraken_test1.fa" ftype="fasta"/>
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116 <param name="split_reads" value="false"/>
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117 <param name="quick" value="no"/>
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118 <param name="only-classified-output" value="false"/>
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119 <param name="kraken_database" value="old_style_test_entry"/>
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120 <output name="output" file="kraken/kraken_test1_output.tab" ftype="tabular"/>
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121 </test>
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122 <test>
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123 <param name="single_paired_selector" value="no"/>
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124 <param name="input_sequences" value="kraken/kraken_test1.fa" ftype="fasta"/>
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125 <param name="split_reads" value="false"/>
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126 <param name="quick" value="no"/>
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127 <param name="only-classified-output" value="false"/>
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128 <param name="kraken_database" value="new_style_test_entry"/>
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129 <output name="output" file="kraken/kraken_test1_output.tab" ftype="tabular"/>
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130 </test>
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131 </tests>
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132 <help>
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133 <![CDATA[
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134 **What it does**
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135
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136 Kraken is a taxonomic sequence classifier that assigns taxonomic labels to short DNA reads. It does this by examining the k-mers within a read and querying a database with those k-mers. This database contains a mapping of every k-mer in Kraken's genomic library to the lowest common ancestor (LCA) in a taxonomic tree of all genomes that contain that k-mer. The set of LCA taxa that correspond to the k-mers in a read are then analyzed to create a single taxonomic label for the read; this label can be any of the nodes in the taxonomic tree. Kraken is designed to be rapid, sensitive, and highly precise.
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137
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138 -----
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139
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140 **Output Format**
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141
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142 Each sequence classified by Kraken results in a single line of output. Output lines contain five tab-delimited fields; from left to right, they are::
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143
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144 1. "C"/"U": one letter code indicating that the sequence was either classified or unclassified.
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145 2. The sequence ID, obtained from the FASTA/FASTQ header.
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146 3. The taxonomy ID Kraken used to label the sequence; this is 0 if the sequence is unclassified.
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147 4. The length of the sequence in bp.
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148 5. A space-delimited list indicating the LCA mapping of each k-mer in the sequence. For example, "562:13 561:4 A:31 0:1 562:3" would indicate that:
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149 a) the first 13 k-mers mapped to taxonomy ID #562
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150 b) the next 4 k-mers mapped to taxonomy ID #561
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151 c) the next 31 k-mers contained an ambiguous nucleotide
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152 d) the next k-mer was not in the database
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153 e) the last 3 k-mers mapped to taxonomy ID #562
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154 ]]>
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155 </help>
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156 <expand macro="citations" />
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157 </tool>