# HG changeset patch # User devteam # Date 1444754492 14400 # Node ID 76cefe061c3d3ef5e8e077e35e0da5cb14aedbb5 # Parent 8c60fa8ae153c8bbf45d8941ab7394c2ac912594 planemo upload commit 33927a87ba2eee9bf0ecdd376a66241b17b3d734 diff -r 8c60fa8ae153 -r 76cefe061c3d fastx_quality_statistics.xml --- a/fastx_quality_statistics.xml Tue Nov 26 12:49:06 2013 -0500 +++ b/fastx_quality_statistics.xml Tue Oct 13 12:41:32 2015 -0400 @@ -1,27 +1,27 @@ - + fastx_toolkit - zcat -f $input | fastx_quality_stats -o $output -Q 33 - - - - + + + - - - - - - - - - - - - - + + + + + + + + + + + + + **What it does** Creates quality statistics report for the given Solexa/FASTQ library. @@ -34,23 +34,23 @@ **The output file will contain the following fields:** -* column = column number (1 to 36 for a 36-cycles read Solexa file) +* column = column number (1 to 36 for a 36-cycles read Solexa file) * count = number of bases found in this column. * min = Lowest quality score value found in this column. * max = Highest quality score value found in this column. * sum = Sum of quality score values for this column. * mean = Mean quality score value for this column. -* Q1 = 1st quartile quality score. -* med = Median quality score. -* Q3 = 3rd quartile quality score. -* IQR = Inter-Quartile range (Q3-Q1). -* lW = 'Left-Whisker' value (for boxplotting). -* rW = 'Right-Whisker' value (for boxplotting). -* A_Count = Count of 'A' nucleotides found in this column. -* C_Count = Count of 'C' nucleotides found in this column. -* G_Count = Count of 'G' nucleotides found in this column. -* T_Count = Count of 'T' nucleotides found in this column. -* N_Count = Count of 'N' nucleotides found in this column. +* Q1 = 1st quartile quality score. +* med = Median quality score. +* Q3 = 3rd quartile quality score. +* IQR = Inter-Quartile range (Q3-Q1). +* lW = 'Left-Whisker' value (for boxplotting). +* rW = 'Right-Whisker' value (for boxplotting). +* A_Count = Count of 'A' nucleotides found in this column. +* C_Count = Count of 'C' nucleotides found in this column. +* G_Count = Count of 'G' nucleotides found in this column. +* T_Count = Count of 'T' nucleotides found in this column. +* N_Count = Count of 'N' nucleotides found in this column. For example:: @@ -60,13 +60,12 @@ 3 6362991 -5 40 248722469 39.09 40 40 40 0 40 40 2296384 984875 1443989 1637743 0 4 6362991 -4 40 248214827 39.01 40 40 40 0 40 40 2536861 1167423 1248968 1409739 0 36 6362991 -5 40 117158566 18.41 7 15 30 23 -5 40 4074444 1402980 63287 822035 245 - + ------ This tool is based on `FASTX-toolkit`__ by Assaf Gordon. .. __: http://hannonlab.cshl.edu/fastx_toolkit/ - - + diff -r 8c60fa8ae153 -r 76cefe061c3d tool_dependencies.xml --- a/tool_dependencies.xml Tue Nov 26 12:49:06 2013 -0500 +++ b/tool_dependencies.xml Tue Oct 13 12:41:32 2015 -0400 @@ -1,6 +1,6 @@ - +