diff hilbertvis.sh @ 0:6f6859904b65 draft

Uploaded tool tarball.
author devteam
date Tue, 20 Aug 2013 10:57:23 -0400
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/hilbertvis.sh	Tue Aug 20 10:57:23 2013 -0400
@@ -0,0 +1,109 @@
+#!/usr/bin/env bash
+
+input_file="$1"
+output_file="$2"
+chromInfo_file="$3"
+chrom="$4"
+score_col="$5"
+hilbert_curve_level="$6"
+summarization_mode="$7"
+chrom_col="$8"
+start_col="$9"
+end_col="${10}"
+strand_col="${11}"
+
+## use first sequence if chrom filed is empty
+if [ -z "$chrom" ]; then
+    chrom=$( head -n 1 "$input_file" | cut -f$chrom_col )
+fi
+
+## get sequence length 
+if [ ! -r "$chromInfo_file" ]; then
+    echo "Unable to read chromInfo_file $chromInfo_file" 1>&2
+    exit 1
+fi
+
+chrom_len=$( awk '$1 == chrom {print $2}' chrom=$chrom $chromInfo_file )
+
+## error if we can't find the chrom_len
+if [ -z "$chrom_len" ]; then
+    echo "Can't find length for sequence \"$chrom\" in chromInfo_file $chromInfo_file" 1>&2
+    exit 1
+fi
+
+## make sure chrom_len is positive
+if [ $chrom_len -le 0 ]; then
+    echo "sequence \"$chrom\" length $chrom_len <= 0" 1>&2
+    exit 1
+fi
+
+## modify R script depending on the inclusion of a score column, strand information
+input_cols="\$${start_col}, \$${end_col}"
+col_types='beg=0, end=0, strand=""'
+
+# if strand_col == 0 (strandCol metadata is not set), assume everything's on the plus strand
+if [ $strand_col -ne 0 ]; then
+    input_cols="${input_cols}, \$${strand_col}"
+else
+    input_cols="${input_cols}, \\\"+\\\""
+fi
+
+# set plot value (either from data or use a constant value)
+if [ $score_col -eq -1 ]; then
+    value=1
+else
+    input_cols="${input_cols}, \$${score_col}"
+    col_types="${col_types}, score=0"
+    value='chunk$score[i]'
+fi
+
+R --vanilla &> /dev/null <<endOfCode
+library(HilbertVis);
+
+chrom_len <- ${chrom_len};
+chunk_size <- 1000;
+interval_count <- 0;
+invalid_strand <- 0;
+
+awk_cmd <- paste(
+  "awk 'BEGIN{FS=\"\t\";OFS=\"\t\"}",
+    "\$${chrom_col} == \"${chrom}\"",
+      "{print ${input_cols}}' ${input_file}"
+);
+
+col_types <- list(${col_types});
+vec <- vector(mode="numeric", length=chrom_len);
+conn <- pipe(description=awk_cmd, open="r");
+
+repeat {
+  chunk <- scan(file=conn, what=col_types, sep="\t", nlines=chunk_size, quiet=TRUE);
+
+  if ((rows <- length(chunk\$beg)) == 0)
+        break;
+
+  interval_count <- interval_count + rows;
+
+  for (i in 1:rows) {
+    if (chunk\$strand[i] == '+') {
+      start <- chunk\$beg[i] + 1;
+      stop <- chunk\$end[i];
+    } else if (chunk\$strand[i] == '-') {
+      start <- chrom_len - chunk\$end[i] - 1;
+      stop <- chrom_len - chunk\$beg[i];
+    } else {
+      invalid_strand <- invalid_strand + 1;
+      interval_count <- interval_count - 1;
+      next;
+    }
+    vec[start:stop] <- ${value};
+  }
+}
+
+close(conn);
+
+hMat <- hilbertImage(vec, level=$hilbert_curve_level, mode="$summarization_mode");
+pdf(file="$output_file", onefile=TRUE, width=8, height=10.5, paper="letter");
+showHilbertImage(hMat);
+dev.off();
+endOfCode
+