Mercurial > repos > devteam > hgv_hilbertvis
diff hilbertvis.sh @ 0:6f6859904b65 draft
Uploaded tool tarball.
author | devteam |
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date | Tue, 20 Aug 2013 10:57:23 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/hilbertvis.sh Tue Aug 20 10:57:23 2013 -0400 @@ -0,0 +1,109 @@ +#!/usr/bin/env bash + +input_file="$1" +output_file="$2" +chromInfo_file="$3" +chrom="$4" +score_col="$5" +hilbert_curve_level="$6" +summarization_mode="$7" +chrom_col="$8" +start_col="$9" +end_col="${10}" +strand_col="${11}" + +## use first sequence if chrom filed is empty +if [ -z "$chrom" ]; then + chrom=$( head -n 1 "$input_file" | cut -f$chrom_col ) +fi + +## get sequence length +if [ ! -r "$chromInfo_file" ]; then + echo "Unable to read chromInfo_file $chromInfo_file" 1>&2 + exit 1 +fi + +chrom_len=$( awk '$1 == chrom {print $2}' chrom=$chrom $chromInfo_file ) + +## error if we can't find the chrom_len +if [ -z "$chrom_len" ]; then + echo "Can't find length for sequence \"$chrom\" in chromInfo_file $chromInfo_file" 1>&2 + exit 1 +fi + +## make sure chrom_len is positive +if [ $chrom_len -le 0 ]; then + echo "sequence \"$chrom\" length $chrom_len <= 0" 1>&2 + exit 1 +fi + +## modify R script depending on the inclusion of a score column, strand information +input_cols="\$${start_col}, \$${end_col}" +col_types='beg=0, end=0, strand=""' + +# if strand_col == 0 (strandCol metadata is not set), assume everything's on the plus strand +if [ $strand_col -ne 0 ]; then + input_cols="${input_cols}, \$${strand_col}" +else + input_cols="${input_cols}, \\\"+\\\"" +fi + +# set plot value (either from data or use a constant value) +if [ $score_col -eq -1 ]; then + value=1 +else + input_cols="${input_cols}, \$${score_col}" + col_types="${col_types}, score=0" + value='chunk$score[i]' +fi + +R --vanilla &> /dev/null <<endOfCode +library(HilbertVis); + +chrom_len <- ${chrom_len}; +chunk_size <- 1000; +interval_count <- 0; +invalid_strand <- 0; + +awk_cmd <- paste( + "awk 'BEGIN{FS=\"\t\";OFS=\"\t\"}", + "\$${chrom_col} == \"${chrom}\"", + "{print ${input_cols}}' ${input_file}" +); + +col_types <- list(${col_types}); +vec <- vector(mode="numeric", length=chrom_len); +conn <- pipe(description=awk_cmd, open="r"); + +repeat { + chunk <- scan(file=conn, what=col_types, sep="\t", nlines=chunk_size, quiet=TRUE); + + if ((rows <- length(chunk\$beg)) == 0) + break; + + interval_count <- interval_count + rows; + + for (i in 1:rows) { + if (chunk\$strand[i] == '+') { + start <- chunk\$beg[i] + 1; + stop <- chunk\$end[i]; + } else if (chunk\$strand[i] == '-') { + start <- chrom_len - chunk\$end[i] - 1; + stop <- chrom_len - chunk\$beg[i]; + } else { + invalid_strand <- invalid_strand + 1; + interval_count <- interval_count - 1; + next; + } + vec[start:stop] <- ${value}; + } +} + +close(conn); + +hMat <- hilbertImage(vec, level=$hilbert_curve_level, mode="$summarization_mode"); +pdf(file="$output_file", onefile=TRUE, width=8, height=10.5, paper="letter"); +showHilbertImage(hMat); +dev.off(); +endOfCode +